AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025934.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025934.01.T01 MTR_7g108340 94.048 84 5 0 13 96 494 577 4.65e-48 168
MsG0580025934.01.T01 MTR_2g437390 82.022 89 16 0 8 96 496 584 1.41e-42 153
MsG0580025934.01.T01 MTR_4g119413 83.333 84 14 0 16 99 506 589 1.36e-41 149
MsG0580025934.01.T01 MTR_4g119413 80.952 84 16 0 13 96 503 586 9.57e-41 147
MsG0580025934.01.T01 MTR_3g077910 93.750 64 4 0 162 225 235 298 8.47e-36 128
MsG0580025934.01.T01 MTR_3g077930 91.379 58 5 0 162 219 137 194 3.46e-31 113
MsG0580025934.01.T01 MTR_5g090900 86.154 65 9 0 92 156 90 154 2.03e-30 117
MsG0580025934.01.T01 MTR_3g077940 87.273 55 7 0 162 216 164 218 1.96e-28 107
MsG0580025934.01.T01 MTR_3g077940 87.273 55 7 0 162 216 203 257 2.61e-28 107
MsG0580025934.01.T01 MTR_3g450760 62.500 64 24 0 92 155 78 141 1.91e-19 86.3
MsG0580025934.01.T01 MTR_2g019260 40.789 76 45 0 17 92 553 628 5.87e-11 62.0
MsG0580025934.01.T01 MTR_1g040925 39.000 100 54 2 1 93 675 774 6.61e-11 62.0
MsG0580025934.01.T01 MTR_2g019260 40.789 76 45 0 17 92 553 628 6.65e-11 62.0
MsG0580025934.01.T01 MTR_1g040995 39.000 100 54 2 1 93 691 790 8.33e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025934.01.T01 AT4G02570 84.146 82 13 0 15 96 499 580 5.62e-42 151
MsG0580025934.01.T01 AT4G02570 84.146 82 13 0 15 96 499 580 5.62e-42 151
MsG0580025934.01.T01 AT4G02570 84.146 82 13 0 15 96 499 580 5.62e-42 151
MsG0580025934.01.T01 AT4G02570 84.146 82 13 0 15 96 499 580 5.62e-42 151
MsG0580025934.01.T01 AT1G43140 72.500 80 22 0 17 96 484 563 7.33e-33 125
MsG0580025934.01.T01 AT1G02980 66.250 80 27 0 17 96 505 584 1.97e-30 118
MsG0580025934.01.T01 AT1G02980 66.250 80 27 0 17 96 505 584 1.97e-30 118
MsG0580025934.01.T01 AT5G46210 40.260 77 46 0 17 93 553 629 3.13e-11 62.8

Find 56 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCTTTGAGGTTACATTCT+TGG 0.158322 5:-27411843 MsG0580025934.01.T01:CDS
CATAGTAAGTTCATTGTTAA+TGG 0.192416 5:+27411759 None:intergenic
CCTATTTGCGATAGTGATTC+TGG 0.263591 5:-27411865 MsG0580025934.01.T01:CDS
GATATTAAGTTGTTAAATAT+TGG 0.270704 5:+27410766 None:intergenic
CCAGAATCACTATCGCAAAT+AGG 0.272055 5:+27411865 None:intergenic
CATTAACAATGAACTTACTA+TGG 0.283575 5:-27411758 MsG0580025934.01.T01:intron
CTGTTCTTACCACTGGGTTT+TGG 0.289451 5:-27412759 MsG0580025934.01.T01:CDS
TGCCCTTTGAAGGGGCAATT+TGG 0.290512 5:-27414456 MsG0580025934.01.T01:intron
TTTAACAACTTAATATCTAA+TGG 0.303877 5:-27410760 MsG0580025934.01.T01:three_prime_UTR
CAACATATGGATATGTCTTC+AGG 0.322130 5:+27411105 None:intergenic
CTTATTTCTCTATCAACATA+TGG 0.332603 5:+27411092 None:intergenic
CAGAATCACTATCGCAAATA+GGG 0.338443 5:+27411866 None:intergenic
ACAGTTACTGTTCTTACCAC+TGG 0.345116 5:-27412766 MsG0580025934.01.T01:CDS
AGTTCATTGTTAATGGGAAT+TGG 0.368214 5:+27411766 None:intergenic
