AlfalfaGEDB | MsG0380013939.01

M. sativa cultivar ZhongmuNo.1 / MsG0380013939.01

KEGG: K03040
GO: GO:0003677 GO:0003899 GO:0006351
PFAM: PF03118

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013939.01.T01	RHN59741.1	99.2	125	1	0	1	125	170	294	2.55E-80	248
MsG0380013939.01.T02	RHN59741.1	98.913	92	1	0	1	92	203	294	6.31E-55	182

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013939.01.T01	Q8MC99	83.2	125	21	0	1	125	209	333	2.04E-67	209
MsG0380013939.01.T02	P13911	84.946	93	12	1	2	92	242	334	2.52E-47	156

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013939.01.T01	G7JH57	99.200	125	1	1	125	318	442	4.42e-80	251
MsG0380013939.01.T02	A0A396I4L8	98.913	92	1	1	92	203	294	3.02e-55	182
MsG0380013939.01.T02	G7JH57	98.913	92	1	1	92	351	442	1.58e-54	184

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048689.01	MsG0380013939.01	0.846716	1.823395e-59	3.167036e-56
MsG0080049016.01	MsG0380013939.01	0.816975	4.191166e-52	3.013962e-49
MsG0080049078.01	MsG0380013939.01	0.846893	1.631529e-59	2.849424e-56
MsG0080049079.01	MsG0380013939.01	0.840765	7.140314e-58	1.027458e-54
MsG0080049080.01	MsG0380013939.01	0.823584	1.280563e-53	1.107175e-50
MsG0180000502.01	MsG0380013939.01	0.829527	4.896801e-55	5.024659e-52
MsG0180002226.01	MsG0380013939.01	0.807075	6.034618e-50	3.329472e-47
MsG0180002435.01	MsG0380013939.01	0.801079	1.066329e-48	5.046726e-46
MsG0180003274.01	MsG0380013939.01	0.804401	2.199258e-49	1.132816e-46
MsG0180003933.01	MsG0380013939.01	0.804531	2.065543e-49	1.067520e-46
MsG0180004187.01	MsG0380013939.01	0.822820	1.930510e-53	1.633540e-50
MsG0180004302.01	MsG0380013939.01	0.819284	1.259256e-52	9.648375e-50
MsG0180004937.01	MsG0380013939.01	0.838156	3.400489e-57	4.514997e-54
MsG0180004987.01	MsG0380013939.01	0.817909	2.582538e-52	1.905114e-49
MsG0180005568.01	MsG0380013939.01	0.808147	3.572258e-50	2.027038e-47
MsG0180005879.01	MsG0380013939.01	0.811982	5.332105e-51	3.348562e-48
MsG0280010974.01	MsG0380013939.01	0.810015	1.422313e-50	8.479420e-48
MsG0280011270.01	MsG0380013939.01	0.803922	2.766135e-49	1.407542e-46
MsG0380012443.01	MsG0380013939.01	0.826435	2.717780e-54	2.548595e-51
MsG0380013889.01	MsG0380013939.01	0.849793	2.574203e-60	4.936583e-57
MsG0380013918.01	MsG0380013939.01	0.825111	5.599734e-54	5.056925e-51
MsG0380013919.01	MsG0380013939.01	0.847534	1.088057e-59	1.940173e-56
MsG0380013927.01	MsG0380013939.01	0.830765	2.442213e-55	2.597984e-52
MsG0380013930.01	MsG0380013939.01	0.834790	2.444584e-56	2.931421e-53
MsG0380013931.01	MsG0380013939.01	0.809498	1.837253e-50	1.080408e-47
MsG0380013932.01	MsG0380013939.01	0.840046	1.100772e-57	1.549291e-54
MsG0380013933.01	MsG0380013939.01	0.803851	2.862158e-49	1.453686e-46
MsG0380013935.01	MsG0380013939.01	0.808230	3.429451e-50	1.950251e-47
MsG0380013939.01	MsG0380013949.01	0.815712	8.031600e-52	5.579395e-49
MsG0380013939.01	MsG0380013955.01	0.854984	8.552263e-62	1.944880e-58
MsG0380013939.01	MsG0380013958.01	0.858343	8.792093e-63	2.236673e-59
