AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025412.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025412.01.T01 MTR_5g028010 95.763 118 5 0 14 131 51 168 8.77e-80 232
MsG0580025412.01.T01 MTR_5g020120 66.667 108 36 0 19 126 21 128 1.73e-51 159
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025412.01.T01 AT1G68310 84.112 107 17 0 20 126 49 155 1.66e-64 193
MsG0580025412.01.T01 AT1G68310 84.112 107 17 0 20 126 47 153 3.67e-64 192
MsG0580025412.01.T01 AT3G50845 67.290 107 35 0 20 126 43 149 3.19e-50 157
MsG0580025412.01.T01 AT3G09380 68.519 108 33 1 20 126 39 146 4.56e-48 151

Find 42 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTATAGGTCTTTGTTTA+CGG 0.252751 5:-18223645 MsG0580025412.01.T01:CDS
TTGTAGTTCAATATGATTAT+AGG 0.279752 5:-18220667 MsG0580025412.01.T01:three_prime_UTR
AAATCCAAACCTTGTGGATA+TGG 0.295982 5:-18220867 MsG0580025412.01.T01:CDS
TCCTGAGCATCCTTACTCTT+TGG 0.296343 5:-18223869 MsG0580025412.01.T01:CDS
TTACTTAGGTTTCATAGTTA+TGG 0.334471 5:-18220561 MsG0580025412.01.T01:three_prime_UTR
ATTAGCTCTAGAGAAGTAAA+TGG 0.354771 5:-18220593 MsG0580025412.01.T01:three_prime_UTR
TCAACCATATCCACAAGGTT+TGG 0.362140 5:+18220863 None:intergenic
AATTATAGGTCTTTGTTTAC+GGG 0.379203 5:-18223644 MsG0580025412.01.T01:CDS
ATTTCTAAAACAAAACATAA+AGG 0.382817 5:+18224011 None:intergenic
TGCAGTATGGCAACAATTAT+AGG 0.384556 5:-18223658 MsG0580025412.01.T01:CDS
CCCACAAGACTCGTAGAATT+AGG 0.396254 5:+18220797 None:intergenic
TATTTAAATAGAGTAATCAT+AGG 0.418534 5:+18220495 None:intergenic
TTGAGTTCTTCCAAAGAGTA+AGG 0.436279 5:+18223859 None:intergenic
GCTTCAGTTGCATGAGATCC+AGG 0.451024 5:+18221362 None:intergenic
TTTCTAAAACAAAACATAAA+GGG 0.461076 5:+18224012 None:intergenic
GACAGTGGAACATTGCAGTA+TGG 0.471232 5:-18223671 MsG0580025412.01.T01:CDS
CCTAATTCTACGAGTCTTGT+GGG 0.472596 5:-18220797 MsG0580025412.01.T01:three_prime_UTR
GAACTACAATTACATGTAGT+AGG 0.475009 5:+18220681 None:intergenic
TCCTAATTCTACGAGTCTTG+TGG 0.475328 5:-18220798 MsG0580025412.01.T01:three_prime_UTR
TCCAAAGAGTAAGGATGCTC+AGG 0.478267 5:+18223868 None:intergenic
AAATAAAGTTGAACGACTAC+TGG 0.486648 5:+18220757 None:intergenic
AAATCATATAAGAGATATCA+AGG 0.501737 5:-18223893 MsG0580025412.01.T01:intron
ATGATCAGAAAAGTTATGTC+AGG 0.508593 5:-18223807 MsG0580025412.01.T01:intron
GTTGAACGACTACTGGCATA+AGG 0.518529 5:+18220764 None:intergenic
GGTTACGTTTACTCCGACAG+TGG 0.527475 5:-18223686 MsG0580025412.01.T01:CDS
AAGTTTGCCTCCGCGGTTCA+AGG 0.531640 5:-18223608 MsG0580025412.01.T01:intron
TTACTCTTTGGAAGAACTCA+AGG 0.536558 5:-18223857 MsG0580025412.01.T01:CDS
GTGATAGTTGAGTAGTATCA+AGG 0.546344 5:-18220626 MsG0580025412.01.T01:three_prime_UTR
TCTCATGCAACTGAAGCTGC+AGG 0.547140 5:-18221356 MsG0580025412.01.T01:intron
AAGTTCCTTCAATCAAAAGA+TGG 0.559241 5:+18220827 None:intergenic
GTGGACATCAGAGTGGCACC+TGG 0.564431 5:-18221380 MsG0580025412.01.T01:CDS
TCTTGAGAAGTTTGCCTCCG+CGG 0.580476 5:-18223615 MsG0580025412.01.T01:CDS
ATAAAATTTACCTTGAACCG+CGG 0.583797 5:+18223598 None:intergenic
TTCAATCAAAAGATGGCGCA+AGG 0.585537 5:+18220834 None:intergenic
ATAGAGTAATCATAGGAATG+TGG 0.623735 5:+18220502 None:intergenic
ATACTGCAATGTTCCACTGT+CGG 0.625856 5:+18223673 None:intergenic
