Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024043.01.T01 | XP_024640952.1 | 100 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 1.81E-64 | 206 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024043.01.T01 | G7ZVA3 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 9.75e-65 | 206 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048352.01 | MsG0580024043.01 | 0.807648 | 4.561710e-50 | 2.554612e-47 |
| MsG0180004941.01 | MsG0580024043.01 | 0.819058 | 1.417359e-52 | 1.079296e-49 |
| MsG0180005356.01 | MsG0580024043.01 | 0.814597 | 1.421040e-51 | 9.575934e-49 |
| MsG0180005357.01 | MsG0580024043.01 | 0.804776 | 1.836203e-49 | 9.550794e-47 |
| MsG0280011165.01 | MsG0580024043.01 | 0.815256 | 1.014620e-51 | 6.960127e-49 |
| MsG0480023107.01 | MsG0580024043.01 | 0.816047 | 6.764235e-52 | 4.742241e-49 |
| MsG0480023267.01 | MsG0580024043.01 | 0.826325 | 2.886697e-54 | 2.698498e-51 |
| MsG0480023303.01 | MsG0580024043.01 | 0.817635 | 2.977410e-52 | 2.179990e-49 |
| MsG0480023819.01 | MsG0580024043.01 | 0.801225 | 9.955334e-49 | 4.728829e-46 |
| MsG0580024043.01 | MsG0580024218.01 | 0.813155 | 2.953085e-51 | 1.913556e-48 |
| MsG0580024043.01 | MsG0580024219.01 | 0.800895 | 1.162807e-48 | 5.477804e-46 |
| MsG0580024043.01 | MsG0580028999.01 | 0.810987 | 8.770562e-51 | 5.365503e-48 |
| MsG0580024043.01 | MsG0580030145.01 | 0.803858 | 2.852604e-49 | 1.449088e-46 |
| MsG0580024043.01 | MsG0780036661.01 | 0.812399 | 4.323832e-51 | 2.746445e-48 |
| MsG0580024043.01 | MsG0780039576.01 | 0.800935 | 1.140915e-48 | 5.380081e-46 |
| MsG0580024043.01 | MsG0780039978.01 | 0.814852 | 1.247298e-51 | 8.463141e-49 |
| MsG0580024043.01 | MsG0780041556.01 | 0.804715 | 1.890905e-49 | 9.819881e-47 |
| MsG0580024043.01 | MsG0880045677.01 | 0.804347 | 2.256929e-49 | 1.160955e-46 |
| MsG0380016801.01 | MsG0580024043.01 | 0.809582 | 1.761852e-50 | 1.038467e-47 |
| MsG0280008405.01 | MsG0580024043.01 | 0.807146 | 5.828312e-50 | 3.221677e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024043.01.T01 | MTR_5g004870 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 2.47e-68 | 206 |
| MsG0580024043.01.T01 | MTR_5g004800 | 84.444 | 90 | 14 | 0 | 3 | 92 | 7 | 96 | 4.81e-54 | 168 |
| MsG0580024043.01.T01 | MTR_1g008950 | 28.049 | 82 | 59 | 0 | 11 | 92 | 6 | 87 | 1.94e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024043.01.T01 | AT4G34150 | 76.136 | 88 | 21 | 0 | 2 | 89 | 3 | 90 | 7.10e-45 | 145 |
Find 25 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCACGAAGGCCTTCAATT+AGG | 0.223666 | 5:-213087 | None:intergenic |
| TGAATTGAATAGTTACTTTC+CGG | 0.283666 | 5:-213164 | None:intergenic |
| CTCACGAAGGCCTTCAATTA+GGG | 0.298122 | 5:-213086 | None:intergenic |
