Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021436.01.T01 | XP_013456495.1 | 99.083 | 109 | 1 | 0 | 1 | 109 | 84 | 192 | 1.65E-74 | 229 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021436.01.T01 | Q84TH6 | 71.028 | 107 | 31 | 0 | 2 | 108 | 81 | 187 | 6.37E-57 | 177 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021436.01.T01 | A0A072UMB1 | 99.083 | 109 | 1 | 0 | 1 | 109 | 84 | 192 | 7.87e-75 | 229 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048509.01 | MsG0480021436.01 | 0.811803 | 5.831833e-51 | 3.644826e-48 |
| MsG0180000497.01 | MsG0480021436.01 | 0.828391 | 9.225547e-55 | 9.157889e-52 |
| MsG0180002808.01 | MsG0480021436.01 | 0.828594 | 8.244980e-55 | 8.232102e-52 |
| MsG0180003484.01 | MsG0480021436.01 | 0.813882 | 2.043599e-51 | 1.350730e-48 |
| MsG0180003504.01 | MsG0480021436.01 | 0.825094 | 5.653833e-54 | 5.103373e-51 |
| MsG0180004202.01 | MsG0480021436.01 | 0.807257 | 5.522782e-50 | 3.061666e-47 |
| MsG0180004467.01 | MsG0480021436.01 | 0.820405 | 6.978094e-53 | 5.516683e-50 |
| MsG0180005127.01 | MsG0480021436.01 | 0.807754 | 4.330494e-50 | 2.431973e-47 |
| MsG0180005762.01 | MsG0480021436.01 | 0.823534 | 1.315601e-53 | 1.135810e-50 |
| MsG0180006183.01 | MsG0480021436.01 | 0.860792 | 1.613254e-63 | 4.464150e-60 |
| MsG0180006253.01 | MsG0480021436.01 | 0.870809 | 1.101987e-66 | 4.344576e-63 |
| MsG0280006316.01 | MsG0480021436.01 | 0.817259 | 3.617422e-52 | 2.621432e-49 |
| MsG0280010617.01 | MsG0480021436.01 | 0.893307 | 7.097488e-75 | 6.725114e-71 |
| MsG0280010825.01 | MsG0480021436.01 | 0.813151 | 2.959616e-51 | 1.917600e-48 |
| MsG0280011016.01 | MsG0480021436.01 | 0.813675 | 2.269763e-51 | 1.491846e-48 |
| MsG0280011271.01 | MsG0480021436.01 | 0.815784 | 7.741520e-52 | 5.388614e-49 |
| MsG0380011686.01 | MsG0480021436.01 | 0.821629 | 3.647602e-53 | 2.983361e-50 |
| MsG0380012222.01 | MsG0480021436.01 | 0.818266 | 2.143699e-52 | 1.596992e-49 |
| MsG0380012540.01 | MsG0480021436.01 | 0.814325 | 1.631733e-51 | 1.091545e-48 |
| MsG0380014542.01 | MsG0480021436.01 | 0.816739 | 4.734067e-52 | 3.382217e-49 |
| MsG0380014544.01 | MsG0480021436.01 | 0.822215 | 2.668413e-53 | 2.219262e-50 |
| MsG0380014734.01 | MsG0480021436.01 | 0.804287 | 2.322775e-49 | 1.192984e-46 |
| MsG0480019818.01 | MsG0480021436.01 | 0.832338 | 1.000815e-55 | 1.115191e-52 |
| MsG0480020937.01 | MsG0480021436.01 | 0.819537 | 1.102438e-52 | 8.507449e-50 |
| MsG0480021226.01 | MsG0480021436.01 | 0.819175 | 1.333314e-52 | 1.018529e-49 |
| MsG0480021231.01 | MsG0480021436.01 | 0.816779 | 4.638425e-52 | 3.317537e-49 |
| MsG0480021234.01 | MsG0480021436.01 | 0.805497 | 1.297316e-49 | 6.873781e-47 |
