AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018121.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018121.01.T01 MTR_3g028620 79.082 196 37 1 1 192 1 196 6.70e-111 315
MsG0480018121.01.T01 MTR_8g470030 77.551 196 40 1 1 192 1 196 1.22e-106 304
MsG0480018121.01.T01 MTR_5g044895 78.061 196 39 1 1 192 1 196 8.05e-106 302
MsG0480018121.01.T01 MTR_5g072370 77.041 196 41 1 1 192 1 196 2.36e-105 301
MsG0480018121.01.T01 MTR_4g045603 76.531 196 42 1 1 192 1 196 6.75e-105 300
MsG0480018121.01.T01 MTR_3g060830 77.041 196 41 1 1 192 1 196 7.05e-105 300
MsG0480018121.01.T01 MTR_6g043270 76.020 196 43 1 1 192 1 196 1.30e-102 294
MsG0480018121.01.T01 MTR_0083s0070 81.143 175 29 1 22 192 1 175 4.20e-102 292
MsG0480018121.01.T01 MTR_5g067630 72.959 196 49 1 1 192 1 196 3.54e-99 285
MsG0480018121.01.T01 MTR_7g061620 73.469 196 48 1 1 192 1 196 4.61e-99 285
MsG0480018121.01.T01 MTR_2g058650 72.959 196 49 1 1 192 1 196 1.47e-98 283
MsG0480018121.01.T01 MTR_5g009790 73.333 195 48 1 1 191 1 195 6.22e-98 287
MsG0480018121.01.T01 MTR_0005s0220 70.918 196 53 1 1 192 1 196 1.83e-97 281
MsG0480018121.01.T01 MTR_7g061360 72.449 196 50 1 1 192 1 196 2.84e-97 280
MsG0480018121.01.T01 MTR_5g063995 71.939 196 51 1 1 192 1 196 3.10e-97 280
MsG0480018121.01.T01 MTR_0214s0030 77.714 175 35 1 22 192 1 175 6.46e-95 273
MsG0480018121.01.T01 MTR_8g466300 71.939 196 44 2 1 192 1 189 9.34e-95 274
MsG0480018121.01.T01 MTR_4g069290 67.500 200 57 2 1 192 1 200 1.19e-90 264
MsG0480018121.01.T01 MTR_2g061240 79.487 156 32 0 37 192 3 158 8.36e-90 260
MsG0480018121.01.T01 MTR_1g036730 65.816 196 63 1 1 192 5 200 1.38e-88 258
MsG0480018121.01.T01 MTR_5g048800 73.714 175 42 1 22 192 1 175 6.52e-88 256
MsG0480018121.01.T01 MTR_0360s0010 73.596 178 40 2 22 192 1 178 1.35e-87 255
MsG0480018121.01.T01 MTR_2g083590 63.265 196 67 2 1 192 5 199 2.88e-84 248
MsG0480018121.01.T01 MTR_4g054700 76.471 153 36 0 40 192 11 163 6.73e-83 243
MsG0480018121.01.T01 MTR_5g053480 63.265 196 67 2 1 192 5 199 1.71e-82 243
MsG0480018121.01.T01 MTR_3g033840 79.355 155 28 1 10 160 2 156 1.45e-80 236
MsG0480018121.01.T01 MTR_6g042710 65.294 170 55 1 27 192 1 170 2.50e-73 219
MsG0480018121.01.T01 MTR_5g055710 77.372 137 31 0 56 192 10 146 2.80e-72 215
MsG0480018121.01.T01 MTR_7g055890 56.000 200 80 2 1 192 1 200 4.95e-72 216
MsG0480018121.01.T01 MTR_0520s0020 75.556 135 33 0 58 192 1 135 8.00e-72 214
MsG0480018121.01.T01 MTR_7g051090 64.118 170 57 1 27 192 1 170 1.20e-71 214
MsG0480018121.01.T01 MTR_6g036940 54.286 210 78 3 1 192 5 214 6.87e-70 212
MsG0480018121.01.T01 MTR_7g056780 66.438 146 47 2 48 192 17 161 1.33e-63 194
