AlfalfaGEDB | MsG0780040612.01

M. sativa cultivar ZhongmuNo.1 / MsG0780040612.01

KEGG: -
GO: -
PFAM: PF03018

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040612.01.T01	XP_003625315.1	99.359	156	1	0	1	156	39	194	7.89E-111	323

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040612.01.T01	A0A1V1FH01	91.667	156	13	0	1	156	33	188	3.27E-106	303

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040612.01.T01	G7KWC3	99.359	156	1	0	1	156	39	194	3.77e-111	323

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0780040612.01	MsG0780040613.01	0.860299	2.275060e-63	6.188970e-60

PPI

Gene1	Gene2	Type
MsG0780040612.01	MsG0780040613.01	PPI
MsG0780040614.01	MsG0780040612.01	PPI
MsG0480021436.01	MsG0780040612.01	PPI
MsG0780040612.01	MsG0480018512.01	PPI
MsG0780040611.01	MsG0780040612.01	PPI
MsG0780040612.01	MsG0780040610.01	PPI
MsG0080048537.01	MsG0780040612.01	PPI
MsG0080048537.01	MsG0780040612.01	PPI
MsG0780040615.01	MsG0780040612.01	PPI
MsG0780040609.01	MsG0780040612.01	PPI
MsG0080048537.01	MsG0780040612.01	PPI
MsG0780040612.01	MsG0080048838.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040612.01.T01	MTR_7g093820	99.359	156	1	0	1	156	39	194	9.55e-115	323
MsG0780040612.01.T01	MTR_7g093850	96.795	156	5	0	1	156	39	194	5.47e-113	318
MsG0780040612.01.T01	MTR_7g093830	96.154	156	6	0	1	156	38	193	7.60e-112	315
MsG0780040612.01.T01	MTR_7g093790	81.410	156	29	0	1	156	36	191	3.34e-95	273
MsG0780040612.01.T01	MTR_7g093870	60.000	160	58	3	2	156	35	193	4.07e-65	198
MsG0780040612.01.T01	MTR_4g013350	53.503	157	71	2	1	156	42	197	3.30e-57	177
MsG0780040612.01.T01	MTR_4g013345	53.503	157	71	2	1	156	41	196	6.16e-56	174
MsG0780040612.01.T01	MTR_1g054525	55.263	152	65	2	5	156	44	192	2.99e-54	169
MsG0780040612.01.T01	MTR_4g013335	50.955	157	73	3	1	156	43	196	7.25e-54	169
MsG0780040612.01.T01	MTR_4g013320	51.974	152	72	1	5	156	49	199	1.19e-53	168
MsG0780040612.01.T01	MTR_4g013330	50.943	159	74	3	1	156	42	199	2.14e-53	167
MsG0780040612.01.T01	MTR_4g013325	53.425	146	67	1	11	156	55	199	7.60e-53	166
MsG0780040612.01.T01	MTR_4g073950	53.595	153	69	2	5	156	41	192	2.15e-51	162
MsG0780040612.01.T01	MTR_4g013310	51.974	152	70	2	5	156	49	197	2.72e-50	160
MsG0780040612.01.T01	MTR_3g105640	52.288	153	70	3	6	156	37	188	1.81e-49	157
MsG0780040612.01.T01	MTR_3g105630	50.000	154	73	3	6	156	37	189	6.34e-49	156
MsG0780040612.01.T01	MTR_7g070390	51.316	152	72	2	5	156	43	192	7.51e-47	151
MsG0780040612.01.T01	MTR_4g013385	45.395	152	81	2	5	156	41	190	2.14e-44	144
MsG0780040612.01.T01	MTR_0433s0040	45.395	152	81	2	5	156	41	190	2.14e-44	144
MsG0780040612.01.T01	MTR_5g096120	49.677	155	71	4	7	156	37	189	4.23e-44	144
