AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029359.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029359.01.T01 MTR_5g084870 79.853 273 51 2 1 273 165 433 1.37e-157 446
MsG0580029359.01.T01 MTR_5g084850 79.715 281 50 4 1 280 165 439 3.56e-156 442
MsG0580029359.01.T01 MTR_8g079950 69.258 283 83 3 1 280 165 446 9.12e-133 384
MsG0580029359.01.T01 MTR_8g079970 70.073 274 78 2 1 271 165 437 3.38e-132 382
MsG0580029359.01.T01 MTR_5g084900 70.943 265 65 3 13 277 108 360 1.20e-128 370
MsG0580029359.01.T01 MTR_8g079980 53.430 277 117 5 1 271 166 436 2.72e-87 267
MsG0580029359.01.T01 MTR_2g007880 34.559 272 148 10 22 268 187 453 1.37e-34 130
MsG0580029359.01.T01 MTR_7g110540 42.581 155 52 3 65 219 93 210 2.15e-33 122
MsG0580029359.01.T01 MTR_3g086020 31.481 270 162 11 1 265 164 415 5.64e-33 125
MsG0580029359.01.T01 MTR_8g467580 29.885 261 160 8 26 269 186 440 1.97e-23 99.4
MsG0580029359.01.T01 MTR_8g467590 28.794 257 162 7 29 269 189 440 3.34e-23 98.6
MsG0580029359.01.T01 MTR_1g094790 29.915 234 137 8 55 268 218 444 6.45e-18 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029359.01.T01 AT1G24430 30.435 276 172 7 1 268 165 428 8.33e-33 125
MsG0580029359.01.T01 AT3G26040 35.102 245 144 8 42 278 205 442 2.10e-32 124
MsG0580029359.01.T01 AT5G23970 31.128 257 149 11 29 268 179 424 1.19e-26 108
MsG0580029359.01.T01 AT1G24420 31.907 257 147 8 28 268 188 432 1.30e-26 108
MsG0580029359.01.T01 AT1G24420 31.907 257 147 8 28 268 188 432 1.30e-26 108
MsG0580029359.01.T01 AT5G47950 29.502 261 170 7 12 268 172 422 1.94e-24 102
MsG0580029359.01.T01 AT3G30280 28.000 225 147 5 55 268 219 439 1.92e-23 99.4
MsG0580029359.01.T01 AT5G47980 31.250 256 158 9 28 268 187 439 4.16e-21 92.8
MsG0580029359.01.T01 AT4G15390 27.240 279 184 9 1 268 172 442 5.59e-21 92.4
MsG0580029359.01.T01 AT4G15390 27.240 279 184 9 1 268 172 442 5.59e-21 92.4
MsG0580029359.01.T01 AT4G15400 28.571 224 146 6 54 268 213 431 3.37e-19 87.0

Find 53 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTGTCTCTAAATAATTT+TGG 0.114602 5:+95937839 None:intergenic
TCTCTCTTTAACACCATTTC+TGG 0.146770 5:+95938199 None:intergenic
CTGATGTATGAAACGGATTT+TGG 0.163156 5:-95937716 MsG0580029359.01.T01:CDS
AAAACAATGTGGAGTTTAAA+AGG 0.191415 5:-95938177 MsG0580029359.01.T01:CDS
CCTGCATCAAGTAAACTAAA+TGG 0.242735 5:+95938259 None:intergenic
TAAGCATGCAGTTTCAATAA+TGG 0.276522 5:-95938053 MsG0580029359.01.T01:CDS
GGAAATGGAACTAGAGGATT+TGG 0.287550 5:-95937906 MsG0580029359.01.T01:CDS
AAGCATGCAGTTTCAATAAT+GGG 0.295268 5:-95938052 MsG0580029359.01.T01:CDS
