AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035694.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035694.01.T01 MTR_6g087880 98.077 104 2 0 1 104 174 277 1.30e-68 208
MsG0680035694.01.T01 MTR_2g101870 55.140 107 47 1 1 106 176 282 1.06e-38 131
MsG0680035694.01.T01 MTR_8g106580 46.226 106 55 1 1 104 162 267 9.52e-28 103
MsG0680035694.01.T01 MTR_6g081930 45.283 106 56 1 1 104 160 265 2.71e-26 99.4
MsG0680035694.01.T01 MTR_0154s0010 42.991 107 57 2 1 104 1 106 6.49e-24 89.4
MsG0680035694.01.T01 MTR_4g075730 40.187 107 60 1 1 103 182 288 1.12e-23 92.8
MsG0680035694.01.T01 MTR_8g086610 37.500 104 61 1 1 100 145 248 2.52e-22 88.6
MsG0680035694.01.T01 MTR_3g037650 39.048 105 60 2 1 104 142 243 7.47e-15 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035694.01.T01 AT3G24605 48.571 105 53 1 1 104 166 270 1.94e-30 110
MsG0680035694.01.T01 AT3G24600 48.077 104 54 0 1 104 171 274 2.35e-30 110
MsG0680035694.01.T01 AT5G42860 38.095 126 56 1 1 104 164 289 3.03e-23 91.7
MsG0680035694.01.T01 AT4G35170 38.182 110 65 1 1 107 164 273 2.60e-21 86.3
MsG0680035694.01.T01 AT1G45688 36.220 127 58 1 1 104 185 311 1.53e-20 84.7

