Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040168.01.T01 | XP_013449498.1 | 85.714 | 91 | 7 | 3 | 1 | 89 | 1 | 87 | 2.18E-44 | 148 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040168.01.T01 | A0A072U289 | 85.714 | 91 | 7 | 3 | 1 | 89 | 1 | 87 | 1.04e-44 | 148 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004203.01 | MsG0780040168.01 | 0.813721 | 2.217386e-51 | 1.459261e-48 |
| MsG0180004328.01 | MsG0780040168.01 | 0.845202 | 4.704567e-59 | 7.780678e-56 |
| MsG0180005648.01 | MsG0780040168.01 | 0.804464 | 2.133341e-49 | 1.100671e-46 |
| MsG0180005806.01 | MsG0780040168.01 | -0.805982 | 1.026103e-49 | 5.505297e-47 |
| MsG0280010802.01 | MsG0780040168.01 | 0.812182 | 4.821425e-51 | 3.044487e-48 |
| MsG0280010899.01 | MsG0780040168.01 | -0.803855 | 2.856694e-49 | 1.451055e-46 |
| MsG0480023921.01 | MsG0780040168.01 | 0.828939 | 6.801914e-55 | 6.859615e-52 |
| MsG0580024408.01 | MsG0780040168.01 | 0.811374 | 7.229644e-51 | 4.467633e-48 |
| MsG0380016086.01 | MsG0780040168.01 | 0.803864 | 2.844669e-49 | 1.445290e-46 |
| MsG0380016176.01 | MsG0780040168.01 | 0.808896 | 2.471870e-50 | 1.430384e-47 |
| MsG0380017859.01 | MsG0780040168.01 | 0.835566 | 1.557568e-56 | 1.911894e-53 |
| MsG0580030135.01 | MsG0780040168.01 | 0.818959 | 1.492546e-52 | 1.133562e-49 |
| MsG0280007418.01 | MsG0780040168.01 | -0.801180 | 1.016749e-48 | 4.824172e-46 |
| MsG0280008405.01 | MsG0780040168.01 | 0.806053 | 9.914177e-50 | 5.328678e-47 |
| MsG0780036661.01 | MsG0780040168.01 | 0.815432 | 9.272590e-52 | 6.392049e-49 |
| MsG0780040168.01 | MsG0780041177.01 | 0.810035 | 1.407867e-50 | 8.397756e-48 |
| MsG0780040168.01 | MsG0880047665.01 | 0.829721 | 4.393968e-55 | 4.534785e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040168.01.T01 | MTR_7g085610 | 85.714 | 91 | 7 | 3 | 1 | 89 | 1 | 87 | 2.64e-48 | 148 |
| MsG0780040168.01.T01 | MTR_6g029390 | 58.242 | 91 | 33 | 2 | 1 | 89 | 1 | 88 | 2.68e-31 | 105 |
| MsG0780040168.01.T01 | MTR_5g098200 | 57.303 | 89 | 29 | 3 | 1 | 85 | 1 | 84 | 8.63e-28 | 96.7 |
| MsG0780040168.01.T01 | MTR_5g098280 | 40.741 | 81 | 44 | 1 | 1 | 81 | 1 | 77 | 2.40e-14 | 62.4 |
| MsG0780040168.01.T01 | MTR_0398s0030 | 37.079 | 89 | 44 | 2 | 1 | 81 | 1 | 85 | 1.55e-12 | 57.8 |
| MsG0780040168.01.T01 | MTR_3g466420 | 42.683 | 82 | 42 | 2 | 1 | 77 | 1 | 82 | 2.25e-12 | 57.8 |
| MsG0780040168.01.T01 | MTR_5g075070 | 38.824 | 85 | 44 | 2 | 1 | 77 | 1 | 85 | 3.48e-12 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040168.01.T01 | AT5G18150 | 50.588 | 85 | 33 | 2 | 1 | 85 | 1 | 76 | 2.58e-24 | 87.4 |
| MsG0780040168.01.T01 | AT5G14602 | 47.059 | 85 | 40 | 2 | 1 | 85 | 1 | 80 | 6.64e-20 | 76.6 |
| MsG0780040168.01.T01 | AT5G58375 | 46.835 | 79 | 42 | 0 | 1 | 79 | 1 | 79 | 1.47e-19 | 75.9 |
Find 23 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGATTAAGAATGGATTT+TGG | 0.215303 | 7:-73897805 | MsG0780040168.01.T01:CDS |
| TCATCCAACAAGGATCATTT+TGG | 0.237826 | 7:-73897939 | MsG0780040168.01.T01:CDS |
