Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032378.01.T01 | XP_013451991.1 | 85.23 | 738 | 36 | 5 | 1 | 719 | 1 | 684 | 0 | 1167 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048748.01 | MsG0680032378.01 | 0.845902 | 3.039950e-59 | 5.142908e-56 |
| MsG0080048849.01 | MsG0680032378.01 | 0.860877 | 1.520581e-63 | 4.219593e-60 |
| MsG0080048878.01 | MsG0680032378.01 | 0.841053 | 6.000941e-58 | 8.711594e-55 |
| MsG0080049069.01 | MsG0680032378.01 | 0.809309 | 2.016271e-50 | 1.179744e-47 |
| MsG0180000026.01 | MsG0680032378.01 | 0.810669 | 1.027334e-50 | 6.231149e-48 |
| MsG0180000113.01 | MsG0680032378.01 | 0.878506 | 2.656887e-69 | 1.391062e-65 |
| MsG0180000747.01 | MsG0680032378.01 | 0.830360 | 3.068595e-55 | 3.225840e-52 |
| MsG0180001609.01 | MsG0680032378.01 | 0.829142 | 6.073356e-55 | 6.161943e-52 |
| MsG0180001704.01 | MsG0680032378.01 | 0.815842 | 7.513568e-52 | 5.237624e-49 |
| MsG0180001722.01 | MsG0680032378.01 | 0.837407 | 5.294940e-57 | 6.870707e-54 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032378.01.T01 | MTR_6g039840 | 85.230 | 738 | 36 | 5 | 1 | 719 | 1 | 684 | 0.0 | 1167 |
| MsG0680032378.01.T01 | MTR_6g039840 | 82.734 | 556 | 29 | 2 | 183 | 719 | 1 | 508 | 0.0 | 868 |
| MsG0680032378.01.T01 | MTR_6g040070 | 39.245 | 265 | 136 | 6 | 62 | 319 | 530 | 776 | 2.29e-48 | 183 |
| MsG0680032378.01.T01 | MTR_2g100230 | 28.864 | 440 | 247 | 8 | 100 | 537 | 80 | 455 | 8.77e-48 | 176 |
| MsG0680032378.01.T01 | MTR_3g118120 | 30.853 | 457 | 237 | 14 | 116 | 550 | 132 | 531 | 2.10e-47 | 176 |
| MsG0680032378.01.T01 | MTR_3g467550 | 28.982 | 452 | 237 | 11 | 114 | 550 | 115 | 497 | 1.51e-46 | 174 |
| MsG0680032378.01.T01 | MTR_3g467550 | 29.673 | 428 | 217 | 11 | 138 | 550 | 108 | 466 | 7.57e-46 | 172 |
| MsG0680032378.01.T01 | MTR_6g082080 | 29.792 | 433 | 228 | 10 | 116 | 543 | 10 | 371 | 2.45e-45 | 168 |
| MsG0680032378.01.T01 | MTR_7g056830 | 29.561 | 433 | 229 | 10 | 116 | 543 | 10 | 371 | 6.80e-45 | 167 |
| MsG0680032378.01.T01 | MTR_3g083690 | 27.646 | 463 | 262 | 10 | 102 | 549 | 128 | 532 | 7.67e-45 | 170 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032378.01.T01 | AT4G09730 | 53.868 | 711 | 210 | 11 | 10 | 712 | 18 | 618 | 0.0 | 665 |
| MsG0680032378.01.T01 | AT3G06980 | 29.901 | 505 | 259 | 17 | 74 | 545 | 323 | 765 | 1.52e-51 | 192 |
| MsG0680032378.01.T01 | AT1G55150 | 28.097 | 452 | 261 | 7 | 93 | 543 | 77 | 465 | 4.32e-47 | 174 |
| MsG0680032378.01.T01 | AT1G55150 | 28.097 | 452 | 261 | 7 | 93 | 543 | 102 | 490 | 4.69e-46 | 172 |
| MsG0680032378.01.T01 | AT3G01540 | 29.861 | 432 | 220 | 13 | 116 | 537 | 159 | 517 | 1.61e-45 | 172 |