| MsG0480021436.01 | MsG0480021773.01 | 0.810845 | 9.412603e-51 | 5.736008e-48 |
| MsG0480021436.01 | MsG0480022242.01 | -0.813396 | 2.613995e-51 | 1.705194e-48 |
| MsG0480021436.01 | MsG0480022244.01 | -0.838834 | 2.272459e-57 | 3.080581e-54 |
| MsG0480021436.01 | MsG0480022833.01 | 0.804239 | 2.376969e-49 | 1.219250e-46 |
| MsG0480021436.01 | MsG0480023322.01 | 0.812103 | 5.017036e-51 | 3.161176e-48 |
| MsG0480021436.01 | MsG0480023575.01 | 0.821274 | 4.405766e-53 | 3.567796e-50 |
| MsG0480021436.01 | MsG0480023842.01 | 0.805938 | 1.048312e-49 | 5.617956e-47 |
| MsG0480021436.01 | MsG0480023858.01 | 0.817695 | 2.885857e-52 | 2.116500e-49 |
| MsG0480021436.01 | MsG0480023913.01 | 0.804135 | 2.497578e-49 | 1.277775e-46 |
| MsG0480021436.01 | MsG0480024019.01 | 0.824076 | 9.815591e-54 | 8.605119e-51 |
| MsG0480021436.01 | MsG0580024980.01 | 0.833550 | 5.000787e-56 | 5.778483e-53 |
| MsG0480021436.01 | MsG0580025007.01 | 0.805409 | 1.353956e-49 | 7.157743e-47 |
| MsG0480021436.01 | MsG0580025024.01 | 0.804194 | 2.428120e-49 | 1.244098e-46 |
| MsG0480021436.01 | MsG0580026311.01 | 0.803948 | 2.731497e-49 | 1.390852e-46 |
| MsG0480021436.01 | MsG0580029158.01 | 0.801348 | 9.392663e-49 | 4.475504e-46 |
| MsG0480021436.01 | MsG0580030141.01 | 0.863464 | 2.443730e-64 | 7.414069e-61 |
| MsG0480021436.01 | MsG0680031356.01 | 0.842546 | 2.421031e-58 | 3.683418e-55 |
| MsG0480021436.01 | MsG0780038647.01 | 0.808314 | 3.292088e-50 | 1.876260e-47 |
| MsG0480021436.01 | MsG0780038919.01 | 0.801444 | 8.978066e-49 | 4.288260e-46 |
| MsG0480021436.01 | MsG0780039536.01 | 0.807432 | 5.070430e-50 | 2.823663e-47 |
| MsG0480021436.01 | MsG0780039923.01 | 0.813219 | 2.859973e-51 | 1.856424e-48 |
| MsG0480021436.01 | MsG0780039925.01 | 0.811806 | 5.821889e-51 | 3.638918e-48 |
| MsG0480021436.01 | MsG0780040081.01 | 0.820557 | 6.442318e-53 | 5.114232e-50 |
| MsG0480021436.01 | MsG0780040459.01 | 0.826587 | 2.499996e-54 | 2.354361e-51 |
| MsG0480021436.01 | MsG0780040554.01 | 0.827124 | 1.860260e-54 | 1.779426e-51 |
| MsG0480021436.01 | MsG0780040658.01 | 0.811138 | 8.133895e-51 | 4.995276e-48 |
| MsG0480021436.01 | MsG0780040670.01 | 0.812889 | 3.378108e-51 | 2.173524e-48 |
| MsG0480021436.01 | MsG0780040716.01 | 0.802912 | 4.478275e-49 | 2.220390e-46 |
| MsG0480021436.01 | MsG0780040808.01 | 0.800394 | 1.471468e-48 | 6.843883e-46 |
| MsG0480021436.01 | MsG0780040977.01 | 0.806735 | 7.119299e-50 | 3.893737e-47 |
| MsG0480021436.01 | MsG0780040991.01 | 0.814454 | 1.528338e-51 | 1.025945e-48 |
| MsG0480021436.01 | MsG0780041100.01 | 0.813385 | 2.628919e-51 | 1.714389e-48 |
| MsG0480021436.01 | MsG0780041447.01 | 0.819890 | 9.156392e-53 | 7.135876e-50 |
