AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0036670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0036670 MtrunA17_Chr1g0199211 95.238 294 14 0 1 294 5 298 0.0 579
Msa0036670 MtrunA17_Chr1g0171391 60.643 249 92 3 1 248 1 244 1.74e-102 299
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0036670 AT2G39170.1 41.833 251 106 4 1 248 1 214 5.24e-50 165

Find 86 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAATAAAGTCTGATATTT+TGG 0.099654 1_1:+65259385 None:intergenic
CTTCTCTTCGGTTGTTATTA+TGG 0.182520 1_1:+65262925 None:intergenic
CAGGCTTATCTTGGTGGTTC+AGG 0.214403 1_1:+65259707 None:intergenic
GTTCTTTCTGTCTGAGAATT+TGG 0.214548 1_1:+65260416 None:intergenic
CATGAATTCTCTACCGTTGA+TGG 0.268118 1_1:-65260758 Msa0036670:CDS
GTGGCCACATGTGACATTTA+TGG 0.295550 1_1:-65259442 Msa0036670:three_prime_UTR
CAACAACAATATCATATAAC+AGG 0.309074 1_1:+65259587 None:intergenic
TGGCAGAACTTCATCATTAC+AGG 0.309722 1_1:-65260198 Msa0036670:CDS
ATATTGTTGTTGAAATGTTC+AGG 0.315653 1_1:-65259576 Msa0036670:three_prime_UTR
CACTTTGCAAATCATATTAT+CGG 0.318847 1_1:+65259500 None:intergenic
AACCTCTAGACACATCAATT+AGG 0.346493 1_1:+65262330 None:intergenic
ACCTATCATCTCATTGACTA+TGG 0.347470 1_1:+65260514 None:intergenic
AATGCTGATATCACTTTCAT+TGG 0.349970 1_1:+65260112 None:intergenic
TTGTCAGCTTTAATGACATT+TGG 0.384892 1_1:+65260632 None:intergenic
ACCTCTAGACACATCAATTA+GGG 0.395040 1_1:+65262331 None:intergenic
TCTTCAACCTGACTCGATAT+AGG 0.401910 1_1:+65260155 None:intergenic
AACGAGGAGAAGATAAATAC+TGG 0.403101 1_1:-65259774 Msa0036670:CDS
AGGCTTATCTTGGTGGTTCA+GGG 0.406428 1_1:+65259708 None:intergenic
GTGATTGACCGTGGCAAATC+AGG 0.414138 1_1:+65260227 None:intergenic
TGTTACAACCAGGCTTATCT+TGG 0.414647 1_1:+65259698 None:intergenic
CTTCGGTTGTTATTATGGTA+TGG 0.416328 1_1:+65262930 None:intergenic
TCCATAGTCAATGAGATGAT+AGG 0.429467 1_1:-65260515 Msa0036670:CDS
GTCACTGTCGTGTCGTGTGT+GGG 0.441088 1_1:-65262979 Msa0036670:five_prime_UTR
CTAAACGAGTTATTGCTGTT+GGG 0.442255 1_1:-65263014 None:intergenic
AACTTTGCACACTGAAGACT+TGG 0.451922 1_1:-65260582 Msa0036670:CDS
TTGGTGTTGATGTTGATGAA+TGG 0.456620 1_1:+65260435 None:intergenic
AGGTGACATAAACAAATCTC+TGG 0.458453 1_1:-65260495 Msa0036670:CDS
AAACTAGAGGAGTGGCTTGA+AGG 0.458965 1_1:-65260035 Msa0036670:CDS
AGTTGTTGTCATTGTCAGAA+AGG 0.459938 1_1:-65260088 Msa0036670:CDS
