AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021227.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021227.01.T01 MTR_4g069300 92.837 363 26 0 31 393 76 438 0.0 706
MsG0480021227.01.T01 MTR_4g069260 87.097 372 47 1 22 393 67 437 0.0 685
MsG0480021227.01.T01 MTR_4g069350 83.073 384 56 2 8 391 55 429 0.0 646
MsG0480021227.01.T01 MTR_4g069420 79.948 384 66 2 8 391 55 427 0.0 627
MsG0480021227.01.T01 MTR_4g069430 83.784 370 44 2 22 391 67 420 0.0 624
MsG0480021227.01.T01 MTR_4g069390 83.754 357 53 1 35 391 1 352 0.0 623
MsG0480021227.01.T01 MTR_1g022025 73.913 368 95 1 24 391 61 427 0.0 583
MsG0480021227.01.T01 MTR_8g069400 28.351 194 110 8 121 306 1390 1562 6.44e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021227.01.T01 AT1G19360 72.368 380 103 2 14 392 50 428 0.0 574
MsG0480021227.01.T01 AT1G19360 72.368 380 103 2 14 392 50 428 0.0 574
MsG0480021227.01.T01 AT1G75110 72.207 367 101 1 25 391 62 427 0.0 566
MsG0480021227.01.T01 AT1G75120 66.848 368 109 3 24 391 48 402 0.0 509

Find 87 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCTTCAATTGAGCTTT+TGG 0.172013 4:-55385111 MsG0480021227.01.T01:CDS
AGGATATGGGTTTACAATTC+TGG 0.249487 4:-55385155 MsG0480021227.01.T01:CDS
TTCATTCGCCGAAGCTTATC+AGG 0.266822 4:+55384867 None:intergenic
CATAATAACATGACAGCTTA+TGG 0.285401 4:-55385236 MsG0480021227.01.T01:CDS
GGAGGGAGAGCAGCTGTCTC+TGG 0.299812 4:-55385395 MsG0480021227.01.T01:CDS
TAGTTCATACCACGAGGATA+TGG 0.304296 4:-55385169 MsG0480021227.01.T01:CDS
AAGGCAACAACTAGATAATT+AGG 0.329864 4:+55385509 None:intergenic
TCATGCTTCTAAAAGAACTA+TGG 0.336021 4:-55384991 MsG0480021227.01.T01:CDS
GCTTCTTGCATTAGGTAAAC+AGG 0.362356 4:-55385729 MsG0480021227.01.T01:CDS
TCTAATATATTTGCCAATCT+CGG 0.386117 4:+55385632 None:intergenic
TAATTCACGTGAATAATTAC+CGG 0.389710 4:+55384894 None:intergenic
GTTGTTGATTATTATGTCAA+TGG 0.405758 4:-55384840 MsG0480021227.01.T01:CDS
TATACCGACTCTCTTGATAT+TGG 0.413611 4:+55385532 None:intergenic
AAAGAGTTCTTCATTGAAAA+CGG 0.417137 4:+55385043 None:intergenic
AACAGTAGGATTGGTTCTTA+AGG 0.420894 4:+55385676 None:intergenic
GGTTACCACTTTGAGCCATC+AGG 0.421929 4:+55384792 None:intergenic
CTAATATATTTGCCAATCTC+GGG 0.422456 4:+55385633 None:intergenic
CCTTGCAAACTCCAATGTTA+AGG 0.426563 4:-55385576 MsG0480021227.01.T01:CDS
CATGTTAAAATCAGAATTTG+TGG 0.440337 4:-55385852 MsG0480021227.01.T01:CDS
TTGTTGATTATTATGTCAAT+GGG 0.453648 4:-55384839 MsG0480021227.01.T01:CDS
TTGGTCCCATGCTTTCTCCT+TGG 0.456387 4:+55385067 None:intergenic
CCGTCCCATCCTGGTTATGA+CGG 0.460145 4:-55385017 MsG0480021227.01.T01:CDS
CTCGGGTTCACGGATTCATC+AGG 0.461693 4:+55385650 None:intergenic
AGCTCAATTGAAGGAATGGT+TGG 0.470800 4:+55385116 None:intergenic
ACAGTATCAATACCATCATC+CGG 0.475603 4:+55385428 None:intergenic
AGTTCATACCACGAGGATAT+GGG 0.478586 4:-55385168 MsG0480021227.01.T01:CDS
