AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045775.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045775.01.T01 MTR_8g078760 85.758 330 22 1 8 312 43 372 0.0 569
MsG0880045775.01.T01 MTR_8g078760 85.758 330 22 1 8 312 43 372 0.0 569
MsG0880045775.01.T01 MTR_8g078760 87.755 98 10 2 315 411 530 626 1.19e-47 172
MsG0880045775.01.T01 MTR_5g018820 56.495 331 106 8 8 312 51 369 7.82e-99 307
MsG0880045775.01.T01 MTR_5g018820 65.517 87 28 2 326 411 539 624 2.60e-28 117
MsG0880045775.01.T01 MTR_4g068920 30.878 353 159 13 8 312 64 379 1.32e-25 109
MsG0880045775.01.T01 MTR_4g068920 51.765 85 39 2 327 410 550 633 2.74e-16 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 14 320 2.55e-68 223
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 68 374 4.26e-68 224
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 68 374 4.26e-68 224
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 68 374 4.26e-68 224
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 68 374 1.62e-67 223
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 68 374 1.62e-67 223
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 68 374 1.62e-67 223
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 68 374 1.62e-67 223
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 14 320 1.18e-66 222
MsG0880045775.01.T01 AT5G66600 54.455 101 40 4 315 411 462 560 1.98e-23 102
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 48 354 1.61e-66 222
MsG0880045775.01.T01 AT5G66600 54.455 101 40 4 315 411 496 594 2.03e-23 102
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 68 374 1.61e-66 223
MsG0880045775.01.T01 AT5G66600 54.455 101 40 4 315 411 516 614 2.06e-23 103
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 68 374 1.61e-66 223
MsG0880045775.01.T01 AT5G66600 54.455 101 40 4 315 411 516 614 2.06e-23 103
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 68 374 1.61e-66 223
MsG0880045775.01.T01 AT5G66600 54.455 101 40 4 315 411 516 614 2.06e-23 103
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 68 374 1.61e-66 223
MsG0880045775.01.T01 AT5G66600 54.455 101 40 4 315 411 516 614 2.06e-23 103
MsG0880045775.01.T01 AT5G66600 46.130 323 128 8 26 318 68 374 1.61e-66 223
MsG0880045775.01.T01 AT5G66600 54.455 101 40 4 315 411 516 614 2.06e-23 103
MsG0880045775.01.T01 AT2G23700 64.368 87 28 3 327 411 622 707 4.49e-28 117
MsG0880045775.01.T01 AT2G23700 52.459 122 54 2 19 140 58 175 1.25e-24 107
MsG0880045775.01.T01 AT2G23700 41.477 176 78 4 146 300 280 451 5.29e-22 99.0
MsG0880045775.01.T01 AT1G76620 42.453 106 58 3 307 410 417 521 7.21e-16 79.7
MsG0880045775.01.T01 AT3G18900 42.268 97 52 4 312 405 354 449 1.61e-14 75.5
MsG0880045775.01.T01 AT3G18900 42.268 97 52 4 312 405 354 449 1.61e-14 75.5
MsG0880045775.01.T01 AT3G18900 42.268 97 52 4 312 405 430 525 2.07e-14 75.5
MsG0880045775.01.T01 AT3G18900 42.268 97 52 4 312 405 435 530 2.09e-14 75.5
MsG0880045775.01.T01 AT5G47380 27.241 290 166 8 12 286 17 276 1.28e-13 72.8
MsG0880045775.01.T01 AT5G47380 27.402 281 160 7 21 286 81 332 1.28e-13 72.8
MsG0880045775.01.T01 AT5G47380 27.402 281 160 7 21 286 81 332 1.31e-13 73.2
MsG0880045775.01.T01 AT5G47380 27.402 281 160 7 21 286 81 332 1.34e-13 73.2
MsG0880045775.01.T01 AT5G47380 27.402 281 160 7 21 286 81 332 1.43e-13 72.8
MsG0880045775.01.T01 AT1G21060 40.000 95 55 2 315 408 401 494 3.72e-12 68.2
MsG0880045775.01.T01 AT1G21060 40.000 95 55 2 315 408 413 506 4.12e-12 68.2

Find 93 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGCTTATTAGCTGTTTC+TGG 0.133513 8:-64241731 None:intergenic
AGAGTTAGAAGTTGTATATT+TGG 0.184488 8:+64241044 MsG0880045775.01.T01:CDS
CCTCGGGTGGAGAAACTTTA+AGG 0.205914 8:-64241194 None:intergenic
CTGGAATATGATCACATATT+CGG 0.260667 8:-64241712 None:intergenic
