Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042732.01.T01 | XP_003627287.1 | 89.151 | 212 | 22 | 1 | 1 | 211 | 1 | 212 | 1.39E-128 | 371 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042732.01.T01 | Q6ZBZ2 | 53.403 | 191 | 85 | 2 | 23 | 211 | 25 | 213 | 1.11E-66 | 207 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042732.01.T01 | G7L6V9 | 89.151 | 212 | 22 | 1 | 1 | 211 | 1 | 212 | 6.63e-129 | 371 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003741.01 | MsG0880042732.01 | 0.812337 | 4.460465e-51 | 2.828481e-48 |
| MsG0180005113.01 | MsG0880042732.01 | 0.810496 | 1.119929e-50 | 6.761093e-48 |
| MsG0180005153.01 | MsG0880042732.01 | 0.812704 | 3.707875e-51 | 2.374116e-48 |
| MsG0880042732.01 | MsG0880045177.01 | 0.818407 | 1.992458e-52 | 1.490267e-49 |
| MsG0380014221.01 | MsG0880042732.01 | 0.806298 | 8.806117e-50 | 4.762611e-47 |
| MsG0680031621.01 | MsG0880042732.01 | 0.814392 | 1.577296e-51 | 1.057102e-48 |
| MsG0480020768.01 | MsG0880042732.01 | 0.814350 | 1.611074e-51 | 1.078445e-48 |
| MsG0680030328.01 | MsG0880042732.01 | 0.805885 | 1.075533e-49 | 5.755939e-47 |
| MsG0280006592.01 | MsG0880042732.01 | 0.834340 | 3.171371e-56 | 3.752704e-53 |
| MsG0280006966.01 | MsG0880042732.01 | 0.807174 | 5.750150e-50 | 3.180848e-47 |
| MsG0280006967.01 | MsG0880042732.01 | 0.805458 | 1.322348e-49 | 6.999547e-47 |
| MsG0280008390.01 | MsG0880042732.01 | 0.810605 | 1.060760e-50 | 6.422709e-48 |
| MsG0280008391.01 | MsG0880042732.01 | 0.805960 | 1.037101e-49 | 5.561046e-47 |
| MsG0280008392.01 | MsG0880042732.01 | 0.818295 | 2.111452e-52 | 1.574250e-49 |
| MsG0280008395.01 | MsG0880042732.01 | 0.801230 | 9.931266e-49 | 4.718066e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042732.01.T01 | MTR_8g020750 | 89.151 | 212 | 22 | 1 | 1 | 211 | 1 | 212 | 1.68e-132 | 371 |
| MsG0880042732.01.T01 | MTR_8g020770 | 66.981 | 212 | 69 | 1 | 1 | 211 | 1 | 212 | 7.68e-101 | 291 |
| MsG0880042732.01.T01 | MTR_4g010340 | 64.762 | 210 | 68 | 4 | 3 | 211 | 1 | 205 | 9.76e-87 | 255 |
| MsG0880042732.01.T01 | MTR_8g020760 | 59.434 | 212 | 84 | 2 | 1 | 211 | 1 | 211 | 1.46e-80 | 239 |
| MsG0880042732.01.T01 | MTR_2g044040 | 55.450 | 211 | 92 | 2 | 1 | 211 | 1 | 209 | 3.02e-78 | 233 |
| MsG0880042732.01.T01 | MTR_2g019780 | 59.259 | 216 | 79 | 5 | 1 | 211 | 1 | 212 | 4.07e-77 | 231 |
| MsG0880042732.01.T01 | MTR_8g020630 | 54.028 | 211 | 95 | 2 | 1 | 211 | 1 | 209 | 6.51e-77 | 230 |
