AlfalfaGEDB | MsG0080048838.01

M. sativa cultivar ZhongmuNo.1 / MsG0080048838.01

KEGG: -
GO: -
PFAM: PF03018

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048838.01.T01	XP_003623402.1	96.354	192	7	0	1	192	1	192	2.85E-133	381

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048838.01.T01	Q9C523	68.182	154	48	1	40	192	32	185	2.04E-75	227

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048838.01.T01	G7KU38	96.354	192	7	0	1	192	1	192	1.36e-133	381

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048838.01	MsG0180000241.01	0.810281	1.246440e-50	7.481987e-48
MsG0080048838.01	MsG0280008165.01	0.810873	9.284026e-51	5.661840e-48
MsG0080048838.01	MsG0280008171.01	0.822214	2.670783e-53	2.221103e-50
MsG0080048838.01	MsG0380015486.01	0.814028	1.897960e-51	1.259442e-48
MsG0080048838.01	MsG0880041864.01	0.816639	4.984718e-52	3.551500e-49
MsG0080048838.01	MsG0880043298.01	0.809016	2.330577e-50	1.352957e-47

PPI

Gene1	Gene2	Type
MsG0780040614.01	MsG0080048838.01	PPI
MsG0780040609.01	MsG0080048838.01	PPI
MsG0080048537.01	MsG0080048838.01	PPI
MsG0080048537.01	MsG0080048838.01	PPI
MsG0780040615.01	MsG0080048838.01	PPI
MsG0080048838.01	MsG0480018519.01	PPI
MsG0480018518.01	MsG0080048838.01	PPI
MsG0080048537.01	MsG0080048838.01	PPI
MsG0780040612.01	MsG0080048838.01	PPI
MsG0780040611.01	MsG0080048838.01	PPI
MsG0780040613.01	MsG0080048838.01	PPI
MsG0080048838.01	MsG0780040610.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048838.01.T01	MTR_7g070390	96.354	192	7	0	1	192	1	192	3.45e-137	381
MsG0080048838.01.T01	MTR_4g013350	68.927	177	53	2	17	192	22	197	5.11e-88	257
MsG0080048838.01.T01	MTR_4g013345	69.101	178	53	2	16	192	20	196	1.73e-86	253
MsG0080048838.01.T01	MTR_4g013320	71.345	171	47	2	23	192	30	199	3.54e-86	252
MsG0080048838.01.T01	MTR_4g013335	68.824	170	51	2	23	192	29	196	2.36e-84	248
MsG0080048838.01.T01	MTR_4g013330	68.605	172	50	2	24	192	29	199	5.04e-84	247
MsG0080048838.01.T01	MTR_4g013325	69.231	169	49	2	25	192	33	199	3.66e-81	239
MsG0080048838.01.T01	MTR_4g013310	66.082	171	54	3	23	192	30	197	1.26e-78	233
MsG0080048838.01.T01	MTR_4g013385	57.447	188	77	1	5	192	6	190	5.56e-76	226
MsG0080048838.01.T01	MTR_0433s0040	57.447	188	77	1	5	192	6	190	5.56e-76	226
MsG0080048838.01.T01	MTR_4g013770	56.983	179	72	2	16	192	16	191	4.55e-68	206
MsG0080048838.01.T01	MTR_1g054525	49.479	192	88	5	5	192	6	192	5.70e-59	183
MsG0080048838.01.T01	MTR_4g013355	47.619	189	85	3	5	192	4	179	2.78e-57	178
MsG0080048838.01.T01	MTR_4g073950	48.235	170	81	2	30	192	23	192	2.47e-53	169
MsG0080048838.01.T01	MTR_7g093870	51.282	156	70	2	43	192	38	193	2.11e-52	167
MsG0080048838.01.T01	MTR_7g093850	50.000	168	78	4	28	192	30	194	1.35e-49	159
MsG0080048838.01.T01	MTR_7g093790	44.385	187	97	4	11	192	7	191	1.62e-49	159
MsG0080048838.01.T01	MTR_1g056370	43.915	189	93	3	7	191	6	185	1.62e-49	159