| ATGAACTTCTCTTGAAATAC+AGG | 0.329350 | 5:-213058 | None:intergenic |
| GGCCAACCTCTCGAGGTTAC+TGG | 0.375943 | 5:+212733 | MsG0580024043.01.T01:CDS |
| GTGAGATCAATGTTGTCGTT+TGG | 0.408755 | 5:+213104 | MsG0580024043.01.T01:CDS |
| TTCATACTTCCCCTAATTGA+AGG | 0.420730 | 5:+213076 | MsG0580024043.01.T01:CDS |
| GCATGTTCTTGTTCGGAACT+TGG | 0.446926 | 5:-212921 | None:intergenic |
| CGAAATTGAAAGACACTGAA+TGG | 0.450034 | 5:+212855 | MsG0580024043.01.T01:CDS |
| AACCAGTGCATGTTCTTGTT+CGG | 0.457517 | 5:-212928 | None:intergenic |
| TCACTCACGATGATGATGTC+TGG | 0.459098 | 5:+212703 | MsG0580024043.01.T01:exon |
| CACTCACGATGATGATGTCT+GGG | 0.514047 | 5:+212704 | MsG0580024043.01.T01:exon |
| ATGATGATGTCTGGGATTCA+AGG | 0.520106 | 5:+212712 | MsG0580024043.01.T01:CDS |
| TACCAGTAACCTCGAGAGGT+TGG | 0.560064 | 5:-212735 | None:intergenic |
| TTCCGAACAAGAACATGCAC+TGG | 0.585438 | 5:+212926 | MsG0580024043.01.T01:CDS |
| AGCGTATTCGATGCAAACGT+AGG | 0.585596 | 5:-212894 | None:intergenic |
| GATAAAATCATCGAAGGAGA+CGG | 0.590599 | 5:-213137 | None:intergenic |
| TCACGAAGGCCTTCAATTAG+GGG | 0.593261 | 5:-213085 | None:intergenic |
| TGTTCTTGTTCGGAACTTGG+TGG | 0.595740 | 5:-212918 | None:intergenic |
| TGAACTTCTCTTGAAATACA+GGG | 0.608951 | 5:-213057 | None:intergenic |
| GCGTATTCGATGCAAACGTA+GGG | 0.609000 | 5:-212893 | None:intergenic |
| ATCTTACCAGTAACCTCGAG+AGG | 0.614688 | 5:-212739 | None:intergenic |
| GATTCAAGGCCAACCTCTCG+AGG | 0.618619 | 5:+212726 | MsG0580024043.01.T01:CDS |
| GGTGCCGATAAAATCATCGA+AGG | 0.667788 | 5:-213143 | None:intergenic |
| CGACAACATTGATCTCACGA+AGG | 0.730875 | 5:-213099 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACGACTACAATAACAGAA+AGG | - | Chr5:212829-212848 | None:intergenic | 30.0% | |
| AAAGAGTCAACGATTCAAAT+AGG | - | Chr5:213011-213030 | None:intergenic | 30.0% | |
| AAGAGTCAACGATTCAAATA+GGG | - | Chr5:213010-213029 | None:intergenic | 30.0% | |
| ATGAACTTCTCTTGAAATAC+AGG | - | Chr5:213061-213080 | None:intergenic | 30.0% | |
| TGAACTTCTCTTGAAATACA+GGG | - | Chr5:213060-213079 | None:intergenic | 30.0% | |
| CGAAATTGAAAGACACTGAA+TGG | + | Chr5:212855-212874 | MsG0580024043.01.T01:CDS | 35.0% | |
| CTCTTTCTTTCAATTCCAGA+CGG | + | Chr5:213025-213044 | MsG0580024043.01.T01:intron | 35.0% | |
| GATAAAATCATCGAAGGAGA+CGG | - | Chr5:213140-213159 | None:intergenic | 35.0% | |
| TTCATACTTCCCCTAATTGA+AGG | + | Chr5:213076-213095 | MsG0580024043.01.T01:CDS | 35.0% | |
| ! | GTCTCCTTCGATGATTTTAT+CGG | + | Chr5:213139-213158 | MsG0580024043.01.T01:CDS | 35.0% |
| AACCAGTGCATGTTCTTGTT+CGG | - | Chr5:212931-212950 | None:intergenic | 40.0% | |
| GTGAGATCAATGTTGTCGTT+TGG | + | Chr5:213104-213123 | MsG0580024043.01.T01:CDS | 40.0% | |
| TTTCTTTCAATTCCAGACGG+CGG | + | Chr5:213028-213047 | MsG0580024043.01.T01:intron | 40.0% | |
