Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014056.01.T01 | RYR62362.1 | 95.652 | 92 | 4 | 0 | 2 | 93 | 145 | 236 | 1.79E-56 | 184 |
| MsG0380014056.01.T03 | YP_009657390.1 | 100 | 49 | 0 | 0 | 1 | 49 | 44 | 92 | 3.71E-28 | 105 |
| MsG0380014056.01.T02 | YP_010173387.1 | 100 | 87 | 0 | 0 | 2 | 88 | 6 | 92 | 1.50E-56 | 179 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014056.01.T01 | P07816 | 94.253 | 87 | 5 | 0 | 7 | 93 | 6 | 92 | 3.02E-56 | 171 |
| MsG0380014056.01.T02 | P07816 | 94.253 | 87 | 5 | 0 | 2 | 88 | 6 | 92 | 2.79E-56 | 171 |
| MsG0380014056.01.T03 | A4GGF9 | 97.959 | 49 | 1 | 0 | 1 | 49 | 44 | 92 | 3.67E-30 | 103 |
| MsG0380014056.01.T04 | P07816 | 96.296 | 27 | 1 | 0 | 1 | 27 | 66 | 92 | 8.98E-13 | 58.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014056.01.T01 | A0A445DGW6 | 95.652 | 92 | 4 | 0 | 2 | 93 | 145 | 236 | 8.56e-57 | 184 |
| MsG0380014056.01.T02 | A0A898NBR1 | 100.000 | 87 | 0 | 0 | 2 | 88 | 6 | 92 | 7.16e-57 | 179 |
| MsG0380014056.01.T03 | A0A4P8F461 | 100.000 | 49 | 0 | 0 | 1 | 49 | 44 | 92 | 1.77e-28 | 105 |
| MsG0380014056.01.T04 | A0A898NBR1 | 100.000 | 27 | 0 | 0 | 1 | 27 | 66 | 92 | 6.13e-11 | 60.5 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049078.01 | MsG0380014056.01 | 0.812182 | 4.822920e-51 | 3.045386e-48 |
| MsG0080049079.01 | MsG0380014056.01 | 0.807120 | 5.902603e-50 | 3.260467e-47 |
| MsG0080049080.01 | MsG0380014056.01 | 0.808115 | 3.628308e-50 | 2.057071e-47 |
| MsG0380013927.01 | MsG0380014056.01 | 0.809472 | 1.860639e-50 | 1.093412e-47 |
| MsG0380013930.01 | MsG0380014056.01 | 0.812004 | 5.272403e-51 | 3.313153e-48 |
| MsG0380013932.01 | MsG0380014056.01 | 0.814440 | 1.539044e-51 | 1.032765e-48 |
| MsG0380013939.01 | MsG0380014056.01 | 0.838464 | 2.831212e-57 | 3.795215e-54 |
| MsG0380013955.01 | MsG0380014056.01 | 0.801266 | 9.764596e-49 | 4.643045e-46 |
| MsG0380013958.01 | MsG0380014056.01 | 0.829982 | 3.794807e-55 | 3.945864e-52 |
| MsG0380013959.01 | MsG0380014056.01 | 0.800569 | 1.355060e-48 | 6.330516e-46 |
| MsG0380013964.01 | MsG0380014056.01 | 0.801179 | 1.017554e-48 | 4.827835e-46 |
| MsG0380013965.01 | MsG0380014056.01 | 0.814050 | 1.876608e-51 | 1.245996e-48 |
| MsG0380013970.01 | MsG0380014056.01 | 0.812225 | 4.718763e-51 | 2.983261e-48 |
| MsG0380013979.01 | MsG0380014056.01 | 0.823178 | 1.592760e-53 | 1.361402e-50 |
| MsG0380013980.01 | MsG0380014056.01 | 0.820747 | 5.825085e-53 | 4.648712e-50 |
| MsG0380013988.01 | MsG0380014056.01 | 0.841327 | 5.082185e-58 | 7.441088e-55 |
| MsG0380013996.01 | MsG0380014056.01 | 0.831306 | 1.798602e-55 | 1.944145e-52 |
| MsG0380014009.01 | MsG0380014056.01 | 0.861939 | 7.212315e-64 | 2.075469e-60 |
| MsG0380014010.01 | MsG0380014056.01 | 0.929405 | 7.288835e-93 | 4.060933e-88 |
| MsG0380014012.01 | MsG0380014056.01 | 0.804151 | 2.478800e-49 | 1.268715e-46 |
| MsG0380014013.01 | MsG0380014056.01 | 0.826353 | 2.842447e-54 | 2.659309e-51 |
| MsG0380014022.01 | MsG0380014056.01 | 0.819117 | 1.374286e-52 | 1.048187e-49 |
