AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044027.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044027.01.T01 MTR_8g445140 79.605 152 31 0 23 174 152 303 8.60e-88 260
MsG0880044027.01.T01 MTR_8g445170 70.690 174 49 1 16 187 224 397 2.45e-87 262
MsG0880044027.01.T01 MTR_8g045890 72.671 161 44 0 27 187 285 445 1.46e-86 261
MsG0880044027.01.T01 MTR_8g045890 72.671 161 44 0 27 187 326 486 5.37e-86 261
MsG0880044027.01.T01 MTR_8g045890 72.671 161 44 0 27 187 363 523 1.35e-85 261
MsG0880044027.01.T01 MTR_8g045880 76.250 160 38 0 28 187 169 328 5.39e-84 251
MsG0880044027.01.T01 MTR_8g045880 76.250 160 38 0 28 187 204 363 1.32e-83 251
MsG0880044027.01.T01 MTR_8g045880 76.250 160 38 0 28 187 206 365 1.72e-83 251
MsG0880044027.01.T01 MTR_8g046000 62.500 176 66 0 12 187 61 236 3.56e-82 243
MsG0880044027.01.T01 MTR_1g045860 62.570 179 59 3 13 187 95 269 5.62e-77 231
MsG0880044027.01.T01 MTR_8g044290 73.485 132 33 1 18 147 209 340 3.16e-65 207
MsG0880044027.01.T01 MTR_2g081610 49.091 165 75 2 19 174 96 260 5.59e-58 183
MsG0880044027.01.T01 MTR_2g081770 48.913 184 85 4 1 176 91 273 1.13e-56 180
MsG0880044027.01.T01 MTR_2g081590 47.904 167 78 2 17 174 94 260 6.11e-53 170
MsG0880044027.01.T01 MTR_3g109490 46.622 148 69 3 36 173 174 321 3.22e-40 139
MsG0880044027.01.T01 MTR_1g008470 46.099 141 71 4 36 171 157 297 1.09e-38 134
MsG0880044027.01.T01 MTR_0312s0030 44.828 145 74 4 34 172 179 323 1.33e-37 132
MsG0880044027.01.T01 MTR_1g007520 44.828 145 74 4 34 172 179 323 1.33e-37 132
MsG0880044027.01.T01 MTR_1g008590 44.828 145 74 4 34 172 179 323 1.77e-37 132
MsG0880044027.01.T01 MTR_1g008530 43.836 146 75 4 34 172 77 222 2.51e-37 129
MsG0880044027.01.T01 MTR_1g008580 43.243 148 77 4 34 174 25 172 2.91e-37 127
MsG0880044027.01.T01 MTR_1g008460 45.775 142 72 4 35 171 153 294 3.17e-37 130
MsG0880044027.01.T01 MTR_1g008500 42.466 146 77 3 34 172 238 383 5.89e-36 129
MsG0880044027.01.T01 MTR_1g008560 43.836 146 75 4 34 172 183 328 6.50e-36 128
MsG0880044027.01.T01 MTR_1g008510 43.836 146 75 4 34 172 179 324 1.10e-35 127
MsG0880044027.01.T01 MTR_1g008450 44.030 134 70 4 43 171 177 310 3.34e-31 115
MsG0880044027.01.T01 MTR_3g116380 39.286 140 76 5 43 173 163 302 5.54e-23 93.6
MsG0880044027.01.T01 MTR_3g107830 36.486 148 84 4 36 173 108 255 2.11e-21 88.6
MsG0880044027.01.T01 MTR_4g069680 37.162 148 83 5 36 173 139 286 6.01e-21 87.8
MsG0880044027.01.T01 MTR_3g116410 36.913 149 84 5 36 174 128 276 4.05e-20 85.5