| MsG0480021436.01 | MsG0780041823.01 | 0.800978 | 1.118218e-48 | 5.278971e-46 |
| MsG0480021436.01 | MsG0880042392.01 | 0.819311 | 1.241239e-52 | 9.517258e-50 |
| MsG0480021436.01 | MsG0880042633.01 | 0.814799 | 1.281366e-51 | 8.682169e-49 |
| MsG0480021436.01 | MsG0880042635.01 | 0.814820 | 1.268078e-51 | 8.596716e-49 |
| MsG0480021436.01 | MsG0880043550.01 | 0.812001 | 5.280277e-51 | 3.317843e-48 |
| MsG0480021436.01 | MsG0880044834.01 | -0.813064 | 3.092426e-51 | 1.998981e-48 |
| MsG0480021436.01 | MsG0880045686.01 | 0.803602 | 3.223093e-49 | 1.626578e-46 |
| MsG0480021436.01 | MsG0880045802.01 | 0.842269 | 2.865938e-58 | 4.322429e-55 |
| MsG0480021436.01 | MsG0880046402.01 | 0.817678 | 2.911396e-52 | 2.134221e-49 |
| MsG0480021436.01 | MsG0880046403.01 | 0.808006 | 3.828369e-50 | 2.164211e-47 |
| MsG0480021436.01 | MsG0880046923.01 | 0.821601 | 3.703093e-53 | 3.026290e-50 |
| MsG0480021436.01 | MsG0880046992.01 | 0.823326 | 1.471283e-53 | 1.262745e-50 |
| MsG0480021436.01 | MsG0880047399.01 | -0.819698 | 1.012944e-52 | 7.851730e-50 |
| MsG0480021436.01 | MsG0880047414.01 | 0.800996 | 1.108993e-48 | 5.237788e-46 |
| MsG0480021436.01 | MsG0880047450.01 | 0.856561 | 2.959740e-62 | 7.093746e-59 |
| MsG0480021436.01 | MsG0880047664.01 | 0.801752 | 7.764505e-49 | 3.737426e-46 |
| MsG0380016250.01 | MsG0480021436.01 | 0.819390 | 1.191340e-52 | 9.154780e-50 |
| MsG0380016819.01 | MsG0480021436.01 | 0.857064 | 2.105443e-62 | 5.131292e-59 |
| MsG0380017621.01 | MsG0480021436.01 | 0.806326 | 8.686298e-50 | 4.701243e-47 |
| MsG0380017901.01 | MsG0480021436.01 | 0.804818 | 1.799442e-49 | 9.370221e-47 |
| MsG0480018228.01 | MsG0480021436.01 | 0.804966 | 1.676259e-49 | 8.761744e-47 |
| MsG0480018362.01 | MsG0480021436.01 | 0.874064 | 9.043130e-68 | 4.014109e-64 |
| MsG0480018439.01 | MsG0480021436.01 | 0.801011 | 1.100958e-48 | 5.201849e-46 |
| MsG0280006851.01 | MsG0480021436.01 | 0.850388 | 1.754040e-60 | 3.430252e-57 |
| MsG0280007423.01 | MsG0480021436.01 | -0.802420 | 5.657700e-49 | 2.770320e-46 |
| MsG0280007689.01 | MsG0480021436.01 | 0.837984 | 3.764967e-57 | 4.973898e-54 |
| MsG0280008226.01 | MsG0480021436.01 | 0.851610 | 7.932390e-61 | 1.614360e-57 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021436.01.T01 | MTR_4g073950 | 99.083 | 109 | 1 | 0 | 1 | 109 | 84 | 192 | 2.00e-78 | 229 |
| MsG0480021436.01.T01 | MTR_4g013350 | 63.889 | 108 | 39 | 0 | 2 | 109 | 90 | 197 | 1.19e-48 | 154 |
| MsG0480021436.01.T01 | MTR_4g013330 | 60.185 | 108 | 43 | 0 | 2 | 109 | 92 | 199 | 1.26e-47 | 151 |
| MsG0480021436.01.T01 | MTR_4g013345 | 62.037 | 108 | 41 | 0 | 2 | 109 | 89 | 196 | 3.31e-46 | 147 |
| MsG0480021436.01.T01 | MTR_4g013320 | 57.407 | 108 | 46 | 0 | 2 | 109 | 92 | 199 | 4.23e-44 | 142 |
