Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043998.01.T01 | XP_024627598.1 | 83.394 | 277 | 15 | 2 | 1 | 249 | 1 | 274 | 5.00E-166 | 469 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043998.01.T01 | Q43675 | 55.652 | 230 | 88 | 5 | 24 | 249 | 49 | 268 | 1.01E-82 | 251 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043998.01.T01 | A0A396GLU8 | 83.394 | 277 | 15 | 2 | 1 | 249 | 1 | 274 | 2.39e-166 | 469 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049008.01 | MsG0880043998.01 | 0.801077 | 1.067449e-48 | 5.051768e-46 |
| MsG0180001029.01 | MsG0880043998.01 | 0.819884 | 9.187188e-53 | 7.158415e-50 |
| MsG0180001499.01 | MsG0880043998.01 | 0.814941 | 1.192337e-51 | 8.109420e-49 |
| MsG0880043998.01 | MsG0880044027.01 | 0.952772 | 1.176560e-110 | 2.943900e-105 |
| MsG0880043998.01 | MsG0880046402.01 | 0.802960 | 4.378118e-49 | 2.173289e-46 |
| MsG0880043998.01 | MsG0880046870.01 | 0.811390 | 7.172251e-51 | 4.433920e-48 |
| MsG0880043998.01 | MsG0880047424.01 | 0.808741 | 2.668769e-50 | 1.537961e-47 |
| MsG0880043998.01 | MsG0880047714.01 | 0.813604 | 2.353217e-51 | 1.543712e-48 |
| MsG0180003320.01 | MsG0880043998.01 | 0.806436 | 8.234190e-50 | 4.469035e-47 |
| MsG0180003375.01 | MsG0880043998.01 | 0.810576 | 1.076315e-50 | 6.511721e-48 |
| MsG0180003484.01 | MsG0880043998.01 | 0.819600 | 1.066663e-52 | 8.245299e-50 |
| MsG0180004549.01 | MsG0880043998.01 | 0.816632 | 5.003752e-52 | 3.564392e-49 |
| MsG0180004610.01 | MsG0880043998.01 | 0.807851 | 4.129692e-50 | 2.324980e-47 |
| MsG0780040783.01 | MsG0880043998.01 | 0.815649 | 8.298000e-52 | 5.754544e-49 |
| MsG0780041397.01 | MsG0880043998.01 | 0.815503 | 8.943036e-52 | 6.177041e-49 |
| MsG0880042591.01 | MsG0880043998.01 | 0.834812 | 2.413883e-56 | 2.896639e-53 |
| MsG0880043550.01 | MsG0880043998.01 | 0.826586 | 2.501601e-54 | 2.355778e-51 |
| MsG0880043607.01 | MsG0880043998.01 | 0.807324 | 5.344032e-50 | 2.967792e-47 |
| MsG0880043669.01 | MsG0880043998.01 | 0.808468 | 3.051551e-50 | 1.746240e-47 |
| MsG0880043882.01 | MsG0880043998.01 | 0.803012 | 4.269962e-49 | 2.122326e-46 |
| MsG0380011686.01 | MsG0880043998.01 | 0.833716 | 4.545859e-56 | 5.278503e-53 |
| MsG0380011701.01 | MsG0880043998.01 | 0.802968 | 4.361909e-49 | 2.165633e-46 |
| MsG0380012889.01 | MsG0880043998.01 | 0.801930 | 7.137449e-49 | 3.451256e-46 |
| MsG0380012900.01 | MsG0880043998.01 | 0.817486 | 3.216160e-52 | 2.345343e-49 |
| MsG0380013843.01 | MsG0880043998.01 | 0.830874 | 2.296060e-55 | 2.450355e-52 |
| MsG0480019663.01 | MsG0880043998.01 | 0.837970 | 3.795286e-57 | 5.011722e-54 |
| MsG0480020810.01 | MsG0880043998.01 | 0.816094 | 6.601601e-52 | 4.634134e-49 |
| MsG0480020904.01 | MsG0880043998.01 | 0.800917 | 1.150938e-48 | 5.424809e-46 |
| MsG0480021227.01 | MsG0880043998.01 | 0.835818 | 1.344722e-56 | 1.663174e-53 |
| MsG0480022310.01 | MsG0880043998.01 | 0.817194 | 3.742085e-52 | 2.706933e-49 |
| MsG0480022428.01 | MsG0880043998.01 | 0.827813 | 1.271170e-54 | 1.240590e-51 |
| MsG0480022833.01 | MsG0880043998.01 | 0.808168 | 3.535704e-50 | 2.007440e-47 |
| MsG0580024176.01 | MsG0880043998.01 | 0.813876 | 2.049885e-51 | 1.354697e-48 |
| MsG0580024207.01 | MsG0880043998.01 | 0.819062 | 1.414521e-52 | 1.077238e-49 |