MsG0780040612.01.T01	MTR_1g056370	46.939	147	75	3	10	155	41	185	4.45e-42	139
MsG0780040612.01.T01	MTR_4g013770	43.243	148	82	2	10	156	45	191	1.44e-41	137
MsG0780040612.01.T01	MTR_3g034030	45.033	151	78	4	7	156	39	185	2.74e-39	131
MsG0780040612.01.T01	MTR_4g078885	44.079	152	80	4	6	156	38	185	1.02e-38	130
MsG0780040612.01.T01	MTR_4g013355	40.789	152	79	1	5	156	39	179	3.60e-36	123
MsG0780040612.01.T01	MTR_4g122110	40.397	151	89	1	6	156	31	180	6.47e-30	107
MsG0780040612.01.T01	MTR_4g122130	40.397	151	89	1	6	156	31	180	1.73e-29	106
MsG0780040612.01.T01	MTR_4g049570	31.544	149	101	1	3	151	26	173	4.67e-21	84.7
MsG0780040612.01.T01	MTR_4g049550	31.507	146	99	1	6	151	29	173	1.20e-20	83.6
MsG0780040612.01.T01	MTR_1g046490	38.947	95	56	2	40	134	119	211	9.05e-14	66.6
MsG0780040612.01.T01	MTR_1g046800	40.000	95	55	2	40	134	133	225	9.41e-14	66.6
MsG0780040612.01.T01	MTR_1g046500	40.000	95	55	2	40	134	133	225	1.30e-13	66.2
MsG0780040612.01.T01	MTR_4g062520	37.383	107	66	1	33	139	177	282	1.08e-12	64.3
MsG0780040612.01.T01	MTR_1g115510	35.385	130	76	5	33	155	225	353	1.16e-12	64.7
MsG0780040612.01.T01	MTR_4g062470	37.383	107	66	1	33	139	178	283	1.37e-12	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040612.01.T01	AT5G42500	53.595	153	68	2	3	155	36	185	1.24e-53	168
MsG0780040612.01.T01	AT5G42510	51.634	153	71	2	3	155	33	182	4.43e-51	161
MsG0780040612.01.T01	AT1G65870	52.288	153	72	1	3	155	38	189	4.86e-51	161
MsG0780040612.01.T01	AT1G22900	51.634	153	71	2	3	155	44	193	1.88e-49	157
MsG0780040612.01.T01	AT1G58170	46.452	155	82	1	2	156	32	185	3.52e-48	154
MsG0780040612.01.T01	AT5G49040	46.053	152	81	1	5	156	41	191	1.63e-47	152
MsG0780040612.01.T01	AT1G55210	46.497	157	79	3	1	156	35	187	2.09e-47	152
MsG0780040612.01.T01	AT1G55210	46.497	157	79	3	1	156	35	187	2.09e-47	152
MsG0780040612.01.T01	AT3G13650	45.455	154	80	2	3	156	37	186	4.15e-46	149
MsG0780040612.01.T01	AT2G21100	48.667	150	76	1	6	155	39	187	1.35e-43	142
MsG0780040612.01.T01	AT2G21100	48.667	150	76	1	6	155	39	187	1.35e-43	142
MsG0780040612.01.T01	AT3G13662	47.857	140	69	2	4	143	37	172	1.32e-42	139
MsG0780040612.01.T01	AT2G21110	44.371	151	79	2	10	156	37	186	4.76e-42	139
MsG0780040612.01.T01	AT4G38700	43.671	158	84	3	4	156	33	190	1.07e-39	132
MsG0780040612.01.T01	AT5G49030	47.692	130	67	1	5	134	41	169	1.15e-37	136
MsG0780040612.01.T01	AT3G58090	46.269	134	71	1	22	155	139	271	2.07e-34	121
MsG0780040612.01.T01	AT3G13660	44.538	119	65	1	38	156	8	125	1.49e-33	115
MsG0780040612.01.T01	AT4G23690	34.586	133	75	5	5	131	36	162	1.44e-13	65.5
MsG0780040612.01.T01	AT3G24020	35.789	95	59	2	40	134	126	218	7.11e-11	58.9

