Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040867.01.T01 | KEH24003.1 | 97.917 | 240 | 5 | 0 | 1 | 240 | 98 | 337 | 9.27E-170 | 484 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040867.01.T01 | Q9LS48 | 36.975 | 238 | 131 | 5 | 2 | 231 | 74 | 300 | 2.29E-42 | 150 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040867.01.T01 | A0A072U4F2 | 97.917 | 240 | 5 | 0 | 1 | 240 | 98 | 337 | 4.43e-170 | 484 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780040867.01 | MsG0880043905.01 | 0.800676 | 1.288575e-48 | 6.036413e-46 |
| MsG0780040867.01 | MsG0880044976.01 | 0.813104 | 3.030243e-51 | 1.960815e-48 |
| MsG0780040867.01 | MsG0880046699.01 | 0.822738 | 2.017579e-53 | 1.703283e-50 |
| MsG0780040867.01 | MsG0880046827.01 | 0.850361 | 1.784309e-60 | 3.486278e-57 |
| MsG0280011261.01 | MsG0780040867.01 | 0.828847 | 7.161450e-55 | 7.202488e-52 |
| MsG0280011424.01 | MsG0780040867.01 | 0.803285 | 3.749077e-49 | 1.876581e-46 |
| MsG0380014277.01 | MsG0780040867.01 | 0.814057 | 1.869873e-51 | 1.241772e-48 |
| MsG0380014287.01 | MsG0780040867.01 | 0.833329 | 5.678983e-56 | 6.519199e-53 |
| MsG0680032378.01 | MsG0780040867.01 | 0.813857 | 2.069594e-51 | 1.366951e-48 |
| MsG0480021764.01 | MsG0780040867.01 | 0.808959 | 2.396406e-50 | 1.389070e-47 |
| MsG0580025261.01 | MsG0780040867.01 | 0.835438 | 1.677903e-56 | 2.051812e-53 |
| MsG0380015304.01 | MsG0780040867.01 | 0.827929 | 1.192256e-54 | 1.167579e-51 |
| MsG0380015703.01 | MsG0780040867.01 | 0.832794 | 7.711359e-56 | 8.711044e-53 |
| MsG0480018426.01 | MsG0780040867.01 | 0.811914 | 5.514889e-51 | 3.456902e-48 |
| MsG0580026202.01 | MsG0780040867.01 | 0.806943 | 6.433588e-50 | 3.537612e-47 |
| MsG0580026921.01 | MsG0780040867.01 | 0.814395 | 1.574647e-51 | 1.055409e-48 |
| MsG0580029355.01 | MsG0780040867.01 | 0.801052 | 1.079872e-48 | 5.107454e-46 |
| MsG0280007417.01 | MsG0780040867.01 | 0.812877 | 3.397618e-51 | 2.185323e-48 |
| MsG0780036705.01 | MsG0780040867.01 | 0.805451 | 1.326330e-49 | 7.019439e-47 |
| MsG0780039429.01 | MsG0780040867.01 | 0.835374 | 1.741805e-56 | 2.125951e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0380016764.01 | MsG0780040867.01 | PPI |
| MsG0780040867.01 | MsG0480022848.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040867.01.T01 | MTR_7g100328 | 97.917 | 240 | 5 | 0 | 1 | 240 | 98 | 337 | 1.12e-173 | 484 |
| MsG0780040867.01.T01 | MTR_1g067600 | 48.178 | 247 | 110 | 4 | 1 | 234 | 96 | 337 | 1.39e-71 | 224 |
| MsG0780040867.01.T01 | MTR_3g095070 | 42.029 | 207 | 114 | 2 | 28 | 233 | 107 | 308 | 1.60e-46 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040867.01.T01 | AT3G53560 | 62.996 | 227 | 78 | 3 | 21 | 246 | 108 | 329 | 1.76e-93 | 280 |
