AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004941.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004941.01.T01 MTR_1g090060 83.962 106 17 0 1 106 67 172 1.03e-61 187
MsG0180004941.01.T01 MTR_1g090100 83.333 102 17 0 1 102 66 167 1.42e-57 177
MsG0180004941.01.T01 MTR_1g090090 77.451 102 23 0 1 102 67 168 3.78e-54 168
MsG0180004941.01.T01 MTR_1g090070 74.757 103 25 1 1 102 73 175 7.59e-51 160
MsG0180004941.01.T01 MTR_4g059730 60.204 98 39 0 1 98 68 165 3.03e-39 130
MsG0180004941.01.T01 MTR_2g070140 46.316 95 48 2 1 94 52 144 1.38e-22 87.4
MsG0180004941.01.T01 MTR_2g070150 42.241 116 55 3 1 106 68 181 2.09e-22 87.0
MsG0180004941.01.T01 MTR_2g070200 45.098 102 52 3 1 100 68 167 3.36e-21 84.3
MsG0180004941.01.T01 MTR_2g070210 40.594 101 57 2 1 100 70 168 4.67e-21 84.0
MsG0180004941.01.T01 MTR_7g065710 46.667 90 44 2 2 90 69 155 5.81e-21 83.6
MsG0180004941.01.T01 MTR_3g099757 41.000 100 57 1 1 100 69 166 1.39e-20 82.8
MsG0180004941.01.T01 MTR_2g070130 43.478 92 49 2 1 91 68 157 2.00e-20 82.0
MsG0180004941.01.T01 MTR_2g070120 43.333 90 48 2 3 91 70 157 2.24e-20 82.0
MsG0180004941.01.T01 MTR_3g467430 41.176 102 57 2 3 103 75 174 4.50e-20 81.3
MsG0180004941.01.T01 MTR_3g467420 42.553 94 51 2 3 95 75 166 1.01e-19 80.5
MsG0180004941.01.T01 MTR_7g065230 40.566 106 58 3 1 105 71 172 1.24e-19 80.1
MsG0180004941.01.T01 MTR_7g065630 42.857 105 56 2 2 105 90 191 1.27e-19 80.5
MsG0180004941.01.T01 MTR_7g065600 42.857 98 51 3 1 97 71 164 2.19e-19 79.7
MsG0180004941.01.T01 MTR_2g070180 43.478 92 49 2 1 91 68 157 2.68e-19 79.3
MsG0180004941.01.T01 MTR_1g090150 45.161 93 48 2 1 92 67 157 2.98e-19 79.3
MsG0180004941.01.T01 MTR_7g065720 44.565 92 46 2 1 90 71 159 3.03e-19 79.3
MsG0180004941.01.T01 MTR_4g019780 46.939 98 47 4 1 97 72 165 4.06e-19 78.6
MsG0180004941.01.T01 MTR_1g115195 40.594 101 57 2 1 99 67 166 1.55e-18 77.4
MsG0180004941.01.T01 MTR_8g087410 41.000 100 57 1 1 100 70 167 1.97e-18 77.0
MsG0180004941.01.T01 MTR_7g065660 40.816 98 53 3 1 97 71 164 2.19e-18 77.0
MsG0180004941.01.T01 MTR_7g065270 41.758 91 49 2 1 90 71 158 3.35e-18 76.3
MsG0180004941.01.T01 MTR_5g040430 39.583 96 54 2 2 93 72 167 4.83e-18 75.9
MsG0180004941.01.T01 MTR_8g056940 40.566 106 59 4 1 104 68 171 6.67e-18 75.5
MsG0180004941.01.T01 MTR_4g124130 40.816 98 55 2 1 97 69 164 8.55e-18 75.1
MsG0180004941.01.T01 MTR_4g019790 38.776 98 55 3 1 97 71 164 1.25e-17 75.1
MsG0180004941.01.T01 MTR_2g070060 43.617 94 50 2 2 94 69 160 1.28e-17 74.7