TGAATCCGTGAACCCGAGAT+TGG 0.481653 4:-55385645 MsG0480021227.01.T01:CDS
ATACTCTCCGGATACGTATC+GGG 0.483540 4:+55386913 None:intergenic
TTAAGGAGACCCTCGAGGTC+TGG 0.486077 4:-55385559 MsG0480021227.01.T01:CDS
ATCAGGAGCAACAGTAGGAT+TGG 0.486219 4:+55385667 None:intergenic
CAAAAGCTCAATTGAAGGAA+TGG 0.491186 4:+55385112 None:intergenic
GGAAAGGTTTGAGAGCATCT+TGG 0.491288 4:+55384814 None:intergenic
TACAAAAGAGACCCGGATGA+TGG 0.496519 4:-55385440 MsG0480021227.01.T01:CDS
GATACGTATCGGGAAGTATC+GGG 0.496818 4:+55386923 None:intergenic
TAACCTCAGTAAATTAAAAC+CGG 0.496942 4:-55384913 MsG0480021227.01.T01:CDS
CACTTTGAGCCATCAGGGAA+AGG 0.502373 4:+55384798 None:intergenic
CGATCCAAAAGCTCAATTGA+AGG 0.505035 4:+55385107 None:intergenic
TCACGGTATAGATGATCAAA+AGG 0.505121 4:+55385287 None:intergenic
ATTTGCCAATCTCGGGTTCA+CGG 0.510225 4:+55385640 None:intergenic
CTTTGAAATTTCCTCGTCGA+AGG 0.515983 4:+55385490 None:intergenic
CCTTAACATTGGAGTTTGCA+AGG 0.516596 4:+55385576 None:intergenic
GAGGGAGAGCAGCTGTCTCT+GGG 0.517475 4:-55385394 MsG0480021227.01.T01:CDS
AGGCTTCAGCTTGCAGAGCA+AGG 0.524017 4:-55385773 MsG0480021227.01.T01:CDS
GGCTTTCCAAGGAGAAAGCA+TGG 0.525521 4:-55385073 MsG0480021227.01.T01:CDS
CGTCCCATCCTGGTTATGAC+GGG 0.529822 4:-55385016 MsG0480021227.01.T01:CDS
GTTCTTCATTGAAAACGGCT+TGG 0.536649 4:+55385048 None:intergenic
ATTACCATCCTGATAAGCTT+CGG 0.537140 4:-55384875 MsG0480021227.01.T01:CDS
TGCAGGATACGTAGGGGATA+CGG 0.538432 4:+55386956 None:intergenic
TATCGGGCAGGTATCGGTGC+AGG 0.551317 4:+55386939 None:intergenic
GGGAAGTATCGGGCAGGTAT+CGG 0.552305 4:+55386933 None:intergenic
TATCTATGATGAACCCGTGA+TGG 0.554851 4:-55385204 MsG0480021227.01.T01:CDS
GGATACGTATCGGGAAGTAT+CGG 0.558361 4:+55386922 None:intergenic
GCATGAAGCCCGTCATAACC+AGG 0.559070 4:+55385008 None:intergenic
CTTAACATTGGAGTTTGCAA+GGG 0.559512 4:+55385577 None:intergenic
GCTCTCAAACCTTTCCCTGA+TGG 0.559523 4:-55384807 MsG0480021227.01.T01:CDS
GTTACCACTTTGAGCCATCA+GGG 0.562119 4:+55384793 None:intergenic
CTCGAGGGTCTCCTTAACAT+TGG 0.565730 4:+55385565 None:intergenic
GGTATCGGTGCAGGATACGT+AGG 0.586632 4:+55386948 None:intergenic
TAATCGAGACCAACCCATCA+CGG 0.595014 4:+55385190 None:intergenic
GAAGCCCGTCATAACCAGGA+TGG 0.597381 4:+55385012 None:intergenic
AATCGAGACCAACCCATCAC+GGG 0.597461 4:+55385191 None:intergenic
AAGGCCTTGACAGTACCAAA+AGG 0.598804 4:+55385695 None:intergenic
AAGCCCGTCATAACCAGGAT+GGG 0.599228 4:+55385013 None:intergenic
AGGTGAGTGAGTTGACAGAA+AGG 0.599718 4:-55385793 MsG0480021227.01.T01:CDS
GTATCGGTGCAGGATACGTA+GGG 0.599866 4:+55386949 None:intergenic
TGTCGCGACAAGGCTTTCCA+AGG 0.604324 4:-55385084 MsG0480021227.01.T01:CDS
TGCAGAGCAAGGAAAAGACC+AGG 0.607035 4:-55385762 MsG0480021227.01.T01:CDS
CGTATCGGGAAGTATCGGGC+AGG 0.609767 4:+55386927 None:intergenic
