AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040613.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040613.01.T01 MTR_7g093820 98.077 156 3 0 1 156 39 194 5.29e-114 321
MsG0780040613.01.T01 MTR_7g093850 96.795 156 5 0 1 156 39 194 1.42e-112 317
MsG0780040613.01.T01 MTR_7g093830 97.436 156 4 0 1 156 38 193 1.71e-112 317
MsG0780040613.01.T01 MTR_7g093790 80.128 156 31 0 1 156 36 191 2.77e-94 271
MsG0780040613.01.T01 MTR_7g093870 59.375 160 59 3 2 156 35 193 5.29e-65 198
MsG0780040613.01.T01 MTR_4g013350 52.866 157 72 2 1 156 42 197 4.58e-57 177
MsG0780040613.01.T01 MTR_4g013345 52.866 157 72 2 1 156 41 196 9.75e-56 174
MsG0780040613.01.T01 MTR_1g054525 54.605 152 66 2 5 156 44 192 9.93e-54 168
MsG0780040613.01.T01 MTR_4g013320 51.316 152 73 1 5 156 49 199 1.62e-53 168
MsG0780040613.01.T01 MTR_4g013335 50.318 157 74 3 1 156 43 196 1.63e-53 168
MsG0780040613.01.T01 MTR_4g013330 50.943 159 74 3 1 156 42 199 1.76e-53 168
MsG0780040613.01.T01 MTR_4g013325 52.740 146 68 1 11 156 55 199 1.67e-52 166
MsG0780040613.01.T01 MTR_4g073950 52.941 153 70 2 5 156 41 192 1.55e-51 163
MsG0780040613.01.T01 MTR_4g013310 51.316 152 71 2 5 156 49 197 4.30e-50 159
MsG0780040613.01.T01 MTR_3g105640 51.634 153 71 3 6 156 37 188 3.13e-49 157
MsG0780040613.01.T01 MTR_3g105630 49.351 154 74 3 6 156 37 189 9.70e-49 155
MsG0780040613.01.T01 MTR_7g070390 51.974 152 71 2 5 156 43 192 4.91e-47 151
MsG0780040613.01.T01 MTR_4g013385 46.053 152 80 2 5 156 41 190 1.23e-44 145
MsG0780040613.01.T01 MTR_0433s0040 46.053 152 80 2 5 156 41 190 1.23e-44 145
MsG0780040613.01.T01 MTR_5g096120 49.032 155 72 4 7 156 37 189 5.74e-44 143
MsG0780040613.01.T01 MTR_1g056370 47.619 147 74 3 10 155 41 185 2.55e-42 139
MsG0780040613.01.T01 MTR_4g013770 42.568 148 83 2 10 156 45 191 1.91e-41 137
MsG0780040613.01.T01 MTR_3g034030 45.695 151 77 4 7 156 39 185 1.81e-39 132
MsG0780040613.01.T01 MTR_4g078885 44.737 152 79 4 6 156 38 185 6.53e-39 130
MsG0780040613.01.T01 MTR_4g013355 40.132 152 80 1 5 156 39 179 4.88e-36 123
MsG0780040613.01.T01 MTR_4g122110 39.073 151 91 1 6 156 31 180 4.92e-29 105
MsG0780040613.01.T01 MTR_4g122130 39.073 151 91 1 6 156 31 180 1.40e-28 103
MsG0780040613.01.T01 MTR_4g049570 32.215 149 100 1 3 151 26 173 2.33e-21 85.5
MsG0780040613.01.T01 MTR_4g049550 30.822 146 100 1 6 151 29 173 2.30e-20 82.8
MsG0780040613.01.T01 MTR_1g046490 38.947 95 56 2 40 134 119 211 1.24e-13 66.2
MsG0780040613.01.T01 MTR_1g046800 40.000 95 55 2 40 134 133 225 1.25e-13 66.6
MsG0780040613.01.T01 MTR_1g046500 40.000 95 55 2 40 134 133 225 1.73e-13 66.2