| MsG0780040867.01.T01 | AT3G53560 | 62.996 | 227 | 78 | 3 | 21 | 246 | 156 | 377 | 5.43e-93 | 280 |
| MsG0780040867.01.T01 | AT2G37400 | 63.981 | 211 | 71 | 2 | 37 | 246 | 123 | 329 | 7.32e-91 | 273 |
| MsG0780040867.01.T01 | AT5G02590 | 59.406 | 202 | 77 | 2 | 37 | 238 | 122 | 318 | 1.15e-82 | 252 |
| MsG0780040867.01.T01 | AT3G09490 | 43.062 | 209 | 115 | 1 | 29 | 237 | 98 | 302 | 1.67e-52 | 175 |
| MsG0780040867.01.T01 | AT4G39470 | 36.986 | 219 | 130 | 2 | 15 | 233 | 106 | 316 | 1.80e-48 | 164 |
| MsG0780040867.01.T01 | AT3G18420 | 36.975 | 238 | 131 | 5 | 2 | 231 | 74 | 300 | 2.33e-43 | 150 |
Find 47 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTATTGGTTGCTCAGATTA+AGG | 0.260166 | 7:+83653852 | MsG0780040867.01.T01:CDS |
| AGAAGAAGGTTTCGGATGTT+AGG | 0.266026 | 7:+83653821 | MsG0780040867.01.T01:CDS |
| CTTCGAGTTGAAGCATTTCA+TGG | 0.282568 | 7:+83653700 | MsG0780040867.01.T01:CDS |
| TCATCAAAATACTCTTTATA+AGG | 0.300941 | 7:-83654048 | None:intergenic |
| AGAGGCGCTTAAAGCTTATC+AGG | 0.305548 | 7:+83653894 | MsG0780040867.01.T01:CDS |
| ACAATTTCTTAGCAGATTCA+TGG | 0.328436 | 7:-83653651 | None:intergenic |
| CGTAGAGCTTCAGCATCATT+TGG | 0.335421 | 7:-83653493 | None:intergenic |
| TCTTTATAAGGATGATTCTT+CGG | 0.343610 | 7:-83654036 | None:intergenic |
| GCTTAAAGCTTATCAGGATT+TGG | 0.343744 | 7:+83653900 | MsG0780040867.01.T01:CDS |
| TGAAGCTCTACGTGCTTTAA+TGG | 0.348747 | 7:+83653504 | MsG0780040867.01.T01:CDS |
| CTTTATAAGGATGATTCTTC+GGG | 0.354190 | 7:-83654035 | None:intergenic |
| TGGACTTGTGATGGCGAGTT+CGG | 0.402706 | 7:+83653720 | MsG0780040867.01.T01:CDS |
| CAGGTTCAATTTCAATCAAA+CGG | 0.412642 | 7:-83653576 | None:intergenic |
| AATTGCCACTCCATTTCTTC+AGG | 0.420552 | 7:-83653595 | None:intergenic |
| TGAAATTGAACCTGAAGAAA+TGG | 0.424458 | 7:+83653585 | MsG0780040867.01.T01:CDS |
| GAAGAAGGTTTCGGATGTTA+GGG | 0.426707 | 7:+83653822 | MsG0780040867.01.T01:CDS |
| CAAGAAACAGAAGAAGGTTT+CGG | 0.449162 | 7:+83653813 | MsG0780040867.01.T01:CDS |
| GAGAGAGTGGCGGCGATGAC+AGG | 0.450025 | 7:-83653391 | None:intergenic |
| ATCTGCTAAGAAATTGTTTG+AGG | 0.450418 | 7:+83653657 | MsG0780040867.01.T01:CDS |
| CTTCCCTCTCGAATTTCTGC+GGG | 0.460361 | 7:-83654094 | None:intergenic |
| TTCATCTTTCTTTCTAAGCA+AGG | 0.478456 | 7:-83653977 | None:intergenic |
| AATGGAGTGGCAATTGTTGA+AGG | 0.525932 | 7:+83653603 | MsG0780040867.01.T01:CDS |
| CTCTTTAACAATCCTTTCAA+CGG | 0.526642 | 7:-83653766 | None:intergenic |
| AGAGGGAAGAAGCTGGTGCT+AGG | 0.536510 | 7:+83654108 | MsG0780040867.01.T01:CDS |
| GGTTGCTCAGATTAAGGTGA+TGG | 0.536546 | 7:+83653858 | MsG0780040867.01.T01:CDS |
| CTCTCCTCCGACCACCGTAA+CGG | 0.540098 | 7:+83653411 | MsG0780040867.01.T01:CDS |
| TCTTCCCTCTCGAATTTCTG+CGG | 0.553717 | 7:-83654095 | None:intergenic |
