AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024219.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024219.01.T01 MTR_5g007713 98.419 253 4 0 1 253 137 389 0.0 518
MsG0580024219.01.T01 MTR_5g007717 98.419 253 4 0 1 253 137 389 0.0 517
MsG0580024219.01.T01 MTR_1g098140 95.652 253 11 0 1 253 137 389 0.0 506
MsG0580024219.01.T01 MTR_1g097935 95.257 253 12 0 1 253 137 389 0.0 502
MsG0580024219.01.T01 MTR_1g097910 94.862 253 13 0 1 253 137 389 0.0 502
MsG0580024219.01.T01 MTR_5g007730 94.466 253 14 0 1 253 137 389 2.58e-180 501
MsG0580024219.01.T01 MTR_7g016700 94.862 253 13 0 1 253 137 389 3.36e-180 500
MsG0580024219.01.T01 MTR_7g016800 94.466 253 14 0 1 253 137 389 7.98e-180 499
MsG0580024219.01.T01 MTR_7g016820 94.466 253 14 0 1 253 137 389 8.16e-180 499
MsG0580024219.01.T01 MTR_5g007760 94.862 253 13 0 1 253 137 389 8.43e-180 499
MsG0580024219.01.T01 MTR_7g016720 94.466 253 14 0 1 253 137 389 2.78e-179 498
MsG0580024219.01.T01 MTR_7g113405 93.676 253 16 0 1 253 51 303 1.79e-178 493
MsG0580024219.01.T01 MTR_7g016780 93.676 253 16 0 1 253 137 389 3.85e-178 495
MsG0580024219.01.T01 MTR_3g083920 90.873 252 23 0 1 252 137 388 2.33e-174 485
MsG0580024219.01.T01 MTR_3g083910 90.079 252 25 0 1 252 137 388 1.07e-173 484
MsG0580024219.01.T01 MTR_2g058470 86.957 253 33 0 1 253 137 389 6.19e-165 462
MsG0580024219.01.T01 MTR_7g084300 83.399 253 42 0 1 253 137 389 4.87e-159 447
MsG0580024219.01.T01 MTR_5g007740 81.423 253 47 0 1 253 74 326 1.11e-154 433
MsG0580024219.01.T01 MTR_3g086260 80.952 252 48 0 1 252 137 388 5.34e-154 434
MsG0580024219.01.T01 MTR_5g007723 76.680 253 45 1 1 253 160 398 7.12e-139 396
MsG0580024219.01.T01 MTR_3g088675 68.379 253 80 0 1 253 137 389 2.87e-135 387
MsG0580024219.01.T01 MTR_5g007770 75.107 233 25 2 1 233 68 267 2.37e-121 347
MsG0580024219.01.T01 MTR_3g088670 50.769 260 120 4 1 252 129 388 2.20e-89 270
MsG0580024219.01.T01 MTR_4g075560 44.314 255 136 3 1 252 138 389 4.22e-73 228
MsG0580024219.01.T01 MTR_4g078730 39.841 251 145 1 1 251 145 389 6.07e-67 212
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024219.01.T01 AT5G13930 85.039 254 37 1 1 253 142 395 5.92e-159 447
MsG0580024219.01.T01 AT4G34850 41.569 255 146 2 1 253 66 319 9.84e-69 215
MsG0580024219.01.T01 AT4G34850 41.569 255 146 2 1 253 139 392 5.84e-68 215
