AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037678.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037678.01.T01 MTR_8g017040 80.556 72 14 0 50 121 1 72 1.06e-39 129
MsG0780037678.01.T01 MTR_3g436170 85.294 68 10 0 50 117 1 68 5.42e-39 126
MsG0780037678.01.T01 MTR_0013s0230 86.765 68 9 0 50 117 1 68 1.02e-36 132
MsG0780037678.01.T01 MTR_8g016975 85.294 68 10 0 50 117 1 68 4.88e-36 130
MsG0780037678.01.T01 MTR_4g005270 82.353 68 12 0 50 117 1 68 2.54e-35 128
MsG0780037678.01.T01 MTR_4g005190 80.882 68 13 0 50 117 1 68 3.20e-34 125
MsG0780037678.01.T01 MTR_4g005190 80.882 68 13 0 50 117 1 68 3.30e-34 124
MsG0780037678.01.T01 MTR_8g016960 80.882 68 13 0 50 117 1 68 1.49e-33 123
MsG0780037678.01.T01 MTR_5g008810 52.239 67 31 1 50 116 1 66 1.07e-17 77.8
MsG0780037678.01.T01 MTR_6g093180 52.174 69 31 1 50 118 1 67 1.09e-17 77.8
MsG0780037678.01.T01 MTR_7g062020 53.731 67 31 0 50 116 1 67 4.42e-17 76.3
MsG0780037678.01.T01 MTR_6g035010 51.471 68 31 1 50 117 1 66 8.04e-17 75.5
MsG0780037678.01.T01 MTR_6g088140 50.704 71 33 1 50 118 1 71 8.76e-17 75.5
MsG0780037678.01.T01 MTR_6g088140 50.704 71 33 1 50 118 1 71 8.99e-17 75.1
MsG0780037678.01.T01 MTR_6g034985 49.275 69 33 1 50 118 1 67 3.59e-16 73.6
MsG0780037678.01.T01 MTR_6g093100 50.725 69 32 1 50 116 1 69 3.48e-15 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037678.01.T01 AT1G78960 69.118 68 20 1 50 116 1 68 4.40e-27 104
MsG0780037678.01.T01 AT1G78960 69.118 68 20 1 50 116 1 68 4.40e-27 104
MsG0780037678.01.T01 AT1G78960 69.118 68 20 1 50 116 1 68 4.40e-27 104
MsG0780037678.01.T01 AT1G78960 69.118 68 20 1 50 116 1 68 4.47e-27 104
MsG0780037678.01.T01 AT1G66960 66.176 68 22 1 50 116 10 77 3.95e-26 102
MsG0780037678.01.T01 AT1G66960 66.176 68 22 1 50 116 1 68 4.10e-26 102
MsG0780037678.01.T01 AT1G66960 66.176 68 22 1 50 116 1 68 4.10e-26 102
MsG0780037678.01.T01 AT1G66960 66.176 68 22 1 50 116 1 68 4.10e-26 102
MsG0780037678.01.T01 AT1G78955 64.706 68 23 1 50 116 1 68 4.26e-25 99.0
MsG0780037678.01.T01 AT1G78970 66.176 68 22 1 50 116 1 68 4.73e-25 99.0
MsG0780037678.01.T01 AT1G78970 66.176 68 22 1 50 116 1 68 4.73e-25 99.0
MsG0780037678.01.T01 AT1G78970 66.176 68 22 1 50 116 1 68 4.73e-25 99.0
MsG0780037678.01.T01 AT1G78950 64.706 68 23 1 50 116 10 77 1.55e-24 97.4
MsG0780037678.01.T01 AT1G78950 64.706 68 23 1 50 116 1 68 1.67e-24 97.4
MsG0780037678.01.T01 AT5G36150 58.824 68 27 1 50 116 1 68 7.47e-22 89.7
MsG0780037678.01.T01 AT5G36150 58.824 68 27 1 50 116 1 68 7.47e-22 89.7
MsG0780037678.01.T01 AT4G15340 61.765 68 25 1 50 116 1 68 7.62e-22 89.7
MsG0780037678.01.T01 AT4G15340 61.765 68 25 1 50 116 1 68 8.63e-22 89.7
MsG0780037678.01.T01 AT4G15370 58.824 68 27 1 50 116 1 68 1.96e-20 85.9
