AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040609.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040609.01.T01 MTR_7g093790 95.620 137 6 0 1 137 55 191 7.38e-95 271
MsG0780040609.01.T01 MTR_7g093820 82.222 135 24 0 3 137 60 194 1.18e-80 236
MsG0780040609.01.T01 MTR_7g093850 81.481 135 25 0 3 137 60 194 3.28e-80 234
MsG0780040609.01.T01 MTR_7g093830 79.259 135 28 0 3 137 59 193 2.43e-78 230
MsG0780040609.01.T01 MTR_7g093870 57.042 142 55 3 1 137 53 193 1.61e-52 165
MsG0780040609.01.T01 MTR_4g013350 54.745 137 61 1 1 137 62 197 1.16e-50 160
MsG0780040609.01.T01 MTR_4g013320 53.285 137 63 1 1 137 64 199 7.67e-49 155
MsG0780040609.01.T01 MTR_4g013325 51.095 137 66 1 1 137 64 199 6.86e-48 153
MsG0780040609.01.T01 MTR_4g013345 52.555 137 64 1 1 137 61 196 1.07e-47 152
MsG0780040609.01.T01 MTR_4g013335 50.365 137 65 2 1 137 63 196 1.50e-47 152
MsG0780040609.01.T01 MTR_4g013310 52.555 137 62 2 1 137 64 197 2.37e-45 146
MsG0780040609.01.T01 MTR_4g013330 49.640 139 67 2 1 137 62 199 2.96e-45 146
MsG0780040609.01.T01 MTR_1g054525 54.074 135 59 2 3 137 61 192 8.63e-45 145
MsG0780040609.01.T01 MTR_7g070390 57.895 114 47 1 24 137 80 192 2.24e-43 141
MsG0780040609.01.T01 MTR_4g013770 46.324 136 71 2 3 137 57 191 5.28e-41 135
MsG0780040609.01.T01 MTR_4g073950 48.551 138 69 2 1 137 56 192 3.32e-40 133
MsG0780040609.01.T01 MTR_4g013385 47.445 137 70 2 1 137 56 190 6.90e-40 132
MsG0780040609.01.T01 MTR_0433s0040 47.445 137 70 2 1 137 56 190 6.90e-40 132
MsG0780040609.01.T01 MTR_3g105640 48.201 139 69 3 1 137 51 188 6.79e-39 130
MsG0780040609.01.T01 MTR_3g105630 43.571 140 75 3 1 137 51 189 1.22e-36 124
MsG0780040609.01.T01 MTR_5g096120 47.518 141 65 4 3 137 52 189 2.74e-35 120
MsG0780040609.01.T01 MTR_4g013355 50.000 114 46 1 24 137 77 179 7.07e-35 119
MsG0780040609.01.T01 MTR_1g056370 43.066 137 75 3 1 136 51 185 1.52e-33 116
MsG0780040609.01.T01 MTR_4g078885 46.400 125 57 4 14 137 70 185 1.72e-30 108
MsG0780040609.01.T01 MTR_3g034030 46.400 125 57 4 14 137 70 185 8.08e-30 106
MsG0780040609.01.T01 MTR_4g122130 42.963 135 76 1 3 137 47 180 3.70e-28 102
MsG0780040609.01.T01 MTR_4g122110 40.741 135 79 1 3 137 47 180 1.70e-27 100
MsG0780040609.01.T01 MTR_4g049550 34.746 118 76 1 15 132 57 173 8.42e-18 75.5
MsG0780040609.01.T01 MTR_4g049570 37.273 110 68 1 2 111 44 152 1.03e-17 75.5
MsG0780040609.01.T01 MTR_1g046490 42.553 94 52 2 22 115 120 211 2.33e-15 70.5
MsG0780040609.01.T01 MTR_1g046800 43.617 94 51 2 22 115 134 225 2.36e-15 70.5
MsG0780040609.01.T01 MTR_1g046500 43.617 94 51 2 22 115 134 225 3.87e-15 70.1
MsG0780040609.01.T01 MTR_4g062520 36.449 107 67 1 14 120 177 282 1.59e-11 60.5
MsG0780040609.01.T01 MTR_4g062470 36.449 107 67 1 14 120 178 283 2.08e-11 60.1
MsG0780040609.01.T01 MTR_1g115510 32.558 129 81 4 14 136 225 353 5.07e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040609.01.T01 AT1G58170 45.926 135 72 1 3 137 52 185 1.77e-43 141