MsG0380013939.01	MsG0380013963.01	0.845767	3.307724e-59	5.571850e-56
MsG0380013939.01	MsG0380013964.01	0.873960	9.806403e-68	4.336012e-64
MsG0380013939.01	MsG0380013965.01	0.849723	2.692163e-60	5.151157e-57
MsG0380013939.01	MsG0380013970.01	0.853790	1.892886e-61	4.138175e-58
MsG0380013939.01	MsG0380013978.01	0.843131	1.692247e-58	2.621825e-55
MsG0380013939.01	MsG0380013979.01	0.854236	1.407619e-61	3.123339e-58
MsG0380013939.01	MsG0380013980.01	0.868462	6.415758e-66	2.322648e-62
MsG0380013939.01	MsG0380013981.01	0.826684	2.370012e-54	2.238271e-51
MsG0380013939.01	MsG0380013983.01	0.835665	1.470052e-56	1.809873e-53
MsG0380013939.01	MsG0380013992.01	0.834665	2.628167e-56	3.139894e-53
MsG0380013939.01	MsG0380013995.01	0.813021	3.159686e-51	2.040166e-48
MsG0380013939.01	MsG0380013996.01	0.854658	1.062844e-61	2.391079e-58
MsG0380013939.01	MsG0380014004.01	0.804728	1.879154e-49	9.762420e-47
MsG0380013939.01	MsG0380014007.01	0.836405	9.538219e-57	1.200815e-53
MsG0380013939.01	MsG0380014009.01	0.808792	2.602025e-50	1.501562e-47
MsG0380013939.01	MsG0380014010.01	0.827484	1.525246e-54	1.474498e-51
MsG0380013939.01	MsG0380014012.01	0.833362	5.571813e-56	6.402261e-53
MsG0380013939.01	MsG0380014013.01	0.847855	8.881293e-60	1.599657e-56
MsG0380013939.01	MsG0380014022.01	0.870677	1.217900e-66	4.778092e-63
MsG0380013939.01	MsG0380014028.01	0.833034	6.724309e-56	7.651222e-53
MsG0380013939.01	MsG0380014030.01	0.848196	7.150146e-60	1.301757e-56
MsG0380013939.01	MsG0380014034.01	0.819156	1.346395e-52	1.027973e-49
MsG0380013939.01	MsG0380014035.01	0.839811	1.267572e-57	1.770685e-54
MsG0380013939.01	MsG0380014036.01	0.819831	9.445125e-53	7.348570e-50
MsG0380013939.01	MsG0380014037.01	0.859694	3.463823e-63	9.228511e-60
MsG0380013939.01	MsG0380014038.01	0.851041	1.148673e-60	2.295069e-57
MsG0380013939.01	MsG0380014041.01	0.806610	7.567207e-50	4.125229e-47
MsG0380013939.01	MsG0380014042.01	0.831260	1.846034e-55	1.992552e-52
MsG0380013939.01	MsG0380014048.01	0.873986	9.607466e-68	4.252476e-64
MsG0380013939.01	MsG0380014050.01	0.824870	6.386049e-54	5.727630e-51
MsG0380013939.01	MsG0380014053.01	0.839930	1.180170e-57	1.654798e-54
MsG0380013939.01	MsG0380014055.01	0.805791	1.125530e-49	6.009131e-47
MsG0380013939.01	MsG0380014056.01	0.838464	2.831212e-57	3.795215e-54
MsG0380013939.01	MsG0380015733.01	0.817539	3.128703e-52	2.284852e-49
MsG0380013939.01	MsG0380015898.01	0.810302	1.232959e-50	7.405250e-48
MsG0380013939.01	MsG0380016459.01	0.800491	1.405568e-48	6.553579e-46
MsG0380013939.01	MsG0380016723.01	0.830444	2.926225e-55	3.083435e-52
MsG0380013939.01	MsG0380016751.01	0.818572	1.827365e-52	1.372887e-49
MsG0380013939.01	MsG0480018089.01	0.826636	2.432971e-54	2.294591e-51
MsG0380013939.01	MsG0480018983.01	0.835416	1.700040e-56	2.077560e-53
MsG0380013939.01	MsG0480021420.01	0.832078	1.160377e-55	1.283284e-52
MsG0380013939.01	MsG0480022297.01	0.802418	5.662445e-49	2.772500e-46