MsG0080048838.01.T01	MTR_7g093820	51.634	153	70	3	43	192	43	194	3.70e-49	158
MsG0080048838.01.T01	MTR_7g093830	44.845	194	100	5	2	192	4	193	9.36e-49	157
MsG0080048838.01.T01	MTR_3g105640	42.162	185	96	3	11	192	12	188	1.83e-46	151
MsG0080048838.01.T01	MTR_3g105630	40.000	190	102	3	7	192	8	189	1.80e-44	146
MsG0080048838.01.T01	MTR_4g078885	45.223	157	85	1	36	192	30	185	3.94e-44	145
MsG0080048838.01.T01	MTR_5g096120	41.026	195	98	5	5	192	5	189	6.57e-44	145
MsG0080048838.01.T01	MTR_3g034030	45.223	157	85	1	36	192	30	185	1.10e-43	144
MsG0080048838.01.T01	MTR_4g122110	33.117	154	99	2	40	192	30	180	2.54e-23	91.7
MsG0080048838.01.T01	MTR_4g122130	33.117	154	99	2	40	192	30	180	1.16e-21	87.4
MsG0080048838.01.T01	MTR_4g049550	26.667	150	109	1	39	187	24	173	1.32e-16	74.3
MsG0080048838.01.T01	MTR_8g099115	30.968	155	96	4	44	188	33	186	5.17e-16	72.8
MsG0080048838.01.T01	MTR_4g049570	29.577	142	94	2	50	190	35	171	2.76e-15	70.9
MsG0080048838.01.T01	MTR_8g099135	30.714	140	87	3	49	179	34	172	3.00e-15	70.9
MsG0080048838.01.T01	MTR_1g115510	34.454	119	71	3	80	191	235	353	6.23e-13	66.6
MsG0080048838.01.T01	MTR_1g046800	36.957	92	57	1	79	170	135	225	9.20e-12	62.4
MsG0080048838.01.T01	MTR_1g046500	36.957	92	57	1	79	170	135	225	1.23e-11	62.0
MsG0080048838.01.T01	MTR_1g046490	33.696	92	60	1	79	170	121	211	2.22e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048838.01.T01	AT1G58170	68.182	154	48	1	40	192	32	185	2.08e-76	227
MsG0080048838.01.T01	AT1G55210	54.301	186	82	3	7	192	5	187	4.33e-68	206
MsG0080048838.01.T01	AT1G55210	54.301	186	82	3	7	192	5	187	4.33e-68	206
MsG0080048838.01.T01	AT5G49040	53.646	192	84	3	5	192	1	191	7.82e-67	203
MsG0080048838.01.T01	AT3G13650	51.613	186	86	3	7	192	5	186	5.37e-65	198
MsG0080048838.01.T01	AT1G65870	52.318	151	71	1	42	191	39	189	7.07e-55	172
MsG0080048838.01.T01	AT5G49030	52.809	178	79	3	5	178	1	177	5.86e-52	179
MsG0080048838.01.T01	AT3G13660	57.600	125	53	0	68	192	1	125	5.87e-51	160
MsG0080048838.01.T01	AT1G22900	50.993	151	71	2	42	191	45	193	3.57e-49	158
MsG0080048838.01.T01	AT2G21100	46.711	152	80	1	41	191	36	187	7.84e-49	157
MsG0080048838.01.T01	AT2G21100	46.711	152	80	1	41	191	36	187	7.84e-49	157
MsG0080048838.01.T01	AT3G13662	52.027	148	68	2	31	178	27	171	8.27e-49	157
MsG0080048838.01.T01	AT5G42500	47.333	150	76	2	43	191	38	185	2.49e-48	156
MsG0080048838.01.T01	AT5G42510	46.667	150	77	2	43	191	35	182	8.07e-47	152
MsG0080048838.01.T01	AT4G38700	36.082	194	111	5	6	192	3	190	3.32e-38	130
MsG0080048838.01.T01	AT3G58090	50.000	110	55	0	82	191	162	271	6.90e-38	132
MsG0080048838.01.T01	AT2G21110	39.355	155	89	1	43	192	32	186	1.51e-35	123
MsG0080048838.01.T01	AT4G11210	28.743	167	112	4	27	192	24	184	1.05e-14	69.3
MsG0080048838.01.T01	AT1G64160	28.030	132	87	3	53	179	41	169	7.75e-11	58.9
MsG0080048838.01.T01	AT4G11180	28.814	177	111	6	16	187	13	179	9.74e-11	58.5