TGTACAATGTATTCTTTATC+TGG 0.269728 8:-64241309 None:intergenic
ACATTTGTCTCCAAAGATTC+AGG 0.273292 8:+64243255 MsG0880045775.01.T01:CDS
ACACTCTTTCTCAGGGATTC+AGG 0.278846 8:-64243321 None:intergenic
TTTGCTGTAGATGTTGATAT+TGG 0.282756 8:-64241389 None:intergenic
CTTGCTCTAGGTGTTGTTAC+AGG 0.300352 8:-64241168 None:intergenic
AAGACTAGAAGACCAATTTA+AGG 0.302009 8:+64240524 MsG0880045775.01.T01:CDS
TATGAATTTGATCTGGTTCC+AGG 0.307818 8:+64240276 None:intergenic
TAATAAGCTTTCTGAGGATA+TGG 0.308473 8:+64241742 MsG0880045775.01.T01:CDS
ACAAATGTATCTACAATCTT+AGG 0.318285 8:-64243240 None:intergenic
ATTTGGATTAACAAGAGATA+TGG 0.335609 8:-64240583 None:intergenic
TGGGGTATTGTCGTGTCATT+TGG 0.343001 8:-64240600 None:intergenic
GTTGTTGTTCAAATGCTTTA+CGG 0.343548 8:-64241089 None:intergenic
ATTTCTTAACACTCTTTCTC+AGG 0.352274 8:-64243329 None:intergenic
ATGAGACAAACCTGAATCTT+TGG 0.370103 8:-64243265 None:intergenic
AAGAAATGTCATGCAAAATC+CGG 0.384303 8:+64243345 MsG0880045775.01.T01:CDS
AATCCATTCTATGCTCTTCC+CGG 0.388268 8:-64243364 None:intergenic
GATAATCCTTTCAACGTTGA+AGG 0.391580 8:+64241926 MsG0880045775.01.T01:CDS
GAAAGGCCCGGATTAGTTAT+AGG 0.396919 8:-64241806 None:intergenic
AAAGGCACTTATTGAAGTAC+TGG 0.399956 8:-64241834 None:intergenic
TTGTCTACTGTAACAGATCC+TGG 0.402270 8:+64243722 MsG0880045775.01.T01:three_prime_UTR
TATTCTTTATCTGGAGTCTC+AGG 0.406473 8:-64241300 None:intergenic
TGGAATATGATCACATATTC+GGG 0.406975 8:-64241711 None:intergenic
ATGTGCATCGGACCTTAAAT+TGG 0.409450 8:-64240536 None:intergenic
AGTTCTTGAAACACTCTCTT+CGG 0.410304 8:-64243151 None:intergenic
GTATGTCGAACATTATTCAT+AGG 0.412449 8:-64240346 None:intergenic
TTCAAATGCTTTACGGTACA+AGG 0.416852 8:-64241082 None:intergenic
AATTATTTAACCAGCTCCTT+AGG 0.423672 8:-64243417 None:intergenic
TTGCTGTAGATGTTGATATT+GGG 0.430253 8:-64241388 None:intergenic
CAAGGCGACACGTGGAGTCC+AGG 0.433939 8:+64241869 MsG0880045775.01.T01:CDS
GTAGTCAATCAAATGCCAAT+TGG 0.457286 8:+64243861 MsG0880045775.01.T01:three_prime_UTR
TTTGGATTAACAAGAGATAT+GGG 0.457433 8:-64240582 None:intergenic
GAGTACATTAGAGCCACGTT+TGG 0.459510 8:+64243189 MsG0880045775.01.T01:CDS
AGACCCACCTATAACTAATC+CGG 0.459828 8:+64241799 MsG0880045775.01.T01:CDS
TCTTTGATTAATTCTGTGGT+TGG 0.461028 8:-64241009 None:intergenic
TGAGATTCATACACTTCAAA+AGG 0.462070 8:+64241260 MsG0880045775.01.T01:CDS
TTTCAACGTTGAAGGATTCA+AGG 0.464834 8:+64241934 MsG0880045775.01.T01:CDS
TTGAATCCTTCAACGTTGAA+AGG 0.468691 8:-64241932 None:intergenic
AATGTCTTTGATTAATTCTG+TGG 0.470476 8:-64241013 None:intergenic
CCTAAAGTATGAATTTGATC+TGG 0.471195 8:+64240269 None:intergenic
TATGTCGAACATTATTCATA+GGG 0.472023 8:-64240345 None:intergenic
AGTACATTAGAGCCACGTTT+GGG 0.481357 8:+64243190 MsG0880045775.01.T01:CDS
GAAGTTCAAAGAAATCCAAT+TGG 0.484909 8:-64243876 None:intergenic
ACTGTCTGGTCACATATTAA+CGG 0.488449 8:+64243667 MsG0880045775.01.T01:three_prime_UTR
TTCTGCTAGACTGATTACTT+TGG 0.492321 8:-64241678 None:intergenic
ACACTTCAAAAGGAATGTAA+TGG 0.493365 8:+64241270 MsG0880045775.01.T01:CDS
CTGTGAGGTAACCTGTGTCC+TGG 0.499319 8:-64240294 None:intergenic
GAGCTGGTTAAATAATTACT+TGG 0.505790 8:+64243423 MsG0880045775.01.T01:exon
ATACAGTATGCTGAAAGTGT+TGG 0.509241 8:+64241649 MsG0880045775.01.T01:intron
GATGCAGATGCAGCCACAGC+GGG 0.513708 8:-64241117 None:intergenic
GCCTTGATCGCCAATAGAAA+AGG 0.516651 8:-64241852 None:intergenic
ATCTAACTGTACATCAAAAG+AGG 0.517517 8:-64241906 None:intergenic
GAGGAGTTATTTCTTAACCC+TGG 0.518333 8:-64241887 None:intergenic
CTCAACTGTTAATTTGAGAT+CGG 0.519091 8:-64243602 None:intergenic
GGGATTCAGGTAGAGACTGT+TGG 0.520236 8:-64243308 None:intergenic
GATTCAGGTTTGTCTCATGC+TGG 0.527367 8:+64243270 MsG0880045775.01.T01:CDS
TGAGACCATCTGCAAATGTT+TGG 0.535765 8:+64243456 MsG0880045775.01.T01:three_prime_UTR
TTCAAGAACTAGAATTGCAA+AGG 0.553396 8:+64243164 MsG0880045775.01.T01:CDS
