AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042297.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042297.01.T01 MTR_8g009700 62.712 118 18 2 53 144 26 143 1.89e-40 137
MsG0880042297.01.T01 MTR_8g009680 49.123 171 54 6 2 144 15 180 1.86e-33 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042297.01.T01 AT5G17660 41.284 109 36 3 67 147 68 176 2.50e-15 72.0

Find 48 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCATAAGAGCTTGTTAAA+TGG 0.311368 8:+6689249 MsG0880042297.01.T01:three_prime_UTR
GTTCTGCTACTGCCACTACT+TGG 0.322586 8:-6685465 None:intergenic
TAAGCAACCCTATGGGGATT+AGG 0.323830 8:+6689051 MsG0880042297.01.T01:three_prime_UTR
CCCAAATGATTTCTCGCTTT+AGG 0.331633 8:-6687705 None:intergenic
ACCTAAAGCGAGAAATCATT+TGG 0.332484 8:+6687704 MsG0880042297.01.T01:CDS
GTGCATCAACTTCATCTAAC+TGG 0.350365 8:-6688973 None:intergenic
ATCATTTGGGATTGGAAATA+CGG 0.352445 8:+6687718 MsG0880042297.01.T01:CDS
AGAGATTGAAAGAGAAGAAT+AGG 0.359221 8:-6685419 None:intergenic
TAAGAGCTTGTTAAATGGTT+AGG 0.362323 8:+6689254 MsG0880042297.01.T01:three_prime_UTR
AGTCACAAGAAGAAGTTATA+TGG 0.366452 8:+6685319 MsG0880042297.01.T01:CDS
TATGTCATATTTCAGAATTT+GGG 0.367238 8:+6685675 MsG0880042297.01.T01:intron
TCTGATTATTCACTTGTATG+AGG 0.381902 8:+6689309 MsG0880042297.01.T01:three_prime_UTR
ATCAGAGTTCAAAGCATCTA+TGG 0.383728 8:-6689001 None:intergenic
ATTTGGGTCATGGCAGAATC+AGG 0.419446 8:+6685691 MsG0880042297.01.T01:CDS
CCTCAATCTCTCTTACAACT+TGG 0.429111 8:+6685549 MsG0880042297.01.T01:CDS
GGGATTGGAAATACGGCAAA+AGG 0.432298 8:+6687725 MsG0880042297.01.T01:CDS
TAGGAAGAAAGAGGACGAAA+GGG 0.442193 8:-6685400 None:intergenic
TTCTGCTACTGCCACTACTT+GGG 0.447482 8:-6685464 None:intergenic
AGTTAAGGTCGATGGGTTCA+TGG 0.448731 8:+6689188 MsG0880042297.01.T01:three_prime_UTR
AGCGAGAAATCATTTGGGAT+TGG 0.464188 8:+6687710 MsG0880042297.01.T01:CDS
TCTGTGTTATTGAGAAGTTA+AGG 0.467181 8:+6689173 MsG0880042297.01.T01:three_prime_UTR
CCTAAAGCGAGAAATCATTT+GGG 0.468102 8:+6687705 MsG0880042297.01.T01:CDS
TGTTAAATGGTTAGGTGGGT+AGG 0.468650 8:+6689262 MsG0880042297.01.T01:three_prime_UTR
GCAGCGGCAGGTTTCTTATG+TGG 0.479775 8:+6687667 MsG0880042297.01.T01:CDS
GTTAAATGGTTAGGTGGGTA+GGG 0.480224 8:+6689263 MsG0880042297.01.T01:three_prime_UTR
CTCTCAGTCCTAATCCCCAT+AGG 0.490442 8:-6689059 None:intergenic
TATTGAGAAGTTAAGGTCGA+TGG 0.491147 8:+6689180 MsG0880042297.01.T01:three_prime_UTR
ATAGGAAGAAAGAGGACGAA+AGG 0.491848 8:-6685401 None:intergenic
ATGGTTATTAAGCAACCCTA+TGG 0.492202 8:+6689043 MsG0880042297.01.T01:three_prime_UTR
TTCTTCTTTGTCCCAAGTAG+TGG 0.503579 8:+6685453 MsG0880042297.01.T01:CDS
AAATAATGTCAGGCAGCGGC+AGG 0.509305 8:+6687655 MsG0880042297.01.T01:intron
AAGCTCACAACTTGTTGCAT+TGG 0.510644 8:+6685516 MsG0880042297.01.T01:CDS
AGCTTGTTAAATGGTTAGGT+GGG 0.512979 8:+6689258 MsG0880042297.01.T01:three_prime_UTR
AGTTGTGAGCTTCGTAATTG+TGG 0.514100 8:-6685505 None:intergenic
CCAAGTTGTAAGAGAGATTG+AGG 0.515832 8:-6685549 None:intergenic
GAGCTTGTTAAATGGTTAGG+TGG 0.523946 8:+6689257 MsG0880042297.01.T01:three_prime_UTR
ATTGAGAAGTTAAGGTCGAT+GGG 0.533782 8:+6689181 MsG0880042297.01.T01:three_prime_UTR
