Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036481.01.T01 | VDC86370.1 | 76.398 | 161 | 6 | 1 | 1 | 129 | 1 | 161 | 1.52E-75 | 235 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036481.01.T01 | Q96558 | 77.64 | 161 | 4 | 1 | 1 | 129 | 1 | 161 | 1.02E-75 | 236 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036481.01.T01 | A0A3P6AM70 | 76.398 | 161 | 6 | 1 | 1 | 129 | 1 | 161 | 7.25e-76 | 235 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048506.01 | MsG0780036481.01 | 0.817117 | 3.894663e-52 | 2.811365e-49 |
| MsG0080048507.01 | MsG0780036481.01 | 0.801198 | 1.008016e-48 | 4.784905e-46 |
| MsG0180000535.01 | MsG0780036481.01 | 0.821540 | 3.824409e-53 | 3.120230e-50 |
| MsG0180001029.01 | MsG0780036481.01 | 0.802408 | 5.691082e-49 | 2.785729e-46 |
| MsG0180003637.01 | MsG0780036481.01 | 0.830810 | 2.381682e-55 | 2.536943e-52 |
| MsG0180005906.01 | MsG0780036481.01 | 0.836482 | 9.118432e-57 | 1.150619e-53 |
| MsG0380013843.01 | MsG0780036481.01 | 0.827801 | 1.280157e-54 | 1.248849e-51 |
| MsG0480019663.01 | MsG0780036481.01 | 0.803340 | 3.652464e-49 | 1.830777e-46 |
| MsG0480021300.01 | MsG0780036481.01 | 0.812554 | 3.999575e-51 | 2.550830e-48 |
| MsG0380017901.01 | MsG0780036481.01 | 0.803506 | 3.374872e-49 | 1.698969e-46 |
| MsG0580029713.01 | MsG0780036481.01 | 0.839147 | 1.885197e-57 | 2.580076e-54 |
| MsG0680030296.01 | MsG0780036481.01 | 0.819687 | 1.019196e-52 | 7.897481e-50 |
| MsG0680030613.01 | MsG0780036481.01 | 0.817367 | 3.420513e-52 | 2.486174e-49 |
| MsG0280007851.01 | MsG0780036481.01 | 0.821884 | 3.184562e-53 | 2.623730e-50 |
| MsG0280009329.01 | MsG0780036481.01 | 0.828913 | 6.901905e-55 | 6.955162e-52 |
| MsG0780036480.01 | MsG0780036481.01 | 0.871694 | 5.621694e-67 | 2.288849e-63 |
| MsG0780036481.01 | MsG0880042554.01 | 0.800656 | 1.301236e-48 | 6.092511e-46 |
| MsG0780036481.01 | MsG0880043495.01 | 0.800241 | 1.580417e-48 | 7.322046e-46 |
| MsG0780036481.01 | MsG0880043882.01 | 0.800264 | 1.563882e-48 | 7.249805e-46 |
| MsG0780036481.01 | MsG0880045949.01 | 0.802695 | 4.966270e-49 | 2.448714e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036481.01.T01 | MTR_7g012950 | 78.261 | 161 | 3 | 1 | 1 | 129 | 1 | 161 | 4.03e-78 | 239 |
| MsG0780036481.01.T01 | MTR_5g043970 | 73.292 | 161 | 11 | 1 | 1 | 129 | 1 | 161 | 1.36e-72 | 225 |
| MsG0780036481.01.T01 | MTR_4g082990 | 68.944 | 161 | 18 | 1 | 1 | 129 | 1 | 161 | 4.19e-67 | 211 |