MsG0480018121.01.T01 MTR_8g479440 82.203 118 21 0 43 160 38 155 3.83e-62 190
MsG0480018121.01.T01 MTR_5g080380 63.889 144 48 1 27 166 1 144 2.31e-58 180
MsG0480018121.01.T01 MTR_6g044850 63.333 120 44 0 73 192 1 120 5.94e-53 165
MsG0480018121.01.T01 MTR_3g117535 75.248 101 25 0 92 192 9 109 5.86e-51 160
MsG0480018121.01.T01 MTR_4g061900 60.294 136 50 1 1 132 23 158 1.05e-50 161
MsG0480018121.01.T01 MTR_3g034005 80.000 90 18 0 103 192 1 90 8.22e-49 154
MsG0480018121.01.T01 MTR_5g084150 58.065 124 52 0 69 192 64 187 2.76e-48 155
MsG0480018121.01.T01 MTR_6g466390 82.759 87 15 0 50 136 23 109 7.61e-47 151
MsG0480018121.01.T01 MTR_3g034000 71.000 100 25 1 1 96 1 100 6.24e-43 139
MsG0480018121.01.T01 MTR_1g053405 64.286 112 32 2 1 104 24 135 1.77e-41 137
MsG0480018121.01.T01 MTR_0134s0030 68.889 90 28 0 103 192 1 90 6.58e-38 126
MsG0480018121.01.T01 MTR_4g022300 58.182 110 42 1 10 115 2 111 2.49e-35 120
MsG0480018121.01.T01 MTR_8g075640 68.182 88 24 1 1 84 1 88 4.75e-34 116
MsG0480018121.01.T01 MTR_4g026610 45.238 84 34 1 55 126 1 84 8.64e-15 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 38 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTCTTCTTCCTCTTCTT+TGG 0.229861 4:+449825 None:intergenic
ATATCTACACTCCTTACTTT+TGG 0.254953 4:+450002 None:intergenic
ACTTCTTTGTTACAAATTCA+TGG 0.267623 4:-449856 MsG0480018121.01.T01:CDS
TTCTCAAAGTCTGAACCAAC+AGG 0.368570 4:-449978 MsG0480018121.01.T01:CDS
GTCTGAACCAACAGGGGTTA+AGG 0.381696 4:-449970 MsG0480018121.01.T01:CDS
GGAAATCACTTGTGTGCAAT+TGG 0.407497 4:-449949 MsG0480018121.01.T01:CDS
TGGATGAATGTCCTTCTCTA+AGG 0.407954 4:-449929 MsG0480018121.01.T01:CDS
TGCGCTCACAGTTAAACCTT+TGG 0.411587 4:+449890 None:intergenic
TCTTCTTCCTCTTCTTTGGT+AGG 0.418562 4:+449829 None:intergenic
TTCCCTTCACCATCACTACC+TGG 0.455355 4:+450215 None:intergenic
CTCGAGTTGTGGTCATTATG+AGG 0.481821 4:-450246 MsG0480018121.01.T01:CDS
GCAAAGGAAGTTGGTTAAGA+TGG 0.486781 4:-450060 MsG0480018121.01.T01:CDS
TGGAAGAAAATCCAAAAGTA+AGG 0.496893 4:-450013 MsG0480018121.01.T01:CDS
TGATGGTGAAGGGAAGAAAG+AGG 0.512770 4:-450207 MsG0480018121.01.T01:CDS
CTGCAACTTAGCAATCTCTT+CGG 0.512827 4:+450319 None:intergenic
AAGGTTTAACTGTGAGCGCA+TGG 0.518253 4:-449887 MsG0480018121.01.T01:CDS
ACATGAGTCGCAAAGGAAGT+TGG 0.528803 4:-450069 MsG0480018121.01.T01:CDS
GTGATTTCCTTAACCCCTGT+TGG 0.540189 4:+449963 None:intergenic
CGCTTCAACCACTGTTAGGG+TGG 0.541287 4:+449777 None:intergenic
TGGGATAGCTTAAATTCCAA+AGG 0.551642 4:-449906 MsG0480018121.01.T01:CDS
ATGAATGTCCTTCTCTAAGG+TGG 0.555194 4:-449926 MsG0480018121.01.T01:CDS
CAATCTCTTCGGTGTCATAG+AGG 0.561410 4:+450330 None:intergenic
ACCAAAGAAGAGGAAGAAGA+AGG 0.563516 4:-449826 MsG0480018121.01.T01:CDS