MsG0880044027.01.T01 MTR_3g116320 36.879 141 79 5 43 173 547 687 1.39e-18 83.2
MsG0880044027.01.T01 MTR_4g069570 34.000 150 87 7 36 173 186 335 3.10e-18 80.9
MsG0880044027.01.T01 MTR_3g116270 36.879 141 79 5 43 173 365 505 9.49e-18 80.5
MsG0880044027.01.T01 MTR_4g069550 34.000 150 87 6 36 173 313 462 4.50e-17 78.6
MsG0880044027.01.T01 MTR_4g069040 32.432 148 88 7 43 178 367 514 6.84e-17 78.2
MsG0880044027.01.T01 MTR_8g064500 33.784 148 88 5 34 172 225 371 1.52e-15 73.9
MsG0880044027.01.T01 MTR_8g064560 33.103 145 87 5 36 171 480 623 3.94e-15 73.2
MsG0880044027.01.T01 MTR_5g034320 30.556 144 92 4 36 171 483 626 6.13e-14 69.7
MsG0880044027.01.T01 MTR_8g064530 31.507 146 92 4 34 171 495 640 7.98e-14 69.3
MsG0880044027.01.T01 MTR_4g133840 29.655 145 93 4 36 171 42 186 1.77e-13 65.9
MsG0880044027.01.T01 MTR_4g068420 31.061 132 72 6 48 173 2 120 3.90e-12 60.8
MsG0880044027.01.T01 MTR_6g086530 29.861 144 89 6 43 174 63 206 2.93e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044027.01.T01 AT5G25610 36.788 193 97 7 4 171 197 389 3.43e-34 125
MsG0880044027.01.T01 AT1G49320 35.714 154 84 7 34 172 125 278 1.50e-20 86.7
MsG0880044027.01.T01 AT1G70370 31.250 144 91 4 36 171 479 622 2.92e-17 79.3
MsG0880044027.01.T01 AT1G70370 31.250 144 91 4 36 171 479 622 2.92e-17 79.3
MsG0880044027.01.T01 AT1G60390 31.544 149 94 5 31 171 472 620 3.57e-15 73.2
MsG0880044027.01.T01 AT1G23760 30.345 145 91 5 36 171 475 618 9.74e-15 72.0

Find 47 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATATATTGGTCTTGGTTT+TGG 0.131953 8:+34541261 None:intergenic
GTAGAGGAAGTGAAGAAAAT+TGG 0.216702 8:-34541239 MsG0880044027.01.T01:CDS
CGAAATCTATCATTGATTCT+AGG 0.249913 8:+34541333 None:intergenic
AAAGCTTGGAACAAATATTA+AGG 0.268182 8:-34541303 MsG0880044027.01.T01:CDS
GTGTGAACCCAGCAGCTATA+GGG 0.326521 8:-34541379 MsG0880044027.01.T01:CDS
TTGTGTTCCCCTATAGCTGC+TGG 0.326965 8:+34541371 None:intergenic
TGGAAGAATAAGACAACCTT+TGG 0.342882 8:-34541444 MsG0880044027.01.T01:CDS
TCACTTCCTCTACTATATAT+TGG 0.350269 8:+34541249 None:intergenic
TGACATATTGGAATTGTTCC+AGG 0.361803 8:+34541023 None:intergenic
CCTCTACTATATATTGGTCT+TGG 0.371712 8:+34541255 None:intergenic
TTGCTAACAAAATGACATAT+TGG 0.384980 8:+34541011 None:intergenic
ATTTGTTCCAAGCTTTGAAA+TGG 0.389440 8:+34541310 None:intergenic
GATTTCGCCATTTCAAAGCT+TGG 0.391238 8:-34541317 MsG0880044027.01.T01:CDS
AACCTTTGGGAACATGGATA+TGG 0.395939 8:-34541430 MsG0880044027.01.T01:CDS