ACAAGACTCGTAGAATTAGG+AGG 0.644675 5:+18220800 None:intergenic
TGGTTAGGTGGACATCAGAG+TGG 0.646704 5:-18221387 MsG0580025412.01.T01:intron
ACTTGAAAATCCAAACCTTG+TGG 0.649054 5:-18220873 MsG0580025412.01.T01:CDS
AAATTTACCTTGAACCGCGG+AGG 0.651737 5:+18223601 None:intergenic
AGAACTCAAGGTGATTACTG+AGG 0.702667 5:-18223845 MsG0580025412.01.T01:CDS
ATTCATCAACCATATCCACA+AGG 0.710416 5:+18220858 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTCATATTATTGATTATT+CGG + Chr5:18221246-18221265 None:intergenic 10.0%
!!! TATTGGATTATTTTTATTTA+GGG - Chr5:18220789-18220808 MsG0580025412.01.T01:three_prime_UTR 10.0%
!!! TATTTTGTGTTTTAAAAAAA+TGG + Chr5:18222998-18223017 None:intergenic 10.0%
!!! TTATTGGATTATTTTTATTT+AGG - Chr5:18220788-18220807 MsG0580025412.01.T01:three_prime_UTR 10.0%
!! AATAAGTTAAAGAAGAAATT+TGG - Chr5:18222195-18222214 MsG0580025412.01.T01:intron 15.0%
!! ATATGATTTAAGAATTGTTA+AGG + Chr5:18220592-18220611 None:intergenic 15.0%
!! GAATAATCAATAATATGAAA+TGG - Chr5:18221245-18221264 MsG0580025412.01.T01:intron 15.0%
!! TATTTAAATAGAGTAATCAT+AGG + Chr5:18224004-18224023 None:intergenic 15.0%
!! TTATAACATAACATAAAATG+TGG - Chr5:18221128-18221147 MsG0580025412.01.T01:intron 15.0%
!!! ATGATTTTAAAATTTTGGTT+AGG - Chr5:18223094-18223113 MsG0580025412.01.T01:intron 15.0%
!!! ATTTCTAAAACAAAACATAA+AGG + Chr5:18220488-18220507 None:intergenic 15.0%
!!! TATTTTTTCTTTCAAGTATT+TGG - Chr5:18220723-18220742 MsG0580025412.01.T01:three_prime_UTR 15.0%
!!! TCTTGATGATTTTAAAATTT+TGG - Chr5:18223089-18223108 MsG0580025412.01.T01:intron 15.0%
!!! TTTATTATTTCTGTTTTAAG+AGG + Chr5:18221383-18221402 None:intergenic 15.0%
!!! TTTCTAAAACAAAACATAAA+GGG + Chr5:18220487-18220506 None:intergenic 15.0%
!!! TTTGTATGATATTTTTGTTT+TGG - Chr5:18223759-18223778 MsG0580025412.01.T01:intron 15.0%
!! AAATCATATAAGAGATATCA+AGG - Chr5:18220603-18220622 MsG0580025412.01.T01:three_prime_UTR 20.0%
!! AATCAATAATATGAAATGGA+TGG - Chr5:18221249-18221268 MsG0580025412.01.T01:intron 20.0%
!! ATCAATAATATGAAATGGAT+GGG - Chr5:18221250-18221269 MsG0580025412.01.T01:intron 20.0%
!! ATTTAATAGGATATTGAAAG+TGG - Chr5:18222687-18222706 MsG0580025412.01.T01:intron 20.0%
!! TTGTAGTTCAATATGATTAT+AGG - Chr5:18223829-18223848 MsG0580025412.01.T01:CDS 20.0%
!! TTTGATTATCAGATAAAGAT+AGG - Chr5:18221439-18221458 MsG0580025412.01.T01:intron 20.0%
!! TTTGTAAAAGAGTTTACAAA+GGG + Chr5:18222332-18222351 None:intergenic 20.0%
!!! AATGGAAATTCTTTTTACTT+AGG - Chr5:18223921-18223940 MsG0580025412.01.T01:intron 20.0%
!!! ATTTTAAAATTTTGGTTAGG+TGG - Chr5:18223097-18223116 MsG0580025412.01.T01:intron 20.0%
!!! CCTTAGATTTTGATTATTTT+GGG - Chr5:18223371-18223390 MsG0580025412.01.T01:intron 20.0%
!!! CTTAGATTTTGATTATTTTG+GGG - Chr5:18223372-18223391 MsG0580025412.01.T01:intron 20.0%
!!! CTTTTCCTTCTTAATATTTT+GGG + Chr5:18221530-18221549 None:intergenic 20.0%
!!! GATTAATGTGTTTGTTTTAT+TGG - Chr5:18220772-18220791 MsG0580025412.01.T01:three_prime_UTR 20.0%
!!! GATTTGATACTTTGTTTTAT+TGG - Chr5:18223411-18223430 MsG0580025412.01.T01:intron 20.0%
!!! TCTTTTCCTTCTTAATATTT+TGG + Chr5:18221531-18221550 None:intergenic 20.0%
!!! TTTTGTAAAAGAGTTTACAA+AGG + Chr5:18222333-18222352 None:intergenic 20.0%
