Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026613.01.T01 | AES98750.2 | 83.051 | 118 | 16 | 1 | 1 | 114 | 1 | 118 | 1.81E-60 | 194 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026613.01.T01 | G7KE99 | 83.051 | 118 | 16 | 1 | 1 | 114 | 1 | 118 | 8.63e-61 | 194 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004275.01 | MsG0580026613.01 | 0.815740 | 7.919154e-52 | 5.505619e-49 |
| MsG0180006134.01 | MsG0580026613.01 | 0.802752 | 4.832199e-49 | 2.386156e-46 |
| MsG0380011786.01 | MsG0580026613.01 | 0.801953 | 7.059327e-49 | 3.415463e-46 |
| MsG0380012215.01 | MsG0580026613.01 | 0.800175 | 1.629920e-48 | 7.538679e-46 |
| MsG0380013896.01 | MsG0580026613.01 | 0.804895 | 1.734528e-49 | 9.050277e-47 |
| MsG0480018664.01 | MsG0580026613.01 | 0.823111 | 1.650959e-53 | 1.408562e-50 |
| MsG0480021587.01 | MsG0580026613.01 | 0.811795 | 5.853840e-51 | 3.657893e-48 |
| MsG0580025934.01 | MsG0580026613.01 | 0.800084 | 1.701305e-48 | 7.850978e-46 |
| MsG0380016274.01 | MsG0580026613.01 | 0.819790 | 9.651230e-53 | 7.500668e-50 |
| MsG0480018121.01 | MsG0580026613.01 | 0.834263 | 3.317064e-56 | 3.915876e-53 |
| MsG0580026613.01 | MsG0580028169.01 | 0.807087 | 5.999009e-50 | 3.310887e-47 |
| MsG0580026613.01 | MsG0580028170.01 | 0.800018 | 1.754778e-48 | 8.083956e-46 |
| MsG0580026613.01 | MsG0580028794.01 | 0.803686 | 3.096539e-49 | 1.566024e-46 |
| MsG0580026613.01 | MsG0880044641.01 | 0.817276 | 3.585896e-52 | 2.599756e-49 |
| MsG0280010117.01 | MsG0580026613.01 | 0.804982 | 1.663439e-49 | 8.697981e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026613.01.T01 | MTR_5g072370 | 83.051 | 118 | 16 | 1 | 1 | 114 | 1 | 118 | 2.19e-64 | 194 |
| MsG0580026613.01.T01 | MTR_5g044895 | 82.203 | 118 | 17 | 1 | 1 | 114 | 1 | 118 | 3.93e-63 | 191 |
| MsG0580026613.01.T01 | MTR_6g043270 | 80.508 | 118 | 19 | 1 | 1 | 114 | 1 | 118 | 4.94e-61 | 186 |
| MsG0580026613.01.T01 | MTR_3g060830 | 79.661 | 118 | 20 | 1 | 1 | 114 | 1 | 118 | 5.69e-61 | 186 |
| MsG0580026613.01.T01 | MTR_3g028620 | 78.814 | 118 | 21 | 1 | 1 | 114 | 1 | 118 | 1.99e-59 | 182 |
| MsG0580026613.01.T01 | MTR_8g470030 | 78.814 | 118 | 21 | 1 | 1 | 114 | 1 | 118 | 2.39e-59 | 181 |
| MsG0580026613.01.T01 | MTR_4g045603 | 77.966 | 118 | 22 | 1 | 1 | 114 | 1 | 118 | 3.64e-58 | 178 |
| MsG0580026613.01.T01 | MTR_5g067630 | 75.424 | 118 | 25 | 1 | 1 | 114 | 1 | 118 | 4.59e-57 | 176 |
| MsG0580026613.01.T01 | MTR_3g033840 | 80.734 | 109 | 17 | 1 | 10 | 114 | 2 | 110 | 1.47e-56 | 173 |
