AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040614.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040614.01.T01 MTR_7g093820 99.359 156 1 0 1 156 39 194 4.38e-115 323
MsG0780040614.01.T01 MTR_7g093850 98.077 156 3 0 1 156 39 194 1.33e-113 320
MsG0780040614.01.T01 MTR_7g093830 97.436 156 4 0 1 156 38 193 1.85e-112 317
MsG0780040614.01.T01 MTR_7g093790 80.128 156 31 0 1 156 36 191 1.32e-94 272
MsG0780040614.01.T01 MTR_7g093870 60.000 160 58 3 2 156 35 193 2.46e-65 199
MsG0780040614.01.T01 MTR_4g013350 52.866 157 72 2 1 156 42 197 2.93e-57 177
MsG0780040614.01.T01 MTR_4g013345 52.866 157 72 2 1 156 41 196 5.35e-56 174
MsG0780040614.01.T01 MTR_1g054525 55.263 152 65 2 5 156 44 192 1.18e-54 171
MsG0780040614.01.T01 MTR_4g013335 50.955 157 73 3 1 156 43 196 2.90e-54 170
MsG0780040614.01.T01 MTR_4g013330 50.943 159 74 3 1 156 42 199 9.06e-54 169
MsG0780040614.01.T01 MTR_4g013320 51.316 152 73 1 5 156 49 199 1.26e-53 168
MsG0780040614.01.T01 MTR_4g013325 53.425 146 67 1 11 156 55 199 3.70e-53 167
MsG0780040614.01.T01 MTR_4g073950 52.941 153 70 2 5 156 41 192 2.34e-51 162
MsG0780040614.01.T01 MTR_4g013310 51.316 152 71 2 5 156 49 197 3.00e-50 160
MsG0780040614.01.T01 MTR_3g105640 52.288 153 70 3 6 156 37 188 8.10e-50 158
MsG0780040614.01.T01 MTR_3g105630 50.000 154 73 3 6 156 37 189 3.19e-49 157
MsG0780040614.01.T01 MTR_7g070390 51.974 152 71 2 5 156 43 192 2.44e-47 152
MsG0780040614.01.T01 MTR_4g013385 46.053 152 80 2 5 156 41 190 1.40e-44 145
MsG0780040614.01.T01 MTR_0433s0040 46.053 152 80 2 5 156 41 190 1.40e-44 145
MsG0780040614.01.T01 MTR_5g096120 49.677 155 71 4 7 156 37 189 1.81e-44 145
MsG0780040614.01.T01 MTR_1g056370 48.299 147 73 3 10 155 41 185 9.19e-43 140
MsG0780040614.01.T01 MTR_4g013770 42.568 148 83 2 10 156 45 191 2.67e-41 137
MsG0780040614.01.T01 MTR_3g034030 46.358 151 76 4 7 156 39 185 5.14e-40 133
MsG0780040614.01.T01 MTR_4g078885 45.395 152 78 4 6 156 38 185 1.96e-39 132
MsG0780040614.01.T01 MTR_4g013355 40.132 152 80 1 5 156 39 179 5.15e-36 123
MsG0780040614.01.T01 MTR_4g122110 40.397 151 89 1 6 156 31 180 4.48e-30 107
MsG0780040614.01.T01 MTR_4g122130 40.397 151 89 1 6 156 31 180 1.05e-29 107
MsG0780040614.01.T01 MTR_4g049570 32.215 149 100 1 3 151 26 173 2.95e-21 85.1
MsG0780040614.01.T01 MTR_4g049550 30.822 146 100 1 6 151 29 173 2.11e-20 83.2
MsG0780040614.01.T01 MTR_1g046800 41.053 95 54 2 40 134 133 225 1.17e-13 66.6