! AACATAACATAAAATGTGGT+AGG - Chr5:18221132-18221151 MsG0580025412.01.T01:intron 25.0%
! ACATAACATAAAATGTGGTA+GGG - Chr5:18221133-18221152 MsG0580025412.01.T01:intron 25.0%
! AGTAGATAGAGTTGTTATAT+AGG - Chr5:18222744-18222763 MsG0580025412.01.T01:intron 25.0%
! ATTGTAACTGTAATATTGCA+CGG - Chr5:18221168-18221187 MsG0580025412.01.T01:intron 25.0%
! CCCAAAATAATCAAAATCTA+AGG + Chr5:18223374-18223393 None:intergenic 25.0%
! CTTTATCTGATAATCAAAGA+CGG + Chr5:18221437-18221456 None:intergenic 25.0%
! GAGGGATTGAAATATTTAAT+AGG - Chr5:18222674-18222693 MsG0580025412.01.T01:intron 25.0%
! GTAGATAGAGTTGTTATATA+GGG - Chr5:18222745-18222764 MsG0580025412.01.T01:intron 25.0%
! GTATCATTATCTGCTATAAA+GGG - Chr5:18221726-18221745 MsG0580025412.01.T01:intron 25.0%
! GTTATAAATCATCATTGACA+AGG + Chr5:18221115-18221134 None:intergenic 25.0%
! TTACTTAGGTTTCATAGTTA+TGG - Chr5:18223935-18223954 MsG0580025412.01.T01:intron 25.0%
!! AATTATAGGTCTTTGTTTAC+GGG - Chr5:18220852-18220871 MsG0580025412.01.T01:CDS 25.0%
!! ATATTATTGATTATTCGGTG+AGG + Chr5:18221241-18221260 None:intergenic 25.0%
!! CAATTATAGGTCTTTGTTTA+CGG - Chr5:18220851-18220870 MsG0580025412.01.T01:CDS 25.0%
!! CATTTGTGTTACATGTATTT+TGG - Chr5:18221961-18221980 MsG0580025412.01.T01:intron 25.0%
!! TAACACAAATGTTTTTCGTT+GGG + Chr5:18221952-18221971 None:intergenic 25.0%
!! TAAGGCAATTTTTCTGTAAA+AGG + Chr5:18223356-18223375 None:intergenic 25.0%
!! TCAAGTATTTGGTTTATACT+TGG - Chr5:18220734-18220753 MsG0580025412.01.T01:three_prime_UTR 25.0%
!!! CACTTTTAATTCTATTTTGC+AGG + Chr5:18221988-18222007 None:intergenic 25.0%
!!! GCCTTAGATTTTGATTATTT+TGG - Chr5:18223370-18223389 MsG0580025412.01.T01:intron 25.0%
!!! TTGCTTTTATTATGTAGGAT+AGG - Chr5:18222466-18222485 MsG0580025412.01.T01:intron 25.0%
AAAAAAGAAATGAAGAGGCA+TGG - Chr5:18222711-18222730 MsG0580025412.01.T01:intron 30.0%
AAATAAAGTTGAACGACTAC+TGG + Chr5:18223742-18223761 None:intergenic 30.0%
AAATACAGAGAGTTCATTTG+AGG + Chr5:18222594-18222613 None:intergenic 30.0%
AAGTTCCTTCAATCAAAAGA+TGG + Chr5:18223672-18223691 None:intergenic 30.0%
AGGTGAATTCTAATATCATC+CGG + Chr5:18222302-18222321 None:intergenic 30.0%
ATAAAATTTACCTTGAACCG+CGG + Chr5:18220901-18220920 None:intergenic 30.0%
ATAGAGTAATCATAGGAATG+TGG + Chr5:18223997-18224016 None:intergenic 30.0%
ATGATCAGAAAAGTTATGTC+AGG - Chr5:18220689-18220708 MsG0580025412.01.T01:three_prime_UTR 30.0%
CACATTGACATTATCCTTAA+CGG - Chr5:18222163-18222182 MsG0580025412.01.T01:intron 30.0%
CCTATCCCAAAATATTAAGA+AGG - Chr5:18221522-18221541 MsG0580025412.01.T01:intron 30.0%
CTGTTTGCATAATGTAAGAT+GGG - Chr5:18222652-18222671 MsG0580025412.01.T01:intron 30.0%
GAACTACAATTACATGTAGT+AGG + Chr5:18223818-18223837 None:intergenic 30.0%
GAGTTGTTATATAGGGAAAA+TGG - Chr5:18222752-18222771 MsG0580025412.01.T01:intron 30.0%
GATAATGATACCTCATTCTT+TGG + Chr5:18221651-18221670 None:intergenic 30.0%
GGTATCATTATCTGCTATAA+AGG - Chr5:18221725-18221744 MsG0580025412.01.T01:intron 30.0%
TAAAAGAGTTTACAAAGGGT+GGG + Chr5:18222328-18222347 None:intergenic 30.0%
TAAGAATAGGAAAGATCAAG+TGG - Chr5:18222834-18222853 MsG0580025412.01.T01:intron 30.0%