| MsG0580026613.01.T01 | MTR_5g009790 | 76.271 | 118 | 24 | 1 | 1 | 114 | 1 | 118 | 1.54e-55 | 176 |
| MsG0580026613.01.T01 | MTR_7g061620 | 75.424 | 118 | 25 | 1 | 1 | 114 | 1 | 118 | 1.84e-55 | 171 |
| MsG0580026613.01.T01 | MTR_0005s0220 | 72.881 | 118 | 28 | 1 | 1 | 114 | 1 | 118 | 4.47e-55 | 171 |
| MsG0580026613.01.T01 | MTR_2g058650 | 73.729 | 118 | 27 | 1 | 1 | 114 | 1 | 118 | 6.20e-55 | 170 |
| MsG0580026613.01.T01 | MTR_5g063995 | 73.729 | 118 | 27 | 1 | 1 | 114 | 1 | 118 | 1.25e-54 | 169 |
| MsG0580026613.01.T01 | MTR_7g061360 | 74.576 | 118 | 26 | 1 | 1 | 114 | 1 | 118 | 6.85e-54 | 167 |
| MsG0580026613.01.T01 | MTR_2g083590 | 61.240 | 129 | 46 | 1 | 1 | 125 | 5 | 133 | 6.88e-50 | 157 |
| MsG0580026613.01.T01 | MTR_8g466300 | 80.000 | 100 | 16 | 1 | 19 | 114 | 12 | 111 | 6.92e-50 | 157 |
| MsG0580026613.01.T01 | MTR_5g053480 | 62.016 | 129 | 45 | 1 | 1 | 125 | 5 | 133 | 8.75e-50 | 157 |
| MsG0580026613.01.T01 | MTR_1g036730 | 69.492 | 118 | 32 | 1 | 1 | 114 | 5 | 122 | 1.18e-49 | 157 |
| MsG0580026613.01.T01 | MTR_0083s0070 | 81.443 | 97 | 14 | 1 | 22 | 114 | 1 | 97 | 5.23e-49 | 154 |
| MsG0580026613.01.T01 | MTR_4g061900 | 64.407 | 118 | 38 | 1 | 1 | 114 | 23 | 140 | 1.90e-46 | 147 |
| MsG0580026613.01.T01 | MTR_0214s0030 | 77.320 | 97 | 18 | 1 | 22 | 114 | 1 | 97 | 5.61e-45 | 144 |
| MsG0580026613.01.T01 | MTR_3g034000 | 72.000 | 100 | 24 | 1 | 1 | 96 | 1 | 100 | 5.96e-45 | 142 |
| MsG0580026613.01.T01 | MTR_0360s0010 | 76.000 | 100 | 17 | 2 | 22 | 114 | 1 | 100 | 7.30e-45 | 144 |
| MsG0580026613.01.T01 | MTR_6g036940 | 63.248 | 117 | 39 | 1 | 1 | 113 | 5 | 121 | 1.50e-44 | 145 |
| MsG0580026613.01.T01 | MTR_2g061240 | 84.615 | 78 | 12 | 0 | 37 | 114 | 3 | 80 | 1.96e-43 | 140 |
| MsG0580026613.01.T01 | MTR_1g053405 | 65.179 | 112 | 31 | 2 | 1 | 104 | 24 | 135 | 1.94e-42 | 137 |
| MsG0580026613.01.T01 | MTR_5g048800 | 73.196 | 97 | 22 | 1 | 22 | 114 | 1 | 97 | 1.98e-42 | 138 |
| MsG0580026613.01.T01 | MTR_4g069290 | 61.475 | 122 | 39 | 2 | 1 | 114 | 1 | 122 | 8.54e-42 | 137 |
| MsG0580026613.01.T01 | MTR_8g479440 | 87.500 | 72 | 9 | 0 | 43 | 114 | 38 | 109 | 7.75e-40 | 130 |
| MsG0580026613.01.T01 | MTR_4g054700 | 81.333 | 75 | 14 | 0 | 40 | 114 | 11 | 85 | 3.68e-37 | 124 |
| MsG0580026613.01.T01 | MTR_5g080380 | 69.565 | 92 | 24 | 1 | 27 | 114 | 1 | 92 | 2.08e-36 | 122 |
| MsG0580026613.01.T01 | MTR_4g022300 | 60.377 | 106 | 38 | 1 | 10 | 111 | 2 | 107 | 2.16e-36 | 120 |
