AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027650.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027650.01.T01 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0580027650.01.T01 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0580027650.01.T01 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0580027650.01.T01 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0580027650.01.T01 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0580027650.01.T01 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0580027650.01.T01 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0580027650.01.T01 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0580027650.01.T01 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0580027650.01.T01 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0580027650.01.T01 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0580027650.01.T01 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0580027650.01.T01 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0580027650.01.T01 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0580027650.01.T01 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.74e-66 204
MsG0580027650.01.T01 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.23e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027650.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0580027650.01.T01 AT5G59690 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0580027650.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0580027650.01.T01 AT3G46320 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0580027650.01.T01 AT3G45930 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0580027650.01.T01 AT3G53730 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0580027650.01.T01 AT2G28740 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0580027650.01.T01 AT1G07660 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0580027650.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0580027650.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0580027650.01.T01 AT1G07660 83.495 103 0 1 1 103 1 86 4.52e-51 155

Find 26 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAGGACCCTTTATGGTTT+TGG 0.268415 5:-65188221 MsG0580027650.01.T01:CDS
AGACAAGGAAGGACCCTTTA+TGG 0.291085 5:-65188227 MsG0580027650.01.T01:CDS
CCTAACCACCAAAACCATAA+AGG 0.346431 5:+65188213 None:intergenic
AAGACGACGAATCGCGGGTT+TGG 0.381861 5:+65188412 None:intergenic
GGCAAAGGAGGAAAGGGTTT+AGG 0.384869 5:-65188491 MsG0580027650.01.T01:CDS
GTTCTTCGAGATAACATTCA+AGG 0.421661 5:-65188440 MsG0580027650.01.T01:CDS
CTAACCACCAAAACCATAAA+GGG 0.435105 5:+65188214 None:intergenic
GGAGGAAAGGGTTTAGGAAA+GGG 0.452389 5:-65188485 MsG0580027650.01.T01:CDS
CCGCGATTCGTCGTCTTGCT+AGG 0.461756 5:-65188406 MsG0580027650.01.T01:CDS
ATCTTGCAATCAATCATGTC+AGG 0.507815 5:-65188518 None:intergenic
GGAAAGGGTTTAGGAAAGGG+TGG 0.509385 5:-65188482 MsG0580027650.01.T01:CDS
AGGAAGAGGCAAAGGAGGAA+AGG 0.518412 5:-65188498 MsG0580027650.01.T01:CDS
GGAAGAGGCAAAGGAGGAAA+GGG 0.527098 5:-65188497 MsG0580027650.01.T01:CDS
TCGTAAAACAGTTACTGCTA+TGG 0.534488 5:-65188270 MsG0580027650.01.T01:CDS
AGGAGGAAAGGGTTTAGGAA+AGG 0.547494 5:-65188486 MsG0580027650.01.T01:CDS
CAATCAATCATGTCAGGAAG+AGG 0.559993 5:-65188512 None:intergenic
CCTAGCAAGACGACGAATCG+CGG 0.578097 5:+65188406 None:intergenic
ATCATGTCAGGAAGAGGCAA+AGG 0.584537 5:-65188506 MsG0580027650.01.T01:CDS