ATAGTAAGTTCATTGTTAAT+GGG 0.372091 5:+27411760 None:intergenic
GTTAATTGTCTCAACCTATC+AGG 0.377524 5:-27412467 MsG0580025934.01.T01:intron
AGTTTCTCAACAGATGTATT+GGG 0.391020 5:+27411817 None:intergenic
ATAAAATTATTCAAGCTTTG+TGG 0.399269 5:+27410793 None:intergenic
CCTGTTACCATCCCTCTAAA+AGG 0.407387 5:-27410981 MsG0580025934.01.T01:CDS
AAAGCTTGTGGTACTTTAGC+AGG 0.410486 5:-27411934 MsG0580025934.01.T01:CDS
CATATGTTGATAGAGAAATA+AGG 0.418585 5:-27411091 MsG0580025934.01.T01:CDS
GAGTTTCTCAACAGATGTAT+TGG 0.419693 5:+27411816 None:intergenic
GTCAAATTTCTTAAAGCTTG+TGG 0.426131 5:-27411946 MsG0580025934.01.T01:CDS
GAACAAGAACTTAGCACTAC+TGG 0.433343 5:-27411069 MsG0580025934.01.T01:CDS
CTGGGAACTTGCAACATCAT+TGG 0.433672 5:-27412514 MsG0580025934.01.T01:CDS
CAGTTACTGTTCTTACCACT+GGG 0.453302 5:-27412765 MsG0580025934.01.T01:CDS
ATGGGAATTGGTGGATCAAC+TGG 0.454733 5:+27411778 None:intergenic
GTAACTGTCAAGTCAATTCC+AGG 0.457681 5:+27412781 None:intergenic
GCCAAGAATGTAACCTCAAA+GGG 0.461712 5:+27411842 None:intergenic
ACTGACATTGATTTACTCAC+TGG 0.478913 5:-27412533 MsG0580025934.01.T01:CDS
TACCAAATTGCCCCTTCAAA+GGG 0.495540 5:+27414454 None:intergenic
TTACCAAATTGCCCCTTCAA+AGG 0.499342 5:+27414453 None:intergenic
GAAAACTCAGATGTACATCC+TGG 0.500576 5:-27412799 MsG0580025934.01.T01:intron
ATAGTGATTCTGGCCCTTTG+AGG 0.503013 5:-27411855 MsG0580025934.01.T01:CDS
TCATTGTTAATGGGAATTGG+TGG 0.511207 5:+27411769 None:intergenic
CAACTGGTTGGACATTCAGC+TGG 0.522362 5:+27411794 None:intergenic
CAGGAAGGGAATCACCTGAT+AGG 0.523095 5:+27412453 None:intergenic
TGTGTAAATTATTACAGATG+AGG 0.532464 5:-27410844 MsG0580025934.01.T01:three_prime_UTR
CTCCTACCATCTCAGAAGGC+AGG 0.538416 5:+27412710 None:intergenic
CTGACATTGATTTACTCACT+GGG 0.543719 5:-27412532 MsG0580025934.01.T01:CDS
AGATCAAAAGACTTGTAGCT+TGG 0.549941 5:+27412736 None:intergenic
AGCCAAGAATGTAACCTCAA+AGG 0.554321 5:+27411841 None:intergenic
AACAAGAACTTAGCACTACT+GGG 0.566092 5:-27411068 MsG0580025934.01.T01:CDS
GGTTGAGACAATTAACTCGA+TGG 0.569225 5:+27412474 None:intergenic
CATAACTATCTAAATCCTCA+AGG 0.569507 5:-27410952 MsG0580025934.01.T01:exon
TCTGAACCTGCCTTCTGAGA+TGG 0.574542 5:-27412716 MsG0580025934.01.T01:intron
GGATTTAGATAGTTATGTCA+AGG 0.575998 5:+27410958 None:intergenic
GTTCAAAGCTTGATTCCTTG+AGG 0.576009 5:+27410937 None:intergenic
GAATTGGTGGATCAACTGGT+TGG 0.601820 5:+27411782 None:intergenic
TATGCTCCTACCATCTCAGA+AGG 0.602920 5:+27412706 None:intergenic
TGCTGAAATACGCAAAACAT+CGG 0.610069 5:-27411905 MsG0580025934.01.T01:CDS
TGAATGGAGAATTCAAGTTG+TGG 0.619747 5:-27411028 MsG0580025934.01.T01:CDS
AGAATCACTATCGCAAATAG+GGG 0.636968 5:+27411867 None:intergenic
ACAACGATATACAATGACCA+AGG 0.706830 5:+27410882 None:intergenic
GTAGCTTGGCCAAAACCCAG+TGG 0.742379 5:+27412750 None:intergenic