| MsG0880042732.01.T01 | MTR_0284s0010 | 54.976 | 211 | 93 | 2 | 1 | 211 | 1 | 209 | 1.37e-76 | 229 |
| MsG0880042732.01.T01 | MTR_3g019710 | 54.502 | 211 | 94 | 2 | 1 | 211 | 1 | 209 | 1.40e-76 | 229 |
| MsG0880042732.01.T01 | MTR_3g073170 | 57.944 | 214 | 83 | 4 | 1 | 211 | 1 | 210 | 9.46e-76 | 227 |
| MsG0880042732.01.T01 | MTR_2g036120 | 54.286 | 210 | 94 | 2 | 1 | 210 | 1 | 208 | 1.21e-74 | 224 |
| MsG0880042732.01.T01 | MTR_3g019680 | 55.122 | 205 | 90 | 2 | 7 | 211 | 6 | 208 | 7.07e-74 | 222 |
| MsG0880042732.01.T01 | MTR_3g019650 | 54.634 | 205 | 91 | 2 | 7 | 211 | 6 | 208 | 1.26e-73 | 222 |
| MsG0880042732.01.T01 | MTR_8g020650 | 55.851 | 188 | 82 | 1 | 24 | 211 | 23 | 209 | 8.82e-72 | 217 |
| MsG0880042732.01.T01 | MTR_2g045100 | 52.683 | 205 | 96 | 1 | 7 | 211 | 6 | 209 | 7.17e-71 | 214 |
| MsG0880042732.01.T01 | MTR_7g011340 | 56.383 | 188 | 81 | 1 | 24 | 211 | 23 | 209 | 9.12e-71 | 214 |
| MsG0880042732.01.T01 | MTR_8g020763 | 57.692 | 182 | 77 | 0 | 30 | 211 | 9 | 190 | 2.68e-70 | 213 |
| MsG0880042732.01.T01 | MTR_5g031460 | 51.185 | 211 | 101 | 2 | 1 | 211 | 1 | 209 | 2.11e-69 | 211 |
| MsG0880042732.01.T01 | MTR_8g020670 | 50.000 | 208 | 103 | 1 | 4 | 211 | 3 | 209 | 1.31e-67 | 206 |
| MsG0880042732.01.T01 | MTR_2g041550 | 47.642 | 212 | 108 | 2 | 1 | 211 | 1 | 210 | 7.29e-65 | 199 |
| MsG0880042732.01.T01 | MTR_8g020610 | 48.544 | 206 | 103 | 2 | 7 | 211 | 4 | 207 | 1.51e-62 | 193 |
| MsG0880042732.01.T01 | MTR_2g041570 | 44.811 | 212 | 114 | 2 | 1 | 211 | 1 | 210 | 3.56e-62 | 192 |
| MsG0880042732.01.T01 | MTR_8g020590 | 45.238 | 210 | 112 | 2 | 3 | 211 | 1 | 208 | 2.51e-61 | 190 |
| MsG0880042732.01.T01 | MTR_2g041580 | 43.868 | 212 | 116 | 2 | 1 | 211 | 1 | 210 | 3.28e-61 | 190 |
| MsG0880042732.01.T01 | MTR_3g019810 | 43.781 | 201 | 106 | 2 | 4 | 204 | 109 | 302 | 4.01e-50 | 165 |
| MsG0880042732.01.T01 | MTR_6g005330 | 43.128 | 211 | 99 | 6 | 3 | 200 | 1 | 203 | 7.27e-43 | 144 |
| MsG0880042732.01.T01 | MTR_6g005360 | 43.814 | 194 | 91 | 5 | 3 | 183 | 1 | 189 | 3.26e-41 | 139 |
| MsG0880042732.01.T01 | MTR_6g005310 | 44.211 | 190 | 88 | 5 | 7 | 183 | 5 | 189 | 3.75e-41 | 139 |
| MsG0880042732.01.T01 | MTR_6g005340 | 43.684 | 190 | 89 | 5 | 7 | 183 | 5 | 189 | 2.58e-40 | 137 |
| MsG0880042732.01.T01 | MTR_4g027415 | 55.372 | 121 | 53 | 1 | 24 | 144 | 23 | 142 | 3.77e-40 | 134 |
| MsG0880042732.01.T01 | MTR_1g079490 | 39.024 | 205 | 110 | 5 | 14 | 207 | 17 | 217 | 2.03e-37 | 130 |