MsG0780040609.01.T01 AT5G42500 51.471 136 63 2 1 136 53 185 8.18e-40 132
MsG0780040609.01.T01 AT1G65870 47.826 138 67 3 1 136 55 189 4.43e-39 130
MsG0780040609.01.T01 AT3G58090 49.618 131 60 2 6 136 147 271 4.77e-39 132
MsG0780040609.01.T01 AT1G22900 50.000 136 65 2 1 136 61 193 2.82e-38 128
MsG0780040609.01.T01 AT1G55210 43.796 137 73 2 1 137 55 187 3.69e-38 128
MsG0780040609.01.T01 AT1G55210 43.796 137 73 2 1 137 55 187 3.69e-38 128
MsG0780040609.01.T01 AT5G42510 48.529 136 67 2 1 136 50 182 5.64e-38 127
MsG0780040609.01.T01 AT5G49040 42.222 135 77 1 3 137 58 191 7.65e-37 124
MsG0780040609.01.T01 AT3G13650 42.336 137 75 2 1 137 54 186 9.13e-36 122
MsG0780040609.01.T01 AT2G21110 39.716 141 80 2 1 137 47 186 1.65e-33 116
MsG0780040609.01.T01 AT3G13660 44.348 115 63 1 23 137 12 125 1.88e-32 111
MsG0780040609.01.T01 AT2G21100 43.382 136 76 1 1 136 53 187 3.26e-32 112
MsG0780040609.01.T01 AT2G21100 43.382 136 76 1 1 136 53 187 3.26e-32 112
MsG0780040609.01.T01 AT3G13662 42.742 124 67 2 1 124 53 172 1.80e-31 110
MsG0780040609.01.T01 AT4G38700 41.549 142 78 3 1 137 49 190 2.40e-31 110
MsG0780040609.01.T01 AT5G49030 44.248 113 62 1 3 115 58 169 7.81e-28 107
MsG0780040609.01.T01 AT3G24020 38.947 95 56 2 21 115 126 218 4.73e-12 61.6
MsG0780040609.01.T01 AT2G28670 37.273 110 67 1 14 123 95 202 1.26e-11 60.1
MsG0780040609.01.T01 AT4G13580 37.895 95 57 2 21 115 127 219 1.30e-11 60.5
MsG0780040609.01.T01 AT2G28670 37.273 110 67 1 14 123 305 412 4.79e-11 59.3

Find 40 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTATCACTGCGCTTGCTTT+GGG 0.227737 7:+79917146 None:intergenic
TACTATCACTGCGCTTGCTT+TGG 0.236778 7:+79917145 None:intergenic
ACAATAGGCATTTCTCTAAT+AGG 0.277388 7:+79916882 None:intergenic
TTCTCAGAGGGAGAATTTAA+TGG 0.312603 7:-79916948 MsG0780040609.01.T01:CDS
TGGAAGCACACTTAGTATAT+TGG 0.330019 7:-79916928 MsG0780040609.01.T01:CDS
ATGCCTATTGTTGGTGGAAC+TGG 0.330790 7:-79916870 MsG0780040609.01.T01:CDS
TTGAAAATTGTCTTCCATTC+GGG 0.334163 7:-79917103 MsG0780040609.01.T01:CDS
TAATGCTAATTAATTAATAA+TGG 0.335277 7:+79916751 None:intergenic
GTTGAAAATTGTCTTCCATT+CGG 0.400576 7:-79917104 MsG0780040609.01.T01:CDS
TTCAACCTTGCCGTTGGGCT+CGG 0.423251 7:+79917121 None:intergenic
ATCGATGATCCGTTGACGAT+TGG 0.430642 7:-79917068 MsG0780040609.01.T01:CDS
TCGATGATCCGTTGACGATT+GGG 0.450334 7:-79917067 MsG0780040609.01.T01:CDS
ATTAGAGAAATGCCTATTGT+TGG 0.451563 7:-79916879 MsG0780040609.01.T01:CDS
TGCCTATTGTTGGTGGAACT+GGG 0.470280 7:-79916869 MsG0780040609.01.T01:CDS
TTCAATATCACAAGATGAGA+TGG 0.473863 7:-79916997 MsG0780040609.01.T01:CDS
ACGAGTTTCGAGTCAAGTTC+AGG 0.480237 7:+79917044 None:intergenic
GCCCCAGTTCCACCAACAAT+AGG 0.482868 7:+79916867 None:intergenic
CTATCACTGCGCTTGCTTTG+GGG 0.493102 7:+79917147 None:intergenic
AGAGAAATGCCTATTGTTGG+TGG 0.501448 7:-79916876 MsG0780040609.01.T01:CDS
AGATGAGATGGGACTAATGA+TGG 0.517218 7:-79916985 MsG0780040609.01.T01:CDS