Find 41 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGTAGCACCATCACTATTT+TGG	0.149780	contig477end:+8144	MsG0080048838.01.T01:CDS
AATTCAGGTCCTAAGGTTAA+AGG	0.192169	contig477end:-8013	None:intergenic
AGACCTCAAAGAATTAAATT+TGG	0.222841	contig477end:+7847	MsG0080048838.01.T01:CDS
GAGTGGTGAAGAGGAGAAAA+AGG	0.264990	contig477end:-7769	None:intergenic
GTCTAATGAACGTACGAAAT+TGG	0.292324	contig477end:-7828	None:intergenic
ACCAATTTGGAACTCAATTC+AGG	0.296828	contig477end:-8028	None:intergenic
GTTAGGGAAATGCCTATAAT+TGG	0.317348	contig477end:+8190	MsG0080048838.01.T01:CDS
TAATCCATTTAAAATTCTAT+TGG	0.320311	contig477end:+7888	MsG0080048838.01.T01:CDS
ACCTGAATTGAGTTCCAAAT+TGG	0.324732	contig477end:+8027	MsG0080048838.01.T01:CDS
AGACCACTACCACCAATTAT+AGG	0.344419	contig477end:-8202	None:intergenic
TCTCCAAATTTAATTCTTTG+AGG	0.362138	contig477end:-7850	None:intergenic
TCCCTAACCTTATCATAAAC+AGG	0.368028	contig477end:-8175	None:intergenic
TTCAAAGGTGGTGGAACAAT+TGG	0.377643	contig477end:-7947	None:intergenic
ATTCAGGTCCTAAGGTTAAA+GGG	0.391274	contig477end:-8012	None:intergenic
ATCCTGTTTATGATAAGGTT+AGG	0.398598	contig477end:+8173	MsG0080048838.01.T01:CDS
ATGCTCAACTTAGAACTCAT+TGG	0.409836	contig477end:+8245	MsG0080048838.01.T01:CDS
GAGGAATCCTGTTTATGATA+AGG	0.426161	contig477end:+8168	MsG0080048838.01.T01:CDS
TATTGGCATGACATAGTTAG+TGG	0.428044	contig477end:+7905	MsG0080048838.01.T01:CDS
ATTGAGAACCCTTTAACCTT+AGG	0.428940	contig477end:+8004	MsG0080048838.01.T01:CDS
GGAACAATTGGTATTGAAGA+AGG	0.439349	contig477end:-7935	None:intergenic
TGCTTTGATTAAAGATGTAA+AGG	0.447724	contig477end:-7792	None:intergenic
AACTATAGCATCATATGTCT+TGG	0.451960	contig477end:-8275	None:intergenic
TCCTGTTTATGATAAGGTTA+GGG	0.471393	contig477end:+8174	MsG0080048838.01.T01:CDS
GAATTGAGTTCCAAATTGGT+TGG	0.472993	contig477end:+8031	MsG0080048838.01.T01:CDS
CAAAATTCATAGCCATGATA+AGG	0.483642	contig477end:-8099	None:intergenic
GGAACTCAATTCAGGTCCTA+AGG	0.484438	contig477end:-8020	None:intergenic
GCTTTGATTAAAGATGTAAA+GGG	0.491098	contig477end:-7791	None:intergenic
GAGCATATCCTCTAGCAAAT+CGG	0.492927	contig477end:-8228	None:intergenic
ATGCCTATAATTGGTGGTAG+TGG	0.495021	contig477end:+8199	MsG0080048838.01.T01:CDS
ATGTAAAGGGAGTGGTGAAG+AGG	0.506300	contig477end:-7778	None:intergenic
GAACAATTGGTATTGAAGAA+GGG	0.509427	contig477end:-7934	None:intergenic
TATCTCACTTTGTGATGTTG+AGG	0.532804	contig477end:-8074	None:intergenic
GATTAAAGATGTAAAGGGAG+TGG	0.544683	contig477end:-7786	None:intergenic
AGGGAAATGCCTATAATTGG+TGG	0.578046	contig477end:+8193	MsG0080048838.01.T01:CDS
AAATTGGTTGGAAAAGCACA+AGG	0.580992	contig477end:+8043	MsG0080048838.01.T01:CDS
GTTAACTAAACCAAAGGCAG+TGG	0.584234	contig477end:-7978	None:intergenic
AAGTGAGATAGACCTTATCA+TGG	0.591488	contig477end:+8087	MsG0080048838.01.T01:CDS
AATCATGTTAACTAAACCAA+AGG	0.600759	contig477end:-7984	None:intergenic
ATCATTGAAGGAAAGTACAA+TGG	0.628749	contig477end:+8124	MsG0080048838.01.T01:CDS
ATTCCTCCCCAAAATAGTGA+TGG	0.635430	contig477end:-8152	None:intergenic
AATTGGTTGGAAAAGCACAA+GGG	0.705686	contig477end:+8044	MsG0080048838.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAATCCATTTAAAATTCTAT+TGG	+	contig477end:7888-7907	MsG0080048838.01.T01:CDS	15.0%
!	AATCATGTTAACTAAACCAA+AGG	-	contig477end:7987-8006	None:intergenic	25.0%
!	AGACCTCAAAGAATTAAATT+TGG	+	contig477end:7847-7866	MsG0080048838.01.T01:CDS	25.0%
!	GCTTTGATTAAAGATGTAAA+GGG	-	contig477end:7794-7813	None:intergenic	25.0%
!	TCTCCAAATTTAATTCTTTG+AGG	-	contig477end:7853-7872	None:intergenic	25.0%
!	TGCTTTGATTAAAGATGTAA+AGG	-	contig477end:7795-7814	None:intergenic	25.0%
!!	CATGCCAATAGAATTTTAAA+TGG	-	contig477end:7895-7914	None:intergenic	25.0%
!!!	ATGAATTTTGCTATCATTGA+AGG	+	contig477end:8112-8131	MsG0080048838.01.T01:CDS	25.0%
	AACTATAGCATCATATGTCT+TGG	-	contig477end:8278-8297	None:intergenic	30.0%
	ATCCTGTTTATGATAAGGTT+AGG	+	contig477end:8173-8192	MsG0080048838.01.T01:CDS	30.0%