| CAATCTGATGAACCGTTGAA+AGG | 0.557133 | 7:+83653754 | MsG0780040867.01.T01:CDS |
| ATAATACCCTGACACAGATA+AGG | 0.564227 | 7:-83653949 | None:intergenic |
| AACGGAAAATGCTACGACGG+AGG | 0.575798 | 7:+83653429 | MsG0780040867.01.T01:CDS |
| GCTCAGATTAAGGTGATGGA+AGG | 0.579155 | 7:+83653862 | MsG0780040867.01.T01:CDS |
| AGATAAGGCCTAAAATCTCT+CGG | 0.585108 | 7:-83653934 | None:intergenic |
| TTTAGGCCTTATCTGTGTCA+GGG | 0.591449 | 7:+83653943 | MsG0780040867.01.T01:CDS |
| CGGCGATGACAGGAGTGTGA+TGG | 0.592621 | 7:-83653381 | None:intergenic |
| AGCATTTCATGGACTTGTGA+TGG | 0.594852 | 7:+83653711 | MsG0780040867.01.T01:CDS |
| GTTGTGCAAGAAACAGAAGA+AGG | 0.595826 | 7:+83653807 | MsG0780040867.01.T01:CDS |
| AAATTCGAGAGGGAAGAAGC+TGG | 0.597290 | 7:+83654101 | MsG0780040867.01.T01:CDS |
| GCTTAGAAAGAAAGATGAAG+CGG | 0.617032 | 7:+83653981 | MsG0780040867.01.T01:CDS |
| CTCAGATTAAGGTGATGGAA+GGG | 0.621477 | 7:+83653863 | MsG0780040867.01.T01:CDS |
| TGAAATGCTTCAACTCGAAG+CGG | 0.637606 | 7:-83653697 | None:intergenic |
| TTGAACCTGAAGAAATGGAG+TGG | 0.639379 | 7:+83653590 | MsG0780040867.01.T01:CDS |
| GGTGGTCGGAGGAGAGAGAG+TGG | 0.645390 | 7:-83653404 | None:intergenic |
| AGCTCTACGTGCTTTAATGG+AGG | 0.672443 | 7:+83653507 | MsG0780040867.01.T01:CDS |
| CGTAACGGAAAATGCTACGA+CGG | 0.678661 | 7:+83653426 | MsG0780040867.01.T01:CDS |
| TTTCCCGCAGAAATTCGAGA+GGG | 0.688033 | 7:+83654091 | MsG0780040867.01.T01:CDS |
| GGTCGGAGGAGAGAGAGTGG+CGG | 0.713800 | 7:-83653401 | None:intergenic |
| GAGCAGAAGATTCTACTGAG+AGG | 0.729391 | 7:+83654141 | MsG0780040867.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCGATAAAAACTCATATA+AGG | - | Chr7:83654168-83654187 | None:intergenic | 20.0% |
| !! | TCATCAAAATACTCTTTATA+AGG | - | Chr7:83654051-83654070 | None:intergenic | 20.0% |
| !!! | AGTTTTTATCGATTTACTTT+TGG | + | Chr7:83654174-83654193 | MsG0780040867.01.T01:CDS | 20.0% |
| !!! | TATTTTGATGATAACATGAA+TGG | + | Chr7:83654060-83654079 | MsG0780040867.01.T01:CDS | 20.0% |
| !!! | TTTTTAGTTGAATGCTTTTT+CGG | + | Chr7:83654207-83654226 | MsG0780040867.01.T01:CDS | 20.0% |
| ! | TCTTTATAAGGATGATTCTT+CGG | - | Chr7:83654039-83654058 | None:intergenic | 25.0% |
| AAAGAGAGTTGAAAAAGCAA+TGG | + | Chr7:83653783-83653802 | MsG0780040867.01.T01:CDS | 30.0% | |
| ACAATTTCTTAGCAGATTCA+TGG | - | Chr7:83653654-83653673 | None:intergenic | 30.0% | |
| AGAAAAATTGATGAAGCGAT+TGG | + | Chr7:83653544-83653563 | MsG0780040867.01.T01:CDS | 30.0% | |
| ATCTGCTAAGAAATTGTTTG+AGG | + | Chr7:83653657-83653676 | MsG0780040867.01.T01:CDS | 30.0% | |
| CAGGTTCAATTTCAATCAAA+CGG | - | Chr7:83653579-83653598 | None:intergenic | 30.0% | |
| CTCTTTAACAATCCTTTCAA+CGG | - | Chr7:83653769-83653788 | None:intergenic | 30.0% | |
