AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031731.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031731.01.T01 MTR_3g096690 50.000 106 40 2 83 175 2 107 1.90e-26 97.1
MsG0680031731.01.T01 MTR_0021s0240 46.479 71 34 1 106 172 87 157 1.95e-13 65.1
MsG0680031731.01.T01 MTR_8g445550 43.662 71 36 1 106 172 49 119 1.80e-12 61.2
MsG0680031731.01.T01 MTR_8g445560 43.662 71 36 1 106 172 49 119 1.80e-12 61.2
MsG0680031731.01.T01 MTR_8g099650 39.474 76 43 1 99 171 87 162 3.67e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031731.01.T01 AT5G09250 44.828 87 40 1 95 173 19 105 1.59e-19 79.3
MsG0680031731.01.T01 AT5G09250 45.833 72 31 1 95 158 19 90 7.62e-16 70.1
MsG0680031731.01.T01 AT5G09250 46.269 67 36 0 109 175 44 110 3.94e-14 65.5
MsG0680031731.01.T01 AT5G09250 46.970 66 27 1 95 152 19 84 6.07e-14 64.3
MsG0680031731.01.T01 AT5G09250 46.970 66 27 1 95 152 19 84 6.07e-14 64.3
MsG0680031731.01.T01 AT5G09240 38.372 86 46 2 95 173 21 106 4.80e-12 60.1

Find 44 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCACTCAATGAGGACTCTT+AGG 0.366094 6:-25490859 None:intergenic
CGCGAATATTACTTAAAAGA+TGG 0.404513 6:-25493480 MsG0680031731.01.T01:CDS
AGCATTGGAACGAAGGGATT+CGG 0.424874 6:-25493512 MsG0680031731.01.T01:CDS
AATAGGTCTGGAACTGAAAA+GGG 0.449012 6:-25493722 MsG0680031731.01.T01:CDS
GAAGAGGTCTGCAACTGAAA+AGG 0.458649 6:-25493750 MsG0680031731.01.T01:CDS
GAAGAGGTCTGCAACTGAAA+AGG 0.458649 6:-25493804 MsG0680031731.01.T01:CDS
GAAGAGGTCTGCAACTGAAA+AGG 0.458649 6:-25493858 MsG0680031731.01.T01:CDS
CAACTGAAAAGGAGAAGAAT+AGG 0.461723 6:-25493739 MsG0680031731.01.T01:CDS
CAACTGAAAAGGAGAAGAAT+AGG 0.461723 6:-25493793 MsG0680031731.01.T01:CDS
CAACTGAAAAGGAGAAGAAT+AGG 0.461723 6:-25493847 MsG0680031731.01.T01:CDS
GAATAGGTCTGGAACTGAAA+AGG 0.466779 6:-25493723 MsG0680031731.01.T01:CDS
GAACTGAAAAGGAGAAGAAT+AGG 0.468877 6:-25493901 MsG0680031731.01.T01:CDS
GAACTGAAAAGGAGAAGAAT+AGG 0.468877 6:-25493955 MsG0680031731.01.T01:CDS
AGAAATGCCTGGAACTGAAA+AGG 0.469309 6:-25493966 None:intergenic
GAATAGGTCTGGAACTGAAA+AGG 0.472159 6:-25493777 MsG0680031731.01.T01:CDS
GAATAGGTCTGGAACTGAAA+AGG 0.472159 6:-25493831 MsG0680031731.01.T01:CDS
GAATAGGTCTGGAACTGAAA+AGG 0.472159 6:-25493885 MsG0680031731.01.T01:CDS
GAATAGGTCTGGAACTGAAA+AGG 0.472159 6:-25493939 MsG0680031731.01.T01:CDS
GAAAAGGAGAAGAATAGGTC+TGG 0.477457 6:-25493734 MsG0680031731.01.T01:CDS
GAAAAGGAGAAGAATAGGTC+TGG 0.477457 6:-25493788 MsG0680031731.01.T01:CDS
GAAAAGGAGAAGAATAGGTC+TGG 0.477457 6:-25493842 MsG0680031731.01.T01:CDS
GAAAAGGAGAAGAATAGGTC+TGG 0.477457 6:-25493896 MsG0680031731.01.T01:CDS
GAAAAGGAGAAGAATAGGTC+TGG 0.477457 6:-25493950 MsG0680031731.01.T01:CDS
GAAAAGGAGAAGAAGTGGTC+TGG 0.481785 6:-25493923 MsG0680031731.01.T01:CDS
GGAAGGTTTCTGTGAAGCAT+TGG 0.486433 6:-25493527 MsG0680031731.01.T01:CDS
AATCTTTATAGAGAAATGCC+TGG 0.524021 6:-25493977 None:intergenic
CTGTGAAGCATTGGAACGAA+GGG 0.525495 6:-25493518 MsG0680031731.01.T01:CDS
GAAGTGGTCTGGAACTGAAA+AGG 0.529990 6:-25493912 MsG0680031731.01.T01:CDS
GACCATGTCGGAGTTGATGA+TGG 0.532598 6:-25490910 MsG0680031731.01.T01:CDS
GAACTGAAAAGGAGAAGAAG+AGG 0.574608 6:-25493766 MsG0680031731.01.T01:CDS
GAACTGAAAAGGAGAAGAAG+AGG 0.574608 6:-25493820 MsG0680031731.01.T01:CDS
GAACTGAAAAGGAGAAGAAG+AGG 0.574608 6:-25493874 MsG0680031731.01.T01:CDS