AAGAACTTAGCACTACTGGG+AGG 0.768897 5:-27411065 MsG0580025934.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTAAATATTTGAAGTA+TGG + Chr5:27414015-27414034 None:intergenic 10.0%
!!! GTTAATATTTACAATTTTTA+TGG - Chr5:27413811-27413830 MsG0580025934.01.T01:intron 10.0%
!!! TTTTATTATCTTTTTTTCAA+AGG - Chr5:27411290-27411309 MsG0580025934.01.T01:intron 10.0%
!! AAAAATCTTCCTTTAATATA+AGG - Chr5:27412095-27412114 MsG0580025934.01.T01:intron 15.0%
!! GATAATAAAATTAAATGGAA+CGG + Chr5:27411280-27411299 None:intergenic 15.0%
!! TTTAACAACTTAATATCTAA+TGG - Chr5:27414433-27414452 MsG0580025934.01.T01:intron 15.0%
!!! ATCAATTGTTTTTTTTTTTC+AGG - Chr5:27412582-27412601 MsG0580025934.01.T01:CDS 15.0%
!!! GATATTAAGTTGTTAAATAT+TGG + Chr5:27414430-27414449 None:intergenic 15.0%
!!! TAATTTGAGTTGTTTAATTT+TGG + Chr5:27411040-27411059 None:intergenic 15.0%
!!! TTATAAGAATTCCATATTTT+TGG + Chr5:27411766-27411785 None:intergenic 15.0%
!!! TTTTAATGCATAAAATTGAA+TGG - Chr5:27411084-27411103 MsG0580025934.01.T01:CDS 15.0%
!! AAAACAACATATATATGTTG+AGG + Chr5:27413152-27413171 None:intergenic 20.0%
!! AACAAAACACCTTATATTAA+AGG + Chr5:27412107-27412126 None:intergenic 20.0%
!! AGAATCATGAAGAAAAAAAA+AGG + Chr5:27411986-27412005 None:intergenic 20.0%
!! ATAAAATTATTCAAGCTTTG+TGG + Chr5:27414403-27414422 None:intergenic 20.0%
!! ATAGTAAGTTCATTGTTAAT+GGG + Chr5:27413436-27413455 None:intergenic 20.0%
!! CATGAATTATACATAATACT+AGG - Chr5:27413775-27413794 MsG0580025934.01.T01:intron 20.0%
!! GTATAATTCATGTATTACTA+TGG + Chr5:27413767-27413786 None:intergenic 20.0%
!! TAATGATAATTATTTGGATG+AGG + Chr5:27410797-27410816 None:intergenic 20.0%
!! TGAATGTGAGAAATAATTTA+AGG + Chr5:27411865-27411884 None:intergenic 20.0%
!! TTTCTGATTGTAGTTATTTA+AGG - Chr5:27412510-27412529 MsG0580025934.01.T01:CDS 20.0%
!! TTTGAAAGTTACCAAAAATA+TGG - Chr5:27411752-27411771 MsG0580025934.01.T01:intron 20.0%
! AATCGAATAATGACTAATCT+AGG - Chr5:27413044-27413063 MsG0580025934.01.T01:intron 25.0%
! AATGTATCAAATGCAAAAAC+AGG + Chr5:27412209-27412228 None:intergenic 25.0%
! ATGTGAGAAATAATTTAAGG+AGG + Chr5:27411862-27411881 None:intergenic 25.0%
! ATTCAAATATTCCTTAACCT+TGG - Chr5:27410762-27410781 MsG0580025934.01.T01:three_prime_UTR 25.0%
! CATAGTAAGTTCATTGTTAA+TGG + Chr5:27413437-27413456 None:intergenic 25.0%
! CATTAACAATGAACTTACTA+TGG - Chr5:27413435-27413454 MsG0580025934.01.T01:intron 25.0%
! CCTTAAAAATGTAGAGAATT+TGG + Chr5:27410986-27411005 None:intergenic 25.0%
! CGTGAATAAAAATATTTGTG+AGG + Chr5:27411375-27411394 None:intergenic 25.0%
! GACTTCTAACATTTCAAAAT+TGG + Chr5:27413093-27413112 None:intergenic 25.0%
! TAGAGAATTTGGAAAAAAGT+CGG + Chr5:27410975-27410994 None:intergenic 25.0%
! TGATTCCTGTATATATAAAG+TGG + Chr5:27414048-27414067 None:intergenic 25.0%
! TGTGTAAATTATTACAGATG+AGG - Chr5:27414349-27414368 MsG0580025934.01.T01:intron 25.0%