| MsG0580024220.01 | MsG0880043998.01 | 0.812480 | 4.151247e-51 | 2.642476e-48 |
| MsG0580024412.01 | MsG0880043998.01 | 0.813019 | 3.162820e-51 | 2.042061e-48 |
| MsG0580024980.01 | MsG0880043998.01 | 0.801194 | 1.010333e-48 | 4.795392e-46 |
| MsG0580025023.01 | MsG0880043998.01 | 0.819284 | 1.258850e-52 | 9.645464e-50 |
| MsG0380016372.01 | MsG0880043998.01 | 0.800300 | 1.537838e-48 | 7.135814e-46 |
| MsG0380016717.01 | MsG0880043998.01 | 0.813491 | 2.491451e-51 | 1.629477e-48 |
| MsG0380017901.01 | MsG0880043998.01 | 0.824579 | 7.476370e-54 | 6.649888e-51 |
| MsG0380018004.01 | MsG0880043998.01 | 0.801589 | 8.384833e-49 | 4.019446e-46 |
| MsG0480018362.01 | MsG0880043998.01 | 0.853412 | 2.430249e-61 | 5.246586e-58 |
| MsG0580026492.01 | MsG0880043998.01 | 0.804037 | 2.617546e-49 | 1.335833e-46 |
| MsG0580026521.01 | MsG0880043998.01 | 0.802817 | 4.685232e-49 | 2.317490e-46 |
| MsG0580028302.01 | MsG0880043998.01 | 0.819405 | 1.181958e-52 | 9.086923e-50 |
| MsG0580028490.01 | MsG0880043998.01 | 0.818788 | 1.632959e-52 | 1.234183e-49 |
| MsG0680030613.01 | MsG0880043998.01 | 0.856412 | 3.273710e-62 | 7.807387e-59 |
| MsG0280007911.01 | MsG0880043998.01 | 0.812871 | 3.408001e-51 | 2.191609e-48 |
| MsG0280009432.01 | MsG0880043998.01 | 0.807287 | 5.441313e-50 | 3.018934e-47 |
| MsG0280009526.01 | MsG0880043998.01 | 0.813631 | 2.321366e-51 | 1.523924e-48 |
| MsG0280009657.01 | MsG0880043998.01 | 0.815248 | 1.018734e-51 | 6.986739e-49 |
| MsG0780036480.01 | MsG0880043998.01 | 0.806423 | 8.286934e-50 | 4.496118e-47 |
| MsG0780036839.01 | MsG0880043998.01 | 0.802387 | 5.748694e-49 | 2.812464e-46 |
| MsG0780038305.01 | MsG0880043998.01 | 0.815779 | 7.762378e-52 | 5.402381e-49 |
| MsG0780039237.01 | MsG0880043998.01 | 0.800196 | 1.614003e-48 | 7.469124e-46 |
| MsG0780039619.01 | MsG0880043998.01 | 0.815777 | 7.770136e-52 | 5.407487e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043998.01.T01 | MTR_8g445140 | 58.846 | 260 | 56 | 4 | 28 | 236 | 44 | 303 | 1.15e-100 | 295 |
| MsG0880043998.01.T01 | MTR_8g046000 | 59.633 | 218 | 87 | 1 | 32 | 249 | 20 | 236 | 2.14e-96 | 282 |
| MsG0880043998.01.T01 | MTR_1g045860 | 59.556 | 225 | 77 | 5 | 29 | 249 | 55 | 269 | 5.05e-89 | 265 |
| MsG0880043998.01.T01 | MTR_8g445170 | 71.264 | 174 | 48 | 1 | 78 | 249 | 224 | 397 | 1.53e-86 | 263 |
| MsG0880043998.01.T01 | MTR_8g045890 | 73.292 | 161 | 43 | 0 | 89 | 249 | 285 | 445 | 2.90e-86 | 263 |
| MsG0880043998.01.T01 | MTR_8g045890 | 73.292 | 161 | 43 | 0 | 89 | 249 | 326 | 486 | 1.01e-85 | 263 |
| MsG0880043998.01.T01 | MTR_8g045890 | 54.098 | 61 | 28 | 0 | 28 | 88 | 15 | 75 | 4.66e-14 | 71.6 |
| MsG0880043998.01.T01 | MTR_8g045890 | 73.292 | 161 | 43 | 0 | 89 | 249 | 363 | 523 | 2.40e-85 | 263 |
| MsG0880043998.01.T01 | MTR_8g045890 | 54.098 | 61 | 28 | 0 | 28 | 88 | 52 | 112 | 4.45e-14 | 71.6 |
| MsG0880043998.01.T01 | MTR_8g045880 | 77.500 | 160 | 36 | 0 | 90 | 249 | 169 | 328 | 4.32e-84 | 254 |
| MsG0880043998.01.T01 | MTR_8g045880 | 53.623 | 69 | 32 | 0 | 28 | 96 | 15 | 83 | 3.00e-16 | 77.0 |
| MsG0880043998.01.T01 | MTR_8g045880 | 77.500 | 160 | 36 | 0 | 90 | 249 | 204 | 363 | 1.38e-83 | 254 |
| MsG0880043998.01.T01 | MTR_8g045880 | 53.623 | 69 | 32 | 0 | 28 | 96 | 50 | 118 | 5.04e-16 | 76.6 |