TTCGCCGAAGCTTATCAGGA+TGG 0.615270 4:+55384871 None:intergenic
CCGTCATAACCAGGATGGGA+CGG 0.618599 4:+55385017 None:intergenic
CAGTATCAATACCATCATCC+GGG 0.624695 4:+55385429 None:intergenic
GATTCATCAGGAGCAACAGT+AGG 0.626788 4:+55385662 None:intergenic
ATGATGAACCCGTGATGGGT+TGG 0.627261 4:-55385199 MsG0480021227.01.T01:CDS
ATCTATGATGAACCCGTGAT+GGG 0.629134 4:-55385203 MsG0480021227.01.T01:CDS
TTCACCAATATCAAGAGAGT+CGG 0.631376 4:-55385536 MsG0480021227.01.T01:CDS
GATGATGGTATTGATACTGT+TGG 0.643432 4:-55385425 MsG0480021227.01.T01:CDS
TTGACTCAACATCTGAATCA+CGG 0.644066 4:+55385270 None:intergenic
GCTTTCCAAGGAGAAAGCAT+GGG 0.644753 4:-55385072 MsG0480021227.01.T01:CDS
CTTTCCCTGATGGCTCAAAG+TGG 0.648347 4:-55384797 MsG0480021227.01.T01:CDS
TATTGGTGAACCAGACCTCG+AGG 0.661264 4:+55385549 None:intergenic
CTCGATTAGTTCATACCACG+AGG 0.662449 4:-55385175 MsG0480021227.01.T01:CDS
GATGTTGAGTCAATGAGTGA+TGG 0.668644 4:-55385260 MsG0480021227.01.T01:CDS
ATTGGTGAACCAGACCTCGA+GGG 0.670776 4:+55385550 None:intergenic
TCTAGTTGTTGCCTTCGACG+AGG 0.686292 4:-55385501 MsG0480021227.01.T01:CDS
CAATGTTAAGGAGACCCTCG+AGG 0.715442 4:-55385564 MsG0480021227.01.T01:CDS
ATTGTAAACCCATATCCTCG+TGG 0.729649 4:+55385160 None:intergenic
TATCGGTGCAGGATACGTAG+GGG 0.733107 4:+55386950 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAATTTAATATT+TGG + Chr4:55384875-55384894 None:intergenic 0.0%
!!! TATTTTATTTTATTTTATTT+GGG - Chr4:55385846-55385865 MsG0480021227.01.T01:CDS 0.0%
!!! TTATTTTATTTTATTTTATT+TGG - Chr4:55385845-55385864 MsG0480021227.01.T01:CDS 0.0%
!! ATATTTGCATATTAAAAAAA+TGG - Chr4:55385295-55385314 MsG0480021227.01.T01:CDS 10.0%
!! TTGGAAAATAAATTGTAATA+AGG - Chr4:55385576-55385595 MsG0480021227.01.T01:CDS 15.0%
!!! ATGATATTTTAATTGGAAAA+GGG - Chr4:55385540-55385559 MsG0480021227.01.T01:CDS 15.0%
!! AAATTTAGTGCAACATATAA+AGG - Chr4:55385514-55385533 MsG0480021227.01.T01:CDS 20.0%
!! AATTTAGTGCAACATATAAA+GGG - Chr4:55385515-55385534 MsG0480021227.01.T01:CDS 20.0%
!!! AAAGGGAATGATATTTTAAT+TGG - Chr4:55385557-55385576 MsG0480021227.01.T01:CDS 20.0%
!!! ATAGGTAATTTATCTAGAAT+CGG + Chr4:55385493-55385512 None:intergenic 20.0%
!!! GATGATATTTTAATTGGAAA+AGG - Chr4:55385539-55385558 MsG0480021227.01.T01:CDS 20.0%
!!! TTATTTTATTTTATTTGGGC+AGG - Chr4:55385850-55385869 MsG0480021227.01.T01:CDS 20.0%
!!! TTGTTGATTATTATGTCAAT+GGG - Chr4:55386923-55386942 MsG0480021227.01.T01:intron 20.0%
!!! TTTCTCTTTCTAAGAAATTA+GGG - Chr4:55385095-55385114 MsG0480021227.01.T01:CDS 20.0%
! AAAGAGTTCTTCATTGAAAA+CGG + Chr4:55386722-55386741 None:intergenic 25.0%
! AAGTTGAAGTTAGAGAATTT+CGG + Chr4:55385625-55385644 None:intergenic 25.0%
! ACATTTCCTTTATCTTTCTT+TGG - Chr4:55385031-55385050 MsG0480021227.01.T01:CDS 25.0%
! ATTGATACTGTTGGAAAAAA+AGG - Chr4:55386346-55386365 MsG0480021227.01.T01:intron 25.0%