TGGTTATTAAGCAACCCTAT+GGG 0.534053 8:+6689044 MsG0880042297.01.T01:three_prime_UTR
GAACATACAGAGAAAGAAGA+AGG 0.534792 8:-6685726 None:intergenic
AGAGAAGAATAGGAAGAAAG+AGG 0.538610 8:-6685409 None:intergenic
TCTCAGTCCTAATCCCCATA+GGG 0.549156 8:-6689058 None:intergenic
ACAATCTGATGCACTTGAAG+TGG 0.556037 8:+6688934 MsG0880042297.01.T01:CDS
ATATTTCAGAATTTGGGTCA+TGG 0.598590 8:+6685681 MsG0880042297.01.T01:intron
GAGATTCACGCAGAATTCGA+AGG 0.613031 8:+6689083 MsG0880042297.01.T01:three_prime_UTR
ATTTGTGTGACAGTGAAGGA+TGG 0.614493 8:+6689024 MsG0880042297.01.T01:three_prime_UTR
GGTTATTAAGCAACCCTATG+GGG 0.641395 8:+6689045 MsG0880042297.01.T01:three_prime_UTR
TGAGAGTGTTAACATTAGTG+CGG 0.687588 8:-6685365 None:intergenic
TGATATTTGTGTGACAGTGA+AGG 0.688796 8:+6689020 MsG0880042297.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATGATAATTATAGTAT+AGG - Chr8:6685825-6685844 None:intergenic 10.0%
!! ATGTTTATATAATATAATGA+AGG - Chr8:6686784-6686803 None:intergenic 10.0%
!! TAAATGATAATTATAGTATA+GGG - Chr8:6685824-6685843 None:intergenic 10.0%
!!! ATGATATTTTTTCTTTAAAT+GGG - Chr8:6688486-6688505 None:intergenic 10.0%
!!! TATGATATTTTTTCTTTAAA+TGG - Chr8:6688487-6688506 None:intergenic 10.0%
!! AATTATTTAGATTTAAAGAG+GGG + Chr8:6686290-6686309 MsG0880042297.01.T01:intron 15.0%
!! ACTAAAACAACAAATTTATA+TGG + Chr8:6686739-6686758 MsG0880042297.01.T01:intron 15.0%
!! CAATTATTTAGATTTAAAGA+GGG + Chr8:6686289-6686308 MsG0880042297.01.T01:intron 15.0%
!! TCAATATATCATTCATAAAA+TGG - Chr8:6686937-6686956 None:intergenic 15.0%
!! TTAACTCATAATCATATTTA+TGG + Chr8:6688816-6688835 MsG0880042297.01.T01:intron 15.0%
!! TTGTATGAAATATATACATT+TGG + Chr8:6686805-6686824 MsG0880042297.01.T01:intron 15.0%
!!! AATCATAAACTTTATTTTGA+CGG + Chr8:6687958-6687977 MsG0880042297.01.T01:intron 15.0%
!!! TGATATTTTTTCTTTAAATG+GGG - Chr8:6688485-6688504 None:intergenic 15.0%
!! AAAAAACAGGATTTCAAAAA+GGG + Chr8:6686554-6686573 MsG0880042297.01.T01:intron 20.0%
!! AAGGTGAATTTATAATATAC+AGG - Chr8:6686447-6686466 None:intergenic 20.0%
!! AGTAAATTGCACAATATTTA+TGG - Chr8:6688612-6688631 None:intergenic 20.0%
!! ATATGAGAAAACATTTGAAT+TGG - Chr8:6685766-6685785 None:intergenic 20.0%
!! ATTATATTATCCGTTTAAAG+TGG - Chr8:6685608-6685627 None:intergenic 20.0%
!! ATTATTTAGATTTAAAGAGG+GGG + Chr8:6686291-6686310 MsG0880042297.01.T01:intron 20.0%
!! CAAGAAAAAAATATCATTAC+AGG - Chr8:6687051-6687070 None:intergenic 20.0%
!! CCAATTATTTAGATTTAAAG+AGG + Chr8:6686288-6686307 MsG0880042297.01.T01:intron 20.0%
!! CCTCTTTAAATCTAAATAAT+TGG - Chr8:6686291-6686310 None:intergenic 20.0%
!! TACACTAATAGTAAGAAAAA+TGG - Chr8:6688722-6688741 None:intergenic 20.0%
!! TATAGGTACTAAATACAAAA+CGG - Chr8:6686629-6686648 None:intergenic 20.0%
!! TATCAACTGCTTAAAAAATT+GGG - Chr8:6688075-6688094 None:intergenic 20.0%
!! TATGTCATATTTCAGAATTT+GGG + Chr8:6685675-6685694 MsG0880042297.01.T01:intron 20.0%
!! TTACTATTAGTGTAAATGTA+TGG + Chr8:6688728-6688747 MsG0880042297.01.T01:intron 20.0%
!! TTAGCATACATATTAATGAT+TGG + Chr8:6687217-6687236 MsG0880042297.01.T01:intron 20.0%
!! TTATGTCATATTTCAGAATT+TGG + Chr8:6685674-6685693 MsG0880042297.01.T01:intron 20.0%
!! TTCATTAGCAAATCAAAATA+GGG - Chr8:6685879-6685898 None:intergenic 20.0%