AGAAATGTCATGCAAAATCC+GGG 0.554408 8:+64243346 MsG0880045775.01.T01:CDS
ACTTTAAGGAATCTTGCTCT+AGG 0.557002 8:-64241180 None:intergenic
TTGATCTGGTTCCAGGACAC+AGG 0.559997 8:+64240283 MsG0880045775.01.T01:CDS
ATATCTGATACCTAAGGAGC+TGG 0.567502 8:+64243407 MsG0880045775.01.T01:CDS
AATCCGGGAAGAGCATAGAA+TGG 0.571165 8:+64243361 MsG0880045775.01.T01:CDS
TTGAAGTACTGGGAGATGAA+AGG 0.575634 8:-64241823 None:intergenic
ATTGACCAAACATTTGCAGA+TGG 0.581951 8:-64243461 None:intergenic
TTTCTTAACACTCTTTCTCA+GGG 0.582135 8:-64243328 None:intergenic
TATCGAAAAGTGAAGTTGTG+TGG 0.588149 8:-64243387 None:intergenic
GACCCACCTATAACTAATCC+GGG 0.590808 8:+64241800 MsG0880045775.01.T01:CDS
TCACAGAATGCAGTGCAACT+AGG 0.592322 8:+64240311 MsG0880045775.01.T01:CDS
ATTGTGAAATCTGAACTGTC+TGG 0.593513 8:+64243653 MsG0880045775.01.T01:three_prime_UTR
AAATGACACGACAATACCCC+AGG 0.596729 8:+64240602 MsG0880045775.01.T01:CDS
AAGGCGACACGTGGAGTCCA+GGG 0.597096 8:+64241870 MsG0880045775.01.T01:CDS
GCTCTAGGTGTTGTTACAGG+AGG 0.608021 8:-64241165 None:intergenic
CCTTAAAGTTTCTCCACCCG+AGG 0.610761 8:+64241194 MsG0880045775.01.T01:CDS
AACAGCTAATAAGCTTTCTG+AGG 0.613973 8:+64241736 MsG0880045775.01.T01:CDS
AAGGCACTTATTGAAGTACT+GGG 0.618965 8:-64241833 None:intergenic
TTGGCGATCAAGGCGACACG+TGG 0.623203 8:+64241861 MsG0880045775.01.T01:CDS
GTACTGGGAGATGAAAGGCC+CGG 0.626536 8:-64241818 None:intergenic
GGCCCGGATTAGTTATAGGT+GGG 0.627934 8:-64241802 None:intergenic
AGGCCCGGATTAGTTATAGG+TGG 0.635235 8:-64241803 None:intergenic
ATCAGTCTAGCAGAACATCT+CGG 0.636239 8:+64241686 MsG0880045775.01.T01:CDS
TCGACATACGCTGAAGCAAG+AGG 0.637847 8:+64240360 MsG0880045775.01.T01:CDS
ATGTCGAACATTATTCATAG+GGG 0.640528 8:-64240344 None:intergenic
GGCTGCATCTGCATCTACCG+TGG 0.641968 8:+64241125 MsG0880045775.01.T01:CDS
GTCCTTGCGTATCCCAAACG+TGG 0.649954 8:-64243202 None:intergenic
AGCCACGTTTGGGATACGCA+AGG 0.661192 8:+64243200 MsG0880045775.01.T01:CDS
ATGTTGATATTGGGATAACG+AGG 0.672943 8:-64241379 None:intergenic
TAGTTGCACTGCATTCTGTG+AGG 0.692782 8:-64240309 None:intergenic
AGATGCAGATGCAGCCACAG+CGG 0.716343 8:-64241118 None:intergenic
ACAACAACTATCTCCCGCTG+TGG 0.724825 8:+64241104 MsG0880045775.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATATGATTAAAACAAAA+TGG - Chr8:64240448-64240467 None:intergenic 10.0%
!! ACAATTTAGTATATTAATTC+TGG - Chr8:64240782-64240801 None:intergenic 15.0%
!!! ATTGTTTTTTGAAATAACAT+TGG + Chr8:64240797-64240816 MsG0880045775.01.T01:intron 15.0%
!!! TGTTATAAGTTTGAAATATT+TGG + Chr8:64240922-64240941 MsG0880045775.01.T01:intron 15.0%
!! ACAATACAAAATACTATACT+AGG + Chr8:64242431-64242450 MsG0880045775.01.T01:intron 20.0%
!! AGAGAAATGAAATGTATTAA+AGG - Chr8:64242185-64242204 None:intergenic 20.0%
!! CAATACAAAATACTATACTA+GGG + Chr8:64242432-64242451 MsG0880045775.01.T01:intron 20.0%
!! CATATAAATAATTACACACA+AGG - Chr8:64242555-64242574 None:intergenic 20.0%
!! GAGAAATGAAATGTATTAAA+GGG - Chr8:64242184-64242203 None:intergenic 20.0%
!! TTGAATCATGAATCAAAAAT+AGG - Chr8:64243944-64243963 None:intergenic 20.0%
!!! ACAATAGGAATTTTAACTAA+GGG - Chr8:64242725-64242744 None:intergenic 20.0%
!!! CAATTATTGTGTATTCTTTT+CGG + Chr8:64243480-64243499 MsG0880045775.01.T01:three_prime_UTR 20.0%
!!! GCCAAATTTTAATCAAATTA+AGG - Chr8:64241617-64241636 None:intergenic 20.0%
!!! GCCTTAATTTGATTAAAATT+TGG + Chr8:64241613-64241632 MsG0880045775.01.T01:intron 20.0%
! AAATGTATTAAAGGGTAACA+TGG - Chr8:64242176-64242195 None:intergenic 25.0%
! AATGTCTTTGATTAATTCTG+TGG - Chr8:64241016-64241035 None:intergenic 25.0%
! ACAAATGTATCTACAATCTT+AGG - Chr8:64243243-64243262 None:intergenic 25.0%
! AGAGTTAGAAGTTGTATATT+TGG + Chr8:64241044-64241063 MsG0880045775.01.T01:CDS 25.0%
! AGCATCAGGTATTATAAAAA+TGG - Chr8:64242976-64242995 None:intergenic 25.0%