! CATGTTAAAATCAGAATTTG+TGG - Chr4:55385910-55385929 MsG0480021227.01.T01:intron 25.0%
! TAACCTCAGTAAATTAAAAC+CGG - Chr4:55386849-55386868 MsG0480021227.01.T01:intron 25.0%
! TAATTCACGTGAATAATTAC+CGG + Chr4:55386871-55386890 None:intergenic 25.0%
! TATGTTGCACTAAATTTGAT+AGG + Chr4:55385511-55385530 None:intergenic 25.0%
! TCAAAAATCAAGATACGATA+CGG + Chr4:55385373-55385392 None:intergenic 25.0%
! TCTAATATATTTGCCAATCT+CGG + Chr4:55386133-55386152 None:intergenic 25.0%
! TTTAATATTTGGATACTCTC+CGG + Chr4:55384864-55384883 None:intergenic 25.0%
!! CTTTCTCTTTCTAAGAAATT+AGG - Chr4:55385094-55385113 MsG0480021227.01.T01:CDS 25.0%
!! GTTGTTGATTATTATGTCAA+TGG - Chr4:55386922-55386941 MsG0480021227.01.T01:intron 25.0%
!!! AAGGGAGATGATATTTTAAT+TGG - Chr4:55385533-55385552 MsG0480021227.01.T01:CDS 25.0%
!!! ATCTTGATAATGATGAAGAA+AGG + Chr4:55385662-55385681 None:intergenic 25.0%
AAATGAACAGCTGAAAAAAC+AGG - Chr4:55385949-55385968 MsG0480021227.01.T01:intron 30.0%
AAGGCAACAACTAGATAATT+AGG + Chr4:55386256-55386275 None:intergenic 30.0%
AAGTCATTGGGAAAAAAGAA+AGG + Chr4:55385804-55385823 None:intergenic 30.0%
AATATTGACGATTAAGAGAG+TGG + Chr4:55385224-55385243 None:intergenic 30.0%
ATACTGTTGGAAAAAAAGGA+GGG - Chr4:55386350-55386369 MsG0480021227.01.T01:intron 30.0%
ATTAACAAACACAAGAAACC+AGG + Chr4:55385275-55385294 None:intergenic 30.0%
CATAATAACATGACAGCTTA+TGG - Chr4:55386526-55386545 MsG0480021227.01.T01:intron 30.0%
CATTATCAAGATTATGTGAG+AGG - Chr4:55385670-55385689 MsG0480021227.01.T01:CDS 30.0%
CTAATATATTTGCCAATCTC+GGG + Chr4:55386132-55386151 None:intergenic 30.0%
GAAAAAATGCTTCTTGCATT+AGG - Chr4:55386025-55386044 MsG0480021227.01.T01:intron 30.0%
TAACTCAATCAAAGTCGTAA+TGG - Chr4:55385697-55385716 MsG0480021227.01.T01:CDS 30.0%
TCGAAAATCAGAGTTCTTAT+AGG - Chr4:55385768-55385787 MsG0480021227.01.T01:CDS 30.0%
TGAGAAACAAAGAAGTCATT+GGG + Chr4:55385816-55385835 None:intergenic 30.0%
TTGAGAAACAAAGAAGTCAT+TGG + Chr4:55385817-55385836 None:intergenic 30.0%
! CTAAGAGAATTTTTGCAGTT+GGG - Chr4:55386406-55386425 MsG0480021227.01.T01:intron 30.0%
! TCATGCTTCTAAAAGAACTA+TGG - Chr4:55386771-55386790 MsG0480021227.01.T01:intron 30.0%
! TCTAAGAGAATTTTTGCAGT+TGG - Chr4:55386405-55386424 MsG0480021227.01.T01:intron 30.0%
!! AAGTTCTTTTCAAGACAGTA+AGG - Chr4:55386818-55386837 MsG0480021227.01.T01:intron 30.0%
!! TTCAACTTTTTCTTCCAAAG+TGG - Chr4:55385119-55385138 MsG0480021227.01.T01:CDS 30.0%
!!! AACTCTGATTTTCGAGTTAT+GGG + Chr4:55385763-55385782 None:intergenic 30.0%
!!! TTACCGGTTTTAATTTACTG+AGG + Chr4:55386855-55386874 None:intergenic 30.0%
ACAGTATCAATACCATCATC+CGG + Chr4:55386337-55386356 None:intergenic 35.0%
AGGATATGGGTTTACAATTC+TGG - Chr4:55386607-55386626 MsG0480021227.01.T01:intron 35.0%
ATTACCATCCTGATAAGCTT+CGG - Chr4:55386887-55386906 MsG0480021227.01.T01:intron 35.0%