MsG0180004941.01.T01 MTR_5g037380 40.187 107 58 3 2 102 70 176 2.21e-17 74.3
MsG0180004941.01.T01 MTR_8g061950 41.905 105 55 3 1 100 71 174 2.65e-17 74.3
MsG0180004941.01.T01 MTR_6g080440 41.935 93 50 3 1 92 67 156 4.14e-17 73.6
MsG0180004941.01.T01 MTR_7g065290 40.659 91 50 2 1 90 71 158 5.59e-17 73.2
MsG0180004941.01.T01 MTR_7g065740 39.394 99 54 3 1 97 71 165 6.21e-17 73.2
MsG0180004941.01.T01 MTR_7g065590 40.404 99 52 4 1 97 71 164 6.76e-17 73.2
MsG0180004941.01.T01 MTR_7g065265 42.857 91 48 2 1 90 71 158 2.06e-16 71.6
MsG0180004941.01.T01 MTR_7g065750 38.384 99 55 3 1 97 71 165 4.33e-16 70.9
MsG0180004941.01.T01 MTR_5g076900 39.773 88 51 1 2 89 72 157 1.64e-15 69.3
MsG0180004941.01.T01 MTR_7g065680 46.667 90 44 2 2 90 72 158 2.94e-14 65.9
MsG0180004941.01.T01 MTR_7g065700 44.444 99 50 3 2 99 72 166 3.05e-14 65.9
MsG0180004941.01.T01 MTR_8g087425 40.217 92 53 1 1 92 69 158 3.10e-14 65.9
MsG0180004941.01.T01 MTR_7g065640 39.437 71 42 1 1 70 71 141 1.58e-13 63.9
MsG0180004941.01.T01 MTR_2g070070 41.489 94 52 2 2 94 69 160 4.92e-13 62.8
MsG0180004941.01.T01 MTR_7g065260 38.614 101 51 4 1 97 78 171 3.23e-12 60.8
MsG0180004941.01.T01 MTR_2g070110 41.489 94 51 3 2 94 69 159 3.83e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004941.01.T01 AT1G78340 41.748 103 57 2 1 102 68 168 1.05e-21 85.5
MsG0180004941.01.T01 AT1G17180 41.584 101 56 2 1 100 68 166 3.85e-20 81.6
MsG0180004941.01.T01 AT2G29470 38.542 96 57 1 2 97 73 166 5.27e-19 78.6
MsG0180004941.01.T01 AT1G78370 36.364 99 60 2 3 100 70 166 2.47e-18 76.6
MsG0180004941.01.T01 AT1G10370 40.367 109 60 3 2 105 70 178 3.28e-18 76.6
MsG0180004941.01.T01 AT1G17170 41.584 101 55 3 1 100 68 165 4.55e-18 75.9
MsG0180004941.01.T01 AT1G78380 41.053 95 53 2 1 94 68 160 5.77e-18 75.9
MsG0180004941.01.T01 AT1G59670 40.952 105 57 3 3 102 73 177 8.57e-18 75.5
MsG0180004941.01.T01 AT2G29480 38.542 96 57 1 2 97 72 165 1.25e-17 75.1
MsG0180004941.01.T01 AT2G29460 37.500 96 58 1 2 97 72 165 1.31e-17 75.1
MsG0180004941.01.T01 AT2G29420 39.175 97 56 2 1 97 73 166 2.19e-17 74.3
MsG0180004941.01.T01 AT1G10360 43.000 100 50 4 2 95 70 168 2.33e-17 74.3
MsG0180004941.01.T01 AT1G53680 39.252 107 60 3 1 104 71 175 4.18e-17 73.6
MsG0180004941.01.T01 AT1G78320 40.000 95 54 2 1 94 68 160 4.27e-17 73.6
MsG0180004941.01.T01 AT2G29490 38.542 96 57 1 2 97 72 165 5.86e-17 73.2
MsG0180004941.01.T01 AT1G78360 38.947 95 55 2 1 94 69 161 6.86e-17 72.8