CATTATGAGGCGAATCAACC+AGG 0.564776 4:-450233 MsG0480018121.01.T01:CDS
AAGCTATCCCACCTTAGAGA+AGG 0.585524 4:+449918 None:intergenic
GCGAATCAACCAGGTAGTGA+TGG 0.586093 4:-450224 MsG0480018121.01.T01:CDS
TGAATGTCCTTCTCTAAGGT+GGG 0.589541 4:-449925 MsG0480018121.01.T01:CDS
TGAGTGTCACGAGGTTGTGT+TGG 0.595153 4:-450033 MsG0480018121.01.T01:CDS
CTCAAAGTCTGAACCAACAG+GGG 0.596555 4:-449976 MsG0480018121.01.T01:CDS
TGGAATTCCTACCAAAGAAG+AGG 0.597578 4:-449836 MsG0480018121.01.T01:CDS
AGGTTTAACTGTGAGCGCAT+GGG 0.597765 4:-449886 MsG0480018121.01.T01:CDS
CAACCAGGTAGTGATGGTGA+AGG 0.615922 4:-450218 MsG0480018121.01.T01:CDS
GACAACAAAATCTCGAGTTG+TGG 0.616717 4:-450257 MsG0480018121.01.T01:CDS
TCTCAAAGTCTGAACCAACA+GGG 0.619042 4:-449977 MsG0480018121.01.T01:CDS
AGAAAGCACATGAGTCGCAA+AGG 0.623323 4:-450076 MsG0480018121.01.T01:CDS
AACCAGGTAGTGATGGTGAA+GGG 0.627173 4:-450217 MsG0480018121.01.T01:CDS
GAGAAGTTTGATGTTCAGTG+TGG 0.683538 4:-450110 MsG0480018121.01.T01:CDS
GATGGAGACTGAGTGTCACG+AGG 0.720575 4:-450042 MsG0480018121.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATTTTAGATGAATTCT+TGG - Chr4:449985-450004 MsG0480018121.01.T01:CDS 15.0%
!!! ATCTTTTAAGATCAAAATTT+TGG - Chr4:449859-449878 MsG0480018121.01.T01:CDS 15.0%
! ACTTCTTTGTTACAAATTCA+TGG - Chr4:450282-450301 MsG0480018121.01.T01:CDS 25.0%
TGGAAGAAAATCCAAAAGTA+AGG - Chr4:450125-450144 MsG0480018121.01.T01:CDS 30.0%
! ATATCTACACTCCTTACTTT+TGG + Chr4:450139-450158 None:intergenic 30.0%
TGGGATAGCTTAAATTCCAA+AGG - Chr4:450232-450251 MsG0480018121.01.T01:CDS 35.0%
ACCAAAGAAGAGGAAGAAGA+AGG - Chr4:450312-450331 MsG0480018121.01.T01:CDS 40.0%
CTGCAACTTAGCAATCTCTT+CGG + Chr4:449822-449841 None:intergenic 40.0%
GAGAAGTTTGATGTTCAGTG+TGG - Chr4:450028-450047 MsG0480018121.01.T01:CDS 40.0%
GGAAATCACTTGTGTGCAAT+TGG - Chr4:450189-450208 MsG0480018121.01.T01:CDS 40.0%
TCCTTCTTCTTCCTCTTCTT+TGG + Chr4:450316-450335 None:intergenic 40.0%
TCTCAAAGTCTGAACCAACA+GGG - Chr4:450161-450180 MsG0480018121.01.T01:CDS 40.0%
TCTTCTTCCTCTTCTTTGGT+AGG + Chr4:450312-450331 None:intergenic 40.0%
TGGAATTCCTACCAAAGAAG+AGG - Chr4:450302-450321 MsG0480018121.01.T01:CDS 40.0%
TGGATGAATGTCCTTCTCTA+AGG - Chr4:450209-450228 MsG0480018121.01.T01:CDS 40.0%
TTCTCAAAGTCTGAACCAAC+AGG - Chr4:450160-450179 MsG0480018121.01.T01:CDS 40.0%
! ATGAATGTCCTTCTCTAAGG+TGG - Chr4:450212-450231 MsG0480018121.01.T01:CDS 40.0%
! GACAACAAAATCTCGAGTTG+TGG - Chr4:449881-449900 MsG0480018121.01.T01:CDS 40.0%
! GCAAAGGAAGTTGGTTAAGA+TGG - Chr4:450078-450097 MsG0480018121.01.T01:CDS 40.0%
!! TGAATGTCCTTCTCTAAGGT+GGG - Chr4:450213-450232 MsG0480018121.01.T01:CDS 40.0%
AAGCTATCCCACCTTAGAGA+AGG + Chr4:450223-450242 None:intergenic 45.0%