! TTCTGACGAAATTGTATTAT+GGG - Chr5:27412332-27412351 MsG0580025934.01.T01:intron 25.0%
!! CATAGTTTGTCACAATATAA+AGG + Chr5:27411636-27411655 None:intergenic 25.0%
!! CATATGTTGATAGAGAAATA+AGG - Chr5:27414102-27414121 MsG0580025934.01.T01:intron 25.0%
!! CATTATATTTGCTACATTGT+TGG + Chr5:27411708-27411727 None:intergenic 25.0%
!! CCAAATTCTCTACATTTTTA+AGG - Chr5:27410983-27411002 MsG0580025934.01.T01:CDS 25.0%
!! CTTATTTCTCTATCAACATA+TGG + Chr5:27414104-27414123 None:intergenic 25.0%
!! GTTTTCATTTGAATCTTGTA+AGG + Chr5:27413024-27413043 None:intergenic 25.0%
!! TGTCATGATGATTGAATTTT+AGG - Chr5:27412274-27412293 MsG0580025934.01.T01:intron 25.0%
!! TTCATGATTCTGCATTTTAA+GGG - Chr5:27411995-27412014 MsG0580025934.01.T01:intron 25.0%
!!! AGATGGTTTTTTTTTAGTTG+AGG - Chr5:27412153-27412172 MsG0580025934.01.T01:intron 25.0%
!!! CATTTTGATTTCATTTTGAG+GGG - Chr5:27411911-27411930 MsG0580025934.01.T01:CDS 25.0%
!!! CTATTGTGTCATGTTTTATT+AGG - Chr5:27413117-27413136 MsG0580025934.01.T01:intron 25.0%
!!! CTTTTTCAGAAACTTGATTT+TGG + Chr5:27413896-27413915 None:intergenic 25.0%
!!! GCATTTTGATTTCATTTTGA+GGG - Chr5:27411910-27411929 MsG0580025934.01.T01:CDS 25.0%
!!! TACATTTTTAAGGAGAAGTT+AGG - Chr5:27410993-27411012 MsG0580025934.01.T01:CDS 25.0%
!!! TGCATTTTGATTTCATTTTG+AGG - Chr5:27411909-27411928 MsG0580025934.01.T01:CDS 25.0%
AACAACCCATGTTAATATCA+AGG - Chr5:27413711-27413730 MsG0580025934.01.T01:intron 30.0%
ACAATTCGAATTCTGTGAAA+TGG - Chr5:27413941-27413960 MsG0580025934.01.T01:intron 30.0%
AGAGAATTTGGAAAAAAGTC+GGG + Chr5:27410974-27410993 None:intergenic 30.0%
AGTTCATTGTTAATGGGAAT+TGG + Chr5:27413430-27413449 None:intergenic 30.0%
AGTTTCTCAACAGATGTATT+GGG + Chr5:27413379-27413398 None:intergenic 30.0%
ATGAGCCTTGATATTAACAT+GGG + Chr5:27413719-27413738 None:intergenic 30.0%
ATTCGAAAATTCTCTTCTAC+AGG - Chr5:27412815-27412834 MsG0580025934.01.T01:intron 30.0%
CATAACTATCTAAATCCTCA+AGG - Chr5:27414241-27414260 MsG0580025934.01.T01:intron 30.0%
CTTCTGACGAAATTGTATTA+TGG - Chr5:27412331-27412350 MsG0580025934.01.T01:intron 30.0%
GAAGAAATGAAGATGAACTT+AGG - Chr5:27413664-27413683 MsG0580025934.01.T01:intron 30.0%
GAGAAAAGAAAGCTAAGTTA+GGG + Chr5:27413071-27413090 None:intergenic 30.0%
GCATAACAACATGACATAAA+AGG + Chr5:27412774-27412793 None:intergenic 30.0%
GGATTTAGATAGTTATGTCA+AGG + Chr5:27414238-27414257 None:intergenic 30.0%
GTACATAATCCACTAACAAT+CGG + Chr5:27412047-27412066 None:intergenic 30.0%
GTCAAATTTCTTAAAGCTTG+TGG - Chr5:27413247-27413266 MsG0580025934.01.T01:intron 30.0%
GTTTAGAAGCAACTGAATTT+AGG + Chr5:27410920-27410939 None:intergenic 30.0%
TACATAATCCACTAACAATC+GGG + Chr5:27412046-27412065 None:intergenic 30.0%
TCGCGTAAGAAAAAAATTAG+AGG + Chr5:27413509-27413528 None:intergenic 30.0%
TCTCACATTGTAGGAAAAAA+TGG - Chr5:27413620-27413639 MsG0580025934.01.T01:intron 30.0%