MsG0180004941.01.T01 AT3G09270 42.857 91 49 2 1 90 71 159 1.54e-16 72.0
MsG0180004941.01.T01 AT2G29450 38.000 100 53 3 2 97 71 165 2.42e-16 71.6
MsG0180004941.01.T01 AT2G29440 39.394 99 52 3 2 97 71 164 3.20e-16 71.2
MsG0180004941.01.T01 AT1G59700 39.048 105 59 3 3 102 73 177 6.07e-16 70.9
MsG0180004941.01.T01 AT3G43800 38.542 96 55 2 2 94 71 165 1.62e-15 69.3
MsG0180004941.01.T01 AT1G17190 32.673 101 65 2 1 100 69 167 2.50e-15 68.9
MsG0180004941.01.T01 AT1G27140 40.196 102 57 3 3 100 75 176 2.84e-15 68.9
MsG0180004941.01.T01 AT1G27130 38.235 102 59 3 3 100 74 175 4.46e-15 68.2
MsG0180004941.01.T01 AT1G69920 37.255 102 60 3 3 100 102 203 2.71e-13 63.9
MsG0180004941.01.T01 AT1G74590 37.143 70 43 1 1 69 71 140 2.36e-12 61.2
MsG0180004941.01.T01 AT5G62480 32.353 102 62 2 1 95 72 173 6.24e-12 60.1
MsG0180004941.01.T01 AT1G78320 37.975 79 47 1 16 94 57 133 2.84e-11 57.8

Find 19 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGAGCTCAAGCCCGATTT+TGG 0.174441 1:-84713525 MsG0180004941.01.T01:CDS
AGAAGGCCAGAGAGAATTTA+TGG 0.204560 1:-84712778 MsG0180004941.01.T01:CDS
GCACTCCTCTATAGCAAAAT+TGG 0.275055 1:-84712812 MsG0180004941.01.T01:CDS
AGCTATGTCTACAATGTTAA+TGG 0.297246 1:+84712691 None:intergenic
CAACATATTTAACCCAAAAT+CGG 0.352973 1:+84713512 None:intergenic
TCGGGCTTGAGCTCTGTCAT+AGG 0.384467 1:+84713531 None:intergenic
GGTTAAATATGTTGATGAAT+TGG 0.403400 1:-84713503 MsG0180004941.01.T01:intron
AGTTATTGAAGATAAGTGTT+TGG 0.410970 1:-84712744 MsG0180004941.01.T01:CDS
AACATATTTAACCCAAAATC+GGG 0.426033 1:+84713513 None:intergenic
AACATTGTAGACATAGCTCT+TGG 0.450264 1:-84712686 MsG0180004941.01.T01:CDS
GCTCTGTCATAGGGATCAAC+AGG 0.468867 1:+84713541 None:intergenic
AATGTATTCAACAATGATCA+TGG 0.484642 1:+84713594 None:intergenic
GAAGGCCAGAGAGAATTTAT+GGG 0.489428 1:-84712777 MsG0180004941.01.T01:CDS
TCGCTCCCATAAATTCTCTC+TGG 0.520342 1:+84712772 None:intergenic
CGGGCTTGAGCTCTGTCATA+GGG 0.535527 1:+84713532 None:intergenic
GATGTGGTCACACAACTCAT+TGG 0.539768 1:-84713566 MsG0180004941.01.T01:CDS
TTTACAGATTTCGGCAATTG+CGG 0.548298 1:-84712834 MsG0180004941.01.T01:intron
TTGAATACATTGATGAGATG+TGG 0.630251 1:-84713582 MsG0180004941.01.T01:CDS
AATTGGTGAAGAGCGAGAGA+AGG 0.634346 1:-84712795 MsG0180004941.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTAATTTATCTTTATGAA+TGG - Chr1:84713111-84713130 MsG0180004941.01.T01:intron 15.0%
!! TAAATTAAATTAAATCCTCA+TGG + Chr1:84712891-84712910 None:intergenic 15.0%