TACATCACTAAACAAAACTC+AGG + Chr5:18220940-18220959 None:intergenic 30.0%
TCATGCAATCTGTAATTACT+TGG + Chr5:18223025-18223044 None:intergenic 30.0%
TCTAGATCATAAGTCATAAC+TGG - Chr5:18223494-18223513 MsG0580025412.01.T01:intron 30.0%
TTTAGGCAGACAAACTTATT+AGG - Chr5:18222911-18222930 MsG0580025412.01.T01:intron 30.0%
! AAGACGGATATTCTGTTTTA+AGG + Chr5:18221421-18221440 None:intergenic 30.0%
! AAGCTAAATGAACACGATTT+TGG - Chr5:18222556-18222575 MsG0580025412.01.T01:intron 30.0%
! AGATAGACTATTTTGTTGCT+TGG - Chr5:18220975-18220994 MsG0580025412.01.T01:intron 30.0%
! GTAACACAAATGTTTTTCGT+TGG + Chr5:18221953-18221972 None:intergenic 30.0%
! GTTCTTGCTTGATATTTTCT+GGG - Chr5:18221214-18221233 MsG0580025412.01.T01:intron 30.0%
! TAAAGTACTACTGAATGCTT+AGG + Chr5:18223249-18223268 None:intergenic 30.0%
! TATTTGGTTTATACTTGGTG+TGG - Chr5:18220739-18220758 MsG0580025412.01.T01:three_prime_UTR 30.0%
! TGTTCTTGCTTGATATTTTC+TGG - Chr5:18221213-18221232 MsG0580025412.01.T01:intron 30.0%
! TGTTGCTTGGTAACATATAA+TGG - Chr5:18220988-18221007 MsG0580025412.01.T01:intron 30.0%
!! AAAAGTGTTAGCAGTTGAAA+AGG - Chr5:18222001-18222020 MsG0580025412.01.T01:intron 30.0%
!! ACAAAATCCTCTAAAACACA+TGG + Chr5:18223220-18223239 None:intergenic 30.0%
!! AGACGGATATTCTGTTTTAA+GGG + Chr5:18221420-18221439 None:intergenic 30.0%
!! AGGAAAAGAGCTCTAAAATT+TGG - Chr5:18221542-18221561 MsG0580025412.01.T01:intron 30.0%
!! ATTAGCTCTAGAGAAGTAAA+TGG - Chr5:18223903-18223922 MsG0580025412.01.T01:intron 30.0%
!! CAAAATCCTCTAAAACACAT+GGG + Chr5:18223219-18223238 None:intergenic 30.0%
!! TTTATACTTGGTGTGGATTA+TGG - Chr5:18220746-18220765 MsG0580025412.01.T01:three_prime_UTR 30.0%
!!! AAATGAACACGATTTTGGTT+TGG - Chr5:18222561-18222580 MsG0580025412.01.T01:intron 30.0%
!!! ATTTTTGTTTTGGATTGCTG+AGG - Chr5:18223769-18223788 MsG0580025412.01.T01:intron 30.0%
!!! CCTTCTTAATATTTTGGGAT+AGG + Chr5:18221525-18221544 None:intergenic 30.0%
!!! TTAATATTTTGGGATAGGCT+TGG + Chr5:18221520-18221539 None:intergenic 30.0%
AAAAGAGTTTACAAAGGGTG+GGG + Chr5:18222327-18222346 None:intergenic 35.0%
AAACGAAGGGGAATAAGAAT+AGG - Chr5:18222821-18222840 MsG0580025412.01.T01:intron 35.0%
AAAGATCAAGTGGTCATACA+TGG - Chr5:18222844-18222863 MsG0580025412.01.T01:intron 35.0%
AAATCCAAACCTTGTGGATA+TGG - Chr5:18223629-18223648 MsG0580025412.01.T01:CDS 35.0%
AAGATCAAGTGGTCATACAT+GGG - Chr5:18222845-18222864 MsG0580025412.01.T01:intron 35.0%
ACAAACATAATCCAGCATAG+GGG + Chr5:18223070-18223089 None:intergenic 35.0%
ACTTATGATCTAGAAACGTG+TGG + Chr5:18223488-18223507 None:intergenic 35.0%
ACTTGAAAATCCAAACCTTG+TGG - Chr5:18223623-18223642 MsG0580025412.01.T01:CDS 35.0%
AGACAAACATAATCCAGCAT+AGG + Chr5:18223072-18223091 None:intergenic 35.0%
ATCAGCAATTCCAAAGAATG+AGG - Chr5:18221704-18221723 MsG0580025412.01.T01:intron 35.0%
ATGTCAATGTGTCTTGTGAA+TGG + Chr5:18222154-18222173 None:intergenic 35.0%
ATGTCATCACTTTAACCCAT+AGG - Chr5:18222415-18222434 MsG0580025412.01.T01:intron 35.0%
ATTCATCAACCATATCCACA+AGG + Chr5:18223641-18223660 None:intergenic 35.0%
CAACAGAAAACAAGACCTAT+GGG + Chr5:18222433-18222452 None:intergenic 35.0%
GAAACACAGTTTCATTCTTC+TGG - Chr5:18221909-18221928 MsG0580025412.01.T01:intron 35.0%
