AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040611.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040611.01.T01 MTR_7g093820 98.718 156 2 0 1 156 39 194 5.96e-115 323
MsG0780040611.01.T01 MTR_7g093850 97.436 156 4 0 1 156 39 194 1.83e-113 320
MsG0780040611.01.T01 MTR_7g093830 96.795 156 5 0 1 156 38 193 3.12e-112 317
MsG0780040611.01.T01 MTR_7g093790 80.769 156 30 0 1 156 36 191 7.36e-95 272
MsG0780040611.01.T01 MTR_7g093870 60.625 160 57 3 2 156 35 193 1.41e-65 199
MsG0780040611.01.T01 MTR_4g013350 52.229 157 73 2 1 156 42 197 4.38e-57 177
MsG0780040611.01.T01 MTR_4g013345 52.229 157 73 2 1 156 41 196 7.84e-56 174
MsG0780040611.01.T01 MTR_1g054525 54.605 152 66 2 5 156 44 192 2.04e-54 170
MsG0780040611.01.T01 MTR_4g013335 50.318 157 74 3 1 156 43 196 4.64e-54 169
MsG0780040611.01.T01 MTR_4g013330 50.314 159 75 3 1 156 42 199 1.43e-53 168
MsG0780040611.01.T01 MTR_4g013320 51.316 152 73 1 5 156 49 199 2.10e-53 168
MsG0780040611.01.T01 MTR_4g013325 52.740 146 68 1 11 156 55 199 5.02e-53 167
MsG0780040611.01.T01 MTR_4g073950 52.288 153 71 2 5 156 41 192 3.51e-51 162
MsG0780040611.01.T01 MTR_3g105640 52.941 153 69 3 6 156 37 188 4.59e-50 159
MsG0780040611.01.T01 MTR_4g013310 50.658 152 72 2 5 156 49 197 4.95e-50 159
MsG0780040611.01.T01 MTR_3g105630 50.000 154 73 3 6 156 37 189 3.68e-49 157
MsG0780040611.01.T01 MTR_7g070390 51.316 152 72 2 5 156 43 192 4.12e-47 151
MsG0780040611.01.T01 MTR_4g013385 45.395 152 81 2 5 156 41 190 2.12e-44 144
MsG0780040611.01.T01 MTR_0433s0040 45.395 152 81 2 5 156 41 190 2.12e-44 144
MsG0780040611.01.T01 MTR_5g096120 49.677 155 71 4 7 156 37 189 2.18e-44 144
MsG0780040611.01.T01 MTR_1g056370 47.619 147 74 3 10 155 41 185 1.62e-42 139
MsG0780040611.01.T01 MTR_4g013770 41.892 148 84 2 10 156 45 191 3.91e-41 136
MsG0780040611.01.T01 MTR_3g034030 46.358 151 76 4 7 156 39 185 6.68e-40 133
MsG0780040611.01.T01 MTR_4g078885 45.395 152 78 4 6 156 38 185 2.43e-39 131
MsG0780040611.01.T01 MTR_4g013355 39.474 152 81 1 5 156 39 179 7.86e-36 122
MsG0780040611.01.T01 MTR_4g122110 40.397 151 89 1 6 156 31 180 4.29e-30 108
MsG0780040611.01.T01 MTR_4g122130 40.397 151 89 1 6 156 31 180 1.09e-29 107
MsG0780040611.01.T01 MTR_4g049570 32.215 149 100 1 3 151 26 173 3.43e-21 85.1
MsG0780040611.01.T01 MTR_4g049550 30.822 146 100 1 6 151 29 173 2.64e-20 82.8
MsG0780040611.01.T01 MTR_1g046490 38.947 95 56 2 40 134 119 211 9.72e-14 66.6
MsG0780040611.01.T01 MTR_1g046800 40.000 95 55 2 40 134 133 225 1.01e-13 66.6
MsG0780040611.01.T01 MTR_1g046500 40.000 95 55 2 40 134 133 225 1.53e-13 66.2
