AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038056.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038056.01.T01 MTR_7g045270 74.138 116 20 2 1 113 1 109 1.74e-55 173
MsG0780038056.01.T01 MTR_4g036465 31.674 221 109 5 46 236 66 274 7.42e-26 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038056.01.T01 AT4G25170 27.232 224 126 5 47 233 118 341 2.30e-21 91.3
MsG0780038056.01.T01 AT4G25170 27.197 239 122 7 47 233 118 356 3.76e-20 88.2
MsG0780038056.01.T01 AT5G61490 30.846 201 110 7 47 233 70 255 4.96e-17 78.2

Find 36 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTGGAGTTGCCTGATTC+AGG 0.199308 7:+39257129 MsG0780038056.01.T01:CDS
CTCTCTTTGCTCTGAACTCT+TGG 0.224377 7:-39257492 None:intergenic
TAGCCATGATTACAATTAAA+AGG 0.291956 7:-39257697 None:intergenic
TTATCTTCTTGTTGCTGTAA+TGG 0.335551 7:+39255798 MsG0780038056.01.T01:CDS
ATCTTCTGATGAAGCCATTT+GGG 0.337113 7:+39257074 MsG0780038056.01.T01:CDS
TGCAATCTCAAGGATATAAT+AGG 0.358713 7:+39256632 MsG0780038056.01.T01:CDS
TGTGTTGCATCAGAGGCTTC+TGG 0.368361 7:+39257113 MsG0780038056.01.T01:CDS
TATCTTCTGATGAAGCCATT+TGG 0.375555 7:+39257073 MsG0780038056.01.T01:CDS
GCAAGCTGATCCTGCTGTTA+AGG 0.385703 7:+39256678 MsG0780038056.01.T01:CDS
ACTCTTATGATTATGATTCA+AGG 0.402244 7:+39256605 MsG0780038056.01.T01:CDS
GTAAAATACAGACCTGAATC+AGG 0.403971 7:-39257141 None:intergenic
TCTCTTTGCTCTGAACTCTT+GGG 0.413786 7:-39257491 None:intergenic
AACAATCATCATTCATCAAT+GGG 0.416947 7:+39255717 None:intergenic
TCAGCATAGAAATCTTAAAC+TGG 0.423620 7:+39257516 MsG0780038056.01.T01:CDS
AAACAATCATCATTCATCAA+TGG 0.432468 7:+39255716 None:intergenic
TGAGACTGTTCCATCTCAAA+TGG 0.454236 7:+39255878 MsG0780038056.01.T01:CDS
CCTGGGATTGAAAATGCAAC+AGG 0.482841 7:+39257617 MsG0780038056.01.T01:CDS
ATTACAGCAACAAGAAGATA+AGG 0.483282 7:-39255796 None:intergenic
TATCATGAAAGGAAAAGTCT+TGG 0.499321 7:+39257547 MsG0780038056.01.T01:CDS
AATGATTTATTCAAGTACCC+TGG 0.511965 7:+39257599 MsG0780038056.01.T01:CDS
GATGGAACAGTCTCAAATAC+AGG 0.518939 7:-39255870 None:intergenic
TCCTCAACTTCCATTTGAGA+TGG 0.521148 7:-39255888 None:intergenic
TCTTATGACAGCATCCCAAA+TGG 0.524517 7:-39257088 None:intergenic
ATGATTTATTCAAGTACCCT+GGG 0.528335 7:+39257600 MsG0780038056.01.T01:CDS
AGAAAACAAGAGATAACATC+AGG 0.536095 7:+39255831 MsG0780038056.01.T01:CDS
TGGATATTTCATATCATGAA+AGG 0.540393 7:+39257536 MsG0780038056.01.T01:CDS
AAGATAGAAACCTTAACAGC+AGG 0.540636 7:-39256688 None:intergenic
TAAGAAATGTGTTGCATCAG+AGG 0.548569 7:+39257106 MsG0780038056.01.T01:CDS
TCAAGGCTTGTGCAATCTCA+AGG 0.559089 7:+39256622 MsG0780038056.01.T01:CDS
CATCATTCATCAATGGGAGG+TGG 0.560723 7:+39255723 None:intergenic
TACAATTAAAAGGATAACAA+TGG 0.569576 7:-39257687 None:intergenic