MsG0380013939.01	MsG0480022804.01	0.806115	9.620408e-50	5.178947e-47
MsG0380013939.01	MsG0480023371.01	0.806175	9.344771e-50	5.038391e-47
MsG0380013939.01	MsG0480023505.01	0.813983	1.941224e-51	1.286528e-48
MsG0380013939.01	MsG0480023708.01	0.827634	1.404182e-54	1.363203e-51
MsG0380013939.01	MsG0480023881.01	0.809549	1.791440e-50	1.054923e-47
MsG0380013939.01	MsG0580026430.01	0.820122	8.103906e-53	6.357098e-50
MsG0380013939.01	MsG0580027254.01	0.813048	3.116676e-51	2.013831e-48
MsG0380013939.01	MsG0580028230.01	0.808036	3.773323e-50	2.134712e-47
MsG0380013939.01	MsG0580028231.01	0.834710	2.560784e-56	3.063326e-53
MsG0380013939.01	MsG0580028853.01	0.818279	2.129799e-52	1.587175e-49
MsG0380013939.01	MsG0580029673.01	0.800333	1.513733e-48	7.029871e-46
MsG0380013939.01	MsG0680032339.01	0.806693	7.268182e-50	3.970651e-47
MsG0380013939.01	MsG0680033966.01	0.837801	4.193890e-57	5.509553e-54
MsG0380013939.01	MsG0780037705.01	0.810255	1.262592e-50	7.574061e-48
MsG0380013939.01	MsG0780038140.01	0.803869	2.837618e-49	1.441929e-46
MsG0380013939.01	MsG0780039859.01	0.825092	5.660560e-54	5.109161e-51
MsG0380013939.01	MsG0780040315.01	0.832812	7.634295e-56	8.628263e-53
MsG0380013939.01	MsG0780040316.01	0.815171	1.059605e-51	7.251728e-49
MsG0380013939.01	MsG0780040317.01	0.830607	2.670016e-55	2.827055e-52
MsG0380013939.01	MsG0780041022.01	0.812751	3.622001e-51	2.321952e-48
MsG0380013939.01	MsG0780041024.01	0.816788	4.616704e-52	3.302801e-49
MsG0380013939.01	MsG0780041792.01	0.835660	1.474681e-56	1.815291e-53
MsG0380013939.01	MsG0880042006.01	0.801108	1.051696e-48	4.981176e-46
MsG0380013939.01	MsG0880042921.01	0.800265	1.562658e-48	7.244510e-46
MsG0380013939.01	MsG0880043541.01	0.853086	3.015025e-61	6.437692e-58
MsG0380013939.01	MsG0880044440.01	0.822418	2.394560e-53	2.003167e-50
MsG0380013939.01	MsG0880045261.01	0.831231	1.876827e-55	2.024137e-52
MsG0380013939.01	MsG0880045283.01	0.849447	3.215239e-60	6.096633e-57
MsG0380013939.01	MsG0880045412.01	0.827577	1.448706e-54	1.404133e-51
MsG0380013939.01	MsG0880046288.01	0.819901	9.102753e-53	7.096131e-50
MsG0380013939.01	MsG0880046456.01	0.830133	3.485897e-55	3.640489e-52
MsG0380013939.01	MsG0880046856.01	0.809948	1.470327e-50	8.749890e-48
MsG0380013939.01	MsG0880047251.01	0.804141	2.491356e-49	1.274780e-46
MsG0280007026.01	MsG0380013939.01	0.825524	4.471605e-54	4.085705e-51
MsG0280007041.01	MsG0380013939.01	0.847959	8.314456e-60	1.502502e-56
MsG0280007042.01	MsG0380013939.01	0.873505	1.395960e-67	6.073910e-64
MsG0280007600.01	MsG0380013939.01	0.810638	1.043321e-50	6.322658e-48
MsG0280008233.01	MsG0380013939.01	0.803484	3.409761e-49	1.715550e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013939.01.T01	MTR_4g051380	99.200	125	1	0	1	125	318	442	1.12e-83	251
MsG0380013939.01.T01	MTR_0002s0760	65.323	124	39	2	1	124	315	434	1.73e-48	160
MsG0380013939.01.T02	MTR_4g051380	98.913	92	1	0	1	92	351	442	4.00e-58	184
MsG0380013939.01.T02	MTR_0002s0760	58.889	90	33	2	1	90	348	433	3.73e-27	102