AACATACATAGTTCCTTTAG+TGG 0.396577 8:-34541141 MsG0880044027.01.T01:CDS
AGACTCTCTTACAACATGAT+TGG 0.417528 8:+34540968 None:intergenic
GGTTGTCTTATTCTTCCAAC+AGG 0.420121 8:+34541449 None:intergenic
GCTTCCACAAGCATTCTGAT+AGG 0.446365 8:-34541475 MsG0880044027.01.T01:CDS
TGTGTGAACCCAGCAGCTAT+AGG 0.462266 8:-34541380 MsG0880044027.01.T01:CDS
AGAACTTCATAAAGCACGTC+AGG 0.473905 8:+34541056 None:intergenic
GACTCTCTTACAACATGATT+GGG 0.475130 8:+34540969 None:intergenic
GTTCCATCAGAGGCCACTAA+AGG 0.489886 8:+34541128 None:intergenic
GAAATCTATCATTGATTCTA+GGG 0.494556 8:+34541334 None:intergenic
TGTGTTCCCCTATAGCTGCT+GGG 0.500356 8:+34541372 None:intergenic
GTTCCTTTAGTGGCCTCTGA+TGG 0.500787 8:-34541131 MsG0880044027.01.T01:CDS
TTGATGTTGCATTGACTTGG+TGG 0.512892 8:+34541162 None:intergenic
GAAGTTCTCAAAGTCAAGCC+TGG 0.515439 8:-34541041 MsG0880044027.01.T01:CDS
CCTGGAAAGAAAATGAGCTT+AGG 0.515909 8:-34541497 None:intergenic
TAAGACAACCTTTGGGAACA+TGG 0.519820 8:-34541436 MsG0880044027.01.T01:CDS
CCACCATGACACGAGAGGTA+TGG 0.526766 8:-34541081 MsG0880044027.01.T01:CDS
GTATCCTATCAGAATGCTTG+TGG 0.540753 8:+34541471 None:intergenic
ATGTTGATGTTGCATTGACT+TGG 0.542701 8:+34541159 None:intergenic
GGAAGAATAAGACAACCTTT+GGG 0.545608 8:-34541443 MsG0880044027.01.T01:CDS
CTTCGCTTTAGTTCCATCAG+AGG 0.557690 8:+34541118 None:intergenic
TGTGAACCCAGCAGCTATAG+GGG 0.559123 8:-34541378 MsG0880044027.01.T01:CDS
CATTCTGATAGGATACCTGT+TGG 0.559391 8:-34541464 MsG0880044027.01.T01:CDS
ATTTAGTTTATGACACATCA+CGG 0.560330 8:+34541208 None:intergenic
GATCCATACCTCTCGTGTCA+TGG 0.564641 8:+34541078 None:intergenic
CTCCATATCCATGTTCCCAA+AGG 0.572259 8:+34541428 None:intergenic
CTCTGATGGAACTAAAGCGA+AGG 0.574492 8:-34541117 MsG0880044027.01.T01:CDS
TAGCAACGAGGCCATTGCAT+GGG 0.577381 8:-34540994 MsG0880044027.01.T01:CDS
TTAGCAACGAGGCCATTGCA+TGG 0.601905 8:-34540995 MsG0880044027.01.T01:CDS
ATGATTGGGTACCCATGCAA+TGG 0.623581 8:+34540983 None:intergenic
CCAAGACCAATATATAGTAG+AGG 0.626479 8:-34541255 MsG0880044027.01.T01:CDS
CCATACCTCTCGTGTCATGG+TGG 0.655956 8:+34541081 None:intergenic
GACACAGATTATATGACACA+AGG 0.694555 8:+34540936 None:intergenic
ATATGCCACCATGACACGAG+AGG 0.699219 8:-34541086 MsG0880044027.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGCTTGGAACAAATATTA+AGG - Chr8:34541126-34541145 MsG0880044027.01.T01:CDS 25.0%
! TTGCTAACAAAATGACATAT+TGG + Chr8:34541421-34541440 None:intergenic 25.0%