MsG0780040611.01.T01 MTR_1g115510 36.154 130 75 5 33 155 225 353 9.08e-13 64.7
MsG0780040611.01.T01 MTR_4g062520 37.383 107 66 1 33 139 177 282 1.83e-12 63.9
MsG0780040611.01.T01 MTR_4g062470 37.383 107 66 1 33 139 178 283 2.36e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040611.01.T01 AT5G42500 52.941 153 69 2 3 155 36 185 8.03e-54 168
MsG0780040611.01.T01 AT1G65870 51.634 153 73 1 3 155 38 189 3.21e-51 162
MsG0780040611.01.T01 AT5G42510 50.980 153 72 2 3 155 33 182 3.37e-51 162
MsG0780040611.01.T01 AT1G22900 51.634 153 71 2 3 155 44 193 1.11e-49 158
MsG0780040611.01.T01 AT1G58170 45.806 155 83 1 2 156 32 185 3.97e-48 154
MsG0780040611.01.T01 AT5G49040 45.395 152 82 1 5 156 41 191 3.93e-47 152
MsG0780040611.01.T01 AT1G55210 45.860 157 80 3 1 156 35 187 5.51e-47 151
MsG0780040611.01.T01 AT1G55210 45.860 157 80 3 1 156 35 187 5.51e-47 151
MsG0780040611.01.T01 AT3G13650 44.805 154 81 2 3 156 37 186 7.30e-46 148
MsG0780040611.01.T01 AT2G21100 48.667 150 76 1 6 155 39 187 1.78e-43 142
MsG0780040611.01.T01 AT2G21100 48.667 150 76 1 6 155 39 187 1.78e-43 142
MsG0780040611.01.T01 AT3G13662 47.857 140 69 2 4 143 37 172 6.43e-43 140
MsG0780040611.01.T01 AT2G21110 44.371 151 79 2 10 156 37 186 3.22e-42 139
MsG0780040611.01.T01 AT4G38700 44.304 158 83 3 4 156 33 190 5.53e-40 133
MsG0780040611.01.T01 AT5G49030 46.923 130 68 1 5 134 41 169 1.60e-37 136
MsG0780040611.01.T01 AT3G58090 45.522 134 72 1 22 155 139 271 1.96e-34 121
MsG0780040611.01.T01 AT3G13660 43.697 119 66 1 38 156 8 125 3.23e-33 114
MsG0780040611.01.T01 AT4G23690 34.586 133 75 5 5 131 36 162 2.23e-13 64.7
MsG0780040611.01.T01 AT3G24020 35.789 95 59 2 40 134 126 218 7.40e-11 58.9

Find 48 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATCATGAGTGAACCTATT+AGG 0.222910 7:-79975838 MsG0780040611.01.T01:CDS
ACGATGGCCATTTCCCTAAT+AGG 0.275509 7:+79975824 None:intergenic
TGGCAGCACACTTAGTATAT+TGG 0.296246 7:-79975870 MsG0780040611.01.T01:CDS
AGGCTATACCTAACTAATAA+TGG 0.313486 7:+79975693 None:intergenic
GATCATGAGTGAACCTATTA+GGG 0.369433 7:-79975837 MsG0780040611.01.T01:CDS
GGCAGCACACTTAGTATATT+GGG 0.369856 7:-79975869 MsG0780040611.01.T01:CDS
ACAAATACCATGCTAGGTTT+TGG 0.370876 7:+79976088 None:intergenic
CACAAGTTTCGAGTCACGTT+CGG 0.378497 7:+79975985 None:intergenic
ATGGCCATCGTTGGTGGGAC+TGG 0.382946 7:-79975812 MsG0780040611.01.T01:CDS
TATTCGGTTGATTATACAAA+AGG 0.426193 7:-79975749 MsG0780040611.01.T01:CDS
TACTTTCACGACATTGTGTC+AGG 0.427188 7:-79976112 MsG0780040611.01.T01:CDS
CGTTCGGGCCCAGCCGTCAA+TGG 0.437540 7:+79976001 None:intergenic
ATGGACGATCCATTGACGGC+TGG 0.438919 7:-79976010 MsG0780040611.01.T01:CDS