| MsG0480021436.01.T01 | MTR_4g013325 | 56.481 | 108 | 47 | 0 | 2 | 109 | 92 | 199 | 1.65e-43 | 140 |
| MsG0480021436.01.T01 | MTR_7g070390 | 51.852 | 108 | 52 | 0 | 2 | 109 | 85 | 192 | 8.65e-41 | 133 |
| MsG0480021436.01.T01 | MTR_4g013335 | 53.704 | 108 | 50 | 0 | 2 | 109 | 89 | 196 | 5.58e-40 | 131 |
| MsG0480021436.01.T01 | MTR_1g054525 | 55.963 | 109 | 46 | 1 | 1 | 109 | 86 | 192 | 8.93e-39 | 128 |
| MsG0480021436.01.T01 | MTR_7g093830 | 55.140 | 107 | 47 | 1 | 4 | 109 | 87 | 193 | 1.01e-37 | 125 |
| MsG0480021436.01.T01 | MTR_7g093820 | 56.075 | 107 | 46 | 1 | 4 | 109 | 88 | 194 | 1.82e-37 | 125 |
| MsG0480021436.01.T01 | MTR_7g093850 | 54.206 | 107 | 48 | 1 | 4 | 109 | 88 | 194 | 7.16e-37 | 124 |
| MsG0480021436.01.T01 | MTR_4g013310 | 51.887 | 106 | 49 | 1 | 4 | 109 | 94 | 197 | 1.51e-34 | 118 |
| MsG0480021436.01.T01 | MTR_4g013385 | 50.000 | 106 | 53 | 0 | 4 | 109 | 85 | 190 | 3.56e-34 | 117 |
| MsG0480021436.01.T01 | MTR_0433s0040 | 50.000 | 106 | 53 | 0 | 4 | 109 | 85 | 190 | 3.56e-34 | 117 |
| MsG0480021436.01.T01 | MTR_4g013770 | 49.074 | 108 | 55 | 0 | 2 | 109 | 84 | 191 | 3.70e-34 | 117 |
| MsG0480021436.01.T01 | MTR_3g105640 | 49.533 | 107 | 53 | 1 | 4 | 109 | 82 | 188 | 6.17e-34 | 116 |
| MsG0480021436.01.T01 | MTR_7g093790 | 50.467 | 107 | 52 | 1 | 4 | 109 | 85 | 191 | 1.06e-33 | 115 |
| MsG0480021436.01.T01 | MTR_7g093870 | 46.429 | 112 | 57 | 1 | 1 | 109 | 82 | 193 | 5.30e-33 | 114 |
| MsG0480021436.01.T01 | MTR_1g056370 | 47.222 | 108 | 56 | 1 | 2 | 108 | 78 | 185 | 9.43e-33 | 113 |
| MsG0480021436.01.T01 | MTR_5g096120 | 48.598 | 107 | 54 | 1 | 4 | 109 | 83 | 189 | 2.21e-32 | 112 |
| MsG0480021436.01.T01 | MTR_4g078885 | 43.396 | 106 | 60 | 0 | 4 | 109 | 80 | 185 | 6.36e-29 | 103 |
| MsG0480021436.01.T01 | MTR_3g105630 | 43.925 | 107 | 59 | 1 | 4 | 109 | 83 | 189 | 1.82e-27 | 99.8 |
| MsG0480021436.01.T01 | MTR_4g013355 | 48.148 | 108 | 46 | 1 | 2 | 109 | 82 | 179 | 3.85e-27 | 98.6 |
| MsG0480021436.01.T01 | MTR_3g034030 | 41.509 | 106 | 62 | 0 | 4 | 109 | 80 | 185 | 3.97e-27 | 98.6 |
| MsG0480021436.01.T01 | MTR_4g122110 | 40.741 | 108 | 64 | 0 | 2 | 109 | 73 | 180 | 2.80e-21 | 83.6 |
| MsG0480021436.01.T01 | MTR_4g122130 | 37.963 | 108 | 67 | 0 | 2 | 109 | 73 | 180 | 7.60e-19 | 77.4 |
| MsG0480021436.01.T01 | MTR_4g049550 | 34.906 | 106 | 69 | 0 | 1 | 106 | 70 | 175 | 6.25e-15 | 67.0 |
| MsG0480021436.01.T01 | MTR_4g049570 | 33.019 | 106 | 71 | 0 | 1 | 106 | 70 | 175 | 2.49e-14 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021436.01.T01 | AT2G21100 | 71.028 | 107 | 31 | 0 | 2 | 108 | 81 | 187 | 6.50e-58 | 177 |