MsG0780040613.01.T01 MTR_4g062520 37.383 107 66 1 33 139 177 282 1.85e-12 63.9
MsG0780040613.01.T01 MTR_4g062470 37.383 107 66 1 33 139 178 283 2.24e-12 63.5
MsG0780040613.01.T01 MTR_1g115510 35.385 130 76 5 33 155 225 353 2.41e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040613.01.T01 AT5G42500 52.941 153 69 2 3 155 36 185 1.45e-53 167
MsG0780040613.01.T01 AT5G42510 50.980 153 72 2 3 155 33 182 4.10e-51 161
MsG0780040613.01.T01 AT1G65870 51.634 153 73 1 3 155 38 189 4.71e-51 161
MsG0780040613.01.T01 AT1G22900 50.980 153 72 2 3 155 44 193 2.97e-49 157
MsG0780040613.01.T01 AT1G58170 46.452 155 82 1 2 156 32 185 3.92e-48 154
MsG0780040613.01.T01 AT5G49040 46.053 152 81 1 5 156 41 191 3.52e-47 152
MsG0780040613.01.T01 AT1G55210 46.497 157 79 3 1 156 35 187 5.63e-47 151
MsG0780040613.01.T01 AT1G55210 46.497 157 79 3 1 156 35 187 5.63e-47 151
MsG0780040613.01.T01 AT3G13650 45.455 154 80 2 3 156 37 186 7.55e-46 148
MsG0780040613.01.T01 AT2G21100 48.000 150 77 1 6 155 39 187 9.66e-44 143
MsG0780040613.01.T01 AT2G21100 48.000 150 77 1 6 155 39 187 9.66e-44 143
MsG0780040613.01.T01 AT3G13662 47.857 140 69 2 4 143 37 172 2.56e-42 139
MsG0780040613.01.T01 AT2G21110 43.709 151 80 2 10 156 37 186 3.63e-42 139
MsG0780040613.01.T01 AT4G38700 43.671 158 84 3 4 156 33 190 1.01e-39 132
MsG0780040613.01.T01 AT5G49030 47.692 130 67 1 5 134 41 169 2.01e-37 135
MsG0780040613.01.T01 AT3G58090 45.522 134 72 1 22 155 139 271 3.53e-34 120
MsG0780040613.01.T01 AT3G13660 44.538 119 65 1 38 156 8 125 2.96e-33 114
MsG0780040613.01.T01 AT4G23690 34.586 133 75 5 5 131 36 162 8.79e-14 65.9
MsG0780040613.01.T01 AT4G11210 31.061 132 87 3 11 142 42 169 7.98e-11 57.8
MsG0780040613.01.T01 AT3G24020 35.789 95 59 2 40 134 126 218 8.34e-11 58.5

Find 44 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAAAACACCTAACTAATAA+TGG 0.327962 7:+79999185 None:intergenic
TGGAAGTACACTTAGCATAT+TGG 0.334562 7:-79999362 MsG0780040613.01.T01:CDS
ATGGATGACCCCTTGACAGC+TGG 0.343154 7:-79999502 MsG0780040613.01.T01:CDS
ACAATGGCCATTTCTCTAAC+AGG 0.363166 7:+79999316 None:intergenic
ACAAATACCATGCTAGGTTT+TGG 0.370876 7:+79999580 None:intergenic
GGAAGTACACTTAGCATATT+GGG 0.380807 7:-79999361 MsG0780040613.01.T01:CDS
AGCCTATCCCCAACAATGTT+GGG 0.408783 7:-79999661 None:intergenic
ATGGCCATTGTTGGTGGGAC+TGG 0.413384 7:-79999304 MsG0780040613.01.T01:CDS
ACGTTCGGGCCCAGCTGTCA+AGG 0.414456 7:+79999492 None:intergenic
TACTTTCATGACATCGTGTC+GGG 0.429084 7:-79999604 MsG0780040613.01.T01:CDS