MsG0580024219.01.T01 AT4G00040 44.094 254 133 4 1 251 138 385 2.39e-67 213
MsG0580024219.01.T01 AT1G02050 42.126 254 138 4 1 251 145 392 1.94e-58 191
MsG0580024219.01.T01 AT3G52160 28.631 241 148 11 14 247 97 320 7.27e-12 64.7
MsG0580024219.01.T01 AT3G52160 28.631 241 148 11 14 247 210 433 1.05e-11 64.7

Find 61 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATAGGTTTCTCAATTTC+TGG 0.117134 5:-2158153 None:intergenic
CTTGTTGGACAAGCACTCTT+TGG 0.251490 5:+2158090 MsG0580024219.01.T01:CDS
GTTTCTCAATTTCTGGTATT+GGG 0.265654 5:-2158146 None:intergenic
GGTTTCTCAATTTCTGGTAT+TGG 0.273222 5:-2158147 None:intergenic
CTGATTACAACTCAATCTTT+TGG 0.293179 5:+2158343 MsG0580024219.01.T01:CDS
AACCTATATTTGAGATGGTT+TGG 0.308691 5:+2158166 MsG0580024219.01.T01:CDS
GCTTCGTTTGGCCAAGGACT+TGG 0.325403 5:+2157975 MsG0580024219.01.T01:CDS
AGAGAAGTACTTAGTGAATA+TGG 0.329263 5:+2158447 MsG0580024219.01.T01:CDS
GATCAAGTTGAACAAAAGTT+AGG 0.336805 5:+2158396 MsG0580024219.01.T01:CDS
CCACAGGAGAAGGGCTTGAT+TGG 0.336949 5:+2158544 MsG0580024219.01.T01:CDS
TTGTAATCAGAAATGTTTAT+TGG 0.344467 5:-2158330 None:intergenic
TGGGGTGTGTTGTTCGGCTT+TGG 0.357346 5:+2158564 MsG0580024219.01.T01:CDS
CACCTTCTTAAAGATGTTCC+TGG 0.385004 5:+2158261 MsG0580024219.01.T01:CDS
CTTGATTGGGGTGTGTTGTT+CGG 0.385641 5:+2158558 MsG0580024219.01.T01:CDS
GTGTTGTTCGGCTTTGGACC+TGG 0.386498 5:+2158570 MsG0580024219.01.T01:CDS
CACAGGAGAAGGGCTTGATT+GGG 0.398609 5:+2158545 MsG0580024219.01.T01:CDS
AGGTGGGACGGTGCTTCGTT+TGG 0.401843 5:+2157963 MsG0580024219.01.T01:CDS
GGAGCTGCTGCACTCATTGT+TGG 0.411529 5:+2158117 MsG0580024219.01.T01:CDS
AATCAGAAATGTTTATTGGT+TGG 0.418046 5:-2158326 None:intergenic
ACTCATTTGGACAGTCTTGT+TGG 0.418448 5:+2158075 MsG0580024219.01.T01:CDS
TCAACTTGATCAAGAATTGC+AGG 0.419690 5:-2158384 None:intergenic
TCAATGGCTCCCTCACTGTC+TGG 0.428576 5:-2158207 None:intergenic
GTCCAAACCATCTCAAATAT+AGG 0.437103 5:-2158168 None:intergenic
GACTTGGCTGAGAACAATAA+AGG 0.438047 5:+2157991 MsG0580024219.01.T01:CDS
TTCACGAAGGTGACCGTCAA+TGG 0.454159 5:-2158223 None:intergenic
GTGCAAAACAACGGTTTCAA+TGG 0.458999 5:-2158598 None:intergenic
ATTGTTCTCAGCCAAGTCCT+TGG 0.461734 5:-2157986 None:intergenic
CACCTGCAAAACATCCTTGT+TGG 0.464560 5:-2157945 None:intergenic
TCAAGAATTGCAGGTCCACC+TGG 0.467633 5:-2158375 None:intergenic