CAAAAGCTCAATTGAAGGAA+TGG + Chr4:55386653-55386672 None:intergenic 35.0%
CTTAACATTGGAGTTTGCAA+GGG + Chr4:55386188-55386207 None:intergenic 35.0%
GATACTGTTGGAAAAAAAGG+AGG - Chr4:55386349-55386368 MsG0480021227.01.T01:intron 35.0%
TCACGGTATAGATGATCAAA+AGG + Chr4:55386478-55386497 None:intergenic 35.0%
TTAGGTAAACAGGAAAAAGC+TGG - Chr4:55386043-55386062 MsG0480021227.01.T01:intron 35.0%
TTCACCAATATCAAGAGAGT+CGG - Chr4:55386226-55386245 MsG0480021227.01.T01:intron 35.0%
TTGACTCAACATCTGAATCA+CGG + Chr4:55386495-55386514 None:intergenic 35.0%
! CATTCCTTCAATTGAGCTTT+TGG - Chr4:55386651-55386670 MsG0480021227.01.T01:intron 35.0%
! GGTAATTTATCTAGAATCGG+TGG + Chr4:55385490-55385509 None:intergenic 35.0%
!! AACAGTAGGATTGGTTCTTA+AGG + Chr4:55386089-55386108 None:intergenic 35.0%
!! GATGATGGTATTGATACTGT+TGG - Chr4:55386337-55386356 MsG0480021227.01.T01:intron 35.0%
!! TATACCGACTCTCTTGATAT+TGG + Chr4:55386233-55386252 None:intergenic 35.0%
!!! ACTCTGATTTTCGAGTTATG+GGG + Chr4:55385762-55385781 None:intergenic 35.0%
!!! GAACTCTGATTTTCGAGTTA+TGG + Chr4:55385764-55385783 None:intergenic 35.0%
!!! GTTATGGGGTTTTGAAGATT+TGG + Chr4:55385748-55385767 None:intergenic 35.0%
AATCAAGATACGATACGGCT+TGG + Chr4:55385368-55385387 None:intergenic 40.0%
AGCTCAATTGAAGGAATGGT+TGG + Chr4:55386649-55386668 None:intergenic 40.0%
AGTTCATACCACGAGGATAT+GGG - Chr4:55386594-55386613 MsG0480021227.01.T01:intron 40.0%
ATCTATGATGAACCCGTGAT+GGG - Chr4:55386559-55386578 MsG0480021227.01.T01:intron 40.0%
ATTGTAAACCCATATCCTCG+TGG + Chr4:55386605-55386624 None:intergenic 40.0%
CAGTATCAATACCATCATCC+GGG + Chr4:55386336-55386355 None:intergenic 40.0%
CCTTAACATTGGAGTTTGCA+AGG + Chr4:55386189-55386208 None:intergenic 40.0%
CCTTGCAAACTCCAATGTTA+AGG - Chr4:55386186-55386205 MsG0480021227.01.T01:intron 40.0%
CGATCCAAAAGCTCAATTGA+AGG + Chr4:55386658-55386677 None:intergenic 40.0%
CTGGCACCAAAGAAAGATAA+AGG + Chr4:55385040-55385059 None:intergenic 40.0%
GAAAGATGCAATCGTTTGTG+TGG + Chr4:55385079-55385098 None:intergenic 40.0%
GATGTTGAGTCAATGAGTGA+TGG - Chr4:55386502-55386521 MsG0480021227.01.T01:intron 40.0%
GCTTCTTGCATTAGGTAAAC+AGG - Chr4:55386033-55386052 MsG0480021227.01.T01:intron 40.0%
GTTCTTCATTGAAAACGGCT+TGG + Chr4:55386717-55386736 None:intergenic 40.0%
TAGTTCATACCACGAGGATA+TGG - Chr4:55386593-55386612 MsG0480021227.01.T01:intron 40.0%
TATCTATGATGAACCCGTGA+TGG - Chr4:55386558-55386577 MsG0480021227.01.T01:intron 40.0%
! ATTTTCCCGTATCAGCGTAT+CGG - Chr4:55385394-55385413 MsG0480021227.01.T01:CDS 40.0%
! CCCATTTTACAAAAGAGACC+CGG - Chr4:55386315-55386334 MsG0480021227.01.T01:intron 40.0%
! CCGGGTCTCTTTTGTAAAAT+GGG + Chr4:55386318-55386337 None:intergenic 40.0%
! TCCGGGTCTCTTTTGTAAAA+TGG + Chr4:55386319-55386338 None:intergenic 40.0%