TGATAATTATTTGGATGAGG+TGG + Chr5:27410794-27410813 None:intergenic 30.0%
! AAGTGTGTCGAAGTTTTTAA+GGG - Chr5:27412607-27412626 MsG0580025934.01.T01:intron 30.0%
! ACAATTAACTCGATGGTTTT+TGG + Chr5:27412715-27412734 None:intergenic 30.0%
! AGATAGCATTCAACAACTTT+TGG + Chr5:27411122-27411141 None:intergenic 30.0%
! AGTTTTCTCTGAGTTTTTCA+CGG - Chr5:27412904-27412923 MsG0580025934.01.T01:intron 30.0%
! CTTCATGATTCTGCATTTTA+AGG - Chr5:27411994-27412013 MsG0580025934.01.T01:intron 30.0%
! GGTATATATCATGTGAGTTT+TGG - Chr5:27412942-27412961 MsG0580025934.01.T01:intron 30.0%
! GGTTACTATGATTTTGTGAA+TGG - Chr5:27414149-27414168 MsG0580025934.01.T01:intron 30.0%
! TATGTCATCTCTTTCTGTTT+TGG - Chr5:27413552-27413571 MsG0580025934.01.T01:intron 30.0%
! TTCTAAGTTACTCATTTTGC+AGG - Chr5:27413225-27413244 MsG0580025934.01.T01:intron 30.0%
! TTTAGTTTCTGTTTCATCAG+AGG - Chr5:27411950-27411969 MsG0580025934.01.T01:intron 30.0%
! TTTCAATTTTGCCCTTTGAA+GGG - Chr5:27410728-27410747 MsG0580025934.01.T01:three_prime_UTR 30.0%
! TTTTCAATTTTGCCCTTTGA+AGG - Chr5:27410727-27410746 MsG0580025934.01.T01:three_prime_UTR 30.0%
!! AGGTGTTTTGTTCTTCATTT+TGG - Chr5:27412115-27412134 MsG0580025934.01.T01:intron 30.0%
!! GGGTTTATTTTCAAGAAAGA+TGG - Chr5:27412136-27412155 MsG0580025934.01.T01:intron 30.0%
!! GGTGTTTTGTTCTTCATTTT+GGG - Chr5:27412116-27412135 MsG0580025934.01.T01:intron 30.0%
!!! TTTTTTTTCCCGATTGTTAG+TGG - Chr5:27412035-27412054 MsG0580025934.01.T01:intron 30.0%
AACAAGAACTTAGCACTACT+GGG - Chr5:27414125-27414144 MsG0580025934.01.T01:intron 35.0%
ACAACGATATACAATGACCA+AGG + Chr5:27414314-27414333 None:intergenic 35.0%
AGAATCACTATCGCAAATAG+GGG + Chr5:27413329-27413348 None:intergenic 35.0%
AGATCAAAAGACTTGTAGCT+TGG + Chr5:27412460-27412479 None:intergenic 35.0%
AGGCACCACTTTATATATAC+AGG - Chr5:27414040-27414059 MsG0580025934.01.T01:intron 35.0%
ATAGATGATCGGGTGAAAAT+TGG - Chr5:27413862-27413881 MsG0580025934.01.T01:intron 35.0%
CAACATATGGATATGTCTTC+AGG + Chr5:27414091-27414110 None:intergenic 35.0%
CAAGTGACATAGAAAAACCT+TGG - Chr5:27414294-27414313 MsG0580025934.01.T01:intron 35.0%
CACATTGTAGGAAAAAATGG+AGG - Chr5:27413623-27413642 MsG0580025934.01.T01:intron 35.0%
CAGAATCACTATCGCAAATA+GGG + Chr5:27413330-27413349 None:intergenic 35.0%
CATGAGCCTTGATATTAACA+TGG + Chr5:27413720-27413739 None:intergenic 35.0%
GAGTTTCTCAACAGATGTAT+TGG + Chr5:27413380-27413399 None:intergenic 35.0%
GCATGCATTTATCTAGACTA+TGG + Chr5:27412237-27412256 None:intergenic 35.0%
GGAGAAAAGAAAGCTAAGTT+AGG + Chr5:27413072-27413091 None:intergenic 35.0%
GTCATGCAACATAACAATCT+CGG + Chr5:27412070-27412089 None:intergenic 35.0%
GTTAATTGTCTCAACCTATC+AGG - Chr5:27412726-27412745 MsG0580025934.01.T01:CDS 35.0%
TCATGCAACATAACAATCTC+GGG + Chr5:27412069-27412088 None:intergenic 35.0%
TCATTGTTAATGGGAATTGG+TGG + Chr5:27413427-27413446 None:intergenic 35.0%