! ATGATCATTACACATTGTTT+AGG + Chr8:64240895-64240914 MsG0880045775.01.T01:intron 25.0%
! ATTTCTCTCACATGATTAAT+TGG + Chr8:64242197-64242216 MsG0880045775.01.T01:intron 25.0%
! ATTTGGATTAACAAGAGATA+TGG - Chr8:64240586-64240605 None:intergenic 25.0%
! CATATTAACTTTATGTTTGC+CGG - Chr8:64240866-64240885 None:intergenic 25.0%
! TATGTCGAACATTATTCATA+GGG - Chr8:64240348-64240367 None:intergenic 25.0%
! TGATTCTCTTTATAAATCCA+CGG - Chr8:64241995-64242014 None:intergenic 25.0%
! TGTACAATGTATTCTTTATC+TGG - Chr8:64241312-64241331 None:intergenic 25.0%
! TTATAAAGAGAATCAGAAGT+TGG + Chr8:64242000-64242019 MsG0880045775.01.T01:intron 25.0%
! TTTGGATTAACAAGAGATAT+GGG - Chr8:64240585-64240604 None:intergenic 25.0%
!! ACAATCTGTTAATTTGAGAT+CGG - Chr8:64243556-64243575 None:intergenic 25.0%
!!! AATCTTATTGATAGTGAACT+TGG - Chr8:64240692-64240711 None:intergenic 25.0%
!!! AGTTTTAACCAATTATCAAC+TGG + Chr8:64240744-64240763 MsG0880045775.01.T01:intron 25.0%
!!! CACAATAGGAATTTTAACTA+AGG - Chr8:64242726-64242745 None:intergenic 25.0%
AAGAAATGTCATGCAAAATC+CGG + Chr8:64243345-64243364 MsG0880045775.01.T01:CDS 30.0%
AAGACTAGAAGACCAATTTA+AGG + Chr8:64240524-64240543 MsG0880045775.01.T01:CDS 30.0%
AATTATTTAACCAGCTCCTT+AGG - Chr8:64243420-64243439 None:intergenic 30.0%
AATTCTTATAGGGTTAGAGT+TGG - Chr8:64242497-64242516 None:intergenic 30.0%
ACAAGCGGTTAATTCTTATA+GGG - Chr8:64242507-64242526 None:intergenic 30.0%
ACACTTCAAAAGGAATGTAA+TGG + Chr8:64241270-64241289 MsG0880045775.01.T01:CDS 30.0%
ATCTAACTGTACATCAAAAG+AGG - Chr8:64241909-64241928 None:intergenic 30.0%
ATGTCGAACATTATTCATAG+GGG - Chr8:64240347-64240366 None:intergenic 30.0%
ATTCTTATAGGGTTAGAGTT+GGG - Chr8:64242496-64242515 None:intergenic 30.0%
ATTTCTTAACACTCTTTCTC+AGG - Chr8:64243332-64243351 None:intergenic 30.0%
CAAAAAAGCCTATTAGAAGA+AGG - Chr8:64242229-64242248 None:intergenic 30.0%
CATAACAGAACTGATGAATT+AGG - Chr8:64241542-64241561 None:intergenic 30.0%
CTGGAATATGATCACATATT+CGG - Chr8:64241715-64241734 None:intergenic 30.0%
GAACCATACTGCAAAAAAAA+TGG - Chr8:64240419-64240438 None:intergenic 30.0%
GAAGTTCAAAGAAATCCAAT+TGG - Chr8:64243879-64243898 None:intergenic 30.0%
GAGCTGGTTAAATAATTACT+TGG + Chr8:64243423-64243442 MsG0880045775.01.T01:exon 30.0%
GGTTTATTCCTTCTTCTAAT+AGG + Chr8:64242218-64242237 MsG0880045775.01.T01:intron 30.0%
GTATGTCGAACATTATTCAT+AGG - Chr8:64240349-64240368 None:intergenic 30.0%
TAAACGCAATATGATACAAG+CGG - Chr8:64242522-64242541 None:intergenic 30.0%
TACAAGCGGTTAATTCTTAT+AGG - Chr8:64242508-64242527 None:intergenic 30.0%
TCTTTGATTAATTCTGTGGT+TGG - Chr8:64241012-64241031 None:intergenic 30.0%
TGAGATTCATACACTTCAAA+AGG + Chr8:64241260-64241279 MsG0880045775.01.T01:CDS 30.0%
TGGAATATGATCACATATTC+GGG - Chr8:64241714-64241733 None:intergenic 30.0%
TTCAAGAACTAGAATTGCAA+AGG + Chr8:64243164-64243183 MsG0880045775.01.T01:CDS 30.0%
TTCAGTACTCAACATTTGTT+TGG + Chr8:64243005-64243024 MsG0880045775.01.T01:intron 30.0%
TTCGAAGAAAAATTCAAAGC+TGG + Chr8:64242810-64242829 MsG0880045775.01.T01:intron 30.0%
TTGCTGTAGATGTTGATATT+GGG - Chr8:64241391-64241410 None:intergenic 30.0%
TTTCTTAACACTCTTTCTCA+GGG - Chr8:64243331-64243350 None:intergenic 30.0%
! ATTCTTTTCGGATTTGGATT+TGG + Chr8:64243492-64243511 MsG0880045775.01.T01:three_prime_UTR 30.0%
! CTCAACTGTTAATTTGAGAT+CGG - Chr8:64243605-64243624 None:intergenic 30.0%
! GAGAATGATAGTACTTTTCT+AGG + Chr8:64241591-64241610 MsG0880045775.01.T01:intron 30.0%
! GTTGTTGTTCAAATGCTTTA+CGG - Chr8:64241092-64241111 None:intergenic 30.0%
! TAATAAGCTTTCTGAGGATA+TGG + Chr8:64241742-64241761 MsG0880045775.01.T01:CDS 30.0%
! TCAATAAGTGCCTTTTCTAT+TGG + Chr8:64241842-64241861 MsG0880045775.01.T01:CDS 30.0%
! TGTCAATTTTGTACAAAACC+AGG - Chr8:64243743-64243762 None:intergenic 30.0%
! TTGTGTATTCTTTTCGGATT+TGG + Chr8:64243486-64243505 MsG0880045775.01.T01:three_prime_UTR 30.0%