!! TTTCATTAGCAAATCAAAAT+AGG - Chr8:6685880-6685899 None:intergenic 20.0%
!!! AATGAACATGTTTTAAAAAG+GGG - Chr8:6685908-6685927 None:intergenic 20.0%
!!! GAATGAACATGTTTTAAAAA+GGG - Chr8:6685909-6685928 None:intergenic 20.0%
!!! TAAAAGCATAAAGAGAATTT+GGG + Chr8:6688145-6688164 MsG0880042297.01.T01:intron 20.0%
!!! TCCTAAGATGTATTTTTTAT+CGG - Chr8:6689349-6689368 None:intergenic 20.0%
!!! TGTTTTCTCATATTCATTAT+AGG + Chr8:6685773-6685792 MsG0880042297.01.T01:intron 20.0%
! AAAAACAGGATTTCAAAAAG+GGG + Chr8:6686555-6686574 MsG0880042297.01.T01:intron 25.0%
! ACATGATCTATTATTGAGTT+TGG + Chr8:6687279-6687298 MsG0880042297.01.T01:intron 25.0%
! AGAAATCAAAATTCACTGAA+AGG - Chr8:6687802-6687821 None:intergenic 25.0%
! AGGCATGAAATATCATTAAA+AGG - Chr8:6688853-6688872 None:intergenic 25.0%
! AGTGGAAAATGAAAGTAAAA+AGG - Chr8:6685590-6685609 None:intergenic 25.0%
! ATACAAAATCTTATGTACCA+GGG - Chr8:6688696-6688715 None:intergenic 25.0%
! ATCAACTGCTTAAAAAATTG+GGG - Chr8:6688074-6688093 None:intergenic 25.0%
! CAAAAAACAGGATTTCAAAA+AGG + Chr8:6686553-6686572 MsG0880042297.01.T01:intron 25.0%
! CTATCAACTGCTTAAAAAAT+TGG - Chr8:6688076-6688095 None:intergenic 25.0%
! GAAATCAAAATTCACTGAAA+GGG - Chr8:6687801-6687820 None:intergenic 25.0%
! GGCATGAAATATCATTAAAA+GGG - Chr8:6688852-6688871 None:intergenic 25.0%
! GTGAATTTATAATATACAGG+TGG - Chr8:6686444-6686463 None:intergenic 25.0%
! TACAAACACAAATTCAAATG+TGG + Chr8:6685954-6685973 MsG0880042297.01.T01:intron 25.0%
! TACATATTAATGATTGGCAT+TGG + Chr8:6687223-6687242 MsG0880042297.01.T01:intron 25.0%
! TATTGTAGAAATTTATCTCC+CGG + Chr8:6688540-6688559 MsG0880042297.01.T01:intron 25.0%
! TCCGATAAAAAATACATCTT+AGG + Chr8:6689345-6689364 MsG0880042297.01.T01:three_prime_UTR 25.0%
!! ACTTTGATTACAAGATGTTT+TGG - Chr8:6687330-6687349 None:intergenic 25.0%
!! ATTTTCAACAACAGATATAG+TGG - Chr8:6687639-6687658 None:intergenic 25.0%
!! CTAAAAGCATAAAGAGAATT+TGG + Chr8:6688144-6688163 MsG0880042297.01.T01:intron 25.0%
!! CTTTCATTTTCCACTTTAAA+CGG + Chr8:6685595-6685614 MsG0880042297.01.T01:intron 25.0%
!! GATTTTATCACATTATTCGT+AGG - Chr8:6686051-6686070 None:intergenic 25.0%
!! TGATTGAAGTTGATCTATTA+AGG + Chr8:6686147-6686166 MsG0880042297.01.T01:intron 25.0%
!! TGTTTTGTACAGATTGAAAA+GGG - Chr8:6686652-6686671 None:intergenic 25.0%
!!! CGAATGAACATGTTTTAAAA+AGG - Chr8:6685910-6685929 None:intergenic 25.0%
!!! CTTTTTGAAATCCTGTTTTT+TGG - Chr8:6686555-6686574 None:intergenic 25.0%
!!! GTTTTGTTTGTCTTAATTGT+AGG + Chr8:6685850-6685869 MsG0880042297.01.T01:intron 25.0%
!!! TTGTTTTGTACAGATTGAAA+AGG - Chr8:6686653-6686672 None:intergenic 25.0%
!!! TTTCATAAGAGCTTGTTAAA+TGG + Chr8:6689249-6689268 MsG0880042297.01.T01:three_prime_UTR 25.0%
!!! TTTTGATCTCTTATTTTCAC+TGG - Chr8:6686595-6686614 None:intergenic 25.0%
AATCAAATGAAAACGCACAT+AGG + Chr8:6686183-6686202 MsG0880042297.01.T01:intron 30.0%
ACAAGATATGGCTGATATTT+AGG + Chr8:6688655-6688674 MsG0880042297.01.T01:intron 30.0%
ACATGTTCATTCGTTGAAAT+TGG + Chr8:6685917-6685936 MsG0880042297.01.T01:intron 30.0%
AGAGATTGAAAGAGAAGAAT+AGG - Chr8:6685422-6685441 None:intergenic 30.0%
AGTCACAAGAAGAAGTTATA+TGG + Chr8:6685319-6685338 MsG0880042297.01.T01:CDS 30.0%