TCCATCTCAAATGGAAGTTG+AGG 0.577676 7:+39255887 MsG0780038056.01.T01:CDS
GATCTGTTGATCTATGATTG+TGG 0.615202 7:-39256557 None:intergenic
TTATTGGCGTTACTCAACTG+AGG 0.661229 7:-39255771 None:intergenic
AAACAAGAGATAACATCAGG+TGG 0.661795 7:+39255834 MsG0780038056.01.T01:CDS
AATCATCATTCATCAATGGG+AGG 0.669157 7:+39255720 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAAGAAATATAATTAATTT+AGG + Chr7:39256099-39256118 MsG0780038056.01.T01:intron 10.0%
!!! TGTTATTTCAAAAAATAAAA+GGG + Chr7:39256164-39256183 MsG0780038056.01.T01:intron 10.0%
!!! TTGTTATTTCAAAAAATAAA+AGG + Chr7:39256163-39256182 MsG0780038056.01.T01:intron 10.0%
!! TCGTAAAATGAAATATATAA+TGG + Chr7:39257396-39257415 MsG0780038056.01.T01:intron 15.0%
!!! ACTTTATTAGTGTAATTTTT+TGG + Chr7:39256231-39256250 MsG0780038056.01.T01:intron 15.0%
!!! TGAAATAAATCTGAATTTTT+AGG - Chr7:39256418-39256437 None:intergenic 15.0%
!! AAATATTCACCAAGAATAAA+AGG + Chr7:39257212-39257231 MsG0780038056.01.T01:intron 20.0%
!! AATTTAGTGTAATTATGTGT+TGG + Chr7:39257314-39257333 MsG0780038056.01.T01:intron 20.0%
!! TACAATTAAAAGGATAACAA+TGG - Chr7:39257690-39257709 None:intergenic 20.0%
!!! AAAAAATAAAAGGGACTTTT+GGG + Chr7:39256173-39256192 MsG0780038056.01.T01:intron 20.0%
!!! TATCCTTTTAATTGTAATCA+TGG + Chr7:39257694-39257713 MsG0780038056.01.T01:CDS 20.0%
!!! TGATTTTCTCAAATTATTAG+CGG - Chr7:39257291-39257310 None:intergenic 20.0%
!!! TTAGATGAAATTGTTTGTTT+TGG + Chr7:39256028-39256047 MsG0780038056.01.T01:intron 20.0%
! ACTCTTATGATTATGATTCA+AGG + Chr7:39256605-39256624 MsG0780038056.01.T01:CDS 25.0%
! AGTTTCAAAACACAATCAAT+TGG + Chr7:39256751-39256770 MsG0780038056.01.T01:intron 25.0%
! TAGCCATGATTACAATTAAA+AGG - Chr7:39257700-39257719 None:intergenic 25.0%
! TGGATATTTCATATCATGAA+AGG + Chr7:39257536-39257555 MsG0780038056.01.T01:CDS 25.0%
!! AAAAATAAAAGGGACTTTTG+GGG + Chr7:39256174-39256193 MsG0780038056.01.T01:intron 25.0%
!! ATATTTCATTTTACGAAGCA+TGG - Chr7:39257392-39257411 None:intergenic 25.0%
!! CAAAAAATAAAAGGGACTTT+TGG + Chr7:39256172-39256191 MsG0780038056.01.T01:intron 25.0%
!! CATATCATTCCTTTTATTCT+TGG - Chr7:39257224-39257243 None:intergenic 25.0%
!! TCTGTATTTTACCAGAATTA+TGG + Chr7:39257152-39257171 MsG0780038056.01.T01:intron 25.0%
!! TTCTTGGTGAATATTTCTTT+TGG - Chr7:39257208-39257227 None:intergenic 25.0%
!!! ACAAGAAGATAAGGTTTTAT+TGG - Chr7:39255790-39255809 None:intergenic 25.0%
!!! ACTCTTTTGAGATAAATTAG+AGG + Chr7:39255984-39256003 MsG0780038056.01.T01:intron 25.0%
!!! TTGATTGTGTTTTGAAACTA+TGG - Chr7:39256750-39256769 None:intergenic 25.0%
AAAAGGAATGATATGAAGCA+CGG + Chr7:39257229-39257248 MsG0780038056.01.T01:intron 30.0%
AATGATTTATTCAAGTACCC+TGG + Chr7:39257599-39257618 MsG0780038056.01.T01:CDS 30.0%
AGAAAACAAGAGATAACATC+AGG + Chr7:39255831-39255850 MsG0780038056.01.T01:CDS 30.0%