| ! | ATGATGATGTCTGGGATTCA+AGG | + | Chr5:212712-212731 | MsG0580024043.01.T01:CDS | 40.0% |
| ! | TTCGATGATTTTATCGGCAC+CGG | + | Chr5:213145-213164 | MsG0580024043.01.T01:CDS | 40.0% |
| AGCGTATTCGATGCAAACGT+AGG | - | Chr5:212897-212916 | None:intergenic | 45.0% | |
| ATCTTACCAGTAACCTCGAG+AGG | - | Chr5:212742-212761 | None:intergenic | 45.0% | |
| CGACAACATTGATCTCACGA+AGG | - | Chr5:213102-213121 | None:intergenic | 45.0% | |
| CTCACGAAGGCCTTCAATTA+GGG | - | Chr5:213089-213108 | None:intergenic | 45.0% | |
| GCATGTTCTTGTTCGGAACT+TGG | - | Chr5:212924-212943 | None:intergenic | 45.0% | |
| GCGTATTCGATGCAAACGTA+GGG | - | Chr5:212896-212915 | None:intergenic | 45.0% | |
| TCACGAAGGCCTTCAATTAG+GGG | - | Chr5:213088-213107 | None:intergenic | 45.0% | |
| TCTCACGAAGGCCTTCAATT+AGG | - | Chr5:213090-213109 | None:intergenic | 45.0% | |
| TGTTCTTGTTCGGAACTTGG+TGG | - | Chr5:212921-212940 | None:intergenic | 45.0% | |
| TTCCGAACAAGAACATGCAC+TGG | + | Chr5:212926-212945 | MsG0580024043.01.T01:CDS | 45.0% | |
| ! | CACTCACGATGATGATGTCT+GGG | + | Chr5:212704-212723 | MsG0580024043.01.T01:exon | 45.0% |
| ! | TCACTCACGATGATGATGTC+TGG | + | Chr5:212703-212722 | MsG0580024043.01.T01:exon | 45.0% |
| !! | GGTGCCGATAAAATCATCGA+AGG | - | Chr5:213146-213165 | None:intergenic | 45.0% |
| TACCAGTAACCTCGAGAGGT+TGG | - | Chr5:212738-212757 | None:intergenic | 50.0% | |
| GATTCAAGGCCAACCTCTCG+AGG | + | Chr5:212726-212745 | MsG0580024043.01.T01:CDS | 55.0% | |
| !!! | ACAGGGTTTTTTCCGCCGTC+TGG | - | Chr5:213043-213062 | None:intergenic | 55.0% |
| GGCCAACCTCTCGAGGTTAC+TGG | + | Chr5:212733-212752 | MsG0580024043.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 212666 | 213174 | 212666 | ID=MsG0580024043.01;Name=MsG0580024043.01 |
| Chr5 | mRNA | 212666 | 213174 | 212666 | ID=MsG0580024043.01.T01;Parent=MsG0580024043.01;Name=MsG0580024043.01.T01;_AED=0.43;_eAED=0.44;_QI=46|1|0.66|1|0|0|3|0|92 |
| Chr5 | exon | 212666 | 212754 | 212666 | ID=MsG0580024043.01.T01:exon:22986;Parent=MsG0580024043.01.T01 |
| Chr5 | exon | 212843 | 212947 | 212843 | ID=MsG0580024043.01.T01:exon:22987;Parent=MsG0580024043.01.T01 |
| Chr5 | exon | 213044 | 213174 | 213044 | ID=MsG0580024043.01.T01:exon:22988;Parent=MsG0580024043.01.T01 |
| Chr5 | five_prime_UTR | 212666 | 212711 | 212666 | ID=MsG0580024043.01.T01:five_prime_utr;Parent=MsG0580024043.01.T01 |
| Chr5 | CDS | 212712 | 212754 | 212712 | ID=MsG0580024043.01.T01:cds;Parent=MsG0580024043.01.T01 |
| Chr5 | CDS | 212843 | 212947 | 212843 | ID=MsG0580024043.01.T01:cds;Parent=MsG0580024043.01.T01 |
| Chr5 | CDS | 213044 | 213174 | 213044 | ID=MsG0580024043.01.T01:cds;Parent=MsG0580024043.01.T01 |
| Gene Sequence |
| Protein sequence |