MsG0780040614.01.T01 MTR_1g046490 38.947 95 56 2 40 134 119 211 1.17e-13 66.2
MsG0780040614.01.T01 MTR_1g046500 40.000 95 55 2 40 134 133 225 1.66e-13 66.2
MsG0780040614.01.T01 MTR_1g115510 36.154 130 75 5 33 155 225 353 9.35e-13 64.7
MsG0780040614.01.T01 MTR_4g062520 37.383 107 66 1 33 139 177 282 1.56e-12 63.9
MsG0780040614.01.T01 MTR_4g062470 37.383 107 66 1 33 139 178 283 1.99e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040614.01.T01 AT5G42500 53.595 153 68 2 3 155 36 185 5.13e-54 169
MsG0780040614.01.T01 AT5G42510 51.634 153 71 2 3 155 33 182 2.02e-51 162
MsG0780040614.01.T01 AT1G65870 52.288 153 72 1 3 155 38 189 2.03e-51 162
MsG0780040614.01.T01 AT1G22900 51.634 153 71 2 3 155 44 193 1.11e-49 158
MsG0780040614.01.T01 AT1G58170 46.452 155 82 1 2 156 32 185 2.46e-48 154
MsG0780040614.01.T01 AT5G49040 46.053 152 81 1 5 156 41 191 2.46e-47 152
MsG0780040614.01.T01 AT1G55210 46.497 157 79 3 1 156 35 187 3.34e-47 152
MsG0780040614.01.T01 AT1G55210 46.497 157 79 3 1 156 35 187 3.34e-47 152
MsG0780040614.01.T01 AT3G13650 45.455 154 80 2 3 156 37 186 4.99e-46 149
MsG0780040614.01.T01 AT2G21100 48.000 150 77 1 6 155 39 187 3.27e-43 141
MsG0780040614.01.T01 AT2G21100 48.000 150 77 1 6 155 39 187 3.27e-43 141
MsG0780040614.01.T01 AT3G13662 48.571 140 68 2 4 143 37 172 4.25e-43 140
MsG0780040614.01.T01 AT2G21110 44.371 151 79 2 10 156 37 186 2.30e-42 139
MsG0780040614.01.T01 AT4G38700 44.304 158 83 3 4 156 33 190 4.55e-40 134
MsG0780040614.01.T01 AT5G49030 47.692 130 67 1 5 134 41 169 1.18e-37 136
MsG0780040614.01.T01 AT3G58090 46.269 134 71 1 22 155 139 271 1.15e-34 122
MsG0780040614.01.T01 AT3G13660 44.538 119 65 1 38 156 8 125 2.11e-33 114
MsG0780040614.01.T01 AT4G23690 34.586 133 75 5 5 131 36 162 1.73e-13 65.1
MsG0780040614.01.T01 AT3G24020 35.789 95 59 2 40 134 126 218 8.77e-11 58.5

Find 41 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATGGCCATTTCTCTAAC+AGG 0.363166 7:+80007793 None:intergenic
ACAAATACCATGCTAGGTTT+TGG 0.370876 7:+80008057 None:intergenic
GGAAGCACACTTAGCATATT+AGG 0.380466 7:-80007838 MsG0780040614.01.T01:CDS
ATGGCCATTGTTGGTGGGAC+TGG 0.413384 7:-80007781 MsG0780040614.01.T01:CDS
TATTCGGTTGATTATACAAA+AGG 0.426193 7:-80007718 MsG0780040614.01.T01:CDS
TACTTTCATGACATCGTGTC+GGG 0.426601 7:-80008081 MsG0780040614.01.T01:CDS
CGTTCTGGCCCAGCCGTCAA+TGG 0.431463 7:+80007970 None:intergenic
AGCCTATCTCCAACAATGTT+GGG 0.432623 7:-80008138 None:intergenic