| MsG0880043998.01.T01 | MTR_8g045880 | 77.500 | 160 | 36 | 0 | 90 | 249 | 206 | 365 | 1.56e-83 | 254 |
| MsG0880043998.01.T01 | MTR_8g045880 | 53.623 | 69 | 32 | 0 | 28 | 96 | 52 | 120 | 6.43e-16 | 76.6 |
| MsG0880043998.01.T01 | MTR_8g044290 | 68.243 | 148 | 43 | 2 | 64 | 209 | 195 | 340 | 3.67e-66 | 212 |
| MsG0880043998.01.T01 | MTR_2g081610 | 47.549 | 204 | 83 | 4 | 42 | 236 | 72 | 260 | 2.66e-65 | 204 |
| MsG0880043998.01.T01 | MTR_2g081770 | 47.321 | 224 | 96 | 9 | 23 | 236 | 60 | 271 | 2.75e-63 | 199 |
| MsG0880043998.01.T01 | MTR_2g081590 | 46.040 | 202 | 85 | 4 | 44 | 236 | 74 | 260 | 2.27e-59 | 189 |
| MsG0880043998.01.T01 | MTR_3g109490 | 37.903 | 248 | 110 | 8 | 19 | 235 | 87 | 321 | 2.20e-43 | 149 |
| MsG0880043998.01.T01 | MTR_1g008470 | 38.571 | 210 | 107 | 7 | 44 | 233 | 90 | 297 | 3.95e-40 | 140 |
| MsG0880043998.01.T01 | MTR_1g008590 | 36.441 | 236 | 125 | 8 | 19 | 234 | 93 | 323 | 8.55e-39 | 137 |
| MsG0880043998.01.T01 | MTR_0312s0030 | 36.017 | 236 | 126 | 8 | 19 | 234 | 93 | 323 | 7.72e-38 | 135 |
| MsG0880043998.01.T01 | MTR_1g007520 | 36.017 | 236 | 126 | 8 | 19 | 234 | 93 | 323 | 7.72e-38 | 135 |
| MsG0880043998.01.T01 | MTR_1g008460 | 36.596 | 235 | 129 | 10 | 15 | 233 | 64 | 294 | 1.20e-37 | 134 |
| MsG0880043998.01.T01 | MTR_1g008510 | 37.788 | 217 | 116 | 8 | 37 | 234 | 108 | 324 | 3.36e-37 | 133 |
| MsG0880043998.01.T01 | MTR_1g008560 | 36.652 | 221 | 117 | 8 | 37 | 234 | 108 | 328 | 1.80e-36 | 131 |
| MsG0880043998.01.T01 | MTR_1g008500 | 34.874 | 238 | 128 | 8 | 19 | 234 | 151 | 383 | 3.31e-36 | 132 |
| MsG0880043998.01.T01 | MTR_1g008530 | 43.836 | 146 | 75 | 4 | 96 | 234 | 77 | 222 | 3.39e-36 | 128 |
| MsG0880043998.01.T01 | MTR_1g008580 | 43.243 | 148 | 77 | 4 | 96 | 236 | 25 | 172 | 4.43e-36 | 126 |
| MsG0880043998.01.T01 | MTR_1g008450 | 33.484 | 221 | 118 | 8 | 37 | 233 | 95 | 310 | 6.32e-31 | 116 |
| MsG0880043998.01.T01 | MTR_3g116380 | 39.286 | 140 | 76 | 5 | 105 | 235 | 163 | 302 | 6.89e-22 | 92.4 |
| MsG0880043998.01.T01 | MTR_4g069680 | 32.420 | 219 | 118 | 9 | 44 | 235 | 71 | 286 | 6.18e-21 | 89.7 |
| MsG0880043998.01.T01 | MTR_3g107830 | 36.486 | 148 | 84 | 4 | 98 | 235 | 108 | 255 | 1.52e-20 | 88.2 |
| MsG0880043998.01.T01 | MTR_3g116270 | 32.558 | 215 | 123 | 8 | 43 | 235 | 291 | 505 | 2.29e-19 | 87.0 |
| MsG0880043998.01.T01 | MTR_3g116410 | 36.913 | 149 | 84 | 5 | 98 | 236 | 128 | 276 | 3.03e-19 | 84.7 |
| MsG0880043998.01.T01 | MTR_3g116320 | 32.558 | 215 | 123 | 8 | 43 | 235 | 473 | 687 | 7.03e-19 | 85.9 |
| MsG0880043998.01.T01 | MTR_4g069570 | 34.000 | 150 | 87 | 7 | 98 | 235 | 186 | 335 | 2.02e-17 | 80.5 |
| MsG0880043998.01.T01 | MTR_4g069550 | 34.000 | 150 | 87 | 6 | 98 | 235 | 313 | 462 | 2.74e-16 | 78.2 |
| MsG0880043998.01.T01 | MTR_4g069040 | 33.566 | 143 | 83 | 7 | 105 | 235 | 367 | 509 | 2.74e-16 | 78.2 |
| MsG0880043998.01.T01 | MTR_8g064500 | 33.784 | 148 | 88 | 5 | 96 | 234 | 225 | 371 | 4.89e-15 | 73.9 |
| MsG0880043998.01.T01 | MTR_8g064560 | 34.247 | 146 | 86 | 6 | 98 | 234 | 480 | 624 | 1.04e-13 | 70.5 |
| MsG0880043998.01.T01 | MTR_4g133840 | 29.655 | 145 | 93 | 4 | 98 | 233 | 42 | 186 | 4.79e-13 | 66.2 |
| MsG0880043998.01.T01 | MTR_8g064530 | 29.744 | 195 | 110 | 8 | 65 | 233 | 447 | 640 | 1.81e-12 | 67.0 |
