AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018512.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018512.01.T01 MTR_4g013325 92.462 199 13 1 1 197 1 199 2.76e-117 331
MsG0480018512.01.T01 MTR_4g013350 78.788 198 40 2 1 197 1 197 4.79e-110 313
MsG0480018512.01.T01 MTR_4g013320 78.392 199 41 2 1 197 1 199 6.66e-108 308
MsG0480018512.01.T01 MTR_4g013335 80.328 183 32 3 16 197 17 196 6.84e-107 305
MsG0480018512.01.T01 MTR_4g013345 77.778 198 41 2 1 197 1 196 2.34e-106 303
MsG0480018512.01.T01 MTR_4g013330 74.000 200 48 3 1 197 1 199 2.44e-103 296
MsG0480018512.01.T01 MTR_4g013310 70.854 199 54 3 1 197 1 197 4.78e-95 275
MsG0480018512.01.T01 MTR_7g070390 68.254 189 57 2 11 197 5 192 5.34e-84 247
MsG0480018512.01.T01 MTR_4g013385 57.672 189 74 3 9 197 8 190 5.96e-76 226
MsG0480018512.01.T01 MTR_0433s0040 57.672 189 74 3 9 197 8 190 5.96e-76 226
MsG0480018512.01.T01 MTR_4g013770 60.947 169 64 2 30 197 24 191 5.07e-69 209
MsG0480018512.01.T01 MTR_1g054525 57.576 165 67 2 34 197 30 192 2.21e-64 197
MsG0480018512.01.T01 MTR_4g013355 52.381 189 75 3 9 197 6 179 1.36e-62 192
MsG0480018512.01.T01 MTR_4g073950 53.947 152 69 1 47 197 41 192 3.46e-57 179
MsG0480018512.01.T01 MTR_7g093870 51.829 164 74 2 39 197 30 193 6.70e-57 179
MsG0480018512.01.T01 MTR_7g093820 52.564 156 73 1 43 197 39 194 1.31e-55 175
MsG0480018512.01.T01 MTR_7g093850 45.078 193 97 3 7 197 9 194 1.74e-55 174
MsG0480018512.01.T01 MTR_7g093790 47.561 164 85 1 35 197 28 191 5.04e-55 173
MsG0480018512.01.T01 MTR_7g093830 48.171 164 84 1 35 197 30 193 2.36e-54 172
MsG0480018512.01.T01 MTR_1g056370 47.134 157 78 3 44 196 30 185 2.80e-47 153
MsG0480018512.01.T01 MTR_3g105640 45.963 161 84 3 40 197 28 188 8.35e-45 147
MsG0480018512.01.T01 MTR_4g078885 44.737 152 82 1 46 197 36 185 1.30e-43 144
MsG0480018512.01.T01 MTR_3g034030 45.395 152 81 1 46 197 36 185 2.16e-43 143
MsG0480018512.01.T01 MTR_3g105630 42.484 153 85 2 48 197 37 189 8.95e-41 137
MsG0480018512.01.T01 MTR_5g096120 40.196 204 96 5 4 197 2 189 1.85e-40 136
MsG0480018512.01.T01 MTR_4g122110 36.620 142 90 0 56 197 39 180 5.63e-27 101
MsG0480018512.01.T01 MTR_4g122130 33.514 185 113 2 17 197 2 180 5.72e-25 96.3
MsG0480018512.01.T01 MTR_4g013340 48.958 96 14 1 102 197 78 138 7.40e-24 92.0
MsG0480018512.01.T01 MTR_4g049550 28.966 145 103 0 48 192 29 173 6.25e-18 77.8
MsG0480018512.01.T01 MTR_4g049570 28.671 143 102 0 50 192 31 173 1.53e-17 76.6
MsG0480018512.01.T01 MTR_8g099115 34.286 140 83 3 49 180 34 172 1.79e-15 71.6
MsG0480018512.01.T01 MTR_8g099135 33.083 133 78 4 57 180 38 168 3.18e-13 65.5
MsG0480018512.01.T01 MTR_1g046800 40.000 90 53 1 84 173 135 223 2.63e-12 63.9
MsG0480018512.01.T01 MTR_1g046500 40.000 90 53 1 84 173 135 223 3.48e-12 63.5