ATGGACGATCCATTGACGGC+TGG 0.438919 7:-80007979 MsG0780040614.01.T01:CDS
GAGTGAACCTGTTAGAGAAA+TGG 0.463422 7:-80007800 MsG0780040614.01.T01:CDS
ACAAGTTTCGAGTCACGTTC+TGG 0.465414 7:+80007955 None:intergenic
TTCTATATCACAAGAGGAGA+TGG 0.477985 7:-80007908 MsG0780040614.01.T01:CDS
TGGACGATCCATTGACGGCT+GGG 0.512893 7:-80007978 MsG0780040614.01.T01:CDS
TTTCGTACGTGGTTATGCTC+AGG 0.517603 7:-80007749 MsG0780040614.01.T01:CDS
ATTCGGGACAGTTGTGGCGA+TGG 0.519713 7:-80007998 MsG0780040614.01.T01:CDS
AGAGGAGATGGGACTAATGA+TGG 0.528699 7:-80007896 MsG0780040614.01.T01:CDS
TCAGCTACAAATACCATGCT+AGG 0.528865 7:+80008051 None:intergenic
TGACTCGAAACTTGTGGGTA+AGG 0.534298 7:-80007947 MsG0780040614.01.T01:CDS
GAACGTGACTCGAAACTTGT+GGG 0.536601 7:-80007952 MsG0780040614.01.T01:CDS
CTTGCCATTCGGGACAGTTG+TGG 0.537319 7:-80008004 MsG0780040614.01.T01:CDS
GGCGATGGACGATCCATTGA+CGG 0.538554 7:-80007983 MsG0780040614.01.T01:CDS
GTTAGAGAAATGGCCATTGT+TGG 0.545345 7:-80007790 MsG0780040614.01.T01:CDS
AAACTTGTGGGTAAGGCTCA+AGG 0.545821 7:-80007940 MsG0780040614.01.T01:CDS
TTACACTTCTATATCACAAG+AGG 0.558364 7:-80007914 MsG0780040614.01.T01:CDS
ATGACCATGGCATTCACTGA+TGG 0.562304 7:-80007871 MsG0780040614.01.T01:CDS
TTACTTTCATGACATCGTGT+CGG 0.568079 7:-80008082 MsG0780040614.01.T01:CDS
AGAGAAATGGCCATTGTTGG+TGG 0.569066 7:-80007787 MsG0780040614.01.T01:CDS
AGCTCCAGTCCCACCAACAA+TGG 0.574074 7:+80007777 None:intergenic
TCTATATCACAAGAGGAGAT+GGG 0.592301 7:-80007907 MsG0780040614.01.T01:CDS
AGAACGTGACTCGAAACTTG+TGG 0.594960 7:-80007953 MsG0780040614.01.T01:CDS
GAGAAATGGCCATTGTTGGT+GGG 0.599344 7:-80007786 MsG0780040614.01.T01:CDS
CACAACTGTCCCGAATGGCA+AGG 0.608603 7:+80008005 None:intergenic
GTCGGGACCAAAACCTAGCA+TGG 0.611064 7:-80008064 MsG0780040614.01.T01:CDS
ATCGCCACAACTGTCCCGAA+TGG 0.611420 7:+80008000 None:intergenic
ATCTCCATCAGTGAATGCCA+TGG 0.621162 7:+80007867 None:intergenic
TGTCATGAAAGTAAAAGTGA+AGG 0.621515 7:+80008091 None:intergenic
TACAAAAGGAGATGCCATTG+TGG 0.627394 7:-80007704 MsG0780040614.01.T01:CDS
TGTAGCTGAACCCAATGGCA+AGG 0.648979 7:-80008037 MsG0780040614.01.T01:CDS
GTATTTGTAGCTGAACCCAA+TGG 0.653475 7:-80008042 MsG0780040614.01.T01:CDS
AAATATGTCATACTCCACAA+TGG 0.693639 7:+80007690 None:intergenic
ACTAATGATGGTGATGACCA+TGG 0.702330 7:-80007884 MsG0780040614.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATTCGGTTGATTATACAAA+AGG - Chr7:80008080-80008099 MsG0780040614.01.T01:CDS 25.0%
