AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024405.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024405.01.T01 MTR_5g010590 80.095 211 36 3 1 207 1 209 4.44e-100 288
MsG0580024405.01.T01 MTR_5g010590 66.316 190 18 1 1 144 1 190 8.39e-65 199
MsG0580024405.01.T01 MTR_8g097320 37.383 214 101 6 10 207 4 200 5.56e-31 112
MsG0580024405.01.T01 MTR_8g097320 36.866 217 101 7 10 207 4 203 8.48e-29 107
MsG0580024405.01.T01 MTR_1g100647 48.031 127 62 2 82 205 84 209 1.41e-27 103
MsG0580024405.01.T01 MTR_8g031370 46.875 128 62 3 82 205 75 200 4.40e-24 94.7
MsG0580024405.01.T01 MTR_7g076730 40.310 129 71 3 81 205 7 133 1.37e-19 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024405.01.T01 AT5G23750 48.819 127 61 2 82 205 104 229 6.18e-31 113
MsG0580024405.01.T01 AT5G23750 48.819 127 61 2 82 205 76 201 7.25e-31 112
MsG0580024405.01.T01 AT5G23750 48.819 127 61 2 82 205 77 202 8.02e-31 112
MsG0580024405.01.T01 AT3G61260 42.748 131 71 2 78 205 83 212 1.18e-23 94.0
MsG0580024405.01.T01 AT3G48940 43.307 127 68 2 82 205 50 175 2.32e-23 92.4
MsG0580024405.01.T01 AT2G45820 40.940 149 58 3 73 205 56 190 3.38e-22 89.7

Find 39 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCAGTTCTTCTTCTTATT+TGG 0.151302 5:-4406329 None:intergenic
AAGCATGCAAACAATTTCTT+TGG 0.279239 5:-4406285 None:intergenic
AGGGGATCATGATGATAAAA+AGG 0.323089 5:+4405416 MsG0580024405.01.T01:CDS
GTTGAAATGGCCAGCTGATT+AGG 0.351901 5:-4404652 None:intergenic
AGATGATCATGCTGGTAATA+AGG 0.356783 5:+4405389 MsG0580024405.01.T01:CDS
CATGCTGGTAATAAGGAAAC+AGG 0.363454 5:+4405396 MsG0580024405.01.T01:CDS
GGGGAAAGCTCTTCAGGAAC+AGG 0.393746 5:-4404577 None:intergenic
GCTTTCCCCTGTTGTGGAAA+AGG 0.394410 5:+4404591 MsG0580024405.01.T01:CDS
CTGATCATCAAGTGTTGAAA+TGG 0.399507 5:-4404665 None:intergenic
ACAACAGGGGAAAGCTCTTC+AGG 0.423226 5:-4404583 None:intergenic
TTCTGTTCTGTTATGTTCTG+TGG 0.423547 5:-4404482 None:intergenic
ACCACAGCTACAGTCTCTTC+TGG 0.424564 5:+4404552 MsG0580024405.01.T01:CDS
TGGCCAGCTGATTAGGCTCC+TGG 0.459792 5:-4404645 None:intergenic
TGAAGAGCTTTCCCCTGTTG+TGG 0.460075 5:+4404585 MsG0580024405.01.T01:CDS
AATTGTAGCAGAGAAAAGAT+TGG 0.480532 5:+4405564 MsG0580024405.01.T01:CDS
ATTGGCTTTAATTAAAGCAT+GGG 0.513985 5:+4405582 MsG0580024405.01.T01:CDS
GATTGGCTTTAATTAAAGCA+TGG 0.523135 5:+4405581 MsG0580024405.01.T01:CDS
ATGTTATTGATTATGAAATG+TGG 0.536366 5:-4406433 None:intergenic