ATATTTCAGAATTTGGGTCA+TGG + Chr8:6685681-6685700 MsG0880042297.01.T01:intron 30.0%
ATTGTAGAAATTTATCTCCC+GGG + Chr8:6688541-6688560 MsG0880042297.01.T01:intron 30.0%
CATAAAACACGACCATAAAA+TGG - Chr8:6688902-6688921 None:intergenic 30.0%
CATACAAAATCTTATGTACC+AGG - Chr8:6688697-6688716 None:intergenic 30.0%
CGTACATAATGAAAGACAAA+AGG - Chr8:6687013-6687032 None:intergenic 30.0%
CTGTTGTTGAAAATAATGTC+AGG + Chr8:6687645-6687664 MsG0880042297.01.T01:intron 30.0%
GACTTAATAGTAAGAACACT+GGG + Chr8:6686716-6686735 MsG0880042297.01.T01:intron 30.0%
GCTGCAATAACTTCTATTTA+AGG - Chr8:6687492-6687511 None:intergenic 30.0%
TAACAGCATAAAACGACAAA+AGG + Chr8:6686025-6686044 MsG0880042297.01.T01:intron 30.0%
TCTGATTAGATATCTCTGTA+AGG - Chr8:6686894-6686913 None:intergenic 30.0%
TCTGATTATTCACTTGTATG+AGG + Chr8:6689309-6689328 MsG0880042297.01.T01:three_prime_UTR 30.0%
TCTGCAATTAAGCGAAAAAA+CGG - Chr8:6688032-6688051 None:intergenic 30.0%
TGAATTGGTGTAGTAAAGAA+AGG - Chr8:6685751-6685770 None:intergenic 30.0%
TTAGAACCTCTTAAAGAATG+CGG + Chr8:6686684-6686703 MsG0880042297.01.T01:intron 30.0%
TTCTGCATGCTCTAATATAT+AGG + Chr8:6688577-6688596 MsG0880042297.01.T01:intron 30.0%
TTGTCTTTCATTATGTACGA+GGG + Chr8:6687014-6687033 MsG0880042297.01.T01:intron 30.0%
TTTGTCTTTCATTATGTACG+AGG + Chr8:6687013-6687032 MsG0880042297.01.T01:intron 30.0%
! ATATGGAGACATCTGTATTT+TGG - Chr8:6686081-6686100 None:intergenic 30.0%
! ATCATTTGGGATTGGAAATA+CGG + Chr8:6687718-6687737 MsG0880042297.01.T01:CDS 30.0%
! GCTACATTGTTGTTACTTAA+AGG + Chr8:6688399-6688418 MsG0880042297.01.T01:intron 30.0%
! TCTGTGTTATTGAGAAGTTA+AGG + Chr8:6689173-6689192 MsG0880042297.01.T01:three_prime_UTR 30.0%
! TGTCAAGTTTTTATTGACGT+GGG - Chr8:6686496-6686515 None:intergenic 30.0%
! TTTGGCTCAATATATCAGAA+AGG - Chr8:6687312-6687331 None:intergenic 30.0%
!! TAAGAGCTTGTTAAATGGTT+AGG + Chr8:6689254-6689273 MsG0880042297.01.T01:three_prime_UTR 30.0%
!!! ACTTGACACATCACATTTTT+TGG + Chr8:6686508-6686527 MsG0880042297.01.T01:intron 30.0%
!!! TTAAAGAATGCGGTTTTGAA+AGG + Chr8:6686694-6686713 MsG0880042297.01.T01:intron 30.0%
!!! TTTTTTCTTTAAATGGGGTC+TGG - Chr8:6688480-6688499 None:intergenic 30.0%
AAGGAGCAAAAATCTATCGA+AGG + Chr8:6689102-6689121 MsG0880042297.01.T01:three_prime_UTR 35.0%
AATGTAGCCAACAAGTGATT+TGG - Chr8:6688387-6688406 None:intergenic 35.0%
ACCTAAAGCGAGAAATCATT+TGG + Chr8:6687704-6687723 MsG0880042297.01.T01:CDS 35.0%
ACGGCAAAGTAAATGTATAC+AGG + Chr8:6687977-6687996 MsG0880042297.01.T01:intron 35.0%
AGAAATGAAACTGAGAGAGA+AGG + Chr8:6686372-6686391 MsG0880042297.01.T01:intron 35.0%
AGAGAAGAATAGGAAGAAAG+AGG - Chr8:6685412-6685431 None:intergenic 35.0%
ATAAAAAAACGAGCCCTTTC+CGG + Chr8:6686339-6686358 MsG0880042297.01.T01:intron 35.0%
ATCTATTAAGGAACCAAGAC+GGG + Chr8:6686159-6686178 MsG0880042297.01.T01:intron 35.0%
ATGGTTATTAAGCAACCCTA+TGG + Chr8:6689043-6689062 MsG0880042297.01.T01:three_prime_UTR 35.0%
ATTGAGAAGTTAAGGTCGAT+GGG + Chr8:6689181-6689200 MsG0880042297.01.T01:three_prime_UTR 35.0%
CAAAATTCACTGAAAGGGAA+GGG - Chr8:6687796-6687815 None:intergenic 35.0%
CACTTTCAGCTTAGAATAGA+GGG + Chr8:6687834-6687853 MsG0880042297.01.T01:intron 35.0%