| MsG0880042732.01.T01 | MTR_1g085140 | 40.415 | 193 | 112 | 2 | 15 | 204 | 20 | 212 | 3.78e-37 | 129 |
| MsG0880042732.01.T01 | MTR_2g030855 | 42.487 | 193 | 96 | 4 | 16 | 202 | 19 | 202 | 4.57e-36 | 126 |
| MsG0880042732.01.T01 | MTR_2g031270 | 43.243 | 185 | 90 | 4 | 24 | 202 | 28 | 203 | 1.65e-35 | 124 |
| MsG0880042732.01.T01 | MTR_1g023770 | 40.704 | 199 | 106 | 3 | 7 | 202 | 10 | 199 | 1.99e-35 | 124 |
| MsG0880042732.01.T01 | MTR_7g111240 | 47.260 | 146 | 76 | 1 | 24 | 168 | 25 | 170 | 5.18e-35 | 123 |
| MsG0880042732.01.T01 | MTR_6g005380 | 43.094 | 181 | 90 | 4 | 3 | 172 | 1 | 179 | 7.10e-35 | 123 |
| MsG0880042732.01.T01 | MTR_1g060120 | 34.158 | 202 | 124 | 3 | 11 | 204 | 18 | 218 | 2.19e-34 | 122 |
| MsG0880042732.01.T01 | MTR_7g111240 | 47.260 | 146 | 73 | 2 | 24 | 168 | 25 | 167 | 4.15e-34 | 121 |
| MsG0880042732.01.T01 | MTR_4g017040 | 38.863 | 211 | 115 | 5 | 1 | 202 | 1 | 206 | 7.50e-34 | 120 |
| MsG0880042732.01.T01 | MTR_5g036570 | 35.789 | 190 | 118 | 3 | 24 | 209 | 32 | 221 | 1.67e-33 | 120 |
| MsG0880042732.01.T01 | MTR_2g019250 | 39.336 | 211 | 111 | 6 | 4 | 202 | 1 | 206 | 3.78e-33 | 119 |
| MsG0880042732.01.T01 | MTR_4g017050 | 38.389 | 211 | 113 | 6 | 4 | 202 | 1 | 206 | 4.03e-33 | 119 |
| MsG0880042732.01.T01 | MTR_5g046410 | 38.389 | 211 | 113 | 6 | 4 | 202 | 1 | 206 | 4.54e-33 | 119 |
| MsG0880042732.01.T01 | MTR_5g046430 | 38.389 | 211 | 113 | 6 | 4 | 202 | 1 | 206 | 4.54e-33 | 119 |
| MsG0880042732.01.T01 | MTR_4g017030 | 38.389 | 211 | 113 | 6 | 4 | 202 | 1 | 206 | 4.54e-33 | 119 |
| MsG0880042732.01.T01 | MTR_1g085500 | 37.255 | 204 | 123 | 3 | 6 | 204 | 8 | 211 | 5.46e-33 | 118 |
| MsG0880042732.01.T01 | MTR_2g072560 | 51.754 | 114 | 54 | 1 | 4 | 117 | 3 | 115 | 7.22e-32 | 113 |
| MsG0880042732.01.T01 | MTR_1g112200 | 33.333 | 210 | 130 | 4 | 4 | 210 | 8 | 210 | 5.86e-31 | 113 |
| MsG0880042732.01.T01 | MTR_2g030895 | 42.424 | 165 | 88 | 3 | 42 | 202 | 7 | 168 | 4.20e-30 | 110 |
| MsG0880042732.01.T01 | MTR_6g005350 | 38.500 | 200 | 105 | 5 | 3 | 189 | 1 | 195 | 2.95e-29 | 108 |
| MsG0880042732.01.T01 | MTR_2g030865 | 42.424 | 165 | 88 | 3 | 42 | 202 | 7 | 168 | 1.39e-28 | 105 |
| MsG0880042732.01.T01 | MTR_8g007300 | 44.030 | 134 | 73 | 2 | 38 | 170 | 47 | 179 | 1.69e-28 | 107 |
| MsG0880042732.01.T01 | MTR_3g019610 | 48.515 | 101 | 51 | 1 | 7 | 107 | 6 | 105 | 1.02e-26 | 99.0 |