AAGGTTTAACTGTGAGCGCA+TGG - Chr4:450251-450270 MsG0480018121.01.T01:CDS 45.0%
ACATGAGTCGCAAAGGAAGT+TGG - Chr4:450069-450088 MsG0480018121.01.T01:CDS 45.0%
AGGTTTAACTGTGAGCGCAT+GGG - Chr4:450252-450271 MsG0480018121.01.T01:CDS 45.0%
CATTATGAGGCGAATCAACC+AGG - Chr4:449905-449924 MsG0480018121.01.T01:CDS 45.0%
CTCAAAGTCTGAACCAACAG+GGG - Chr4:450162-450181 MsG0480018121.01.T01:CDS 45.0%
GTGATTTCCTTAACCCCTGT+TGG + Chr4:450178-450197 None:intergenic 45.0%
TGCGCTCACAGTTAAACCTT+TGG + Chr4:450251-450270 None:intergenic 45.0%
! AACCAGGTAGTGATGGTGAA+GGG - Chr4:449921-449940 MsG0480018121.01.T01:CDS 45.0%
! AGAAAGCACATGAGTCGCAA+AGG - Chr4:450062-450081 MsG0480018121.01.T01:CDS 45.0%
! CTCGAGTTGTGGTCATTATG+AGG - Chr4:449892-449911 MsG0480018121.01.T01:CDS 45.0%
! TGATGGTGAAGGGAAGAAAG+AGG - Chr4:449931-449950 MsG0480018121.01.T01:CDS 45.0%
!! CAATCTCTTCGGTGTCATAG+AGG + Chr4:449811-449830 None:intergenic 45.0%
GCGAATCAACCAGGTAGTGA+TGG - Chr4:449914-449933 MsG0480018121.01.T01:CDS 50.0%
GTCTGAACCAACAGGGGTTA+AGG - Chr4:450168-450187 MsG0480018121.01.T01:CDS 50.0%
TGAGTGTCACGAGGTTGTGT+TGG - Chr4:450105-450124 MsG0480018121.01.T01:CDS 50.0%
TTCCCTTCACCATCACTACC+TGG + Chr4:449926-449945 None:intergenic 50.0%
! CAACCAGGTAGTGATGGTGA+AGG - Chr4:449920-449939 MsG0480018121.01.T01:CDS 50.0%
GATGGAGACTGAGTGTCACG+AGG - Chr4:450096-450115 MsG0480018121.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 449791 450369 449791 ID=MsG0480018121.01;Name=MsG0480018121.01
Chr4 mRNA 449791 450369 449791 ID=MsG0480018121.01.T01;Parent=MsG0480018121.01;Name=MsG0480018121.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|192
Chr4 exon 449791 450369 449791 ID=MsG0480018121.01.T01:exon:634;Parent=MsG0480018121.01.T01
Chr4 CDS 449791 450369 449791 ID=MsG0480018121.01.T01:cds;Parent=MsG0480018121.01.T01
Gene Sequence

>MsG0480018121.01.T01

ATGATGGACAGGGTACTCCTCTATGACACCGAAGAGATTGCTAAGTTGCAGTATGAAAAATATATGTTATCTTTTAAGATCAAAATTTTGGACAACAAAATCTCGAGTTGTGGTCATTATGAGGCGAATCAACCAGGTAGTGATGGTGAAGGGAAGAAAGAGGTGATCAACATCGATTACAATACTCAACTGCAAAATATTTTAGATGAATTCTTGGTGTCGAATCAAGTTTCATTTGAGAAGTTTGATGTTCAGTGTGGTGATCGTGTTGAGAAAGCACATGAGTCGCAAAGGAAGTTGGTTAAGATGGAGACTGAGTGTCACGAGGTTGTGTTGGAAGAAAATCCAAAAGTAAGGAGTGTAGATATTTTCTCAAAGTCTGAACCAACAGGGGTTAAGGAAATCACTTGTGTGCAATTGGATGAATGTCCTTCTCTAAGGTGGGATAGCTTAAATTCCAAAGGTTTAACTGTGAGCGCATGGGAGCATCTACTTCTTTGTTACAAATTCATGGAATTCCTACCAAAGAAGAGGAAGAAGAAGGATGATGTGTTTTTTCTAACATATTTGCCACCCTAA

Protein sequence

>MsG0480018121.01.T01

MMDRVLLYDTEEIAKLQYEKYMLSFKIKILDNKISSCGHYEANQPGSDGEGKKEVINIDYNTQLQNILDEFLVSNQVSFEKFDVQCGDRVEKAHESQRKLVKMETECHEVVLEENPKVRSVDIFSKSEPTGVKEITCVQLDECPSLRWDSLNSKGLTVSAWEHLLLCYKFMEFLPKKRKKKDDVFFLTYLPP*