!! CTTTGAAATTTCCTCGTCGA+AGG + Chr4:55386275-55386294 None:intergenic 40.0%
!!! CAAGAAGCATTTTTTCTGCC+TGG + Chr4:55386021-55386040 None:intergenic 40.0%
AAGGCCTTGACAGTACCAAA+AGG + Chr4:55386070-55386089 None:intergenic 45.0%
ATACTCTCCGGATACGTATC+GGG + Chr4:55384852-55384871 None:intergenic 45.0%
ATCAGGAGCAACAGTAGGAT+TGG + Chr4:55386098-55386117 None:intergenic 45.0%
ATGCAATCGTTTGTGTGGTG+TGG + Chr4:55385074-55385093 None:intergenic 45.0%
ATGTTAATGTGCTACGAGCC+TGG - Chr4:55385254-55385273 MsG0480021227.01.T01:CDS 45.0%
ATTTGCCAATCTCGGGTTCA+CGG + Chr4:55386125-55386144 None:intergenic 45.0%
GATACTCTCCGGATACGTAT+CGG + Chr4:55384853-55384872 None:intergenic 45.0%
GATACTTCCCGATACGTATC+CGG - Chr4:55384842-55384861 MsG0480021227.01.T01:CDS 45.0%
GATTCATCAGGAGCAACAGT+AGG + Chr4:55386103-55386122 None:intergenic 45.0%
GTCTGATACCAAGACTTCGA+CGG + Chr4:55385164-55385183 None:intergenic 45.0%
GTCTTGGTATCAGACGACTT+TGG - Chr4:55385169-55385188 MsG0480021227.01.T01:CDS 45.0%
TAATCGAGACCAACCCATCA+CGG + Chr4:55386575-55386594 None:intergenic 45.0%
TACAAAAGAGACCCGGATGA+TGG - Chr4:55386322-55386341 MsG0480021227.01.T01:intron 45.0%
TCCGTATCGTATCTCGTATC+CGG - Chr4:55385429-55385448 MsG0480021227.01.T01:CDS 45.0%
TTCATTCGCCGAAGCTTATC+AGG + Chr4:55386898-55386917 None:intergenic 45.0%
! AGGTGAGTGAGTTGACAGAA+AGG - Chr4:55385969-55385988 MsG0480021227.01.T01:intron 45.0%
! CAGGAAAAAGCTGGACCTTT+TGG - Chr4:55386052-55386071 MsG0480021227.01.T01:intron 45.0%
! CGTGACGTATCGGAAAGTAT+CGG + Chr4:55384912-55384931 None:intergenic 45.0%
! CTCGATTAGTTCATACCACG+AGG - Chr4:55386587-55386606 MsG0480021227.01.T01:intron 45.0%
! GCTTTCCAAGGAGAAAGCAT+GGG - Chr4:55386690-55386709 MsG0480021227.01.T01:intron 45.0%
! GGATACGTATCGGGAAGTAT+CGG + Chr4:55384843-55384862 None:intergenic 45.0%
! TGGACCTTTTGGTACTGTCA+AGG - Chr4:55386063-55386082 MsG0480021227.01.T01:intron 45.0%
!! GATACGTATCGGGAAGTATC+GGG + Chr4:55384842-55384861 None:intergenic 45.0%
!! GGAAAGGTTTGAGAGCATCT+TGG + Chr4:55386951-55386970 None:intergenic 45.0%
AAGCCCGTCATAACCAGGAT+GGG + Chr4:55386752-55386771 None:intergenic 50.0%
AATCGAGACCAACCCATCAC+GGG + Chr4:55386574-55386593 None:intergenic 50.0%
ATCGTTTGTGTGGTGTGGTG+TGG + Chr4:55385069-55385088 None:intergenic 50.0%
ATTGGTGAACCAGACCTCGA+GGG + Chr4:55386215-55386234 None:intergenic 50.0%
CAATGTTAAGGAGACCCTCG+AGG - Chr4:55386198-55386217 MsG0480021227.01.T01:intron 50.0%
CACTTTGAGCCATCAGGGAA+AGG + Chr4:55386967-55386986 None:intergenic 50.0%
CCCGGATACGAGATACGATA+CGG + Chr4:55385433-55385452 None:intergenic 50.0%
CCGATACGTCACGATACACA+CGG - Chr4:55384919-55384938 MsG0480021227.01.T01:CDS 50.0%
CCGTATCGTATCTCGTATCC+GGG - Chr4:55385430-55385449 MsG0480021227.01.T01:CDS 50.0%
CCGTGTGTATCGTGACGTAT+CGG + Chr4:55384922-55384941 None:intergenic 50.0%