AGATAAATTAGAGGTCTTCT+TGG + Chr7:39255993-39256012 MsG0780038056.01.T01:intron 30.0%
ATGATTTATTCAAGTACCCT+GGG + Chr7:39257600-39257619 MsG0780038056.01.T01:CDS 30.0%
ATTACAGCAACAAGAAGATA+AGG - Chr7:39255799-39255818 None:intergenic 30.0%
CACAGTTAGATGAAAAAGTT+AGG + Chr7:39257648-39257667 MsG0780038056.01.T01:CDS 30.0%
CATTTCTTCTTCCATAATTC+TGG - Chr7:39257166-39257185 None:intergenic 30.0%
CTTAATACTTAAGCCATGTT+AGG - Chr7:39256923-39256942 None:intergenic 30.0%
GATAAATTAGAGGTCTTCTT+GGG + Chr7:39255994-39256013 MsG0780038056.01.T01:intron 30.0%
TATCATGAAAGGAAAAGTCT+TGG + Chr7:39257547-39257566 MsG0780038056.01.T01:CDS 30.0%
TCAGCATAGAAATCTTAAAC+TGG + Chr7:39257516-39257535 MsG0780038056.01.T01:CDS 30.0%
TGCAATCTCAAGGATATAAT+AGG + Chr7:39256632-39256651 MsG0780038056.01.T01:CDS 30.0%
TGGAAGAAAACAATGTTAGA+TGG - Chr7:39257452-39257471 None:intergenic 30.0%
TTATCTTCTTGTTGCTGTAA+TGG + Chr7:39255798-39255817 MsG0780038056.01.T01:CDS 30.0%
TTGAGTTATATGTCGTACAT+CGG + Chr7:39256785-39256804 MsG0780038056.01.T01:intron 30.0%
! ACTCAATACAAACGATTTTG+AGG - Chr7:39256367-39256386 None:intergenic 30.0%
! GGATTATAACTTGTAGAGAT+TGG + Chr7:39257041-39257060 MsG0780038056.01.T01:intron 30.0%
! GTGTAATTATGTGTTGGTAT+CGG + Chr7:39257320-39257339 MsG0780038056.01.T01:intron 30.0%
! TGTGTTGATGTAATGTTTCT+CGG + Chr7:39257020-39257039 MsG0780038056.01.T01:intron 30.0%
! TTGGTGAATATTTCTTTTGG+TGG - Chr7:39257205-39257224 None:intergenic 30.0%
!! AAAGTGTTGTATGAATCATG+TGG + Chr7:39256454-39256473 MsG0780038056.01.T01:intron 30.0%
!! GAATGCTCAACTTGTTATTA+TGG - Chr7:39256496-39256515 None:intergenic 30.0%
AAACAAGAGATAACATCAGG+TGG + Chr7:39255834-39255853 MsG0780038056.01.T01:CDS 35.0%
AAGATAGAAACCTTAACAGC+AGG - Chr7:39256691-39256710 None:intergenic 35.0%
AATGATATGAAGCACGGATA+CGG + Chr7:39257235-39257254 MsG0780038056.01.T01:intron 35.0%
ATCTTCTGATGAAGCCATTT+GGG + Chr7:39257074-39257093 MsG0780038056.01.T01:CDS 35.0%
CTTTATATTGCTGCGAAATG+CGG + Chr7:39256290-39256309 MsG0780038056.01.T01:intron 35.0%
GATCTGTTGATCTATGATTG+TGG - Chr7:39256560-39256579 None:intergenic 35.0%
GGTTAATGTAGTCGAAATTG+TGG + Chr7:39256321-39256340 MsG0780038056.01.T01:intron 35.0%
GTAAAATACAGACCTGAATC+AGG - Chr7:39257144-39257163 None:intergenic 35.0%
TAAGAAATGTGTTGCATCAG+AGG + Chr7:39257106-39257125 MsG0780038056.01.T01:CDS 35.0%
TAAGAAATGTTACGACCGTT+AGG - Chr7:39256390-39256409 None:intergenic 35.0%
TATCTTCTGATGAAGCCATT+TGG + Chr7:39257073-39257092 MsG0780038056.01.T01:CDS 35.0%
TCGTTTGTATTGAGTCCTAA+CGG + Chr7:39256372-39256391 MsG0780038056.01.T01:intron 35.0%
TCTCAAATTATTAGCGGTGT+CGG - Chr7:39257285-39257304 None:intergenic 35.0%
CGCATTTCGCAGCAATATAA+AGG - Chr7:39256292-39256311 None:intergenic 40.0%