CGACGAAATCGTCGTATGTG+AGG 0.579073 6:-25493645 MsG0680031731.01.T01:intron
GAACTGAAAAGGGGAAGAAG+AGG 0.586147 6:-25493712 MsG0680031731.01.T01:CDS
TCTGTGAAGCATTGGAACGA+AGG 0.589293 6:-25493519 MsG0680031731.01.T01:CDS
GAACTGAAAAGGAGAAGAAG+TGG 0.598598 6:-25493928 MsG0680031731.01.T01:CDS
CAACTCCGACATGGTCAATG+AGG 0.600146 6:+25490917 None:intergenic
ATGATGGTTCGTAATCACAT+TGG 0.601069 6:-25490894 MsG0680031731.01.T01:CDS
GCAGCTTGGGACAAAGAGGA+AGG 0.602778 6:-25493544 MsG0680031731.01.T01:intron
TAACGCAGCTTGGGACAAAG+AGG 0.608874 6:-25493548 MsG0680031731.01.T01:intron
AATTGACAATGCACTCAATG+AGG 0.616344 6:-25490868 MsG0680031731.01.T01:CDS
AACCATCATCAACTCCGACA+TGG 0.642168 6:+25490908 None:intergenic
AGGAACCTCATTGACCATGT+CGG 0.644511 6:-25490922 MsG0680031731.01.T01:CDS
ATAGGTCTGGAACTGAAAAG+GGG 0.667488 6:-25493721 MsG0680031731.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTAATAATACAACTAA+AGG - Chr6:25492446-25492465 MsG0680031731.01.T01:intron 10.0%
!!! TTTTTAATAATACAACTAAA+GGG - Chr6:25492447-25492466 MsG0680031731.01.T01:intron 10.0%
!! AAATTTCCATAAAATTGAAT+TGG + Chr6:25491534-25491553 None:intergenic 15.0%
!! AGTAATTGTAATCTTAATTT+CGG + Chr6:25492998-25493017 None:intergenic 15.0%
!! TCAATCACTTTATATAAATA+AGG + Chr6:25491443-25491462 None:intergenic 15.0%
!! TTATGAAATGTTGTTATAAT+GGG - Chr6:25491411-25491430 MsG0680031731.01.T01:intron 15.0%
!! TTTATGAAATGTTGTTATAA+TGG - Chr6:25491410-25491429 MsG0680031731.01.T01:intron 15.0%
!!! AATTCAATTTTATGGAAATT+TGG - Chr6:25491533-25491552 MsG0680031731.01.T01:intron 15.0%
!!! ATTTATTAAGTTTTTGTTCT+TGG - Chr6:25493756-25493775 MsG0680031731.01.T01:CDS 15.0%
!!! TTTTAATAATACAACTAAAG+GGG - Chr6:25492448-25492467 MsG0680031731.01.T01:intron 15.0%
!!! TTTTTAAAGAATAATGATGT+TGG + Chr6:25493128-25493147 None:intergenic 15.0%
!! CAAAATTGATAAACAAAATC+AGG + Chr6:25491501-25491520 None:intergenic 20.0%
!!! AAATGTGAGATTTGTTAATT+TGG + Chr6:25491927-25491946 None:intergenic 20.0%
!!! ATGAATTGAAATACCTTTTT+TGG + Chr6:25491382-25491401 None:intergenic 20.0%
!!! TATATGTAGAATTGCATTTT+GGG - Chr6:25492207-25492226 MsG0680031731.01.T01:intron 20.0%
!!! TGAATGTTGGATTTTTATTT+GGG + Chr6:25492030-25492049 None:intergenic 20.0%
!!! TGGTTTCTAGTTATTTTTTT+CGG + Chr6:25491961-25491980 None:intergenic 20.0%
!!! TTATATGTAGAATTGCATTT+TGG - Chr6:25492206-25492225 MsG0680031731.01.T01:intron 20.0%
!!! TTGAATGTTGGATTTTTATT+TGG + Chr6:25492031-25492050 None:intergenic 20.0%
!!! TTTAGAATGAATCAATGAAA+CGG + Chr6:25493317-25493336 None:intergenic 20.0%
! AAATAACAACAAGCACTTTA+AGG - Chr6:25491980-25491999 MsG0680031731.01.T01:intron 25.0%
! ACATTTGTATACTTTCTTTC+AGG - Chr6:25493880-25493899 MsG0680031731.01.T01:CDS 25.0%
! AGGAAGTGAAAGTTATTTAT+TGG - Chr6:25492486-25492505 MsG0680031731.01.T01:intron 25.0%
! ATGTTTAATGGAATAGCAAA+TGG - Chr6:25491467-25491486 MsG0680031731.01.T01:intron 25.0%
! CTGTTATGGTACATTATTAT+GGG + Chr6:25491855-25491874 None:intergenic 25.0%
! GTGATTGATGAAATGTTTAA+TGG - Chr6:25491455-25491474 MsG0680031731.01.T01:intron 25.0%
! TCTGTTATGGTACATTATTA+TGG + Chr6:25491856-25491875 None:intergenic 25.0%
! TGACAGTGAAATTGTTTATA+TGG - Chr6:25492051-25492070 MsG0680031731.01.T01:intron 25.0%
! TTAAAGAATAATGATGTTGG+TGG + Chr6:25493125-25493144 None:intergenic 25.0%
! TTACAATTACTTCCTTATCA+TGG - Chr6:25493007-25493026 MsG0680031731.01.T01:intron 25.0%