| MsG0880043998.01.T01 | MTR_5g034320 | 30.345 | 145 | 93 | 4 | 98 | 234 | 483 | 627 | 2.97e-12 | 66.2 |
| MsG0880043998.01.T01 | MTR_4g068420 | 31.061 | 132 | 72 | 6 | 110 | 235 | 2 | 120 | 1.49e-11 | 60.5 |
| MsG0880043998.01.T01 | MTR_6g086530 | 29.861 | 144 | 89 | 6 | 105 | 236 | 63 | 206 | 8.33e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043998.01.T01 | AT5G25610 | 34.711 | 242 | 123 | 10 | 19 | 233 | 156 | 389 | 2.42e-36 | 132 |
| MsG0880043998.01.T01 | AT1G49320 | 31.174 | 247 | 131 | 12 | 22 | 234 | 37 | 278 | 6.93e-20 | 86.7 |
| MsG0880043998.01.T01 | AT1G70370 | 31.034 | 145 | 92 | 4 | 98 | 234 | 479 | 623 | 2.35e-15 | 75.5 |
| MsG0880043998.01.T01 | AT1G70370 | 31.034 | 145 | 92 | 4 | 98 | 234 | 479 | 623 | 2.35e-15 | 75.5 |
| MsG0880043998.01.T01 | AT1G23760 | 30.137 | 146 | 92 | 5 | 98 | 234 | 475 | 619 | 2.92e-13 | 69.3 |
| MsG0880043998.01.T01 | AT1G60390 | 31.507 | 146 | 92 | 5 | 96 | 233 | 475 | 620 | 3.90e-13 | 68.9 |
Find 55 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTATATATTGGTCTTGGTTT+TGG | 0.131953 | 8:+34104234 | None:intergenic |
| GTAGAGGAAGTGAAGAAAAT+TGG | 0.216702 | 8:-34104212 | MsG0880043998.01.T01:CDS |
| CTTGTCAAATTGATCACTAA+TGG | 0.289242 | 8:+34104553 | None:intergenic |
| GAAATCTATCATTGATTCTA+AGG | 0.310409 | 8:+34104307 | None:intergenic |
| ATCAATTTGACAAGCAAGAT+TGG | 0.320032 | 8:-34104544 | MsG0880043998.01.T01:CDS |
| GTGTGAACCCAGCAGCTATA+GGG | 0.326521 | 8:-34104352 | MsG0880043998.01.T01:CDS |
| TTGTGTTCCCCTATAGCTGC+TGG | 0.326965 | 8:+34104344 | None:intergenic |
| TTGCTAACAAAATGACATAT+TGG | 0.335569 | 8:+34103984 | None:intergenic |
| TGGAAGAATAAGACAACCTT+TGG | 0.342882 | 8:-34104417 | MsG0880043998.01.T01:CDS |
| TCACTTCCTCTACTATATAT+TGG | 0.350269 | 8:+34104222 | None:intergenic |
| TGACATATTGGAATTGTTCC+AGG | 0.361803 | 8:+34103996 | None:intergenic |
| CCTCTACTATATATTGGTCT+TGG | 0.371712 | 8:+34104228 | None:intergenic |
| GTAGAGGTCATGTTGGAAAA+AGG | 0.374297 | 8:+34104517 | None:intergenic |
| ATTTGTTCCAAGCTTTGAAA+TGG | 0.389440 | 8:+34104283 | None:intergenic |
| GATTTCGCCATTTCAAAGCT+TGG | 0.391238 | 8:-34104290 | MsG0880043998.01.T01:CDS |
| AACCTTTGGGAACATGGATA+TGG | 0.395939 | 8:-34104403 | MsG0880043998.01.T01:CDS |
| AACATACATAGTTCCTTTAG+TGG | 0.396577 | 8:-34104114 | MsG0880043998.01.T01:CDS |
| TTGTCAAATTGATCACTAAT+GGG | 0.408713 | 8:+34104554 | None:intergenic |
| CCAACAGGTATCCATGCATT+TGG | 0.413169 | 8:+34104437 | None:intergenic |
| AGACTCACTTACAACATGAT+TGG | 0.419700 | 8:+34103941 | None:intergenic |
| GGTTGTCTTATTCTTCCAAC+AGG | 0.420121 | 8:+34104422 | None:intergenic |
| TGTGTGAACCCAGCAGCTAT+AGG | 0.462266 | 8:-34104353 | MsG0880043998.01.T01:CDS |
| CTTAGGCTTCCACAAGCATT+CGG | 0.462551 | 8:-34104477 | MsG0880043998.01.T01:CDS |
| GACTCACTTACAACATGATT+GGG | 0.472431 | 8:+34103942 | None:intergenic |
| AGAACTTCATAAAGCACGTC+AGG | 0.473905 | 8:+34104029 | None:intergenic |
| GTTCCATCAGAGGCCACTAA+AGG | 0.489886 | 8:+34104101 | None:intergenic |
| TGTGTTCCCCTATAGCTGCT+GGG | 0.500356 | 8:+34104345 | None:intergenic |
| GTTCCTTTAGTGGCCTCTGA+TGG | 0.500787 | 8:-34104104 | MsG0880043998.01.T01:CDS |
| TTCCAACATGACCTCTACCC+TGG | 0.507075 | 8:-34104512 | MsG0880043998.01.T01:CDS |
| TTGATGTTGCATTGACTTGG+TGG | 0.512892 | 8:+34104135 | None:intergenic |