MsG0780037678.01.T01 AT4G15370 58.824 68 27 1 50 116 1 68 1.98e-20 85.9
MsG0780037678.01.T01 AT5G42600 57.353 68 28 1 50 116 1 68 9.29e-20 84.0
MsG0780037678.01.T01 AT1G78500 57.353 68 28 1 50 116 1 68 9.85e-20 84.0
MsG0780037678.01.T01 AT1G78500 57.353 68 28 1 50 116 1 68 9.85e-20 84.0
MsG0780037678.01.T01 AT2G07050 58.209 67 27 1 50 116 1 66 1.07e-18 80.9
MsG0780037678.01.T01 AT5G48010 50.000 68 33 1 50 116 1 68 2.34e-17 77.0
MsG0780037678.01.T01 AT5G48010 50.000 68 33 1 50 116 1 68 2.39e-17 77.0
MsG0780037678.01.T01 AT3G29255 51.562 64 30 1 51 113 6 69 8.34e-16 72.4
MsG0780037678.01.T01 AT3G45130 50.746 67 29 1 50 116 1 63 8.84e-14 66.6
MsG0780037678.01.T01 AT3G45130 50.746 67 29 1 50 116 1 63 8.84e-14 66.6
MsG0780037678.01.T01 AT3G45130 50.746 67 29 1 50 116 1 63 8.84e-14 66.6
MsG0780037678.01.T01 AT3G45130 50.746 67 29 1 50 116 1 63 9.02e-14 66.6
MsG0780037678.01.T01 AT3G45130 50.746 67 29 1 50 116 1 63 9.02e-14 66.6

Find 26 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACGGCTCCTTCGTCGTATT+TGG 0.135041 7:+30842964 MsG0780037678.01.T01:CDS
TCGTTGCTTATTATATAAAT+AGG 0.241602 7:-30843821 None:intergenic
TCCTCCAAAGTACCTGCTTT+TGG 0.246773 7:-30843702 None:intergenic
ACACAATACTTCCTCGTATC+TGG 0.326531 7:+30843020 MsG0780037678.01.T01:CDS
ACTTTGTAGGAAGACAAATA+TGG 0.380103 7:+30843670 MsG0780037678.01.T01:CDS
TTCAGCACAAACAACTTTGT+AGG 0.391304 7:+30843657 MsG0780037678.01.T01:CDS
AGCTATGTGGTGACCTCCTT+TGG 0.416081 7:+30843781 MsG0780037678.01.T01:CDS
TTGTTTGTGCTGAATATGTA+TGG 0.427095 7:-30843648 None:intergenic
CACATAGCTTGACTTTAAAA+CGG 0.462109 7:-30843767 None:intergenic
TGATCCAAAAGCAGGTACTT+TGG 0.472154 7:+30843698 MsG0780037678.01.T01:CDS
GAAGTATTGTGTGCTTTGAT+TGG 0.473363 7:-30843009 None:intergenic
CTTTGTAGGAAGACAAATAT+GGG 0.516841 7:+30843671 MsG0780037678.01.T01:CDS
TAATAGAGAAAAGAAATATG+TGG 0.550501 7:+30843595 MsG0780037678.01.T01:CDS
ATGTGGTGATATTGCAAGCA+AGG 0.574667 7:-30843049 None:intergenic
TTGACAGTTCTAAATGGGAA+CGG 0.579118 7:+30842946 None:intergenic
TGGGAGTATGATCCAAAAGC+AGG 0.583052 7:+30843690 MsG0780037678.01.T01:CDS
TCCAAAAGCAGGTACTTTGG+AGG 0.594250 7:+30843701 MsG0780037678.01.T01:CDS
CAAGGAAAGAGCCAGATACG+AGG 0.629228 7:-30843031 None:intergenic
TAGAGAAAAGAAATATGTGG+AGG 0.642828 7:+30843598 MsG0780037678.01.T01:CDS
AACAACACTTTAAGAGCATG+TGG 0.642941 7:-30843066 None:intergenic
CAATAATCCAAATACGACGA+AGG 0.658702 7:-30842971 None:intergenic
AGGTTGAAGATAGCAGATGG+TGG 0.670662 7:+30843618 MsG0780037678.01.T01:CDS
AACGTACCTGAAAACGCCAA+AGG 0.675281 7:-30843797 None:intergenic
TGGAGGTTGAAGATAGCAGA+TGG 0.675749 7:+30843615 MsG0780037678.01.T01:CDS
TGTTTGTGCTGAATATGTAT+GGG 0.681487 7:-30843647 None:intergenic