!! ACAATTAAGGATTGGTAATA+AGG + Chr6:25493522-25493541 None:intergenic 25.0%
!! ATATGTAGAATTGCATTTTG+GGG - Chr6:25492208-25492227 MsG0680031731.01.T01:intron 25.0%
!! CTTAGTAATATCTAGAACTT+TGG - Chr6:25492075-25492094 MsG0680031731.01.T01:intron 25.0%
!! TGATTCATTCTAAAGAGATA+AGG - Chr6:25493323-25493342 MsG0680031731.01.T01:intron 25.0%
!! TTGTCACCAATTCAATTTTA+TGG - Chr6:25491525-25491544 MsG0680031731.01.T01:intron 25.0%
!!! ATTGAAATACCTTTTTTGGT+AGG + Chr6:25491378-25491397 None:intergenic 25.0%
!!! CGACAAAGGTTTTTAAAAAA+AGG - Chr6:25491606-25491625 MsG0680031731.01.T01:intron 25.0%
!!! GAATGTTGGATTTTTATTTG+GGG + Chr6:25492029-25492048 None:intergenic 25.0%
!!! TTAAAGTGCTTGTTGTTATT+TGG + Chr6:25491981-25492000 None:intergenic 25.0%
AATGCAATTCTTAGAATCCT+TGG + Chr6:25492169-25492188 None:intergenic 30.0%
ATACAGAGACAATTAAGGAT+TGG + Chr6:25493530-25493549 None:intergenic 30.0%
ATAGAGAAAGGAGATGTATA+AGG + Chr6:25491798-25491817 None:intergenic 30.0%
ATTTCACTGTCATTGAATGT+TGG + Chr6:25492043-25492062 None:intergenic 30.0%
ATTTGTGTTTACTAGCCAAA+TGG - Chr6:25492252-25492271 MsG0680031731.01.T01:intron 30.0%
CAGTAATACAGAGACAATTA+AGG + Chr6:25493535-25493554 None:intergenic 30.0%
CATTATCTTAGAAGTTGTGA+TGG - Chr6:25493373-25493392 MsG0680031731.01.T01:intron 30.0%
CGCGAATATTACTTAAAAGA+TGG - Chr6:25491342-25491361 MsG0680031731.01.T01:intron 30.0%
GACACACCTAAAAGTAAATT+GGG + Chr6:25493608-25493627 None:intergenic 30.0%
TGAAAGTTATTTATTGGCGA+TGG - Chr6:25492492-25492511 MsG0680031731.01.T01:intron 30.0%
TGATGAAATTCAAAAGCCTA+AGG - Chr6:25491582-25491601 MsG0680031731.01.T01:intron 30.0%
TTTGAGTAATTGTGATTGAG+AGG - Chr6:25493261-25493280 MsG0680031731.01.T01:intron 30.0%
! CAATTTTCAAACCTCTCAAA+TGG + Chr6:25493350-25493369 None:intergenic 30.0%
! CTGGTCTATTTTGTGTTTTA+GGG - Chr6:25492683-25492702 MsG0680031731.01.T01:intron 30.0%
! GTTGGTGGAGAAATATAATA+CGG - Chr6:25492320-25492339 MsG0680031731.01.T01:intron 30.0%
! TATTTATTGGCGATGGATTT+GGG - Chr6:25492499-25492518 MsG0680031731.01.T01:intron 30.0%
! TGGTGCATCATAATATTTTG+TGG + Chr6:25491769-25491788 None:intergenic 30.0%
! TTATTTATTGGCGATGGATT+TGG - Chr6:25492498-25492517 MsG0680031731.01.T01:intron 30.0%
!! CATCACAACTTCTAAGATAA+TGG + Chr6:25493375-25493394 None:intergenic 30.0%
!! CTTTGACCCAATTTACTTTT+AGG - Chr6:25493599-25493618 MsG0680031731.01.T01:intron 30.0%
!! GATTTGTTAATTTGGTAGGA+AGG + Chr6:25491919-25491938 None:intergenic 30.0%
!! GTGAGATTTGTTAATTTGGT+AGG + Chr6:25491923-25491942 None:intergenic 30.0%
!! TGGTCTATTTTGTGTTTTAG+GGG - Chr6:25492684-25492703 MsG0680031731.01.T01:intron 30.0%
!! TTTGAGATCTTGGTTGTTTT+TGG - Chr6:25493227-25493246 MsG0680031731.01.T01:intron 30.0%
!!! AATTGTTTTTGGACTACTTG+CGG - Chr6:25492623-25492642 MsG0680031731.01.T01:intron 30.0%
!!! TTGAGATCTTGGTTGTTTTT+GGG - Chr6:25493228-25493247 MsG0680031731.01.T01:intron 30.0%
AAATTCGCGAATGAAGAAAC+TGG + Chr6:25492890-25492909 None:intergenic 35.0%
AAGCAAAGACCTACCAAAAA+AGG - Chr6:25491366-25491385 MsG0680031731.01.T01:intron 35.0%
AATGCAACCATTATTAGACC+TGG + Chr6:25493095-25493114 None:intergenic 35.0%
AATTGACAATGCACTCAATG+AGG - Chr6:25493954-25493973 MsG0680031731.01.T01:CDS 35.0%
ACCATTCGAGAAAGAATCAT+GGG + Chr6:25493190-25493209 None:intergenic 35.0%
ACTTCCTACAGAATCTGTTA+TGG + Chr6:25491869-25491888 None:intergenic 35.0%