| GAAGTTCTCAAAGTCAAGCC+TGG | 0.515439 | 8:-34104014 | MsG0880043998.01.T01:CDS |
| TAAGACAACCTTTGGGAACA+TGG | 0.519820 | 8:-34104409 | MsG0880043998.01.T01:CDS |
| CCACCATGACACGAGAGGTA+TGG | 0.526766 | 8:-34104054 | MsG0880043998.01.T01:CDS |
| CTTTGCTTTAGTTCCATCAG+AGG | 0.537135 | 8:+34104091 | None:intergenic |
| GTTGCCTATCCGAATGCTTG+TGG | 0.538073 | 8:+34104468 | None:intergenic |
| CCTGGAAAGAAAATGAGCTT+AGG | 0.538447 | 8:-34104494 | MsG0880043998.01.T01:CDS |
| ATGTTGATGTTGCATTGACT+TGG | 0.542701 | 8:+34104132 | None:intergenic |
| GGAAGAATAAGACAACCTTT+GGG | 0.545608 | 8:-34104416 | MsG0880043998.01.T01:CDS |
| ATCCATGCATTTGGTCGTAA+CGG | 0.555667 | 8:+34104446 | None:intergenic |
| TTCCAGGGTAGAGGTCATGT+TGG | 0.557459 | 8:+34104510 | None:intergenic |
| TGTGAACCCAGCAGCTATAG+GGG | 0.559123 | 8:-34104351 | MsG0880043998.01.T01:CDS |
| ATTTAGTTTATGACACATCA+CGG | 0.560330 | 8:+34104181 | None:intergenic |
| GATCCATACCTCTCGTGTCA+TGG | 0.564641 | 8:+34104051 | None:intergenic |
| CTCCATATCCATGTTCCCAA+AGG | 0.572259 | 8:+34104401 | None:intergenic |
| CCAAATGCATGGATACCTGT+TGG | 0.574421 | 8:-34104437 | MsG0880043998.01.T01:CDS |
| TTAGCAACAAGGCCATTGCA+TGG | 0.576149 | 8:-34103968 | MsG0880043998.01.T01:CDS |
| TAGCAACAAGGCCATTGCAT+GGG | 0.577381 | 8:-34103967 | MsG0880043998.01.T01:CDS |
| CTCTGATGGAACTAAAGCAA+AGG | 0.599934 | 8:-34104090 | MsG0880043998.01.T01:CDS |
| GCTTCCACAAGCATTCGGAT+AGG | 0.612238 | 8:-34104472 | MsG0880043998.01.T01:CDS |
| AACCGTTACGACCAAATGCA+TGG | 0.613823 | 8:-34104448 | MsG0880043998.01.T01:CDS |
| ATGATTGGGTACCCATGCAA+TGG | 0.623581 | 8:+34103956 | None:intergenic |
| CCAAGACCAATATATAGTAG+AGG | 0.626479 | 8:-34104228 | MsG0880043998.01.T01:CDS |
| CCATACCTCTCGTGTCATGG+TGG | 0.655956 | 8:+34104054 | None:intergenic |
| GACACAGATTATATGACACA+AGG | 0.694555 | 8:+34103909 | None:intergenic |
| ATATGCCACCATGACACGAG+AGG | 0.699219 | 8:-34104059 | MsG0880043998.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTCTTTTAATTTGAAATTT+TGG | - | Chr8:34104634-34104653 | MsG0880043998.01.T01:intron | 10.0% |
| !! | ATATTAGATTCAAATTGTAA+CGG | + | Chr8:34104436-34104455 | None:intergenic | 15.0% |
| !! | AAATATAGACAATCAAAAAG+TGG | + | Chr8:34104778-34104797 | None:intergenic | 20.0% |
| !! | AATATAGACAATCAAAAAGT+GGG | + | Chr8:34104777-34104796 | None:intergenic | 20.0% |
| !! | ACTTCATAAATAAATGAAAC+AGG | - | Chr8:34104301-34104320 | MsG0880043998.01.T01:CDS | 20.0% |
| !! | CTTCATAAATAAATGAAACA+GGG | - | Chr8:34104302-34104321 | MsG0880043998.01.T01:CDS | 20.0% |
| !!! | ATGTATATATTTTTCAAGGT+TGG | + | Chr8:34104273-34104292 | None:intergenic | 20.0% |
| !!! | CATGATGTATATATTTTTCA+AGG | + | Chr8:34104277-34104296 | None:intergenic | 20.0% |
| !!! | GAAAAAGTAATTCAGAATTT+TGG | + | Chr8:34104618-34104637 | None:intergenic | 20.0% |
| ! | CGTCTCGATAAAAATTAAAT+TGG | + | Chr8:34104211-34104230 | None:intergenic | 25.0% |
| ! | TTAGAATCTTTAACATGTGT+AGG | - | Chr8:34104944-34104963 | MsG0880043998.01.T01:intron | 25.0% |
| ! | TTGCTAACAAAATGACATAT+TGG | + | Chr8:34105636-34105655 | None:intergenic | 25.0% |
| ! | TTGTCAAATTGATCACTAAT+GGG | + | Chr8:34105066-34105085 | None:intergenic | 25.0% |
| !! | AAGTCATAAACCTTTTAACA+TGG | + | Chr8:34104568-34104587 | None:intergenic | 25.0% |