TTTCATCTTCCGCTAGCTTT+TGG 0.298032 5:+95938299 None:intergenic
CTGCATCAAGTAAACTAAAT+GGG 0.363837 5:+95938260 None:intergenic
AACACCATTTCTGGGAAGAT+AGG 0.377854 5:+95938208 None:intergenic
TGAAATGATCAAGTCTCTTA+AGG 0.392506 5:-95938140 MsG0580029359.01.T01:CDS
TTTCAATTTCATGAATGATT+GGG 0.401465 5:-95938332 None:intergenic
TCAACATGTTGAACATTGTC+TGG 0.404597 5:+95937521 None:intergenic
GATGACAAATAAGAAGGAAA+TGG 0.408625 5:-95937921 MsG0580029359.01.T01:CDS
TCATGCAAAATGACGCGGTC+TGG 0.413097 5:+95938020 None:intergenic
GAACATGGAAGAGAATAATA+TGG 0.423310 5:-95937591 MsG0580029359.01.T01:CDS
CAAAATTATTTAGAGACAAA+GGG 0.428339 5:-95937838 MsG0580029359.01.T01:CDS
TTGGTTTGCAAAATAAAAGA+AGG 0.434706 5:-95937887 MsG0580029359.01.T01:CDS
CTCTCTTTAACACCATTTCT+GGG 0.439266 5:+95938200 None:intergenic
TTTGCCTATCTTCCCAGAAA+TGG 0.440943 5:-95938212 MsG0580029359.01.T01:CDS
AAACAATGTGGAGTTTAAAA+GGG 0.448288 5:-95938176 MsG0580029359.01.T01:CDS
CCAAAATTATTTAGAGACAA+AGG 0.455461 5:-95937839 MsG0580029359.01.T01:CDS
AAAGTGTAGGGAACATACTT+TGG 0.455506 5:-95937955 MsG0580029359.01.T01:CDS
AAATAAATTAATAATTCTGA+TGG 0.463091 5:-95937648 MsG0580029359.01.T01:CDS
CCATTTAGTTTACTTGATGC+AGG 0.471532 5:-95938259 MsG0580029359.01.T01:CDS
TGTGGATCTTCGCAGAAGAA+TGG 0.488711 5:-95937996 MsG0580029359.01.T01:CDS
TAGGTTTCTGATGTATGAAA+CGG 0.509458 5:-95937723 MsG0580029359.01.T01:CDS
TGAAGAACAACAAGAAGTAC+TGG 0.510593 5:+95937670 None:intergenic
GTCTATGATGACAAATAAGA+AGG 0.522890 5:-95937927 MsG0580029359.01.T01:CDS
AAGATAGGCAAATCCCTTTG+TGG 0.529715 5:+95938223 None:intergenic
TGGTTTGCAAAATAAAAGAA+GGG 0.546147 5:-95937886 MsG0580029359.01.T01:CDS
ATGGATACAAGAGATGGAGA+TGG 0.549171 5:-95937629 MsG0580029359.01.T01:CDS
GTGTACAAGTTGTAATTGCA+TGG 0.557884 5:-95938081 MsG0580029359.01.T01:CDS
GATCAAGTCTCTTAAGGCAA+TGG 0.558721 5:-95938134 MsG0580029359.01.T01:CDS
TAAGAAGGAAATGGAACTAG+AGG 0.567220 5:-95937912 MsG0580029359.01.T01:CDS
GAAGTACTGGTGACCCATGT+TGG 0.571663 5:+95937683 None:intergenic
TGGATACAAGAGATGGAGAT+GGG 0.578563 5:-95937628 MsG0580029359.01.T01:CDS
TTGGGTGGGGAAAACCAACA+TGG 0.579583 5:-95937697 MsG0580029359.01.T01:CDS
TACACATGTGGGAGAATGCA+TGG 0.581681 5:+95938099 None:intergenic
AATTACAACTTGTACACATG+TGG 0.586248 5:+95938087 None:intergenic
AGGAGTTGAGAGCTCAAGAA+CGG 0.587951 5:+95937489 None:intergenic
AACATTGTCTGGATTAAGAG+AGG 0.594892 5:+95937532 None:intergenic
TGCATCAAGTAAACTAAATG+GGG 0.595118 5:+95938261 None:intergenic
ATTCTGATGGATACAAGAGA+TGG 0.596490 5:-95937635 MsG0580029359.01.T01:CDS
AGCATGCAGTTTCAATAATG+GGG 0.599332 5:-95938051 MsG0580029359.01.T01:CDS