TCTAGATAAATGCATGCATG+TGG - Chr5:27412240-27412259 MsG0580025934.01.T01:intron 35.0%
TGCGGATAAGCTGAATTATT+CGG - Chr5:27412863-27412882 MsG0580025934.01.T01:intron 35.0%
TGCTGAAATACGCAAAACAT+CGG - Chr5:27413288-27413307 MsG0580025934.01.T01:intron 35.0%
! CAAGTGTGTCGAAGTTTTTA+AGG - Chr5:27412606-27412625 MsG0580025934.01.T01:intron 35.0%
! CATTGTTGTTCTCACATTGT+AGG - Chr5:27413611-27413630 MsG0580025934.01.T01:intron 35.0%
! TTATGTCAAGGCCTTTTAGA+GGG + Chr5:27414226-27414245 None:intergenic 35.0%
!! ACTGACATTGATTTACTCAC+TGG - Chr5:27412660-27412679 MsG0580025934.01.T01:intron 35.0%
!! CTGACATTGATTTACTCACT+GGG - Chr5:27412661-27412680 MsG0580025934.01.T01:intron 35.0%
!! GGCTCTAGTATCAAACATTT+TGG + Chr5:27411019-27411038 None:intergenic 35.0%
!! TGAATGGAGAATTCAAGTTG+TGG - Chr5:27414165-27414184 MsG0580025934.01.T01:intron 35.0%
!! TTCAATTTTGCCCTTTGAAG+GGG - Chr5:27410729-27410748 MsG0580025934.01.T01:three_prime_UTR 35.0%
!!! CTGTTTTGGTATATTCGAGA+TGG - Chr5:27413566-27413585 MsG0580025934.01.T01:intron 35.0%
AAAAGGATGAGTCTCAGGAA+GGG + Chr5:27412757-27412776 None:intergenic 40.0%
AAAGCTTGTGGTACTTTAGC+AGG - Chr5:27413259-27413278 MsG0580025934.01.T01:intron 40.0%
ACAGTTACTGTTCTTACCAC+TGG - Chr5:27412427-27412446 MsG0580025934.01.T01:intron 40.0%
ACCTCTCTTCAACTCATGTT+GGG - Chr5:27411176-27411195 MsG0580025934.01.T01:intron 40.0%
AGCCAAGAATGTAACCTCAA+AGG + Chr5:27413355-27413374 None:intergenic 40.0%
AGTTTCTGTTTCATCAGAGG+TGG - Chr5:27411953-27411972 MsG0580025934.01.T01:intron 40.0%
CAGTGACTTCATAGATGATC+GGG - Chr5:27413852-27413871 MsG0580025934.01.T01:intron 40.0%
CAGTTACTGTTCTTACCACT+GGG - Chr5:27412428-27412447 MsG0580025934.01.T01:intron 40.0%
CCAGAATCACTATCGCAAAT+AGG + Chr5:27413331-27413350 None:intergenic 40.0%
CCTATTTGCGATAGTGATTC+TGG - Chr5:27413328-27413347 MsG0580025934.01.T01:intron 40.0%
GAAAACTCAGATGTACATCC+TGG - Chr5:27412394-27412413 MsG0580025934.01.T01:intron 40.0%
GAACAAGAACTTAGCACTAC+TGG - Chr5:27414124-27414143 MsG0580025934.01.T01:intron 40.0%
GACATAAAAGGATGAGTCTC+AGG + Chr5:27412762-27412781 None:intergenic 40.0%
GCAGTGACTTCATAGATGAT+CGG - Chr5:27413851-27413870 MsG0580025934.01.T01:intron 40.0%
GCCAAGAATGTAACCTCAAA+GGG + Chr5:27413354-27413373 None:intergenic 40.0%
GGTGTCTTAATCTTCCACAA+CGG + Chr5:27410945-27410964 None:intergenic 40.0%
GGTTGAGACAATTAACTCGA+TGG + Chr5:27412722-27412741 None:intergenic 40.0%
GTAACTGTCAAGTCAATTCC+AGG + Chr5:27412415-27412434 None:intergenic 40.0%
GTTCAAAGCTTGATTCCTTG+AGG + Chr5:27414259-27414278 None:intergenic 40.0%
TAAAAGGATGAGTCTCAGGA+AGG + Chr5:27412758-27412777 None:intergenic 40.0%
TACCAAATTGCCCCTTCAAA+GGG + Chr5:27410742-27410761 None:intergenic 40.0%
TCTTCGTCAGAGAACACTAA+TGG + Chr5:27412538-27412557 None:intergenic 40.0%
TGGAAAAAAGTCGGGTCAAA+CGG + Chr5:27410966-27410985 None:intergenic 40.0%