TGCCACTGGAAATGCTGTTG+TGG 0.551249 7:-79916793 MsG0780040609.01.T01:CDS
TATTTCCACGACGTTATGGA+AGG 0.579634 7:-79917170 None:intergenic
AAACTCGTGGGTAAAGCTCA+AGG 0.587917 7:-79917029 MsG0780040609.01.T01:CDS
TCTTCCATTCGGGACAGTAG+TGG 0.594605 7:-79917093 MsG0780040609.01.T01:CDS
ATGACATGGGCGTTCTCAGA+GGG 0.596194 7:-79916960 MsG0780040609.01.T01:CDS
AAAACTCACAGAGTAAAACT+TGG 0.604813 7:+79916815 None:intergenic
AATCGTCAACGGATCATCGA+TGG 0.604968 7:+79917070 None:intergenic
TCAATATCACAAGATGAGAT+GGG 0.609527 7:-79916996 MsG0780040609.01.T01:CDS
GCCTATTGTTGGTGGAACTG+GGG 0.613108 7:-79916868 MsG0780040609.01.T01:CDS
ATGGCCACTACTGTCCCGAA+TGG 0.621439 7:+79917089 None:intergenic
AGTAGCCGAGCCCAACGGCA+AGG 0.624037 7:-79917126 MsG0780040609.01.T01:CDS
AGTTCAGGCCCAATCGTCAA+CGG 0.624507 7:+79917059 None:intergenic
GTGATAGTAGCCGAGCCCAA+CGG 0.625680 7:-79917131 MsG0780040609.01.T01:CDS
GCACACTTAGTATATTGGCT+AGG 0.626552 7:-79916923 MsG0780040609.01.T01:CDS
TGAACTTGACTCGAAACTCG+TGG 0.629246 7:-79917042 MsG0780040609.01.T01:CDS
GAACTTGACTCGAAACTCGT+GGG 0.645746 7:-79917041 MsG0780040609.01.T01:CDS
GACTAATGATGGTTATGACA+TGG 0.654276 7:-79916974 MsG0780040609.01.T01:CDS
TTCCACAACAGCATTTCCAG+TGG 0.674988 7:+79916791 None:intergenic
TATGACATGGGCGTTCTCAG+AGG 0.685781 7:-79916961 MsG0780040609.01.T01:CDS
ACTAATGATGGTTATGACAT+GGG 0.689534 7:-79916973 MsG0780040609.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAACTCACAGAGTAAAACT+TGG + Chr7:79917107-79917126 None:intergenic 30.0%
ACAATAGGCATTTCTCTAAT+AGG + Chr7:79917040-79917059 None:intergenic 30.0%
ACTAATGATGGTTATGACAT+GGG - Chr7:79916946-79916965 MsG0780040609.01.T01:CDS 30.0%
ATTAGAGAAATGCCTATTGT+TGG - Chr7:79917040-79917059 MsG0780040609.01.T01:CDS 30.0%
GTTGAAAATTGTCTTCCATT+CGG - Chr7:79916815-79916834 MsG0780040609.01.T01:CDS 30.0%
TCAATATCACAAGATGAGAT+GGG - Chr7:79916923-79916942 MsG0780040609.01.T01:CDS 30.0%
TTCAATATCACAAGATGAGA+TGG - Chr7:79916922-79916941 MsG0780040609.01.T01:CDS 30.0%
TTGAAAATTGTCTTCCATTC+GGG - Chr7:79916816-79916835 MsG0780040609.01.T01:CDS 30.0%
GACTAATGATGGTTATGACA+TGG - Chr7:79916945-79916964 MsG0780040609.01.T01:CDS 35.0%
TTCTCAGAGGGAGAATTTAA+TGG - Chr7:79916971-79916990 MsG0780040609.01.T01:CDS 35.0%
! TGGAAGCACACTTAGTATAT+TGG - Chr7:79916991-79917010 MsG0780040609.01.T01:CDS 35.0%
AGAGAAATGCCTATTGTTGG+TGG - Chr7:79917043-79917062 MsG0780040609.01.T01:CDS 40.0%
AGATGAGATGGGACTAATGA+TGG - Chr7:79916934-79916953 MsG0780040609.01.T01:CDS 40.0%
! ACAATTTTCAACCTTGCCGT+TGG + Chr7:79916807-79916826 None:intergenic 40.0%
! CAATTTTCAACCTTGCCGTT+GGG + Chr7:79916806-79916825 None:intergenic 40.0%
! GCACACTTAGTATATTGGCT+AGG - Chr7:79916996-79917015 MsG0780040609.01.T01:CDS 40.0%
AAACTCGTGGGTAAAGCTCA+AGG - Chr7:79916890-79916909 MsG0780040609.01.T01:CDS 45.0%
AATCGTCAACGGATCATCGA+TGG + Chr7:79916852-79916871 None:intergenic 45.0%
ACTATCACTGCGCTTGCTTT+GGG + Chr7:79916776-79916795 None:intergenic 45.0%