GATTGAAGCAATTGTGAACA+TGG 0.613709 5:-95937606 MsG0580029359.01.T01:CDS
ATTACAACTTGTACACATGT+GGG 0.632861 5:+95938088 None:intergenic
TGGGTGGGGAAAACCAACAT+GGG 0.633850 5:-95937696 MsG0580029359.01.T01:CDS
ATTATTTAGAGACAAAGGGA+AGG 0.636304 5:-95937834 MsG0580029359.01.T01:CDS
CACAATCATGCAAAATGACG+CGG 0.654154 5:+95938015 None:intergenic
GTTAAAGAGAGAAAACAATG+TGG 0.678799 5:-95938188 MsG0580029359.01.T01:CDS
CATTATCAACCAAAAGCTAG+CGG 0.679421 5:-95938308 MsG0580029359.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAATTAATAATTCTGA+TGG - Chr5:95938176-95938195 MsG0580029359.01.T01:CDS 10.0%
!!! AGTAAATAATATTTTATTCT+CGG + Chr5:95938067-95938086 None:intergenic 10.0%
!!! GTAAATAATATTTTATTCTC+GGG + Chr5:95938066-95938085 None:intergenic 15.0%
!!! TATTATTTACTTTTAGTAGT+TGG - Chr5:95938076-95938095 MsG0580029359.01.T01:CDS 15.0%
!! CAAAATTATTTAGAGACAAA+GGG - Chr5:95937986-95938005 MsG0580029359.01.T01:CDS 20.0%
! AAAACAATGTGGAGTTTAAA+AGG - Chr5:95937647-95937666 MsG0580029359.01.T01:CDS 25.0%
! AAACAATGTGGAGTTTAAAA+GGG - Chr5:95937648-95937667 MsG0580029359.01.T01:CDS 25.0%
! CCAAAATTATTTAGAGACAA+AGG - Chr5:95937985-95938004 MsG0580029359.01.T01:CDS 25.0%
!! CCTTTGTCTCTAAATAATTT+TGG + Chr5:95937988-95938007 None:intergenic 25.0%
!! TGGTTTGCAAAATAAAAGAA+GGG - Chr5:95937938-95937957 MsG0580029359.01.T01:CDS 25.0%
!!! TTGGTTTGCAAAATAAAAGA+AGG - Chr5:95937937-95937956 MsG0580029359.01.T01:CDS 25.0%
AAGCATGCAGTTTCAATAAT+GGG - Chr5:95937772-95937791 MsG0580029359.01.T01:CDS 30.0%
AATTACAACTTGTACACATG+TGG + Chr5:95937740-95937759 None:intergenic 30.0%
ATTACAACTTGTACACATGT+GGG + Chr5:95937739-95937758 None:intergenic 30.0%
ATTATTTAGAGACAAAGGGA+AGG - Chr5:95937990-95938009 MsG0580029359.01.T01:CDS 30.0%
CTGCATCAAGTAAACTAAAT+GGG + Chr5:95937567-95937586 None:intergenic 30.0%
GAACATGGAAGAGAATAATA+TGG - Chr5:95938233-95938252 MsG0580029359.01.T01:CDS 30.0%
GATGACAAATAAGAAGGAAA+TGG - Chr5:95937903-95937922 MsG0580029359.01.T01:CDS 30.0%
GTCTATGATGACAAATAAGA+AGG - Chr5:95937897-95937916 MsG0580029359.01.T01:CDS 30.0%
GTTAAAGAGAGAAAACAATG+TGG - Chr5:95937636-95937655 MsG0580029359.01.T01:CDS 30.0%
TAAGCATGCAGTTTCAATAA+TGG - Chr5:95937771-95937790 MsG0580029359.01.T01:CDS 30.0%
TAGGTTTCTGATGTATGAAA+CGG - Chr5:95938101-95938120 MsG0580029359.01.T01:CDS 30.0%
TGAAATGATCAAGTCTCTTA+AGG - Chr5:95937684-95937703 MsG0580029359.01.T01:CDS 30.0%
TGCATCAAGTAAACTAAATG+GGG + Chr5:95937566-95937585 None:intergenic 30.0%
! AGCAACAATTTTTCCACAAA+GGG - Chr5:95937588-95937607 MsG0580029359.01.T01:CDS 30.0%