| MsG0680032378.01.T01 | AT3G01540 | 29.861 | 432 | 220 | 13 | 116 | 537 | 159 | 517 | 1.61e-45 | 172 |
| MsG0680032378.01.T01 | AT3G01540 | 29.861 | 432 | 220 | 13 | 116 | 537 | 159 | 517 | 1.61e-45 | 172 |
| MsG0680032378.01.T01 | AT3G01540 | 29.861 | 432 | 220 | 13 | 116 | 537 | 159 | 517 | 1.62e-45 | 172 |
| MsG0680032378.01.T01 | AT1G16280 | 27.944 | 501 | 284 | 10 | 116 | 610 | 59 | 488 | 1.82e-45 | 170 |
| MsG0680032378.01.T01 | AT3G18600 | 30.806 | 422 | 213 | 15 | 110 | 521 | 82 | 434 | 1.19e-44 | 169 |
Find 204 sgRNAs with CRISPR-Local
Find 577 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCTGCATACCTTTGTCA+TGG | 0.579225 | 6:-39672605 | None:intergenic |
| AAATCTGTGCAAACCAATGT+GGG | 0.527355 | 6:-39676211 | None:intergenic |
| AAATGTTTGTCTCTTATCTG+AGG | 0.575971 | 6:-39677354 | None:intergenic |
| AACAAGTTTGGTTACTAAAA+AGG | 0.214684 | 6:+39676785 | MsG0680032378.01.T01:CDS |
| AACCCCATTGATATGGTAGT+TGG | 0.483448 | 6:+39672304 | MsG0680032378.01.T01:CDS |
| AACTTAGTGTTGTCGGATTT+AGG | 0.207786 | 6:+39677315 | MsG0680032378.01.T01:CDS |
| AAGGGTGGTGAGGAAGGTTG+AGG | 0.415334 | 6:+39670061 | MsG0680032378.01.T01:CDS |
| AAGTCTTGCAAAGGGCAACA+GGG | 0.646188 | 6:+39674775 | MsG0680032378.01.T01:CDS |
| AAGTTTAGAACGTGTAGATT+TGG | 0.351497 | 6:-39677297 | None:intergenic |
| AATACCTGACCTGGTATTCA+TGG | 0.410412 | 6:-39676982 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAAGAACGAGAATTTGG+AGG | + | Chr6:39676842-39676861 | MsG0680032378.01.T01:CDS | 30.0% | |
| AAATGTTTGTCTCTTATCTG+AGG | - | Chr6:39677357-39677376 | None:intergenic | 30.0% | |
| AACTTAGAAGATTATTCGAG+GGG | + | Chr6:39672673-39672692 | MsG0680032378.01.T01:intron | 30.0% | |
| AATGAGACCCTAAATATGTA+AGG | + | Chr6:39673611-39673630 | MsG0680032378.01.T01:intron | 30.0% | |
| AATGTATGGAACAAGTTGTT+TGG | + | Chr6:39671732-39671751 | MsG0680032378.01.T01:intron | 30.0% | |
| AGCTGTTAGACTTCTTATTA+TGG | - | Chr6:39673949-39673968 | None:intergenic | 30.0% | |
| ATAACCCGTAAACAAAATTG+TGG | - | Chr6:39676588-39676607 | None:intergenic | 30.0% | |
| ATACATGCCTATACATTTAC+TGG | - | Chr6:39671978-39671997 | None:intergenic | 30.0% | |
| ATATCGCTATCCAAATAAAG+TGG | + | Chr6:39671603-39671622 | MsG0680032378.01.T01:intron | 30.0% | |
| ATTATCGGTGTAGAAAACAA+AGG | - | Chr6:39673145-39673164 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 39669798 | 39677379 | 39669798 | ID=MsG0680032378.01;Name=MsG0680032378.01 |
| Chr6 | mRNA | 39669798 | 39677379 | 39669798 | ID=MsG0680032378.01.T01;Parent=MsG0680032378.01;Name=MsG0680032378.01.T01;_AED=0.36;_eAED=0.41;_QI=0|0.66|0.5|1|1|1|10|0|719 |