GTACCTGAAAACGCCAAAGG+AGG 0.681958 7:-30843794 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTTATTATAAGAAATTAT+TGG + Chr7:30843381-30843400 MsG0780037678.01.T01:intron 10.0%
!! ATATTTAACATAATTACCTA+CGG + Chr7:30843472-30843491 MsG0780037678.01.T01:intron 15.0%
!!! AAATGAATTATTTTTTTGAG+TGG + Chr7:30843508-30843527 MsG0780037678.01.T01:intron 15.0%
!!! TTAGTTTTTAATTTGTGTTA+GGG + Chr7:30843568-30843587 MsG0780037678.01.T01:intron 15.0%
!! AACATTGTATAATATATGCA+TGG - Chr7:30843244-30843263 None:intergenic 20.0%
!! AATAAAGTGCGAATTAATTT+GGG - Chr7:30843369-30843388 None:intergenic 20.0%
!! AATTAAAATTGATGAAGAGT+TGG + Chr7:30843545-30843564 MsG0780037678.01.T01:intron 20.0%
!! TAATAAAGTGCGAATTAATT+TGG - Chr7:30843370-30843389 None:intergenic 20.0%
!! TAATAGAGAAAAGAAATATG+TGG + Chr7:30843595-30843614 MsG0780037678.01.T01:CDS 20.0%
!!! GTTAGTTTTTAATTTGTGTT+AGG + Chr7:30843567-30843586 MsG0780037678.01.T01:intron 20.0%
! AACATTATTCATGGTAATCT+AGG - Chr7:30843413-30843432 None:intergenic 25.0%
! GGACAGAATAATATCTTAAA+GGG - Chr7:30843348-30843367 None:intergenic 25.0%
! GTTAGATTGAACATTATTCA+TGG - Chr7:30843422-30843441 None:intergenic 25.0%
! TAATAAAGTGAGAATGATGT+AGG - Chr7:30843444-30843463 None:intergenic 25.0%
!!! TTATTTTTTTGAGTGGCAAT+TGG + Chr7:30843515-30843534 MsG0780037678.01.T01:intron 25.0%
ACTAATTAATCTAAGCTCGT+AGG + Chr7:30843267-30843286 MsG0780037678.01.T01:intron 30.0%
ACTTTGTAGGAAGACAAATA+TGG + Chr7:30843670-30843689 MsG0780037678.01.T01:CDS 30.0%
ATCTTAAAGGGATTGTTAGA+TGG - Chr7:30843336-30843355 None:intergenic 30.0%
ATTACGTGAACTGAATTCTT+AGG - Chr7:30843093-30843112 None:intergenic 30.0%
CTAATTAATCTAAGCTCGTA+GGG + Chr7:30843268-30843287 MsG0780037678.01.T01:intron 30.0%
CTTTGTAGGAAGACAAATAT+GGG + Chr7:30843671-30843690 MsG0780037678.01.T01:CDS 30.0%
GGGACAGAATAATATCTTAA+AGG - Chr7:30843349-30843368 None:intergenic 30.0%
GTTAGATGGAACATTATTTG+TGG - Chr7:30843322-30843341 None:intergenic 30.0%
TAGAGAAAAGAAATATGTGG+AGG + Chr7:30843598-30843617 MsG0780037678.01.T01:CDS 30.0%
TGTTTGTGCTGAATATGTAT+GGG - Chr7:30843650-30843669 None:intergenic 30.0%
TTATTCATGGTAATCTAGGT+TGG - Chr7:30843409-30843428 None:intergenic 30.0%
TTGTTTGTGCTGAATATGTA+TGG - Chr7:30843651-30843670 None:intergenic 30.0%
! CACATAGCTTGACTTTAAAA+CGG - Chr7:30843770-30843789 None:intergenic 30.0%
AACATAATTACCTACGGCTA+AGG + Chr7:30843478-30843497 MsG0780037678.01.T01:intron 35.0%
CAATAATCCAAATACGACGA+AGG - Chr7:30842974-30842993 None:intergenic 35.0%
TTCAGCACAAACAACTTTGT+AGG + Chr7:30843657-30843676 MsG0780037678.01.T01:CDS 35.0%
! AACAACACTTTAAGAGCATG+TGG - Chr7:30843069-30843088 None:intergenic 35.0%
! TTTGTATTTTCCTTAGCCGT+AGG - Chr7:30843491-30843510 None:intergenic 35.0%
!! GAAGTATTGTGTGCTTTGAT+TGG - Chr7:30843012-30843031 None:intergenic 35.0%