!! AATATTTTATTCTCGGGTTG+AGG + Chr5:95938060-95938079 None:intergenic 30.0%
!! TGATGTATGAAACGGATTTT+GGG - Chr5:95938109-95938128 MsG0580029359.01.T01:CDS 30.0%
!! TTACTTTTAGTAGTTGGTGT+AGG - Chr5:95938082-95938101 MsG0580029359.01.T01:CDS 30.0%
AAAGTGTAGGGAACATACTT+TGG - Chr5:95937869-95937888 MsG0580029359.01.T01:CDS 35.0%
AACATTGTCTGGATTAAGAG+AGG + Chr5:95938295-95938314 None:intergenic 35.0%
AGCATGCAGTTTCAATAATG+GGG - Chr5:95937773-95937792 MsG0580029359.01.T01:CDS 35.0%
CATTATCAACCAAAAGCTAG+CGG - Chr5:95937516-95937535 MsG0580029359.01.T01:CDS 35.0%
CCTGCATCAAGTAAACTAAA+TGG + Chr5:95937568-95937587 None:intergenic 35.0%
CTCTCTTTAACACCATTTCT+GGG + Chr5:95937627-95937646 None:intergenic 35.0%
GATTGAAGCAATTGTGAACA+TGG - Chr5:95938218-95938237 MsG0580029359.01.T01:CDS 35.0%
GTGTACAAGTTGTAATTGCA+TGG - Chr5:95937743-95937762 MsG0580029359.01.T01:CDS 35.0%
TAAGAAGGAAATGGAACTAG+AGG - Chr5:95937912-95937931 MsG0580029359.01.T01:CDS 35.0%
TCAACATGTTGAACATTGTC+TGG + Chr5:95938306-95938325 None:intergenic 35.0%
TCTCTCTTTAACACCATTTC+TGG + Chr5:95937628-95937647 None:intergenic 35.0%
! ATTCTGATGGATACAAGAGA+TGG - Chr5:95938189-95938208 MsG0580029359.01.T01:CDS 35.0%
! CATTGCCTGAAAAAAGTGTA+GGG - Chr5:95937857-95937876 MsG0580029359.01.T01:CDS 35.0%
! CCATTTAGTTTACTTGATGC+AGG - Chr5:95937565-95937584 MsG0580029359.01.T01:CDS 35.0%
! GAGCAACAATTTTTCCACAA+AGG - Chr5:95937587-95937606 MsG0580029359.01.T01:CDS 35.0%
! TGAAGAACAACAAGAAGTAC+TGG + Chr5:95938157-95938176 None:intergenic 35.0%
!! CTGATGTATGAAACGGATTT+TGG - Chr5:95938108-95938127 MsG0580029359.01.T01:CDS 35.0%
!!! ATGTTCCCTACACTTTTTTC+AGG + Chr5:95937865-95937884 None:intergenic 35.0%
AAGATAGGCAAATCCCTTTG+TGG + Chr5:95937604-95937623 None:intergenic 40.0%
ATGGATACAAGAGATGGAGA+TGG - Chr5:95938195-95938214 MsG0580029359.01.T01:CDS 40.0%
CACAATCATGCAAAATGACG+CGG + Chr5:95937812-95937831 None:intergenic 40.0%
GATCAAGTCTCTTAAGGCAA+TGG - Chr5:95937690-95937709 MsG0580029359.01.T01:CDS 40.0%
GGAAATGGAACTAGAGGATT+TGG - Chr5:95937918-95937937 MsG0580029359.01.T01:CDS 40.0%
TGGATACAAGAGATGGAGAT+GGG - Chr5:95938196-95938215 MsG0580029359.01.T01:CDS 40.0%
TTTGCCTATCTTCCCAGAAA+TGG - Chr5:95937612-95937631 MsG0580029359.01.T01:CDS 40.0%
! AACACCATTTCTGGGAAGAT+AGG + Chr5:95937619-95937638 None:intergenic 40.0%
! CCATTGCCTGAAAAAAGTGT+AGG - Chr5:95937856-95937875 MsG0580029359.01.T01:CDS 40.0%
!! GTATGAAACGGATTTTGGGT+GGG - Chr5:95938113-95938132 MsG0580029359.01.T01:CDS 40.0%
!! TATGAAACGGATTTTGGGTG+GGG - Chr5:95938114-95938133 MsG0580029359.01.T01:CDS 40.0%
!! TGTATGAAACGGATTTTGGG+TGG - Chr5:95938112-95938131 MsG0580029359.01.T01:CDS 40.0%