!! TTAATCTTAAGTAATCAAAA+AGG + Chr1:84713232-84713251 None:intergenic 15.0%
!!! ATCTTTTATCAAATTTATTG+AGG - Chr1:84713569-84713588 MsG0180004941.01.T01:CDS 15.0%
!!! ATGATCAAAAGAAATTTTAT+GGG - Chr1:84713511-84713530 MsG0180004941.01.T01:CDS 15.0%
!!! GAGGATTTAATTTAATTTAT+AGG - Chr1:84712892-84712911 MsG0180004941.01.T01:intron 15.0%
!! AAATTAAGTTAAAGGATTCA+TGG - Chr1:84713262-84713281 MsG0180004941.01.T01:intron 20.0%
!! AAGAATGAAAGTTGAATTAA+CGG + Chr1:84712928-84712947 None:intergenic 20.0%
!! TGAAACATTCTTAATAGTAT+AGG + Chr1:84712996-84713015 None:intergenic 20.0%
!!! GATGATCAAAAGAAATTTTA+TGG - Chr1:84713510-84713529 MsG0180004941.01.T01:CDS 20.0%
!!! TGATCAAAAGAAATTTTATG+GGG - Chr1:84713512-84713531 MsG0180004941.01.T01:CDS 20.0%
! AACATATTTAACCCAAAATC+GGG + Chr1:84712722-84712741 None:intergenic 25.0%
! AATCTATAATTTACTTTCCC+TGG - Chr1:84713303-84713322 MsG0180004941.01.T01:intron 25.0%
! AGTTATTGAAGATAAGTGTT+TGG - Chr1:84713488-84713507 MsG0180004941.01.T01:intron 25.0%
! CAACATATTTAACCCAAAAT+CGG + Chr1:84712723-84712742 None:intergenic 25.0%
! GGATCAATATTTACAGATTT+CGG - Chr1:84713389-84713408 MsG0180004941.01.T01:intron 25.0%
! GTAATAATTATAAGACACCA+GGG + Chr1:84713323-84713342 None:intergenic 25.0%
! TGATTCAGACTAACAATATT+TGG - Chr1:84713368-84713387 MsG0180004941.01.T01:intron 25.0%
! TTTATGTTCAAAAACGTCAA+CGG + Chr1:84713180-84713199 None:intergenic 25.0%
!! GAATGGTTAAGTATAACTTT+TGG - Chr1:84713128-84713147 MsG0180004941.01.T01:intron 25.0%
!! GATCAAAAGAAATTTTATGG+GGG - Chr1:84713513-84713532 MsG0180004941.01.T01:CDS 25.0%
!! GGTTAAATATGTTGATGAAT+TGG - Chr1:84712729-84712748 MsG0180004941.01.T01:CDS 25.0%
AACTTTGCCTATAAAATGAG+TGG + Chr1:84712865-84712884 None:intergenic 30.0%
AGCTATGTCTACAATGTTAA+TGG + Chr1:84713544-84713563 None:intergenic 30.0%
ATGTATTGTGCAAGTACAAT+TGG + Chr1:84712839-84712858 None:intergenic 30.0%
GGTAATAATTATAAGACACC+AGG + Chr1:84713324-84713343 None:intergenic 30.0%
TGAGATGGTTAGAACATATT+AGG + Chr1:84713345-84713364 None:intergenic 30.0%
TTGAATACATTGATGAGATG+TGG - Chr1:84712650-84712669 MsG0180004941.01.T01:CDS 30.0%
! ACATGTTCCACTCATTTTAT+AGG - Chr1:84712855-84712874 MsG0180004941.01.T01:intron 30.0%
!!! TTCATTTTTTCGTGTCAAGT+TGG + Chr1:84713206-84713225 None:intergenic 30.0%
AACATTGTAGACATAGCTCT+TGG - Chr1:84713546-84713565 MsG0180004941.01.T01:CDS 35.0%
CAATTGGATTATGATCACGA+TGG + Chr1:84712823-84712842 None:intergenic 35.0%