| TGGCCAGTGGGAGGTATCTT+TGG | 0.321714 | 7:-73897769 | MsG0780040168.01.T01:CDS |
| TGGAAGAAATGTGTCCTCTT+AGG | 0.322863 | 7:-73898070 | None:intergenic |
| AAAACCAAAATGATCCTTGT+TGG | 0.442824 | 7:+73897935 | None:intergenic |
| TAGTTGGACATGATTATTTG+TGG | 0.484852 | 7:+73897733 | None:intergenic |
| TGGTTTCAGATGATTAAGAA+TGG | 0.487583 | 7:-73897812 | MsG0780040168.01.T01:intron |
| AAAATGATCCTTGTTGGATG+AGG | 0.493557 | 7:+73897941 | None:intergenic |
| GTCAAACTCCTGTGTGGTGG+AGG | 0.508255 | 7:+73897986 | None:intergenic |
| ACTCTTAAGAGTTCTGTAGT+TGG | 0.518236 | 7:+73897717 | None:intergenic |
| CTGTGTGGTGGAGGAGCTCA+CGG | 0.518529 | 7:+73897995 | None:intergenic |
| AAGATCAAGAGGAACCTAAG+AGG | 0.559496 | 7:+73898056 | None:intergenic |
| CGATAAATCCTCATCCAACA+AGG | 0.594591 | 7:-73897949 | MsG0780040168.01.T01:CDS |
| TTTCCAAAGATACCTCCCAC+TGG | 0.620026 | 7:+73897766 | None:intergenic |
| GTATTAGTTGACATGGCCAG+TGG | 0.636543 | 7:-73897782 | MsG0780040168.01.T01:CDS |
| TATTAGTTGACATGGCCAGT+GGG | 0.640261 | 7:-73897781 | MsG0780040168.01.T01:CDS |
| CGTGAGCTCCTCCACCACAC+AGG | 0.640612 | 7:-73897994 | MsG0780040168.01.T01:CDS |
| GGTGGTCCACGCAAAATAAG+CGG | 0.650418 | 7:+73898016 | None:intergenic |
| GAAGTCAAACTCCTGTGTGG+TGG | 0.665207 | 7:+73897983 | None:intergenic |
| TTGGTGATGAAAATAGACAA+AGG | 0.670898 | 7:+73897669 | None:intergenic |
| TAGTTGACATGGCCAGTGGG+AGG | 0.679321 | 7:-73897778 | MsG0780040168.01.T01:CDS |
| TGCGAAGTCAAACTCCTGTG+TGG | 0.690647 | 7:+73897980 | None:intergenic |
| TGTGGTGGAGGAGCTCACGG+TGG | 0.767016 | 7:+73897998 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGAATTGCATAAAGAAAATG+TGG | + | Chr7:73897800-73897819 | None:intergenic | 25.0% |
| !! | GAAATTTTTGTCACATACAT+TGG | + | Chr7:73898044-73898063 | None:intergenic | 25.0% |
| !!! | AGATGATTAAGAATGGATTT+TGG | - | Chr7:73897886-73897905 | MsG0780040168.01.T01:intron | 25.0% |
| !!! | ATTGACTTGAAATGTTACTT+TGG | - | Chr7:73897859-73897878 | MsG0780040168.01.T01:intron | 25.0% |
| !!! | GATGATTAAGAATGGATTTT+GGG | - | Chr7:73897887-73897906 | MsG0780040168.01.T01:intron | 25.0% |
| AAAACCAAAATGATCCTTGT+TGG | + | Chr7:73897759-73897778 | None:intergenic | 30.0% | |
| AATACAAGCACGAAAAAGAT+AGG | + | Chr7:73897842-73897861 | None:intergenic | 30.0% | |
| TAGTTGGACATGATTATTTG+TGG | + | Chr7:73897961-73897980 | None:intergenic | 30.0% | |
| TGGTTTCAGATGATTAAGAA+TGG | - | Chr7:73897879-73897898 | MsG0780040168.01.T01:intron | 30.0% | |
| ! | GGTATCTTTGGAAAAATTTG+AGG | - | Chr7:73897934-73897953 | MsG0780040168.01.T01:CDS | 30.0% |
| !! | ATTTTGGGTATTAGTTGACA+TGG | - | Chr7:73897902-73897921 | MsG0780040168.01.T01:intron | 30.0% |
| !! | TTGGTGATGAAAATAGACAA+AGG | + | Chr7:73898025-73898044 | None:intergenic | 30.0% |
| !!! | GGATCATTTTGGTTTTATCA+AGG | - | Chr7:73897763-73897782 | MsG0780040168.01.T01:CDS | 30.0% |
| AAAATGATCCTTGTTGGATG+AGG | + | Chr7:73897753-73897772 | None:intergenic | 35.0% | |