CCTAAAGCGAGAAATCATTT+GGG + Chr8:6687705-6687724 MsG0880042297.01.T01:CDS 35.0%
GAAATCGAAACTAACTGCAA+GGG - Chr8:6688113-6688132 None:intergenic 35.0%
GAACATACAGAGAAAGAAGA+AGG - Chr8:6685729-6685748 None:intergenic 35.0%
GATCTATTAAGGAACCAAGA+CGG + Chr8:6686158-6686177 MsG0880042297.01.T01:intron 35.0%
GATGAACCAAATACAGAAAC+CGG + Chr8:6686960-6686979 MsG0880042297.01.T01:intron 35.0%
GGACTTAATAGTAAGAACAC+TGG + Chr8:6686715-6686734 MsG0880042297.01.T01:intron 35.0%
TATTGAGAAGTTAAGGTCGA+TGG + Chr8:6689180-6689199 MsG0880042297.01.T01:three_prime_UTR 35.0%
TCAAAACCGCATTCTTTAAG+AGG - Chr8:6686693-6686712 None:intergenic 35.0%
TCAAAATTCACTGAAAGGGA+AGG - Chr8:6687797-6687816 None:intergenic 35.0%
TGAGTTATGGGTAAAAGATC+TGG + Chr8:6687914-6687933 MsG0880042297.01.T01:intron 35.0%
TGATATTTGTGTGACAGTGA+AGG + Chr8:6689020-6689039 MsG0880042297.01.T01:three_prime_UTR 35.0%
TGGTTATTAAGCAACCCTAT+GGG + Chr8:6689044-6689063 MsG0880042297.01.T01:three_prime_UTR 35.0%
TTGAAAATAATGTCAGGCAG+CGG + Chr8:6687651-6687670 MsG0880042297.01.T01:intron 35.0%
TTGTAGAAATTTATCTCCCG+GGG + Chr8:6688542-6688561 MsG0880042297.01.T01:intron 35.0%
! AACTCTTTTACCTCAACATC+AGG + Chr8:6687366-6687385 MsG0880042297.01.T01:intron 35.0%
! ATCAGAGTTCAAAGCATCTA+TGG - Chr8:6689004-6689023 None:intergenic 35.0%
! CTTGCAGTTAGTTTCGATTT+CGG + Chr8:6688112-6688131 MsG0880042297.01.T01:intron 35.0%
! GAAGAGTTTTGCAAAAGCTT+TGG + Chr8:6688510-6688529 MsG0880042297.01.T01:intron 35.0%
! GTACAGATTGAAAAGGGTAT+AGG - Chr8:6686646-6686665 None:intergenic 35.0%
! GTGTCAAGTTTTTATTGACG+TGG - Chr8:6686497-6686516 None:intergenic 35.0%
! TAGTAGACAACATTAGCTCT+TGG + Chr8:6687565-6687584 MsG0880042297.01.T01:intron 35.0%
! TTATGGTCGTGTTTTATGAC+AGG + Chr8:6688904-6688923 MsG0880042297.01.T01:intron 35.0%
! TTTTACCTCAACATCAGGAA+GGG + Chr8:6687371-6687390 MsG0880042297.01.T01:intron 35.0%
!! AGCTTGTTAAATGGTTAGGT+GGG + Chr8:6689258-6689277 MsG0880042297.01.T01:three_prime_UTR 35.0%
!! AGTTTTGCAAAAGCTTTGGT+CGG + Chr8:6688514-6688533 MsG0880042297.01.T01:intron 35.0%
!! ATTAATGATTGGCATTGGCT+TGG + Chr8:6687228-6687247 MsG0880042297.01.T01:intron 35.0%
!! CAGTTGATAGAATCATATCC+TGG + Chr8:6688086-6688105 MsG0880042297.01.T01:intron 35.0%
!! GAGAGTCGTTTTCCATTTTA+TGG + Chr8:6688887-6688906 MsG0880042297.01.T01:intron 35.0%
!! GTAATTGTGGCTTTTGTTGT+TGG - Chr8:6685495-6685514 None:intergenic 35.0%
!! GTCGTTTTATGCTGTTAACT+TGG - Chr8:6686022-6686041 None:intergenic 35.0%
!! TGAGAGTGTTAACATTAGTG+CGG - Chr8:6685368-6685387 None:intergenic 35.0%
!!! AAAGGGCTCGTTTTTTTATC+CGG - Chr8:6686338-6686357 None:intergenic 35.0%
AAGATGCACAGAAGTGATTC+CGG + Chr8:6686316-6686335 MsG0880042297.01.T01:intron 40.0%
ACAATCTGATGCACTTGAAG+TGG + Chr8:6688934-6688953 MsG0880042297.01.T01:CDS 40.0%
ACTACCACGTGTACAAGATA+TGG + Chr8:6688643-6688662 MsG0880042297.01.T01:intron 40.0%
ACTGTGTGGAATTATGAGTG+CGG + Chr8:6688356-6688375 MsG0880042297.01.T01:intron 40.0%
ACTTCAATTCCATGTGCAGA+TGG + Chr8:6687881-6687900 MsG0880042297.01.T01:intron 40.0%
AGTTGTGAGCTTCGTAATTG+TGG - Chr8:6685508-6685527 None:intergenic 40.0%
ATACAGAAACCGGACCTAAT+AGG + Chr8:6686970-6686989 MsG0880042297.01.T01:intron 40.0%