| MsG0780036481.01.T01 | MTR_4g109360 | 67.500 | 160 | 19 | 2 | 3 | 129 | 4 | 163 | 3.39e-65 | 206 |
| MsG0780036481.01.T01 | MTR_6g014890 | 50.427 | 117 | 46 | 4 | 12 | 127 | 16 | 121 | 3.89e-21 | 84.7 |
| MsG0780036481.01.T01 | MTR_6g014905 | 61.290 | 62 | 23 | 1 | 17 | 78 | 1 | 61 | 8.90e-21 | 80.5 |
| MsG0780036481.01.T01 | MTR_6g014530 | 44.144 | 111 | 32 | 2 | 14 | 110 | 8 | 102 | 1.06e-19 | 79.0 |
| MsG0780036481.01.T01 | MTR_6g014930 | 50.000 | 80 | 27 | 3 | 17 | 85 | 1 | 78 | 2.09e-14 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036481.01.T01 | AT5G15490 | 76.398 | 161 | 6 | 1 | 1 | 129 | 1 | 161 | 5.05e-76 | 234 |
| MsG0780036481.01.T01 | AT5G39320 | 75.155 | 161 | 8 | 1 | 1 | 129 | 1 | 161 | 1.53e-74 | 230 |
| MsG0780036481.01.T01 | AT3G29360 | 75.155 | 161 | 8 | 1 | 1 | 129 | 1 | 161 | 2.46e-74 | 229 |
| MsG0780036481.01.T01 | AT3G29360 | 75.155 | 161 | 8 | 1 | 1 | 129 | 1 | 161 | 2.46e-74 | 229 |
| MsG0780036481.01.T01 | AT1G26570 | 72.671 | 161 | 12 | 1 | 1 | 129 | 1 | 161 | 7.09e-73 | 226 |
Find 40 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGCTGCAGATTTGACTTAT+TGG | 0.286704 | 7:-8718594 | MsG0780036481.01.T01:CDS |
| AATCCGGACCGTGTTCTTAT+TGG | 0.290407 | 7:-8718383 | MsG0780036481.01.T01:CDS |
| GGCTGCAGATTTGACTTATT+GGG | 0.291078 | 7:-8718593 | MsG0780036481.01.T01:CDS |
| TGGAAGCTGGTCACTGTTCC+AGG | 0.324880 | 7:+8718767 | None:intergenic |
| ACTGTTCCAGGCTGCAATGC+GGG | 0.355570 | 7:+8718779 | None:intergenic |
| ATTCCGGGTTTGATAGAATC+TGG | 0.368775 | 7:+8718440 | None:intergenic |
| AGTTCCTCAGCAAGGAATTC+CGG | 0.395425 | 7:+8718424 | None:intergenic |
| AGTCAAATCTGCAGCCTTGC+CGG | 0.401486 | 7:+8718600 | None:intergenic |
| GTTCCTCAGCAAGGAATTCC+GGG | 0.420420 | 7:+8718425 | None:intergenic |
| AAGATTTGCTGCATTGGTGC+TGG | 0.447077 | 7:-8718882 | MsG0780036481.01.T01:CDS |
| TCAAGGCCAGGTTCATAGAT+TGG | 0.464203 | 7:+8718747 | None:intergenic |
| ACCGTGTTCTTATTGGAGGC+AGG | 0.464804 | 7:-8718376 | MsG0780036481.01.T01:CDS |
| AGTGCAATGACTGCCATTGT+TGG | 0.474498 | 7:+8718846 | None:intergenic |
| CCGGACCGTGTTCTTATTGG+AGG | 0.477174 | 7:-8718380 | MsG0780036481.01.T01:CDS |
| GTCAACAACAGCCACTTCAA+TGG | 0.518371 | 7:+8718812 | None:intergenic |
| AAAACTCAGGGTCTCGGTGC+CGG | 0.534565 | 7:-8718619 | MsG0780036481.01.T01:intron |
| ATGGTGAAGATTTGCTGCAT+TGG | 0.539016 | 7:-8718888 | MsG0780036481.01.T01:CDS |
| CTAAACCCCGCATTGCAGCC+TGG | 0.548047 | 7:-8718785 | MsG0780036481.01.T01:CDS |