!! ATTTAGTTTATGACACATCA+CGG + Chr8:34541224-34541243 None:intergenic 25.0%
!!! GAAATCTATCATTGATTCTA+GGG + Chr8:34541098-34541117 None:intergenic 25.0%
!!! TATATATTGGTCTTGGTTTT+GGG + Chr8:34541170-34541189 None:intergenic 25.0%
AACATACATAGTTCCTTTAG+TGG - Chr8:34541288-34541307 MsG0880044027.01.T01:CDS 30.0%
TCACTTCCTCTACTATATAT+TGG + Chr8:34541183-34541202 None:intergenic 30.0%
!! ATTTGTTCCAAGCTTTGAAA+TGG + Chr8:34541122-34541141 None:intergenic 30.0%
!! CGAAATCTATCATTGATTCT+AGG + Chr8:34541099-34541118 None:intergenic 30.0%
!!! CTATATATTGGTCTTGGTTT+TGG + Chr8:34541171-34541190 None:intergenic 30.0%
AGACTCTCTTACAACATGAT+TGG + Chr8:34541464-34541483 None:intergenic 35.0%
CCAAGACCAATATATAGTAG+AGG - Chr8:34541174-34541193 MsG0880044027.01.T01:CDS 35.0%
GACTCTCTTACAACATGATT+GGG + Chr8:34541463-34541482 None:intergenic 35.0%
GGAAGAATAAGACAACCTTT+GGG - Chr8:34540986-34541005 MsG0880044027.01.T01:CDS 35.0%
GTAGAGGAAGTGAAGAAAAT+TGG - Chr8:34541190-34541209 MsG0880044027.01.T01:CDS 35.0%
TGACATATTGGAATTGTTCC+AGG + Chr8:34541409-34541428 None:intergenic 35.0%
TGGAAGAATAAGACAACCTT+TGG - Chr8:34540985-34541004 MsG0880044027.01.T01:CDS 35.0%
! CCTCTACTATATATTGGTCT+TGG + Chr8:34541177-34541196 None:intergenic 35.0%
!! ATGTTGATGTTGCATTGACT+TGG + Chr8:34541273-34541292 None:intergenic 35.0%
!!! ATGTCATTTTGTTAGCAACG+AGG - Chr8:34541423-34541442 MsG0880044027.01.T01:CDS 35.0%
AGAACTTCATAAAGCACGTC+AGG + Chr8:34541376-34541395 None:intergenic 40.0%
CATTCTGATAGGATACCTGT+TGG - Chr8:34540965-34540984 MsG0880044027.01.T01:CDS 40.0%
GATTTCGCCATTTCAAAGCT+TGG - Chr8:34541112-34541131 MsG0880044027.01.T01:CDS 40.0%
GTATCCTATCAGAATGCTTG+TGG + Chr8:34540961-34540980 None:intergenic 40.0%
! AACCTTTGGGAACATGGATA+TGG - Chr8:34540999-34541018 MsG0880044027.01.T01:CDS 40.0%
! GGTTGTCTTATTCTTCCAAC+AGG + Chr8:34540983-34541002 None:intergenic 40.0%
! TAAGACAACCTTTGGGAACA+TGG - Chr8:34540993-34541012 MsG0880044027.01.T01:CDS 40.0%
!! TTGATGTTGCATTGACTTGG+TGG + Chr8:34541270-34541289 None:intergenic 40.0%
ATGATTGGGTACCCATGCAA+TGG + Chr8:34541449-34541468 None:intergenic 45.0%
CTCCATATCCATGTTCCCAA+AGG + Chr8:34541004-34541023 None:intergenic 45.0%
CTCTGATGGAACTAAAGCGA+AGG - Chr8:34541312-34541331 MsG0880044027.01.T01:CDS 45.0%
GAAGTTCTCAAAGTCAAGCC+TGG - Chr8:34541388-34541407 MsG0880044027.01.T01:CDS 45.0%
GCTTCCACAAGCATTCTGAT+AGG - Chr8:34540954-34540973 MsG0880044027.01.T01:CDS 45.0%