| Chr6 | exon | 39669798 | 39670322 | 39669798 | ID=MsG0680032378.01.T01:exon:21595;Parent=MsG0680032378.01.T01 |
| Chr6 | exon | 39672032 | 39672127 | 39672032 | ID=MsG0680032378.01.T01:exon:21596;Parent=MsG0680032378.01.T01 |
| Chr6 | exon | 39672205 | 39672393 | 39672205 | ID=MsG0680032378.01.T01:exon:21597;Parent=MsG0680032378.01.T01 |
| Chr6 | exon | 39672475 | 39672615 | 39672475 | ID=MsG0680032378.01.T01:exon:21598;Parent=MsG0680032378.01.T01 |
| Chr6 | exon | 39673387 | 39673527 | 39673387 | ID=MsG0680032378.01.T01:exon:21599;Parent=MsG0680032378.01.T01 |
| Chr6 | exon | 39674764 | 39674889 | 39674764 | ID=MsG0680032378.01.T01:exon:21600;Parent=MsG0680032378.01.T01 |
| Chr6 | exon | 39675016 | 39675176 | 39675016 | ID=MsG0680032378.01.T01:exon:21601;Parent=MsG0680032378.01.T01 |
| Chr6 | exon | 39676168 | 39676297 | 39676168 | ID=MsG0680032378.01.T01:exon:21602;Parent=MsG0680032378.01.T01 |
| Chr6 | exon | 39676379 | 39676427 | 39676379 | ID=MsG0680032378.01.T01:exon:21603;Parent=MsG0680032378.01.T01 |
| Chr6 | exon | 39676778 | 39677379 | 39676778 | ID=MsG0680032378.01.T01:exon:21604;Parent=MsG0680032378.01.T01 |
| Chr6 | CDS | 39669798 | 39670322 | 39669798 | ID=MsG0680032378.01.T01:cds;Parent=MsG0680032378.01.T01 |
| Chr6 | CDS | 39672032 | 39672127 | 39672032 | ID=MsG0680032378.01.T01:cds;Parent=MsG0680032378.01.T01 |
| Chr6 | CDS | 39672205 | 39672393 | 39672205 | ID=MsG0680032378.01.T01:cds;Parent=MsG0680032378.01.T01 |
| Chr6 | CDS | 39672475 | 39672615 | 39672475 | ID=MsG0680032378.01.T01:cds;Parent=MsG0680032378.01.T01 |
| Chr6 | CDS | 39673387 | 39673527 | 39673387 | ID=MsG0680032378.01.T01:cds;Parent=MsG0680032378.01.T01 |
| Chr6 | CDS | 39674764 | 39674889 | 39674764 | ID=MsG0680032378.01.T01:cds;Parent=MsG0680032378.01.T01 |
| Chr6 | CDS | 39675016 | 39675176 | 39675016 | ID=MsG0680032378.01.T01:cds;Parent=MsG0680032378.01.T01 |
| Chr6 | CDS | 39676168 | 39676297 | 39676168 | ID=MsG0680032378.01.T01:cds;Parent=MsG0680032378.01.T01 |
| Chr6 | CDS | 39676379 | 39676427 | 39676379 | ID=MsG0680032378.01.T01:cds;Parent=MsG0680032378.01.T01 |
| Chr6 | CDS | 39676778 | 39677379 | 39676778 | ID=MsG0680032378.01.T01:cds;Parent=MsG0680032378.01.T01 |
| Gene Sequence |