ATAGGAAGAAAGAGGACGAA+AGG - Chr8:6685404-6685423 None:intergenic 40.0%
ATTAACCCTTCCTGATGTTG+AGG - Chr8:6687379-6687398 None:intergenic 40.0%
ATTCAGCACATCGGTAACAT+TGG + Chr8:6688235-6688254 MsG0880042297.01.T01:intron 40.0%
ATTTGTGTGACAGTGAAGGA+TGG + Chr8:6689024-6689043 MsG0880042297.01.T01:three_prime_UTR 40.0%
CAATAATCTGAGTGAGTGAG+TGG - Chr8:6686410-6686429 None:intergenic 40.0%
CACTCACTCACTCAGATTAT+TGG + Chr8:6686408-6686427 MsG0880042297.01.T01:intron 40.0%
CATCCAATATCTACCATGAG+TGG - Chr8:6687129-6687148 None:intergenic 40.0%
CATTCACGTATTCAGCACAT+CGG + Chr8:6688226-6688245 MsG0880042297.01.T01:intron 40.0%
CCAAGTTGTAAGAGAGATTG+AGG - Chr8:6685552-6685571 None:intergenic 40.0%
CCCAAATGATTTCTCGCTTT+AGG - Chr8:6687708-6687727 None:intergenic 40.0%
CCTCAATCTCTCTTACAACT+TGG + Chr8:6685549-6685568 MsG0880042297.01.T01:CDS 40.0%
CGAAATCGAAACTAACTGCA+AGG - Chr8:6688114-6688133 None:intergenic 40.0%
CGGTAACATTGGATTGAGAT+CGG + Chr8:6688246-6688265 MsG0880042297.01.T01:intron 40.0%
CTCAACCAATAGCAATGACT+AGG - Chr8:6686119-6686138 None:intergenic 40.0%
CTCATGGTAGATATTGGATG+TGG + Chr8:6687129-6687148 MsG0880042297.01.T01:intron 40.0%
CTCTGTAAGGTGACAAAAGA+TGG - Chr8:6686881-6686900 None:intergenic 40.0%
GCACTTTCAGCTTAGAATAG+AGG + Chr8:6687833-6687852 MsG0880042297.01.T01:intron 40.0%
GGGAAATCCAAATCACTTGT+TGG + Chr8:6688377-6688396 MsG0880042297.01.T01:intron 40.0%
GGTTATTAAGCAACCCTATG+GGG + Chr8:6689045-6689064 MsG0880042297.01.T01:three_prime_UTR 40.0%
GTGATTTCGTCGTATTAGTG+TGG - Chr8:6686226-6686245 None:intergenic 40.0%
GTGCATCAACTTCATCTAAC+TGG - Chr8:6688976-6688995 None:intergenic 40.0%
GTTAAATGGTTAGGTGGGTA+GGG + Chr8:6689263-6689282 MsG0880042297.01.T01:three_prime_UTR 40.0%
TAGGAAGAAAGAGGACGAAA+GGG - Chr8:6685403-6685422 None:intergenic 40.0%
TCTATTAAGGAACCAAGACG+GGG + Chr8:6686160-6686179 MsG0880042297.01.T01:intron 40.0%
TGAAAGACAAAAGGATTCGC+TGG - Chr8:6687004-6687023 None:intergenic 40.0%
TGGAAATACGGCAAAAGGTA+AGG + Chr8:6687730-6687749 MsG0880042297.01.T01:intron 40.0%
TGTAGAAATTTATCTCCCGG+GGG + Chr8:6688543-6688562 MsG0880042297.01.T01:intron 40.0%
TGTTAAATGGTTAGGTGGGT+AGG + Chr8:6689262-6689281 MsG0880042297.01.T01:three_prime_UTR 40.0%
TTAGGTCCGGTTTCTGTATT+TGG - Chr8:6686969-6686988 None:intergenic 40.0%
TTCATTTCTTCGTCATCCAC+CGG - Chr8:6686361-6686380 None:intergenic 40.0%
TTCTTCTTTGTCCCAAGTAG+TGG + Chr8:6685453-6685472 MsG0880042297.01.T01:CDS 40.0%
TTGTTCACGTGGAATCATCA+AGG - Chr8:6686466-6686485 None:intergenic 40.0%
TTTCATTTGATTCCCCGTCT+TGG - Chr8:6686175-6686194 None:intergenic 40.0%
! AGCGAGAAATCATTTGGGAT+TGG + Chr8:6687710-6687729 MsG0880042297.01.T01:CDS 40.0%
! CTTTTACCTCAACATCAGGA+AGG + Chr8:6687370-6687389 MsG0880042297.01.T01:intron 40.0%
!! AAGCTCACAACTTGTTGCAT+TGG + Chr8:6685516-6685535 MsG0880042297.01.T01:CDS 40.0%
!! GAGCTTGTTAAATGGTTAGG+TGG + Chr8:6689257-6689276 MsG0880042297.01.T01:three_prime_UTR 40.0%
AACCGGACCTAATAGGCATT+TGG + Chr8:6686977-6686996 MsG0880042297.01.T01:intron 45.0%
AAGGGAAGGGAGTTATCATC+AGG - Chr8:6687783-6687802 None:intergenic 45.0%
AAGTCCAGCCAATAGCCATT+AGG - Chr8:6688180-6688199 None:intergenic 45.0%
ACTCTCTTAAGTCTTCTCGC+AGG - Chr8:6688873-6688892 None:intergenic 45.0%