AGGACAAATACGAATGGAAT+TGG - Chr6:25492525-25492544 MsG0680031731.01.T01:intron 35.0%
ATGAGAAGAGAGATAAAGCT+GGG + Chr6:25491821-25491840 None:intergenic 35.0%
ATGATGGTTCGTAATCACAT+TGG - Chr6:25493928-25493947 MsG0680031731.01.T01:CDS 35.0%
ATGCAACCATTATTAGACCT+GGG + Chr6:25493094-25493113 None:intergenic 35.0%
ATGGAATAGCAAATGGACAT+TGG - Chr6:25491474-25491493 MsG0680031731.01.T01:intron 35.0%
CAACTGAAAAGGAGAAGAAT+AGG - Chr6:25490975-25490994 MsG0680031731.01.T01:intron 35.0%
GAAATTCCCAGGTCTAATAA+TGG - Chr6:25493085-25493104 MsG0680031731.01.T01:intron 35.0%
GAACTGAAAAGGAGAAGAAT+AGG - Chr6:25490867-25490886 MsG0680031731.01.T01:CDS 35.0%
GGACACACCTAAAAGTAAAT+TGG + Chr6:25493609-25493628 None:intergenic 35.0%
GTCGTATATACAATCTTCCA+CGG + Chr6:25491732-25491751 None:intergenic 35.0%
TAAAAACCTTTGTCGTCCTT+AGG + Chr6:25491601-25491620 None:intergenic 35.0%
TACCATTCGAGAAAGAATCA+TGG + Chr6:25493191-25493210 None:intergenic 35.0%
TAGATGGAGAGAAAGAGATA+CGG - Chr6:25493416-25493435 MsG0680031731.01.T01:intron 35.0%
TATCTTAGAAGTTGTGATGG+TGG - Chr6:25493376-25493395 MsG0680031731.01.T01:intron 35.0%
TCTTTCTCGAATGGTATCAA+CGG - Chr6:25493195-25493214 MsG0680031731.01.T01:intron 35.0%
TGTACCATAACAGATTCTGT+AGG - Chr6:25491862-25491881 MsG0680031731.01.T01:intron 35.0%
TGTGATTGAGAGGAAAAGAA+AGG - Chr6:25493271-25493290 MsG0680031731.01.T01:intron 35.0%
TTACAGTTGCATCCATGATA+AGG + Chr6:25493022-25493041 None:intergenic 35.0%
TTATTCGATCCAAAAACCGA+AGG + Chr6:25493630-25493649 None:intergenic 35.0%
! AACTCACTGTTTTACATGAC+AGG + Chr6:25492729-25492748 None:intergenic 35.0%
! AAGAATAATGATGTTGGTGG+TGG + Chr6:25493122-25493141 None:intergenic 35.0%
! AATAGGTCTGGAACTGAAAA+GGG - Chr6:25490884-25490903 MsG0680031731.01.T01:CDS 35.0%
! ATTTATTGGCGATGGATTTG+GGG - Chr6:25492500-25492519 MsG0680031731.01.T01:intron 35.0%
! TGTTAATTTGGTAGGAAGGT+TGG + Chr6:25491915-25491934 None:intergenic 35.0%
!! AGCTGATTTCCTTTCCTTTT+AGG - Chr6:25492774-25492793 MsG0680031731.01.T01:intron 35.0%
!! AGTTTTAGTGCTTGAAGGTT+GGG - Chr6:25492583-25492602 MsG0680031731.01.T01:intron 35.0%
!! GCTGGTCTATTTTGTGTTTT+AGG - Chr6:25492682-25492701 MsG0680031731.01.T01:intron 35.0%
!! GGTCTATTTTGTGTTTTAGG+GGG - Chr6:25492685-25492704 MsG0680031731.01.T01:intron 35.0%
!! TGATTTGTTTAACGCAGCTT+GGG - Chr6:25491265-25491284 MsG0680031731.01.T01:intron 35.0%
!! TTACTTTTAGGTGTGTCCTT+CGG - Chr6:25493611-25493630 MsG0680031731.01.T01:intron 35.0%
!! TTGATTTGTTTAACGCAGCT+TGG - Chr6:25491264-25491283 MsG0680031731.01.T01:intron 35.0%
!!! GTTCGTAGTTTTTGAGATCT+TGG - Chr6:25493217-25493236 MsG0680031731.01.T01:intron 35.0%
!!! TTGGTTGTTTTTGGGTGAAT+CGG - Chr6:25493236-25493255 MsG0680031731.01.T01:intron 35.0%
AAAGGAGATGTATAAGGCGT+TGG + Chr6:25491792-25491811 None:intergenic 40.0%
AAGAACCTTGTGAAATTCCC+AGG - Chr6:25493074-25493093 MsG0680031731.01.T01:intron 40.0%
AAGAACTTCTCACTGTCTGT+CGG + Chr6:25492142-25492161 None:intergenic 40.0%
ACCCATGATTCTTTCTCGAA+TGG - Chr6:25493186-25493205 MsG0680031731.01.T01:intron 40.0%
AGACTGAGCCTGGTAATAAA+GGG + Chr6:25491681-25491700 None:intergenic 40.0%
AGAGATAAGGTCCATTTGAG+AGG - Chr6:25493336-25493355 MsG0680031731.01.T01:intron 40.0%
AGCCTGGTAATAAAGGGAAA+GGG + Chr6:25491675-25491694 None:intergenic 40.0%
CAAACATCATCCAACTTGGT+GGG + Chr6:25493823-25493842 None:intergenic 40.0%
CATGAGAAGAGAGATAAAGC+TGG + Chr6:25491822-25491841 None:intergenic 40.0%