CTAGCAAGACGACGAATCGC+GGG 0.586259 5:+65188407 None:intergenic
ATGTCAGGAAGAGGCAAAGG+AGG 0.604597 5:-65188503 MsG0580027650.01.T01:CDS
CGTCGTCTTGCTAGGCGTGG+TGG 0.633047 5:-65188398 MsG0580027650.01.T01:CDS
ATTCGTCGTCTTGCTAGGCG+TGG 0.638134 5:-65188401 MsG0580027650.01.T01:CDS
GGTGGTGTAAAGCGTATCAG+TGG 0.649124 5:-65188380 MsG0580027650.01.T01:CDS
GTTTATGCACTCAAGAGACA+AGG 0.649593 5:-65188242 MsG0580027650.01.T01:CDS
ATGCACTCAAGAGACAAGGA+AGG 0.704360 5:-65188238 MsG0580027650.01.T01:CDS
TTGATCTATGAAGAGACTCG+TGG 0.728425 5:-65188356 MsG0580027650.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CTAACCACCAAAACCATAAA+GGG + Chr5:65188506-65188525 None:intergenic 35.0%
GTTCTTCGAGATAACATTCA+AGG - Chr5:65188277-65188296 MsG0580027650.01.T01:CDS 35.0%
TCGTAAAACAGTTACTGCTA+TGG - Chr5:65188447-65188466 MsG0580027650.01.T01:CDS 35.0%
GTTTATGCACTCAAGAGACA+AGG - Chr5:65188475-65188494 MsG0580027650.01.T01:CDS 40.0%
TTGATCTATGAAGAGACTCG+TGG - Chr5:65188361-65188380 MsG0580027650.01.T01:CDS 40.0%
AGACAAGGAAGGACCCTTTA+TGG - Chr5:65188490-65188509 MsG0580027650.01.T01:CDS 45.0%
AGGAGGAAAGGGTTTAGGAA+AGG - Chr5:65188231-65188250 MsG0580027650.01.T01:CDS 45.0%
ATGCACTCAAGAGACAAGGA+AGG - Chr5:65188479-65188498 MsG0580027650.01.T01:CDS 45.0%
GGAGGAAAGGGTTTAGGAAA+GGG - Chr5:65188232-65188251 MsG0580027650.01.T01:CDS 45.0%
! GGAAGGACCCTTTATGGTTT+TGG - Chr5:65188496-65188515 MsG0580027650.01.T01:CDS 45.0%
!!! AGGACCCTTTATGGTTTTGG+TGG - Chr5:65188499-65188518 MsG0580027650.01.T01:CDS 45.0%
AGGAAGAGGCAAAGGAGGAA+AGG - Chr5:65188219-65188238 MsG0580027650.01.T01:CDS 50.0%
ATGTCAGGAAGAGGCAAAGG+AGG - Chr5:65188214-65188233 MsG0580027650.01.T01:CDS 50.0%
GGAAAGGGTTTAGGAAAGGG+TGG - Chr5:65188235-65188254 MsG0580027650.01.T01:CDS 50.0%
GGAAGAGGCAAAGGAGGAAA+GGG - Chr5:65188220-65188239 MsG0580027650.01.T01:CDS 50.0%
GGCAAAGGAGGAAAGGGTTT+AGG - Chr5:65188226-65188245 MsG0580027650.01.T01:CDS 50.0%
GGTGGTGTAAAGCGTATCAG+TGG - Chr5:65188337-65188356 MsG0580027650.01.T01:CDS 50.0%
AAGACGACGAATCGCGGGTT+TGG + Chr5:65188308-65188327 None:intergenic 55.0%
ATTCGTCGTCTTGCTAGGCG+TGG - Chr5:65188316-65188335 MsG0580027650.01.T01:CDS 55.0%
CCTAGCAAGACGACGAATCG+CGG + Chr5:65188314-65188333 None:intergenic 55.0%
CTAGCAAGACGACGAATCGC+GGG + Chr5:65188313-65188332 None:intergenic 55.0%
CCGCGATTCGTCGTCTTGCT+AGG - Chr5:65188311-65188330 MsG0580027650.01.T01:CDS 60.0%
CGTCGTCTTGCTAGGCGTGG+TGG - Chr5:65188319-65188338 MsG0580027650.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 65188214 65188525 65188214 ID=MsG0580027650.01;Name=MsG0580027650.01
Chr5 mRNA 65188214 65188525 65188214 ID=MsG0580027650.01.T01;Parent=MsG0580027650.01;Name=MsG0580027650.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|103
Chr5 exon 65188214 65188525 65188214 ID=MsG0580027650.01.T01:exon:25343;Parent=MsG0580027650.01.T01
Chr5 CDS 65188214 65188525 65188214 ID=MsG0580027650.01.T01:cds;Parent=MsG0580027650.01.T01
Gene Sequence

>MsG0580027650.01.T01

ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTTTAGGAAAGGGTGGAGCAAAGCGTCATCGCAAAGTTCTTCGAGATAACATTCAAGGAATAACCAAACCCGCGATTCGTCGTCTTGCTAGGCGTGGTGGTGTAAAGCGTATCAGTGGTTTGATCTATGAAGAGACTCGTGGTGTTCTCAAGATATTTCTTGAGAATGTTATTCGTGATGCTGTAACTTACACTGAACATGCTCGTCGTAAAACAGTTACTGCTATGGATGTTGTTTATGCACTCAAGAGACAAGGAAGGACCCTTTATGGTTTTGGTGGTTAG

Protein sequence

>MsG0580027650.01.T01

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*