! CTTCGCTTTAGTTCCATCAG+AGG + Chr8:34541314-34541333 None:intergenic 45.0%
!!! TTGGTCTTGGTTTTGGGCAA+AGG + Chr8:34541164-34541183 None:intergenic 45.0%
ATATGCCACCATGACACGAG+AGG - Chr8:34541343-34541362 MsG0880044027.01.T01:CDS 50.0%
GATCCATACCTCTCGTGTCA+TGG + Chr8:34541354-34541373 None:intergenic 50.0%
GTGTGAACCCAGCAGCTATA+GGG - Chr8:34541050-34541069 MsG0880044027.01.T01:CDS 50.0%
GTTCCATCAGAGGCCACTAA+AGG + Chr8:34541304-34541323 None:intergenic 50.0%
GTTCCTTTAGTGGCCTCTGA+TGG - Chr8:34541298-34541317 MsG0880044027.01.T01:CDS 50.0%
TAGCAACGAGGCCATTGCAT+GGG - Chr8:34541435-34541454 MsG0880044027.01.T01:CDS 50.0%
TGTGAACCCAGCAGCTATAG+GGG - Chr8:34541051-34541070 MsG0880044027.01.T01:CDS 50.0%
TGTGTGAACCCAGCAGCTAT+AGG - Chr8:34541049-34541068 MsG0880044027.01.T01:CDS 50.0%
TGTGTTCCCCTATAGCTGCT+GGG + Chr8:34541060-34541079 None:intergenic 50.0%
TTAGCAACGAGGCCATTGCA+TGG - Chr8:34541434-34541453 MsG0880044027.01.T01:CDS 50.0%
TTGTGTTCCCCTATAGCTGC+TGG + Chr8:34541061-34541080 None:intergenic 50.0%
CCACCATGACACGAGAGGTA+TGG - Chr8:34541348-34541367 MsG0880044027.01.T01:CDS 55.0%
CCATACCTCTCGTGTCATGG+TGG + Chr8:34541351-34541370 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 34540944 34541507 34540944 ID=MsG0880044027.01;Name=MsG0880044027.01
Chr8 mRNA 34540944 34541507 34540944 ID=MsG0880044027.01.T01;Parent=MsG0880044027.01;Name=MsG0880044027.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|187
Chr8 exon 34540944 34541507 34540944 ID=MsG0880044027.01.T01:exon:15969;Parent=MsG0880044027.01.T01
Chr8 CDS 34540944 34541507 34540944 ID=MsG0880044027.01.T01:cds;Parent=MsG0880044027.01.T01
Gene Sequence

>MsG0880044027.01.T01

ATGAGCTTAGGCTTCCACAAGCATTCTGATAGGATACCTGTTGGAAGAATAAGACAACCTTTGGGAACATGGATATGGAGTGAAACACATAGTCTTTATAGCTATTGTGTGAACCCAGCAGCTATAGGGGAACACAAGTATTGCGCATCATCCCTAGAATCAATGATAGATTTCGCCATTTCAAAGCTTGGAACAAATATTAAGGTGATTTCAAGTTCCTTTGCCCAAAACCAAGACCAATATATAGTAGAGGAAGTGAAGAAAATTGGAGACAAAGCCGTGATGTGTCATAAACTAAATTTCGAAGAAGTTGTATTTTATTGCCACCAAGTCAATGCAACATCAACATACATAGTTCCTTTAGTGGCCTCTGATGGAACTAAAGCGAAGGCACTAACAATATGCCACCATGACACGAGAGGTATGGATCCTGACGTGCTTTATGAAGTTCTCAAAGTCAAGCCTGGAACAATTCCAATATGTCATTTTGTTAGCAACGAGGCCATTGCATGGGTACCCAATCATGTTGTAAGAGAGTCTGATGAGAATCCTTGTGTCATATAA

Protein sequence

>MsG0880044027.01.T01

MSLGFHKHSDRIPVGRIRQPLGTWIWSETHSLYSYCVNPAAIGEHKYCASSLESMIDFAISKLGTNIKVISSSFAQNQDQYIVEEVKKIGDKAVMCHKLNFEEVVFYCHQVNATSTYIVPLVASDGTKAKALTICHHDTRGMDPDVLYEVLKVKPGTIPICHFVSNEAIAWVPNHVVRESDENPCVI*