MsG0480018512.01.T01 MTR_1g046490 36.667 90 56 1 84 173 121 209 4.61e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018512.01.T01 AT1G58170 60.215 186 67 1 12 197 7 185 8.60e-81 238
MsG0480018512.01.T01 AT5G49040 55.851 188 82 1 11 197 4 191 4.82e-75 224
MsG0480018512.01.T01 AT1G55210 55.263 190 78 3 9 197 4 187 1.82e-73 220
MsG0480018512.01.T01 AT1G55210 55.263 190 78 3 9 197 4 187 1.82e-73 220
MsG0480018512.01.T01 AT3G13650 53.763 186 83 1 12 197 4 186 3.84e-69 209
MsG0480018512.01.T01 AT5G49030 55.172 174 77 1 11 183 4 177 1.85e-58 198
MsG0480018512.01.T01 AT1G65870 55.333 150 67 0 47 196 40 189 9.95e-58 180
MsG0480018512.01.T01 AT2G21100 49.677 155 78 0 42 196 33 187 2.01e-54 172
MsG0480018512.01.T01 AT2G21100 49.677 155 78 0 42 196 33 187 2.01e-54 172
MsG0480018512.01.T01 AT3G13662 49.714 175 81 4 11 184 4 172 2.44e-53 168
MsG0480018512.01.T01 AT3G13660 65.254 118 41 0 80 197 8 125 6.20e-53 166
MsG0480018512.01.T01 AT5G42500 48.667 150 75 1 47 196 38 185 2.61e-48 156
MsG0480018512.01.T01 AT5G42510 48.667 150 75 1 47 196 35 182 1.84e-47 154
MsG0480018512.01.T01 AT1G22900 48.667 150 75 1 47 196 46 193 2.37e-47 154
MsG0480018512.01.T01 AT4G38700 43.210 162 86 3 42 197 29 190 2.18e-43 144
MsG0480018512.01.T01 AT3G58090 50.000 130 56 1 76 196 142 271 9.20e-40 137
MsG0480018512.01.T01 AT2G21110 38.462 169 95 2 33 197 23 186 8.13e-39 132
MsG0480018512.01.T01 AT4G23690 33.766 154 89 4 30 179 25 169 1.69e-16 74.3
MsG0480018512.01.T01 AT1G64160 32.639 144 90 3 41 180 25 165 7.35e-16 72.4
MsG0480018512.01.T01 AT4G11190 32.353 136 84 5 41 172 27 158 9.90e-14 67.0
MsG0480018512.01.T01 AT4G11210 29.282 181 102 7 7 180 5 166 1.76e-13 66.2

Find 35 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAAGTACACTTACTATTT+TGG 0.090168 4:-6008206 MsG0480018512.01.T01:CDS
AGTTCTGGTCCTAAAGTTAA+AGG 0.191161 4:+6008337 None:intergenic
ATTGACAACCCTTTAACTTT+AGG 0.265955 4:-6008346 MsG0480018512.01.T01:CDS
CTAAACACAACCACTGCTTT+TGG 0.320348 4:-6008382 MsG0480018512.01.T01:CDS
TTTATTGAAGGAAAGTATAA+TGG 0.324124 4:-6008226 MsG0480018512.01.T01:CDS
GTGGTTGTGTTTAGTTTCAT+TGG 0.341628 4:+6008391 None:intergenic
AGATGAGATTGATTTGCTTA+TGG 0.352366 4:-6008263 MsG0480018512.01.T01:CDS
CTTAATAATTTGCGGTCTAT+AGG 0.353141 4:+6008505 None:intergenic
GAACTGAGTTCTAAACTTGT+TGG 0.378339 4:-6008319 MsG0480018512.01.T01:CDS
CATTGGTTTGATATCAAAAC+CGG 0.379656 4:-6008088 MsG0480018512.01.T01:CDS
AAGTGATGGTGTTGCAAGAT+AGG 0.403929 4:+6008575 None:intergenic
TCTTTAGGCTTAATAATTTG+CGG 0.414614 4:+6008497 None:intergenic
GTTCTGGTCCTAAAGTTAAA+GGG 0.426776 4:+6008338 None:intergenic
ACAAGTTTAGAACTCAGTTC+TGG 0.427865 4:+6008322 None:intergenic
ATGCTGAAGCTAATACTCAT+TGG 0.445682 4:-6008105 MsG0480018512.01.T01:CDS
GTGTTTAGTTTCATTGGTGG+TGG 0.448998 4:+6008397 None:intergenic
GGAACAACTGCAACTGAAGT+AGG 0.473877 4:+6008418 None:intergenic