CAGCCAGGAGCCTAATCAGC+TGG 0.541152 5:+4404642 MsG0580024405.01.T01:CDS
AGGCTCCTGGCTGATACTGA+TGG 0.554814 5:-4404632 None:intergenic
TAACAGAACAGAACAAGTCA+TGG 0.567812 5:+4404492 MsG0580024405.01.T01:exon
CTGAAAACTTAGTTATGACA+AGG 0.584476 5:-4404441 None:intergenic
AACTTAGTTATGACAAGGGT+AGG 0.585109 5:-4404436 None:intergenic
GGAAGAATGTCTGGAGCTAG+AGG 0.585162 5:+4406227 MsG0580024405.01.T01:CDS
CGACACATTTCAGTTCCACA+AGG 0.586177 5:-4405898 None:intergenic
GACAAATCTCTCGAGCCTTG+TGG 0.588467 5:+4405883 MsG0580024405.01.T01:CDS
AAGCACCATCAGTATCAGCC+AGG 0.590861 5:+4404627 MsG0580024405.01.T01:CDS
ATTTCTTTGGTGCAACTCCA+CGG 0.591194 5:-4406272 None:intergenic
GCTGCAAAGTTTCGTAGCCG+TGG 0.595886 5:+4406255 MsG0580024405.01.T01:CDS
GAGGTTGCAGATGATCATGC+TGG 0.615505 5:+4405381 MsG0580024405.01.T01:intron
AACAGAACAGAACAAGTCAT+GGG 0.617130 5:+4404493 MsG0580024405.01.T01:exon
CAAGTCATGGGAGAATCAGA+AGG 0.618923 5:+4404505 MsG0580024405.01.T01:exon
TCCAGAAGAGACTGTAGCTG+TGG 0.625821 5:-4404553 None:intergenic
ATGCTGGTAATAAGGAAACA+GGG 0.632785 5:+4405397 MsG0580024405.01.T01:CDS
TGCTGGTAATAAGGAAACAG+GGG 0.640080 5:+4405398 MsG0580024405.01.T01:CDS
GGGAAAGCTCTTCAGGAACA+GGG 0.646598 5:-4404576 None:intergenic
TTAGTTATGACAAGGGTAGG+TGG 0.647579 5:-4404433 None:intergenic
GCTAAGGGCTCAACTGACAG+AGG 0.669431 5:+4405441 MsG0580024405.01.T01:CDS
TGAAAACTTAGTTATGACAA+GGG 0.676390 5:-4404440 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTATTAGTTATTATTTA+TGG + Chr5:4405799-4405818 MsG0580024405.01.T01:intron 10.0%
!!! ACAAAATTGTTTTAAATAAT+AGG + Chr5:4406095-4406114 MsG0580024405.01.T01:intron 10.0%
!!! TTTTAAATAATAGGAAAATT+TGG + Chr5:4406104-4406123 MsG0580024405.01.T01:intron 10.0%
!! AATGAATATTATGATGATTT+AGG - Chr5:4404411-4404430 None:intergenic 15.0%
!! TATCGTATTTATAGTTAATA+TGG + Chr5:4405201-4405220 MsG0580024405.01.T01:intron 15.0%
!!! ATATTTTATATGAGTAAGAA+TGG - Chr5:4405647-4405666 None:intergenic 15.0%
!!! TATTTTATATGAGTAAGAAT+GGG - Chr5:4405646-4405665 None:intergenic 15.0%
!! GTAATATTGATGAATAAAGA+AGG - Chr5:4406031-4406050 None:intergenic 20.0%
!! TTTAAACCTCCAAATATATT+AGG - Chr5:4405040-4405059 None:intergenic 20.0%
!!! AATTTTGTAAGTTACAAATG+AGG - Chr5:4406083-4406102 None:intergenic 20.0%
!!! ATGTTATTGATTATGAAATG+TGG - Chr5:4406436-4406455 None:intergenic 20.0%
!!! ATTTTGTAAGTTACAAATGA+GGG - Chr5:4406082-4406101 None:intergenic 20.0%
!!! GTTAATATGGAAATTTTTTG+AGG + Chr5:4405214-4405233 MsG0580024405.01.T01:intron 20.0%