GAGTGAACCTATTAGGGAAA+TGG 0.447065 7:-79975831 MsG0780040611.01.T01:CDS
TTCTATATCACAAGAGGAGA+TGG 0.477985 7:-79975939 MsG0780040611.01.T01:CDS
AGGGAAATGGCCATCGTTGG+TGG 0.488017 7:-79975818 MsG0780040611.01.T01:CDS
GAACGTGACTCGAAACTTGT+GGG 0.491012 7:-79975983 MsG0780040611.01.T01:CDS
TTCACTGATGGAGATTTCAA+TGG 0.501509 7:-79975890 MsG0780040611.01.T01:CDS
TGGACGATCCATTGACGGCT+GGG 0.502437 7:-79976009 MsG0780040611.01.T01:CDS
ACAAGTTTCGAGTCACGTTC+GGG 0.503136 7:+79975986 None:intergenic
AGCCTATCCCCAACAATGCT+GGG 0.510899 7:-79976169 None:intergenic
ATTCGGGACAGTTGTGGCGA+TGG 0.519713 7:-79976029 MsG0780040611.01.T01:CDS
AGAGGAGATGGGACTAATGA+TGG 0.528699 7:-79975927 MsG0780040611.01.T01:CDS
TGACTCGAAACTTGTGGGTA+AGG 0.534298 7:-79975978 MsG0780040611.01.T01:CDS
CTTGCCATTCGGGACAGTTG+TGG 0.537319 7:-79976035 MsG0780040611.01.T01:CDS
GGCGATGGACGATCCATTGA+CGG 0.538554 7:-79976014 MsG0780040611.01.T01:CDS
AAACTTGTGGGTAAGGCTCA+AGG 0.545821 7:-79975971 MsG0780040611.01.T01:CDS
TCAGCTACAAATACCATGCT+AGG 0.557255 7:+79976082 None:intergenic
TTACACTTCTATATCACAAG+AGG 0.558364 7:-79975945 MsG0780040611.01.T01:CDS
ATGACCATGGCATTCACTGA+TGG 0.562304 7:-79975902 MsG0780040611.01.T01:CDS
TCTATATCACAAGAGGAGAT+GGG 0.592301 7:-79975938 MsG0780040611.01.T01:CDS
CGAACGTGACTCGAAACTTG+TGG 0.592756 7:-79975984 MsG0780040611.01.T01:CDS
CGTGAAAGTAAAAGTGAAGG+TGG 0.597471 7:+79976125 None:intergenic
ATTAGGGAAATGGCCATCGT+TGG 0.598209 7:-79975821 MsG0780040611.01.T01:CDS
GGGAAATGGCCATCGTTGGT+GGG 0.599477 7:-79975817 MsG0780040611.01.T01:CDS
CACAACTGTCCCGAATGGCA+AGG 0.608603 7:+79976036 None:intergenic
ATCGCCACAACTGTCCCGAA+TGG 0.611420 7:+79976031 None:intergenic
GTCAGGACCAAAACCTAGCA+TGG 0.622028 7:-79976095 MsG0780040611.01.T01:CDS
TGTAGCTGAGCCCAATGGCA+AGG 0.624333 7:-79976068 MsG0780040611.01.T01:CDS
TACAAAAGGAGATGCCATTG+TGG 0.627394 7:-79975735 MsG0780040611.01.T01:CDS
AGCTCCAGTCCCACCAACGA+TGG 0.631050 7:+79975808 None:intergenic
ATCTCCATCAGTGAATGCCA+TGG 0.633818 7:+79975898 None:intergenic
TGTCGTGAAAGTAAAAGTGA+AGG 0.637192 7:+79976122 None:intergenic
GTATTTGTAGCTGAGCCCAA+TGG 0.650930 7:-79976073 MsG0780040611.01.T01:CDS
GCACACTTAGTATATTGGGA+AGG 0.678770 7:-79975865 MsG0780040611.01.T01:CDS
GTGAAAGTAAAAGTGAAGGT+GGG 0.693286 7:+79976126 None:intergenic
AAATATGTCATACTCCACAA+TGG 0.693639 7:+79975721 None:intergenic
ACTAATGATGGTGATGACCA+TGG 0.702330 7:-79975915 MsG0780040611.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATTCGGTTGATTATACAAA+AGG - Chr7:79976111-79976130 MsG0780040611.01.T01:CDS 25.0%
AAATATGTCATACTCCACAA+TGG + Chr7:79976142-79976161 None:intergenic 30.0%