| CAGCTTCCAATCTATGAACC+TGG | 0.551514 | 7:-8718753 | MsG0780036481.01.T01:CDS |
| TCAGGGTCTCGGTGCCGGCA+AGG | 0.556514 | 7:-8718614 | MsG0780036481.01.T01:intron |
| CCGTGTTCTTATTGGAGGCA+GGG | 0.558454 | 7:-8718375 | MsG0780036481.01.T01:CDS |
| TGCATTGGTGCTGGATATGT+CGG | 0.561538 | 7:-8718873 | MsG0780036481.01.T01:CDS |
| CTGTTCCAGGCTGCAATGCG+GGG | 0.564023 | 7:+8718780 | None:intergenic |
| CCTCCAATAAGAACACGGTC+CGG | 0.568819 | 7:+8718380 | None:intergenic |
| TGGAGGCAGGGAACCCCTGA+AGG | 0.570674 | 7:-8718363 | None:intergenic |
| CACTGTTCCAGGCTGCAATG+CGG | 0.572759 | 7:+8718778 | None:intergenic |
| TTTGTCAGACTTTGAGACAT+CGG | 0.579194 | 7:+8718549 | None:intergenic |
| ACAACAGCCACTTCAATGGA+TGG | 0.588532 | 7:+8718816 | None:intergenic |
| TTAACAACATCGTCAAGGCC+AGG | 0.594764 | 7:+8718735 | None:intergenic |
| CAGGTTCATAGATTGGAAGC+TGG | 0.598328 | 7:+8718754 | None:intergenic |
| TAAGTGTCCATCCATTGAAG+TGG | 0.605836 | 7:-8718823 | MsG0780036481.01.T01:CDS |
| CTGATTGCAGTTCCTCAGCA+AGG | 0.617902 | 7:+8718416 | None:intergenic |
| CCGACTAAAACTCAGGGTCT+CGG | 0.618298 | 7:-8718625 | MsG0780036481.01.T01:intron |
| ATTCCAGATTCTATCAAACC+CGG | 0.620754 | 7:-8718443 | MsG0780036481.01.T01:CDS |
| CCCTGCCTCCAATAAGAACA+CGG | 0.631372 | 7:+8718375 | None:intergenic |
| TGTCCCTGTCAAAACTGCTG+AGG | 0.636569 | 7:-8718506 | MsG0780036481.01.T01:CDS |
| ATATGTCGGTGGTCCAACAA+TGG | 0.658539 | 7:-8718859 | MsG0780036481.01.T01:CDS |
| CATGCTTAACAACATCGTCA+AGG | 0.675001 | 7:+8718730 | None:intergenic |
| AAACCCGGAATTCCTTGCTG+AGG | 0.680102 | 7:-8718428 | MsG0780036481.01.T01:CDS |
| ATTGGTGCTGGATATGTCGG+TGG | 0.703962 | 7:-8718870 | MsG0780036481.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCAGAGATCTTTTTAATC+CGG | - | Chr7:8718855-8718874 | MsG0780036481.01.T01:CDS | 25.0% |
| !!! | AATTTTGACTCACAATAGCA+AGG | - | Chr7:8718781-8718800 | MsG0780036481.01.T01:CDS | 30.0% |
| !!! | ATTTTGACTCACAATAGCAA+GGG | - | Chr7:8718782-8718801 | MsG0780036481.01.T01:CDS | 30.0% |
| ATTCCAGATTCTATCAAACC+CGG | - | Chr7:8718811-8718830 | MsG0780036481.01.T01:CDS | 35.0% | |
| ! | TGTTGAAAAGCATGTCTTTG+AGG | - | Chr7:8718577-8718596 | MsG0780036481.01.T01:CDS | 35.0% |
| !! | TTTGTCAGACTTTGAGACAT+CGG | + | Chr7:8718708-8718727 | None:intergenic | 35.0% |
| ATTCCGGGTTTGATAGAATC+TGG | + | Chr7:8718817-8718836 | None:intergenic | 40.0% | |