! TTTGCTGTAGATGTTGATAT+TGG - Chr8:64241392-64241411 None:intergenic 30.0%
! TTTTCGATATCTGATACCTA+AGG + Chr8:64243401-64243420 MsG0880045775.01.T01:CDS 30.0%
!! ACTCTTTTCACATTGTTTTG+TGG - Chr8:64242264-64242283 None:intergenic 30.0%
!! GGTTTTGTACAAAATTGACA+AGG + Chr8:64243743-64243762 MsG0880045775.01.T01:three_prime_UTR 30.0%
!!! CTTCTTCTAATAGGCTTTTT+TGG + Chr8:64242227-64242246 MsG0880045775.01.T01:intron 30.0%
!!! TAATAGGCTTTTTTGGCTTA+TGG + Chr8:64242234-64242253 MsG0880045775.01.T01:intron 30.0%
!!! TACATCTGAACATCCTTTTT+TGG - Chr8:64241226-64241245 None:intergenic 30.0%
!!! TCTTAGGTAGAAGTATTTTC+TGG - Chr8:64243227-64243246 None:intergenic 30.0%
AACAGCTAATAAGCTTTCTG+AGG + Chr8:64241736-64241755 MsG0880045775.01.T01:CDS 35.0%
AAGAGTCTTTCTTCTCTTGA+AGG + Chr8:64242278-64242297 MsG0880045775.01.T01:intron 35.0%
AAGGATTCAAGGAGTTTAGT+GGG + Chr8:64241945-64241964 MsG0880045775.01.T01:intron 35.0%
AATTAGAAGACGTAGATCCA+GGG + Chr8:64242067-64242086 MsG0880045775.01.T01:intron 35.0%
ACATTTGTCTCCAAAGATTC+AGG + Chr8:64243255-64243274 MsG0880045775.01.T01:CDS 35.0%
ACCAAAACTACATCTATGCA+TGG - Chr8:64240827-64240846 None:intergenic 35.0%
ACTGTCTGGTCACATATTAA+CGG + Chr8:64243667-64243686 MsG0880045775.01.T01:three_prime_UTR 35.0%
AGAAATGTCATGCAAAATCC+GGG + Chr8:64243346-64243365 MsG0880045775.01.T01:CDS 35.0%
AGTTCTTGAAACACTCTCTT+CGG - Chr8:64243154-64243173 None:intergenic 35.0%
ATGAGACAAACCTGAATCTT+TGG - Chr8:64243268-64243287 None:intergenic 35.0%
ATGTTGATATTGGGATAACG+AGG - Chr8:64241382-64241401 None:intergenic 35.0%
ATTGAAGAAATTCCAACCTG+GGG - Chr8:64240621-64240640 None:intergenic 35.0%
ATTGACCAAACATTTGCAGA+TGG - Chr8:64243464-64243483 None:intergenic 35.0%
ATTGTGAAATCTGAACTGTC+TGG + Chr8:64243653-64243672 MsG0880045775.01.T01:three_prime_UTR 35.0%
CAATAAGTGACCTGAAATTC+TGG - Chr8:64242045-64242064 None:intergenic 35.0%
CATTGAAGAAATTCCAACCT+GGG - Chr8:64240622-64240641 None:intergenic 35.0%
CTGAATGTAAAGATAGCATC+AGG - Chr8:64242990-64243009 None:intergenic 35.0%
GAAAAATTCAAAGCTGGAGA+TGG + Chr8:64242816-64242835 MsG0880045775.01.T01:intron 35.0%
GAAAGTCAGCTAAAGTAGAT+AGG - Chr8:64242351-64242370 None:intergenic 35.0%
GATAATCCTTTCAACGTTGA+AGG + Chr8:64241926-64241945 MsG0880045775.01.T01:CDS 35.0%
GATCTCATAAGTCACACAAT+AGG - Chr8:64242740-64242759 None:intergenic 35.0%
GTAGTCAATCAAATGCCAAT+TGG + Chr8:64243861-64243880 MsG0880045775.01.T01:three_prime_UTR 35.0%
GTGAACTTGGAAAGAAAAAG+TGG - Chr8:64240679-64240698 None:intergenic 35.0%
GTTGTACTGAACTTTATGAC+AGG + Chr8:64243114-64243133 MsG0880045775.01.T01:intron 35.0%
TACAGGCTGCATATAATGTT+GGG + Chr8:64242387-64242406 MsG0880045775.01.T01:intron 35.0%
TATCGAAAAGTGAAGTTGTG+TGG - Chr8:64243390-64243409 None:intergenic 35.0%
TCATTGAAGAAATTCCAACC+TGG - Chr8:64240623-64240642 None:intergenic 35.0%
TTACAGGCTGCATATAATGT+TGG + Chr8:64242386-64242405 MsG0880045775.01.T01:intron 35.0%
TTCAAATGCTTTACGGTACA+AGG - Chr8:64241085-64241104 None:intergenic 35.0%
TTCTGCTAGACTGATTACTT+TGG - Chr8:64241681-64241700 None:intergenic 35.0%
TTGAATCCTTCAACGTTGAA+AGG - Chr8:64241935-64241954 None:intergenic 35.0%
TTGTTTGGACAAATACCTTC+TGG + Chr8:64243020-64243039 MsG0880045775.01.T01:intron 35.0%
TTTCAACGTTGAAGGATTCA+AGG + Chr8:64241934-64241953 MsG0880045775.01.T01:CDS 35.0%
! AAGGCACTTATTGAAGTACT+GGG - Chr8:64241836-64241855 None:intergenic 35.0%
! ACTTTAAGGAATCTTGCTCT+AGG - Chr8:64241183-64241202 None:intergenic 35.0%
! AGACTTTTCACTTTCTTCCA+CGG - Chr8:64241145-64241164 None:intergenic 35.0%
! ATACAGTATGCTGAAAGTGT+TGG + Chr8:64241649-64241668 MsG0880045775.01.T01:intron 35.0%
! GAAAGCTTATTAGCTGTTTC+TGG - Chr8:64241734-64241753 None:intergenic 35.0%
! TATTCTTTATCTGGAGTCTC+AGG - Chr8:64241303-64241322 None:intergenic 35.0%
