AlfalfaGEDB | MsG0580028230.01

M. sativa cultivar ZhongmuNo.1 / MsG0580028230.01

KEGG: -
GO: GO:0016021
PFAM: PF05758

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028230.01.T01	YP_009141729.1	89.209	139	15	0	1	139	166	304	4.70E-74	251

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028230.01.T01	Q09MB8	48.413	126	59	3	1	126	156	275	1.41E-29	115

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028230.01.T01	A0A0F6NLI4	89.209	139	15	0	1	139	166	304	2.25e-74	251

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180002226.01	MsG0580028230.01	0.816619	5.036378e-52	3.586455e-49
MsG0380013920.01	MsG0580028230.01	0.807667	4.519725e-50	2.532383e-47
MsG0380013939.01	MsG0580028230.01	0.808036	3.773323e-50	2.134712e-47
MsG0380013948.01	MsG0580028230.01	0.805547	1.266299e-49	6.718050e-47
MsG0380013949.01	MsG0580028230.01	0.833805	4.318468e-56	5.028550e-53
MsG0380013980.01	MsG0580028230.01	0.816075	6.665452e-52	4.676618e-49
MsG0380013995.01	MsG0580028230.01	0.810560	1.084751e-50	6.560005e-48
MsG0380014009.01	MsG0580028230.01	0.801732	7.837297e-49	3.770596e-46
MsG0380014011.01	MsG0580028230.01	0.804199	2.422635e-49	1.241443e-46
MsG0380014041.01	MsG0580028230.01	0.805466	1.317139e-49	6.973447e-47
MsG0380014048.01	MsG0580028230.01	0.820465	6.761794e-53	5.354493e-50
MsG0380014055.01	MsG0580028230.01	0.800769	1.233809e-48	5.793163e-46
MsG0580028230.01	MsG0580028231.01	0.809054	2.287331e-50	1.329238e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028230.01.T01	MTR_4g006080	87.500	128	12	2	1	124	163	290	1.03e-60	201
MsG0580028230.01.T01	MTR_1g110440	91.919	99	6	1	1	97	65	163	1.88e-59	181

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580028230.01.T01	ATCG01130	52.688	93	41	2	1	91	157	248	6.14e-26	102
MsG0580028230.01.T01	ATCG01000	52.688	93	41	2	1	91	157	248	6.36e-26	100