| MsG0580026613.01.T01 | MTR_6g466390 | 84.615 | 65 | 10 | 0 | 50 | 114 | 23 | 87 | 1.65e-34 | 117 |
| MsG0580026613.01.T01 | MTR_6g042710 | 68.478 | 92 | 25 | 1 | 27 | 114 | 1 | 92 | 1.81e-34 | 117 |
| MsG0580026613.01.T01 | MTR_7g051090 | 68.478 | 92 | 25 | 1 | 27 | 114 | 1 | 92 | 2.48e-34 | 117 |
| MsG0580026613.01.T01 | MTR_8g075640 | 68.182 | 88 | 24 | 1 | 1 | 84 | 1 | 88 | 3.02e-34 | 114 |
| MsG0580026613.01.T01 | MTR_7g055890 | 53.279 | 122 | 49 | 2 | 1 | 114 | 1 | 122 | 3.75e-33 | 115 |
| MsG0580026613.01.T01 | MTR_5g055710 | 86.441 | 59 | 8 | 0 | 56 | 114 | 10 | 68 | 1.94e-31 | 109 |
| MsG0580026613.01.T01 | MTR_7g056780 | 65.823 | 79 | 26 | 1 | 48 | 125 | 17 | 95 | 1.00e-28 | 102 |
| MsG0580026613.01.T01 | MTR_0520s0020 | 85.965 | 57 | 8 | 0 | 58 | 114 | 1 | 57 | 1.08e-28 | 102 |
| MsG0580026613.01.T01 | MTR_4g026610 | 53.333 | 60 | 28 | 0 | 55 | 114 | 1 | 60 | 2.45e-15 | 66.6 |
| MsG0580026613.01.T01 | MTR_6g044850 | 71.429 | 42 | 12 | 0 | 73 | 114 | 1 | 42 | 3.67e-15 | 67.0 |
| MsG0580026613.01.T01 | MTR_5g084150 | 56.522 | 46 | 20 | 0 | 69 | 114 | 64 | 109 | 1.71e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATATCTACACTCCTTACTT+TGG | 0.387479 | 5:+40697020 | None:intergenic |
| ACAAAGGAAGTTGGTTAAGA+TGG | 0.449593 | 5:-40697077 | MsG0580026613.01.T01:CDS |
| CTCGAGTTGTGGTCATTATG+AGG | 0.481821 | 5:-40697263 | MsG0580026613.01.T01:CDS |
| TTCCCTTCATCATCACTACC+TGG | 0.483163 | 5:+40697232 | None:intergenic |
| CTGCAATTTAGCAATCTCTT+CGG | 0.485224 | 5:+40697336 | None:intergenic |
| GTTGGAAGAAATCCAAAGTA+AGG | 0.492461 | 5:-40697032 | MsG0580026613.01.T01:CDS |
| TGATGATGAAGGGAAGAAAG+AGG | 0.521727 | 5:-40697224 | MsG0580026613.01.T01:CDS |
| CAACCAGGTAGTGATGATGA+AGG | 0.544767 | 5:-40697235 | MsG0580026613.01.T01:CDS |
| CAATCTCTTCGGTGTCATAG+AGG | 0.561410 | 5:+40697347 | None:intergenic |
| CATTATGAGGCGAATCAACC+AGG | 0.564776 | 5:-40697250 | MsG0580026613.01.T01:CDS |
| ACATGAGTCACAAAGGAAGT+TGG | 0.578646 | 5:-40697086 | MsG0580026613.01.T01:CDS |
| TGAGTGTCACGAGGTTGTGT+TGG | 0.595153 | 5:-40697050 | MsG0580026613.01.T01:CDS |
| AACCAGGTAGTGATGATGAA+GGG | 0.609779 | 5:-40697234 | MsG0580026613.01.T01:CDS |
| GACAACAAAATCTCGAGTTG+TGG | 0.616717 | 5:-40697274 | MsG0580026613.01.T01:CDS |
| AGAAAGCACATGAGTCACAA+AGG | 0.657229 | 5:-40697093 | MsG0580026613.01.T01:CDS |
| GAGAAGTTTGATGTTCAGTG+TGG | 0.683538 | 5:-40697127 | MsG0580026613.01.T01:CDS |