TAGTCTGAATCAAGATGAGA+TGG + Chr1:84713360-84713379 None:intergenic 35.0%
TTTACAGATTTCGGCAATTG+CGG - Chr1:84713398-84713417 MsG0180004941.01.T01:intron 35.0%
!! CTTCACCAATTTTGCTATAG+AGG + Chr1:84713428-84713447 None:intergenic 35.0%
AGAAGGCCAGAGAGAATTTA+TGG - Chr1:84713454-84713473 MsG0180004941.01.T01:intron 40.0%
ATAGGCAAAGTTACACCATG+AGG - Chr1:84712873-84712892 MsG0180004941.01.T01:intron 40.0%
GAAGGCCAGAGAGAATTTAT+GGG - Chr1:84713455-84713474 MsG0180004941.01.T01:intron 40.0%
GCACTCCTCTATAGCAAAAT+TGG - Chr1:84713420-84713439 MsG0180004941.01.T01:intron 40.0%
AATTGGTGAAGAGCGAGAGA+AGG - Chr1:84713437-84713456 MsG0180004941.01.T01:intron 45.0%
GATGTGGTCACACAACTCAT+TGG - Chr1:84712666-84712685 MsG0180004941.01.T01:CDS 45.0%
TCGCTCCCATAAATTCTCTC+TGG + Chr1:84713463-84713482 None:intergenic 45.0%
!! TATAAAAAAAATTAAGTTAA+AGG - Chr1:84713254-84713273 MsG0180004941.01.T01:intron 5.0%
GCTCTGTCATAGGGATCAAC+AGG + Chr1:84712694-84712713 None:intergenic 50.0%
! ACAGAGCTCAAGCCCGATTT+TGG - Chr1:84712707-84712726 MsG0180004941.01.T01:CDS 50.0%
! CAGAGCTCAAGCCCGATTTT+GGG - Chr1:84712708-84712727 MsG0180004941.01.T01:CDS 50.0%
CGGGCTTGAGCTCTGTCATA+GGG + Chr1:84712703-84712722 None:intergenic 55.0%
TCGGGCTTGAGCTCTGTCAT+AGG + Chr1:84712704-84712723 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 84712640 84713614 84712640 ID=MsG0180004941.01;Name=MsG0180004941.01
Chr1 mRNA 84712640 84713614 84712640 ID=MsG0180004941.01.T01;Parent=MsG0180004941.01;Name=MsG0180004941.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|106
Chr1 exon 84713504 84713614 84713504 ID=MsG0180004941.01.T01:exon:9428;Parent=MsG0180004941.01.T01
Chr1 exon 84712640 84712849 84712640 ID=MsG0180004941.01.T01:exon:9427;Parent=MsG0180004941.01.T01
Chr1 CDS 84713504 84713614 84713504 ID=MsG0180004941.01.T01:cds;Parent=MsG0180004941.01.T01
Chr1 CDS 84712640 84712849 84712640 ID=MsG0180004941.01.T01:cds;Parent=MsG0180004941.01.T01
Gene Sequence

>MsG0180004941.01.T01

ATGATCATTGTTGAATACATTGATGAGATGTGGTCACACAACTCATTGGTTCCTGTTGATCCCTATGACAGAGCTCAAGCCCGATTTTGGGTTAAATATGTTGATGAATTGATTTCGGCAATTGCGGCACTCCTCTATAGCAAAATTGGTGAAGAGCGAGAGAAGGCCAGAGAGAATTTATGGGAGCGACTCAGAGTTATTGAAGATAAGTGTTTGGATGATCAAAAGAAATTTTATGGGGGAGACACCATTAACATTGTAGACATAGCTCTTGGATCTTTTATCAAATTTATTGAGGTTCAGAAGATATGTTTGAAGTGA

Protein sequence

>MsG0180004941.01.T01

MIIVEYIDEMWSHNSLVPVDPYDRAQARFWVKYVDELISAIAALLYSKIGEEREKARENLWERLRVIEDKCLDDQKKFYGGDTINIVDIALGSFIKFIEVQKICLK*