!!! ACACTTTTTTCAGGCAATGG+AGG + Chr5:95937856-95937875 None:intergenic 40.0%
!!! CCTACACTTTTTTCAGGCAA+TGG + Chr5:95937859-95937878 None:intergenic 40.0%
!!! CGCTAGCTTTTGGTTGATAA+TGG + Chr5:95937518-95937537 None:intergenic 40.0%
!!! TTTCATCTTCCGCTAGCTTT+TGG + Chr5:95937528-95937547 None:intergenic 40.0%
TACACATGTGGGAGAATGCA+TGG + Chr5:95937728-95937747 None:intergenic 45.0%
TGTGGATCTTCGCAGAAGAA+TGG - Chr5:95937828-95937847 MsG0580029359.01.T01:CDS 45.0%
!! CGCGTCATTTTGCATGATTG+TGG - Chr5:95937810-95937829 MsG0580029359.01.T01:CDS 45.0%
TCATGCAAAATGACGCGGTC+TGG + Chr5:95937807-95937826 None:intergenic 50.0%
TGGGTGGGGAAAACCAACAT+GGG - Chr5:95938128-95938147 MsG0580029359.01.T01:CDS 50.0%
TTGGGTGGGGAAAACCAACA+TGG - Chr5:95938127-95938146 MsG0580029359.01.T01:CDS 50.0%
!! GAAGTACTGGTGACCCATGT+TGG + Chr5:95938144-95938163 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 95937502 95938344 95937502 ID=MsG0580029359.01;Name=MsG0580029359.01
Chr5 mRNA 95937502 95938344 95937502 ID=MsG0580029359.01.T01;Parent=MsG0580029359.01;Name=MsG0580029359.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|280
Chr5 exon 95937502 95938344 95937502 ID=MsG0580029359.01.T01:exon:36077;Parent=MsG0580029359.01.T01
Chr5 CDS 95937502 95938344 95937502 ID=MsG0580029359.01.T01:cds;Parent=MsG0580029359.01.T01
Gene Sequence

>MsG0580029359.01.T01

ATGAATGATTGGGCCATTATCAACCAAAAGCTAGCGGAAGATGAAAAAGATTTATTAGTATCCCCATTTAGTTTACTTGATGCAGGAGCAACAATTTTTCCACAAAGGGATTTGCCTATCTTCCCAGAAATGGTGTTAAAGAGAGAAAACAATGTGGAGTTTAAAAGGGTTGTTTTTCAACTTGAAATGATCAAGTCTCTTAAGGCAATGGTAACTTCTAGTTCCATGCATTCTCCCACATGTGTACAAGTTGTAATTGCATGGATTTATAAGCATGCAGTTTCAATAATGGGGTTAAATTTCCAGACCGCGTCATTTTGCATGATTGTGGATCTTCGCAGAAGAATGGTTCCTCCATTGCCTGAAAAAAGTGTAGGGAACATACTTTGGTTTTCGTCTATGATGACAAATAAGAAGGAAATGGAACTAGAGGATTTGGTTTGCAAAATAAAAGAAGGGTTATCTGAGTGTTGTAACGTTTATCCAAAATTATTTAGAGACAAAGGGAAGGATAATTTGTCAGTGATATCTGAGTGCTTGAAGCAAGTTACAATGCCTCAACCCGAGAATAAAATATTATTTACTTTTAGTAGTTGGTGTAGGTTTCTGATGTATGAAACGGATTTTGGGTGGGGAAAACCAACATGGGTCACCAGTACTTCTTGTTGTTCTTCAAATAAATTAATAATTCTGATGGATACAAGAGATGGAGATGGGATTGAAGCAATTGTGAACATGGAAGAGAATAATATGGCAAACATTGAACATGACTTTGAGCTTCTTCAATGTGCCTCTCTTAATCCAGACAATGTTCAACATGTTGATTGTGTGCTCCGTTCTTGA

Protein sequence

>MsG0580029359.01.T01

MNDWAIINQKLAEDEKDLLVSPFSLLDAGATIFPQRDLPIFPEMVLKRENNVEFKRVVFQLEMIKSLKAMVTSSSMHSPTCVQVVIAWIYKHAVSIMGLNFQTASFCMIVDLRRRMVPPLPEKSVGNILWFSSMMTNKKEMELEDLVCKIKEGLSECCNVYPKLFRDKGKDNLSVISECLKQVTMPQPENKILFTFSSWCRFLMYETDFGWGKPTWVTSTSCCSSNKLIILMDTRDGDGIEAIVNMEENNMANIEHDFELLQCASLNPDNVQHVDCVLRS*