CTAACATCAAGACCCAAAAC+TGG + Chr6:25492756-25492775 None:intergenic 40.0%
GAAAAGGAGAAGAATAGGTC+TGG - Chr6:25490872-25490891 MsG0680031731.01.T01:CDS 40.0%
GAACTGAAAAGGAGAAGAAG+AGG - Chr6:25490894-25490913 MsG0680031731.01.T01:CDS 40.0%
GAACTGAAAAGGAGAAGAAG+TGG - Chr6:25490894-25490913 MsG0680031731.01.T01:CDS 40.0%
GATAAAGCTGGGATAGAGAA+AGG + Chr6:25491810-25491829 None:intergenic 40.0%
GCAAAAAGAAACCCAAGCAA+TGG + Chr6:25491226-25491245 None:intergenic 40.0%
GCAGATAATTCACATCACGA+GGG - Chr6:25493463-25493482 MsG0680031731.01.T01:CDS 40.0%
GGAATTTCACAAGGTTCTTC+TGG + Chr6:25493073-25493092 None:intergenic 40.0%
GGTAGAAGAGAGAGTTTAGA+TGG - Chr6:25493400-25493419 MsG0680031731.01.T01:intron 40.0%
GTATATACAATCTTCCACGG+TGG + Chr6:25491729-25491748 None:intergenic 40.0%
GTGAATTATCTGCAAGTGCT+TGG + Chr6:25493456-25493475 None:intergenic 40.0%
GTGAGAAGTTCTTGAAACCA+AGG - Chr6:25492149-25492168 MsG0680031731.01.T01:intron 40.0%
TAAAGGGGTGAAAGAGATAG+TGG - Chr6:25492463-25492482 MsG0680031731.01.T01:intron 40.0%
TAGACTGAGCCTGGTAATAA+AGG + Chr6:25491682-25491701 None:intergenic 40.0%
TCAAAAGACTACCGAGTTGT+TGG - Chr6:25492343-25492362 MsG0680031731.01.T01:intron 40.0%
TCCAAAAACAATTGAGCCGA+AGG + Chr6:25492616-25492635 None:intergenic 40.0%
TGCAGATAATTCACATCACG+AGG - Chr6:25493462-25493481 MsG0680031731.01.T01:CDS 40.0%
TGTGTTTACTAGCCAAATGG+AGG - Chr6:25492255-25492274 MsG0680031731.01.T01:intron 40.0%
TTAGACCTGGGAATTTCACA+AGG + Chr6:25493082-25493101 None:intergenic 40.0%
TTATTACCAGGCTCAGTCTA+GGG - Chr6:25491682-25491701 MsG0680031731.01.T01:intron 40.0%
TTCCCTTTCCCTTTATTACC+AGG - Chr6:25491670-25491689 MsG0680031731.01.T01:intron 40.0%
TTTATTACCAGGCTCAGTCT+AGG - Chr6:25491681-25491700 MsG0680031731.01.T01:intron 40.0%
! ATAGGTCTGGAACTGAAAAG+GGG - Chr6:25490885-25490904 MsG0680031731.01.T01:CDS 40.0%
! GAATAGGTCTGGAACTGAAA+AGG - Chr6:25490883-25490902 MsG0680031731.01.T01:CDS 40.0%
! TAATAAGGCACCTTCTAAGG+TGG + Chr6:25493507-25493526 None:intergenic 40.0%
! TGGTAATAAGGCACCTTCTA+AGG + Chr6:25493510-25493529 None:intergenic 40.0%
! TTCTTCTCCTTTTCAGTTCC+AGG + Chr6:25490866-25490885 None:intergenic 40.0%
! TTTATTGGCGATGGATTTGG+GGG - Chr6:25492501-25492520 MsG0680031731.01.T01:intron 40.0%
!! AAGGTTGGTGATTTGAAGGT+AGG + Chr6:25491900-25491919 None:intergenic 40.0%
!! AGGTTGGTGATTTGAAGGTA+GGG + Chr6:25491899-25491918 None:intergenic 40.0%
!! CAGTTTTAGTGCTTGAAGGT+TGG - Chr6:25492582-25492601 MsG0680031731.01.T01:intron 40.0%
!! TAGGAAGGTTGGTGATTTGA+AGG + Chr6:25491904-25491923 None:intergenic 40.0%
!!! AATTTTTAGTGCGCCTTGCA+CGG - Chr6:25492905-25492924 MsG0680031731.01.T01:intron 40.0%
!!! CGGTTGTTGTGGTGTTTTTT+GGG - Chr6:25492822-25492841 MsG0680031731.01.T01:intron 40.0%
!!! TAGAATTGCATTTTGGGGTG+CGG - Chr6:25492213-25492232 MsG0680031731.01.T01:intron 40.0%
!!! TCGGTTGTTGTGGTGTTTTT+TGG - Chr6:25492821-25492840 MsG0680031731.01.T01:intron 40.0%
AAACCCAAGCAATGGAGTTG+GGG + Chr6:25491218-25491237 None:intergenic 45.0%
AACCATCATCAACTCCGACA+TGG + Chr6:25493917-25493936 None:intergenic 45.0%
AGAAACCCAAGCAATGGAGT+TGG + Chr6:25491220-25491239 None:intergenic 45.0%
AGGAACCTCATTGACCATGT+CGG - Chr6:25493900-25493919 MsG0680031731.01.T01:CDS 45.0%
AGTTACCCATCCACCTTAGA+AGG - Chr6:25493494-25493513 MsG0680031731.01.T01:CDS 45.0%
ATCTGCAAGTGCTTGGTATG+CGG + Chr6:25493449-25493468 None:intergenic 45.0%