| MsG0880042732.01.T01 | MTR_2g031300 | 48.113 | 106 | 52 | 1 | 42 | 144 | 7 | 112 | 1.34e-23 | 92.4 |
| MsG0880042732.01.T01 | MTR_3g096330 | 32.227 | 211 | 130 | 4 | 8 | 206 | 560 | 769 | 8.12e-23 | 96.3 |
| MsG0880042732.01.T01 | MTR_2g086620 | 38.168 | 131 | 79 | 2 | 79 | 207 | 74 | 204 | 9.93e-20 | 83.6 |
| MsG0880042732.01.T01 | MTR_2g086630 | 38.168 | 131 | 79 | 2 | 79 | 207 | 74 | 204 | 1.33e-19 | 83.2 |
| MsG0880042732.01.T01 | MTR_2g096110 | 36.184 | 152 | 70 | 5 | 49 | 196 | 10 | 138 | 7.02e-19 | 79.7 |
| MsG0880042732.01.T01 | MTR_4g052770 | 37.313 | 134 | 82 | 2 | 76 | 207 | 72 | 205 | 1.23e-18 | 80.9 |
| MsG0880042732.01.T01 | MTR_2g086640 | 36.842 | 133 | 80 | 4 | 78 | 207 | 72 | 203 | 1.99e-18 | 80.1 |
| MsG0880042732.01.T01 | MTR_4g052780 | 37.313 | 134 | 82 | 2 | 76 | 207 | 72 | 205 | 2.25e-18 | 80.1 |
| MsG0880042732.01.T01 | MTR_7g029500 | 66.038 | 53 | 18 | 0 | 152 | 204 | 38 | 90 | 4.58e-15 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042732.01.T01 | AT5G20630 | 46.445 | 211 | 111 | 2 | 1 | 210 | 1 | 210 | 1.07e-59 | 186 |
| MsG0880042732.01.T01 | AT1G72610 | 47.090 | 189 | 99 | 1 | 24 | 211 | 20 | 208 | 3.45e-57 | 180 |
| MsG0880042732.01.T01 | AT5G38960 | 45.366 | 205 | 93 | 6 | 20 | 208 | 22 | 223 | 3.45e-46 | 152 |
| MsG0880042732.01.T01 | AT5G38940 | 42.500 | 200 | 102 | 4 | 18 | 207 | 25 | 221 | 6.38e-42 | 141 |
| MsG0880042732.01.T01 | AT5G38940 | 42.500 | 200 | 102 | 4 | 18 | 207 | 49 | 245 | 1.25e-41 | 141 |
| MsG0880042732.01.T01 | AT5G39160 | 40.526 | 190 | 104 | 3 | 25 | 208 | 30 | 216 | 2.41e-41 | 140 |
| MsG0880042732.01.T01 | AT5G39110 | 45.509 | 167 | 85 | 2 | 25 | 185 | 30 | 196 | 2.48e-40 | 137 |
| MsG0880042732.01.T01 | AT3G04200 | 42.051 | 195 | 100 | 4 | 24 | 208 | 32 | 223 | 2.97e-40 | 137 |
| MsG0880042732.01.T01 | AT5G38930 | 40.394 | 203 | 108 | 4 | 15 | 207 | 22 | 221 | 6.74e-40 | 136 |
| MsG0880042732.01.T01 | AT5G39190 | 40.314 | 191 | 107 | 3 | 25 | 208 | 30 | 220 | 9.16e-40 | 136 |
| MsG0880042732.01.T01 | AT5G39120 | 41.624 | 197 | 99 | 4 | 1 | 185 | 3 | 195 | 1.06e-39 | 135 |
| MsG0880042732.01.T01 | AT5G39160 | 40.314 | 191 | 107 | 3 | 25 | 208 | 30 | 220 | 1.12e-39 | 135 |
| MsG0880042732.01.T01 | AT5G39180 | 41.026 | 195 | 101 | 3 | 1 | 185 | 5 | 195 | 1.98e-39 | 135 |
| MsG0880042732.01.T01 | AT5G26700 | 40.394 | 203 | 115 | 3 | 3 | 204 | 9 | 206 | 3.21e-39 | 134 |
| MsG0880042732.01.T01 | AT3G05950 | 46.061 | 165 | 78 | 3 | 25 | 181 | 32 | 193 | 5.10e-39 | 134 |