| CATGCTTAACAACATCGTCA+AGG | + | Chr7:8718527-8718546 | None:intergenic | 40.0% | |
| TAAGTGTCCATCCATTGAAG+TGG | - | Chr7:8718431-8718450 | MsG0780036481.01.T01:CDS | 40.0% | |
| ! | AGGCTGCAGATTTGACTTAT+TGG | - | Chr7:8718660-8718679 | MsG0780036481.01.T01:intron | 40.0% |
| ! | GGCTGCAGATTTGACTTATT+GGG | - | Chr7:8718661-8718680 | MsG0780036481.01.T01:intron | 40.0% |
| !! | ATGGTGAAGATTTGCTGCAT+TGG | - | Chr7:8718366-8718385 | MsG0780036481.01.T01:CDS | 40.0% |
| AACACCCCGACTAAAACTCA+GGG | - | Chr7:8718623-8718642 | MsG0780036481.01.T01:intron | 45.0% | |
| AATCCGGACCGTGTTCTTAT+TGG | - | Chr7:8718871-8718890 | MsG0780036481.01.T01:CDS | 45.0% | |
| ACAACAGCCACTTCAATGGA+TGG | + | Chr7:8718441-8718460 | None:intergenic | 45.0% | |
| AGTGCAATGACTGCCATTGT+TGG | + | Chr7:8718411-8718430 | None:intergenic | 45.0% | |
| AGTTCCTCAGCAAGGAATTC+CGG | + | Chr7:8718833-8718852 | None:intergenic | 45.0% | |
| ATATGTCGGTGGTCCAACAA+TGG | - | Chr7:8718395-8718414 | MsG0780036481.01.T01:CDS | 45.0% | |
| CAACATCTGTGCTGAAGAAG+AGG | + | Chr7:8718562-8718581 | None:intergenic | 45.0% | |
| CAGCTTCCAATCTATGAACC+TGG | - | Chr7:8718501-8718520 | MsG0780036481.01.T01:CDS | 45.0% | |
| CAGGTTCATAGATTGGAAGC+TGG | + | Chr7:8718503-8718522 | None:intergenic | 45.0% | |
| GTCAACAACAGCCACTTCAA+TGG | + | Chr7:8718445-8718464 | None:intergenic | 45.0% | |
| TAACACCCCGACTAAAACTC+AGG | - | Chr7:8718622-8718641 | MsG0780036481.01.T01:intron | 45.0% | |
| TCAAGGCCAGGTTCATAGAT+TGG | + | Chr7:8718510-8718529 | None:intergenic | 45.0% | |
| TGAAGAAGAGGTTCTTGCCA+CGG | + | Chr7:8718550-8718569 | None:intergenic | 45.0% | |
| TTAACAACATCGTCAAGGCC+AGG | + | Chr7:8718522-8718541 | None:intergenic | 45.0% | |
| !! | AAGATTTGCTGCATTGGTGC+TGG | - | Chr7:8718372-8718391 | MsG0780036481.01.T01:CDS | 45.0% |
| !! | ATTGCCTCAGCAGTTTTGAC+AGG | + | Chr7:8718755-8718774 | None:intergenic | 45.0% |
| !! | TGCATTGGTGCTGGATATGT+CGG | - | Chr7:8718381-8718400 | MsG0780036481.01.T01:CDS | 45.0% |
| !! | TTGCCTCAGCAGTTTTGACA+GGG | + | Chr7:8718754-8718773 | None:intergenic | 45.0% |
| AAACCCGGAATTCCTTGCTG+AGG | - | Chr7:8718826-8718845 | MsG0780036481.01.T01:CDS | 50.0% | |
| ACCGTGTTCTTATTGGAGGC+AGG | - | Chr7:8718878-8718897 | MsG0780036481.01.T01:CDS | 50.0% | |
| AGTCAAATCTGCAGCCTTGC+CGG | + | Chr7:8718657-8718676 | None:intergenic | 50.0% | |
| CCCTGCCTCCAATAAGAACA+CGG | + | Chr7:8718882-8718901 | None:intergenic | 50.0% | |