CTGCGAAATGCGGAAATAAA+CGG + Chr7:39256300-39256319 MsG0780038056.01.T01:intron 40.0%
GATGGAACAGTCTCAAATAC+AGG - Chr7:39255873-39255892 None:intergenic 40.0%
TCCATCTCAAATGGAAGTTG+AGG + Chr7:39255887-39255906 MsG0780038056.01.T01:CDS 40.0%
TCCTCAACTTCCATTTGAGA+TGG - Chr7:39255891-39255910 None:intergenic 40.0%
TCTTATGACAGCATCCCAAA+TGG - Chr7:39257091-39257110 None:intergenic 40.0%
TGAGACTGTTCCATCTCAAA+TGG + Chr7:39255878-39255897 MsG0780038056.01.T01:CDS 40.0%
TTATATGTCGTACATCGGAC+AGG + Chr7:39256790-39256809 MsG0780038056.01.T01:intron 40.0%
TTATTGGCGTTACTCAACTG+AGG - Chr7:39255774-39255793 None:intergenic 40.0%
! ACTTGTTTCTCTCACTTGTG+TGG + Chr7:39256852-39256871 MsG0780038056.01.T01:intron 40.0%
! CTGTTGCATTTTCAATCCCA+GGG - Chr7:39257619-39257638 None:intergenic 40.0%
! GATTAAGCGTGTCTTGTTGT+TGG - Chr7:39257359-39257378 None:intergenic 40.0%
! GTCTTTTACCTTTAGCTGGA+TGG - Chr7:39257472-39257491 None:intergenic 40.0%
! GTTTTCTTCCATCCAGCTAA+AGG + Chr7:39257461-39257480 MsG0780038056.01.T01:intron 40.0%
! TCTCTTTGCTCTGAACTCTT+GGG - Chr7:39257494-39257513 None:intergenic 40.0%
! TTATGTGTTGGTATCGGACA+CGG + Chr7:39257326-39257345 MsG0780038056.01.T01:intron 40.0%
! TTGGGTCTTTTACCTTTAGC+TGG - Chr7:39257476-39257495 None:intergenic 40.0%
AACAAGACACGCTTAATCCG+AGG + Chr7:39257361-39257380 MsG0780038056.01.T01:intron 45.0%
CCTGGGATTGAAAATGCAAC+AGG + Chr7:39257617-39257636 MsG0780038056.01.T01:CDS 45.0%
TCAAGGCTTGTGCAATCTCA+AGG + Chr7:39256622-39256641 MsG0780038056.01.T01:CDS 45.0%
! CCTGTTGCATTTTCAATCCC+AGG - Chr7:39257620-39257639 None:intergenic 45.0%
! CTCTCTTTGCTCTGAACTCT+TGG - Chr7:39257495-39257514 None:intergenic 45.0%
AAGCCATGTTAGGTCACGAG+AGG - Chr7:39256913-39256932 None:intergenic 50.0%
ACTCCTCTCGTGACCTAACA+TGG + Chr7:39256907-39256926 MsG0780038056.01.T01:intron 50.0%
GCAAGCTGATCCTGCTGTTA+AGG + Chr7:39256678-39256697 MsG0780038056.01.T01:CDS 50.0%
TACGAAGCATGGACACTCCT+CGG - Chr7:39257381-39257400 None:intergenic 50.0%
TCGTACATCGGACAGGAAAG+TGG + Chr7:39256797-39256816 MsG0780038056.01.T01:intron 50.0%
TGTGTTGCATCAGAGGCTTC+TGG + Chr7:39257113-39257132 MsG0780038056.01.T01:CDS 50.0%
!! CTTCTGGAGTTGCCTGATTC+AGG + Chr7:39257129-39257148 MsG0780038056.01.T01:CDS 50.0%
ACTTGTGTGGTTGCTCTGCC+TGG + Chr7:39256865-39256884 MsG0780038056.01.T01:intron 55.0%
GTTAGGTCACGAGAGGAGTC+CGG - Chr7:39256906-39256925 None:intergenic 55.0%
TGGTTGCTCTGCCTGGATGT+GGG + Chr7:39256872-39256891 MsG0780038056.01.T01:intron 55.0%
TTAGGTCACGAGAGGAGTCC+GGG - Chr7:39256905-39256924 None:intergenic 55.0%
!! GGTGTCGGCATGTCAGTGTT+CGG - Chr7:39257270-39257289 None:intergenic 55.0%
GTGGTTGCTCTGCCTGGATG+TGG + Chr7:39256871-39256890 MsG0780038056.01.T01:intron 60.0%
TAGGTCACGAGAGGAGTCCG+GGG - Chr7:39256904-39256923 None:intergenic 60.0%