GACAAACATAATCCAGCATA+GGG + Chr5:18223071-18223090 None:intergenic 35.0%
GCAGTATACACACAAGTATA+GGG + Chr5:18223195-18223214 None:intergenic 35.0%
GCTGTTTGCATAATGTAAGA+TGG - Chr5:18222651-18222670 MsG0580025412.01.T01:intron 35.0%
GTAAAAGAGTTTACAAAGGG+TGG + Chr5:18222329-18222348 None:intergenic 35.0%
GTGATAGTTGAGTAGTATCA+AGG - Chr5:18223870-18223889 MsG0580025412.01.T01:CDS 35.0%
GTGGCAAAAAAGAAATGAAG+AGG - Chr5:18222706-18222725 MsG0580025412.01.T01:intron 35.0%
TGCAGTATACACACAAGTAT+AGG + Chr5:18223196-18223215 None:intergenic 35.0%
TGCAGTATGGCAACAATTAT+AGG - Chr5:18220838-18220857 MsG0580025412.01.T01:CDS 35.0%
TGGAGACAATGATAGATTGA+CGG + Chr5:18222978-18222997 None:intergenic 35.0%
TGTCAATGTGTCTTGTGAAT+GGG + Chr5:18222153-18222172 None:intergenic 35.0%
TTAAATTCTTGAGTGACCCT+TGG + Chr5:18222257-18222276 None:intergenic 35.0%
TTATTCATATGCAGCCGTTA+AGG + Chr5:18222180-18222199 None:intergenic 35.0%
TTGAGTTCTTCCAAAGAGTA+AGG + Chr5:18220640-18220659 None:intergenic 35.0%
TTGCATAATGTAAGATGGGA+GGG - Chr5:18222656-18222675 MsG0580025412.01.T01:intron 35.0%
TTTGCATAATGTAAGATGGG+AGG - Chr5:18222655-18222674 MsG0580025412.01.T01:intron 35.0%
! AAACTGTTTTGCATTGCTTC+AGG + Chr5:18221782-18221801 None:intergenic 35.0%
! AACTGTTTTGCATTGCTTCA+GGG + Chr5:18221781-18221800 None:intergenic 35.0%
! AGAATGAAACTGTGTTTCTG+TGG + Chr5:18221907-18221926 None:intergenic 35.0%
! AGCTTTCTTAGGAGTACAAA+TGG - Chr5:18222524-18222543 MsG0580025412.01.T01:intron 35.0%
! ATCGTGTTCATTTAGCTTCA+TGG + Chr5:18222554-18222573 None:intergenic 35.0%
! GCTTTCTTAGGAGTACAAAT+GGG - Chr5:18222525-18222544 MsG0580025412.01.T01:intron 35.0%
! GCTTTTCCACTTGGATTTTA+GGG - Chr5:18222364-18222383 MsG0580025412.01.T01:intron 35.0%
! TCGTGTTCATTTAGCTTCAT+GGG + Chr5:18222553-18222572 None:intergenic 35.0%
! TGATTGCCATTTCTTTCTTG+AGG - Chr5:18223303-18223322 MsG0580025412.01.T01:intron 35.0%
! TGCTTTTCCACTTGGATTTT+AGG - Chr5:18222363-18222382 MsG0580025412.01.T01:intron 35.0%
! TTACTCTTTGGAAGAACTCA+AGG - Chr5:18220639-18220658 MsG0580025412.01.T01:three_prime_UTR 35.0%
! TTTTACACAAGATGCTCTGA+AGG - Chr5:18223445-18223464 MsG0580025412.01.T01:intron 35.0%
!! GACGGATATTCTGTTTTAAG+GGG + Chr5:18221419-18221438 None:intergenic 35.0%
!! TTGATCTTTGCTTTTCCACT+TGG - Chr5:18222355-18222374 MsG0580025412.01.T01:intron 35.0%
!!! CTGGCTTGCTTTTATTATGT+AGG - Chr5:18222461-18222480 MsG0580025412.01.T01:intron 35.0%
AAAGAGTTTACAAAGGGTGG+GGG + Chr5:18222326-18222345 None:intergenic 40.0%
AATGATCTCTGTCAGCTATG+TGG + Chr5:18221819-18221838 None:intergenic 40.0%
ACAAGACTCGTAGAATTAGG+AGG + Chr5:18223699-18223718 None:intergenic 40.0%
ACATTGAAGACATCGTTGCT+TGG + Chr5:18222046-18222065 None:intergenic 40.0%
ACGATGTCTTCAATGTCATC+TGG - Chr5:18222050-18222069 MsG0580025412.01.T01:intron 40.0%
AGAACTCAAGGTGATTACTG+AGG - Chr5:18220651-18220670 MsG0580025412.01.T01:three_prime_UTR 40.0%
AGATCAAGTGGTCATACATG+GGG - Chr5:18222846-18222865 MsG0580025412.01.T01:intron 40.0%
AGCACCATATTTCTCTCTCT+CGG + Chr5:18221351-18221370 None:intergenic 40.0%
ATACTGCAATGTTCCACTGT+CGG + Chr5:18220826-18220845 None:intergenic 40.0%
ATGATCTCTGTCAGCTATGT+GGG + Chr5:18221818-18221837 None:intergenic 40.0%