CGAAAGAAATCTGCTCAAGA+AGG 0.474015 5:+2158513 MsG0580024219.01.T01:CDS
ACCTTCTTAAAGATGTTCCT+GGG 0.484500 5:+2158262 MsG0580024219.01.T01:CDS
GACAGTGAGGGAGCCATTGA+CGG 0.489536 5:+2158210 MsG0580024219.01.T01:CDS
TGGTCCTAGTGATACTCATT+TGG 0.493191 5:+2158062 MsG0580024219.01.T01:CDS
GACGGTGCTTCGTTTGGCCA+AGG 0.496087 5:+2157969 MsG0580024219.01.T01:CDS
GTCTTCGTCCATATGTGAAA+AGG 0.504788 5:+2157911 MsG0580024219.01.T01:CDS
GGGCTTAAGACCACAGGAGA+AGG 0.504979 5:+2158534 MsG0580024219.01.T01:CDS
TGAGAAACCTATATTTGAGA+TGG 0.507221 5:+2158161 MsG0580024219.01.T01:CDS
GTGAGTTGGTAATCGGCACC+AGG 0.519680 5:-2157877 None:intergenic
CATTGATAAAGCATTGATTG+AGG 0.521723 5:+2158299 MsG0580024219.01.T01:CDS
GACGGTCACCTTCGTGAAGT+TGG 0.532576 5:+2158228 MsG0580024219.01.T01:CDS
GGACAAGCACTCTTTGGAGA+TGG 0.536691 5:+2158096 MsG0580024219.01.T01:CDS
ATGTTCTTTGAAACAATCCC+AGG 0.542390 5:-2158279 None:intergenic
TCCCAGGAACATCTTTAAGA+AGG 0.550142 5:-2158263 None:intergenic
CCAATCAAGCCCTTCTCCTG+TGG 0.556018 5:-2158544 None:intergenic
CTGTCCAAATGAGTATCACT+AGG 0.561644 5:-2158066 None:intergenic
TTCACTAAGTACTTCTCTAG+TGG 0.572078 5:-2158442 None:intergenic
ACGGTTTCAATGGTAAGTCC+AGG 0.578176 5:-2158588 None:intergenic
GAAAGAAATCTGCTCAAGAA+GGG 0.590492 5:+2158514 MsG0580024219.01.T01:CDS
ACAAGTGGTGTGGACATGCC+TGG 0.595968 5:+2157859 None:intergenic
CAAGAAGGGCTTAAGACCAC+AGG 0.597698 5:+2158528 MsG0580024219.01.T01:CDS
CAATAAAGGTGCTCGTGTGT+TGG 0.608320 5:+2158005 MsG0580024219.01.T01:CDS
GGCTTAAGACCACAGGAGAA+GGG 0.617893 5:+2158535 MsG0580024219.01.T01:CDS
ACAGGAGAAGGGCTTGATTG+GGG 0.623900 5:+2158546 MsG0580024219.01.T01:CDS
AATGAGTATCACTAGGACCA+CGG 0.635157 5:-2158059 None:intergenic
AACAATTGCTCCAGACAGTG+AGG 0.638797 5:+2158197 MsG0580024219.01.T01:CDS
CAAGAATTGCAGGTCCACCT+GGG 0.641015 5:-2158374 None:intergenic
GTTACTGCTGTGACATTCCG+TGG 0.652143 5:+2158042 MsG0580024219.01.T01:CDS
ACAATTGCTCCAGACAGTGA+GGG 0.658063 5:+2158198 MsG0580024219.01.T01:CDS
AGCCACACTGTGCAAAACAA+CGG 0.665293 5:-2158607 None:intergenic
AGGTATATGATGTACCAACA+AGG 0.699849 5:+2157931 MsG0580024219.01.T01:CDS
ATGTTAGACCAACTTCACGA+AGG 0.749180 5:-2158236 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTAATCAGAAATGTTTAT+TGG - Chr5:2158333-2158352 None:intergenic 20.0%
! AATATAGGTTTCTCAATTTC+TGG - Chr5:2158156-2158175 None:intergenic 25.0%