Find 43 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGAAGTACACTTAGCATAT+TGG	0.334562	7:-79986782	MsG0780040612.01.T01:CDS
AAACTACACCTAGCTAATAA+TGG	0.351724	7:+79986605	None:intergenic
ACAATGGCCATTTCTCTAAC+AGG	0.363166	7:+79986736	None:intergenic
ACAAATACCATGCTAGGTTT+TGG	0.370876	7:+79987000	None:intergenic
GGAAGTACACTTAGCATATT+GGG	0.380807	7:-79986781	MsG0780040612.01.T01:CDS
TTCCACAATGGCATCTCCTT+TGG	0.400130	7:+79986645	None:intergenic
ATGGCCATTGTTGGTGGGAC+TGG	0.413384	7:-79986724	MsG0780040612.01.T01:CDS
CGTTCAGGACCAGCCGTCAA+TGG	0.424944	7:+79986913	None:intergenic
TACTTTCACGACATTGTGTC+AGG	0.427188	7:-79987024	MsG0780040612.01.T01:CDS
GAGTGAACCTGTTAGAGAAA+TGG	0.463422	7:-79986743	MsG0780040612.01.T01:CDS
TTCTATATCACAAGAGGAGA+TGG	0.477985	7:-79986851	MsG0780040612.01.T01:CDS
TTCAGTGATGGAGATTTCAA+TGG	0.482811	7:-79986802	MsG0780040612.01.T01:CDS
ACAAGTTTCGAGTCACGTTC+AGG	0.491447	7:+79986898	None:intergenic
ATTCGGGACGGTTGTGGCGA+TGG	0.502476	7:-79986941	MsG0780040612.01.T01:CDS
GTTGCCATTCGGGACGGTTG+TGG	0.502902	7:-79986947	MsG0780040612.01.T01:CDS
GTTCAGGACCAGCCGTCAAT+GGG	0.519940	7:+79986914	None:intergenic
GGCGATGGATGACCCATTGA+CGG	0.521036	7:-79986926	MsG0780040612.01.T01:CDS
GTATTTGTAGCTGAGCCTAA+TGG	0.523113	7:-79986985	MsG0780040612.01.T01:CDS
GAACGTGACTCGAAACTTGT+GGG	0.527774	7:-79986895	MsG0780040612.01.T01:CDS
AGCCTGTCCCCAACAATGCT+GGG	0.528229	7:-79987081	None:intergenic
AGAGGAGATGGGACTAATGA+TGG	0.528699	7:-79986839	MsG0780040612.01.T01:CDS
TATTCTGTTGATTATACCAA+AGG	0.532705	7:-79986661	MsG0780040612.01.T01:CDS
TGACTCGAAACTTGTGGGTA+AGG	0.534298	7:-79986890	MsG0780040612.01.T01:CDS
ATGGATGACCCATTGACGGC+TGG	0.541350	7:-79986922	MsG0780040612.01.T01:CDS
GTTAGAGAAATGGCCATTGT+TGG	0.545345	7:-79986733	MsG0780040612.01.T01:CDS
AAACTTGTGGGTAAGGCTCA+AGG	0.545821	7:-79986883	MsG0780040612.01.T01:CDS
ATGACCATGGCGTTCAGTGA+TGG	0.546366	7:-79986814	MsG0780040612.01.T01:CDS
TCAGCTACAAATACCATGCT+AGG	0.557255	7:+79986994	None:intergenic
TTACACTTCTATATCACAAG+AGG	0.558364	7:-79986857	MsG0780040612.01.T01:CDS
AAATGCGTTGCCATTCGGGA+CGG	0.561926	7:-79986953	MsG0780040612.01.T01:CDS
AGAGAAATGGCCATTGTTGG+TGG	0.569066	7:-79986730	MsG0780040612.01.T01:CDS
AGCTCCAGTCCCACCAACAA+TGG	0.574074	7:+79986720	None:intergenic
TGAACGTGACTCGAAACTTG+TGG	0.588799	7:-79986896	MsG0780040612.01.T01:CDS
ATCGCCACAACCGTCCCGAA+TGG	0.592145	7:+79986943	None:intergenic
TCTATATCACAAGAGGAGAT+GGG	0.592301	7:-79986850	MsG0780040612.01.T01:CDS
GAGAAATGGCCATTGTTGGT+GGG	0.599344	7:-79986729	MsG0780040612.01.T01:CDS
ATCTCCATCACTGAACGCCA+TGG	0.609829	7:+79986810	None:intergenic
GTCAGGACCAAAACCTAGCA+TGG	0.622028	7:-79987007	MsG0780040612.01.T01:CDS
TGTAGCTGAGCCTAATGGCA+AGG	0.630219	7:-79986980	MsG0780040612.01.T01:CDS
TACCAAAGGAGATGCCATTG+TGG	0.635951	7:-79986647	MsG0780040612.01.T01:CDS
TGTCGTGAAAGTAAAAGTGA+AGG	0.637192	7:+79987034	None:intergenic
GAATACATCATATTCCACAA+TGG	0.695532	7:+79986633	None:intergenic
ACTAATGATGGTGATGACCA+TGG	0.699161	7:-79986827	MsG0780040612.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATTCTGTTGATTATACCAA+AGG	-	Chr7:79987023-79987042	MsG0780040612.01.T01:CDS	25.0%
	GAATACATCATATTCCACAA+TGG	+	Chr7:79987054-79987073	None:intergenic	30.0%
	TTACACTTCTATATCACAAG+AGG	-	Chr7:79986827-79986846	MsG0780040612.01.T01:CDS	30.0%
	GTAAAAAATGCGTTGCCATT+CGG	-	Chr7:79986726-79986745	MsG0780040612.01.T01:CDS	35.0%
	TAAAAAATGCGTTGCCATTC+GGG	-	Chr7:79986727-79986746	MsG0780040612.01.T01:CDS	35.0%
	TCTATATCACAAGAGGAGAT+GGG	-	Chr7:79986834-79986853	MsG0780040612.01.T01:CDS	35.0%