CAAATGCTGCTATGAAACAG+AGG + Chr5:18221852-18221871 None:intergenic 40.0%
CATCATCCCTAAAATCCAAG+TGG + Chr5:18222373-18222392 None:intergenic 40.0%
CCTAAGAAAGCTAGCAGATT+GGG + Chr5:18222516-18222535 None:intergenic 40.0%
CCTGTCTAAGTCATCACATA+AGG - Chr5:18222614-18222633 MsG0580025412.01.T01:intron 40.0%
CCTTATGTGATGACTTAGAC+AGG + Chr5:18222617-18222636 None:intergenic 40.0%
CTCTGCAATTCCAAAGAATG+AGG - Chr5:18221638-18221657 MsG0580025412.01.T01:intron 40.0%
GCAACAGAAAACAAGACCTA+TGG + Chr5:18222434-18222453 None:intergenic 40.0%
GCATTGCCTCAAGAAAGAAA+TGG + Chr5:18223312-18223331 None:intergenic 40.0%
GCGGGATTTGAAAGCAATTT+AGG - Chr5:18222894-18222913 MsG0580025412.01.T01:intron 40.0%
GGAGACAATGATAGATTGAC+GGG + Chr5:18222977-18222996 None:intergenic 40.0%
GTATAGGGAGAAAAGAGAGA+AGG + Chr5:18223180-18223199 None:intergenic 40.0%
TCAACCATATCCACAAGGTT+TGG + Chr5:18223636-18223655 None:intergenic 40.0%
TCATAGTTCCAATCCAGTCA+GGG + Chr5:18221570-18221589 None:intergenic 40.0%
TCCTAAGAAAGCTAGCAGAT+TGG + Chr5:18222517-18222536 None:intergenic 40.0%
TCCTAATTCTACGAGTCTTG+TGG - Chr5:18223698-18223717 MsG0580025412.01.T01:intron 40.0%
TGGTTTCTCACATGCTTTGA+GGG + Chr5:18221500-18221519 None:intergenic 40.0%
TTCAATCAAAAGATGGCGCA+AGG + Chr5:18223665-18223684 None:intergenic 40.0%
TTCACCGAGAGAGAGAAATA+TGG - Chr5:18221344-18221363 MsG0580025412.01.T01:intron 40.0%
TTCATAGTTCCAATCCAGTC+AGG + Chr5:18221571-18221590 None:intergenic 40.0%
TTCTAATATCATCCGGAGAG+CGG + Chr5:18222295-18222314 None:intergenic 40.0%
TTGGTTTCTCACATGCTTTG+AGG + Chr5:18221501-18221520 None:intergenic 40.0%
! AACTGTGTTTCTGTGGAACA+TGG + Chr5:18221900-18221919 None:intergenic 40.0%
! CAAATGTTTTTCGTTGGGTG+TGG + Chr5:18221947-18221966 None:intergenic 40.0%
! CCTAATTCTACGAGTCTTGT+GGG - Chr5:18223699-18223718 MsG0580025412.01.T01:intron 40.0%
! CTAAAATTTGGAACCCTGAC+TGG - Chr5:18221554-18221573 MsG0580025412.01.T01:intron 40.0%
! GTTTTCTGTTGCTCAGATTC+TGG - Chr5:18222442-18222461 MsG0580025412.01.T01:intron 40.0%
! TGGGATGTATGTTTTGTGAG+AGG - Chr5:18221269-18221288 MsG0580025412.01.T01:intron 40.0%
!! AGAGAGAAATATGGTGCTGT+AGG - Chr5:18221353-18221372 MsG0580025412.01.T01:intron 40.0%
!! CATTCTTTGGAATTGCAGAG+TGG + Chr5:18221638-18221657 None:intergenic 40.0%
!! TTGCGCCATCTTTTGATTGA+AGG - Chr5:18223664-18223683 MsG0580025412.01.T01:CDS 40.0%
AAATTTACCTTGAACCGCGG+AGG + Chr5:18220898-18220917 None:intergenic 45.0%
ACATAAGGCTCTTGAGAGCA+AGG - Chr5:18222629-18222648 MsG0580025412.01.T01:intron 45.0%
ACTAGGATACCTCTTGGCTT+TGG - Chr5:18221874-18221893 MsG0580025412.01.T01:intron 45.0%
AGTGGTCATACATGGGGTTT+AGG - Chr5:18222852-18222871 MsG0580025412.01.T01:intron 45.0%
CATTCAATCATGTGACAGCG+AGG - Chr5:18222098-18222117 MsG0580025412.01.T01:intron 45.0%
CCCACAAGACTCGTAGAATT+AGG + Chr5:18223702-18223721 None:intergenic 45.0%
CGGGCTAAAATAGGATTAGG+CGG - Chr5:18222875-18222894 MsG0580025412.01.T01:intron 45.0%
GACAGTGGAACATTGCAGTA+TGG - Chr5:18220825-18220844 MsG0580025412.01.T01:exon 45.0%
GGGAAAATGGTGTCTTGAGA+AGG - Chr5:18222765-18222784 MsG0580025412.01.T01:intron 45.0%
GGGCTAAAATAGGATTAGGC+GGG - Chr5:18222876-18222895 MsG0580025412.01.T01:intron 45.0%