! TCCATGCATAGATGTAGTTT+TGG + Chr8:64240823-64240842 MsG0880045775.01.T01:intron 35.0%
!! AAAGGCACTTATTGAAGTAC+TGG - Chr8:64241837-64241856 None:intergenic 35.0%
!! TGCACATTAGAAAAAGCACT+TGG + Chr8:64240552-64240571 MsG0880045775.01.T01:CDS 35.0%
!!! ACCTGTTTTAATATGTCGTC+TGG + Chr8:64241514-64241533 MsG0880045775.01.T01:intron 35.0%
!!! CAGCCATTTTTTTTGCAGTA+TGG + Chr8:64240413-64240432 MsG0880045775.01.T01:intron 35.0%
!!! GTGCTTTTTCTAATGTGCAT+CGG - Chr8:64240551-64240570 None:intergenic 35.0%
!!! TCCTTTTTTGGTTAAGACCT+CGG - Chr8:64241214-64241233 None:intergenic 35.0%
AACAGTTGCTCCAGAATTTC+AGG + Chr8:64242032-64242051 MsG0880045775.01.T01:intron 40.0%
AATCCATTCTATGCTCTTCC+CGG - Chr8:64243367-64243386 None:intergenic 40.0%
ACAGGCTGCATATAATGTTG+GGG + Chr8:64242388-64242407 MsG0880045775.01.T01:intron 40.0%
AGACCCACCTATAACTAATC+CGG + Chr8:64241799-64241818 MsG0880045775.01.T01:CDS 40.0%
AGTACATTAGAGCCACGTTT+GGG + Chr8:64243190-64243209 MsG0880045775.01.T01:CDS 40.0%
ATATCTGATACCTAAGGAGC+TGG + Chr8:64243407-64243426 MsG0880045775.01.T01:CDS 40.0%
ATCAGTCTAGCAGAACATCT+CGG + Chr8:64241686-64241705 MsG0880045775.01.T01:CDS 40.0%
ATGTGCATCGGACCTTAAAT+TGG - Chr8:64240539-64240558 None:intergenic 40.0%
CAATTAGAAGACGTAGATCC+AGG + Chr8:64242066-64242085 MsG0880045775.01.T01:intron 40.0%
CTCAGTGTATTGCTCTTTAC+AGG + Chr8:64242370-64242389 MsG0880045775.01.T01:intron 40.0%
CTTCACTTTGCACTTTGTTC+AGG + Chr8:64242876-64242895 MsG0880045775.01.T01:intron 40.0%
GAAGGATTCAAGGAGTTTAG+TGG + Chr8:64241944-64241963 MsG0880045775.01.T01:intron 40.0%
GAGCTATCTTGAAGAGCTTA+TGG + Chr8:64242608-64242627 MsG0880045775.01.T01:intron 40.0%
GCCAGACGACATATTAAAAC+AGG - Chr8:64241518-64241537 None:intergenic 40.0%
TAACTTTCTTCCTCTTGCAG+TGG + Chr8:64242770-64242789 MsG0880045775.01.T01:intron 40.0%
TCAGCTAAAGTAGATAGGCT+AGG - Chr8:64242346-64242365 None:intergenic 40.0%
TCTTCGAAGAAAAGAACACG+CGG - Chr8:64242798-64242817 None:intergenic 40.0%
TGAGACCATCTGCAAATGTT+TGG + Chr8:64243456-64243475 MsG0880045775.01.T01:three_prime_UTR 40.0%
TGATTAATTCTGTGGTTGGC+TGG - Chr8:64241008-64241027 None:intergenic 40.0%
TTGTCTACTGTAACAGATCC+TGG + Chr8:64243722-64243741 MsG0880045775.01.T01:three_prime_UTR 40.0%
! AAGCACTTGTGATGCTGTTT+CGG - Chr8:64242663-64242682 None:intergenic 40.0%
! GAGTCTGAATGCTTTTCTTC+TGG - Chr8:64241339-64241358 None:intergenic 40.0%
! TTACACGCAGTTTTCGATCT+CGG - Chr8:64243693-64243712 None:intergenic 40.0%
!! GAGGAGTTATTTCTTAACCC+TGG - Chr8:64241890-64241909 None:intergenic 40.0%
!! GTCACACACCAGTTGATAAT+TGG - Chr8:64240755-64240774 None:intergenic 40.0%
!! TTGAAGTACTGGGAGATGAA+AGG - Chr8:64241826-64241845 None:intergenic 40.0%
!!! ATTTTAACTAAGGGCCTGTC+TGG - Chr8:64242716-64242735 None:intergenic 40.0%
!!! CCTTTTTTGGTTAAGACCTC+GGG - Chr8:64241213-64241232 None:intergenic 40.0%
AAATGACACGACAATACCCC+AGG + Chr8:64240602-64240621 MsG0880045775.01.T01:CDS 45.0%
ACAAATACCTTCTGGCACTG+TGG + Chr8:64243028-64243047 MsG0880045775.01.T01:intron 45.0%
ACACTCTTTCTCAGGGATTC+AGG - Chr8:64243324-64243343 None:intergenic 45.0%
ACTTACCTCCATCATGGACA+AGG - Chr8:64241471-64241490 None:intergenic 45.0%
AGCATGACTTACCTCCATCA+TGG - Chr8:64241477-64241496 None:intergenic 45.0%
ATTGTATGTTCCACCTGTCC+AGG - Chr8:64242473-64242492 None:intergenic 45.0%
CAGGCTGCATATAATGTTGG+GGG + Chr8:64242389-64242408 MsG0880045775.01.T01:intron 45.0%
CCCGAGGTCTTAACCAAAAA+AGG + Chr8:64241210-64241229 MsG0880045775.01.T01:CDS 45.0%
CTTACCTCCATCATGGACAA+GGG - Chr8:64241470-64241489 None:intergenic 45.0%
GAAGAAGAGACTCAGGATGT+TGG - Chr8:64242860-64242879 None:intergenic 45.0%
GACCCACCTATAACTAATCC+GGG + Chr8:64241800-64241819 MsG0880045775.01.T01:CDS 45.0%
GAGTACATTAGAGCCACGTT+TGG + Chr8:64243189-64243208 MsG0880045775.01.T01:CDS 45.0%
GCAAAGTGAAGAAGAGACTC+AGG - Chr8:64242867-64242886 None:intergenic 45.0%