TTGCTCTGCCTGGATGTGGG+TGG + Chr7:39256875-39256894 MsG0780038056.01.T01:intron 60.0%
AGGTCACGAGAGGAGTCCGG+GGG - Chr7:39256903-39256922 None:intergenic 65.0%
CTGGATGTGGGTGGTCCCCC+CGG + Chr7:39256884-39256903 MsG0780038056.01.T01:intron 70.0%
GGTCACGAGAGGAGTCCGGG+GGG - Chr7:39256902-39256921 None:intergenic 70.0%
CGGGGGGACCACCCACATCC+AGG - Chr7:39256886-39256905 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr7 gene 39255731 39257803 39255731 ID=MsG0780038056.01;Name=MsG0780038056.01
Chr7 mRNA 39255731 39257803 39255731 ID=MsG0780038056.01.T01;Parent=MsG0780038056.01;Name=MsG0780038056.01.T01;_AED=0.33;_eAED=0.33;_QI=4|1|0.75|1|1|1|4|61|239
Chr7 exon 39257477 39257803 39257477 ID=MsG0780038056.01.T01:exon:9956;Parent=MsG0780038056.01.T01
Chr7 exon 39257057 39257150 39257057 ID=MsG0780038056.01.T01:exon:9955;Parent=MsG0780038056.01.T01
Chr7 exon 39256537 39256699 39256537 ID=MsG0780038056.01.T01:exon:9954;Parent=MsG0780038056.01.T01
Chr7 exon 39255731 39255931 39255731 ID=MsG0780038056.01.T01:exon:9953;Parent=MsG0780038056.01.T01
Chr7 five_prime_UTR 39255731 39255734 39255731 ID=MsG0780038056.01.T01:five_prime_utr;Parent=MsG0780038056.01.T01
Chr7 CDS 39255735 39255931 39255735 ID=MsG0780038056.01.T01:cds;Parent=MsG0780038056.01.T01
Chr7 CDS 39256537 39256699 39256537 ID=MsG0780038056.01.T01:cds;Parent=MsG0780038056.01.T01
Chr7 CDS 39257057 39257150 39257057 ID=MsG0780038056.01.T01:cds;Parent=MsG0780038056.01.T01
Chr7 CDS 39257477 39257742 39257477 ID=MsG0780038056.01.T01:cds;Parent=MsG0780038056.01.T01
Chr7 three_prime_UTR 39257743 39257803 39257743 ID=MsG0780038056.01.T01:three_prime_utr;Parent=MsG0780038056.01.T01
Gene Sequence

>MsG0780038056.01.T01

ATGGGAGGTGGAACTATGCATATTGATCATCTTGTACCTCAGTTGAGTAACGCCAATAAAACCTTATCTTCTTGTTGCTGTAATGGTCAGTTCTGCAGAAAACAAGAGATAACATCAGGTGGTAATCTCTATACTCCTGTATTTGAGACTGTTCCATCTCAAATGGAAGTTGAGGATGCAATTTCAGCTCTTCAAGAATTTATGAAAGCAGTATCAACCACAATCATAGATCAACAGATCGCAGACTTAGATCAACAGATCGCAGACTCTTATGATTATGATTCAAGGCTTGTGCAATCTCAAGGATATAATAGGCTTTACAATGCTCTTCAGTTGCTGCAAGCTGATCCTGCTGTTAAGAGATTGGTGATTTCACTATCTTCTGATGAAGCCATTTGGGATGCTGTCATAAGAAATGTGTTGCATCAGAGGCTTCTGGAGTTGCCTGATTCAGCTAAAGGTAAAAGACCCAAGAGTTCAGAGCAAAGAGAGATCAGCATAGAAATCTTAAACTGGATATTTCATATCATGAAAGGAAAAGTCTTGGAACTGATACAAAGTTTTCAGTCACTTATGAATGATTTATTCAAGTACCCTGGGATTGAAAATGCAACAGGAGATACATCACAGTTAGATGAAAAAGTTAGGTCATCTACACTTCTTTCCATTGTTATCCTTTTAATTGTAATCATGGCTAGATATCAAAGTCGTCAAGTCTAA

Protein sequence

>MsG0780038056.01.T01

MGGGTMHIDHLVPQLSNANKTLSSCCCNGQFCRKQEITSGGNLYTPVFETVPSQMEVEDAISALQEFMKAVSTTIIDQQIADLDQQIADSYDYDSRLVQSQGYNRLYNALQLLQADPAVKRLVISLSSDEAIWDAVIRNVLHQRLLELPDSAKGKRPKSSEQREISIEILNWIFHIMKGKVLELIQSFQSLMNDLFKYPGIENATGDTSQLDEKVRSSTLLSIVILLIVIMARYQSRQV*