GTAGCTGAGAAATCAACTCG+AGG - Chr5:18222123-18222142 MsG0580025412.01.T01:intron 45.0%
GTTGAACGACTACTGGCATA+AGG + Chr5:18223735-18223754 None:intergenic 45.0%
TATGGAAGATGTGCAGTGGA+TGG - Chr5:18222795-18222814 MsG0580025412.01.T01:intron 45.0%
TCATAGCAGCATTTGCCACT+AGG - Chr5:18221857-18221876 MsG0580025412.01.T01:intron 45.0%
TCCAAAGAGTAAGGATGCTC+AGG + Chr5:18220631-18220650 None:intergenic 45.0%
TCCTGAGCATCCTTACTCTT+TGG - Chr5:18220627-18220646 MsG0580025412.01.T01:three_prime_UTR 45.0%
TCTAATATCATCCGGAGAGC+GGG + Chr5:18222294-18222313 None:intergenic 45.0%
TGAAGACATCGTTGCTTGGA+AGG + Chr5:18222042-18222061 None:intergenic 45.0%
TTTGCCACTAGGATACCTCT+TGG - Chr5:18221868-18221887 MsG0580025412.01.T01:intron 45.0%
! AAGCTAGTTTCCCTACTGGA+GGG - Chr5:18222229-18222248 MsG0580025412.01.T01:intron 45.0%
! AGAAATGAAGAGGCATGGAC+AGG - Chr5:18222716-18222735 MsG0580025412.01.T01:intron 45.0%
! AGGGTTTGAGTTTCAGCTCA+TGG + Chr5:18221481-18221500 None:intergenic 45.0%
! ATTTGGAACCCTGACTGGAT+TGG - Chr5:18221559-18221578 MsG0580025412.01.T01:intron 45.0%
! GGGTTTGAGTTTCAGCTCAT+GGG + Chr5:18221480-18221499 None:intergenic 45.0%
! TTTTTCGTTGGGTGTGGAAG+TGG + Chr5:18221941-18221960 None:intergenic 45.0%
!! AAAGCCAAGAGGTATCCTAG+TGG + Chr5:18221875-18221894 None:intergenic 45.0%
!! ACTGCACCCATGTGTTTTAG+AGG - Chr5:18223210-18223229 MsG0580025412.01.T01:intron 45.0%
!! AGGCGGGCTAAAATAGGATT+AGG - Chr5:18222872-18222891 MsG0580025412.01.T01:intron 45.0%
!! AGTTGAAAAGGCACCATCTG+AGG - Chr5:18222013-18222032 MsG0580025412.01.T01:intron 45.0%
!! CCCAATCTGCTAGCTTTCTT+AGG - Chr5:18222513-18222532 MsG0580025412.01.T01:intron 45.0%
AGTGGATGGCGAAAACGAAG+GGG - Chr5:18222809-18222828 MsG0580025412.01.T01:intron 50.0%
AGTTTACAAAGGGTGGGGGA+AGG + Chr5:18222322-18222341 None:intergenic 50.0%
ATGGTGTCTTGAGAAGGAGC+TGG - Chr5:18222771-18222790 MsG0580025412.01.T01:intron 50.0%
CAGTGGATGGCGAAAACGAA+GGG - Chr5:18222808-18222827 MsG0580025412.01.T01:intron 50.0%
GCACTAAGGTCAGAGCAGTT+TGG + Chr5:18221755-18221774 None:intergenic 50.0%
GCTTCAGTTGCATGAGATCC+AGG + Chr5:18223137-18223156 None:intergenic 50.0%
GGTCATACATGGGGTTTAGG+CGG - Chr5:18222855-18222874 MsG0580025412.01.T01:intron 50.0%
GGTTACGTTTACTCCGACAG+TGG - Chr5:18220810-18220829 MsG0580025412.01.T01:three_prime_UTR 50.0%
GTCATACATGGGGTTTAGGC+GGG - Chr5:18222856-18222875 MsG0580025412.01.T01:intron 50.0%
TCAATCATGTGACAGCGAGG+AGG - Chr5:18222101-18222120 MsG0580025412.01.T01:intron 50.0%
TCTCATGCAACTGAAGCTGC+AGG - Chr5:18223140-18223159 MsG0580025412.01.T01:intron 50.0%
TCTTGAGAAGGAGCTGGCTA+TGG - Chr5:18222777-18222796 MsG0580025412.01.T01:intron 50.0%
TCTTGAGAAGTTTGCCTCCG+CGG - Chr5:18220881-18220900 MsG0580025412.01.T01:CDS 50.0%
TGGCTATGGAAGATGTGCAG+TGG - Chr5:18222791-18222810 MsG0580025412.01.T01:intron 50.0%
TGGTTAGGTGGACATCAGAG+TGG - Chr5:18223109-18223128 MsG0580025412.01.T01:intron 50.0%
TTGCTTCAGGGACTGCACTA+AGG + Chr5:18221769-18221788 None:intergenic 50.0%
TTTCTCCATTACCCGCTCTC+CGG - Chr5:18222280-18222299 MsG0580025412.01.T01:intron 50.0%
! CAAGCTAGTTTCCCTACTGG+AGG - Chr5:18222228-18222247 MsG0580025412.01.T01:intron 50.0%
! CAGCAAGCTAGTTTCCCTAC+TGG - Chr5:18222225-18222244 MsG0580025412.01.T01:intron 50.0%
