AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039978.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039978.01.T01 MTR_7g082760 97.500 120 3 0 1 120 7 126 3.25e-83 239
MsG0780039978.01.T01 MTR_7g082750 50.376 133 47 5 1 120 3 129 5.84e-35 117
MsG0780039978.01.T01 MTR_7g082740 52.756 127 45 4 9 120 4 130 6.42e-34 114
MsG0780039978.01.T01 MTR_7g082700 52.756 127 45 4 9 120 4 130 1.39e-33 114
MsG0780039978.01.T01 MTR_7g082730 55.200 125 41 5 10 120 5 128 5.44e-33 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTTGTCCACATCTTTAT+TGG 0.171024 7:-71383402 None:intergenic
CCGTAAGTACCTCGTTGGTT+TGG 0.301020 7:-71383250 None:intergenic
ACTTATATTTGAGATTTCTA+AGG 0.310038 7:+71383539 MsG0780039978.01.T01:CDS
AGAAGGATTAAACTATTTCA+TGG 0.325765 7:-71383589 None:intergenic
CAAGGTTAACATGGGCTATT+AGG 0.336227 7:+71383376 MsG0780039978.01.T01:CDS
ATGAAAAGTGATACCATCTT+TGG 0.337936 7:+71383459 MsG0780039978.01.T01:CDS
CGTAAGTACCTCGTTGGTTT+GGG 0.339937 7:-71383249 None:intergenic
TAAGGATCGTGTAGTTTCTA+AGG 0.360505 7:+71383485 MsG0780039978.01.T01:CDS
ATTTGAGATTTCTAAGGCCT+TGG 0.387808 7:+71383545 MsG0780039978.01.T01:CDS
GAAGGAAGACCAAGGTTCTA+AGG 0.400859 7:+71383319 MsG0780039978.01.T01:CDS
TGATACCATCTTTGGAACTA+AGG 0.464934 7:+71383467 MsG0780039978.01.T01:CDS
TCTTCTCAACCTTAGAACCT+TGG 0.471764 7:-71383328 None:intergenic
AGGTTCTAAGGTTGAGAAGA+AGG 0.473602 7:+71383331 MsG0780039978.01.T01:CDS
CCAAACCAACGAGGTACTTA+CGG 0.478482 7:+71383250 MsG0780039978.01.T01:CDS
CTTCCTTCCATCACCGGTGA+TGG 0.490534 7:-71383304 None:intergenic
CGCCTCCGTAAGTACCTCGT+TGG 0.498443 7:-71383255 None:intergenic
CTTGGTCTTCCTTCCATCAC+CGG 0.498548 7:-71383310 None:intergenic
GAGTAGTTCCAAGGTTAACA+TGG 0.518857 7:+71383367 MsG0780039978.01.T01:CDS
GAGGATGCCATCACCGGTGA+TGG 0.521516 7:+71383297 MsG0780039978.01.T01:CDS
CTAATAGCCCATGTTAACCT+TGG 0.534159 7:-71383375 None:intergenic
CTCGACGAGGATGCCATCAC+CGG 0.557338 7:+71383291 MsG0780039978.01.T01:CDS
TAAGGTTGAGAAGAAGGCAG+TGG 0.557373 7:+71383337 MsG0780039978.01.T01:CDS
ACGATCCTTAGTTCCAAAGA+TGG 0.563624 7:-71383472 None:intergenic
ATGCCATCACCGGTGATGGA+AGG 0.564522 7:+71383301 MsG0780039978.01.T01:CDS
GGTTGAGAAGAAGGCAGTGG+AGG 0.585451 7:+71383340 MsG0780039978.01.T01:CDS
GGAGGATAAGAGTAGTTCCA+AGG 0.595694 7:+71383358 MsG0780039978.01.T01:CDS
CAACGAGGTACTTACGGAGG+CGG 0.621368 7:+71383256 MsG0780039978.01.T01:CDS
AGTAGTTCCAAGGTTAACAT+GGG 0.641526 7:+71383368 MsG0780039978.01.T01:CDS
TAATTCATGAGAAGCAACCA+AGG 0.645785 7:-71383562 None:intergenic
TTAGGTCACCAATAAAGATG+TGG 0.652661 7:+71383394 MsG0780039978.01.T01:CDS
GCGATATCAGCGACTCGACG+AGG 0.678608 7:+71383278 MsG0780039978.01.T01:CDS
CGGTGATGGAAGGAAGACCA+AGG 0.711559 7:+71383311 MsG0780039978.01.T01:CDS
AACCAACGAGGTACTTACGG+AGG 0.715584 7:+71383253 MsG0780039978.01.T01:CDS
CACTGATACCCAAACCAACG+AGG 0.721556 7:+71383241 MsG0780039978.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTATATTTGAGATTTCTA+AGG + Chr7:71383539-71383558 MsG0780039978.01.T01:CDS 20.0%
ATGAAAAGTGATACCATCTT+TGG + Chr7:71383459-71383478 MsG0780039978.01.T01:CDS 30.0%
!! AAAGATGGTATCACTTTTCA+TGG - Chr7:71383460-71383479 None:intergenic 30.0%
AGTAGTTCCAAGGTTAACAT+GGG + Chr7:71383368-71383387 MsG0780039978.01.T01:CDS 35.0%
TAAGGATCGTGTAGTTTCTA+AGG + Chr7:71383485-71383504 MsG0780039978.01.T01:CDS 35.0%
TAATTCATGAGAAGCAACCA+AGG - Chr7:71383565-71383584 None:intergenic 35.0%