| CTTTATAAGGATGATTCTTC+GGG | - | Chr7:83654038-83654057 | None:intergenic | 30.0% | |
| TGAAATTGAACCTGAAGAAA+TGG | + | Chr7:83653585-83653604 | MsG0780040867.01.T01:CDS | 30.0% | |
| TTCATCTTTCTTTCTAAGCA+AGG | - | Chr7:83653980-83653999 | None:intergenic | 30.0% | |
| ! | TTCAGCATCATTTGGATTTT+CGG | - | Chr7:83653488-83653507 | None:intergenic | 30.0% |
| !!! | TGTTAGGGATTTTAGGTTAT+TGG | + | Chr7:83653837-83653856 | MsG0780040867.01.T01:CDS | 30.0% |
| AGATAAGGCCTAAAATCTCT+CGG | - | Chr7:83653937-83653956 | None:intergenic | 35.0% | |
| ATAATACCCTGACACAGATA+AGG | - | Chr7:83653952-83653971 | None:intergenic | 35.0% | |
| CAAGAAACAGAAGAAGGTTT+CGG | + | Chr7:83653813-83653832 | MsG0780040867.01.T01:CDS | 35.0% | |
| GCTTAAAGCTTATCAGGATT+TGG | + | Chr7:83653900-83653919 | MsG0780040867.01.T01:CDS | 35.0% | |
| GCTTAGAAAGAAAGATGAAG+CGG | + | Chr7:83653981-83654000 | MsG0780040867.01.T01:CDS | 35.0% | |
| ! | AAGAAGAACCGAGAGATTTT+AGG | + | Chr7:83653926-83653945 | MsG0780040867.01.T01:CDS | 35.0% |
| ! | GTTATTGGTTGCTCAGATTA+AGG | + | Chr7:83653852-83653871 | MsG0780040867.01.T01:CDS | 35.0% |
| ! | TTTCGGATGTTAGGGATTTT+AGG | + | Chr7:83653830-83653849 | MsG0780040867.01.T01:CDS | 35.0% |
| !!! | GATTTACTTTTGGTGCTCTA+TGG | + | Chr7:83654184-83654203 | MsG0780040867.01.T01:CDS | 35.0% |
| AATGGAGTGGCAATTGTTGA+AGG | + | Chr7:83653603-83653622 | MsG0780040867.01.T01:CDS | 40.0% | |
| AATTGCCACTCCATTTCTTC+AGG | - | Chr7:83653598-83653617 | None:intergenic | 40.0% | |
| AGAAGAAGGTTTCGGATGTT+AGG | + | Chr7:83653821-83653840 | MsG0780040867.01.T01:CDS | 40.0% | |
| AGCATTTCATGGACTTGTGA+TGG | + | Chr7:83653711-83653730 | MsG0780040867.01.T01:CDS | 40.0% | |
| CAATCTGATGAACCGTTGAA+AGG | + | Chr7:83653754-83653773 | MsG0780040867.01.T01:CDS | 40.0% | |
| GAAGAAGGTTTCGGATGTTA+GGG | + | Chr7:83653822-83653841 | MsG0780040867.01.T01:CDS | 40.0% | |
| GTTGTGCAAGAAACAGAAGA+AGG | + | Chr7:83653807-83653826 | MsG0780040867.01.T01:CDS | 40.0% | |
| TGAAATGCTTCAACTCGAAG+CGG | - | Chr7:83653700-83653719 | None:intergenic | 40.0% | |
| TGAAGCTCTACGTGCTTTAA+TGG | + | Chr7:83653504-83653523 | MsG0780040867.01.T01:CDS | 40.0% | |
| TTGAACCTGAAGAAATGGAG+TGG | + | Chr7:83653590-83653609 | MsG0780040867.01.T01:CDS | 40.0% | |
| ! | TTTAGGCCTTATCTGTGTCA+GGG | + | Chr7:83653943-83653962 | MsG0780040867.01.T01:CDS | 40.0% |
| !! | CTCAGATTAAGGTGATGGAA+GGG | + | Chr7:83653863-83653882 | MsG0780040867.01.T01:CDS | 40.0% |
| !! | CTTCGAGTTGAAGCATTTCA+TGG | + | Chr7:83653700-83653719 | MsG0780040867.01.T01:CDS | 40.0% |
| !! | GATGGAAGGGAATTTTTCAG+AGG | + | Chr7:83653876-83653895 | MsG0780040867.01.T01:CDS | 40.0% |
| !! | TTTTAGGCCTTATCTGTGTC+AGG | + | Chr7:83653942-83653961 | MsG0780040867.01.T01:CDS | 40.0% |