| CCGACTAAAACTCAGGGTCT+CGG | - | Chr7:8718629-8718648 | MsG0780036481.01.T01:intron | 50.0% | |
| CCGTGTTCTTATTGGAGGCA+GGG | - | Chr7:8718879-8718898 | MsG0780036481.01.T01:CDS | 50.0% | |
| CCTCCAATAAGAACACGGTC+CGG | + | Chr7:8718877-8718896 | None:intergenic | 50.0% | |
| CGATGTTGTTAAGCATGCCG+TGG | - | Chr7:8718530-8718549 | MsG0780036481.01.T01:CDS | 50.0% | |
| CTGATTGCAGTTCCTCAGCA+AGG | + | Chr7:8718841-8718860 | None:intergenic | 50.0% | |
| GTTCCTCAGCAAGGAATTCC+GGG | + | Chr7:8718832-8718851 | None:intergenic | 50.0% | |
| TGTCCCTGTCAAAACTGCTG+AGG | - | Chr7:8718748-8718767 | MsG0780036481.01.T01:CDS | 50.0% | |
| !! | ATTGGTGCTGGATATGTCGG+TGG | - | Chr7:8718384-8718403 | MsG0780036481.01.T01:CDS | 50.0% |
| !! | CCGAGACCCTGAGTTTTAGT+CGG | + | Chr7:8718632-8718651 | None:intergenic | 50.0% |
| !! | CGAGACCCTGAGTTTTAGTC+GGG | + | Chr7:8718631-8718650 | None:intergenic | 50.0% |
| !! | GAGACCCTGAGTTTTAGTCG+GGG | + | Chr7:8718630-8718649 | None:intergenic | 50.0% |
| ACTGTTCCAGGCTGCAATGC+GGG | + | Chr7:8718478-8718497 | None:intergenic | 55.0% | |
| CACTGTTCCAGGCTGCAATG+CGG | + | Chr7:8718479-8718498 | None:intergenic | 55.0% | |
| CCGGACCGTGTTCTTATTGG+AGG | - | Chr7:8718874-8718893 | MsG0780036481.01.T01:CDS | 55.0% | |
| TGGAAGCTGGTCACTGTTCC+AGG | + | Chr7:8718490-8718509 | None:intergenic | 55.0% | |
| ! | AAAACTCAGGGTCTCGGTGC+CGG | - | Chr7:8718635-8718654 | MsG0780036481.01.T01:intron | 55.0% |
| CTAAACCCCGCATTGCAGCC+TGG | - | Chr7:8718469-8718488 | MsG0780036481.01.T01:CDS | 60.0% | |
| CTGTTCCAGGCTGCAATGCG+GGG | + | Chr7:8718477-8718496 | None:intergenic | 60.0% | |
| !! | TCAGGGTCTCGGTGCCGGCA+AGG | - | Chr7:8718640-8718659 | MsG0780036481.01.T01:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 8718366 | 8718910 | 8718366 | ID=MsG0780036481.01;Name=MsG0780036481.01 |
| Chr7 | mRNA | 8718366 | 8718910 | 8718366 | ID=MsG0780036481.01.T01;Parent=MsG0780036481.01;Name=MsG0780036481.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|1|1|2|0|149 |
| Chr7 | exon | 8718728 | 8718910 | 8718728 | ID=MsG0780036481.01.T01:exon:1409;Parent=MsG0780036481.01.T01 |
| Chr7 | exon | 8718366 | 8718632 | 8718366 | ID=MsG0780036481.01.T01:exon:1408;Parent=MsG0780036481.01.T01 |
| Chr7 | CDS | 8718728 | 8718910 | 8718728 | ID=MsG0780036481.01.T01:cds;Parent=MsG0780036481.01.T01 |
| Chr7 | CDS | 8718366 | 8718632 | 8718366 | ID=MsG0780036481.01.T01:cds;Parent=MsG0780036481.01.T01 |
| Gene Sequence |
| Protein sequence |