AGAACAAAGTGATGAAGTGT+TGG 0.478622 4:+6008608 None:intergenic
GAACAAAGTGATGAAGTGTT+GGG 0.498072 4:+6008609 None:intergenic
TAAGTCATTTCAGATTCTAC+TGG 0.503837 4:-6008465 MsG0480018512.01.T01:CDS
CAAATTATTAAGCCTAAAGA+AGG 0.508677 4:-6008494 MsG0480018512.01.T01:CDS
AAACTTGTTGGAAAAGCTCA+AGG 0.509980 4:-6008307 MsG0480018512.01.T01:CDS
TGGTGTTGCAAGATAGGAGA+AGG 0.539243 4:+6008581 None:intergenic
ATGCCTGTGATTGGTGGCAG+TGG 0.540114 4:-6008151 MsG0480018512.01.T01:CDS
GTTGTGTTTAGTTTCATTGG+TGG 0.544359 4:+6008394 None:intergenic
ATACTCAACAATAGCATCCC+CGG 0.565003 4:+6008069 None:intergenic
GTTAGAGAGATGCCTGTGAT+TGG 0.567283 4:-6008160 MsG0480018512.01.T01:CDS
GAACAACTGCAACTGAAGTA+GGG 0.596771 4:+6008419 None:intergenic
AATCCACTGCCACCAATCAC+AGG 0.619144 4:+6008148 None:intergenic
AGAGAGATGCCTGTGATTGG+TGG 0.622674 4:-6008157 MsG0480018512.01.T01:CDS
TGATGATGATGTTAAAGTGA+TGG 0.638584 4:+6008561 None:intergenic
TACTGGCACGACATAGTGAG+TGG 0.639000 4:-6008448 MsG0480018512.01.T01:CDS
ATTGGTTTGATATCAAAACC+GGG 0.644981 4:-6008087 MsG0480018512.01.T01:CDS
GTTGAGTAAACCAAAAGCAG+TGG 0.663208 4:+6008372 None:intergenic
TTGGTTTGATATCAAAACCG+GGG 0.681345 4:-6008086 MsG0480018512.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGAATTTTGCTTTTATTGA+AGG - Chr4:6008423-6008442 MsG0480018512.01.T01:CDS 20.0%
!!! TTTATTGAAGGAAAGTATAA+TGG - Chr4:6008435-6008454 MsG0480018512.01.T01:CDS 20.0%
! CAAATTATTAAGCCTAAAGA+AGG - Chr4:6008167-6008186 MsG0480018512.01.T01:CDS 25.0%
! TCTTTAGGCTTAATAATTTG+CGG + Chr4:6008167-6008186 None:intergenic 25.0%
TAAGTCATTTCAGATTCTAC+TGG - Chr4:6008196-6008215 MsG0480018512.01.T01:CDS 30.0%
! ATTGACAACCCTTTAACTTT+AGG - Chr4:6008315-6008334 MsG0480018512.01.T01:CDS 30.0%
! CTTAATAATTTGCGGTCTAT+AGG + Chr4:6008159-6008178 None:intergenic 30.0%
! TGATGATGATGTTAAAGTGA+TGG + Chr4:6008103-6008122 None:intergenic 30.0%
!! AGATGAGATTGATTTGCTTA+TGG - Chr4:6008398-6008417 MsG0480018512.01.T01:CDS 30.0%
!! ATTGGTTTGATATCAAAACC+GGG - Chr4:6008574-6008593 MsG0480018512.01.T01:CDS 30.0%
!! CATTGGTTTGATATCAAAAC+CGG - Chr4:6008573-6008592 MsG0480018512.01.T01:CDS 30.0%
!! GACTTAGTTTTTCCTTCTTT+AGG + Chr4:6008182-6008201 None:intergenic 30.0%
!! GGAAGTACACTTACTATTTT+GGG - Chr4:6008456-6008475 MsG0480018512.01.T01:CDS 30.0%
!! TGGAAGTACACTTACTATTT+TGG - Chr4:6008455-6008474 MsG0480018512.01.T01:CDS 30.0%
AGAACAAAGTGATGAAGTGT+TGG + Chr4:6008056-6008075 None:intergenic 35.0%
ATGCTGAAGCTAATACTCAT+TGG - Chr4:6008556-6008575 MsG0480018512.01.T01:CDS 35.0%
GTTCTGGTCCTAAAGTTAAA+GGG + Chr4:6008326-6008345 None:intergenic 35.0%
! ACAAGTTTAGAACTCAGTTC+TGG + Chr4:6008342-6008361 None:intergenic 35.0%
! AGTTCTGGTCCTAAAGTTAA+AGG + Chr4:6008327-6008346 None:intergenic 35.0%
! GAACAAAGTGATGAAGTGTT+GGG + Chr4:6008055-6008074 None:intergenic 35.0%
! GTGGTTGTGTTTAGTTTCAT+TGG + Chr4:6008273-6008292 None:intergenic 35.0%