TTAGGTCACCAATAAAGATG+TGG + Chr7:71383394-71383413 MsG0780039978.01.T01:CDS 35.0%
! AGCTTTGTCCACATCTTTAT+TGG - Chr7:71383405-71383424 None:intergenic 35.0%
! TTATTCTCGTGATGCTTTTG+AGG + Chr7:71383512-71383531 MsG0780039978.01.T01:CDS 35.0%
!! ATTTGAGATTTCTAAGGCCT+TGG + Chr7:71383545-71383564 MsG0780039978.01.T01:CDS 35.0%
!! TGATACCATCTTTGGAACTA+AGG + Chr7:71383467-71383486 MsG0780039978.01.T01:CDS 35.0%
ACGATCCTTAGTTCCAAAGA+TGG - Chr7:71383475-71383494 None:intergenic 40.0%
CTAATAGCCCATGTTAACCT+TGG - Chr7:71383378-71383397 None:intergenic 40.0%
GAGTAGTTCCAAGGTTAACA+TGG + Chr7:71383367-71383386 MsG0780039978.01.T01:CDS 40.0%
TCTTCTCAACCTTAGAACCT+TGG - Chr7:71383331-71383350 None:intergenic 40.0%
! CAAGGTTAACATGGGCTATT+AGG + Chr7:71383376-71383395 MsG0780039978.01.T01:CDS 40.0%
!! AGGTTCTAAGGTTGAGAAGA+AGG + Chr7:71383331-71383350 MsG0780039978.01.T01:CDS 40.0%
GAAGGAAGACCAAGGTTCTA+AGG + Chr7:71383319-71383338 MsG0780039978.01.T01:CDS 45.0%
GGAGGATAAGAGTAGTTCCA+AGG + Chr7:71383358-71383377 MsG0780039978.01.T01:CDS 45.0%
TAAGGTTGAGAAGAAGGCAG+TGG + Chr7:71383337-71383356 MsG0780039978.01.T01:CDS 45.0%
! CCAAACCAACGAGGTACTTA+CGG + Chr7:71383250-71383269 MsG0780039978.01.T01:CDS 45.0%
!! CGTAAGTACCTCGTTGGTTT+GGG - Chr7:71383252-71383271 None:intergenic 45.0%
CACTGATACCCAAACCAACG+AGG + Chr7:71383241-71383260 MsG0780039978.01.T01:CDS 50.0%
! AACCAACGAGGTACTTACGG+AGG + Chr7:71383253-71383272 MsG0780039978.01.T01:CDS 50.0%
! CCGTAAGTACCTCGTTGGTT+TGG - Chr7:71383253-71383272 None:intergenic 50.0%
! CTTGGTCTTCCTTCCATCAC+CGG - Chr7:71383313-71383332 None:intergenic 50.0%
CTTCCTTCCATCACCGGTGA+TGG - Chr7:71383307-71383326 None:intergenic 55.0%
GGTTGAGAAGAAGGCAGTGG+AGG + Chr7:71383340-71383359 MsG0780039978.01.T01:CDS 55.0%
! CAACGAGGTACTTACGGAGG+CGG + Chr7:71383256-71383275 MsG0780039978.01.T01:CDS 55.0%
!! ATGCCATCACCGGTGATGGA+AGG + Chr7:71383301-71383320 MsG0780039978.01.T01:CDS 55.0%
!! CGGTGATGGAAGGAAGACCA+AGG + Chr7:71383311-71383330 MsG0780039978.01.T01:CDS 55.0%
CGCCTCCGTAAGTACCTCGT+TGG - Chr7:71383258-71383277 None:intergenic 60.0%
CTCGACGAGGATGCCATCAC+CGG + Chr7:71383291-71383310 MsG0780039978.01.T01:CDS 60.0%
GAGGATGCCATCACCGGTGA+TGG + Chr7:71383297-71383316 MsG0780039978.01.T01:CDS 60.0%
GCGATATCAGCGACTCGACG+AGG + Chr7:71383278-71383297 MsG0780039978.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 71383234 71383599 71383234 ID=MsG0780039978.01;Name=MsG0780039978.01
Chr7 mRNA 71383234 71383599 71383234 ID=MsG0780039978.01.T01;Parent=MsG0780039978.01;Name=MsG0780039978.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|121
Chr7 exon 71383234 71383599 71383234 ID=MsG0780039978.01.T01:exon:11769;Parent=MsG0780039978.01.T01
Chr7 CDS 71383234 71383599 71383234 ID=MsG0780039978.01.T01:cds;Parent=MsG0780039978.01.T01
Gene Sequence

>MsG0780039978.01.T01

ATGGAGTCACTGATACCCAAACCAACGAGGTACTTACGGAGGCGGCGATATCAGCGACTCGACGAGGATGCCATCACCGGTGATGGAAGGAAGACCAAGGTTCTAAGGTTGAGAAGAAGGCAGTGGAGGATAAGAGTAGTTCCAAGGTTAACATGGGCTATTAGGTCACCAATAAAGATGTGGACAAAGCTTAAGAACACTTATGAGAAATTTATGTTGAAGTCCATGAAAAGTGATACCATCTTTGGAACTAAGGATCGTGTAGTTTCTAAGGAAAATTATTCTCGTGATGCTTTTGAGGCTAGACTTATATTTGAGATTTCTAAGGCCTTGGTTGCTTCTCATGAATTAAATTCCATGAAATAG

Protein sequence

>MsG0780039978.01.T01

MESLIPKPTRYLRRRRYQRLDEDAITGDGRKTKVLRLRRRQWRIRVVPRLTWAIRSPIKMWTKLKNTYEKFMLKSMKSDTIFGTKDRVVSKENYSRDAFEARLIFEISKALVASHELNSMK*