!!! GTTTTAGACTTCAAAATTTT+AGG - Chr5:4405251-4405270 None:intergenic 20.0%
!!! GTTTTGTTTTGTTGTAAATT+AGG + Chr5:4405838-4405857 MsG0580024405.01.T01:intron 20.0%
!!! TTATGTATACTTTTAAAGTG+TGG + Chr5:4406371-4406390 MsG0580024405.01.T01:three_prime_UTR 20.0%
! AATAGTTGAAGCTCAAAAAA+GGG + Chr5:4406206-4406225 MsG0580024405.01.T01:CDS 25.0%
! AGCTTAATTGTTTAGAAAGA+AGG - Chr5:4405154-4405173 None:intergenic 25.0%
! ATTCAGTTCTTCTTCTTATT+TGG - Chr5:4406332-4406351 None:intergenic 25.0%
! CCTAGAACAAATAAAATATG+AGG + Chr5:4405128-4405147 MsG0580024405.01.T01:intron 25.0%
! TGAAAACTTAGTTATGACAA+GGG - Chr5:4404443-4404462 None:intergenic 25.0%
!! CTAACTTGTCTAAATTCTTA+AGG + Chr5:4404998-4405017 MsG0580024405.01.T01:intron 25.0%
!! TCATCATATTTTTCATACAG+AGG + Chr5:4405362-4405381 MsG0580024405.01.T01:intron 25.0%
!!! ATGAATAAAGAAGGTATTGT+GGG - Chr5:4406022-4406041 None:intergenic 25.0%
!!! ATTGGCTTTAATTAAAGCAT+GGG + Chr5:4405582-4405601 MsG0580024405.01.T01:CDS 25.0%
!!! CCTCATATTTTATTTGTTCT+AGG - Chr5:4405131-4405150 None:intergenic 25.0%
!!! TCTCTTTATTTACTGTCTTT+TGG + Chr5:4404705-4404724 MsG0580024405.01.T01:intron 25.0%
AAAAGACAAAGGCAGAAAAT+AGG + Chr5:4405614-4405633 MsG0580024405.01.T01:intron 30.0%
AAATTCTTAAGGTCATTCTC+AGG + Chr5:4405009-4405028 MsG0580024405.01.T01:intron 30.0%
AAGCAAGAAATCATCTATAG+AGG + Chr5:4405755-4405774 MsG0580024405.01.T01:intron 30.0%
AAGCATGCAAACAATTTCTT+TGG - Chr5:4406288-4406307 None:intergenic 30.0%
AATTCTTAAGGTCATTCTCA+GGG + Chr5:4405010-4405029 MsG0580024405.01.T01:intron 30.0%
AATTGTAGCAGAGAAAAGAT+TGG + Chr5:4405564-4405583 MsG0580024405.01.T01:CDS 30.0%
CAATAGTTGAAGCTCAAAAA+AGG + Chr5:4406205-4406224 MsG0580024405.01.T01:CDS 30.0%
CTGAAAACTTAGTTATGACA+AGG - Chr5:4404444-4404463 None:intergenic 30.0%
GATGATAAAAAGGATGCTAA+GGG + Chr5:4405426-4405445 MsG0580024405.01.T01:CDS 30.0%
TCTAGTATAACGAAGGTTTA+GGG - Chr5:4405977-4405996 None:intergenic 30.0%
TGATGATAAAAAGGATGCTA+AGG + Chr5:4405425-4405444 MsG0580024405.01.T01:CDS 30.0%
TTCTAGTATAACGAAGGTTT+AGG - Chr5:4405978-4405997 None:intergenic 30.0%
! ATTTTGTTGCATTAGATGCT+GGG + Chr5:4405533-4405552 MsG0580024405.01.T01:intron 30.0%
! CTTGTCATAACTAAGTTTTC+AGG + Chr5:4404442-4404461 MsG0580024405.01.T01:five_prime_UTR 30.0%
! GAAGTCTAAAACCTTAACTT+GGG + Chr5:4405259-4405278 MsG0580024405.01.T01:intron 30.0%
! TGAAGTCTAAAACCTTAACT+TGG + Chr5:4405258-4405277 MsG0580024405.01.T01:intron 30.0%