! AATCAGAAATGTTTATTGGT+TGG - Chr5:2158329-2158348 None:intergenic 25.0%
!! ATCATATACCTTTTCACATA+TGG - Chr5:2157922-2157941 None:intergenic 25.0%
TGAGAAACCTATATTTGAGA+TGG + Chr5:2158161-2158180 MsG0580024219.01.T01:CDS 30.0%
! AACCTATATTTGAGATGGTT+TGG + Chr5:2158166-2158185 MsG0580024219.01.T01:CDS 30.0%
! CTGATTACAACTCAATCTTT+TGG + Chr5:2158343-2158362 MsG0580024219.01.T01:CDS 30.0%
! TACCAACTCACAAAACTTTT+AGG + Chr5:2157889-2157908 MsG0580024219.01.T01:CDS 30.0%
!! AGAGAAGTACTTAGTGAATA+TGG + Chr5:2158447-2158466 MsG0580024219.01.T01:CDS 30.0%
!! CATTGATAAAGCATTGATTG+AGG + Chr5:2158299-2158318 MsG0580024219.01.T01:CDS 30.0%
!! GATCAAGTTGAACAAAAGTT+AGG + Chr5:2158396-2158415 MsG0580024219.01.T01:CDS 30.0%
!! GTTTCTCAATTTCTGGTATT+GGG - Chr5:2158149-2158168 None:intergenic 30.0%
!!! AAGTTTTGTGAGTTGGTAAT+CGG - Chr5:2157887-2157906 None:intergenic 30.0%
ACCTTCTTAAAGATGTTCCT+GGG + Chr5:2158262-2158281 MsG0580024219.01.T01:CDS 35.0%
AGGTATATGATGTACCAACA+AGG + Chr5:2157931-2157950 MsG0580024219.01.T01:CDS 35.0%
GAAAGAAATCTGCTCAAGAA+GGG + Chr5:2158514-2158533 MsG0580024219.01.T01:CDS 35.0%
GTCCAAACCATCTCAAATAT+AGG - Chr5:2158171-2158190 None:intergenic 35.0%
TCAACTTGATCAAGAATTGC+AGG - Chr5:2158387-2158406 None:intergenic 35.0%
TTCACTAAGTACTTCTCTAG+TGG - Chr5:2158445-2158464 None:intergenic 35.0%
!! ATGTTCTTTGAAACAATCCC+AGG - Chr5:2158282-2158301 None:intergenic 35.0%
!! GACCTAAAAGTTTTGTGAGT+TGG - Chr5:2157894-2157913 None:intergenic 35.0%
!! GGTTTCTCAATTTCTGGTAT+TGG - Chr5:2158150-2158169 None:intergenic 35.0%
AATGAGTATCACTAGGACCA+CGG - Chr5:2158062-2158081 None:intergenic 40.0%
ATGTTAGACCAACTTCACGA+AGG - Chr5:2158239-2158258 None:intergenic 40.0%
CACCTTCTTAAAGATGTTCC+TGG + Chr5:2158261-2158280 MsG0580024219.01.T01:CDS 40.0%
CGAAAGAAATCTGCTCAAGA+AGG + Chr5:2158513-2158532 MsG0580024219.01.T01:CDS 40.0%
CTGTCCAAATGAGTATCACT+AGG - Chr5:2158069-2158088 None:intergenic 40.0%
GACTTGGCTGAGAACAATAA+AGG + Chr5:2157991-2158010 MsG0580024219.01.T01:CDS 40.0%
GTCTTCGTCCATATGTGAAA+AGG + Chr5:2157911-2157930 MsG0580024219.01.T01:CDS 40.0%
GTGCAAAACAACGGTTTCAA+TGG - Chr5:2158601-2158620 None:intergenic 40.0%
TGGTCCTAGTGATACTCATT+TGG + Chr5:2158062-2158081 MsG0580024219.01.T01:CDS 40.0%
! AACAAGGATGTTTTGCAGGT+GGG + Chr5:2157947-2157966 MsG0580024219.01.T01:CDS 40.0%