| MsG0880042732.01.T01 | AT5G39150 | 41.026 | 195 | 101 | 3 | 1 | 185 | 5 | 195 | 6.98e-39 | 134 |
| MsG0880042732.01.T01 | AT4G14630 | 41.538 | 195 | 98 | 4 | 25 | 207 | 32 | 222 | 1.24e-38 | 133 |
| MsG0880042732.01.T01 | AT5G39130 | 39.791 | 191 | 108 | 3 | 25 | 208 | 30 | 220 | 1.87e-38 | 132 |
| MsG0880042732.01.T01 | AT5G38910 | 42.029 | 207 | 101 | 7 | 14 | 207 | 19 | 219 | 1.91e-38 | 132 |
| MsG0880042732.01.T01 | AT1G18980 | 38.537 | 205 | 121 | 3 | 6 | 207 | 16 | 218 | 1.65e-37 | 130 |
| MsG0880042732.01.T01 | AT3G05930 | 44.149 | 188 | 101 | 3 | 24 | 207 | 28 | 215 | 3.67e-37 | 129 |
| MsG0880042732.01.T01 | AT1G09560 | 43.678 | 174 | 94 | 2 | 16 | 185 | 18 | 191 | 7.70e-36 | 125 |
| MsG0880042732.01.T01 | AT1G18970 | 37.915 | 211 | 123 | 4 | 3 | 207 | 10 | 218 | 9.09e-36 | 125 |
| MsG0880042732.01.T01 | AT1G02335 | 37.838 | 185 | 112 | 2 | 24 | 205 | 29 | 213 | 3.43e-35 | 124 |
| MsG0880042732.01.T01 | AT3G04190 | 39.267 | 191 | 109 | 3 | 24 | 207 | 31 | 221 | 5.69e-35 | 124 |
| MsG0880042732.01.T01 | AT5G39190 | 37.037 | 189 | 113 | 2 | 26 | 208 | 10 | 198 | 7.53e-35 | 122 |
| MsG0880042732.01.T01 | AT5G39160 | 37.037 | 189 | 113 | 2 | 26 | 208 | 10 | 198 | 9.14e-35 | 122 |
| MsG0880042732.01.T01 | AT3G04150 | 36.410 | 195 | 117 | 3 | 24 | 211 | 31 | 225 | 3.87e-34 | 122 |
| MsG0880042732.01.T01 | AT5G61750 | 44.118 | 136 | 75 | 1 | 38 | 172 | 40 | 175 | 6.94e-34 | 120 |
| MsG0880042732.01.T01 | AT3G62020 | 36.321 | 212 | 126 | 4 | 5 | 207 | 4 | 215 | 1.50e-33 | 120 |
| MsG0880042732.01.T01 | AT3G04150 | 36.932 | 176 | 105 | 2 | 42 | 211 | 63 | 238 | 1.54e-33 | 120 |
| MsG0880042732.01.T01 | AT3G04180 | 37.895 | 190 | 111 | 3 | 25 | 207 | 32 | 221 | 1.65e-31 | 114 |
| MsG0880042732.01.T01 | AT3G62020 | 37.931 | 174 | 104 | 3 | 38 | 207 | 13 | 186 | 4.21e-30 | 110 |
| MsG0880042732.01.T01 | AT3G04170 | 38.372 | 172 | 97 | 4 | 42 | 207 | 50 | 218 | 4.72e-29 | 108 |
| MsG0880042732.01.T01 | AT1G10460 | 35.928 | 167 | 96 | 4 | 21 | 176 | 16 | 182 | 8.79e-28 | 105 |
| MsG0880042732.01.T01 | AT1G74820 | 39.877 | 163 | 85 | 4 | 4 | 155 | 11 | 171 | 1.77e-27 | 104 |
| MsG0880042732.01.T01 | AT3G10080 | 33.854 | 192 | 116 | 4 | 24 | 207 | 31 | 219 | 2.36e-27 | 104 |
| MsG0880042732.01.T01 | AT3G10080 | 34.375 | 192 | 115 | 4 | 24 | 207 | 102 | 290 | 1.22e-26 | 103 |
| MsG0880042732.01.T01 | AT5G38910 | 33.824 | 204 | 85 | 7 | 14 | 207 | 19 | 182 | 6.94e-21 | 86.3 |