| MsG0480021436.01.T01 | AT2G21100 | 71.028 | 107 | 31 | 0 | 2 | 108 | 81 | 187 | 6.50e-58 | 177 |
| MsG0480021436.01.T01 | AT1G58170 | 55.556 | 108 | 48 | 0 | 2 | 109 | 78 | 185 | 2.52e-41 | 135 |
| MsG0480021436.01.T01 | AT1G55210 | 49.074 | 108 | 55 | 0 | 2 | 109 | 80 | 187 | 1.80e-36 | 122 |
| MsG0480021436.01.T01 | AT1G55210 | 49.074 | 108 | 55 | 0 | 2 | 109 | 80 | 187 | 1.80e-36 | 122 |
| MsG0480021436.01.T01 | AT3G13660 | 49.074 | 108 | 55 | 0 | 2 | 109 | 18 | 125 | 1.90e-36 | 120 |
| MsG0480021436.01.T01 | AT5G42510 | 53.704 | 108 | 48 | 1 | 1 | 108 | 77 | 182 | 7.05e-35 | 118 |
| MsG0480021436.01.T01 | AT5G49040 | 50.926 | 108 | 53 | 0 | 2 | 109 | 84 | 191 | 1.53e-34 | 117 |
| MsG0480021436.01.T01 | AT1G65870 | 51.402 | 107 | 52 | 0 | 2 | 108 | 83 | 189 | 4.64e-34 | 116 |
| MsG0480021436.01.T01 | AT5G42500 | 52.778 | 108 | 49 | 1 | 1 | 108 | 80 | 185 | 7.74e-34 | 115 |
| MsG0480021436.01.T01 | AT3G13650 | 49.057 | 106 | 54 | 0 | 4 | 109 | 81 | 186 | 1.38e-33 | 115 |
| MsG0480021436.01.T01 | AT3G58090 | 49.533 | 107 | 54 | 0 | 2 | 108 | 165 | 271 | 2.28e-31 | 112 |
| MsG0480021436.01.T01 | AT3G13662 | 50.526 | 95 | 47 | 0 | 2 | 96 | 78 | 172 | 6.14e-30 | 105 |
| MsG0480021436.01.T01 | AT2G21110 | 49.057 | 106 | 54 | 0 | 4 | 109 | 81 | 186 | 7.47e-30 | 105 |
| MsG0480021436.01.T01 | AT1G22900 | 47.222 | 108 | 55 | 1 | 1 | 108 | 88 | 193 | 1.03e-29 | 105 |
| MsG0480021436.01.T01 | AT5G49030 | 51.064 | 94 | 46 | 0 | 2 | 95 | 84 | 177 | 6.32e-26 | 100 |
| MsG0480021436.01.T01 | AT4G38700 | 41.818 | 110 | 60 | 2 | 4 | 109 | 81 | 190 | 1.14e-25 | 95.1 |
| MsG0480021436.01.T01 | AT4G11210 | 35.870 | 92 | 58 | 1 | 1 | 92 | 76 | 166 | 8.57e-13 | 61.6 |
Find 33 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGCCCACGAGCTTGGATTT+TGG | 0.153982 | 4:-57854386 | None:intergenic |
| GGATCAGATGTTTCAGTTAA+AGG | 0.192736 | 4:-57854365 | None:intergenic |
| GGTAGCACATTTGCTCTTGT+TGG | 0.341517 | 4:+57854497 | MsG0480021436.01.T01:CDS |
| ATGCCTGTTGTTGGAGGTAC+TGG | 0.347518 | 4:+57854551 | MsG0480021436.01.T01:CDS |
| ATGCCATTGCTAAGACTCAT+TGG | 0.400986 | 4:+57854597 | MsG0480021436.01.T01:CDS |
| TTTGTGGATGGGCCGTATAA+TGG | 0.409954 | 4:+57854476 | MsG0480021436.01.T01:CDS |
| GCAAATGTGCTACCATTATA+CGG | 0.416384 | 4:-57854488 | None:intergenic |
| TTGAGCTACTCCTTTGTGGA+TGG | 0.418314 | 4:+57854464 | MsG0480021436.01.T01:CDS |
| TCATCGTGTCAGCAGGAGAT+TGG | 0.421466 | 4:+57854428 | MsG0480021436.01.T01:CDS |
| GTCGTTGAGCTACTCCTTTG+TGG | 0.422168 | 4:+57854460 | MsG0480021436.01.T01:CDS |
| ACAACAGGCATCTCACGAAC+CGG | 0.432988 | 4:-57854539 | None:intergenic |
| TTGTGCTCGGCCCACGAGCT+TGG | 0.436650 | 4:-57854393 | None:intergenic |
| CATCGTGTCAGCAGGAGATT+GGG | 0.452951 | 4:+57854429 | MsG0480021436.01.T01:CDS |