AAATATGTCATACTCCACAA+TGG + Chr7:80008111-80008130 None:intergenic 30.0%
TGTCATGAAAGTAAAAGTGA+AGG + Chr7:80007710-80007729 None:intergenic 30.0%
TTACACTTCTATATCACAAG+AGG - Chr7:80007884-80007903 MsG0780040614.01.T01:CDS 30.0%
! TTCACTGATGGAGATTTTAA+TGG - Chr7:80007939-80007958 MsG0780040614.01.T01:CDS 30.0%
GTAAAAAATGCCTTGCCATT+CGG - Chr7:80007783-80007802 MsG0780040614.01.T01:CDS 35.0%
TAAAAAATGCCTTGCCATTC+GGG - Chr7:80007784-80007803 MsG0780040614.01.T01:CDS 35.0%
TCTATATCACAAGAGGAGAT+GGG - Chr7:80007891-80007910 MsG0780040614.01.T01:CDS 35.0%
TTACTTTCATGACATCGTGT+CGG - Chr7:80007716-80007735 MsG0780040614.01.T01:CDS 35.0%
TTCTATATCACAAGAGGAGA+TGG - Chr7:80007890-80007909 MsG0780040614.01.T01:CDS 35.0%
! ACAAATACCATGCTAGGTTT+TGG + Chr7:80007744-80007763 None:intergenic 35.0%
!!! GCATTTTTTACCTTGCCATT+GGG + Chr7:80007774-80007793 None:intergenic 35.0%
!!! TGCTCAGGCTAAGTTTTATT+CGG - Chr7:80008064-80008083 MsG0780040614.01.T01:CDS 35.0%
ACAATGGCCATTTCTCTAAC+AGG + Chr7:80008008-80008027 None:intergenic 40.0%
GAGTGAACCTGTTAGAGAAA+TGG - Chr7:80007998-80008017 MsG0780040614.01.T01:CDS 40.0%
GTATTTGTAGCTGAACCCAA+TGG - Chr7:80007756-80007775 MsG0780040614.01.T01:CDS 40.0%
GTTAGAGAAATGGCCATTGT+TGG - Chr7:80008008-80008027 MsG0780040614.01.T01:CDS 40.0%
TACAAAAGGAGATGCCATTG+TGG - Chr7:80008094-80008113 MsG0780040614.01.T01:CDS 40.0%
TACTTTCATGACATCGTGTC+GGG - Chr7:80007717-80007736 MsG0780040614.01.T01:CDS 40.0%
TCAGCTACAAATACCATGCT+AGG + Chr7:80007750-80007769 None:intergenic 40.0%
! ACTAATGATGGTGATGACCA+TGG - Chr7:80007914-80007933 MsG0780040614.01.T01:CDS 40.0%
! GGAAGCACACTTAGCATATT+AGG - Chr7:80007960-80007979 MsG0780040614.01.T01:CDS 40.0%
!!! GGCATTTTTTACCTTGCCAT+TGG + Chr7:80007775-80007794 None:intergenic 40.0%
AAACTTGTGGGTAAGGCTCA+AGG - Chr7:80007858-80007877 MsG0780040614.01.T01:CDS 45.0%
AGAACGTGACTCGAAACTTG+TGG - Chr7:80007845-80007864 MsG0780040614.01.T01:CDS 45.0%
AGAGAAATGGCCATTGTTGG+TGG - Chr7:80008011-80008030 MsG0780040614.01.T01:CDS 45.0%
AGAGGAGATGGGACTAATGA+TGG - Chr7:80007902-80007921 MsG0780040614.01.T01:CDS 45.0%
ATCTCCATCAGTGAATGCCA+TGG + Chr7:80007934-80007953 None:intergenic 45.0%
ATGACCATGGCATTCACTGA+TGG - Chr7:80007927-80007946 MsG0780040614.01.T01:CDS 45.0%
GAACGTGACTCGAAACTTGT+GGG - Chr7:80007846-80007865 MsG0780040614.01.T01:CDS 45.0%
GAGAAATGGCCATTGTTGGT+GGG - Chr7:80008012-80008031 MsG0780040614.01.T01:CDS 45.0%