Find 34 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAACTAGTGCTTTGTTATT+TGG | 0.151155 | 8:-13341004 | MsG0880042732.01.T01:CDS |
| TGAAAATGTAATAGTCCTTT+TGG | 0.222682 | 8:+13341094 | None:intergenic |
| GAGGTTAATCCAACTTTAAA+AGG | 0.227849 | 8:+13341325 | None:intergenic |
| AGAATAGATATTGGTATAAA+TGG | 0.231195 | 8:-13341250 | MsG0880042732.01.T01:CDS |
| CTCTTAACCCTGGTGATGTT+TGG | 0.270560 | 8:-13341123 | MsG0880042732.01.T01:CDS |
| TATCACACCTACAAAACTTT+TGG | 0.293680 | 8:-13341152 | MsG0880042732.01.T01:CDS |
| TCTTAACCCTGGTGATGTTT+GGG | 0.343326 | 8:-13341122 | MsG0880042732.01.T01:CDS |
| TTGCTTTCAGACACCCCTTC+AGG | 0.354847 | 8:-13341430 | MsG0880042732.01.T01:CDS |
| CATTGGAGGTAAATGAGATT+AGG | 0.367587 | 8:+13341488 | None:intergenic |
| ATTGGAGGTAAATGAGATTA+GGG | 0.404625 | 8:+13341489 | None:intergenic |
| TGCACAAATTATGAAGCTTA+AGG | 0.415304 | 8:-13340933 | MsG0880042732.01.T01:CDS |
| ATTAGTAATTCAGTTGCATC+AGG | 0.442131 | 8:+13341205 | None:intergenic |
| TACTCTAGCTTAGCAGCTGC+TGG | 0.445392 | 8:-13341361 | MsG0880042732.01.T01:CDS |
| TTTCAAGTTAACTCTGGTGC+TGG | 0.446623 | 8:-13341076 | MsG0880042732.01.T01:CDS |
| GAATTACTAATCGTTGTTCA+AGG | 0.451387 | 8:-13341193 | MsG0880042732.01.T01:CDS |
| AAGGATGTGTTTGGTGGCAG+TGG | 0.462003 | 8:-13340914 | MsG0880042732.01.T01:CDS |
| ATGAAGCTTAAGGATGTGTT+TGG | 0.464532 | 8:-13340923 | MsG0880042732.01.T01:CDS |
| TCTGCAGCAAGAATAGATAT+TGG | 0.476981 | 8:-13341259 | MsG0880042732.01.T01:CDS |
| GCACTAGTTACATGTATGCT+AGG | 0.496669 | 8:+13341016 | None:intergenic |
| AGGTTAATCCAACTTTAAAA+GGG | 0.506034 | 8:+13341326 | None:intergenic |
| AGTAATTTACCAGGTCTCAA+TGG | 0.507923 | 8:-13341292 | MsG0880042732.01.T01:CDS |
| ATCATCAGACTCAATAGCAT+TGG | 0.511181 | 8:+13341471 | None:intergenic |
| ATCAGACTCAATAGCATTGG+AGG | 0.512491 | 8:+13341474 | None:intergenic |
| TGTGTTTGGTGGCAGTGGCT+AGG | 0.536076 | 8:-13340909 | None:intergenic |
| CACTAGTTACATGTATGCTA+GGG | 0.551616 | 8:+13341017 | None:intergenic |
| GATTTGCATGGATAACCTGA+AGG | 0.562918 | 8:+13341415 | None:intergenic |
| AAGCTTAAGGATGTGTTTGG+TGG | 0.598066 | 8:-13340920 | MsG0880042732.01.T01:CDS |
| TTGGTGAAAACTCTTAACCC+TGG | 0.612922 | 8:-13341133 | MsG0880042732.01.T01:CDS |
| GTAAATGTCAGTAATTTACC+AGG | 0.629129 | 8:-13341301 | MsG0880042732.01.T01:CDS |
| AACTTAAGTCCATTGAGACC+TGG | 0.634682 | 8:+13341283 | None:intergenic |
| TAAATTACTGACATTTACTG+AGG | 0.644593 | 8:+13341306 | None:intergenic |