	TGTCGTGAAAGTAAAAGTGA+AGG	+	Chr7:79986653-79986672	None:intergenic	35.0%
	TTCTATATCACAAGAGGAGA+TGG	-	Chr7:79986833-79986852	MsG0780040612.01.T01:CDS	35.0%
!	ACAAATACCATGCTAGGTTT+TGG	+	Chr7:79986687-79986706	None:intergenic	35.0%
!	GGAAGTACACTTAGCATATT+GGG	-	Chr7:79986903-79986922	MsG0780040612.01.T01:CDS	35.0%
!	TGGAAGTACACTTAGCATAT+TGG	-	Chr7:79986902-79986921	MsG0780040612.01.T01:CDS	35.0%
!	TTCAGTGATGGAGATTTCAA+TGG	-	Chr7:79986882-79986901	MsG0780040612.01.T01:CDS	35.0%
!!!	GCATTTTTTACCTTGCCATT+AGG	+	Chr7:79986717-79986736	None:intergenic	35.0%
	ACAATGGCCATTTCTCTAAC+AGG	+	Chr7:79986951-79986970	None:intergenic	40.0%
	GAGTGAACCTGTTAGAGAAA+TGG	-	Chr7:79986941-79986960	MsG0780040612.01.T01:CDS	40.0%
	GTATTTGTAGCTGAGCCTAA+TGG	-	Chr7:79986699-79986718	MsG0780040612.01.T01:CDS	40.0%
	GTTAGAGAAATGGCCATTGT+TGG	-	Chr7:79986951-79986970	MsG0780040612.01.T01:CDS	40.0%
	TACTTTCACGACATTGTGTC+AGG	-	Chr7:79986660-79986679	MsG0780040612.01.T01:CDS	40.0%
	TCAGCTACAAATACCATGCT+AGG	+	Chr7:79986693-79986712	None:intergenic	40.0%
!	ACTAATGATGGTGATGACCA+TGG	-	Chr7:79986857-79986876	MsG0780040612.01.T01:CDS	40.0%
	AAACTTGTGGGTAAGGCTCA+AGG	-	Chr7:79986801-79986820	MsG0780040612.01.T01:CDS	45.0%
	AGAGAAATGGCCATTGTTGG+TGG	-	Chr7:79986954-79986973	MsG0780040612.01.T01:CDS	45.0%
	AGAGGAGATGGGACTAATGA+TGG	-	Chr7:79986845-79986864	MsG0780040612.01.T01:CDS	45.0%
	GAACGTGACTCGAAACTTGT+GGG	-	Chr7:79986789-79986808	MsG0780040612.01.T01:CDS	45.0%
	GAGAAATGGCCATTGTTGGT+GGG	-	Chr7:79986955-79986974	MsG0780040612.01.T01:CDS	45.0%
	TACCAAAGGAGATGCCATTG+TGG	-	Chr7:79987037-79987056	MsG0780040612.01.T01:CDS	45.0%
	TGAACGTGACTCGAAACTTG+TGG	-	Chr7:79986788-79986807	MsG0780040612.01.T01:CDS	45.0%
	TGACTCGAAACTTGTGGGTA+AGG	-	Chr7:79986794-79986813	MsG0780040612.01.T01:CDS	45.0%
	TTCCACAATGGCATCTCCTT+TGG	+	Chr7:79987042-79987061	None:intergenic	45.0%
!	ACAAGTTTCGAGTCACGTTC+AGG	+	Chr7:79986789-79986808	None:intergenic	45.0%
	AAATGCGTTGCCATTCGGGA+CGG	-	Chr7:79986731-79986750	MsG0780040612.01.T01:CDS	50.0%
	ATCTCCATCACTGAACGCCA+TGG	+	Chr7:79986877-79986896	None:intergenic	50.0%
	ATGACCATGGCGTTCAGTGA+TGG	-	Chr7:79986870-79986889	MsG0780040612.01.T01:CDS	50.0%
	GTCAGGACCAAAACCTAGCA+TGG	-	Chr7:79986677-79986696	MsG0780040612.01.T01:CDS	50.0%
	TGTAGCTGAGCCTAATGGCA+AGG	-	Chr7:79986704-79986723	MsG0780040612.01.T01:CDS	50.0%
!	GGAGCTTTTCGTTTCGTACG+TGG	-	Chr7:79986981-79987000	MsG0780040612.01.T01:CDS	50.0%
	AGCTCCAGTCCCACCAACAA+TGG	+	Chr7:79986967-79986986	None:intergenic	55.0%
	ATGGATGACCCATTGACGGC+TGG	-	Chr7:79986762-79986781	MsG0780040612.01.T01:CDS	55.0%
	GGCGATGGATGACCCATTGA+CGG	-	Chr7:79986758-79986777	MsG0780040612.01.T01:CDS	55.0%
	GTTCAGGACCAGCCGTCAAT+GGG	+	Chr7:79986773-79986792	None:intergenic	55.0%
!	ATGGCCATTGTTGGTGGGAC+TGG	-	Chr7:79986960-79986979	MsG0780040612.01.T01:CDS	55.0%
	ATCGCCACAACCGTCCCGAA+TGG	+	Chr7:79986744-79986763	None:intergenic	60.0%
	ATTCGGGACGGTTGTGGCGA+TGG	-	Chr7:79986743-79986762	MsG0780040612.01.T01:CDS	60.0%
	CGTTCAGGACCAGCCGTCAA+TGG	+	Chr7:79986774-79986793	None:intergenic	60.0%
	GTTGCCATTCGGGACGGTTG+TGG	-	Chr7:79986737-79986756	MsG0780040612.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	79986618	79987088	79986618	ID=MsG0780040612.01;Name=MsG0780040612.01
Chr7	mRNA	79986618	79987088	79986618	ID=MsG0780040612.01.T01;Parent=MsG0780040612.01;Name=MsG0780040612.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|156
Chr7	exon	79986618	79987088	79986618	ID=MsG0780040612.01.T01:exon:16388;Parent=MsG0780040612.01.T01
Chr7	CDS	79986618	79987088	79986618	ID=MsG0780040612.01.T01:cds;Parent=MsG0780040612.01.T01

