AlfalfaGEDB | MsG0680031826.01

M. sativa cultivar ZhongmuNo.1 / MsG0680031826.01

KEGG: -
GO: -
PFAM: PF01202

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031826.01.T01	XP_024641980.1	90.508	295	25	2	1	293	1	294	0	522
MsG0680031826.01.T02	XP_024641980.1	90.508	295	25	2	1	293	1	294	0	522

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031826.01.T01	Q9LW20	54.444	270	102	4	24	293	32	280	6.12E-92	276
MsG0680031826.01.T02	Q9LW20	54.444	270	102	4	24	293	32	280	6.12E-92	276

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031826.01.T01	A0A072U7L4	90.508	295	25	2	1	293	1	294	0.0	522
MsG0680031826.01.T02	A0A072U7L4	90.508	295	25	2	1	293	1	294	0.0	522

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047885.01	MsG0680031826.01	0.807391	5.172013e-50	2.877247e-47
MsG0080047989.01	MsG0680031826.01	0.816971	4.200431e-52	3.020286e-49
MsG0080048528.01	MsG0680031826.01	0.810390	1.180704e-50	7.107687e-48
MsG0080048727.01	MsG0680031826.01	0.862954	3.513671e-64	1.047584e-60
MsG0080048748.01	MsG0680031826.01	0.869106	3.969610e-66	1.471833e-62
MsG0080048773.01	MsG0680031826.01	0.844174	8.903372e-59	1.425550e-55
MsG0080048780.01	MsG0680031826.01	0.803200	3.905131e-49	1.950438e-46
MsG0080048801.01	MsG0680031826.01	0.811871	5.635865e-51	3.528755e-48
MsG0080048802.01	MsG0680031826.01	0.813639	2.311644e-51	1.517864e-48
MsG0080048820.01	MsG0680031826.01	0.820007	8.609179e-53	6.731345e-50

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031826.01.T01	MTR_6g033510	90.508	295	25	2	1	293	1	294	0.0	522
MsG0680031826.01.T01	MTR_6g033510	90.169	295	25	3	1	293	1	293	0.0	516
MsG0680031826.01.T01	MTR_6g033510	89.492	295	26	3	1	293	1	292	0.0	513
MsG0680031826.01.T01	MTR_6g033510	89.370	254	24	2	1	252	1	253	1.31e-158	443
MsG0680031826.01.T01	MTR_6g033510	92.544	228	17	0	66	293	10	237	8.60e-155	432
MsG0680031826.01.T02	MTR_6g033510	90.508	295	25	2	1	293	1	294	0.0	522
MsG0680031826.01.T02	MTR_6g033510	90.169	295	25	3	1	293	1	293	0.0	516
MsG0680031826.01.T02	MTR_6g033510	89.492	295	26	3	1	293	1	292	0.0	513
MsG0680031826.01.T02	MTR_6g033510	89.370	254	24	2	1	252	1	253	1.31e-158	443
MsG0680031826.01.T02	MTR_6g033510	92.544	228	17	0	66	293	10	237	8.60e-155	432

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680031826.01.T01	AT3G26900	54.444	270	102	4	24	293	32	280	6.24e-93	276
MsG0680031826.01.T01	AT3G26900	54.444	270	102	4	24	293	32	280	6.24e-93	276
MsG0680031826.01.T01	AT3G26900	54.444	270	102	4	24	293	32	280	6.24e-93	276
MsG0680031826.01.T01	AT2G21940	35.354	198	127	1	80	276	89	286	4.20e-40	142
MsG0680031826.01.T01	AT2G21940	35.354	198	127	1	80	276	89	286	4.20e-40	142
MsG0680031826.01.T01	AT2G21940	35.354	198	127	1	80	276	89	286	4.20e-40	142
MsG0680031826.01.T01	AT2G21940	35.354	198	127	1	80	276	90	287	4.88e-40	142
MsG0680031826.01.T01	AT4G39540	35.323	201	127	3	80	277	85	285	2.04e-37	135
MsG0680031826.01.T01	AT4G39540	35.323	201	127	3	80	277	85	285	2.04e-37	135
MsG0680031826.01.T01	AT4G39540	33.482	224	135	5	80	292	85	305	1.88e-34	127

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Find 76 sgRNAs with CRISPR-Local