Find 15 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGATTGATATTATTAGTT+TGG	0.270523	5:-77065161	MsG0580028230.01.T01:CDS
GTTCTCGTTGCCCACTATTT+AGG	0.356468	5:-77065030	MsG0580028230.01.T01:CDS
TAAGTACATTCTATTACAAT+TGG	0.358341	5:-77065091	MsG0580028230.01.T01:CDS
TAAGAATAACAATTTATTGT+TGG	0.371195	5:+77065236	None:intergenic
ATTACAATTGGGAAATTATG+TGG	0.405012	5:-77065079	MsG0580028230.01.T01:CDS
AAGGAAAAGGATGAAATCAA+TGG	0.448984	5:-77064955	MsG0580028230.01.T01:CDS
AATCGATGTTGAAATAGATT+CGG	0.466965	5:-77064929	MsG0580028230.01.T01:CDS
AAGTACATTCTATTACAATT+GGG	0.486161	5:-77065090	MsG0580028230.01.T01:CDS
AGAATACGAAGAGAAGGAAA+AGG	0.512045	5:-77064968	MsG0580028230.01.T01:CDS
GCGGTGTTCTGCCTAAATAG+TGG	0.536523	5:+77065019	None:intergenic
CGGAAGAAAACAAAACAAAA+CGG	0.543163	5:-77064903	MsG0580028230.01.T01:CDS
CGGTGTTCTGCCTAAATAGT+GGG	0.549967	5:+77065020	None:intergenic
TTACAATTGGGAAATTATGT+GGG	0.569293	5:-77065078	MsG0580028230.01.T01:CDS
AATCTTAGAATACGAAGAGA+AGG	0.575849	5:-77064974	MsG0580028230.01.T01:CDS
TGTTGAAATAGATTCGGAAA+CGG	0.577018	5:-77064923	MsG0580028230.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTCATCAGTATAAAAATA+TGG	+	Chr5:77065116-77065135	None:intergenic	15.0%
!!	TAAGAATAACAATTTATTGT+TGG	+	Chr5:77064874-77064893	None:intergenic	15.0%
!!	AAGTACATTCTATTACAATT+GGG	-	Chr5:77065017-77065036	MsG0580028230.01.T01:CDS	20.0%
!!	TAAGTACATTCTATTACAAT+TGG	-	Chr5:77065016-77065035	MsG0580028230.01.T01:CDS	20.0%
!!	TTGGATTGATATTATTAGTT+TGG	-	Chr5:77064946-77064965	MsG0580028230.01.T01:CDS	20.0%
!!!	ATCTTTTTGATGAAATGTAT+TGG	-	Chr5:77064927-77064946	MsG0580028230.01.T01:CDS	20.0%
!	AATCGATGTTGAAATAGATT+CGG	-	Chr5:77065178-77065197	MsG0580028230.01.T01:CDS	25.0%
!	ATTACAATTGGGAAATTATG+TGG	-	Chr5:77065028-77065047	MsG0580028230.01.T01:CDS	25.0%
!	TCAGTATAAAAATATGGAAG+CGG	+	Chr5:77065110-77065129	None:intergenic	25.0%
!	TTACAATTGGGAAATTATGT+GGG	-	Chr5:77065029-77065048	MsG0580028230.01.T01:CDS	25.0%
!!	TTCTTAACAAGTAGTTTTCT+TGG	-	Chr5:77064888-77064907	MsG0580028230.01.T01:CDS	25.0%
	AAGGAAAAGGATGAAATCAA+TGG	-	Chr5:77065152-77065171	MsG0580028230.01.T01:CDS	30.0%
	AATCTTAGAATACGAAGAGA+AGG	-	Chr5:77065133-77065152	MsG0580028230.01.T01:CDS	30.0%
	CGGAAGAAAACAAAACAAAA+CGG	-	Chr5:77065204-77065223	MsG0580028230.01.T01:CDS	30.0%
	TGTTGAAATAGATTCGGAAA+CGG	-	Chr5:77065184-77065203	MsG0580028230.01.T01:CDS	30.0%
!	TAACAAGTAGTTTTCTTGGT+TGG	-	Chr5:77064892-77064911	MsG0580028230.01.T01:CDS	30.0%
!!	AGTTTTCTTGGTTGGTTAAT+TGG	-	Chr5:77064900-77064919	MsG0580028230.01.T01:CDS	30.0%
	AGAATACGAAGAGAAGGAAA+AGG	-	Chr5:77065139-77065158	MsG0580028230.01.T01:CDS	35.0%
	CGGTGTTCTGCCTAAATAGT+GGG	+	Chr5:77065090-77065109	None:intergenic	45.0%
	GTTCTCGTTGCCCACTATTT+AGG	-	Chr5:77065077-77065096	MsG0580028230.01.T01:CDS	45.0%
	GCGGTGTTCTGCCTAAATAG+TGG	+	Chr5:77065091-77065110	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	77064849	77065280	77064849	ID=MsG0580028230.01;Name=MsG0580028230.01
Chr5	mRNA	77064849	77065280	77064849	ID=MsG0580028230.01.T01;Parent=MsG0580028230.01;Name=MsG0580028230.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|143
Chr5	exon	77064849	77065280	77064849	ID=MsG0580028230.01.T01:exon:24122;Parent=MsG0580028230.01.T01
Chr5	CDS	77064849	77065280	77064849	ID=MsG0580028230.01.T01:cds;Parent=MsG0580028230.01.T01

Gene Sequence

>MsG0580028230.01.T01

ATGAACATTTATCTCTTTCGATCCAACAATAAATTGTTATTCTTAACAAGTAGTTTTCTTGGTTGGTTAATTGGTCATATCTTTTTGATGAAATGTATTGGATTGATATTATTAGTTTGGTCAAAGCAAAAAAATTCGATCAAATCTAAGTTAGCTATGCGATTTGATAAGTACATTCTATTACAATTGGGAAATTATGTGGGTCAAATATTTGTTGTTTTTTCATTTGTTCTCGTTGCCCACTATTTAGGCAGAACACCGCTTCCATATTTTTATACTGATGAAATCTTAGAATACGAAGAGAAGGAAAAGGATGAAATCAATGGAGAAATCGATGTTGAAATAGATTCGGAAACGGAAGAAAACAAAACAAAACGGCTTCATCGAATACGAAGAAAATCTTATTTCTTATCTTTTTCTGAAAAAAGATAA

Protein sequence

>MsG0580028230.01.T01

MNIYLFRSNNKLLFLTSSFLGWLIGHIFLMKCIGLILLVWSKQKNSIKSKLAMRFDKYILLQLGNYVGQIFVVFSFVLVAHYLGRTPLPYFYTDEILEYEEKEKDEINGEIDVEIDSETEENKTKRLHRIRRKSYFLSFSEKR*