Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028231.01.T01 | XP_039690679.1 | 96.386 | 83 | 3 | 0 | 1 | 83 | 1 | 83 | 1.81E-49 | 162 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028231.01.T01 | A0A393 | 80.723 | 83 | 16 | 0 | 1 | 83 | 1 | 83 | 3.71E-43 | 151 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028231.01.T01 | A0A0F6NL83 | 100.000 | 83 | 0 | 0 | 1 | 83 | 1 | 83 | 6.38e-49 | 175 |
| MsG0580028231.01.T01 | G7K1Z3 | 96.386 | 83 | 3 | 0 | 1 | 83 | 1 | 83 | 8.63e-50 | 162 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048689.01 | MsG0580028231.01 | 0.804629 | 1.970745e-49 | 1.021106e-46 |
| MsG0180002435.01 | MsG0580028231.01 | 0.801241 | 9.880412e-49 | 4.695265e-46 |
| MsG0380013930.01 | MsG0580028231.01 | 0.805286 | 1.436439e-49 | 7.569822e-47 |
| MsG0380013932.01 | MsG0580028231.01 | 0.814480 | 1.507645e-51 | 1.012815e-48 |
| MsG0380013939.01 | MsG0580028231.01 | 0.834710 | 2.560784e-56 | 3.063326e-53 |
| MsG0380013958.01 | MsG0580028231.01 | 0.803722 | 3.044447e-49 | 1.541075e-46 |
| MsG0380013965.01 | MsG0580028231.01 | 0.803873 | 2.832170e-49 | 1.439300e-46 |
| MsG0380013980.01 | MsG0580028231.01 | 0.816412 | 5.605819e-52 | 3.969243e-49 |
| MsG0380014022.01 | MsG0580028231.01 | 0.802529 | 5.372151e-49 | 2.637592e-46 |
| MsG0380014030.01 | MsG0580028231.01 | 0.826692 | 2.359281e-54 | 2.228632e-51 |
| MsG0380014036.01 | MsG0580028231.01 | 0.804485 | 2.111564e-49 | 1.090027e-46 |
| MsG0380014037.01 | MsG0580028231.01 | 0.802438 | 5.611020e-49 | 2.748572e-46 |
| MsG0380014038.01 | MsG0580028231.01 | 0.814494 | 1.497592e-51 | 1.006440e-48 |
| MsG0380014048.01 | MsG0580028231.01 | 0.840215 | 9.944564e-58 | 1.406844e-54 |
| MsG0380014056.01 | MsG0580028231.01 | 0.815223 | 1.032183e-51 | 7.074028e-49 |
| MsG0580028230.01 | MsG0580028231.01 | 0.809054 | 2.287331e-50 | 1.329238e-47 |
| MsG0580028231.01 | MsG0680033966.01 | 0.833100 | 6.474573e-56 | 7.381718e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028231.01.T01 | MTR_5g080950 | 96.386 | 83 | 3 | 0 | 1 | 83 | 1 | 83 | 2.19e-53 | 162 |
| MsG0580028231.01.T01 | MTR_4g006080 | 93.976 | 83 | 5 | 0 | 1 | 83 | 1 | 83 | 4.90e-47 | 159 |
| MsG0580028231.01.T01 | MTR_2126s0020 | 70.423 | 71 | 18 | 1 | 16 | 83 | 1 | 71 | 6.55e-31 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028231.01.T01 | ATCG01130 | 77.907 | 86 | 16 | 1 | 1 | 83 | 2 | 87 | 2.32e-42 | 146 |
| MsG0580028231.01.T01 | AT2G07739 | 76.744 | 86 | 17 | 1 | 1 | 83 | 1 | 86 | 3.60e-42 | 136 |