TATTCCGTTGATTACACTAA+AGG 0.444889 7:-79999241 MsG0780040613.01.T01:CDS
GAGTGAACCTGTTAGAGAAA+TGG 0.463422 7:-79999323 MsG0780040613.01.T01:CDS
CGTTCGGGCCCAGCTGTCAA+GGG 0.473432 7:+79999493 None:intergenic
TTCTATATCACAAGAGGAGA+TGG 0.477985 7:-79999431 MsG0780040613.01.T01:CDS
TTCAGTGATGGAGATTTCAA+TGG 0.482811 7:-79999382 MsG0780040613.01.T01:CDS
TGGATGACCCCTTGACAGCT+GGG 0.499162 7:-79999501 MsG0780040613.01.T01:CDS
TGAATCAAAACTTGTGGGTA+AGG 0.505874 7:-79999470 MsG0780040613.01.T01:CDS
ATCGCCACAACTGTCCCAAA+TGG 0.528212 7:+79999523 None:intergenic
AGAGGAGATGGGACTAATGA+TGG 0.528699 7:-79999419 MsG0780040613.01.T01:CDS
TCAGCTACAAATACCATGCT+AGG 0.528865 7:+79999574 None:intergenic
ATTTGGGACAGTTGTGGCGA+TGG 0.529221 7:-79999521 MsG0780040613.01.T01:CDS
GTTAGAGAAATGGCCATTGT+TGG 0.545345 7:-79999313 MsG0780040613.01.T01:CDS
ATGACCATGGCGTTCAGTGA+TGG 0.546366 7:-79999394 MsG0780040613.01.T01:CDS
TTTCGTACGTGGGTATGCTC+AGG 0.546620 7:-79999272 MsG0780040613.01.T01:CDS
AAACTTGTGGGTAAGGCTCA+AGG 0.547993 7:-79999463 MsG0780040613.01.T01:CDS
TTACACTTCTATATCACAAG+AGG 0.558364 7:-79999437 MsG0780040613.01.T01:CDS
CTTGCCATTTGGGACAGTTG+TGG 0.563355 7:-79999527 MsG0780040613.01.T01:CDS
GAACGTGAATCAAAACTTGT+GGG 0.564741 7:-79999475 MsG0780040613.01.T01:CDS
AGAGAAATGGCCATTGTTGG+TGG 0.569066 7:-79999310 MsG0780040613.01.T01:CDS
AGCTCCAGTCCCACCAACAA+TGG 0.574074 7:+79999300 None:intergenic
TTACTTTCATGACATCGTGT+CGG 0.584715 7:-79999605 MsG0780040613.01.T01:CDS
GTTCGGGCCCAGCTGTCAAG+GGG 0.587949 7:+79999494 None:intergenic
CACTAAAGGAGATGCCATTG+TGG 0.590470 7:-79999227 MsG0780040613.01.T01:CDS
TCTATATCACAAGAGGAGAT+GGG 0.592301 7:-79999430 MsG0780040613.01.T01:CDS
ATCTCCTTTAGTGTAATCAA+CGG 0.597699 7:+79999237 None:intergenic
CACAACTGTCCCAAATGGCA+AGG 0.598704 7:+79999528 None:intergenic
GAGAAATGGCCATTGTTGGT+GGG 0.599344 7:-79999309 MsG0780040613.01.T01:CDS
ATCTCCATCACTGAACGCCA+TGG 0.609829 7:+79999390 None:intergenic
GTCGGGACCAAAACCTAGCA+TGG 0.611064 7:-79999587 MsG0780040613.01.T01:CDS
CGAACGTGAATCAAAACTTG+TGG 0.618950 7:-79999476 MsG0780040613.01.T01:CDS
TGTAGCTGAACCCAATGGCA+AGG 0.648979 7:-79999560 MsG0780040613.01.T01:CDS
GTATTTGTAGCTGAACCCAA+TGG 0.653475 7:-79999565 MsG0780040613.01.T01:CDS
AAATATGTCATATTCCACAA+TGG 0.686079 7:+79999213 None:intergenic
ACTAATGATGGTGATGACCA+TGG 0.699161 7:-79999407 MsG0780040613.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATATGTCATATTCCACAA+TGG + Chr7:79999634-79999653 None:intergenic 25.0%
! TGTCATGAAAGTAAAAATGA+AGG + Chr7:79999233-79999252 None:intergenic 25.0%