| GTTCGTGAGATGCCTGTTGT+TGG | 0.459287 | 4:+57854542 | MsG0480021436.01.T01:CDS |
| CATTGGATTGATATTACTAC+TGG | 0.460050 | 4:+57854614 | MsG0480021436.01.T01:CDS |
| ACTACTGGTGATGCTATTGT+TGG | 0.469577 | 4:+57854629 | MsG0480021436.01.T01:CDS |
| TCACGAACCGGGTTCATAGC+AGG | 0.470658 | 4:-57854527 | None:intergenic |
| TGAGCTACTCCTTTGTGGAT+GGG | 0.497718 | 4:+57854465 | MsG0480021436.01.T01:CDS |
| AGTCCAGTACCTCCAACAAC+AGG | 0.500575 | 4:-57854554 | None:intergenic |
| TTACGCGTCATCGTGTCAGC+AGG | 0.513526 | 4:+57854421 | MsG0480021436.01.T01:CDS |
| CGTGAGATGCCTGTTGTTGG+AGG | 0.525744 | 4:+57854545 | MsG0480021436.01.T01:CDS |
| TGGCATAACCCCTTGCCATA+CGG | 0.526929 | 4:-57854580 | None:intergenic |
| AAGCTCGTGGGCCGAGCACA+AGG | 0.551866 | 4:+57854395 | MsG0480021436.01.T01:CDS |
| CACGAACCGGGTTCATAGCA+GGG | 0.575135 | 4:-57854526 | None:intergenic |
| TGATCCAAAATCCAAGCTCG+TGG | 0.590503 | 4:+57854382 | MsG0480021436.01.T01:CDS |
| CAACAGGCATCTCACGAACC+GGG | 0.593590 | 4:-57854538 | None:intergenic |
| ACGCGTAAATACCTTGTGCT+CGG | 0.608058 | 4:-57854406 | None:intergenic |
| AAAGAACCCTGCTATGAACC+CGG | 0.622651 | 4:+57854520 | MsG0480021436.01.T01:CDS |
| GATCCAAAATCCAAGCTCGT+GGG | 0.650480 | 4:+57854383 | MsG0480021436.01.T01:CDS |
| AATCCAATGAGTCTTAGCAA+TGG | 0.659713 | 4:-57854600 | None:intergenic |
| ATGCTATTAATAATGAACGA+GGG | 0.668500 | 4:-57854663 | None:intergenic |
| ATTATACGGCCCATCCACAA+AGG | 0.683112 | 4:-57854474 | None:intergenic |
| AATGCTATTAATAATGAACG+AGG | 0.719484 | 4:-57854664 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CATTGGATTGATATTACTAC+TGG | + | Chr4:57854614-57854633 | MsG0480021436.01.T01:CDS | 30.0% | |
| AATCCAATGAGTCTTAGCAA+TGG | - | Chr4:57854603-57854622 | None:intergenic | 35.0% | |
| GCAAATGTGCTACCATTATA+CGG | - | Chr4:57854491-57854510 | None:intergenic | 35.0% | |
| GGATCAGATGTTTCAGTTAA+AGG | - | Chr4:57854368-57854387 | None:intergenic | 35.0% | |
| ATGCCATTGCTAAGACTCAT+TGG | + | Chr4:57854597-57854616 | MsG0480021436.01.T01:CDS | 40.0% | |
| ! | ACTACTGGTGATGCTATTGT+TGG | + | Chr4:57854629-57854648 | MsG0480021436.01.T01:CDS | 40.0% |
| AAAGAACCCTGCTATGAACC+CGG | + | Chr4:57854520-57854539 | MsG0480021436.01.T01:CDS | 45.0% | |
| ACGCGTAAATACCTTGTGCT+CGG | - | Chr4:57854409-57854428 | None:intergenic | 45.0% | |
| ATTATACGGCCCATCCACAA+AGG | - | Chr4:57854477-57854496 | None:intergenic | 45.0% | |
| GATCCAAAATCCAAGCTCGT+GGG | + | Chr4:57854383-57854402 | MsG0480021436.01.T01:CDS | 45.0% | |
| GGTAGCACATTTGCTCTTGT+TGG | + | Chr4:57854497-57854516 | MsG0480021436.01.T01:CDS | 45.0% | |
| TGAGCTACTCCTTTGTGGAT+GGG | + | Chr4:57854465-57854484 | MsG0480021436.01.T01:CDS | 45.0% | |