| GATGGAGACTGAGTGTCACG+AGG | 0.720575 | 5:-40697059 | MsG0580026613.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATTTTAGATGAATTCT+TGG | - | Chr5:40697200-40697219 | MsG0580026613.01.T01:CDS | 15.0% |
| !!! | ATCTTTTAAGATCAAAATTT+TGG | - | Chr5:40697074-40697093 | MsG0580026613.01.T01:CDS | 15.0% |
| AATATCTACACTCCTTACTT+TGG | + | Chr5:40697353-40697372 | None:intergenic | 30.0% | |
| CTGCAATTTAGCAATCTCTT+CGG | + | Chr5:40697037-40697056 | None:intergenic | 35.0% | |
| GTTGGAAGAAATCCAAAGTA+AGG | - | Chr5:40697338-40697357 | MsG0580026613.01.T01:CDS | 35.0% | |
| ! | ACAAAGGAAGTTGGTTAAGA+TGG | - | Chr5:40697293-40697312 | MsG0580026613.01.T01:CDS | 35.0% |
| ACATGAGTCACAAAGGAAGT+TGG | - | Chr5:40697284-40697303 | MsG0580026613.01.T01:CDS | 40.0% | |
| GAGAAGTTTGATGTTCAGTG+TGG | - | Chr5:40697243-40697262 | MsG0580026613.01.T01:CDS | 40.0% | |
| ! | AACCAGGTAGTGATGATGAA+GGG | - | Chr5:40697136-40697155 | MsG0580026613.01.T01:CDS | 40.0% |
| ! | AGAAAGCACATGAGTCACAA+AGG | - | Chr5:40697277-40697296 | MsG0580026613.01.T01:CDS | 40.0% |
| ! | GACAACAAAATCTCGAGTTG+TGG | - | Chr5:40697096-40697115 | MsG0580026613.01.T01:CDS | 40.0% |
| ! | TGATGATGAAGGGAAGAAAG+AGG | - | Chr5:40697146-40697165 | MsG0580026613.01.T01:CDS | 40.0% |
| CATTATGAGGCGAATCAACC+AGG | - | Chr5:40697120-40697139 | MsG0580026613.01.T01:CDS | 45.0% | |
| TTCCCTTCATCATCACTACC+TGG | + | Chr5:40697141-40697160 | None:intergenic | 45.0% | |
| ! | CAACCAGGTAGTGATGATGA+AGG | - | Chr5:40697135-40697154 | MsG0580026613.01.T01:CDS | 45.0% |
| ! | CTCGAGTTGTGGTCATTATG+AGG | - | Chr5:40697107-40697126 | MsG0580026613.01.T01:CDS | 45.0% |
| !! | CAATCTCTTCGGTGTCATAG+AGG | + | Chr5:40697026-40697045 | None:intergenic | 45.0% |
| TGAGTGTCACGAGGTTGTGT+TGG | - | Chr5:40697320-40697339 | MsG0580026613.01.T01:CDS | 50.0% | |
| GATGGAGACTGAGTGTCACG+AGG | - | Chr5:40697311-40697330 | MsG0580026613.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 40697006 | 40697386 | 40697006 | ID=MsG0580026613.01;Name=MsG0580026613.01 |
| Chr5 | mRNA | 40697006 | 40697386 | 40697006 | ID=MsG0580026613.01.T01;Parent=MsG0580026613.01;Name=MsG0580026613.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|126 |
| Chr5 | exon | 40697006 | 40697386 | 40697006 | ID=MsG0580026613.01.T01:exon:37431;Parent=MsG0580026613.01.T01 |
| Chr5 | CDS | 40697006 | 40697386 | 40697006 | ID=MsG0580026613.01.T01:cds;Parent=MsG0580026613.01.T01 |
| Gene Sequence |
| Protein sequence |