TTACACTTCTATATCACAAG+AGG - Chr7:79975915-79975934 MsG0780040611.01.T01:CDS 30.0%
!!! TGCTCAAGCTAAGTTTTATT+CGG - Chr7:79976095-79976114 MsG0780040611.01.T01:CDS 30.0%
GATCATGAGTGAACCTATTA+GGG - Chr7:79976023-79976042 MsG0780040611.01.T01:CDS 35.0%
GTAAAAAATGCCTTGCCATT+CGG - Chr7:79975814-79975833 MsG0780040611.01.T01:CDS 35.0%
GTGAAAGTAAAAGTGAAGGT+GGG + Chr7:79975737-79975756 None:intergenic 35.0%
TAAAAAATGCCTTGCCATTC+GGG - Chr7:79975815-79975834 MsG0780040611.01.T01:CDS 35.0%
TCTATATCACAAGAGGAGAT+GGG - Chr7:79975922-79975941 MsG0780040611.01.T01:CDS 35.0%
TGATCATGAGTGAACCTATT+AGG - Chr7:79976022-79976041 MsG0780040611.01.T01:CDS 35.0%
TGTCGTGAAAGTAAAAGTGA+AGG + Chr7:79975741-79975760 None:intergenic 35.0%
TTCACTGATGGAGATTTCAA+TGG - Chr7:79975970-79975989 MsG0780040611.01.T01:CDS 35.0%
TTCTATATCACAAGAGGAGA+TGG - Chr7:79975921-79975940 MsG0780040611.01.T01:CDS 35.0%
! ACAAATACCATGCTAGGTTT+TGG + Chr7:79975775-79975794 None:intergenic 35.0%
!!! GCATTTTTTACCTTGCCATT+GGG + Chr7:79975805-79975824 None:intergenic 35.0%
CGTGAAAGTAAAAGTGAAGG+TGG + Chr7:79975738-79975757 None:intergenic 40.0%
GAGTGAACCTATTAGGGAAA+TGG - Chr7:79976029-79976048 MsG0780040611.01.T01:CDS 40.0%
GCACACTTAGTATATTGGGA+AGG - Chr7:79975995-79976014 MsG0780040611.01.T01:CDS 40.0%
GGCAGCACACTTAGTATATT+GGG - Chr7:79975991-79976010 MsG0780040611.01.T01:CDS 40.0%
TACAAAAGGAGATGCCATTG+TGG - Chr7:79976125-79976144 MsG0780040611.01.T01:CDS 40.0%
TACTTTCACGACATTGTGTC+AGG - Chr7:79975748-79975767 MsG0780040611.01.T01:CDS 40.0%
TCAGCTACAAATACCATGCT+AGG + Chr7:79975781-79975800 None:intergenic 40.0%
TGGCAGCACACTTAGTATAT+TGG - Chr7:79975990-79976009 MsG0780040611.01.T01:CDS 40.0%
! ACTAATGATGGTGATGACCA+TGG - Chr7:79975945-79975964 MsG0780040611.01.T01:CDS 40.0%
!!! GGCATTTTTTACCTTGCCAT+TGG + Chr7:79975806-79975825 None:intergenic 40.0%
AAACTTGTGGGTAAGGCTCA+AGG - Chr7:79975889-79975908 MsG0780040611.01.T01:CDS 45.0%
ACGATGGCCATTTCCCTAAT+AGG + Chr7:79976039-79976058 None:intergenic 45.0%
AGAGGAGATGGGACTAATGA+TGG - Chr7:79975933-79975952 MsG0780040611.01.T01:CDS 45.0%
ATCTCCATCAGTGAATGCCA+TGG + Chr7:79975965-79975984 None:intergenic 45.0%
ATGACCATGGCATTCACTGA+TGG - Chr7:79975958-79975977 MsG0780040611.01.T01:CDS 45.0%
ATTAGGGAAATGGCCATCGT+TGG - Chr7:79976039-79976058 MsG0780040611.01.T01:CDS 45.0%
GAACGTGACTCGAAACTTGT+GGG - Chr7:79975877-79975896 MsG0780040611.01.T01:CDS 45.0%
GTATTTGTAGCTGAGCCCAA+TGG - Chr7:79975787-79975806 MsG0780040611.01.T01:CDS 45.0%
TGACTCGAAACTTGTGGGTA+AGG - Chr7:79975882-79975901 MsG0780040611.01.T01:CDS 45.0%