TGTTGCATAGATACAACCTC+AGG + Chr5:27413185-27413204 None:intergenic 40.0%
TTACCAAATTGCCCCTTCAA+AGG + Chr5:27410743-27410762 None:intergenic 40.0%
! GTTATGTCAAGGCCTTTTAG+AGG + Chr5:27414227-27414246 None:intergenic 40.0%
! TCACGGACATGTGTGAATTT+TGG - Chr5:27412921-27412940 MsG0580025934.01.T01:intron 40.0%
!!! TGTTTTGTTTGCATTGCCTG+AGG - Chr5:27413166-27413185 MsG0580025934.01.T01:intron 40.0%
AAGAACTTAGCACTACTGGG+AGG - Chr5:27414128-27414147 MsG0580025934.01.T01:intron 45.0%
ATAGTGATTCTGGCCCTTTG+AGG - Chr5:27413338-27413357 MsG0580025934.01.T01:intron 45.0%
CACCTCTCTTCAACTCATGT+TGG - Chr5:27411175-27411194 MsG0580025934.01.T01:intron 45.0%
CCTGCTACTATTCAACACTG+CGG - Chr5:27412845-27412864 MsG0580025934.01.T01:intron 45.0%
CCTGTTACCATCCCTCTAAA+AGG - Chr5:27414212-27414231 MsG0580025934.01.T01:intron 45.0%
CTGGGAACTTGCAACATCAT+TGG - Chr5:27412679-27412698 MsG0580025934.01.T01:intron 45.0%
TATGCTCCTACCATCTCAGA+AGG + Chr5:27412490-27412509 None:intergenic 45.0%
TTGCTTCTAAACTGCCGTTG+TGG - Chr5:27410928-27410947 MsG0580025934.01.T01:three_prime_UTR 45.0%
! ATGGGAATTGGTGGATCAAC+TGG + Chr5:27413418-27413437 None:intergenic 45.0%
! CGCAGTGTTGAATAGTAGCA+GGG + Chr5:27412847-27412866 None:intergenic 45.0%
! CTGTTCTTACCACTGGGTTT+TGG - Chr5:27412434-27412453 MsG0580025934.01.T01:intron 45.0%
! GAATTGGTGGATCAACTGGT+TGG + Chr5:27413414-27413433 None:intergenic 45.0%
! TCCCAACATGAGTTGAAGAG+AGG + Chr5:27411180-27411199 None:intergenic 45.0%
!! CCTTTTAGAGGGATGGTAAC+AGG + Chr5:27414215-27414234 None:intergenic 45.0%
!! GCCCTTTGAGGTTACATTCT+TGG - Chr5:27413350-27413369 MsG0580025934.01.T01:intron 45.0%
!! AAAAAGATAATAAAATTAAA+TGG + Chr5:27411285-27411304 None:intergenic 5.0%
!! ATAAAATTAAATTAAATGAA+AGG + Chr5:27411248-27411267 None:intergenic 5.0%
!! ATATTAAAAAATAAAAGAAA+AGG + Chr5:27411204-27411223 None:intergenic 5.0%
!! CAAATATTTAAATATTAATT+AGG - Chr5:27414020-27414039 MsG0580025934.01.T01:intron 5.0%
!! TATATATAATGATAATTATT+TGG + Chr5:27410803-27410822 None:intergenic 5.0%
!!! TATATAAGTATAATTTTTTT+AGG + Chr5:27411604-27411623 None:intergenic 5.0%
AAGGCTCATGAGTCATCCAG+AGG - Chr5:27413730-27413749 MsG0580025934.01.T01:intron 50.0%
CAACTGGTTGGACATTCAGC+TGG + Chr5:27413402-27413421 None:intergenic 50.0%
CAGGAAGGGAATCACCTGAT+AGG + Chr5:27412743-27412762 None:intergenic 50.0%
CTCATGAGTCATCCAGAGGA+TGG - Chr5:27413734-27413753 MsG0580025934.01.T01:intron 50.0%
TATGGAGCAGAACCATCCTC+TGG + Chr5:27413749-27413768 None:intergenic 50.0%
! CCGCAGTGTTGAATAGTAGC+AGG + Chr5:27412848-27412867 None:intergenic 50.0%
! GGAAAAAATGGAGGTGCGTC+TGG - Chr5:27413632-27413651 MsG0580025934.01.T01:intron 50.0%
! TCTGAACCTGCCTTCTGAGA+TGG - Chr5:27412477-27412496 MsG0580025934.01.T01:CDS 50.0%
! TGCCCTTTGAAGGGGCAATT+TGG - Chr5:27410737-27410756 MsG0580025934.01.T01:three_prime_UTR 50.0%