! ACTCATTTGGACAGTCTTGT+TGG + Chr5:2158075-2158094 MsG0580024219.01.T01:CDS 40.0%
! CTAGTGGCTTTCATCTTTTC+AGG - Chr5:2158429-2158448 None:intergenic 40.0%
! TACCAACAAGGATGTTTTGC+AGG + Chr5:2157943-2157962 MsG0580024219.01.T01:CDS 40.0%
! TCCCAGGAACATCTTTAAGA+AGG - Chr5:2158266-2158285 None:intergenic 40.0%
AACAATTGCTCCAGACAGTG+AGG + Chr5:2158197-2158216 MsG0580024219.01.T01:CDS 45.0%
ACAATTGCTCCAGACAGTGA+GGG + Chr5:2158198-2158217 MsG0580024219.01.T01:CDS 45.0%
ACGGTTTCAATGGTAAGTCC+AGG - Chr5:2158591-2158610 None:intergenic 45.0%
AGCCACACTGTGCAAAACAA+CGG - Chr5:2158610-2158629 None:intergenic 45.0%
ATTGTTCTCAGCCAAGTCCT+TGG - Chr5:2157989-2158008 None:intergenic 45.0%
CACCTGCAAAACATCCTTGT+TGG - Chr5:2157948-2157967 None:intergenic 45.0%
CTTGTTGGACAAGCACTCTT+TGG + Chr5:2158090-2158109 MsG0580024219.01.T01:CDS 45.0%
! ATCTTTTGGATTGCTCACCC+AGG + Chr5:2158357-2158376 MsG0580024219.01.T01:CDS 45.0%
! CAACAAGGATGTTTTGCAGG+TGG + Chr5:2157946-2157965 MsG0580024219.01.T01:CDS 45.0%
! CTTGATTGGGGTGTGTTGTT+CGG + Chr5:2158558-2158577 MsG0580024219.01.T01:CDS 45.0%
!! AACCGTTGTTTTGCACAGTG+TGG + Chr5:2158605-2158624 MsG0580024219.01.T01:CDS 45.0%
!! CAATAAAGGTGCTCGTGTGT+TGG + Chr5:2158005-2158024 MsG0580024219.01.T01:CDS 45.0%
ACAGGAGAAGGGCTTGATTG+GGG + Chr5:2158546-2158565 MsG0580024219.01.T01:CDS 50.0%
CAAGAAGGGCTTAAGACCAC+AGG + Chr5:2158528-2158547 MsG0580024219.01.T01:CDS 50.0%
CAAGAATTGCAGGTCCACCT+GGG - Chr5:2158377-2158396 None:intergenic 50.0%
CACAGGAGAAGGGCTTGATT+GGG + Chr5:2158545-2158564 MsG0580024219.01.T01:CDS 50.0%
GGCTTAAGACCACAGGAGAA+GGG + Chr5:2158535-2158554 MsG0580024219.01.T01:CDS 50.0%
GTTACTGCTGTGACATTCCG+TGG + Chr5:2158042-2158061 MsG0580024219.01.T01:CDS 50.0%
TCAAGAATTGCAGGTCCACC+TGG - Chr5:2158378-2158397 None:intergenic 50.0%
TTCACGAAGGTGACCGTCAA+TGG - Chr5:2158226-2158245 None:intergenic 50.0%
! AGGATGTTTTGCAGGTGGGA+CGG + Chr5:2157951-2157970 MsG0580024219.01.T01:CDS 50.0%
! GGACAAGCACTCTTTGGAGA+TGG + Chr5:2158096-2158115 MsG0580024219.01.T01:CDS 50.0%
! TTTTGGATTGCTCACCCAGG+TGG + Chr5:2158360-2158379 MsG0580024219.01.T01:CDS 50.0%
CCAATCAAGCCCTTCTCCTG+TGG - Chr5:2158547-2158566 None:intergenic 55.0%
CCACAGGAGAAGGGCTTGAT+TGG + Chr5:2158544-2158563 MsG0580024219.01.T01:CDS 55.0%
GACAGTGAGGGAGCCATTGA+CGG + Chr5:2158210-2158229 MsG0580024219.01.T01:CDS 55.0%