ATCTCCTTTAGTGTAATCAA+CGG + Chr7:79999610-79999629 None:intergenic 30.0%
TTACACTTCTATATCACAAG+AGG - Chr7:79999407-79999426 MsG0780040613.01.T01:CDS 30.0%
! TAAAAAATGCCTTGCCATTT+GGG - Chr7:79999307-79999326 MsG0780040613.01.T01:CDS 30.0%
! TATTCCGTTGATTACACTAA+AGG - Chr7:79999603-79999622 MsG0780040613.01.T01:CDS 30.0%
GAACGTGAATCAAAACTTGT+GGG - Chr7:79999369-79999388 MsG0780040613.01.T01:CDS 35.0%
GTAAAAAATGCCTTGCCATT+TGG - Chr7:79999306-79999325 MsG0780040613.01.T01:CDS 35.0%
TCTATATCACAAGAGGAGAT+GGG - Chr7:79999414-79999433 MsG0780040613.01.T01:CDS 35.0%
TGAATCAAAACTTGTGGGTA+AGG - Chr7:79999374-79999393 MsG0780040613.01.T01:CDS 35.0%
TTACTTTCATGACATCGTGT+CGG - Chr7:79999239-79999258 MsG0780040613.01.T01:CDS 35.0%
TTCTATATCACAAGAGGAGA+TGG - Chr7:79999413-79999432 MsG0780040613.01.T01:CDS 35.0%
! ACAAATACCATGCTAGGTTT+TGG + Chr7:79999267-79999286 None:intergenic 35.0%
! GGAAGTACACTTAGCATATT+GGG - Chr7:79999483-79999502 MsG0780040613.01.T01:CDS 35.0%
! TGGAAGTACACTTAGCATAT+TGG - Chr7:79999482-79999501 MsG0780040613.01.T01:CDS 35.0%
! TTCAGTGATGGAGATTTCAA+TGG - Chr7:79999462-79999481 MsG0780040613.01.T01:CDS 35.0%
!!! ACAAGTTTTGATTCACGTTC+GGG + Chr7:79999369-79999388 None:intergenic 35.0%
!!! CACAAGTTTTGATTCACGTT+CGG + Chr7:79999370-79999389 None:intergenic 35.0%
!!! GCATTTTTTACCTTGCCATT+GGG + Chr7:79999297-79999316 None:intergenic 35.0%
ACAATGGCCATTTCTCTAAC+AGG + Chr7:79999531-79999550 None:intergenic 40.0%
CGAACGTGAATCAAAACTTG+TGG - Chr7:79999368-79999387 MsG0780040613.01.T01:CDS 40.0%
GAGTGAACCTGTTAGAGAAA+TGG - Chr7:79999521-79999540 MsG0780040613.01.T01:CDS 40.0%
GTATTTGTAGCTGAACCCAA+TGG - Chr7:79999279-79999298 MsG0780040613.01.T01:CDS 40.0%
GTTAGAGAAATGGCCATTGT+TGG - Chr7:79999531-79999550 MsG0780040613.01.T01:CDS 40.0%
TACTTTCATGACATCGTGTC+GGG - Chr7:79999240-79999259 MsG0780040613.01.T01:CDS 40.0%
TCAGCTACAAATACCATGCT+AGG + Chr7:79999273-79999292 None:intergenic 40.0%
! ACTAATGATGGTGATGACCA+TGG - Chr7:79999437-79999456 MsG0780040613.01.T01:CDS 40.0%
!!! GGCATTTTTTACCTTGCCAT+TGG + Chr7:79999298-79999317 None:intergenic 40.0%
AAACTTGTGGGTAAGGCTCA+AGG - Chr7:79999381-79999400 MsG0780040613.01.T01:CDS 45.0%
AGAGAAATGGCCATTGTTGG+TGG - Chr7:79999534-79999553 MsG0780040613.01.T01:CDS 45.0%
AGAGGAGATGGGACTAATGA+TGG - Chr7:79999425-79999444 MsG0780040613.01.T01:CDS 45.0%
CACTAAAGGAGATGCCATTG+TGG - Chr7:79999617-79999636 MsG0780040613.01.T01:CDS 45.0%
GAGAAATGGCCATTGTTGGT+GGG - Chr7:79999535-79999554 MsG0780040613.01.T01:CDS 45.0%
! GAGCTTTTCGTTTCGTACGT+GGG - Chr7:79999562-79999581 MsG0780040613.01.T01:CDS 45.0%