| AAATTCGAGAGGGAAGAAGC+TGG | + | Chr7:83654101-83654120 | MsG0780040867.01.T01:CDS | 45.0% | |
| AGCTCTACGTGCTTTAATGG+AGG | + | Chr7:83653507-83653526 | MsG0780040867.01.T01:CDS | 45.0% | |
| CGTAGAGCTTCAGCATCATT+TGG | - | Chr7:83653496-83653515 | None:intergenic | 45.0% | |
| GAGCAGAAGATTCTACTGAG+AGG | + | Chr7:83654141-83654160 | MsG0780040867.01.T01:CDS | 45.0% | |
| GGTTGCTCAGATTAAGGTGA+TGG | + | Chr7:83653858-83653877 | MsG0780040867.01.T01:CDS | 45.0% | |
| TCTTCCCTCTCGAATTTCTG+CGG | - | Chr7:83654098-83654117 | None:intergenic | 45.0% | |
| TTTCCCGCAGAAATTCGAGA+GGG | + | Chr7:83654091-83654110 | MsG0780040867.01.T01:CDS | 45.0% | |
| ! | CGTAACGGAAAATGCTACGA+CGG | + | Chr7:83653426-83653445 | MsG0780040867.01.T01:CDS | 45.0% |
| ! | CGTCGTAGCATTTTCCGTTA+CGG | - | Chr7:83653428-83653447 | None:intergenic | 45.0% |
| ! | TTTTCCCGCAGAAATTCGAG+AGG | + | Chr7:83654090-83654109 | MsG0780040867.01.T01:CDS | 45.0% |
| !! | AGAGGCGCTTAAAGCTTATC+AGG | + | Chr7:83653894-83653913 | MsG0780040867.01.T01:CDS | 45.0% |
| !! | GCTCAGATTAAGGTGATGGA+AGG | + | Chr7:83653862-83653881 | MsG0780040867.01.T01:CDS | 45.0% |
| AACGGAAAATGCTACGACGG+AGG | + | Chr7:83653429-83653448 | MsG0780040867.01.T01:CDS | 50.0% | |
| CTTCCCTCTCGAATTTCTGC+GGG | - | Chr7:83654097-83654116 | None:intergenic | 50.0% | |
| ! | CGTAGCATTTTCCGTTACGG+TGG | - | Chr7:83653425-83653444 | None:intergenic | 50.0% |
| ! | GCATTTTCCGTTACGGTGGT+CGG | - | Chr7:83653421-83653440 | None:intergenic | 50.0% |
| ! | TGGACTTGTGATGGCGAGTT+CGG | + | Chr7:83653720-83653739 | MsG0780040867.01.T01:CDS | 50.0% |
| AGAGGGAAGAAGCTGGTGCT+AGG | + | Chr7:83654108-83654127 | MsG0780040867.01.T01:CDS | 55.0% | |
| ! | TTTTCCGTTACGGTGGTCGG+AGG | - | Chr7:83653418-83653437 | None:intergenic | 55.0% |
| CTCTCCTCCGACCACCGTAA+CGG | + | Chr7:83653411-83653430 | MsG0780040867.01.T01:CDS | 60.0% | |
| ! | CGGCGATGACAGGAGTGTGA+TGG | - | Chr7:83653384-83653403 | None:intergenic | 60.0% |
| GAGAGAGTGGCGGCGATGAC+AGG | - | Chr7:83653394-83653413 | None:intergenic | 65.0% | |
| GGTCGGAGGAGAGAGAGTGG+CGG | - | Chr7:83653404-83653423 | None:intergenic | 65.0% | |
| GGTGGTCGGAGGAGAGAGAG+TGG | - | Chr7:83653407-83653426 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 83653373 | 83654233 | 83653373 | ID=MsG0780040867.01;Name=MsG0780040867.01 |
| Chr7 | mRNA | 83653373 | 83654233 | 83653373 | ID=MsG0780040867.01.T01;Parent=MsG0780040867.01;Name=MsG0780040867.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|286 |
| Chr7 | exon | 83653373 | 83654233 | 83653373 | ID=MsG0780040867.01.T01:exon:19872;Parent=MsG0780040867.01.T01 |
| Chr7 | CDS | 83653373 | 83654233 | 83653373 | ID=MsG0780040867.01.T01:cds;Parent=MsG0780040867.01.T01 |
| Gene Sequence |
| Protein sequence |