	CAAAATTCATAGCCATGATA+AGG	-	contig477end:8102-8121	None:intergenic	30.0%
	TCCTGTTTATGATAAGGTTA+GGG	+	contig477end:8174-8193	MsG0080048838.01.T01:CDS	30.0%
!	ATCATTGAAGGAAAGTACAA+TGG	+	contig477end:8124-8143	MsG0080048838.01.T01:CDS	30.0%
!	GAACAATTGGTATTGAAGAA+GGG	-	contig477end:7937-7956	None:intergenic	30.0%
	AAGTGAGATAGACCTTATCA+TGG	+	contig477end:8087-8106	MsG0080048838.01.T01:CDS	35.0%
	AATTCAGGTCCTAAGGTTAA+AGG	-	contig477end:8016-8035	None:intergenic	35.0%
	ACCTGAATTGAGTTCCAAAT+TGG	+	contig477end:8027-8046	MsG0080048838.01.T01:CDS	35.0%
	ATGCTCAACTTAGAACTCAT+TGG	+	contig477end:8245-8264	MsG0080048838.01.T01:CDS	35.0%
	ATTCAGGTCCTAAGGTTAAA+GGG	-	contig477end:8015-8034	None:intergenic	35.0%
	ATTGAGAACCCTTTAACCTT+AGG	+	contig477end:8004-8023	MsG0080048838.01.T01:CDS	35.0%
	GAATTGAGTTCCAAATTGGT+TGG	+	contig477end:8031-8050	MsG0080048838.01.T01:CDS	35.0%
	GAGGAATCCTGTTTATGATA+AGG	+	contig477end:8168-8187	MsG0080048838.01.T01:CDS	35.0%
	GATTAAAGATGTAAAGGGAG+TGG	-	contig477end:7789-7808	None:intergenic	35.0%
	GTCTAATGAACGTACGAAAT+TGG	-	contig477end:7831-7850	None:intergenic	35.0%
	GTTAGGGAAATGCCTATAAT+TGG	+	contig477end:8190-8209	MsG0080048838.01.T01:CDS	35.0%
	TATCTCACTTTGTGATGTTG+AGG	-	contig477end:8077-8096	None:intergenic	35.0%
	TATTGGCATGACATAGTTAG+TGG	+	contig477end:7905-7924	MsG0080048838.01.T01:CDS	35.0%
	TCCCTAACCTTATCATAAAC+AGG	-	contig477end:8178-8197	None:intergenic	35.0%
!	AAATTGGTTGGAAAAGCACA+AGG	+	contig477end:8043-8062	MsG0080048838.01.T01:CDS	35.0%
!	AATTGGTTGGAAAAGCACAA+GGG	+	contig477end:8044-8063	MsG0080048838.01.T01:CDS	35.0%
!	ACCAATTTGGAACTCAATTC+AGG	-	contig477end:8031-8050	None:intergenic	35.0%
!	GGAACAATTGGTATTGAAGA+AGG	-	contig477end:7938-7957	None:intergenic	35.0%
!	TTGTGCTTTTCCAACCAATT+TGG	-	contig477end:8044-8063	None:intergenic	35.0%
	AAAAACTCAACCACTGCCTT+TGG	+	contig477end:7968-7987	MsG0080048838.01.T01:CDS	40.0%
	AGACCACTACCACCAATTAT+AGG	-	contig477end:8205-8224	None:intergenic	40.0%
	AGGGAAATGCCTATAATTGG+TGG	+	contig477end:8193-8212	MsG0080048838.01.T01:CDS	40.0%
	ATTCCTCCCCAAAATAGTGA+TGG	-	contig477end:8155-8174	None:intergenic	40.0%
	GAGCATATCCTCTAGCAAAT+CGG	-	contig477end:8231-8250	None:intergenic	40.0%
	GGAGAAAAAGGATGGAGAAT+TGG	-	contig477end:7760-7779	None:intergenic	40.0%
	GTTAACTAAACCAAAGGCAG+TGG	-	contig477end:7981-8000	None:intergenic	40.0%
!	ATGCCTATAATTGGTGGTAG+TGG	+	contig477end:8199-8218	MsG0080048838.01.T01:CDS	40.0%
!	GGTAGCACCATCACTATTTT+GGG	+	contig477end:8145-8164	MsG0080048838.01.T01:CDS	40.0%
!	GTAGCACCATCACTATTTTG+GGG	+	contig477end:8146-8165	MsG0080048838.01.T01:CDS	40.0%
!	TGGTAGCACCATCACTATTT+TGG	+	contig477end:8144-8163	MsG0080048838.01.T01:CDS	40.0%
!!	GCAGTGGTTGAGTTTTTCAA+AGG	-	contig477end:7965-7984	None:intergenic	40.0%
!!	GTGGTTGAGTTTTTCAAAGG+TGG	-	contig477end:7962-7981	None:intergenic	40.0%
!!	GTTGAGTTTTTCAAAGGTGG+TGG	-	contig477end:7959-7978	None:intergenic	40.0%
!!	TTCAAAGGTGGTGGAACAAT+TGG	-	contig477end:7950-7969	None:intergenic	40.0%
	ATGTAAAGGGAGTGGTGAAG+AGG	-	contig477end:7781-7800	None:intergenic	45.0%
	GAGTGGTGAAGAGGAGAAAA+AGG	-	contig477end:7772-7791	None:intergenic	45.0%
	GGAACTCAATTCAGGTCCTA+AGG	-	contig477end:8023-8042	None:intergenic	45.0%
	GGTGAAGAGGAGAAAAAGGA+TGG	-	contig477end:7768-7787	None:intergenic	45.0%
!!!	GGTCTTTTCCGATTTGCTAG+AGG	+	contig477end:8220-8239	MsG0080048838.01.T01:CDS	45.0%
!!	GCACCATCACTATTTTGGGG+AGG	+	contig477end:8149-8168	MsG0080048838.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
contig477end	gene	7749	8327	7749	ID=MsG0080048838.01;Name=MsG0080048838.01
contig477end	mRNA	7749	8327	7749	ID=MsG0080048838.01.T01;Parent=MsG0080048838.01;Name=MsG0080048838.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|192
contig477end	exon	7749	8327	7749	ID=MsG0080048838.01.T01:exon:10846;Parent=MsG0080048838.01.T01
contig477end	CDS	7749	8327	7749	ID=MsG0080048838.01.T01:cds;Parent=MsG0080048838.01.T01