AGAAAATATACCTGTCCCCC+GGG - Chr8:6688561-6688580 None:intergenic 45.0%
AGGGAAGGGAGTTATCATCA+GGG - Chr8:6687782-6687801 None:intergenic 45.0%
ATGCGTGGAATTGTGACTTC+AGG - Chr8:6688209-6688228 None:intergenic 45.0%
ATTTGGGTCATGGCAGAATC+AGG + Chr8:6685691-6685710 MsG0880042297.01.T01:CDS 45.0%
CAGAAAATATACCTGTCCCC+CGG - Chr8:6688562-6688581 None:intergenic 45.0%
CTGTGTGGAATTATGAGTGC+GGG + Chr8:6688357-6688376 MsG0880042297.01.T01:intron 45.0%
GACCAAATGCCTATTAGGTC+CGG - Chr8:6686982-6687001 None:intergenic 45.0%
GACGACCTAGTCATTGCTAT+TGG + Chr8:6686111-6686130 MsG0880042297.01.T01:intron 45.0%
GAGATTCACGCAGAATTCGA+AGG + Chr8:6689083-6689102 MsG0880042297.01.T01:three_prime_UTR 45.0%
GGGATTGGAAATACGGCAAA+AGG + Chr8:6687725-6687744 MsG0880042297.01.T01:CDS 45.0%
GTCAACACATCAGTGGTTGT+TGG - Chr8:6688338-6688357 None:intergenic 45.0%
TAAGCAACCCTATGGGGATT+AGG + Chr8:6689051-6689070 MsG0880042297.01.T01:three_prime_UTR 45.0%
TAATCTGAGTGAGTGAGTGG+AGG - Chr8:6686407-6686426 None:intergenic 45.0%
TCAGCCATATCTTGTACACG+TGG - Chr8:6688650-6688669 None:intergenic 45.0%
TCGCCACTCATGGTAGATAT+TGG + Chr8:6687123-6687142 MsG0880042297.01.T01:intron 45.0%
TCTCAGTCCTAATCCCCATA+GGG - Chr8:6689061-6689080 None:intergenic 45.0%
TGAGCGATGGACCAAAAAAC+AGG + Chr8:6686541-6686560 MsG0880042297.01.T01:intron 45.0%
TTCTGCTACTGCCACTACTT+GGG - Chr8:6685467-6685486 None:intergenic 45.0%
! AGTTAAGGTCGATGGGTTCA+TGG + Chr8:6689188-6689207 MsG0880042297.01.T01:three_prime_UTR 45.0%
! ATGGGTAAAAGATCTGGCTC+TGG + Chr8:6687920-6687939 MsG0880042297.01.T01:intron 45.0%
! GGACTCTATATTTCTGTCCC+TGG + Chr8:6688676-6688695 MsG0880042297.01.T01:intron 45.0%
! TAATGGCTATTGGCTGGACT+TGG + Chr8:6688179-6688198 MsG0880042297.01.T01:intron 45.0%
! TGGGATCAAACTTGTTCACG+TGG - Chr8:6686477-6686496 None:intergenic 45.0%
AAAAAACGAGCCCTTTCCGG+TGG + Chr8:6686342-6686361 MsG0880042297.01.T01:intron 50.0%
AAATAATGTCAGGCAGCGGC+AGG + Chr8:6687655-6687674 MsG0880042297.01.T01:intron 50.0%
AAATTTATCTCCCGGGGGAC+AGG + Chr8:6688548-6688567 MsG0880042297.01.T01:intron 50.0%
ACAAGTACCAGACTGGAACC+AGG + Chr8:6687083-6687102 MsG0880042297.01.T01:intron 50.0%
ACGCTATAGCTGCTGCTCTT+TGG - Chr8:6687416-6687435 None:intergenic 50.0%
CCACACAGTCAACACATCAG+TGG - Chr8:6688345-6688364 None:intergenic 50.0%
CGCTGGACCAAATGCCTATT+AGG - Chr8:6686987-6687006 None:intergenic 50.0%
CTCTCAGTCCTAATCCCCAT+AGG - Chr8:6689062-6689081 None:intergenic 50.0%
CTGACCTAATGGCTATTGGC+TGG + Chr8:6688173-6688192 MsG0880042297.01.T01:intron 50.0%
CTGGACACTGCATGTGTTGA+GGG - Chr8:6688461-6688480 None:intergenic 50.0%
GAAACTAACTGCAAGGGACC+AGG - Chr8:6688107-6688126 None:intergenic 50.0%
GCCAATAGCCATTAGGTCAG+TGG - Chr8:6688173-6688192 None:intergenic 50.0%
GCCACTGACCTAATGGCTAT+TGG + Chr8:6688169-6688188 MsG0880042297.01.T01:intron 50.0%
GGTCAAGCGTGCTGAGTTAT+GGG + Chr8:6687902-6687921 MsG0880042297.01.T01:intron 50.0%
GGTCGTCAACGTTGCAGATA+TGG - Chr8:6686098-6686117 None:intergenic 50.0%
GTGCTGAATACGTGAATGCG+TGG - Chr8:6688224-6688243 None:intergenic 50.0%
GTTCTGCTACTGCCACTACT+TGG - Chr8:6685468-6685487 None:intergenic 50.0%
TCTGGACACTGCATGTGTTG+AGG - Chr8:6688462-6688481 None:intergenic 50.0%