Find 295 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCAGCAATGACACGGAG+AGG	0.669157	6:+27480972	None:intergenic
AAATTTATTTGGTCAATGGT+TGG	0.508258	6:-27481230	MsG0680031826.01.T01:three_prime_UTR
AACCATTGACCAAATAAATT+TGG	0.160236	6:+27481232	None:intergenic
AACTGAAAGCAGCAATGACA+CGG	0.599211	6:+27480967	None:intergenic
AACTGAAGTATTTGCCGGGT+TGG	0.479658	6:+27481940	None:intergenic
AAGAAAATGGAAAACAATAT+AGG	0.324813	6:+27481347	None:intergenic
AAGAGTAACACCCTCACCAA+TGG	0.626038	6:+27481696	None:intergenic
AAGCAGCAATGACACGGAGA+GGG	0.585712	6:+27480973	None:intergenic
AATGGAAGCATGGCCCAGGT+TGG	0.586297	6:+27480706	None:intergenic
ACAGTTGATGGTTGAAAACA+AGG	0.544648	6:+27480923	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATGATGCTTGTATGGTA+TGG	+	Chr6:27483999-27484018	None:intergenic	30.0%
AAACTATCTTGGATAGCTTA+TGG	+	Chr6:27482667-27482686	None:intergenic	30.0%
AACTAAAAAAGACACATTCG+AGG	+	Chr6:27483247-27483266	None:intergenic	30.0%
AAGCAAAAATGATGCTTGTA+TGG	+	Chr6:27484004-27484023	None:intergenic	30.0%
AAGTGATTTGAAGAAAGAAG+TGG	-	Chr6:27480561-27480580	MsG0680031826.01.T02:three_prime_UTR	30.0%
AATTTGAATGGAACAGAGAT+TGG	+	Chr6:27480729-27480748	None:intergenic	30.0%
ACATGCTCTTAAACTGTTAT+AGG	-	Chr6:27483450-27483469	MsG0680031826.01.T01:intron	30.0%
ACTCTAGTATAGTAACTCTA+AGG	+	Chr6:27481631-27481650	None:intergenic	30.0%
AGAAAGGAAAATGGAAGAAT+TGG	+	Chr6:27484830-27484849	None:intergenic	30.0%
AGTGATTTGAAGAAAGAAGT+GGG	-	Chr6:27480562-27480581	MsG0680031826.01.T02:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	27480527	27485632	27480527	ID=MsG0680031826.01;Name=MsG0680031826.01
Chr6	mRNA	27480576	27485632	27480576	ID=MsG0680031826.01.T01;Parent=MsG0680031826.01;Name=MsG0680031826.01.T01;_AED=0.32;_eAED=0.32;_QI=82\|1\|1\|1\|0.9\|0.81\|11\|1356\|293
Chr6	exon	27480576	27481064	27480576	ID=MsG0680031826.01.T01:exon:8635;Parent=MsG0680031826.01.T01
Chr6	exon	27481096	27482031	27481096	ID=MsG0680031826.01.T01:exon:8634;Parent=MsG0680031826.01.T01
Chr6	five_prime_UTR	27485551	27485632	27485551	ID=MsG0680031826.01.T01:five_prime_utr;Parent=MsG0680031826.01.T01
Chr6	CDS	27485355	27485550	27485355	ID=MsG0680031826.01.T01:cds;Parent=MsG0680031826.01.T01
Chr6	CDS	27484178	27484224	27484178	ID=MsG0680031826.01.T01:cds;Parent=MsG0680031826.01.T01
Chr6	CDS	27483982	27484036	27483982	ID=MsG0680031826.01.T01:cds;Parent=MsG0680031826.01.T01
Chr6	CDS	27483826	27483898	27483826	ID=MsG0680031826.01.T01:cds;Parent=MsG0680031826.01.T01
Chr6	CDS	27483645	27483732	27483645	ID=MsG0680031826.01.T01:cds;Parent=MsG0680031826.01.T01
Chr6	CDS	27483073	27483155	27483073	ID=MsG0680031826.01.T01:cds;Parent=MsG0680031826.01.T01
Chr6	CDS	27482561	27482687	27482561	ID=MsG0680031826.01.T01:cds;Parent=MsG0680031826.01.T01
Chr6	CDS	27482381	27482459	27482381	ID=MsG0680031826.01.T01:cds;Parent=MsG0680031826.01.T01
Chr6	CDS	27482235	27482299	27482235	ID=MsG0680031826.01.T01:cds;Parent=MsG0680031826.01.T01
Chr6	CDS	27481963	27482031	27481963	ID=MsG0680031826.01.T01:cds;Parent=MsG0680031826.01.T01
Chr6	three_prime_UTR	27481096	27481962	27481096	ID=MsG0680031826.01.T01:three_prime_utr;Parent=MsG0680031826.01.T01
Chr6	three_prime_UTR	27480576	27481064	27480576	ID=MsG0680031826.01.T01:three_prime_utr;Parent=MsG0680031826.01.T01
Chr6	mRNA	27480527	27485632	27480527	ID=MsG0680031826.01.T02;Parent=MsG0680031826.01;Name=MsG0680031826.01.T02;_AED=0.40;_eAED=0.40;_QI=82\|1\|1\|1\|0.9\|0.81\|11\|318\|293
Chr6	exon	27480527	27480800	27480527	ID=MsG0680031826.01.T02:exon:8637;Parent=MsG0680031826.01.T02
Chr6	exon	27481919	27482031	27481919	ID=MsG0680031826.01.T02:exon:8636;Parent=MsG0680031826.01.T02
Chr6	five_prime_UTR	27485551	27485632	27485551	ID=MsG0680031826.01.T02:five_prime_utr;Parent=MsG0680031826.01.T02
Chr6	CDS	27485355	27485550	27485355	ID=MsG0680031826.01.T02:cds;Parent=MsG0680031826.01.T02
Chr6	CDS	27484178	27484224	27484178	ID=MsG0680031826.01.T02:cds;Parent=MsG0680031826.01.T02
Chr6	CDS	27483982	27484036	27483982	ID=MsG0680031826.01.T02:cds;Parent=MsG0680031826.01.T02
Chr6	CDS	27483826	27483898	27483826	ID=MsG0680031826.01.T02:cds;Parent=MsG0680031826.01.T02
Chr6	CDS	27483645	27483732	27483645	ID=MsG0680031826.01.T02:cds;Parent=MsG0680031826.01.T02
Chr6	CDS	27483073	27483155	27483073	ID=MsG0680031826.01.T02:cds;Parent=MsG0680031826.01.T02
Chr6	CDS	27482561	27482687	27482561	ID=MsG0680031826.01.T02:cds;Parent=MsG0680031826.01.T02
Chr6	CDS	27482381	27482459	27482381	ID=MsG0680031826.01.T02:cds;Parent=MsG0680031826.01.T02
Chr6	CDS	27482235	27482299	27482235	ID=MsG0680031826.01.T02:cds;Parent=MsG0680031826.01.T02
Chr6	CDS	27481963	27482031	27481963	ID=MsG0680031826.01.T02:cds;Parent=MsG0680031826.01.T02
Chr6	three_prime_UTR	27481919	27481962	27481919	ID=MsG0680031826.01.T02:three_prime_utr;Parent=MsG0680031826.01.T02
Chr6	three_prime_UTR	27480527	27480800	27480527	ID=MsG0680031826.01.T02:three_prime_utr;Parent=MsG0680031826.01.T02