| MsG0580028231.01.T01 | ATCG01000 | 77.907 | 86 | 16 | 1 | 1 | 83 | 2 | 87 | 1.42e-40 | 136 |
| MsG0580028231.01.T01 | AT2G07565 | 71.831 | 71 | 17 | 1 | 16 | 83 | 1 | 71 | 5.05e-30 | 107 |
Find 25 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATAACCCGGGCTCGAATA+AGG | 0.285502 | 5:+77065624 | None:intergenic |
| CCTTATTCGAGCCCGGGTTA+TGG | 0.302023 | 5:-77065624 | MsG0580028231.01.T01:CDS |
| CTATTATGCGCCTTTGCATT+TGG | 0.302298 | 5:-77065528 | MsG0580028231.01.T01:CDS |
| AGCAACAACCGGGTTTATTA+CGG | 0.357699 | 5:-77065576 | MsG0580028231.01.T01:CDS |
| ATCTCTTCCTTATTCGAGCC+CGG | 0.378586 | 5:-77065631 | MsG0580028231.01.T01:CDS |
| ATTGTGATGGGACTCTATTA+TGG | 0.411319 | 5:-77065686 | MsG0580028231.01.T01:CDS |
| CGGTCCCTTCGTCCATAACC+CGG | 0.423231 | 5:+77065612 | None:intergenic |
| ACGGAAATAGCAGCAACAAC+CGG | 0.431789 | 5:-77065587 | MsG0580028231.01.T01:CDS |
| GCAACAACCGGGTTTATTAC+GGG | 0.432333 | 5:-77065575 | MsG0580028231.01.T01:CDS |
| GAAGAGATAAGAAGGCCCTA+TGG | 0.481996 | 5:+77065646 | None:intergenic |
| TGTTGCTGCTATTTCCGTCT+CGG | 0.484426 | 5:+77065592 | None:intergenic |
| TCTCTTCCTTATTCGAGCCC+GGG | 0.528342 | 5:-77065630 | MsG0580028231.01.T01:CDS |
| CGAATAAGGAAGAGATAAGA+AGG | 0.539148 | 5:+77065638 | None:intergenic |
| TTTCTGACTACATTCTCCAT+AGG | 0.543974 | 5:-77065662 | MsG0580028231.01.T01:CDS |
| ACTAAATTCAGCCATTGTGA+TGG | 0.544181 | 5:-77065699 | MsG0580028231.01.T01:CDS |
| TTCTGACTACATTCTCCATA+GGG | 0.544654 | 5:-77065661 | MsG0580028231.01.T01:CDS |
| CTAAATTCAGCCATTGTGAT+GGG | 0.553794 | 5:-77065698 | MsG0580028231.01.T01:CDS |
| CGGAAATAGCAGCAACAACC+GGG | 0.555341 | 5:-77065586 | MsG0580028231.01.T01:CDS |
| GGTCCCTTCGTCCATAACCC+GGG | 0.583354 | 5:+77065613 | None:intergenic |
| TCTAATCAAGCCAAATGCAA+AGG | 0.631817 | 5:+77065518 | None:intergenic |
| CGAGCCCGGGTTATGGACGA+AGG | 0.635728 | 5:-77065617 | MsG0580028231.01.T01:CDS |
| AAGCTGTCCCGTAATAAACC+CGG | 0.644525 | 5:+77065568 | None:intergenic |
| TATGGACGAAGGGACCGAGA+CGG | 0.657462 | 5:-77065606 | MsG0580028231.01.T01:CDS |
| GAGCCCGGGTTATGGACGAA+GGG | 0.668086 | 5:-77065616 | MsG0580028231.01.T01:CDS |
| ATAATAGAGTCCCATCACAA+TGG | 0.704177 | 5:+77065688 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGGCTGAATTTAGTATTTTA+AGG | + | Chr5:77065570-77065589 | None:intergenic | 25.0% |
| ACTAAATTCAGCCATTGTGA+TGG | - | Chr5:77065576-77065595 | MsG0580028231.01.T01:CDS | 35.0% | |
| ATAATAGAGTCCCATCACAA+TGG | + | Chr5:77065590-77065609 | None:intergenic | 35.0% | |
| CGAATAAGGAAGAGATAAGA+AGG | + | Chr5:77065640-77065659 | None:intergenic | 35.0% | |