| MsG0380014036.01 | MsG0380014056.01 | 0.809896 | 1.508506e-50 | 8.964598e-48 |
| MsG0380014037.01 | MsG0380014056.01 | 0.842162 | 3.059580e-58 | 4.598587e-55 |
| MsG0380014038.01 | MsG0380014056.01 | 0.824742 | 6.843992e-54 | 6.116192e-51 |
| MsG0380014041.01 | MsG0380014056.01 | 0.858067 | 1.062648e-62 | 2.678487e-59 |
| MsG0380014042.01 | MsG0380014056.01 | 0.963532 | 3.356886e-122 | 1.834488e-116 |
| MsG0380014055.01 | MsG0380014056.01 | 0.863545 | 2.305791e-64 | 7.015736e-61 |
| MsG0380014056.01 | MsG0380015733.01 | 0.801560 | 8.501668e-49 | 4.072495e-46 |
| MsG0380014056.01 | MsG0480020049.01 | 0.804185 | 2.438628e-49 | 1.249189e-46 |
| MsG0380014056.01 | MsG0580026430.01 | 0.800422 | 1.452295e-48 | 6.759750e-46 |
| MsG0380014056.01 | MsG0580028231.01 | 0.815223 | 1.032183e-51 | 7.074028e-49 |
| MsG0380014056.01 | MsG0680033966.01 | 0.810422 | 1.161899e-50 | 7.000508e-48 |
| MsG0380014056.01 | MsG0780037705.01 | 0.812465 | 4.182457e-51 | 2.661368e-48 |
| MsG0380014056.01 | MsG0880043541.01 | 0.813924 | 2.000391e-51 | 1.323648e-48 |
| MsG0380014056.01 | MsG0880044440.01 | 0.805879 | 1.078827e-49 | 5.772558e-47 |
| MsG0280007041.01 | MsG0380014056.01 | 0.817737 | 2.824249e-52 | 2.073750e-49 |
| MsG0280007042.01 | MsG0380014056.01 | 0.815619 | 8.424390e-52 | 5.837704e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014056.01.T04 | MTR_4g051420 | 96.296 | 27 | 1 | 0 | 1 | 27 | 66 | 92 | 1.46e-13 | 57.8 |
| MsG0380014056.01.T04 | MTR_3g035720 | 96.296 | 27 | 1 | 0 | 1 | 27 | 66 | 92 | 1.46e-13 | 57.8 |
| MsG0380014056.01.T04 | MTR_8g038480 | 92.593 | 27 | 2 | 0 | 1 | 27 | 54 | 80 | 6.45e-13 | 55.8 |
| MsG0380014056.01.T01 | MTR_4g051420 | 98.851 | 87 | 1 | 0 | 7 | 93 | 6 | 92 | 3.19e-59 | 176 |
| MsG0380014056.01.T01 | MTR_3g035720 | 98.851 | 87 | 1 | 0 | 7 | 93 | 6 | 92 | 3.19e-59 | 176 |
| MsG0380014056.01.T01 | MTR_0002s0800 | 62.366 | 93 | 30 | 2 | 3 | 93 | 127 | 216 | 2.44e-34 | 117 |
| MsG0380014056.01.T01 | MTR_0002s0360 | 62.366 | 93 | 30 | 2 | 3 | 93 | 127 | 216 | 2.44e-34 | 117 |
| MsG0380014056.01.T01 | MTR_8g038480 | 89.474 | 38 | 4 | 0 | 56 | 93 | 43 | 80 | 2.90e-20 | 77.4 |
| MsG0380014056.01.T03 | MTR_4g051420 | 97.959 | 49 | 1 | 0 | 1 | 49 | 44 | 92 | 4.59e-31 | 103 |
| MsG0380014056.01.T03 | MTR_3g035720 | 97.959 | 49 | 1 | 0 | 1 | 49 | 44 | 92 | 4.59e-31 | 103 |
| MsG0380014056.01.T03 | MTR_8g038480 | 89.474 | 38 | 4 | 0 | 12 | 49 | 43 | 80 | 6.96e-21 | 77.0 |
| MsG0380014056.01.T03 | MTR_0002s0800 | 68.627 | 51 | 14 | 1 | 1 | 49 | 166 | 216 | 3.29e-18 | 73.9 |
| MsG0380014056.01.T03 | MTR_0002s0360 | 68.627 | 51 | 14 | 1 | 1 | 49 | 166 | 216 | 3.29e-18 | 73.9 |
| MsG0380014056.01.T02 | MTR_4g051420 | 98.851 | 87 | 1 | 0 | 2 | 88 | 6 | 92 | 2.48e-59 | 176 |
| MsG0380014056.01.T02 | MTR_3g035720 | 98.851 | 87 | 1 | 0 | 2 | 88 | 6 | 92 | 2.48e-59 | 176 |
| MsG0380014056.01.T02 | MTR_0002s0800 | 67.073 | 82 | 25 | 1 | 9 | 88 | 135 | 216 | 2.24e-33 | 114 |
| MsG0380014056.01.T02 | MTR_0002s0360 | 67.073 | 82 | 25 | 1 | 9 | 88 | 135 | 216 | 2.24e-33 | 114 |