Gene Sequence

>MsG0680031826.01.T01

ATGGAGAAGGCAGCAAGCAGTATGTTGAAGCAGATGAACATCACCAGTCGTCTTGTTCTTCCCGGCAGTTGTTCATCCCTTCAATCTTCATCTTCTTCATCTCTTCACTTTCCATTCCCAATCTCTGTTCCATTCAAATTCCGCAGCAGCAGAAGACCAATTAACTCTGTTTCTGACGCTGCTTTTCCTGTTCCATCTTCAGTGACTAAGGTTTCTGCGACGGATTTATCTCTTGCGGTGAAGAAAAAAGCAGCAGATGTATCCCCTGAGCTGAAAGGGTCCTCCATATTTTTGATTGGTATGAAAAGCTCTCTTAAAACCAATCTGGGAAAACTGCTGGCTGATGTATTGCGATATTATTATTTTGACAGCGATAATTTGGTGGAAGAAGCATTAGGTGGTGCAGCAGCTGCAAAATCCTTCAAAGAGAGTGATGAGACGAGCTTCTATGACTCTGAGACTGAAGTACTTAAGCAATTGTCGTCCATGGGTCGGCTAGTAGTTTGTGCTGGAAATGGCGCTGTACAAAATTCAACTAATCTGGCGCTCCTGAGACATGGGATTACATTCTGGATAGATATGCCTTTAGACATTGTGGCTAGGGATGTAACCGAAAACCAGAGTCAATTTCCTTCATTTGAGATATCTGCTTCAGGATCTTACCCAGAGGTAATGGATGAACTCGGTGCTCTATACAATAAGTACAAAGACGGATATGCTACAGCCGATGCAATTATTTCACTTCAAAAAGTAGCTTCTCAGTTGGGTTATGAAAACGTAGACGACATTACAACAGAAGACATGGCTTTGGAGGTTCTTCGAGAGATTGAGAAGTTGACCAGAGTAAAGAAGATGATGGCAGAAGCTGCAAGACCATTTTAA

>MsG0680031826.01.T02

Protein sequence

>MsG0680031826.01.T01

MEKAASSMLKQMNITSRLVLPGSCSSLQSSSSSSLHFPFPISVPFKFRSSRRPINSVSDAAFPVPSSVTKVSATDLSLAVKKKAADVSPELKGSSIFLIGMKSSLKTNLGKLLADVLRYYYFDSDNLVEEALGGAAAAKSFKESDETSFYDSETEVLKQLSSMGRLVVCAGNGAVQNSTNLALLRHGITFWIDMPLDIVARDVTENQSQFPSFEISASGSYPEVMDELGALYNKYKDGYATADAIISLQKVASQLGYENVDDITTEDMALEVLREIEKLTRVKKMMAEAARPF*

>MsG0680031826.01.T02