| !! | AGTCATAAACCTTTTAACAT+GGG | + | Chr8:34104567-34104586 | None:intergenic | 25.0% |
| !! | ATTTAGTTTATGACACATCA+CGG | + | Chr8:34105439-34105458 | None:intergenic | 25.0% |
| !!! | ATTATTTTGTAAGCTTTCGT+TGG | - | Chr8:34104477-34104496 | MsG0880043998.01.T01:CDS | 25.0% |
| !!! | GAAATCTATCATTGATTCTA+AGG | + | Chr8:34105313-34105332 | None:intergenic | 25.0% |
| !!! | TATATATTGGTCTTGGTTTT+GGG | + | Chr8:34105385-34105404 | None:intergenic | 25.0% |
| !!! | TTATTTTGTAAGCTTTCGTT+GGG | - | Chr8:34104478-34104497 | MsG0880043998.01.T01:CDS | 25.0% |
| AACATACATAGTTCCTTTAG+TGG | - | Chr8:34105503-34105522 | MsG0880043998.01.T01:intron | 30.0% | |
| AACCTTGAACATTGAAATCA+TGG | + | Chr8:34104824-34104843 | None:intergenic | 30.0% | |
| AACTATGAGGTTAGAATCAT+TGG | + | Chr8:34104353-34104372 | None:intergenic | 30.0% | |
| AAGAATATTGGAAATCCGTT+TGG | - | Chr8:34104699-34104718 | MsG0880043998.01.T01:intron | 30.0% | |
| AATTCTATTGAAAACAGACG+TGG | + | Chr8:34104050-34104069 | None:intergenic | 30.0% | |
| ATCAATTTGACAAGCAAGAT+TGG | - | Chr8:34105073-34105092 | MsG0880043998.01.T01:intron | 30.0% | |
| CACAATCTGAAGAAGAATAT+TGG | - | Chr8:34104687-34104706 | MsG0880043998.01.T01:intron | 30.0% | |
| CTTGTCAAATTGATCACTAA+TGG | + | Chr8:34105067-34105086 | None:intergenic | 30.0% | |
| GATAAACTAATAGCTAGTGA+TGG | + | Chr8:34104400-34104419 | None:intergenic | 30.0% | |
| TCACTTCCTCTACTATATAT+TGG | + | Chr8:34105398-34105417 | None:intergenic | 30.0% | |
| ! | GTCATAAACCTTTTAACATG+GGG | + | Chr8:34104566-34104585 | None:intergenic | 30.0% |
| ! | TATTTTGTAAGCTTTCGTTG+GGG | - | Chr8:34104479-34104498 | MsG0880043998.01.T01:CDS | 30.0% |
| ! | TCACTAGCTATTAGTTTATC+TGG | - | Chr8:34104400-34104419 | MsG0880043998.01.T01:CDS | 30.0% |
| !! | AAAAAGTGTCTCTAACTATG+AGG | + | Chr8:34104366-34104385 | None:intergenic | 30.0% |
| !! | ATTTGTTCCAAGCTTTGAAA+TGG | + | Chr8:34105337-34105356 | None:intergenic | 30.0% |
| !!! | ATGTCATTTTGTTAGCAACA+AGG | - | Chr8:34105638-34105657 | MsG0880043998.01.T01:intron | 30.0% |
| !!! | CTATATATTGGTCTTGGTTT+TGG | + | Chr8:34105386-34105405 | None:intergenic | 30.0% |
| !!! | TATTTTTCAAGGTTGGAAAG+AGG | + | Chr8:34104266-34104285 | None:intergenic | 30.0% |
| AGACTCACTTACAACATGAT+TGG | + | Chr8:34105679-34105698 | None:intergenic | 35.0% | |
| ATTCCTTCCTGAAAAGAGTA+AGG | + | Chr8:34104027-34104046 | None:intergenic | 35.0% | |
| CCAAGACCAATATATAGTAG+AGG | - | Chr8:34105389-34105408 | MsG0880043998.01.T01:intron | 35.0% | |
| GACTCACTTACAACATGATT+GGG | + | Chr8:34105678-34105697 | None:intergenic | 35.0% | |
| GAGTCAAATCAGATTCAACT+CGG | + | Chr8:34104236-34104255 | None:intergenic | 35.0% | |
| GGAAGAATAAGACAACCTTT+GGG | - | Chr8:34105201-34105220 | MsG0880043998.01.T01:intron | 35.0% | |
| GTAGAGGAAGTGAAGAAAAT+TGG | - | Chr8:34105405-34105424 | MsG0880043998.01.T01:intron | 35.0% | |
| TGACATATTGGAATTGTTCC+AGG | + | Chr8:34105624-34105643 | None:intergenic | 35.0% | |
| TGCCATGATTTCAATGTTCA+AGG | - | Chr8:34104819-34104838 | MsG0880043998.01.T01:intron | 35.0% | |
| TGGAAGAATAAGACAACCTT+TGG | - | Chr8:34105200-34105219 | MsG0880043998.01.T01:intron | 35.0% | |
| ! | CCTCTACTATATATTGGTCT+TGG | + | Chr8:34105392-34105411 | None:intergenic | 35.0% |