!! GATGAATAAAGAAGGTATTG+TGG - Chr5:4406023-4406042 None:intergenic 30.0%
!! GATTGGCTTTAATTAAAGCA+TGG + Chr5:4405581-4405600 MsG0580024405.01.T01:CDS 30.0%
!! TGATTCATTTTTGTTGATGC+TGG - Chr5:4404533-4404552 None:intergenic 30.0%
AACAGAACAGAACAAGTCAT+GGG + Chr5:4404493-4404512 MsG0580024405.01.T01:exon 35.0%
AACTTAGTTATGACAAGGGT+AGG - Chr5:4404439-4404458 None:intergenic 35.0%
AGATGATCATGCTGGTAATA+AGG + Chr5:4405389-4405408 MsG0580024405.01.T01:CDS 35.0%
AGCAATCTTCTGTTGGATTA+TGG + Chr5:4405727-4405746 MsG0580024405.01.T01:intron 35.0%
AGGGGATCATGATGATAAAA+AGG + Chr5:4405416-4405435 MsG0580024405.01.T01:CDS 35.0%
ATGCTGGTAATAAGGAAACA+GGG + Chr5:4405397-4405416 MsG0580024405.01.T01:CDS 35.0%
GAGCTTGATGAAAGATGAAA+TGG + Chr5:4405685-4405704 MsG0580024405.01.T01:intron 35.0%
GATCTCTTCTAGTATAACGA+AGG - Chr5:4405984-4406003 None:intergenic 35.0%
GCAATCTTCTGTTGGATTAT+GGG + Chr5:4405728-4405747 MsG0580024405.01.T01:intron 35.0%
GGAAGAAAGTGAAAAGACAA+AGG + Chr5:4405603-4405622 MsG0580024405.01.T01:intron 35.0%
TAACAGAACAGAACAAGTCA+TGG + Chr5:4404492-4404511 MsG0580024405.01.T01:exon 35.0%
TACAAGAAGCAATCTTCTGT+TGG + Chr5:4405720-4405739 MsG0580024405.01.T01:intron 35.0%
TCAAAAAAGGGAAGAATGTC+TGG + Chr5:4406218-4406237 MsG0580024405.01.T01:CDS 35.0%
TTCTGTTCTGTTATGTTCTG+TGG - Chr5:4404485-4404504 None:intergenic 35.0%
! CATTTTGTTGCATTAGATGC+TGG + Chr5:4405532-4405551 MsG0580024405.01.T01:intron 35.0%
!! CTGATCATCAAGTGTTGAAA+TGG - Chr5:4404668-4404687 None:intergenic 35.0%
AAAAGCTCATGCAGTGATCA+GGG - Chr5:4405305-4405324 None:intergenic 40.0%
AAAGATGAAATGGTCACGCA+GGG + Chr5:4405695-4405714 MsG0580024405.01.T01:intron 40.0%
ACAAAGGCAGAAAATAGGCA+AGG + Chr5:4405619-4405638 MsG0580024405.01.T01:intron 40.0%
AGAAGAGATCTAAGCACTCT+TGG + Chr5:4405994-4406013 MsG0580024405.01.T01:intron 40.0%
ATCTTCTGTTGGATTATGGG+AGG + Chr5:4405731-4405750 MsG0580024405.01.T01:intron 40.0%
CAGGGACGACCTAATATATT+TGG + Chr5:4405028-4405047 MsG0580024405.01.T01:intron 40.0%
CATGCTGGTAATAAGGAAAC+AGG + Chr5:4405396-4405415 MsG0580024405.01.T01:CDS 40.0%
GAAGAGATCTAAGCACTCTT+GGG + Chr5:4405995-4406014 MsG0580024405.01.T01:intron 40.0%
GGACGACCTAATATATTTGG+AGG + Chr5:4405031-4405050 MsG0580024405.01.T01:intron 40.0%
GGCACAACTCAAGAAATTTG+AGG + Chr5:4405776-4405795 MsG0580024405.01.T01:intron 40.0%
TGCTGGTAATAAGGAAACAG+GGG + Chr5:4405398-4405417 MsG0580024405.01.T01:CDS 40.0%