GCTAAACGAGTTATTGCTGT+TGG 0.460300 1_1:-65263015 None:intergenic
AGACTCATGACCCTTCTCTT+CGG 0.461811 1_1:+65262913 None:intergenic
TGATTTGCCTATATCGAGTC+AGG 0.466514 1_1:-65260162 Msa0036670:CDS
TACAACCAGGCTTATCTTGG+TGG 0.469510 1_1:+65259701 None:intergenic
ATATCGTAGCTTCTCTTCGT+TGG 0.471398 1_1:+65260250 None:intergenic
ACTTTGCAAATCATATTATC+GGG 0.477352 1_1:+65259501 None:intergenic
CATAATAACAACCGAAGAGA+AGG 0.495077 1_1:-65262924 Msa0036670:five_prime_UTR
GTGGCTAACGAACCATCTGC+GGG 0.500766 1_1:-65260692 Msa0036670:CDS
GTGGCCACCAAAACATGAAA+AGG 0.502675 1_1:+65259457 None:intergenic
CTCGATATAGGCAAATCATC+AGG 0.503555 1_1:+65260167 None:intergenic
TTTGCACACTGAAGACTTGG+AGG 0.512330 1_1:-65260579 Msa0036670:CDS
TCTACTGATCCTTTACCATC+AGG 0.516231 1_1:+65260275 None:intergenic
GTTGATGTTGATGAATGGAT+TGG 0.527813 1_1:+65260440 None:intergenic
TGGCAACAGCCTGATGGTAA+AGG 0.529712 1_1:-65260284 Msa0036670:CDS
TATAGGCAAATCATCAGGTT+CGG 0.532495 1_1:+65260172 None:intergenic
ACATTCTTTCATTGATCGAA+CGG 0.535936 1_1:+65260544 None:intergenic
TGTCACTGTCGTGTCGTGTG+TGG 0.539186 1_1:-65262980 Msa0036670:five_prime_UTR
TAAACGAGTTATTGCTGTTG+GGG 0.546233 1_1:-65263013 None:intergenic
AACATGTTACTCAATTTCAA+AGG 0.546595 1_1:-65259679 Msa0036670:three_prime_UTR
TCCCTAATTGATGTGTCTAG+AGG 0.558445 1_1:-65262332 Msa0036670:intron
TATTGTTGTTGAAATGTTCA+GGG 0.560397 1_1:-65259575 Msa0036670:three_prime_UTR
GTTCGATCAATGAAAGAATG+TGG 0.571899 1_1:-65260542 Msa0036670:CDS
CGAATCTCCCCAAAACGCTA+AGG 0.572083 1_1:+65259640 None:intergenic
TTTAAAGTCGGCGAAACAGA+AGG 0.572954 1_1:-65260321 Msa0036670:CDS
CTGAACCACCAAGATAAGCC+TGG 0.574521 1_1:-65259706 Msa0036670:exon
CGTGGCTAACGAACCATCTG+CGG 0.588139 1_1:-65260693 Msa0036670:CDS
TTCAAGTGGCAACAGCCTGA+TGG 0.588182 1_1:-65260290 Msa0036670:CDS
AAGATAAATACTGGAAACAG+TGG 0.593609 1_1:-65259765 Msa0036670:CDS
GGTTACTTCTGTTGCAACTG+TGG 0.594160 1_1:+65259522 None:intergenic
CCACGGTCAATCACATCAGA+TGG 0.594213 1_1:-65260218 Msa0036670:CDS
ACTGGAAACAGTGGATTCGA+AGG 0.594257 1_1:-65259756 Msa0036670:CDS
ATAATAACAACCGAAGAGAA+GGG 0.597822 1_1:-65262923 Msa0036670:five_prime_UTR
TCACTGTCGTGTCGTGTGTG+GGG 0.609730 1_1:-65262978 Msa0036670:five_prime_UTR
CACTGTCGTGTCGTGTGTGG+GGG 0.612270 1_1:-65262977 Msa0036670:five_prime_UTR
TACGATATCCTGATTTGCCA+CGG 0.614233 1_1:-65260235 Msa0036670:CDS