| ! | CTAAGCTCATTTTCTTTCCA+GGG | + | Chr8:34105125-34105144 | None:intergenic | 35.0% |
| ! | GAGTTGAATCTGATTTGACT+CGG | - | Chr8:34104235-34104254 | MsG0880043998.01.T01:CDS | 35.0% |
| ! | TTTGGTGTAGTTAACTCTTG+TGG | - | Chr8:34104652-34104671 | MsG0880043998.01.T01:intron | 35.0% |
| !! | ATGTTGATGTTGCATTGACT+TGG | + | Chr8:34105488-34105507 | None:intergenic | 35.0% |
| !! | CTTTTTCACCCCATGTTAAA+AGG | - | Chr8:34104555-34104574 | MsG0880043998.01.T01:CDS | 35.0% |
| AGAACTTCATAAAGCACGTC+AGG | + | Chr8:34105591-34105610 | None:intergenic | 40.0% | |
| ATCCATGCATTTGGTCGTAA+CGG | + | Chr8:34105174-34105193 | None:intergenic | 40.0% | |
| ATTCAAGCTCACGAACAACT+CGG | + | Chr8:34104132-34104151 | None:intergenic | 40.0% | |
| CCTGAAAAGAGTAAGGTAGT+AGG | + | Chr8:34104020-34104039 | None:intergenic | 40.0% | |
| CCTGGAAAGAAAATGAGCTT+AGG | - | Chr8:34105123-34105142 | MsG0880043998.01.T01:intron | 40.0% | |
| CTCTGATGGAACTAAAGCAA+AGG | - | Chr8:34105527-34105546 | MsG0880043998.01.T01:intron | 40.0% | |
| GATTTCGCCATTTCAAAGCT+TGG | - | Chr8:34105327-34105346 | MsG0880043998.01.T01:intron | 40.0% | |
| GTAGAGGTCATGTTGGAAAA+AGG | + | Chr8:34105103-34105122 | None:intergenic | 40.0% | |
| TCTCCTCGACCTGTTTATTT+CGG | - | Chr8:34104745-34104764 | MsG0880043998.01.T01:intron | 40.0% | |
| ! | AACCTTTGGGAACATGGATA+TGG | - | Chr8:34105214-34105233 | MsG0880043998.01.T01:intron | 40.0% |
| ! | CCTAAGCTCATTTTCTTTCC+AGG | + | Chr8:34105126-34105145 | None:intergenic | 40.0% |
| ! | CCTACTACCTTACTCTTTTC+AGG | - | Chr8:34104017-34104036 | MsG0880043998.01.T01:CDS | 40.0% |
| ! | CTTTGCTTTAGTTCCATCAG+AGG | + | Chr8:34105529-34105548 | None:intergenic | 40.0% |
| ! | CTTTTCAACCTACTTGCGAA+GGG | - | Chr8:34104590-34104609 | MsG0880043998.01.T01:CDS | 40.0% |
| ! | GGTTGTCTTATTCTTCCAAC+AGG | + | Chr8:34105198-34105217 | None:intergenic | 40.0% |
| ! | TAAGACAACCTTTGGGAACA+TGG | - | Chr8:34105208-34105227 | MsG0880043998.01.T01:intron | 40.0% |
| ! | TCATTTTCTTTCCAGGGTAG+AGG | + | Chr8:34105119-34105138 | None:intergenic | 40.0% |
| ! | TTTTCAACCTACTTGCGAAG+GGG | - | Chr8:34104591-34104610 | MsG0880043998.01.T01:CDS | 40.0% |
| !! | CTACCTTACTCTTTTCAGGA+AGG | - | Chr8:34104021-34104040 | MsG0880043998.01.T01:CDS | 40.0% |
| !! | TTGATGTTGCATTGACTTGG+TGG | + | Chr8:34105485-34105504 | None:intergenic | 40.0% |
| AACCCGAAATAAACAGGTCG+AGG | + | Chr8:34104751-34104770 | None:intergenic | 45.0% | |
| AACCGTTACGACCAAATGCA+TGG | - | Chr8:34105169-34105188 | MsG0880043998.01.T01:intron | 45.0% | |
| ATGATTGGGTACCCATGCAA+TGG | + | Chr8:34105664-34105683 | None:intergenic | 45.0% | |
| CCAAATGCATGGATACCTGT+TGG | - | Chr8:34105180-34105199 | MsG0880043998.01.T01:intron | 45.0% | |
| CCAACAGGTATCCATGCATT+TGG | + | Chr8:34105183-34105202 | None:intergenic | 45.0% | |
| CTCCATATCCATGTTCCCAA+AGG | + | Chr8:34105219-34105238 | None:intergenic | 45.0% | |
| CTCCTCGACCTGTTTATTTC+GGG | - | Chr8:34104746-34104765 | MsG0880043998.01.T01:intron | 45.0% | |
| CTTAGGCTTCCACAAGCATT+CGG | - | Chr8:34105140-34105159 | MsG0880043998.01.T01:intron | 45.0% | |
| GAAGTTCTCAAAGTCAAGCC+TGG | - | Chr8:34105603-34105622 | MsG0880043998.01.T01:intron | 45.0% | |
| GGGTCTAACCCGAAATAAAC+AGG | + | Chr8:34104757-34104776 | None:intergenic | 45.0% | |