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013939.01.T01	ATCG00740	72.269	119	33	0	1	119	209	327	1.49e-54	173
MsG0380013939.01.T02	ATCG00740	70.930	86	25	0	1	86	242	327	3.91e-36	124

Find 13 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTATCATGAAAAGTAAAA+AGG	0.241741	3:+48467632	None:intergenic
GTCCAATATACATACATTAT+TGG	0.258629	3:-48467506	MsG0380013939.01.T01:CDS
TAAATAAATCAATCAAATTC+CGG	0.303178	3:+48467722	None:intergenic
TTCCAATAATGTATGTATAT+TGG	0.305613	3:+48467504	None:intergenic
TTTAAGACAATTATAGATCC+TGG	0.369970	3:+48467531	None:intergenic
GCTTCATAAAGTGCTTCTTT+AGG	0.371009	3:+48467748	None:intergenic
TCCTGGGAGACAATTCTAAT+TGG	0.386134	3:+48467548	None:intergenic
AAATAAATCAATCAAATTCC+GGG	0.398418	3:+48467723	None:intergenic
TTAAGACAATTATAGATCCT+GGG	0.489900	3:+48467532	None:intergenic
ACCAATTAGAATTGTCTCCC+AGG	0.525817	3:-48467549	MsG0380013939.01.T01:CDS
GATTGCTTAAGGATAAACTA+AGG	0.541266	3:-48467612	MsG0380013939.01.T01:CDS
AAGCACTTTATGAAGCCTCC+CGG	0.587455	3:-48467741	MsG0380013939.01.T01:CDS
TAAATCAATCAAATTCCGGG+AGG	0.721527	3:+48467726	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAATAAATCAATCAAATTC+CGG	+	Chr3:48467434-48467453	None:intergenic	15.0%
!!	AAATAAATCAATCAAATTCC+GGG	+	Chr3:48467433-48467452	None:intergenic	20.0%
!!	ATCTATCATGAAAAGTAAAA+AGG	+	Chr3:48467524-48467543	None:intergenic	20.0%
!!	TCTATCATGAAAAGTAAAAA+GGG	+	Chr3:48467523-48467542	None:intergenic	20.0%
!!	TTCCAATAATGTATGTATAT+TGG	+	Chr3:48467652-48467671	None:intergenic	20.0%
!!	TTCTAATTGGTCAATAAAAA+TGG	+	Chr3:48467595-48467614	None:intergenic	20.0%
!	CTATCATGAAAAGTAAAAAG+GGG	+	Chr3:48467522-48467541	None:intergenic	25.0%
!	GTCCAATATACATACATTAT+TGG	-	Chr3:48467647-48467666	MsG0380013939.01.T01:CDS	25.0%
!	TTAAGACAATTATAGATCCT+GGG	+	Chr3:48467624-48467643	None:intergenic	25.0%
!!	GAAGATGTTCAATTTTCATA+AGG	+	Chr3:48467698-48467717	None:intergenic	25.0%
!!	TTTAAGACAATTATAGATCC+TGG	+	Chr3:48467625-48467644	None:intergenic	25.0%
!!	TTTTCATGATAGATTGCTTA+AGG	-	Chr3:48467530-48467549	MsG0380013939.01.T01:CDS	25.0%
	GATTGCTTAAGGATAAACTA+AGG	-	Chr3:48467541-48467560	MsG0380013939.01.T01:CDS	30.0%
	TCTTCTTCTGCATGTAAAAA+AGG	+	Chr3:48467459-48467478	None:intergenic	30.0%
	TAAATCAATCAAATTCCGGG+AGG	+	Chr3:48467430-48467449	None:intergenic	35.0%
!	GCTTCATAAAGTGCTTCTTT+AGG	+	Chr3:48467408-48467427	None:intergenic	35.0%
	ACCAATTAGAATTGTCTCCC+AGG	-	Chr3:48467604-48467623	MsG0380013939.01.T01:CDS	40.0%
	TCCTGGGAGACAATTCTAAT+TGG	+	Chr3:48467608-48467627	None:intergenic	40.0%
	AAGCACTTTATGAAGCCTCC+CGG	-	Chr3:48467412-48467431	MsG0380013939.01.T01:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	48467399	48467776	48467399	ID=MsG0380013939.01;Name=MsG0380013939.01
Chr3	mRNA	48467399	48467776	48467399	ID=MsG0380013939.01.T01;Parent=MsG0380013939.01;Name=MsG0380013939.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|125
Chr3	exon	48467399	48467776	48467399	ID=MsG0380013939.01.T01:exon:9365;Parent=MsG0380013939.01.T01
Chr3	CDS	48467399	48467776	48467399	ID=MsG0380013939.01.T01:cds;Parent=MsG0380013939.01.T01
Chr3	mRNA	48467399	48467677	48467399	ID=MsG0380013939.01.T02;Parent=MsG0380013939.01;Name=MsG0380013939.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|92
Chr3	exon	48467399	48467677	48467399	ID=MsG0380013939.01.T02:exon:9366;Parent=MsG0380013939.01.T02
Chr3	CDS	48467399	48467677	48467399	ID=MsG0380013939.01.T02:cds;Parent=MsG0380013939.01.T02
Chr3	mRNA	48467399	48467476	48467399	ID=MsG0380013939.01.T03;Parent=MsG0380013939.01;Name=MsG0380013939.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|25
Chr3	exon	48467399	48467476	48467399	ID=MsG0380013939.01.T03:exon:9367;Parent=MsG0380013939.01.T03
Chr3	CDS	48467399	48467476	48467399	ID=MsG0380013939.01.T03:cds;Parent=MsG0380013939.01.T03