Gene Sequence

>MsG0780040612.01.T01

ATGCTGGGTTTTCAAGAAGAGAAATTCACTCACCTTCACTTTTACTTTCACGACATTGTGTCAGGACCAAAACCTAGCATGGTATTTGTAGCTGAGCCTAATGGCAAGGTAAAAAATGCGTTGCCATTCGGGACGGTTGTGGCGATGGATGACCCATTGACGGCTGGTCCTGAACGTGACTCGAAACTTGTGGGTAAGGCTCAAGGAATTTACACTTCTATATCACAAGAGGAGATGGGACTAATGATGGTGATGACCATGGCGTTCAGTGATGGAGATTTCAATGGAAGTACACTTAGCATATTGGGCAGAAACATGATCATGAGTGAACCTGTTAGAGAAATGGCCATTGTTGGTGGGACTGGAGCTTTTCGTTTCGTACGTGGTTATGCTCAAGCTAAATTTTATTCTGTTGATTATACCAAAGGAGATGCCATTGTGGAATATGATGTATTCGTATTCCATTATTAG

Protein sequence

>MsG0780040612.01.T01

MLGFQEEKFTHLHFYFHDIVSGPKPSMVFVAEPNGKVKNALPFGTVVAMDDPLTAGPERDSKLVGKAQGIYTSISQEEMGLMMVMTMAFSDGDFNGSTLSILGRNMIMSEPVREMAIVGGTGAFRFVRGYAQAKFYSVDYTKGDAIVEYDVFVFHY*