TGACTCGAAACTTGTGGGTA+AGG - Chr7:80007851-80007870 MsG0780040614.01.T01:CDS 45.0%
TTTCGTACGTGGTTATGCTC+AGG - Chr7:80008049-80008068 MsG0780040614.01.T01:CDS 45.0%
! ACAAGTTTCGAGTCACGTTC+TGG + Chr7:80007846-80007865 None:intergenic 45.0%
TGTAGCTGAACCCAATGGCA+AGG - Chr7:80007761-80007780 MsG0780040614.01.T01:CDS 50.0%
! GGAGCTTTTCGTTTCGTACG+TGG - Chr7:80008038-80008057 MsG0780040614.01.T01:CDS 50.0%
AGCTCCAGTCCCACCAACAA+TGG + Chr7:80008024-80008043 None:intergenic 55.0%
ATCGCCACAACTGTCCCGAA+TGG + Chr7:80007801-80007820 None:intergenic 55.0%
ATGGACGATCCATTGACGGC+TGG - Chr7:80007819-80007838 MsG0780040614.01.T01:CDS 55.0%
ATTCGGGACAGTTGTGGCGA+TGG - Chr7:80007800-80007819 MsG0780040614.01.T01:CDS 55.0%
CACAACTGTCCCGAATGGCA+AGG + Chr7:80007796-80007815 None:intergenic 55.0%
CTTGCCATTCGGGACAGTTG+TGG - Chr7:80007794-80007813 MsG0780040614.01.T01:CDS 55.0%
GGCGATGGACGATCCATTGA+CGG - Chr7:80007815-80007834 MsG0780040614.01.T01:CDS 55.0%
GTCGGGACCAAAACCTAGCA+TGG - Chr7:80007734-80007753 MsG0780040614.01.T01:CDS 55.0%
TGGACGATCCATTGACGGCT+GGG - Chr7:80007820-80007839 MsG0780040614.01.T01:CDS 55.0%
! ATGGCCATTGTTGGTGGGAC+TGG - Chr7:80008017-80008036 MsG0780040614.01.T01:CDS 55.0%
CGTTCTGGCCCAGCCGTCAA+TGG + Chr7:80007831-80007850 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 80007675 80008145 80007675 ID=MsG0780040614.01;Name=MsG0780040614.01
Chr7 mRNA 80007675 80008145 80007675 ID=MsG0780040614.01.T01;Parent=MsG0780040614.01;Name=MsG0780040614.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|156
Chr7 exon 80007675 80008145 80007675 ID=MsG0780040614.01.T01:exon:16390;Parent=MsG0780040614.01.T01
Chr7 CDS 80007675 80008145 80007675 ID=MsG0780040614.01.T01:cds;Parent=MsG0780040614.01.T01
Gene Sequence

>MsG0780040614.01.T01

ATGTTGGGTTTTCAAGAAGAGAAATTCACTCACCTTCACTTTTACTTTCATGACATCGTGTCGGGACCAAAACCTAGCATGGTATTTGTAGCTGAACCCAATGGCAAGGTAAAAAATGCCTTGCCATTCGGGACAGTTGTGGCGATGGACGATCCATTGACGGCTGGGCCAGAACGTGACTCGAAACTTGTGGGTAAGGCTCAAGGAATTTACACTTCTATATCACAAGAGGAGATGGGACTAATGATGGTGATGACCATGGCATTCACTGATGGAGATTTTAATGGAAGCACACTTAGCATATTAGGCAGAAACATGATCATGAGTGAACCTGTTAGAGAAATGGCCATTGTTGGTGGGACTGGAGCTTTTCGTTTCGTACGTGGTTATGCTCAGGCTAAGTTTTATTCGGTTGATTATACAAAAGGAGATGCCATTGTGGAGTATGACATATTTGTGTTCCATTATTAG

Protein sequence

>MsG0780040614.01.T01

MLGFQEEKFTHLHFYFHDIVSGPKPSMVFVAEPNGKVKNALPFGTVVAMDDPLTAGPERDSKLVGKAQGIYTSISQEEMGLMMVMTMAFTDGDFNGSTLSILGRNMIMSEPVREMAIVGGTGAFRFVRGYAQAKFYSVDYTKGDAIVEYDIFVFHY*