| MsG0380014056.01.T02 | MTR_8g038480 | 89.474 | 38 | 4 | 0 | 51 | 88 | 43 | 80 | 2.00e-20 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014056.01.T04 | ATCG00820 | 92.593 | 27 | 2 | 0 | 1 | 27 | 66 | 92 | 1.46e-13 | 58.2 |
| MsG0380014056.01.T01 | ATCG00820 | 87.356 | 87 | 11 | 0 | 7 | 93 | 6 | 92 | 2.64e-53 | 161 |
| MsG0380014056.01.T01 | AT5G47320 | 43.750 | 80 | 37 | 2 | 8 | 86 | 138 | 210 | 3.25e-12 | 60.1 |
| MsG0380014056.01.T03 | ATCG00820 | 87.755 | 49 | 6 | 0 | 1 | 49 | 44 | 92 | 2.64e-28 | 96.3 |
| MsG0380014056.01.T02 | ATCG00820 | 87.356 | 87 | 11 | 0 | 2 | 88 | 6 | 92 | 2.39e-53 | 161 |
| MsG0380014056.01.T02 | AT5G47320 | 43.750 | 80 | 37 | 2 | 3 | 81 | 138 | 210 | 2.48e-12 | 60.1 |
Find 18 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGAAAATTCTCCCAATTTA+TGG | 0.137320 | 3:+48747884 | None:intergenic |
| ATACGATCTGTTATATAAAT+AGG | 0.289134 | 3:+48747913 | None:intergenic |
| TCGTATGGTAGGCCATAAAT+TGG | 0.313687 | 3:-48747896 | MsG0380014056.01.T01:CDS |
| AGGTATAATGGTAGATGTTC+TGG | 0.324700 | 3:+48747984 | None:intergenic |
| CCCAATCATTGTAGGTATAA+TGG | 0.326942 | 3:+48747972 | None:intergenic |
| TTTCACCTACTCTAAACTTC+CGG | 0.337945 | 3:-48747867 | MsG0380014056.01.T01:CDS |
| ACCATTATACCTACAATGAT+TGG | 0.350564 | 3:-48747973 | MsG0380014056.01.T01:CDS |
| CATACCATTGCTATTCATAA+TGG | 0.354095 | 3:-48747949 | MsG0380014056.01.T01:CDS |
| TTCACCTACTCTAAACTTCC+GGG | 0.355100 | 3:-48747866 | MsG0380014056.01.T01:CDS |
| AATAAATGATTCGCTACAAA+AGG | 0.410516 | 3:+48748063 | None:intergenic |
| ATGGTATGCCCAATCATTGT+AGG | 0.463349 | 3:+48747964 | None:intergenic |
| ATAAATAAGCTTAACACAAA+AGG | 0.463559 | 3:-48748033 | MsG0380014056.01.T01:CDS |
| CCATTATACCTACAATGATT+GGG | 0.487902 | 3:-48747972 | MsG0380014056.01.T01:CDS |
| CGTATGGTAGGCCATAAATT+GGG | 0.512944 | 3:-48747895 | MsG0380014056.01.T01:CDS |
| TATATAACAGATCGTATGGT+AGG | 0.535301 | 3:-48747907 | MsG0380014056.01.T01:CDS |
| TATTTATATAACAGATCGTA+TGG | 0.571298 | 3:-48747911 | MsG0380014056.01.T01:CDS |
| CACCTACTCTAAACTTCCGG+GGG | 0.635563 | 3:-48747864 | MsG0380014056.01.T01:CDS |
| TCACCTACTCTAAACTTCCG+GGG | 0.640846 | 3:-48747865 | MsG0380014056.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAGAAATAATAATAACT+TGG | - | Chr3:48747913-48747932 | MsG0380014056.01.T01:CDS | 10.0% |
| !! | ATAAATAAGCTTAACACAAA+AGG | - | Chr3:48747888-48747907 | MsG0380014056.01.T01:CDS | 20.0% |
| !! | ATACGATCTGTTATATAAAT+AGG | + | Chr3:48748011-48748030 | None:intergenic | 20.0% |
| !! | TATTTATATAACAGATCGTA+TGG | - | Chr3:48748010-48748029 | MsG0380014056.01.T01:CDS | 20.0% |
| ! | AATAAATGATTCGCTACAAA+AGG | + | Chr3:48747861-48747880 | None:intergenic | 25.0% |
| !! | TTTTCCATTATGAATAGCAA+TGG | + | Chr3:48747979-48747998 | None:intergenic | 25.0% |
| ACCATTATACCTACAATGAT+TGG | - | Chr3:48747948-48747967 | MsG0380014056.01.T01:CDS | 30.0% | |
| CCATTATACCTACAATGATT+GGG | - | Chr3:48747949-48747968 | MsG0380014056.01.T01:CDS | 30.0% | |