Gene Sequence

>MsG0380013939.01.T01

TTAACTCCTAAAGAAGCACTTTATGAAGCCTCCCGGAATTTGATTGATTTATTTATTCCTTTTTTACATGCAGAAGAAGAAAACTTAAATTTTGAAAATAATCAACACAAAGTTACTTTACCCCTTTTTACTTTTCATGATAGATTGCTTAAGGATAAACTAAGGAAAAATAAAAAAGAAATTGCATTGAAATCCATTTTTATTGACCAATTAGAATTGTCTCCCAGGATCTATAATTGTCTTAAAAAGTCCAATATACATACATTATTGGAACTTTTGAATAAGAGTCAAGAAGACCTTATGAAAATTGAACATCTTCGCGTAGAAGATGTCAAAAATATATTAAACATTTTACAAATCGAAAAGCATTTTGCATAA

>MsG0380013939.01.T02

AATCAACACAAAGTTACTTTACCCCTTTTTACTTTTCATGATAGATTGCTTAAGGATAAACTAAGGAAAAATAAAAAAGAAATTGCATTGAAATCCATTTTTATTGACCAATTAGAATTGTCTCCCAGGATCTATAATTGTCTTAAAAAGTCCAATATACATACATTATTGGAACTTTTGAATAAGAGTCAAGAAGACCTTATGAAAATTGAACATCTTCGCGTAGAAGATGTCAAAAATATATTAAACATTTTACAAATCGAAAAGCATTTTGCATAA

>MsG0380013939.01.T03

ATGAAAATTGAACATCTTCGCGTAGAAGATGTCAAAAATATATTAAACATTTTACAAATCGAAAAGCATTTTGCATAA

Protein sequence

>MsG0380013939.01.T01

LTPKEALYEASRNLIDLFIPFLHAEEENLNFENNQHKVTLPLFTFHDRLLKDKLRKNKKEIALKSIFIDQLELSPRIYNCLKKSNIHTLLELLNKSQEDLMKIEHLRVEDVKNILNILQIEKHFA*

>MsG0380013939.01.T02

NQHKVTLPLFTFHDRLLKDKLRKNKKEIALKSIFIDQLELSPRIYNCLKKSNIHTLLELLNKSQEDLMKIEHLRVEDVKNILNILQIEKHFA*

>MsG0380013939.01.T03

MKIEHLRVEDVKNILNILQIEKHFA*