CAAAAGCCTAAGGACGACAA+AGG - Chr6:25491592-25491611 MsG0680031731.01.T01:intron 45.0%
CAGCAGCTACCTAAAAGGAA+AGG + Chr6:25492786-25492805 None:intergenic 45.0%
CCAAACATCATCCAACTTGG+TGG + Chr6:25493824-25493843 None:intergenic 45.0%
CCACCAAACATCATCCAACT+TGG + Chr6:25493827-25493846 None:intergenic 45.0%
CGAATGGAATTGGAACCTGA+TGG - Chr6:25492535-25492554 MsG0680031731.01.T01:intron 45.0%
CTGTGCACAGTCCGAAAATA+TGG + Chr6:25492654-25492673 None:intergenic 45.0%
GAAAAGGAGAAGAAGTGGTC+TGG - Chr6:25490899-25490918 MsG0680031731.01.T01:CDS 45.0%
GAAACCCAAGCAATGGAGTT+GGG + Chr6:25491219-25491238 None:intergenic 45.0%
GAAATCGTCGTATGTGAGGT+CGG - Chr6:25491181-25491200 MsG0680031731.01.T01:intron 45.0%
GAACTGAAAAGGGGAAGAAG+AGG - Chr6:25491110-25491129 MsG0680031731.01.T01:intron 45.0%
GAAGAGGTCTGCAACTGAAA+AGG - Chr6:25490964-25490983 MsG0680031731.01.T01:intron 45.0%
GAGCCTGGTAATAAAGGGAA+AGG + Chr6:25491676-25491695 None:intergenic 45.0%
GGAAGGTTTCTGTGAAGCAT+TGG - Chr6:25491295-25491314 MsG0680031731.01.T01:intron 45.0%
GTTTACTAGCCAAATGGAGG+CGG - Chr6:25492258-25492277 MsG0680031731.01.T01:intron 45.0%
TATTAACGACGAACCGTGCA+AGG + Chr6:25492921-25492940 None:intergenic 45.0%
TCACAAGGTTCTTCTGGTCA+TGG + Chr6:25493067-25493086 None:intergenic 45.0%
TCACGCATCTCCCAAAAAGA+CGG - Chr6:25491150-25491169 MsG0680031731.01.T01:intron 45.0%
TTTACTAGCCAAATGGAGGC+GGG - Chr6:25492259-25492278 MsG0680031731.01.T01:intron 45.0%
! ACGCGGCGATTTGGAAAATT+TGG - Chr6:25492405-25492424 MsG0680031731.01.T01:intron 45.0%
! AGCATTGGAACGAAGGGATT+CGG - Chr6:25491310-25491329 MsG0680031731.01.T01:intron 45.0%
! AGTGAGTTGCTGCCAGTTTT+GGG - Chr6:25492741-25492760 MsG0680031731.01.T01:intron 45.0%
! AGTTGGATGATGTTTGGTGG+TGG - Chr6:25493827-25493846 MsG0680031731.01.T01:CDS 45.0%
! ATCATCCAACTTGGTGGGAA+GGG + Chr6:25493818-25493837 None:intergenic 45.0%
! ATGGAATGCTTGTTGCGCTA+AGG - Chr6:25492554-25492573 MsG0680031731.01.T01:intron 45.0%
! CATATTTTCGGACTGTGCAC+AGG - Chr6:25492652-25492671 MsG0680031731.01.T01:intron 45.0%
! CCAAGTTGGATGATGTTTGG+TGG - Chr6:25493824-25493843 MsG0680031731.01.T01:CDS 45.0%
! CCACCAAGTTGGATGATGTT+TGG - Chr6:25493821-25493840 MsG0680031731.01.T01:CDS 45.0%
! GAAGTGGTCTGGAACTGAAA+AGG - Chr6:25490910-25490929 MsG0680031731.01.T01:CDS 45.0%
! GCCTTCGGCTCAATTGTTTT+TGG - Chr6:25492612-25492631 MsG0680031731.01.T01:intron 45.0%
! TAGGTGTGTCCTTCGGTTTT+TGG - Chr6:25493618-25493637 MsG0680031731.01.T01:intron 45.0%
! TCGTCGTTGACCGTCTTTTT+GGG + Chr6:25491163-25491182 None:intergenic 45.0%
! TGGAATGCTTGTTGCGCTAA+GGG - Chr6:25492555-25492574 MsG0680031731.01.T01:intron 45.0%
! TTATTGGCGATGGATTTGGG+GGG - Chr6:25492502-25492521 MsG0680031731.01.T01:intron 45.0%
! TTCGTCGTTGACCGTCTTTT+TGG + Chr6:25491164-25491183 None:intergenic 45.0%
!! CTGTGAAGCATTGGAACGAA+GGG - Chr6:25491304-25491323 MsG0680031731.01.T01:intron 45.0%
!! CTTAGAAGTTGTGATGGTGG+AGG - Chr6:25493379-25493398 MsG0680031731.01.T01:intron 45.0%
!! GGTTGGTGATTTGAAGGTAG+GGG + Chr6:25491898-25491917 None:intergenic 45.0%
!! TCTGTGAAGCATTGGAACGA+AGG - Chr6:25491303-25491322 MsG0680031731.01.T01:intron 45.0%
!! TGGGCAGTTTTAGTGCTTGA+AGG - Chr6:25492578-25492597 MsG0680031731.01.T01:intron 45.0%
!!! AGAATTGCATTTTGGGGTGC+GGG - Chr6:25492214-25492233 MsG0680031731.01.T01:intron 45.0%