! ACAAGTTTCGAGTCACGTTC+GGG + Chr7:79975877-79975896 None:intergenic 45.0%
! CACAAGTTTCGAGTCACGTT+CGG + Chr7:79975878-79975897 None:intergenic 45.0%
CGAACGTGACTCGAAACTTG+TGG - Chr7:79975876-79975895 MsG0780040611.01.T01:CDS 50.0%
GTCAGGACCAAAACCTAGCA+TGG - Chr7:79975765-79975784 MsG0780040611.01.T01:CDS 50.0%
! GGAGCTTTTCGTTTCGTACG+TGG - Chr7:79976069-79976088 MsG0780040611.01.T01:CDS 50.0%
AGGGAAATGGCCATCGTTGG+TGG - Chr7:79976042-79976061 MsG0780040611.01.T01:CDS 55.0%
ATCGCCACAACTGTCCCGAA+TGG + Chr7:79975832-79975851 None:intergenic 55.0%
ATGGACGATCCATTGACGGC+TGG - Chr7:79975850-79975869 MsG0780040611.01.T01:CDS 55.0%
ATTCGGGACAGTTGTGGCGA+TGG - Chr7:79975831-79975850 MsG0780040611.01.T01:CDS 55.0%
CACAACTGTCCCGAATGGCA+AGG + Chr7:79975827-79975846 None:intergenic 55.0%
CTTGCCATTCGGGACAGTTG+TGG - Chr7:79975825-79975844 MsG0780040611.01.T01:CDS 55.0%
GGCGATGGACGATCCATTGA+CGG - Chr7:79975846-79975865 MsG0780040611.01.T01:CDS 55.0%
GGGAAATGGCCATCGTTGGT+GGG - Chr7:79976043-79976062 MsG0780040611.01.T01:CDS 55.0%
TGGACGATCCATTGACGGCT+GGG - Chr7:79975851-79975870 MsG0780040611.01.T01:CDS 55.0%
TGTAGCTGAGCCCAATGGCA+AGG - Chr7:79975792-79975811 MsG0780040611.01.T01:CDS 55.0%
AGCTCCAGTCCCACCAACGA+TGG + Chr7:79976055-79976074 None:intergenic 60.0%
! ATGGCCATCGTTGGTGGGAC+TGG - Chr7:79976048-79976067 MsG0780040611.01.T01:CDS 60.0%
CGTTCGGGCCCAGCCGTCAA+TGG + Chr7:79975862-79975881 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 79975706 79976176 79975706 ID=MsG0780040611.01;Name=MsG0780040611.01
Chr7 mRNA 79975706 79976176 79975706 ID=MsG0780040611.01.T01;Parent=MsG0780040611.01;Name=MsG0780040611.01.T01;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|156
Chr7 exon 79975706 79976176 79975706 ID=MsG0780040611.01.T01:exon:6682;Parent=MsG0780040611.01.T01
Chr7 CDS 79975706 79976176 79975706 ID=MsG0780040611.01.T01:cds;Parent=MsG0780040611.01.T01
Gene Sequence

>MsG0780040611.01.T01

ATGCTGGGTTTTCAAGAAGAGAAATTCACCCACCTTCACTTTTACTTTCACGACATTGTGTCAGGACCAAAACCTAGCATGGTATTTGTAGCTGAGCCCAATGGCAAGGTAAAAAATGCCTTGCCATTCGGGACAGTTGTGGCGATGGACGATCCATTGACGGCTGGGCCCGAACGTGACTCGAAACTTGTGGGTAAGGCTCAAGGAATTTACACTTCTATATCACAAGAGGAGATGGGACTAATGATGGTGATGACCATGGCATTCACTGATGGAGATTTCAATGGCAGCACACTTAGTATATTGGGAAGGAACATGATCATGAGTGAACCTATTAGGGAAATGGCCATCGTTGGTGGGACTGGAGCTTTTCGTTTCGTACGTGGTTATGCTCAAGCTAAGTTTTATTCGGTTGATTATACAAAAGGAGATGCCATTGTGGAGTATGACATATTTGTGTTCCATTATTAG

Protein sequence

>MsG0780040611.01.T01

MLGFQEEKFTHLHFYFHDIVSGPKPSMVFVAEPNGKVKNALPFGTVVAMDDPLTAGPERDSKLVGKAQGIYTSISQEEMGLMMVMTMAFTDGDFNGSTLSILGRNMIMSEPIREMAIVGGTGAFRFVRGYAQAKFYSVDYTKGDAIVEYDIFVFHY*