| GCATCAGGATGAGTGTGCAT+TGG | 0.654477 | 8:+13341220 | None:intergenic |
| ATTTGCATGGATAACCTGAA+GGG | 0.657926 | 8:+13341416 | None:intergenic |
| TTTGCATGGATAACCTGAAG+GGG | 0.726823 | 8:+13341417 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAATAGATATTGGTATAAA+TGG | - | Chr8:13341183-13341202 | MsG0880042732.01.T01:CDS | 20.0% |
| !! | CAAAAAAGAAAAGAACAATT+CGG | + | Chr8:13340924-13340943 | None:intergenic | 20.0% |
| ! | TAAATTACTGACATTTACTG+AGG | + | Chr8:13341130-13341149 | None:intergenic | 25.0% |
| !! | AGGTTAATCCAACTTTAAAA+GGG | + | Chr8:13341110-13341129 | None:intergenic | 25.0% |
| !! | TGAAAATGTAATAGTCCTTT+TGG | + | Chr8:13341342-13341361 | None:intergenic | 25.0% |
| !!! | ACAAATTTTTCTGATTTGCA+TGG | + | Chr8:13341033-13341052 | None:intergenic | 25.0% |
| !!! | TTACATTTTCAAGTTAACTC+TGG | - | Chr8:13341351-13341370 | MsG0880042732.01.T01:CDS | 25.0% |
| ATTAGTAATTCAGTTGCATC+AGG | + | Chr8:13341231-13341250 | None:intergenic | 30.0% | |
| ATTGGAGGTAAATGAGATTA+GGG | + | Chr8:13340947-13340966 | None:intergenic | 30.0% | |
| GAATTACTAATCGTTGTTCA+AGG | - | Chr8:13341240-13341259 | MsG0880042732.01.T01:CDS | 30.0% | |
| GAGGTTAATCCAACTTTAAA+AGG | + | Chr8:13341111-13341130 | None:intergenic | 30.0% | |
| GTAAATGTCAGTAATTTACC+AGG | - | Chr8:13341132-13341151 | MsG0880042732.01.T01:CDS | 30.0% | |
| TGCACAAATTATGAAGCTTA+AGG | - | Chr8:13341500-13341519 | MsG0880042732.01.T01:CDS | 30.0% | |
| ! | AAGAGTAGTCTTTTGAATTG+TGG | + | Chr8:13341472-13341491 | None:intergenic | 30.0% |
| ! | TATCACACCTACAAAACTTT+TGG | - | Chr8:13341281-13341300 | MsG0880042732.01.T01:CDS | 30.0% |
| !! | GTAACTAGTGCTTTGTTATT+TGG | - | Chr8:13341429-13341448 | MsG0880042732.01.T01:CDS | 30.0% |
| !! | GTTTTCACCAAAAGTTTTGT+AGG | + | Chr8:13341291-13341310 | None:intergenic | 30.0% |
| !!! | GTTCAAGGTCAATTTTTTGT+TGG | - | Chr8:13341255-13341274 | MsG0880042732.01.T01:CDS | 30.0% |
| AGTAATTTACCAGGTCTCAA+TGG | - | Chr8:13341141-13341160 | MsG0880042732.01.T01:CDS | 35.0% | |
| ATCATCAGACTCAATAGCAT+TGG | + | Chr8:13340965-13340984 | None:intergenic | 35.0% | |
| ATGAAGCTTAAGGATGTGTT+TGG | - | Chr8:13341510-13341529 | MsG0880042732.01.T01:CDS | 35.0% | |
| ATTTGCATGGATAACCTGAA+GGG | + | Chr8:13341020-13341039 | None:intergenic | 35.0% | |
| CACTAGTTACATGTATGCTA+GGG | + | Chr8:13341419-13341438 | None:intergenic | 35.0% | |
| CATTGGAGGTAAATGAGATT+AGG | + | Chr8:13340948-13340967 | None:intergenic | 35.0% | |