ATCGATGATCCGTTGACGAT+TGG - Chr7:79916851-79916870 MsG0780040609.01.T01:CDS 45.0%
TACTATCACTGCGCTTGCTT+TGG + Chr7:79916777-79916796 None:intergenic 45.0%
TCGATGATCCGTTGACGATT+GGG - Chr7:79916852-79916871 MsG0780040609.01.T01:CDS 45.0%
TTCCACAACAGCATTTCCAG+TGG + Chr7:79917131-79917150 None:intergenic 45.0%
! ACGAGTTTCGAGTCAAGTTC+AGG + Chr7:79916878-79916897 None:intergenic 45.0%
! ATGCCTATTGTTGGTGGAAC+TGG - Chr7:79917049-79917068 MsG0780040609.01.T01:CDS 45.0%
! GAACTTGACTCGAAACTCGT+GGG - Chr7:79916878-79916897 MsG0780040609.01.T01:CDS 45.0%
! GGGGCTTTTAGATTTGTACG+TGG - Chr7:79917070-79917089 MsG0780040609.01.T01:CDS 45.0%
! TACTCTGTGAGTTTTGCCAC+TGG - Chr7:79917112-79917131 MsG0780040609.01.T01:CDS 45.0%
! TGAACTTGACTCGAAACTCG+TGG - Chr7:79916877-79916896 MsG0780040609.01.T01:CDS 45.0%
! TGCCTATTGTTGGTGGAACT+GGG - Chr7:79917050-79917069 MsG0780040609.01.T01:CDS 45.0%
AGTTCAGGCCCAATCGTCAA+CGG + Chr7:79916863-79916882 None:intergenic 50.0%
ATGACATGGGCGTTCTCAGA+GGG - Chr7:79916959-79916978 MsG0780040609.01.T01:CDS 50.0%
CTATCACTGCGCTTGCTTTG+GGG + Chr7:79916775-79916794 None:intergenic 50.0%
TATGACATGGGCGTTCTCAG+AGG - Chr7:79916958-79916977 MsG0780040609.01.T01:CDS 50.0%
TCTTCCATTCGGGACAGTAG+TGG - Chr7:79916826-79916845 MsG0780040609.01.T01:CDS 50.0%
TGCCACTGGAAATGCTGTTG+TGG - Chr7:79917126-79917145 MsG0780040609.01.T01:CDS 50.0%
! GCCTATTGTTGGTGGAACTG+GGG - Chr7:79917051-79917070 MsG0780040609.01.T01:CDS 50.0%
ATGGCCACTACTGTCCCGAA+TGG + Chr7:79916833-79916852 None:intergenic 55.0%
GCCCCAGTTCCACCAACAAT+AGG + Chr7:79917055-79917074 None:intergenic 55.0%
GTGATAGTAGCCGAGCCCAA+CGG - Chr7:79916788-79916807 MsG0780040609.01.T01:CDS 55.0%
TTCAACCTTGCCGTTGGGCT+CGG + Chr7:79916801-79916820 None:intergenic 55.0%
AGTAGCCGAGCCCAACGGCA+AGG - Chr7:79916793-79916812 MsG0780040609.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 79916764 79917177 79916764 ID=MsG0780040609.01;Name=MsG0780040609.01
Chr7 mRNA 79916764 79917177 79916764 ID=MsG0780040609.01.T01;Parent=MsG0780040609.01;Name=MsG0780040609.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|137
Chr7 exon 79916764 79917177 79916764 ID=MsG0780040609.01.T01:exon:18500;Parent=MsG0780040609.01.T01
Chr7 CDS 79916764 79917177 79916764 ID=MsG0780040609.01.T01:cds;Parent=MsG0780040609.01.T01
Gene Sequence

>MsG0780040609.01.T01

ATGGAAGGCCCCAAAGCAAGCGCAGTGATAGTAGCCGAGCCCAACGGCAAGGTTGAAAATTGTCTTCCATTCGGGACAGTAGTGGCCATCGATGATCCGTTGACGATTGGGCCTGAACTTGACTCGAAACTCGTGGGTAAAGCTCAAGGAATTTACGCTTCAATATCACAAGATGAGATGGGACTAATGATGGTTATGACATGGGCGTTCTCAGAGGGAGAATTTAATGGAAGCACACTTAGTATATTGGCTAGGAACATGATCGTGAGTGAACCTATTAGAGAAATGCCTATTGTTGGTGGAACTGGGGCTTTTAGATTTGTACGTGGTTATGCTCAAGCCAAGTTTTACTCTGTGAGTTTTGCCACTGGAAATGCTGTTGTGGAATATGATGTATTCGTGTTCCATTATTAA

Protein sequence

>MsG0780040609.01.T01

MEGPKASAVIVAEPNGKVENCLPFGTVVAIDDPLTIGPELDSKLVGKAQGIYASISQDEMGLMMVMTWAFSEGEFNGSTLSILARNMIVSEPIREMPIVGGTGAFRFVRGYAQAKFYSVSFATGNAVVEYDVFVFHY*