| CTAAATTCAGCCATTGTGAT+GGG | - | Chr5:77065577-77065596 | MsG0580028231.01.T01:CDS | 35.0% | |
| TTCTGACTACATTCTCCATA+GGG | - | Chr5:77065614-77065633 | MsG0580028231.01.T01:CDS | 35.0% | |
| TTTCTGACTACATTCTCCAT+AGG | - | Chr5:77065613-77065632 | MsG0580028231.01.T01:CDS | 35.0% | |
| ! | ATTGTGATGGGACTCTATTA+TGG | - | Chr5:77065589-77065608 | MsG0580028231.01.T01:CDS | 35.0% |
| AGCAACAACCGGGTTTATTA+CGG | - | Chr5:77065699-77065718 | MsG0580028231.01.T01:CDS | 40.0% | |
| CTATTATGCGCCTTTGCATT+TGG | - | Chr5:77065747-77065766 | MsG0580028231.01.T01:CDS | 40.0% | |
| AAGCTGTCCCGTAATAAACC+CGG | + | Chr5:77065710-77065729 | None:intergenic | 45.0% | |
| ACGGAAATAGCAGCAACAAC+CGG | - | Chr5:77065688-77065707 | MsG0580028231.01.T01:CDS | 45.0% | |
| ATCTCTTCCTTATTCGAGCC+CGG | - | Chr5:77065644-77065663 | MsG0580028231.01.T01:CDS | 45.0% | |
| GAAGAGATAAGAAGGCCCTA+TGG | + | Chr5:77065632-77065651 | None:intergenic | 45.0% | |
| GCAACAACCGGGTTTATTAC+GGG | - | Chr5:77065700-77065719 | MsG0580028231.01.T01:CDS | 45.0% | |
| !! | TGTTGCTGCTATTTCCGTCT+CGG | + | Chr5:77065686-77065705 | None:intergenic | 45.0% |
| CGGAAATAGCAGCAACAACC+GGG | - | Chr5:77065689-77065708 | MsG0580028231.01.T01:CDS | 50.0% | |
| TCTCTTCCTTATTCGAGCCC+GGG | - | Chr5:77065645-77065664 | MsG0580028231.01.T01:CDS | 50.0% | |
| CCATAACCCGGGCTCGAATA+AGG | + | Chr5:77065654-77065673 | None:intergenic | 55.0% | |
| CCTTATTCGAGCCCGGGTTA+TGG | - | Chr5:77065651-77065670 | MsG0580028231.01.T01:CDS | 55.0% | |
| TATGGACGAAGGGACCGAGA+CGG | - | Chr5:77065669-77065688 | MsG0580028231.01.T01:CDS | 55.0% | |
| CGGTCCCTTCGTCCATAACC+CGG | + | Chr5:77065666-77065685 | None:intergenic | 60.0% | |
| GAGCCCGGGTTATGGACGAA+GGG | - | Chr5:77065659-77065678 | MsG0580028231.01.T01:CDS | 60.0% | |
| GGTCCCTTCGTCCATAACCC+GGG | + | Chr5:77065665-77065684 | None:intergenic | 60.0% | |
| CGAGCCCGGGTTATGGACGA+AGG | - | Chr5:77065658-77065677 | MsG0580028231.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 77065523 | 77065774 | 77065523 | ID=MsG0580028231.01;Name=MsG0580028231.01 |
| Chr5 | mRNA | 77065523 | 77065774 | 77065523 | ID=MsG0580028231.01.T01;Parent=MsG0580028231.01;Name=MsG0580028231.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|83 |
| Chr5 | exon | 77065523 | 77065774 | 77065523 | ID=MsG0580028231.01.T01:exon:24123;Parent=MsG0580028231.01.T01 |
| Chr5 | CDS | 77065523 | 77065774 | 77065523 | ID=MsG0580028231.01.T01:cds;Parent=MsG0580028231.01.T01 |
| Gene Sequence |
| Protein sequence |