Gene Sequence

>MsG0080048838.01.T01

ATGTTCATCCAATTCTCCATCCTTTTTCTCCTCTTCACCACTCCCTTTACATCTTTAATCAAAGCAAAACAAGATGATGCCAATTTCGTACGTTCATTAGACCTCAAAGAATTAAATTTGGAGAATAAACAAGAGAAGCTAATCCATTTAAAATTCTATTGGCATGACATAGTTAGTGGAAACAACCCTTCTTCAATACCAATTGTTCCACCACCTTTGAAAAACTCAACCACTGCCTTTGGTTTAGTTAACATGATTGAGAACCCTTTAACCTTAGGACCTGAATTGAGTTCCAAATTGGTTGGAAAAGCACAAGGGTTCTATGCCTCAACATCACAAAGTGAGATAGACCTTATCATGGCTATGAATTTTGCTATCATTGAAGGAAAGTACAATGGTAGCACCATCACTATTTTGGGGAGGAATCCTGTTTATGATAAGGTTAGGGAAATGCCTATAATTGGTGGTAGTGGTCTTTTCCGATTTGCTAGAGGATATGCTCAACTTAGAACTCATTGGTTTTCATCCAAGACATATGATGCTATAGTTGAGTATAATATTTATGTGTTACATTATTAA

Protein sequence

>MsG0080048838.01.T01

MFIQFSILFLLFTTPFTSLIKAKQDDANFVRSLDLKELNLENKQEKLIHLKFYWHDIVSGNNPSSIPIVPPPLKNSTTAFGLVNMIENPLTLGPELSSKLVGKAQGFYASTSQSEIDLIMAMNFAIIEGKYNGSTITILGRNPVYDKVREMPIIGGSGLFRFARGYAQLRTHWFSSKTYDAIVEYNIYVLHY*