TTAGTTATGACAAGGGTAGG+TGG - Chr5:4404436-4404455 None:intergenic 40.0%
! AAACCTTAACTTGGGTTGCT+TGG + Chr5:4405267-4405286 MsG0580024405.01.T01:intron 40.0%
! ATTTCTTTGGTGCAACTCCA+CGG - Chr5:4406275-4406294 None:intergenic 40.0%
! CTGATTCCTTTTCCACAACA+GGG - Chr5:4404600-4404619 None:intergenic 40.0%
! TCTGATTCCTTTTCCACAAC+AGG - Chr5:4404601-4404620 None:intergenic 40.0%
! TGATTCCTTTTCCACAACAG+GGG - Chr5:4404599-4404618 None:intergenic 40.0%
CAAAAGCTCATGCAGTGATC+AGG - Chr5:4405306-4405325 None:intergenic 45.0%
CAAGTCATGGGAGAATCAGA+AGG + Chr5:4404505-4404524 MsG0580024405.01.T01:exon 45.0%
CGACACATTTCAGTTCCACA+AGG - Chr5:4405901-4405920 None:intergenic 45.0%
GAAAGATGAAATGGTCACGC+AGG + Chr5:4405694-4405713 MsG0580024405.01.T01:intron 45.0%
GTTGAAATGGCCAGCTGATT+AGG - Chr5:4404655-4404674 None:intergenic 45.0%
AAGCACCATCAGTATCAGCC+AGG + Chr5:4404627-4404646 MsG0580024405.01.T01:CDS 50.0%
ACAACAGGGGAAAGCTCTTC+AGG - Chr5:4404586-4404605 None:intergenic 50.0%
ACCACAGCTACAGTCTCTTC+TGG + Chr5:4404552-4404571 MsG0580024405.01.T01:CDS 50.0%
GACAAATCTCTCGAGCCTTG+TGG + Chr5:4405883-4405902 MsG0580024405.01.T01:CDS 50.0%
GAGGTTGCAGATGATCATGC+TGG + Chr5:4405381-4405400 MsG0580024405.01.T01:intron 50.0%
GCTTTCCCCTGTTGTGGAAA+AGG + Chr5:4404591-4404610 MsG0580024405.01.T01:CDS 50.0%
GGGAAAGCTCTTCAGGAACA+GGG - Chr5:4404579-4404598 None:intergenic 50.0%
TCCAGAAGAGACTGTAGCTG+TGG - Chr5:4404556-4404575 None:intergenic 50.0%
TGAAGAGCTTTCCCCTGTTG+TGG + Chr5:4404585-4404604 MsG0580024405.01.T01:CDS 50.0%
! GGAAGAATGTCTGGAGCTAG+AGG + Chr5:4406227-4406246 MsG0580024405.01.T01:CDS 50.0%
AGGCTCCTGGCTGATACTGA+TGG - Chr5:4404635-4404654 None:intergenic 55.0%
GCTAAGGGCTCAACTGACAG+AGG + Chr5:4405441-4405460 MsG0580024405.01.T01:CDS 55.0%
GCTCATGCAGTGATCAGGGT+TGG - Chr5:4405301-4405320 None:intergenic 55.0%
GCTGCAAAGTTTCGTAGCCG+TGG + Chr5:4406255-4406274 MsG0580024405.01.T01:CDS 55.0%
GGGCCAAGCAACCCAAGTTA+AGG - Chr5:4405273-4405292 None:intergenic 55.0%
GGGGAAAGCTCTTCAGGAAC+AGG - Chr5:4404580-4404599 None:intergenic 55.0%
! AACTTGGGTTGCTTGGCCCT+CGG + Chr5:4405274-4405293 MsG0580024405.01.T01:intron 55.0%
CAGCCAGGAGCCTAATCAGC+TGG + Chr5:4404642-4404661 MsG0580024405.01.T01:CDS 60.0%
TGGCCAGCTGATTAGGCTCC+TGG - Chr5:4404648-4404667 None:intergenic 60.0%
! ACTTGGGTTGCTTGGCCCTC+GGG + Chr5:4405275-4405294 MsG0580024405.01.T01:intron 60.0%
!! AGTGATCAGGGTTGGCCCGA+GGG - Chr5:4405293-4405312 None:intergenic 60.0%