!!! TTGCGGTGCTGCCATATTTT+CGG - Chr6:25492640-25492659 MsG0680031731.01.T01:intron 45.0%
AAACCCCCAACTCCATTGCT+TGG - Chr6:25491211-25491230 MsG0680031731.01.T01:intron 50.0%
AACCCAAGCAATGGAGTTGG+GGG + Chr6:25491217-25491236 None:intergenic 50.0%
AACCCCCAACTCCATTGCTT+GGG - Chr6:25491212-25491231 MsG0680031731.01.T01:intron 50.0%
ACAACCGATAGCACCCAAAC+TGG + Chr6:25492809-25492828 None:intergenic 50.0%
ACTACCCCTCACCAACAACT+CGG + Chr6:25492357-25492376 None:intergenic 50.0%
ACTGGCAGCAGCTACCTAAA+AGG + Chr6:25492791-25492810 None:intergenic 50.0%
AGACTACCGAGTTGTTGGTG+AGG - Chr6:25492348-25492367 MsG0680031731.01.T01:intron 50.0%
AGCGCAACAAGCATTCCATC+AGG + Chr6:25492553-25492572 None:intergenic 50.0%
AGGGGATAACGCTCTTTGGA+TGG - Chr6:25492293-25492312 MsG0680031731.01.T01:intron 50.0%
AGGTTCTTCTGGTCATGGCT+TGG + Chr6:25493062-25493081 None:intergenic 50.0%
CAACTCCGACATGGTCAATG+AGG + Chr6:25493908-25493927 None:intergenic 50.0%
CATGACAGGAGAATCGCAAC+AGG + Chr6:25492715-25492734 None:intergenic 50.0%
CGACGAAATCGTCGTATGTG+AGG - Chr6:25491177-25491196 MsG0680031731.01.T01:intron 50.0%
CTAGGGTGGATGAGCTTGAT+AGG - Chr6:25491699-25491718 MsG0680031731.01.T01:intron 50.0%
GGATAACGCTCTTTGGATGG+AGG - Chr6:25492296-25492315 MsG0680031731.01.T01:intron 50.0%
TAACGCAGCTTGGGACAAAG+AGG - Chr6:25491274-25491293 MsG0680031731.01.T01:intron 50.0%
TAGGTAGCTGCTGCCAGTTT+GGG - Chr6:25492793-25492812 MsG0680031731.01.T01:intron 50.0%
TCATTCCCTTCCCACCAAGT+TGG - Chr6:25493810-25493829 MsG0680031731.01.T01:CDS 50.0%
TGCCAAATCAACCTCAAGCG+CGG + Chr6:25492386-25492405 None:intergenic 50.0%
TGGAAGGGGATAACGCTCTT+TGG - Chr6:25492289-25492308 MsG0680031731.01.T01:intron 50.0%
TTACTAGCCAAATGGAGGCG+GGG - Chr6:25492260-25492279 MsG0680031731.01.T01:intron 50.0%
TTAGGTAGCTGCTGCCAGTT+TGG - Chr6:25492792-25492811 MsG0680031731.01.T01:intron 50.0%
! AATGCTTGTTGCGCTAAGGG+TGG - Chr6:25492558-25492577 MsG0680031731.01.T01:intron 50.0%
! ACTACCGAGTTGTTGGTGAG+GGG - Chr6:25492350-25492369 MsG0680031731.01.T01:intron 50.0%
! AGGGGTGAAAGAGATAGTGG+AGG - Chr6:25492466-25492485 MsG0680031731.01.T01:intron 50.0%
! ATGCTTGTTGCGCTAAGGGT+GGG - Chr6:25492559-25492578 MsG0680031731.01.T01:intron 50.0%
! CAATGGAGTTGGGGGTTTTG+AGG + Chr6:25491209-25491228 None:intergenic 50.0%
! CAGTGAGTTGCTGCCAGTTT+TGG - Chr6:25492740-25492759 MsG0680031731.01.T01:intron 50.0%
! CATCATCCAACTTGGTGGGA+AGG + Chr6:25493819-25493838 None:intergenic 50.0%
! GACTACCGAGTTGTTGGTGA+GGG - Chr6:25492349-25492368 MsG0680031731.01.T01:intron 50.0%
! TGTTGGTGAGGGGTAGTACT+TGG - Chr6:25492360-25492379 MsG0680031731.01.T01:intron 50.0%
! TTGGGTGCTATCGGTTGTTG+TGG - Chr6:25492811-25492830 MsG0680031731.01.T01:intron 50.0%
!! AAGGCACCTTCTAAGGTGGA+TGG + Chr6:25493503-25493522 None:intergenic 50.0%
!! AGGCACCTTCTAAGGTGGAT+GGG + Chr6:25493502-25493521 None:intergenic 50.0%
!! CTTGAGGTTGATTTGGCACG+CGG - Chr6:25492388-25492407 MsG0680031731.01.T01:intron 50.0%
!! GACCATGTCGGAGTTGATGA+TGG - Chr6:25493912-25493931 MsG0680031731.01.T01:CDS 50.0%
!! GGAGATGTATAAGGCGTTGG+TGG + Chr6:25491789-25491808 None:intergenic 50.0%
!! TCTTTGGATGGAGGTGTTGG+TGG - Chr6:25492305-25492324 MsG0680031731.01.T01:intron 50.0%
AAGGTTGGGCATGCTGCCTT+CGG - Chr6:25492597-25492616 MsG0680031731.01.T01:intron 55.0%
GCAGCTTGGGACAAAGAGGA+AGG - Chr6:25491278-25491297 MsG0680031731.01.T01:intron 55.0%