Find 17 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGATCTTACAGTTGCAAC+TGG 0.467915 6:+110863116 MsG0680035694.01.T01:CDS
GGAAGCAAGGTTCCATTGTA+TGG 0.470323 6:+110863857 MsG0680035694.01.T01:CDS
AAGTCGCGCGGAAATGTTGT+AGG 0.496383 6:+110863947 MsG0680035694.01.T01:CDS
GTACATTGACAGACACTGTT+CGG 0.501903 6:-110863832 None:intergenic
TGGTGCAGACGTAGCTAGCT+CGG 0.520146 6:+110863883 MsG0680035694.01.T01:CDS
CGGAAATGTTGTAGGCAAGT+TGG 0.532289 6:+110863955 MsG0680035694.01.T01:CDS
AAGGTTCCATTGTATGGCGC+TGG 0.535461 6:+110863863 MsG0680035694.01.T01:CDS
ATACATAAGATTCACCGACT+TGG 0.574509 6:-110863093 None:intergenic
TCTGCACCAGCGCCATACAA+TGG 0.600404 6:-110863869 None:intergenic
GTCTTTGAAGTTAAGTCGCG+CGG 0.601720 6:+110863935 MsG0680035694.01.T01:CDS
ATGTTGTAGGCAAGTTGGTG+AGG 0.621904 6:+110863960 MsG0680035694.01.T01:CDS
CATTTATGTCAGCTCCAAGT+CGG 0.623350 6:+110863079 MsG0680035694.01.T01:CDS
TGTCAATGTACAAGGAAGCA+AGG 0.623368 6:+110863844 MsG0680035694.01.T01:CDS
ACAGTGTCTGTCAATGTACA+AGG 0.628282 6:+110863836 MsG0680035694.01.T01:CDS
TCTTACAGTTGCAACTGGTG+AGG 0.650775 6:+110863121 MsG0680035694.01.T01:CDS
GTAGCTAGCTCGGTGAACAA+CGG 0.660567 6:+110863893 MsG0680035694.01.T01:CDS
TGAGCAAGAGACACGCTGTG+TGG 0.698027 6:-110863989 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATAACTTACAGATGTTT+AGG - Chr6:110863617-110863636 None:intergenic 20.0%
!!! TAATTAAAAACTAGTCTGTT+TGG + Chr6:110863212-110863231 MsG0680035694.01.T01:intron 20.0%
!!! TGAGGTAAGTATTCTAAAAT+CGG + Chr6:110863139-110863158 MsG0680035694.01.T01:intron 25.0%
AGTCAAAGTGAGTTCAAAAT+TGG + Chr6:110863691-110863710 MsG0680035694.01.T01:intron 30.0%
ATAAGCACTTGGAAATTGTT+TGG + Chr6:110863263-110863282 MsG0680035694.01.T01:intron 30.0%
GTTTCTAAATCAACAAGTAC+TGG - Chr6:110863165-110863184 None:intergenic 30.0%
TAAGCACTTGGAAATTGTTT+GGG + Chr6:110863264-110863283 MsG0680035694.01.T01:intron 30.0%
! TTGAACTCACTTTGACTTAT+GGG - Chr6:110863688-110863707 None:intergenic 30.0%
! TTTGAACTCACTTTGACTTA+TGG - Chr6:110863689-110863708 None:intergenic 30.0%
AAATGCCGAAAAAAGTTGCA+GGG - Chr6:110863064-110863083 None:intergenic 35.0%
ATGGAACTTGACAGTAAGAA+TGG - Chr6:110863770-110863789 None:intergenic 35.0%
CAATTTCCAAGTGCTTATGT+CGG - Chr6:110863261-110863280 None:intergenic 35.0%
TAAATGCCGAAAAAAGTTGC+AGG - Chr6:110863065-110863084 None:intergenic 35.0%
! ATACATAAGATTCACCGACT+TGG - Chr6:110863096-110863115 None:intergenic 35.0%
!! AGTTCAAAATTGGCCTTTGA+AGG + Chr6:110863701-110863720 MsG0680035694.01.T01:intron 35.0%
!! CTATTTTTCATAAGCTGTCC+TGG - Chr6:110863312-110863331 None:intergenic 35.0%
!!! CAGAGCTAGAACTTTTTTGA+TGG + Chr6:110863454-110863473 MsG0680035694.01.T01:intron 35.0%
!!! CATAACCCTGCAACTTTTTT+CGG + Chr6:110863056-110863075 MsG0680035694.01.T01:CDS 35.0%
ACAGTGTCTGTCAATGTACA+AGG + Chr6:110863836-110863855 MsG0680035694.01.T01:CDS 40.0%
CATTTAAGCCACTCCTTCAA+AGG - Chr6:110863717-110863736 None:intergenic 40.0%
CATTTATGTCAGCTCCAAGT+CGG + Chr6:110863079-110863098 MsG0680035694.01.T01:CDS 40.0%
GTACATTGACAGACACTGTT+CGG - Chr6:110863835-110863854 None:intergenic 40.0%
TATCTACCGACATAAGCACT+TGG + Chr6:110863252-110863271 MsG0680035694.01.T01:intron 40.0%
TCTGATCTTACAGTTGCAAC+TGG + Chr6:110863116-110863135 MsG0680035694.01.T01:CDS 40.0%
TGTCAATGTACAAGGAAGCA+AGG + Chr6:110863844-110863863 MsG0680035694.01.T01:CDS 40.0%
TTTCTTCAACTGCAGCAACA+TGG - Chr6:110863789-110863808 None:intergenic 40.0%
!! AAAATTGGCCTTTGAAGGAG+TGG + Chr6:110863706-110863725 MsG0680035694.01.T01:intron 40.0%
CGGAAATGTTGTAGGCAAGT+TGG + Chr6:110863955-110863974 MsG0680035694.01.T01:CDS 45.0%
CTTATGTCTGTAAGCTCACC+AGG + Chr6:110863291-110863310 MsG0680035694.01.T01:intron 45.0%
GGAAGCAAGGTTCCATTGTA+TGG + Chr6:110863857-110863876 MsG0680035694.01.T01:CDS 45.0%
TCTTACAGTTGCAACTGGTG+AGG + Chr6:110863121-110863140 MsG0680035694.01.T01:CDS 45.0%
!! ATGTTGTAGGCAAGTTGGTG+AGG + Chr6:110863960-110863979 MsG0680035694.01.T01:CDS 45.0%
!! GTCTTTGAAGTTAAGTCGCG+CGG + Chr6:110863935-110863954 MsG0680035694.01.T01:CDS 45.0%
AAGGTTCCATTGTATGGCGC+TGG + Chr6:110863863-110863882 MsG0680035694.01.T01:CDS 50.0%
AAGTCGCGCGGAAATGTTGT+AGG + Chr6:110863947-110863966 MsG0680035694.01.T01:CDS 50.0%
! GTAGCTAGCTCGGTGAACAA+CGG + Chr6:110863893-110863912 MsG0680035694.01.T01:CDS 50.0%
TCTGCACCAGCGCCATACAA+TGG - Chr6:110863872-110863891 None:intergenic 55.0%
TGAGCAAGAGACACGCTGTG+TGG - Chr6:110863992-110864011 None:intergenic 55.0%
TGGTGCAGACGTAGCTAGCT+CGG + Chr6:110863883-110863902 MsG0680035694.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 110863020 110864015 110863020 ID=MsG0680035694.01;Name=MsG0680035694.01
Chr6 mRNA 110863020 110864015 110863020 ID=MsG0680035694.01.T01;Parent=MsG0680035694.01;Name=MsG0680035694.01.T01;_AED=0.30;_eAED=0.31;_QI=0|0|0|1|0|0|2|0|112
Chr6 exon 110863020 110863142 110863020 ID=MsG0680035694.01.T01:exon:9002;Parent=MsG0680035694.01.T01
Chr6 exon 110863800 110864015 110863800 ID=MsG0680035694.01.T01:exon:9003;Parent=MsG0680035694.01.T01
Chr6 CDS 110863020 110863142 110863020 ID=MsG0680035694.01.T01:cds;Parent=MsG0680035694.01.T01
Chr6 CDS 110863800 110864015 110863800 ID=MsG0680035694.01.T01:cds;Parent=MsG0680035694.01.T01
Gene Sequence

>MsG0680035694.01.T01

ATGCTTACAGTGAATTGTTCAATGAGAATGTTAGTGCATAACCCTGCAACTTTTTTCGGCATTTATGTCAGCTCCAAGTCGGTGAATCTTATGTATTCTGATCTTACAGTTGCAACTGGTGAGTTGAAGAAATATCATCAACAGCGAAAGAGTCGCCGAACAGTGTCTGTCAATGTACAAGGAAGCAAGGTTCCATTGTATGGCGCTGGTGCAGACGTAGCTAGCTCGGTGAACAACGGTAAAATATCGTTAACGCTTGTCTTTGAAGTTAAGTCGCGCGGAAATGTTGTAGGCAAGTTGGTGAGGACTAAACCACACAGCGTGTCTCTTGCTCAATAG

Protein sequence

>MsG0680035694.01.T01

MLTVNCSMRMLVHNPATFFGIYVSSKSVNLMYSDLTVATGELKKYHQQRKSRRTVSVNVQGSKVPLYGAGADVASSVNNGKISLTLVFEVKSRGNVVGKLVRTKPHSVSLAQ*