!! AACCTGCCTTCTGAGATGGT+AGG - Chr5:27412481-27412500 MsG0580025934.01.T01:CDS 50.0%
!! GTCAAGGCCTTTTAGAGGGA+TGG + Chr5:27414222-27414241 None:intergenic 50.0%
CTCCTACCATCTCAGAAGGC+AGG + Chr5:27412486-27412505 None:intergenic 55.0%
GGTGGCACTCTCCAAGGTTA+AGG + Chr5:27410776-27410795 None:intergenic 55.0%
GTAGCTTGGCCAAAACCCAG+TGG + Chr5:27412446-27412465 None:intergenic 55.0%
GGATGAGGTGGCACTCTCCA+AGG + Chr5:27410782-27410801 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 27410722 27414493 27410722 ID=MsG0580025934.01;Name=MsG0580025934.01
Chr5 mRNA 27410722 27414493 27410722 ID=MsG0580025934.01.T01;Parent=MsG0580025934.01;Name=MsG0580025934.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0.2|0.2|1|1|5|240|225
Chr5 exon 27414457 27414493 27414457 ID=MsG0580025934.01.T01:exon:29205;Parent=MsG0580025934.01.T01
Chr5 exon 27412717 27412811 27412717 ID=MsG0580025934.01.T01:exon:29206;Parent=MsG0580025934.01.T01
Chr5 exon 27412468 27412611 27412468 ID=MsG0580025934.01.T01:exon:29207;Parent=MsG0580025934.01.T01
Chr5 exon 27411759 27411968 27411759 ID=MsG0580025934.01.T01:exon:29208;Parent=MsG0580025934.01.T01
Chr5 exon 27410722 27411153 27410722 ID=MsG0580025934.01.T01:exon:29209;Parent=MsG0580025934.01.T01
Chr5 CDS 27414457 27414493 27414457 ID=MsG0580025934.01.T01:cds;Parent=MsG0580025934.01.T01
Chr5 CDS 27412717 27412811 27412717 ID=MsG0580025934.01.T01:cds;Parent=MsG0580025934.01.T01
Chr5 CDS 27412468 27412611 27412468 ID=MsG0580025934.01.T01:cds;Parent=MsG0580025934.01.T01
Chr5 CDS 27411759 27411968 27411759 ID=MsG0580025934.01.T01:cds;Parent=MsG0580025934.01.T01
Chr5 CDS 27410962 27411153 27410962 ID=MsG0580025934.01.T01:cds;Parent=MsG0580025934.01.T01
Chr5 three_prime_UTR 27410722 27410961 27410722 ID=MsG0580025934.01.T01:three_prime_utr;Parent=MsG0580025934.01.T01
Gene Sequence

>MsG0580025934.01.T01

ATGAATTTTCAATTTTGCCCTTTGAAGGGGCAATTTGATGTACATCCTGGAATTGACTTGACAGTTACTGTTCTTACCACTGGGTTTTGGCCAAGCTACAAGTCTTTTGATCTGAACCTGCCTTCTGAGATGGTCAAGTGTGTCGAAGTTTTTAAGGGCTTTTATGAAACAGAGACAAAACATAGAAAACTGACATTGATTTACTCACTGGGAACTTGCAACATCATTGGCAAGTTTGAGCCAAAAACCATCGAGTTAATTGTCTCAACCTATCAGGTCAAATTTCTTAAAGCTTGTGGTACTTTAGCAGGAACACGTGCTGAAATACGCAAAACATCGGCGAAACTGAAAGTGTCCCCTATTTGCGATAGTGATTCTGGCCCTTTGAGGTTACATTCTTGGCTTCCCAATACATCTGTTGAGAAACTCCAGCTGAATGTCCAACCAGTTGATCCACCAATTCCCATTAACAATGAACTTACTATGGAATCAGTGAATAACTCATTAGTAAACCTGAAGACATATCCATATGTTGATAGAGAAATAAGGAACAAGAACTTAGCACTACTGGGAGGTTACTATGATTTTGTGAATGGAGAATTCAAGTTGTGGAAGTATAAGACTCATGTCACTAATCCTGTTACCATCCCTCTAAAAGGCCTTGACATAACTATCTAA
Protein sequence

>MsG0580025934.01.T01

MNFQFCPLKGQFDVHPGIDLTVTVLTTGFWPSYKSFDLNLPSEMVKCVEVFKGFYETETKHRKLTLIYSLGTCNIIGKFEPKTIELIVSTYQVKFLKACGTLAGTRAEIRKTSAKLKVSPICDSDSGPLRLHSWLPNTSVEKLQLNVQPVDPPIPINNELTMESVNNSLVNLKTYPYVDREIRNKNLALLGGYYDFVNGEFKLWKYKTHVTNPVTIPLKGLDITI*