!!! CGTTTTAAAGTCAAGCTATG+TGG + Chr7:30843768-30843787 MsG0780037678.01.T01:CDS 35.0%
ACACAATACTTCCTCGTATC+TGG + Chr7:30843020-30843039 MsG0780037678.01.T01:CDS 40.0%
AGCTCGTAGGGACAATATTA+TGG + Chr7:30843280-30843299 MsG0780037678.01.T01:intron 40.0%
ATATTTACCGATGCGTGCTT+AGG - Chr7:30843140-30843159 None:intergenic 40.0%
ATGTGGTGATATTGCAAGCA+AGG - Chr7:30843052-30843071 None:intergenic 40.0%
TATTTACCGATGCGTGCTTA+GGG - Chr7:30843139-30843158 None:intergenic 40.0%
TGATCCAAAAGCAGGTACTT+TGG + Chr7:30843698-30843717 MsG0780037678.01.T01:CDS 40.0%
AACGTACCTGAAAACGCCAA+AGG - Chr7:30843800-30843819 None:intergenic 45.0%
AGGTTGAAGATAGCAGATGG+TGG + Chr7:30843618-30843637 MsG0780037678.01.T01:CDS 45.0%
ATCAATCCCTAAGCACGCAT+CGG + Chr7:30843130-30843149 MsG0780037678.01.T01:intron 45.0%
TCCAAAAGCAGGTACTTTGG+AGG + Chr7:30843701-30843720 MsG0780037678.01.T01:CDS 45.0%
TGGAGGTTGAAGATAGCAGA+TGG + Chr7:30843615-30843634 MsG0780037678.01.T01:CDS 45.0%
! TGGGAGTATGATCCAAAAGC+AGG + Chr7:30843690-30843709 MsG0780037678.01.T01:CDS 45.0%
!! TCCTCCAAAGTACCTGCTTT+TGG - Chr7:30843705-30843724 None:intergenic 45.0%
AACGGCTCCTTCGTCGTATT+TGG + Chr7:30842964-30842983 MsG0780037678.01.T01:CDS 50.0%
AGCTATGTGGTGACCTCCTT+TGG + Chr7:30843781-30843800 MsG0780037678.01.T01:CDS 50.0%
CAAGGAAAGAGCCAGATACG+AGG - Chr7:30843034-30843053 None:intergenic 50.0%
GTACCTGAAAACGCCAAAGG+AGG - Chr7:30843797-30843816 None:intergenic 50.0%
! TGACCTCCTTTGGCGTTTTC+AGG + Chr7:30843791-30843810 MsG0780037678.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 30842959 30843833 30842959 ID=MsG0780037678.01;Name=MsG0780037678.01
Chr7 mRNA 30842959 30843833 30842959 ID=MsG0780037678.01.T01;Parent=MsG0780037678.01;Name=MsG0780037678.01.T01;_AED=0.58;_eAED=0.65;_QI=0|0|0|1|0|0|2|0|122
Chr7 exon 30842959 30843082 30842959 ID=MsG0780037678.01.T01:exon:1379;Parent=MsG0780037678.01.T01
Chr7 exon 30843589 30843833 30843589 ID=MsG0780037678.01.T01:exon:1378;Parent=MsG0780037678.01.T01
Chr7 CDS 30842959 30843082 30842959 ID=MsG0780037678.01.T01:cds;Parent=MsG0780037678.01.T01
Chr7 CDS 30843589 30843833 30843589 ID=MsG0780037678.01.T01:cds;Parent=MsG0780037678.01.T01
Gene Sequence

>MsG0780037678.01.T01

ATGGGAACGGCTCCTTCGTCGTATTTGGATTATTGCTATATAATAAAGCACCAATCAAAGCACACAATACTTCCTCGTATCTGGCTCTTTCCTTGCTTGCAATATCACCACATGCTCTTAAAGTGGCTTATAATAGAGAAAAGAAATATGTGGAGGTTGAAGATAGCAGATGGTGGTAATGACCCATACATATTCAGCACAAACAACTTTGTAGGAAGACAAATATGGGAGTATGATCCAAAAGCAGGTACTTTGGAGGAGAGAGCACAAGTTGAAGAAGCTCGTCAGAATTTTTATAACAACCGTTTTAAAGTCAAGCTATGTGGTGACCTCCTTTGGCGTTTTCAGGTACGTTACCTATTTATATAA

Protein sequence

>MsG0780037678.01.T01

MGTAPSSYLDYCYIIKHQSKHTILPRIWLFPCLQYHHMLLKWLIIEKRNMWRLKIADGGNDPYIFSTNNFVGRQIWEYDPKAGTLEERAQVEEARQNFYNNRFKVKLCGDLLWRFQVRYLFI*