| TAGCAACAAGGCCATTGCAT+GGG | - | Chr8:34105650-34105669 | MsG0880043998.01.T01:intron | 45.0% | |
| TAGCTAGTGATGGCAGTAGT+TGG | + | Chr8:34104390-34104409 | None:intergenic | 45.0% | |
| TTAGCAACAAGGCCATTGCA+TGG | - | Chr8:34105649-34105668 | MsG0880043998.01.T01:intron | 45.0% | |
| ! | CCTTCGCAAGTAGGTTGAAA+AGG | + | Chr8:34104592-34104611 | None:intergenic | 45.0% |
| ! | CCTTTTCAACCTACTTGCGA+AGG | - | Chr8:34104589-34104608 | MsG0880043998.01.T01:CDS | 45.0% |
| !! | CATAGGAGTGTTTGGCCAAA+CGG | + | Chr8:34104717-34104736 | None:intergenic | 45.0% |
| !!! | TTGGTCTTGGTTTTGGGCAA+AGG | + | Chr8:34105379-34105398 | None:intergenic | 45.0% |
| !!! | ATAATACTTTTTATTTTTAA+TGG | - | Chr8:34105018-34105037 | MsG0880043998.01.T01:intron | 5.0% |
| AACAGGTCGAGGAGAGTCTT+CGG | + | Chr8:34104740-34104759 | None:intergenic | 50.0% | |
| ATATGCCACCATGACACGAG+AGG | - | Chr8:34105558-34105577 | MsG0880043998.01.T01:intron | 50.0% | |
| GATCCATACCTCTCGTGTCA+TGG | + | Chr8:34105569-34105588 | None:intergenic | 50.0% | |
| GCTTCCACAAGCATTCGGAT+AGG | - | Chr8:34105145-34105164 | MsG0880043998.01.T01:intron | 50.0% | |
| GTGTGAACCCAGCAGCTATA+GGG | - | Chr8:34105265-34105284 | MsG0880043998.01.T01:intron | 50.0% | |
| GTTCCATCAGAGGCCACTAA+AGG | + | Chr8:34105519-34105538 | None:intergenic | 50.0% | |
| GTTCCTTTAGTGGCCTCTGA+TGG | - | Chr8:34105513-34105532 | MsG0880043998.01.T01:intron | 50.0% | |
| GTTGCCTATCCGAATGCTTG+TGG | + | Chr8:34105152-34105171 | None:intergenic | 50.0% | |
| TGTGAACCCAGCAGCTATAG+GGG | - | Chr8:34105266-34105285 | MsG0880043998.01.T01:intron | 50.0% | |
| TGTGTGAACCCAGCAGCTAT+AGG | - | Chr8:34105264-34105283 | MsG0880043998.01.T01:intron | 50.0% | |
| TGTGTTCCCCTATAGCTGCT+GGG | + | Chr8:34105275-34105294 | None:intergenic | 50.0% | |
| TTCCAACATGACCTCTACCC+TGG | - | Chr8:34105105-34105124 | MsG0880043998.01.T01:intron | 50.0% | |
| TTCCAGGGTAGAGGTCATGT+TGG | + | Chr8:34105110-34105129 | None:intergenic | 50.0% | |
| TTGTGTTCCCCTATAGCTGC+TGG | + | Chr8:34105276-34105295 | None:intergenic | 50.0% | |
| ! | TTTTGGTCCCCTTCGCAAGT+AGG | + | Chr8:34104601-34104620 | None:intergenic | 50.0% |
| !! | GTCTTCGGCATAGGAGTGTT+TGG | + | Chr8:34104725-34104744 | None:intergenic | 50.0% |
| CCACCATGACACGAGAGGTA+TGG | - | Chr8:34105563-34105582 | MsG0880043998.01.T01:intron | 55.0% | |
| CCATACCTCTCGTGTCATGG+TGG | + | Chr8:34105566-34105585 | None:intergenic | 55.0% | |
| TCGAGGAGAGTCTTCGGCAT+AGG | + | Chr8:34104734-34104753 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 34103917 | 34105722 | 34103917 | ID=MsG0880043998.01;Name=MsG0880043998.01 |
| Chr8 | mRNA | 34103917 | 34105722 | 34103917 | ID=MsG0880043998.01.T01;Parent=MsG0880043998.01;Name=MsG0880043998.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|0|0|2|0|249 |
| Chr8 | exon | 34105681 | 34105722 | 34105681 | ID=MsG0880043998.01.T01:exon:10481;Parent=MsG0880043998.01.T01 |
| Chr8 | exon | 34103917 | 34104624 | 34103917 | ID=MsG0880043998.01.T01:exon:10480;Parent=MsG0880043998.01.T01 |
| Chr8 | CDS | 34105681 | 34105722 | 34105681 | ID=MsG0880043998.01.T01:cds;Parent=MsG0880043998.01.T01 |
| Chr8 | CDS | 34103917 | 34104624 | 34103917 | ID=MsG0880043998.01.T01:cds;Parent=MsG0880043998.01.T01 |
| Gene Sequence |
| Protein sequence |