| TCTGCAGCAAGAATAGATAT+TGG | - | Chr8:13341174-13341193 | MsG0880042732.01.T01:CDS | 35.0% | |
| ! | GTCTTTTGAATTGTGGAAGT+TGG | + | Chr8:13341465-13341484 | None:intergenic | 35.0% |
| !! | GATGTTTGGGTTTTTCCAAA+AGG | - | Chr8:13341324-13341343 | MsG0880042732.01.T01:CDS | 35.0% |
| !!! | ACAGCAAACCCTTTTAAAGT+TGG | - | Chr8:13341099-13341118 | MsG0880042732.01.T01:CDS | 35.0% |
| AACTTAAGTCCATTGAGACC+TGG | + | Chr8:13341153-13341172 | None:intergenic | 40.0% | |
| AAGCTTAAGGATGTGTTTGG+TGG | - | Chr8:13341513-13341532 | MsG0880042732.01.T01:CDS | 40.0% | |
| GAAAAACCCAAACATCACCA+GGG | + | Chr8:13341320-13341339 | None:intergenic | 40.0% | |
| GATTTGCATGGATAACCTGA+AGG | + | Chr8:13341021-13341040 | None:intergenic | 40.0% | |
| GCACTAGTTACATGTATGCT+AGG | + | Chr8:13341420-13341439 | None:intergenic | 40.0% | |
| TTGGTGAAAACTCTTAACCC+TGG | - | Chr8:13341300-13341319 | MsG0880042732.01.T01:CDS | 40.0% | |
| TTTGCATGGATAACCTGAAG+GGG | + | Chr8:13341019-13341038 | None:intergenic | 40.0% | |
| ! | ATCAGACTCAATAGCATTGG+AGG | + | Chr8:13340962-13340981 | None:intergenic | 40.0% |
| ! | TCTTAACCCTGGTGATGTTT+GGG | - | Chr8:13341311-13341330 | MsG0880042732.01.T01:CDS | 40.0% |
| !! | TTTCAAGTTAACTCTGGTGC+TGG | - | Chr8:13341357-13341376 | MsG0880042732.01.T01:CDS | 40.0% |
| GGAAAAACCCAAACATCACC+AGG | + | Chr8:13341321-13341340 | None:intergenic | 45.0% | |
| ! | CTCTTAACCCTGGTGATGTT+TGG | - | Chr8:13341310-13341329 | MsG0880042732.01.T01:CDS | 45.0% |
| GCATCAGGATGAGTGTGCAT+TGG | + | Chr8:13341216-13341235 | None:intergenic | 50.0% | |
| TTGCTTTCAGACACCCCTTC+AGG | - | Chr8:13341003-13341022 | MsG0880042732.01.T01:CDS | 50.0% | |
| ! | AAGGATGTGTTTGGTGGCAG+TGG | - | Chr8:13341519-13341538 | MsG0880042732.01.T01:CDS | 50.0% |
| ! | TACTCTAGCTTAGCAGCTGC+TGG | - | Chr8:13341072-13341091 | MsG0880042732.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 13340910 | 13341545 | 13340910 | ID=MsG0880042732.01;Name=MsG0880042732.01 |
| Chr8 | mRNA | 13340910 | 13341545 | 13340910 | ID=MsG0880042732.01.T01;Parent=MsG0880042732.01;Name=MsG0880042732.01.T01;_AED=0.16;_eAED=0.16;_QI=0|-1|0|1|-1|1|1|0|211 |
| Chr8 | exon | 13340910 | 13341545 | 13340910 | ID=MsG0880042732.01.T01:exon:36800;Parent=MsG0880042732.01.T01 |
| Chr8 | CDS | 13340910 | 13341545 | 13340910 | ID=MsG0880042732.01.T01:cds;Parent=MsG0880042732.01.T01 |
| Gene Sequence |
| Protein sequence |