GCCTTGATCGCCAATAGAAA+AGG - Chr8:64241855-64241874 None:intergenic 45.0%
GGACAAGGGTTGAGAATAAC+AGG - Chr8:64241456-64241475 None:intergenic 45.0%
GGTTTCTGCATTGTATGTGC+CGG + Chr8:64240844-64240863 MsG0880045775.01.T01:intron 45.0%
TAGTTGCACTGCATTCTGTG+AGG - Chr8:64240312-64240331 None:intergenic 45.0%
TCAAAGAAGCTAGTCCAGAC+AGG + Chr8:64242699-64242718 MsG0880045775.01.T01:intron 45.0%
TCACAGAATGCAGTGCAACT+AGG + Chr8:64240311-64240330 MsG0880045775.01.T01:CDS 45.0%
TGGAGGTAAGTCATGCTTCA+TGG + Chr8:64241480-64241499 MsG0880045775.01.T01:intron 45.0%
TTCTCAACCCTTGTCCATGA+TGG + Chr8:64241460-64241479 MsG0880045775.01.T01:intron 45.0%
! AATCCGGGAAGAGCATAGAA+TGG + Chr8:64243361-64243380 MsG0880045775.01.T01:CDS 45.0%
! AGAACTGAGATAAGTACCGC+TGG - Chr8:64242915-64242934 None:intergenic 45.0%
! GATTCAGGTTTGTCTCATGC+TGG + Chr8:64243270-64243289 MsG0880045775.01.T01:CDS 45.0%
! GCCTTTTCTATTGGCGATCA+AGG + Chr8:64241851-64241870 MsG0880045775.01.T01:CDS 45.0%
!! ACCTCAACCATGGTGCTATA+TGG - Chr8:64241971-64241990 None:intergenic 45.0%
!! ACGAGGAGAAGTTAGCTTTC+TGG + Chr8:64242100-64242119 MsG0880045775.01.T01:intron 45.0%
!! CTTGCTCTAGGTGTTGTTAC+AGG - Chr8:64241171-64241190 None:intergenic 45.0%
!! GAAAGGCCCGGATTAGTTAT+AGG - Chr8:64241809-64241828 None:intergenic 45.0%
!! TGGGGTATTGTCGTGTCATT+TGG - Chr8:64240603-64240622 None:intergenic 45.0%
!!! TTTTTGGTTAAGACCTCGGG+TGG - Chr8:64241210-64241229 None:intergenic 45.0%
AATCCACGGTACCTCAACCA+TGG - Chr8:64241981-64242000 None:intergenic 50.0%
ACAACAACTATCTCCCGCTG+TGG + Chr8:64241104-64241123 MsG0880045775.01.T01:CDS 50.0%
AGGAAACCACTCTGATCCAG+CGG + Chr8:64242896-64242915 MsG0880045775.01.T01:intron 50.0%
CCTCGGGTGGAGAAACTTTA+AGG - Chr8:64241197-64241216 None:intergenic 50.0%
CCTTAAAGTTTCTCCACCCG+AGG + Chr8:64241194-64241213 MsG0880045775.01.T01:CDS 50.0%
GCCATATAGCACCATGGTTG+AGG + Chr8:64241967-64241986 MsG0880045775.01.T01:intron 50.0%
GGGATTCAGGTAGAGACTGT+TGG - Chr8:64243311-64243330 None:intergenic 50.0%
TCAACCCTTGTCCATGATGG+AGG + Chr8:64241463-64241482 MsG0880045775.01.T01:intron 50.0%
TCCAGGGAAGTTGAAACACG+AGG + Chr8:64242083-64242102 MsG0880045775.01.T01:intron 50.0%
TCCTCGTGTTTCAACTTCCC+TGG - Chr8:64242087-64242106 None:intergenic 50.0%
TCGACATACGCTGAAGCAAG+AGG + Chr8:64240360-64240379 MsG0880045775.01.T01:CDS 50.0%
TTGATCACCACAGTGCCAGA+AGG - Chr8:64243038-64243057 None:intergenic 50.0%
! TAAGTACCGCTGGATCAGAG+TGG - Chr8:64242905-64242924 None:intergenic 50.0%
! TAGTGGGCCATATAGCACCA+TGG + Chr8:64241961-64241980 MsG0880045775.01.T01:intron 50.0%
! TTGATCTGGTTCCAGGACAC+AGG + Chr8:64240283-64240302 MsG0880045775.01.T01:CDS 50.0%
!! AGGCCCGGATTAGTTATAGG+TGG - Chr8:64241806-64241825 None:intergenic 50.0%
!! GCTCTAGGTGTTGTTACAGG+AGG - Chr8:64241168-64241187 None:intergenic 50.0%
!! GGCCCGGATTAGTTATAGGT+GGG - Chr8:64241805-64241824 None:intergenic 50.0%
AATGTCTCGTCCTGGACAGG+TGG + Chr8:64242460-64242479 MsG0880045775.01.T01:intron 55.0%
AGATGCAGATGCAGCCACAG+CGG - Chr8:64241121-64241140 None:intergenic 55.0%
CCTGTCCAGGACGAGACATT+CGG - Chr8:64242460-64242479 None:intergenic 55.0%
CTGTGAGGTAACCTGTGTCC+TGG - Chr8:64240297-64240316 None:intergenic 55.0%
GACACGACAATACCCCAGGT+TGG + Chr8:64240606-64240625 MsG0880045775.01.T01:intron 55.0%
GTACTGGGAGATGAAAGGCC+CGG - Chr8:64241821-64241840 None:intergenic 55.0%
GTCCTTGCGTATCCCAAACG+TGG - Chr8:64243205-64243224 None:intergenic 55.0%
! AGCCACGTTTGGGATACGCA+AGG + Chr8:64243200-64243219 MsG0880045775.01.T01:CDS 55.0%
AAGGCGACACGTGGAGTCCA+GGG + Chr8:64241870-64241889 MsG0880045775.01.T01:CDS 60.0%
ACACGCGGAACCACTGCAAG+AGG - Chr8:64242783-64242802 None:intergenic 60.0%
CCGAATGTCTCGTCCTGGAC+AGG + Chr8:64242457-64242476 MsG0880045775.01.T01:intron 60.0%
GATGCAGATGCAGCCACAGC+GGG - Chr8:64241120-64241139 None:intergenic 60.0%