| GTGAAAATTCTCCCAATTTA+TGG | + | Chr3:48748040-48748059 | None:intergenic | 30.0% | |
| TATATAACAGATCGTATGGT+AGG | - | Chr3:48748014-48748033 | MsG0380014056.01.T01:CDS | 30.0% | |
| ! | CATACCATTGCTATTCATAA+TGG | - | Chr3:48747972-48747991 | MsG0380014056.01.T01:CDS | 30.0% |
| AGGTATAATGGTAGATGTTC+TGG | + | Chr3:48747940-48747959 | None:intergenic | 35.0% | |
| CCCAATCATTGTAGGTATAA+TGG | + | Chr3:48747952-48747971 | None:intergenic | 35.0% | |
| TTTCACCTACTCTAAACTTC+CGG | - | Chr3:48748054-48748073 | MsG0380014056.01.T01:CDS | 35.0% | |
| ATGGTATGCCCAATCATTGT+AGG | + | Chr3:48747960-48747979 | None:intergenic | 40.0% | |
| CGTATGGTAGGCCATAAATT+GGG | - | Chr3:48748026-48748045 | MsG0380014056.01.T01:CDS | 40.0% | |
| TCGTATGGTAGGCCATAAAT+TGG | - | Chr3:48748025-48748044 | MsG0380014056.01.T01:CDS | 40.0% | |
| TTCACCTACTCTAAACTTCC+GGG | - | Chr3:48748055-48748074 | MsG0380014056.01.T01:CDS | 40.0% | |
| !! | TATCATTTTTCGCATACCCC+CGG | + | Chr3:48748076-48748095 | None:intergenic | 40.0% |
| TCACCTACTCTAAACTTCCG+GGG | - | Chr3:48748056-48748075 | MsG0380014056.01.T01:CDS | 45.0% | |
| CACCTACTCTAAACTTCCGG+GGG | - | Chr3:48748057-48748076 | MsG0380014056.01.T01:CDS | 50.0% | |
| ! | TACCCCCGGAAGTTTAGAGT+AGG | + | Chr3:48748062-48748081 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48747831 | 48748112 | 48747831 | ID=MsG0380014056.01;Name=MsG0380014056.01 |
| Chr3 | mRNA | 48747831 | 48748112 | 48747831 | ID=MsG0380014056.01.T01;Parent=MsG0380014056.01;Name=MsG0380014056.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|93 |
| Chr3 | exon | 48747831 | 48748112 | 48747831 | ID=MsG0380014056.01.T01:exon:9384;Parent=MsG0380014056.01.T01 |
| Chr3 | CDS | 48747831 | 48748112 | 48747831 | ID=MsG0380014056.01.T01:cds;Parent=MsG0380014056.01.T01 |
| Chr3 | mRNA | 48747831 | 48748097 | 48747831 | ID=MsG0380014056.01.T02;Parent=MsG0380014056.01;Name=MsG0380014056.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|88 |
| Chr3 | exon | 48747831 | 48748097 | 48747831 | ID=MsG0380014056.01.T02:exon:9385;Parent=MsG0380014056.01.T02 |
| Chr3 | CDS | 48747831 | 48748097 | 48747831 | ID=MsG0380014056.01.T02:cds;Parent=MsG0380014056.01.T02 |
| Chr3 | mRNA | 48747831 | 48747980 | 48747831 | ID=MsG0380014056.01.T03;Parent=MsG0380014056.01;Name=MsG0380014056.01.T03;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|49 |
| Chr3 | exon | 48747831 | 48747980 | 48747831 | ID=MsG0380014056.01.T03:exon:9386;Parent=MsG0380014056.01.T03 |
| Chr3 | CDS | 48747831 | 48747980 | 48747831 | ID=MsG0380014056.01.T03:cds;Parent=MsG0380014056.01.T03 |
| Chr3 | mRNA | 48747831 | 48747914 | 48747831 | ID=MsG0380014056.01.T04;Parent=MsG0380014056.01;Name=MsG0380014056.01.T04;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|27 |
| Chr3 | exon | 48747831 | 48747914 | 48747831 | ID=MsG0380014056.01.T04:exon:9387;Parent=MsG0380014056.01.T04 |
| Chr3 | CDS | 48747831 | 48747914 | 48747831 | ID=MsG0380014056.01.T04:cds;Parent=MsG0380014056.01.T04 |
| Gene Sequence |
| Protein sequence |