!! CAGTGATCAGGGTTGGCCCG+AGG - Chr5:4405294-4405313 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 4404411 4406469 4404411 ID=MsG0580024405.01;Name=MsG0580024405.01
Chr5 mRNA 4404411 4406469 4404411 ID=MsG0580024405.01.T01;Parent=MsG0580024405.01;Name=MsG0580024405.01.T01;_AED=0.43;_eAED=0.49;_QI=100|0.5|0.6|1|1|1|5|152|207
Chr5 exon 4404411 4404688 4404411 ID=MsG0580024405.01.T01:exon:28982;Parent=MsG0580024405.01.T01
Chr5 exon 4405382 4405462 4405382 ID=MsG0580024405.01.T01:exon:28983;Parent=MsG0580024405.01.T01
Chr5 exon 4405548 4405610 4405548 ID=MsG0580024405.01.T01:exon:28984;Parent=MsG0580024405.01.T01
Chr5 exon 4405860 4405960 4405860 ID=MsG0580024405.01.T01:exon:28985;Parent=MsG0580024405.01.T01
Chr5 exon 4406117 4406469 4406117 ID=MsG0580024405.01.T01:exon:28986;Parent=MsG0580024405.01.T01
Chr5 five_prime_UTR 4404411 4404510 4404411 ID=MsG0580024405.01.T01:five_prime_utr;Parent=MsG0580024405.01.T01
Chr5 CDS 4404511 4404688 4404511 ID=MsG0580024405.01.T01:cds;Parent=MsG0580024405.01.T01
Chr5 CDS 4405382 4405462 4405382 ID=MsG0580024405.01.T01:cds;Parent=MsG0580024405.01.T01
Chr5 CDS 4405548 4405610 4405548 ID=MsG0580024405.01.T01:cds;Parent=MsG0580024405.01.T01
Chr5 CDS 4405860 4405960 4405860 ID=MsG0580024405.01.T01:cds;Parent=MsG0580024405.01.T01
Chr5 CDS 4406117 4406317 4406117 ID=MsG0580024405.01.T01:cds;Parent=MsG0580024405.01.T01
Chr5 three_prime_UTR 4406318 4406469 4406318 ID=MsG0580024405.01.T01:three_prime_utr;Parent=MsG0580024405.01.T01
Gene Sequence

>MsG0580024405.01.T01

ATGGGAGAATCAGAAGGTTCCAGCATCAACAAAAATGAATCACCACAGCTACAGTCTCTTCTGGACCCTGTTCCTGAAGAGCTTTCCCCTGTTGTGGAAAAGGAATCAGATACTCAAAGCACCATCAGTATCAGCCAGGAGCCTAATCAGCTGGCCATTTCAACACTTGATGATCAGAAGGTTGCAGATGATCATGCTGGTAATAAGGAAACAGGGGATCATGATGATAAAAAGGATGCTAAGGGCTCAACTGACAGAGATGCTGGGCTTGCAAAAATTGTAGCAGAGAAAAGATTGGCTTTAATTAAAGCATGGGAAGAAAGTATCTTTGCATGCAAGTGTATAGACAAATCTCTCGAGCCTTGTGGAACTGAAATGTGTCGCAAATTCTTTCTAAAAGTTTTATCTCTGTTACATGCACAAGAAAATTTGGAAAGAAAGAAAGTTGAGTATATTTCAAAAATGAAAAATGAACTAGCTGAAATTCATCAATATGCTGAAGAGAAAAGAGCAATAGTTGAAGCTCAAAAAAGGGAAGAATGTCTGGAGCTAGAGGAAACAGCTGCAAAGTTTCGTAGCCGTGGAGTTGCACCAAAGAAATTGTTTGCATGCTTTAGCGCTTAA

Protein sequence

>MsG0580024405.01.T01

MGESEGSSINKNESPQLQSLLDPVPEELSPVVEKESDTQSTISISQEPNQLAISTLDDQKVADDHAGNKETGDHDDKKDAKGSTDRDAGLAKIVAEKRLALIKAWEESIFACKCIDKSLEPCGTEMCRKFFLKVLSLLHAQENLERKKVEYISKMKNELAEIHQYAEEKRAIVEAQKREECLELEETAAKFRSRGVAPKKLFACFSA*