! TCAGCTCATGGGTTGCTTGA+AGG + Chr5:18221469-18221488 None:intergenic 50.0%
!! GAGCTTTGAAACCCCTATGC+TGG - Chr5:18223056-18223075 MsG0580025412.01.T01:intron 50.0%
!! GGGTTTAGGCGGGCTAAAAT+AGG - Chr5:18222866-18222885 MsG0580025412.01.T01:intron 50.0%
AAGTTTGCCTCCGCGGTTCA+AGG - Chr5:18220888-18220907 MsG0580025412.01.T01:CDS 55.0%
ACCCTTGGATCCCTCCAGTA+GGG + Chr5:18222242-18222261 None:intergenic 55.0%
ATCATCCGGAGAGCGGGTAA+TGG + Chr5:18222288-18222307 None:intergenic 55.0%
CTCTGTCAGCTATGTGGGCT+AGG + Chr5:18221813-18221832 None:intergenic 55.0%
GCAGTGGATGGCGAAAACGA+AGG - Chr5:18222807-18222826 MsG0580025412.01.T01:intron 55.0%
GGAACATGGCCAAAGCCAAG+AGG + Chr5:18221886-18221905 None:intergenic 55.0%
TCCCTACTGGAGGGATCCAA+GGG - Chr5:18222238-18222257 MsG0580025412.01.T01:intron 55.0%
TGCACGGTGCTTAACTGCTC+TGG - Chr5:18221184-18221203 MsG0580025412.01.T01:intron 55.0%
TTCCCTACTGGAGGGATCCA+AGG - Chr5:18222237-18222256 MsG0580025412.01.T01:intron 55.0%
GACCCTTGGATCCCTCCAGT+AGG + Chr5:18222243-18222262 None:intergenic 60.0%
GCTTGGAAGGCAGCCTCAGA+TGG + Chr5:18222029-18222048 None:intergenic 60.0%
GTGGACATCAGAGTGGCACC+TGG - Chr5:18223116-18223135 MsG0580025412.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 18220479 18224039 18220479 ID=MsG0580025412.01;Name=MsG0580025412.01
Chr5 mRNA 18220479 18224039 18220479 ID=MsG0580025412.01.T01;Parent=MsG0580025412.01;Name=MsG0580025412.01.T01;_AED=0.41;_eAED=0.44;_QI=0|0.75|0.6|1|1|1|5|356|131
Chr5 exon 18220479 18220935 18220479 ID=MsG0580025412.01.T01:exon:2011;Parent=MsG0580025412.01.T01
Chr5 exon 18221357 18221402 18221357 ID=MsG0580025412.01.T01:exon:2010;Parent=MsG0580025412.01.T01
Chr5 exon 18223609 18223708 18223609 ID=MsG0580025412.01.T01:exon:2009;Parent=MsG0580025412.01.T01
Chr5 exon 18223808 18223901 18223808 ID=MsG0580025412.01.T01:exon:2008;Parent=MsG0580025412.01.T01
Chr5 exon 18223985 18224039 18223985 ID=MsG0580025412.01.T01:exon:2007;Parent=MsG0580025412.01.T01
Chr5 CDS 18223985 18224039 18223985 ID=MsG0580025412.01.T01:cds;Parent=MsG0580025412.01.T01
Chr5 CDS 18223808 18223901 18223808 ID=MsG0580025412.01.T01:cds;Parent=MsG0580025412.01.T01
Chr5 CDS 18223609 18223708 18223609 ID=MsG0580025412.01.T01:cds;Parent=MsG0580025412.01.T01
Chr5 CDS 18221357 18221402 18221357 ID=MsG0580025412.01.T01:cds;Parent=MsG0580025412.01.T01
Chr5 CDS 18220835 18220935 18220835 ID=MsG0580025412.01.T01:cds;Parent=MsG0580025412.01.T01
Chr5 three_prime_UTR 18220479 18220834 18220479 ID=MsG0580025412.01.T01:three_prime_utr;Parent=MsG0580025412.01.T01
Gene Sequence

>MsG0580025412.01.T01

ATGGGCCCTTTATGTTTTGTTTTAGAAATAGCTTATCATGATAGTTGTTTTTTCTATATCAAGGATCCTGAGCATCCTTACTCTTTGGAAGAACTCAAGGTGATTACTGAGGAAGCTGTCGAAGTCGATGATCAGAAAAGTTATGTCAGGGTTACGTTTACTCCGACAGTGGAACATTGCAGTATGGCAACAATTATAGGTCTTTGTTTACGGGTCAAGCTCTTGAGAAGTTTGCCTCCGCGGTTCAAGGTGGACATCAGAGTGGCACCTGGATCTCATGCAACTGAAGCTGCAGTTAACAAACAACTAAATGATAAAGAGCGTGTAGCTGCTGCACTTGAAAATCCAAACCTTGTGGATATGGTTGATGAATGCCTTGCGCCATCTTTTGATTGA
Protein sequence

>MsG0580025412.01.T01

MGPLCFVLEIAYHDSCFFYIKDPEHPYSLEELKVITEEAVEVDDQKSYVRVTFTPTVEHCSMATIIGLCLRVKLLRSLPPRFKVDIRVAPGSHATEAAVNKQLNDKERVAAALENPNLVDMVDECLAPSFD*