GACGGTCACCTTCGTGAAGT+TGG + Chr5:2158228-2158247 MsG0580024219.01.T01:CDS 55.0%
GCTTCGTTTGGCCAAGGACT+TGG + Chr5:2157975-2157994 MsG0580024219.01.T01:CDS 55.0%
GGAGCTGCTGCACTCATTGT+TGG + Chr5:2158117-2158136 MsG0580024219.01.T01:CDS 55.0%
GGGCTTAAGACCACAGGAGA+AGG + Chr5:2158534-2158553 MsG0580024219.01.T01:CDS 55.0%
TCAATGGCTCCCTCACTGTC+TGG - Chr5:2158210-2158229 None:intergenic 55.0%
! TGGGGTGTGTTGTTCGGCTT+TGG + Chr5:2158564-2158583 MsG0580024219.01.T01:CDS 55.0%
!! GTGAGTTGGTAATCGGCACC+AGG - Chr5:2157880-2157899 None:intergenic 55.0%
!! GTGTTGTTCGGCTTTGGACC+TGG + Chr5:2158570-2158589 MsG0580024219.01.T01:CDS 55.0%
AGGTGGGACGGTGCTTCGTT+TGG + Chr5:2157963-2157982 MsG0580024219.01.T01:CDS 60.0%
GACGGTGCTTCGTTTGGCCA+AGG + Chr5:2157969-2157988 MsG0580024219.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 2157874 2158635 2157874 ID=MsG0580024219.01;Name=MsG0580024219.01
Chr5 mRNA 2157874 2158635 2157874 ID=MsG0580024219.01.T01;Parent=MsG0580024219.01;Name=MsG0580024219.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|253
Chr5 exon 2157874 2158635 2157874 ID=MsG0580024219.01.T01:exon:624;Parent=MsG0580024219.01.T01
Chr5 CDS 2157874 2158635 2157874 ID=MsG0580024219.01.T01:cds;Parent=MsG0580024219.01.T01
Gene Sequence

>MsG0580024219.01.T01

ATGCCTGGTGCCGATTACCAACTCACAAAACTTTTAGGTCTTCGTCCATATGTGAAAAGGTATATGATGTACCAACAAGGATGTTTTGCAGGTGGGACGGTGCTTCGTTTGGCCAAGGACTTGGCTGAGAACAATAAAGGTGCTCGTGTGTTGGTTGTTTGTTCTGAAGTTACTGCTGTGACATTCCGTGGTCCTAGTGATACTCATTTGGACAGTCTTGTTGGACAAGCACTCTTTGGAGATGGAGCTGCTGCACTCATTGTTGGTTCTGACCCAATACCAGAAATTGAGAAACCTATATTTGAGATGGTTTGGACTGCACAAACAATTGCTCCAGACAGTGAGGGAGCCATTGACGGTCACCTTCGTGAAGTTGGTCTAACATTTCACCTTCTTAAAGATGTTCCTGGGATTGTTTCAAAGAACATTGATAAAGCATTGATTGAGGCTTTCCAACCAATAAACATTTCTGATTACAACTCAATCTTTTGGATTGCTCACCCAGGTGGACCTGCAATTCTTGATCAAGTTGAACAAAAGTTAGGCTTAAAACCTGAAAAGATGAAAGCCACTAGAGAAGTACTTAGTGAATATGGTAACATGTCAAGTGCATGTGTATTGTTTATCTTAGATGAGATGCGAAAGAAATCTGCTCAAGAAGGGCTTAAGACCACAGGAGAAGGGCTTGATTGGGGTGTGTTGTTCGGCTTTGGACCTGGACTTACCATTGAAACCGTTGTTTTGCACAGTGTGGCTATATGA

Protein sequence

>MsG0580024219.01.T01

MPGADYQLTKLLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSEVTAVTFRGPSDTHLDSLVGQALFGDGAAALIVGSDPIPEIEKPIFEMVWTAQTIAPDSEGAIDGHLREVGLTFHLLKDVPGIVSKNIDKALIEAFQPINISDYNSIFWIAHPGGPAILDQVEQKLGLKPEKMKATREVLSEYGNMSSACVLFILDEMRKKSAQEGLKTTGEGLDWGVLFGFGPGLTIETVVLHSVAI*