CTCGAGGGTCTCCTTAACAT+TGG + Chr4:55386200-55386219 None:intergenic 50.0%
CTTTCCCTGATGGCTCAAAG+TGG - Chr4:55386965-55386984 MsG0480021227.01.T01:CDS 50.0%
GCTCTCAAACCTTTCCCTGA+TGG - Chr4:55386955-55386974 MsG0480021227.01.T01:CDS 50.0%
GGCTTTCCAAGGAGAAAGCA+TGG - Chr4:55386689-55386708 MsG0480021227.01.T01:intron 50.0%
TATCGGTGCAGGATACGTAG+GGG + Chr4:55384815-55384834 None:intergenic 50.0%
TATTGGTGAACCAGACCTCG+AGG + Chr4:55386216-55386235 None:intergenic 50.0%
TCGTTTGTGTGGTGTGGTGT+GGG + Chr4:55385068-55385087 None:intergenic 50.0%
TCTAGTTGTTGCCTTCGACG+AGG - Chr4:55386261-55386280 MsG0480021227.01.T01:intron 50.0%
TGAATCCGTGAACCCGAGAT+TGG - Chr4:55386117-55386136 MsG0480021227.01.T01:intron 50.0%
TGATACCAAGACTTCGACGG+TGG + Chr4:55385161-55385180 None:intergenic 50.0%
TGCAGAGCAAGGAAAAGACC+AGG - Chr4:55386000-55386019 MsG0480021227.01.T01:intron 50.0%
TGCAGGATACGTAGGGGATA+CGG + Chr4:55384809-55384828 None:intergenic 50.0%
TTCGCCGAAGCTTATCAGGA+TGG + Chr4:55386894-55386913 None:intergenic 50.0%
TTGGTCCCATGCTTTCTCCT+TGG + Chr4:55386698-55386717 None:intergenic 50.0%
! ACTGCACCGATACGCTGATA+CGG + Chr4:55385403-55385422 None:intergenic 50.0%
! CTCTTTTATCCGTCCCATCC+TGG - Chr4:55386736-55386755 MsG0480021227.01.T01:intron 50.0%
! GTATCGGTGCAGGATACGTA+GGG + Chr4:55384816-55384835 None:intergenic 50.0%
! TAGTGTGTTGCTGTGAAGCC+CGG + Chr4:55385451-55385470 None:intergenic 50.0%
! TTTTGGATCGTGTCGCGACA+AGG - Chr4:55386668-55386687 MsG0480021227.01.T01:intron 50.0%
!! ATGATGAACCCGTGATGGGT+TGG - Chr4:55386563-55386582 MsG0480021227.01.T01:intron 50.0%
AAAGTGGACGGCGACCACTT+TGG + Chr4:55385136-55385155 None:intergenic 55.0%
AAAGTGGTCGCCGTCCACTT+TGG - Chr4:55385135-55385154 MsG0480021227.01.T01:CDS 55.0%
AGGCTTCAGCTTGCAGAGCA+AGG - Chr4:55385989-55386008 MsG0480021227.01.T01:intron 55.0%
CCGTCATAACCAGGATGGGA+CGG + Chr4:55386748-55386767 None:intergenic 55.0%
CCGTCCCATCCTGGTTATGA+CGG - Chr4:55386745-55386764 MsG0480021227.01.T01:intron 55.0%
CGTCCCATCCTGGTTATGAC+GGG - Chr4:55386746-55386765 MsG0480021227.01.T01:intron 55.0%
CTCGGGTTCACGGATTCATC+AGG + Chr4:55386115-55386134 None:intergenic 55.0%
GAAGCCCGTCATAACCAGGA+TGG + Chr4:55386753-55386772 None:intergenic 55.0%
GCATGAAGCCCGTCATAACC+AGG + Chr4:55386757-55386776 None:intergenic 55.0%
TGTCGCGACAAGGCTTTCCA+AGG - Chr4:55386678-55386697 MsG0480021227.01.T01:intron 55.0%
TTAAGGAGACCCTCGAGGTC+TGG - Chr4:55386203-55386222 MsG0480021227.01.T01:intron 55.0%
TTTGGCCACCGTCGAAGTCT+TGG - Chr4:55385153-55385172 MsG0480021227.01.T01:CDS 55.0%
! CTGCACCGATACGCTGATAC+GGG + Chr4:55385402-55385421 None:intergenic 55.0%
!! GGGAAGTATCGGGCAGGTAT+CGG + Chr4:55384832-55384851 None:intergenic 55.0%
!! GGTATCGGTGCAGGATACGT+AGG + Chr4:55384817-55384836 None:intergenic 55.0%
GACTTCGACGGTGGCCAAAG+TGG + Chr4:55385152-55385171 None:intergenic 60.0%