GGCTGCATCTGCATCTACCG+TGG + Chr8:64241125-64241144 MsG0880045775.01.T01:CDS 60.0%
TTGGCGATCAAGGCGACACG+TGG + Chr8:64241861-64241880 MsG0880045775.01.T01:CDS 60.0%
!! GCACCATGGTTGAGGTACCG+TGG + Chr8:64241975-64241994 MsG0880045775.01.T01:intron 60.0%
CAAGGCGACACGTGGAGTCC+AGG + Chr8:64241869-64241888 MsG0880045775.01.T01:CDS 65.0%
!! GGGTGCCGAATGTCTCGTCC+TGG + Chr8:64242452-64242471 MsG0880045775.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 64240277 64243965 64240277 ID=MsG0880045775.01;Name=MsG0880045775.01
Chr8 mRNA 64240277 64243965 64240277 ID=MsG0880045775.01.T01;Parent=MsG0880045775.01;Name=MsG0880045775.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0.6|0.5|1|0.8|0.66|6|479|411
Chr8 exon 64240277 64240381 64240277 ID=MsG0880045775.01.T01:exon:13358;Parent=MsG0880045775.01.T01
Chr8 exon 64240507 64240623 64240507 ID=MsG0880045775.01.T01:exon:13359;Parent=MsG0880045775.01.T01
Chr8 exon 64241009 64241412 64241009 ID=MsG0880045775.01.T01:exon:13360;Parent=MsG0880045775.01.T01
Chr8 exon 64241655 64241962 64241655 ID=MsG0880045775.01.T01:exon:13361;Parent=MsG0880045775.01.T01
Chr8 exon 64243136 64243535 64243136 ID=MsG0880045775.01.T01:exon:13362;Parent=MsG0880045775.01.T01
Chr8 exon 64243585 64243965 64243585 ID=MsG0880045775.01.T01:exon:13363;Parent=MsG0880045775.01.T01
Chr8 CDS 64240277 64240381 64240277 ID=MsG0880045775.01.T01:cds;Parent=MsG0880045775.01.T01
Chr8 CDS 64240507 64240623 64240507 ID=MsG0880045775.01.T01:cds;Parent=MsG0880045775.01.T01
Chr8 CDS 64241009 64241412 64241009 ID=MsG0880045775.01.T01:cds;Parent=MsG0880045775.01.T01
Chr8 CDS 64241655 64241962 64241655 ID=MsG0880045775.01.T01:cds;Parent=MsG0880045775.01.T01
Chr8 CDS 64243136 64243437 64243136 ID=MsG0880045775.01.T01:cds;Parent=MsG0880045775.01.T01
Chr8 three_prime_UTR 64243438 64243535 64243438 ID=MsG0880045775.01.T01:three_prime_utr;Parent=MsG0880045775.01.T01
Chr8 three_prime_UTR 64243585 64243965 64243585 ID=MsG0880045775.01.T01:three_prime_utr;Parent=MsG0880045775.01.T01
Gene Sequence

>MsG0880045775.01.T01

ATGAATTTGATCTGGTTCCAGGACACAGGTTACCTCACAGAATGCAGTGCAACTAGGAAAAAACAAACCCCTATGAATAATGTTCGACATACGCTGAAGCAAGAGATTCTACAATTAGAGAAAAGACTAGAAGACCAATTTAAGGTCCGATGCACATTAGAAAAAGCACTTGGATACAGACCCATATCTCTTGTTAATCCAAATGACACGACAATACCCCAGCCAACCACAGAATTAATCAAAGACATTGCAGTTTTAGAGTTAGAAGTTGTATATTTGGAACAATATCTTCTCTCCTTGTACCGTAAAGCATTTGAACAACAACTATCTCCCGCTGTGGCTGCATCTGCATCTACCGTGGAAGAAAGTGAAAAGTCTCCTCCTGTAACAACACCTAGAGCAAGATTCCTTAAAGTTTCTCCACCCGAGGTCTTAACCAAAAAAGGATGTTCAGATGTACAATGTATTGATCATGAGATTCATACACTTCAAAAGGAATGTAATGGATATAAACCTGAGACTCCAGATAAAGAATACATTGTACATCATCCAGAAGAAAAGCATTCAGACTCTAGCGTACATCGATGTCACTCCTCGTTATCCCAATATCAACATCTACAGCAAAATATGCTGAAAGTGTTGGCTTCAACCAAAGTAATCAGTCTAGCAGAACATCTCGGTACCCGAATATGTGATCATATTCCAGAAACAGCTAATAAGCTTTCTGAGGATATGGTTAAATGTATATCAGCTATATATTACAAACTTGCAGACCCACCTATAACTAATCCGGGCCTTTCATCTCCCAGTACTTCAATAAGTGCCTTTTCTATTGGCGATCAAGGCGACACGTGGAGTCCAGGGTTAAGAAATAACTCCTCTTTTGATGTACAGTTAGATAATCCTTTCAACGTTGAAGGATTCAAGGAGTTTAGTACGTGTATATACACCGAAGAGAGTGTTTCAAGAACTAGAATTGCAAAGGAAGAGTACATTAGAGCCACGTTTGGGATACGCAAGGACCAGAAAATACTTCTACCTAAGATTGTAGATACATTTGTCTCCAAAGATTCAGGTTTGTCTCATGCTGGTCTTATAGAGATGATCCAACAGTCTCTACCTGAATCCCTGAGAAAGAGTGTTAAGAAATGTCATGCAAAATCCGGGAAGAGCATAGAATGGATTCCACACAACTTCACTTTTCGATATCTGATACCTAAGGAGCTGGTTAAATAA
Protein sequence

>MsG0880045775.01.T01

MNLIWFQDTGYLTECSATRKKQTPMNNVRHTLKQEILQLEKRLEDQFKVRCTLEKALGYRPISLVNPNDTTIPQPTTELIKDIAVLELEVVYLEQYLLSLYRKAFEQQLSPAVAASASTVEESEKSPPVTTPRARFLKVSPPEVLTKKGCSDVQCIDHEIHTLQKECNGYKPETPDKEYIVHHPEEKHSDSSVHRCHSSLSQYQHLQQNMLKVLASTKVISLAEHLGTRICDHIPETANKLSEDMVKCISAIYYKLADPPITNPGLSSPSTSISAFSIGDQGDTWSPGLRNNSSFDVQLDNPFNVEGFKEFSTCIYTEESVSRTRIAKEEYIRATFGIRKDQKILLPKIVDTFVSKDSGLSHAGLIEMIQQSLPESLRKSVKKCHAKSGKSIEWIPHNFTFRYLIPKELVK*