GCTTGATAGGTCTTCCACCG+TGG - Chr6:25491712-25491731 MsG0680031731.01.T01:intron 55.0%
GGGGGGAGGACAAATACGAA+TGG - Chr6:25492519-25492538 MsG0680031731.01.T01:intron 55.0%
TACTAGCCAAATGGAGGCGG+GGG - Chr6:25492261-25492280 MsG0680031731.01.T01:intron 55.0%
TTACCAGGCTCAGTCTAGGG+TGG - Chr6:25491685-25491704 MsG0680031731.01.T01:intron 55.0%
! CGCTCTTTGGATGGAGGTGT+TGG - Chr6:25492302-25492321 MsG0680031731.01.T01:intron 55.0%
! GCTGCCAGTTTGGGTGCTAT+CGG - Chr6:25492802-25492821 MsG0680031731.01.T01:intron 55.0%
! TGATTTGGCACGCGGCGATT+TGG - Chr6:25492396-25492415 MsG0680031731.01.T01:intron 55.0%
ACTAGCCAAATGGAGGCGGG+GGG - Chr6:25492262-25492281 MsG0680031731.01.T01:intron 60.0%
CATCCACCCTAGACTGAGCC+TGG + Chr6:25491691-25491710 None:intergenic 60.0%
CTGTGCACAGGTTCTGCTGC+TGG - Chr6:25492664-25492683 MsG0680031731.01.T01:intron 60.0%
GTAGTACTTGGCCGCGCTTG+AGG - Chr6:25492372-25492391 MsG0680031731.01.T01:intron 60.0%
TGGGGTGCGGGATGTCAAAG+TGG - Chr6:25492226-25492245 MsG0680031731.01.T01:intron 60.0%
! GGCCGCGCTTGAGGTTGATT+TGG - Chr6:25492381-25492400 MsG0680031731.01.T01:intron 60.0%
! TTGGCGATGGATTTGGGGGG+AGG - Chr6:25492505-25492524 MsG0680031731.01.T01:intron 60.0%
AAATGGAGGCGGGGGGTTGG+TGG - Chr6:25492269-25492288 MsG0680031731.01.T01:intron 65.0%
ACCAACCCCCCGCCTCCATT+TGG + Chr6:25492270-25492289 None:intergenic 65.0%
GCCAAATGGAGGCGGGGGGT+TGG - Chr6:25492266-25492285 MsG0680031731.01.T01:intron 70.0%
!! AGGCGGGGGGTTGGTGGAAG+GGG - Chr6:25492275-25492294 MsG0680031731.01.T01:intron 70.0%
!! GAGGCGGGGGGTTGGTGGAA+GGG - Chr6:25492274-25492293 MsG0680031731.01.T01:intron 70.0%
!! GGAGGCGGGGGGTTGGTGGA+AGG - Chr6:25492273-25492292 MsG0680031731.01.T01:intron 75.0%
Chromosome Type Strat End Strand Name
Chr6 gene 25490860 25493984 25490860 ID=MsG0680031731.01;Name=MsG0680031731.01
Chr6 mRNA 25490860 25493984 25490860 ID=MsG0680031731.01.T01;Parent=MsG0680031731.01;Name=MsG0680031731.01.T01;_AED=0.44;_eAED=0.67;_QI=0|0|0|1|1|1|3|0|175
Chr6 exon 25493646 25493984 25493646 ID=MsG0680031731.01.T01:exon:15666;Parent=MsG0680031731.01.T01
Chr6 exon 25493457 25493562 25493457 ID=MsG0680031731.01.T01:exon:15665;Parent=MsG0680031731.01.T01
Chr6 exon 25490860 25490942 25490860 ID=MsG0680031731.01.T01:exon:15664;Parent=MsG0680031731.01.T01
Chr6 CDS 25493646 25493984 25493646 ID=MsG0680031731.01.T01:cds;Parent=MsG0680031731.01.T01
Chr6 CDS 25493457 25493562 25493457 ID=MsG0680031731.01.T01:cds;Parent=MsG0680031731.01.T01
Chr6 CDS 25490860 25490942 25490860 ID=MsG0680031731.01.T01:cds;Parent=MsG0680031731.01.T01
Gene Sequence

>MsG0680031731.01.T01

ATGCCTGGAACTGAAAAGGAGAAGAATAGGTCTGGAACTGAAAAGGAGAAGAAGTGGTCTGGAACTGAAAAGGAGAAGAATAGGTCTGGAACTGAAAAGGAGAAGAAGAGGTCTGCAACTGAAAAGGAGAAGAATAGGTCTGGAACTGAAAAGGAGAAGAAGAGGTCTGCAACTGAAAAGGAGAAGAATAGGTCTGGAACTGAAAAGGAGAAGAAGAGGTCTGCAACTGAAAAGGAGAAGAATAGGTCTGGAACTGAAAAGGGGAAGAAGAGGAAAGAACTTACTGAAGCTCACGCATCTCCCAAAAAGACGGTCAACGACGAAATCGTCGTATGTGAGCTTGGGACAAAGAGGAAGGTTTCTGTGAAGCATTGGAACGAAGGGATTCGGATTGACATTCGCGAATATTACTTAAAAGATGGCAAGCAAAGACCTACCAAAAAAGGAACCTCATTGACCATGTCGGAGTTGATGATGGTTCGTAATCACATTGGAAAAATTGACAATGCACTCAATGAGGACTCTTAG
Protein sequence

>MsG0680031731.01.T01

MPGTEKEKNRSGTEKEKKWSGTEKEKNRSGTEKEKKRSATEKEKNRSGTEKEKKRSATEKEKNRSGTEKEKKRSATEKEKNRSGTEKGKKRKELTEAHASPKKTVNDEIVVCELGTKRKVSVKHWNEGIRIDIREYYLKDGKQRPTKKGTSLTMSELMMVRNHIGKIDNALNEDS*