! GTTGTGTTTAGTTTCATTGG+TGG + Chr4:6008270-6008289 None:intergenic 35.0%
! TTGGTTTGATATCAAAACCG+GGG - Chr4:6008575-6008594 MsG0480018512.01.T01:CDS 35.0%
!! AAACTTGTTGGAAAAGCTCA+AGG - Chr4:6008354-6008373 MsG0480018512.01.T01:CDS 35.0%
!! GAACTGAGTTCTAAACTTGT+TGG - Chr4:6008342-6008361 MsG0480018512.01.T01:CDS 35.0%
!! GTACACTTACTATTTTGGGA+AGG - Chr4:6008460-6008479 MsG0480018512.01.T01:CDS 35.0%
!!! GGATTTTTCAGATTTGCTAG+AGG - Chr4:6008531-6008550 MsG0480018512.01.T01:CDS 35.0%
ATACTCAACAATAGCATCCC+CGG + Chr4:6008595-6008614 None:intergenic 40.0%
GAACAACTGCAACTGAAGTA+GGG + Chr4:6008245-6008264 None:intergenic 40.0%
GTTGAGTAAACCAAAAGCAG+TGG + Chr4:6008292-6008311 None:intergenic 40.0%
! CTAAACACAACCACTGCTTT+TGG - Chr4:6008279-6008298 MsG0480018512.01.T01:CDS 40.0%
! GTGTTTAGTTTCATTGGTGG+TGG + Chr4:6008267-6008286 None:intergenic 40.0%
!! AAGTGATGGTGTTGCAAGAT+AGG + Chr4:6008089-6008108 None:intergenic 40.0%
GGAACAACTGCAACTGAAGT+AGG + Chr4:6008246-6008265 None:intergenic 45.0%
GTTAGAGAGATGCCTGTGAT+TGG - Chr4:6008501-6008520 MsG0480018512.01.T01:CDS 45.0%
!! TGGTGTTGCAAGATAGGAGA+AGG + Chr4:6008083-6008102 None:intergenic 45.0%
AATCCACTGCCACCAATCAC+AGG + Chr4:6008516-6008535 None:intergenic 50.0%
AGAGAGATGCCTGTGATTGG+TGG - Chr4:6008504-6008523 MsG0480018512.01.T01:CDS 50.0%
TACTGGCACGACATAGTGAG+TGG - Chr4:6008213-6008232 MsG0480018512.01.T01:CDS 50.0%
! ATGCCTGTGATTGGTGGCAG+TGG - Chr4:6008510-6008529 MsG0480018512.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 6008045 6008638 6008045 ID=MsG0480018512.01;Name=MsG0480018512.01
Chr4 mRNA 6008045 6008638 6008045 ID=MsG0480018512.01.T01;Parent=MsG0480018512.01;Name=MsG0480018512.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|197
Chr4 exon 6008045 6008638 6008045 ID=MsG0480018512.01.T01:exon:2133;Parent=MsG0480018512.01.T01
Chr4 CDS 6008045 6008638 6008045 ID=MsG0480018512.01.T01:cds;Parent=MsG0480018512.01.T01
Gene Sequence

>MsG0480018512.01.T01

ATGCAAACCCAACACTTCATCACTTTGTTCTTCTTCCTTCTCCTATCTTGCAACACCATCACTTTAACATCATCATCATCAGAAAATGATGACACTTTTGAATTTGTTCGTCCTATAGACCGCAAATTATTAAGCCTAAAGAAGGAAAAACTAAGTCATTTCAGATTCTACTGGCACGACATAGTGAGTGGAAAAAACCCTACTTCAGTTGCAGTTGTTCCACCACCAATGAAACTAAACACAACCACTGCTTTTGGTTTACTCAACATGATTGACAACCCTTTAACTTTAGGACCAGAACTGAGTTCTAAACTTGTTGGAAAAGCTCAAGGTTTCTATGCTTCTGCATGTAAAGATGAGATTGATTTGCTTATGGCAATGAATTTTGCTTTTATTGAAGGAAAGTATAATGGAAGTACACTTACTATTTTGGGAAGGAATGCTGTTTTTCATAAAGTTAGAGAGATGCCTGTGATTGGTGGCAGTGGATTTTTCAGATTTGCTAGAGGATATGCTGAAGCTAATACTCATTGGTTTGATATCAAAACCGGGGATGCTATTGTTGAGTATAATGTTTATGTTTTTCACTATTGA

Protein sequence

>MsG0480018512.01.T01

MQTQHFITLFFFLLLSCNTITLTSSSSENDDTFEFVRPIDRKLLSLKKEKLSHFRFYWHDIVSGKNPTSVAVVPPPMKLNTTTAFGLLNMIDNPLTLGPELSSKLVGKAQGFYASACKDEIDLLMAMNFAFIEGKYNGSTLTILGRNAVFHKVREMPVIGGSGFFRFARGYAEANTHWFDIKTGDAIVEYNVYVFHY*