>MsG0680032378.01.T01 ATGGCGGTTGCAACCAGAAAAACCCTCTTCACTCTCTCTTCCTCCATAACCTTCCCATCCCGTTTCTCTCTCTTCAAACTCTCCCAAAACCCATCATCCCTTTTCCCTAAACGCTTCACACCATTCTCTTCTCTCACCACCACATCACAATCCTCACCATCATTGCTATCTGACCCTGATCAAGAAAAACACTCTGTTCTTCTCGAGAAGTTGAGGATAAGACATTTGAAAGGTGGAAGTCGAAGTGGGAGTAGGAATGATGAAAGGGTGGTGAGGAAGGTTGAGGGGGTGAAGAAGAAGAAAGAGGGGGTGTTTGAGGAGGAGGATGGTGGTGGTGTTGTTGGGAGTTTTGAAGGGTTGGGATTAGGGGATGAGGTTATGGGGGCTGTTAGAGAAATGGGTATTGAAGTTCCTACTGAGATTCAGTGTATTGGTGTTCCGGCTGTTTTGGATGGGAAGAGTGTTGTGCTAGGGTCTCATACTGGGTCTGGGAAGACTCTTGCTTATTTGTTACCGCTTGTTCAGTTGCTAAGACGGGATGAGCAAATGAATGGACTAGTGTTGAAGCCCAAGCGTCCCCGAGCTGTTGTGCTATGCCCTACGAGGGAGTTGTCTGAGCAGGTCTTTCGAGTTGCAAAATCGATAAGCCATCATGCACGATTCAGGTGTACCATGGTAAGTGGTGGTGGCCGTCTTAGGCCTCAAGAGGAGTCACTTAGTAACCCCATTGATATGGTAGTTGGAACCCCTGGCAGGATTCTTCAACATATCGAGGAAGGAAATATGGTATACGGTGACATTCAATATGTGGTATTAGATGAGGCAGACACAATGTTTGATCGGGGCTTTGGTCCTGATATTAAAAAGTTCCTGGCCCCATTAAAACATCGCGCTTCAAAACCATACAGTCTAGGTTTCCAGACTGTTTTGGTAACTGCAACCATGACAAAGGCTGTGCAAAATCTGGTTGATGAGGAGTTTCAAGGCATTGTACATCTGCGCACATCCTCATTACATAAGAAGATTTCTAATGCTCGTCATGATTTTATCAAACTTGCTGGTACCGAGAACAAACTGGATTCATTACTTCAGGTATTAGAGCCAAGTCTTGCAAAGGGCAACAGGGTAATGGTTTTCTGCAACACATTGAATTCTAGTCGTGCTGTGGATCACTTTCTTGAAGAAAATCAGATATCTACCGTGAATTACCATGGGGAGGAAATAGCACTGTCAACTGTTGAAATTTTAGGGATTGGATGGAATAGGAATAATTTAGACTTAAAAGTGAATGATATGTTTTACTATCGATACTCAATAGTTGTGTTACAACTCACTGATTTTCTTTATGCCACTAAATCTATGGCTATGGAGAAATTCAGGGTTGAGAACCTCAACAAGTTCAAGAGTAACAACGATGATTGCCCCACATTGGTTTGCACAGATTTGGCTGCTAGGGGTCTGGACTTGGATGTTGATCATGTTATCATGTTTGACTTCCCTTTGAATTCGATTGACTACCTCCATCGCACCGGTAGAACTGCCCGTATGGGTGCAAAAGGGAAAATAACAAGTTTGGTTACTAAAAAGGACTATAGCTTGGCAACCAAAATAGAGGAGGCAATAAAAAAGAACGAGAATTTGGAGGCTATCACAAAAGAAAGTGTCCGAATGGATGTAACCAGGAACCAAATCACTGAGCAGAGAAGAAAGAACAAAAATGTGGTTAAACCTTCAAAAGTTAGGGACAAATCTGATTCTCATACCATGAATACCAGGTCAGGTATTAAGGACAAATCCGATTCTCGCAGAAGATCTGAGAATACCAGGTCAGGTACCAGGGACAAATCCGATTCTCGCAGCAGATCTGAGAATACCAGGTCAGGTACTAGGGACAAATCCGATTCTCGCAACAGATCTGGGAATGCCAGGTCAGGTACAAAATCGAGTAAACAATCAACGCCATCAAAGTTTACGAAGAAAGGATTTACCGTTTCAAAATCTGTAAAATCTTCCAATACAAGTAGCTTCAAGAAAACTTCTTCAGATAATAAGCGAACGGGTAAAAAAACCACTGCCTCCAAATCTACACGTTCTAAACTTAGTGTTGTCGGATTTAGGGGGCGGAATGCATCATCCTCAGATAAGAGACAAACATTTTAG |
| Protein sequence |
>MsG0680032378.01.T01 MAVATRKTLFTLSSSITFPSRFSLFKLSQNPSSLFPKRFTPFSSLTTTSQSSPSLLSDPDQEKHSVLLEKLRIRHLKGGSRSGSRNDERVVRKVEGVKKKKEGVFEEEDGGGVVGSFEGLGLGDEVMGAVREMGIEVPTEIQCIGVPAVLDGKSVVLGSHTGSGKTLAYLLPLVQLLRRDEQMNGLVLKPKRPRAVVLCPTRELSEQVFRVAKSISHHARFRCTMVSGGGRLRPQEESLSNPIDMVVGTPGRILQHIEEGNMVYGDIQYVVLDEADTMFDRGFGPDIKKFLAPLKHRASKPYSLGFQTVLVTATMTKAVQNLVDEEFQGIVHLRTSSLHKKISNARHDFIKLAGTENKLDSLLQVLEPSLAKGNRVMVFCNTLNSSRAVDHFLEENQISTVNYHGEEIALSTVEILGIGWNRNNLDLKVNDMFYYRYSIVVLQLTDFLYATKSMAMEKFRVENLNKFKSNNDDCPTLVCTDLAARGLDLDVDHVIMFDFPLNSIDYLHRTGRTARMGAKGKITSLVTKKDYSLATKIEEAIKKNENLEAITKESVRMDVTRNQITEQRRKNKNVVKPSKVRDKSDSHTMNTRSGIKDKSDSRRRSENTRSGTRDKSDSRSRSENTRSGTRDKSDSRNRSGNARSGTKSSKQSTPSKFTKKGFTVSKSVKSSNTSSFKKTSSDNKRTGKKTTASKSTRSKLSVVGFRGRNASSSDKRQTF* |