ATCGCCACAACTGTCCCAAA+TGG + Chr7:79999324-79999343 None:intergenic 50.0%
ATCTCCATCACTGAACGCCA+TGG + Chr7:79999457-79999476 None:intergenic 50.0%
ATGACCATGGCGTTCAGTGA+TGG - Chr7:79999450-79999469 MsG0780040613.01.T01:CDS 50.0%
CACAACTGTCCCAAATGGCA+AGG + Chr7:79999319-79999338 None:intergenic 50.0%
TGTAGCTGAACCCAATGGCA+AGG - Chr7:79999284-79999303 MsG0780040613.01.T01:CDS 50.0%
TTTCGTACGTGGGTATGCTC+AGG - Chr7:79999572-79999591 MsG0780040613.01.T01:CDS 50.0%
! ATTTGGGACAGTTGTGGCGA+TGG - Chr7:79999323-79999342 MsG0780040613.01.T01:CDS 50.0%
! CTTGCCATTTGGGACAGTTG+TGG - Chr7:79999317-79999336 MsG0780040613.01.T01:CDS 50.0%
! GGAGCTTTTCGTTTCGTACG+TGG - Chr7:79999561-79999580 MsG0780040613.01.T01:CDS 50.0%
AGCTCCAGTCCCACCAACAA+TGG + Chr7:79999547-79999566 None:intergenic 55.0%
ATGGATGACCCCTTGACAGC+TGG - Chr7:79999342-79999361 MsG0780040613.01.T01:CDS 55.0%
GTCGGGACCAAAACCTAGCA+TGG - Chr7:79999257-79999276 MsG0780040613.01.T01:CDS 55.0%
TGGATGACCCCTTGACAGCT+GGG - Chr7:79999343-79999362 MsG0780040613.01.T01:CDS 55.0%
! ATGGCCATTGTTGGTGGGAC+TGG - Chr7:79999540-79999559 MsG0780040613.01.T01:CDS 55.0%
ACGTTCGGGCCCAGCTGTCA+AGG + Chr7:79999355-79999374 None:intergenic 65.0%
CGTTCGGGCCCAGCTGTCAA+GGG + Chr7:79999354-79999373 None:intergenic 65.0%
GTTCGGGCCCAGCTGTCAAG+GGG + Chr7:79999353-79999372 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 79999198 79999668 79999198 ID=MsG0780040613.01;Name=MsG0780040613.01
Chr7 mRNA 79999198 79999668 79999198 ID=MsG0780040613.01.T01;Parent=MsG0780040613.01;Name=MsG0780040613.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|156
Chr7 exon 79999198 79999668 79999198 ID=MsG0780040613.01.T01:exon:16389;Parent=MsG0780040613.01.T01
Chr7 CDS 79999198 79999668 79999198 ID=MsG0780040613.01.T01:cds;Parent=MsG0780040613.01.T01
Gene Sequence

>MsG0780040613.01.T01

ATGTTGGGTTTTCAAGAAGAGAAATTCACTCACCTTCATTTTTACTTTCATGACATCGTGTCGGGACCAAAACCTAGCATGGTATTTGTAGCTGAACCCAATGGCAAGGTAAAAAATGCCTTGCCATTTGGGACAGTTGTGGCGATGGATGACCCCTTGACAGCTGGGCCCGAACGTGAATCAAAACTTGTGGGTAAGGCTCAAGGAATTTACACTTCTATATCACAAGAGGAGATGGGACTAATGATGGTGATGACCATGGCGTTCAGTGATGGAGATTTCAATGGAAGTACACTTAGCATATTGGGCAGAAACATGATCATGAGTGAACCTGTTAGAGAAATGGCCATTGTTGGTGGGACTGGAGCTTTTCGTTTCGTACGTGGGTATGCTCAGGCTAAGTTTTATTCCGTTGATTACACTAAAGGAGATGCCATTGTGGAATATGACATATTTGTGTTCCATTATTAG

Protein sequence

>MsG0780040613.01.T01

MLGFQEEKFTHLHFYFHDIVSGPKPSMVFVAEPNGKVKNALPFGTVVAMDDPLTAGPERESKLVGKAQGIYTSISQEEMGLMMVMTMAFSDGDFNGSTLSILGRNMIMSEPVREMAIVGGTGAFRFVRGYAQAKFYSVDYTKGDAIVEYDIFVFHY*