GAGGGAGAGCAGCTGTCTCT+GGG - Chr4:55386368-55386387 MsG0480021227.01.T01:intron 60.0%
TCGACGGTGGCCAAAGTGGA+CGG + Chr4:55385148-55385167 None:intergenic 60.0%
TGGTGTGGTGTGGGTCGTAC+TGG + Chr4:55385059-55385078 None:intergenic 60.0%
!! CGTATCGGGAAGTATCGGGC+AGG + Chr4:55384838-55384857 None:intergenic 60.0%
!! TATCGGGCAGGTATCGGTGC+AGG + Chr4:55384826-55384845 None:intergenic 60.0%
GGAGGGAGAGCAGCTGTCTC+TGG - Chr4:55386367-55386386 MsG0480021227.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 55384794 55386990 55384794 ID=MsG0480021227.01;Name=MsG0480021227.01
Chr4 mRNA 55384794 55386990 55384794 ID=MsG0480021227.01.T01;Parent=MsG0480021227.01;Name=MsG0480021227.01.T01;_AED=0.49;_eAED=0.52;_QI=0|0|0|1|1|1|2|0|393
Chr4 exon 55386928 55386990 55386928 ID=MsG0480021227.01.T01:exon:18936;Parent=MsG0480021227.01.T01
Chr4 exon 55384794 55385912 55384794 ID=MsG0480021227.01.T01:exon:18935;Parent=MsG0480021227.01.T01
Chr4 CDS 55386928 55386990 55386928 ID=MsG0480021227.01.T01:cds;Parent=MsG0480021227.01.T01
Chr4 CDS 55384794 55385912 55384794 ID=MsG0480021227.01.T01:cds;Parent=MsG0480021227.01.T01
Gene Sequence

>MsG0480021227.01.T01

ATGGATGACGTACCGTATCCCCTACGTATCCTGCACCGATACCTGCCCGATACTTCCCGATACGAAAATTCAGCTGAAAGTGAATCGTCAGAAAGAGTTAACATGTTAAAATCAGAATTTGTGGCACTCTTATCAGATAAAAATGAACAGCTGAAAAAACAGGTGAGTGAGTTGACAGAAAGGCTTCAGCTTGCAGAGCAAGGAAAAGACCAGGCAGAAAAAATGCTTCTTGCATTAGGTAAACAGGAAAAAGCTGGACCTTTTGGTACTGTCAAGGCCTTAAGAACCAATCCTACTGTTGCTCCTGATGAATCCGTGAACCCGAGATTGGCAAATATATTAGATAAAATCGCAGTTAAACGAGAGATCATAGTTGCCCTTGCAAACTCCAATGTTAAGGAGACCCTCGAGGTCTGGTTCACCAATATCAAGAGAGTCGGTATACCTAATTATCTAGTTGTTGCCTTCGACGAGGAAATTTCAAAGTTTTGCGAATCGAATAAAGTCCCATTTTACAAAAGAGACCCGGATGATGGTATTGATACTGTTGGAAAAAAAGGAGGGAGAGCAGCTGTCTCTGGGCTTAAATTTCGTGTTCTAAGAGAATTTTTGCAGTTGGGATATAGTGTTCTTCTATCAGACATCGACATTATATATTTGCAGAATCCTTTTGATCATCTATACCGTGATTCAGATGTTGAGTCAATGAGTGATGGTCATAATAACATGACAGCTTATGGCTACAACGATATCTATGATGAACCCGTGATGGGTTGGTCTCGATTAGTTCATACCACGAGGATATGGGTTTACAATTCTGGTTTCTTCTATATTAGACCAACCATTCCTTCAATTGAGCTTTTGGATCGTGTCGCGACAAGGCTTTCCAAGGAGAAAGCATGGGACCAAGCCGTTTTCAATGAAGAACTCTTTTATCCGTCCCATCCTGGTTATGACGGGCTTCATGCTTCTAAAAGAACTATGGATATGTATCTTTTTATGAACAGCAAAGTTCTTTTCAAGACAGTAAGGAATGATGCTAACCTCAGTAAATTAAAACCGGTAATTATTCACGTGAATTACCATCCTGATAAGCTTCGGCGAATGAAAGCAGTTGTTGATTATTATGTCAATGGGAACCAAGATGCTCTCAAACCTTTCCCTGATGGCTCAAAGTGGTAA
Protein sequence

>MsG0480021227.01.T01

MDDVPYPLRILHRYLPDTSRYENSAESESSERVNMLKSEFVALLSDKNEQLKKQVSELTERLQLAEQGKDQAEKMLLALGKQEKAGPFGTVKALRTNPTVAPDESVNPRLANILDKIAVKREIIVALANSNVKETLEVWFTNIKRVGIPNYLVVAFDEEISKFCESNKVPFYKRDPDDGIDTVGKKGGRAAVSGLKFRVLREFLQLGYSVLLSDIDIIYLQNPFDHLYRDSDVESMSDGHNNMTAYGYNDIYDEPVMGWSRLVHTTRIWVYNSGFFYIRPTIPSIELLDRVATRLSKEKAWDQAVFNEELFYPSHPGYDGLHASKRTMDMYLFMNSKVLFKTVRNDANLSKLKPVIIHVNYHPDKLRRMKAVVDYYVNGNQDALKPFPDGSKW*