| TGATCCAAAATCCAAGCTCG+TGG | + | Chr4:57854382-57854401 | MsG0480021436.01.T01:CDS | 45.0% | |
| TTGAGCTACTCCTTTGTGGA+TGG | + | Chr4:57854464-57854483 | MsG0480021436.01.T01:CDS | 45.0% | |
| TTTGTGGATGGGCCGTATAA+TGG | + | Chr4:57854476-57854495 | MsG0480021436.01.T01:CDS | 45.0% | |
| ! | TGGACTTTTCCGTATGGCAA+GGG | + | Chr4:57854571-57854590 | MsG0480021436.01.T01:CDS | 45.0% |
| !!! | AGGTACTGGACTTTTCCGTA+TGG | + | Chr4:57854565-57854584 | MsG0480021436.01.T01:CDS | 45.0% |
| ACAACAGGCATCTCACGAAC+CGG | - | Chr4:57854542-57854561 | None:intergenic | 50.0% | |
| AGTCCAGTACCTCCAACAAC+AGG | - | Chr4:57854557-57854576 | None:intergenic | 50.0% | |
| CATCGTGTCAGCAGGAGATT+GGG | + | Chr4:57854429-57854448 | MsG0480021436.01.T01:CDS | 50.0% | |
| GTCGTTGAGCTACTCCTTTG+TGG | + | Chr4:57854460-57854479 | MsG0480021436.01.T01:CDS | 50.0% | |
| GTTCGTGAGATGCCTGTTGT+TGG | + | Chr4:57854542-57854561 | MsG0480021436.01.T01:CDS | 50.0% | |
| TCATCGTGTCAGCAGGAGAT+TGG | + | Chr4:57854428-57854447 | MsG0480021436.01.T01:CDS | 50.0% | |
| TGGCATAACCCCTTGCCATA+CGG | - | Chr4:57854583-57854602 | None:intergenic | 50.0% | |
| ! | ATGCCTGTTGTTGGAGGTAC+TGG | + | Chr4:57854551-57854570 | MsG0480021436.01.T01:CDS | 50.0% |
| ! | CTGGACTTTTCCGTATGGCA+AGG | + | Chr4:57854570-57854589 | MsG0480021436.01.T01:CDS | 50.0% |
| ! | GGACTTTTCCGTATGGCAAG+GGG | + | Chr4:57854572-57854591 | MsG0480021436.01.T01:CDS | 50.0% |
| CAACAGGCATCTCACGAACC+GGG | - | Chr4:57854541-57854560 | None:intergenic | 55.0% | |
| CACGAACCGGGTTCATAGCA+GGG | - | Chr4:57854529-57854548 | None:intergenic | 55.0% | |
| CGTGAGATGCCTGTTGTTGG+AGG | + | Chr4:57854545-57854564 | MsG0480021436.01.T01:CDS | 55.0% | |
| TCACGAACCGGGTTCATAGC+AGG | - | Chr4:57854530-57854549 | None:intergenic | 55.0% | |
| TTACGCGTCATCGTGTCAGC+AGG | + | Chr4:57854421-57854440 | MsG0480021436.01.T01:CDS | 55.0% | |
| ! | CGGCCCACGAGCTTGGATTT+TGG | - | Chr4:57854389-57854408 | None:intergenic | 60.0% |
| AAGCTCGTGGGCCGAGCACA+AGG | + | Chr4:57854395-57854414 | MsG0480021436.01.T01:CDS | 65.0% | |
| TTGTGCTCGGCCCACGAGCT+TGG | - | Chr4:57854396-57854415 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 57854350 | 57854679 | 57854350 | ID=MsG0480021436.01;Name=MsG0480021436.01 |
| Chr4 | mRNA | 57854350 | 57854679 | 57854350 | ID=MsG0480021436.01.T01;Parent=MsG0480021436.01;Name=MsG0480021436.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|109 |
| Chr4 | exon | 57854350 | 57854679 | 57854350 | ID=MsG0480021436.01.T01:exon:6646;Parent=MsG0480021436.01.T01 |
| Chr4 | CDS | 57854350 | 57854679 | 57854350 | ID=MsG0480021436.01.T01:cds;Parent=MsG0480021436.01.T01 |
| Gene Sequence |
| Protein sequence |