| ACTCTTAAGAGTTCTGTAGT+TGG | + | Chr7:73897977-73897996 | None:intergenic | 35.0% | |
| CATAAAGAAAATGTGGAACC+GGG | + | Chr7:73897793-73897812 | None:intergenic | 35.0% | |
| GCATAAAGAAAATGTGGAAC+CGG | + | Chr7:73897794-73897813 | None:intergenic | 35.0% | |
| ! | TCATCCAACAAGGATCATTT+TGG | - | Chr7:73897752-73897771 | MsG0780040168.01.T01:CDS | 35.0% |
| !!! | TGGTTTTATCAAGGTATTCC+CGG | - | Chr7:73897772-73897791 | MsG0780040168.01.T01:CDS | 35.0% |
| AAGATCAAGAGGAACCTAAG+AGG | + | Chr7:73897638-73897657 | None:intergenic | 40.0% | |
| CAGCTGAGAAAAAGATCAAG+AGG | + | Chr7:73897649-73897668 | None:intergenic | 40.0% | |
| CGATAAATCCTCATCCAACA+AGG | - | Chr7:73897742-73897761 | MsG0780040168.01.T01:CDS | 40.0% | |
| TATTAGTTGACATGGCCAGT+GGG | - | Chr7:73897910-73897929 | MsG0780040168.01.T01:intron | 40.0% | |
| ! | GATCTTTTTCTCAGCTGTGT+TGG | - | Chr7:73897652-73897671 | MsG0780040168.01.T01:three_prime_UTR | 40.0% |
| GTATTAGTTGACATGGCCAG+TGG | - | Chr7:73897909-73897928 | MsG0780040168.01.T01:intron | 45.0% | |
| TTTCCAAAGATACCTCCCAC+TGG | + | Chr7:73897928-73897947 | None:intergenic | 45.0% | |
| GAAGTCAAACTCCTGTGTGG+TGG | + | Chr7:73897711-73897730 | None:intergenic | 50.0% | |
| GGTGGTCCACGCAAAATAAG+CGG | + | Chr7:73897678-73897697 | None:intergenic | 50.0% | |
| TGCGAAGTCAAACTCCTGTG+TGG | + | Chr7:73897714-73897733 | None:intergenic | 50.0% | |
| !!! | TGTTGGCCGCTTATTTTGCG+TGG | - | Chr7:73897669-73897688 | MsG0780040168.01.T01:three_prime_UTR | 50.0% |
| GTCAAACTCCTGTGTGGTGG+AGG | + | Chr7:73897708-73897727 | None:intergenic | 55.0% | |
| TAGTTGACATGGCCAGTGGG+AGG | - | Chr7:73897913-73897932 | MsG0780040168.01.T01:intron | 55.0% | |
| !! | TGGCCAGTGGGAGGTATCTT+TGG | - | Chr7:73897922-73897941 | MsG0780040168.01.T01:CDS | 55.0% |
| ! | CTGTGTGGTGGAGGAGCTCA+CGG | + | Chr7:73897699-73897718 | None:intergenic | 60.0% |
| CGTGAGCTCCTCCACCACAC+AGG | - | Chr7:73897697-73897716 | MsG0780040168.01.T01:three_prime_UTR | 65.0% | |
| ! | TGTGGTGGAGGAGCTCACGG+TGG | + | Chr7:73897696-73897715 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 73897629 | 73898084 | 73897629 | ID=MsG0780040168.01;Name=MsG0780040168.01 |
| Chr7 | mRNA | 73897629 | 73898084 | 73897629 | ID=MsG0780040168.01.T01;Parent=MsG0780040168.01;Name=MsG0780040168.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0.5|1|0|0|2|92|89 |
| Chr7 | exon | 73897629 | 73897825 | 73897629 | ID=MsG0780040168.01.T01:exon:35177;Parent=MsG0780040168.01.T01 |
| Chr7 | exon | 73897920 | 73898084 | 73897920 | ID=MsG0780040168.01.T01:exon:35176;Parent=MsG0780040168.01.T01 |
| Chr7 | CDS | 73897920 | 73898084 | 73897920 | ID=MsG0780040168.01.T01:cds;Parent=MsG0780040168.01.T01 |
| Chr7 | CDS | 73897721 | 73897825 | 73897721 | ID=MsG0780040168.01.T01:cds;Parent=MsG0780040168.01.T01 |
| Chr7 | three_prime_UTR | 73897629 | 73897720 | 73897629 | ID=MsG0780040168.01.T01:three_prime_utr;Parent=MsG0780040168.01.T01 |
| Gene Sequence |
| Protein sequence |