TCTTCGTCATCCACCGGAAA+GGG - Chr8:6686355-6686374 None:intergenic 50.0%
TGGTCAAGCGTGCTGAGTTA+TGG + Chr8:6687901-6687920 MsG0880042297.01.T01:intron 50.0%
TGGTCCAGAAATCTGAGCGA+TGG + Chr8:6686528-6686547 MsG0880042297.01.T01:intron 50.0%
TGGTCCATCGCTCAGATTTC+TGG - Chr8:6686535-6686554 None:intergenic 50.0%
TTCCAGTCTGGTACTTGTGC+AGG - Chr8:6687081-6687100 None:intergenic 50.0%
TTCTTCGTCATCCACCGGAA+AGG - Chr8:6686356-6686375 None:intergenic 50.0%
!! CCACTGATGTGTTGACTGTG+TGG + Chr8:6688342-6688361 MsG0880042297.01.T01:intron 50.0%
!! TTTGGGTGCCACTGACCTAA+TGG + Chr8:6688162-6688181 MsG0880042297.01.T01:intron 50.0%
ATGCAGGGTCTGCAAAGACC+TGG - Chr8:6687104-6687123 None:intergenic 55.0%
CACGCTTGACCATCTGCACA+TGG - Chr8:6687893-6687912 None:intergenic 55.0%
CTACCATGAGTGGCGATGCA+GGG - Chr8:6687119-6687138 None:intergenic 55.0%
CTCCTGCACAAGTACCAGAC+TGG + Chr8:6687076-6687095 MsG0880042297.01.T01:intron 55.0%
GCAAAGACCTGGTTCCAGTC+TGG - Chr8:6687093-6687112 None:intergenic 55.0%
GCAGCGGCAGGTTTCTTATG+TGG + Chr8:6687667-6687686 MsG0880042297.01.T01:CDS 55.0%
GCTGCTGCTCTTTGGCAACA+CGG - Chr8:6687408-6687427 None:intergenic 55.0%
TCTACCATGAGTGGCGATGC+AGG - Chr8:6687120-6687139 None:intergenic 55.0%
AGACCCTGCATCGCCACTCA+TGG + Chr8:6687113-6687132 MsG0880042297.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 6685313 6689417 6685313 ID=MsG0880042297.01;Name=MsG0880042297.01
Chr8 mRNA 6685313 6689417 6685313 ID=MsG0880042297.01.T01;Parent=MsG0880042297.01;Name=MsG0880042297.01.T01;_AED=0.46;_eAED=0.48;_QI=0|0.33|0.5|1|0|0|4|411|157
Chr8 exon 6685313 6685570 6685313 ID=MsG0880042297.01.T01:exon:1156;Parent=MsG0880042297.01.T01
Chr8 exon 6685690 6685744 6685690 ID=MsG0880042297.01.T01:exon:1157;Parent=MsG0880042297.01.T01
Chr8 exon 6687667 6687746 6687667 ID=MsG0880042297.01.T01:exon:1158;Parent=MsG0880042297.01.T01
Chr8 exon 6688926 6689417 6688926 ID=MsG0880042297.01.T01:exon:1159;Parent=MsG0880042297.01.T01
Chr8 CDS 6685313 6685570 6685313 ID=MsG0880042297.01.T01:cds;Parent=MsG0880042297.01.T01
Chr8 CDS 6685690 6685744 6685690 ID=MsG0880042297.01.T01:cds;Parent=MsG0880042297.01.T01
Chr8 CDS 6687667 6687746 6687667 ID=MsG0880042297.01.T01:cds;Parent=MsG0880042297.01.T01
Chr8 CDS 6688926 6689006 6688926 ID=MsG0880042297.01.T01:cds;Parent=MsG0880042297.01.T01
Chr8 three_prime_UTR 6689007 6689417 6689007 ID=MsG0880042297.01.T01:three_prime_utr;Parent=MsG0880042297.01.T01
Gene Sequence

>MsG0880042297.01.T01

ATACAAAGTCACAAGAAGAAGTTATATGGCATTGATATTGATAGCGTTCTTTCCGCACTAATGTTAACACTCTCACTCTCACTCTCACCCTTTCGTCCTCTTTCTTCCTATTCTTCTCTTTCAATCTCTTCTTCTTCTTCTTCTTCTTTGTCCCAAGTAGTGGCAGTAGCAGAACAAAGCCAACAACAAAAGCCACAATTACGAAGCTCACAACTTGTTGCATTGGAATATGCTGACCTCAATCTCTCTTACAACTTGAATTTGGGTCATGGCAGAATCAGGCAACATGTCAATCCTTCTTCTTTCTCTGTATGCAGCGGCAGGTTTCTTATGTGGCTTGCAAAAAGAACACCTAAAGCGAGAAATCATTTGGGATTGGAAATACGGCAAAAGGTGTTCGTACAATCTGATGCACTTGAAGTGGCACTTGACATGAAAAACCAGTTAGATGAAGTTGATGCACTTAGTCCATAG
Protein sequence

>MsG0880042297.01.T01

IQSHKKKLYGIDIDSVLSALMLTLSLSLSPFRPLSSYSSLSISSSSSSSLSQVVAVAEQSQQQKPQLRSSQLVALEYADLNLSYNLNLGHGRIRQHVNPSSFSVCSGRFLMWLAKRTPKARNHLGLEIRQKVFVQSDALEVALDMKNQLDEVDALSP*