TAAGAAGCTTCTCGAGAACG+AGG 0.616733 1_1:-65259790 Msa0036670:CDS
ATCAATGACTAAAACAAGGA+GGG 0.619191 1_1:+65259550 None:intergenic
TATCTCCACAAAACCATCAA+CGG 0.623173 1_1:+65260745 None:intergenic
ATAAAGTAAAGCCCCGCAGA+TGG 0.623723 1_1:+65260680 None:intergenic
AGAAGGTTAACAGTTTCAAG+TGG 0.626891 1_1:-65260304 Msa0036670:CDS
TAATAAAGAAGCTAAACTAG+AGG 0.628039 1_1:-65260048 Msa0036670:CDS
TGTGGAGATAAGTGAATGCA+TGG 0.634348 1_1:-65260732 Msa0036670:CDS
CCGTGATGATTATTGCGACA+CGG 0.638583 1_1:-65260003 Msa0036670:CDS
TTGAGTAACATGTTACAACC+AGG 0.644492 1_1:+65259688 None:intergenic
GACACGGATGAAGAAGAAAG+AGG 0.646339 1_1:-65259987 Msa0036670:intron
GTCTATCAATGACTAAAACA+AGG 0.651849 1_1:+65259546 None:intergenic
AAGAAGCTAAACTAGAGGAG+TGG 0.653782 1_1:-65260043 Msa0036670:CDS
CCGTGTCGCAATAATCATCA+CGG 0.657697 1_1:+65260003 None:intergenic
ATTGTTGTTGAAATGTTCAG+GGG 0.666820 1_1:-65259574 Msa0036670:three_prime_UTR
TATCAATGACTAAAACAAGG+AGG 0.672388 1_1:+65259549 None:intergenic
TGCACCATAAATGTCACATG+TGG 0.672914 1_1:+65259438 None:intergenic
GGCAGAACTTCATCATTACA+GGG 0.676331 1_1:-65260197 Msa0036670:CDS
AACAACAATATCATATAACA+GGG 0.694890 1_1:+65259588 None:intergenic
CCATCTGATGTGATTGACCG+TGG 0.711172 1_1:+65260218 None:intergenic
GGCGGATATGATCAAATACG+TGG 0.717411 1_1:-65260711 Msa0036670:CDS
TGGCTAACGAACCATCTGCG+GGG 0.756577 1_1:-65260691 Msa0036670:CDS
GGAGATAAGTGAATGCATGG+CGG 0.818548 1_1:-65260729 Msa0036670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATAAATAAATAAATAA+AGG + chr1_1:65259647-65259666 None:intergenic 0.0%
!! AATCACATAATTATTATAAA+GGG + chr1_1:65259603-65259622 None:intergenic 10.0%
!! AAATCACATAATTATTATAA+AGG + chr1_1:65259604-65259623 None:intergenic 10.0%
!!! TTTATGTTTGTTAATAAAAA+CGG + chr1_1:65260291-65260310 None:intergenic 10.0%
!! AAAATAAATATTGATAAAGT+AGG - chr1_1:65260712-65260731 Msa0036670:CDS 10.0%
!! ATCACATAATTATTATAAAG+GGG + chr1_1:65259602-65259621 None:intergenic 15.0%
!! TAGTATTTGTAAATATTACA+GGG + chr1_1:65260104-65260123 None:intergenic 15.0%
!! ATAGTATTTGTAAATATTAC+AGG + chr1_1:65260105-65260124 None:intergenic 15.0%
!! AAACTATAATAATATGCTAA+AGG - chr1_1:65260354-65260373 Msa0036670:CDS 15.0%
!! AAAACAAAAAAACCAAAAAA+AGG - chr1_1:65261913-65261932 Msa0036670:intron 15.0%
!! AAACAAAAAAACCAAAAAAA+GGG - chr1_1:65261914-65261933 Msa0036670:intron 15.0%
!!! TTTTTCAAATGTTTTAAAGT+CGG - chr1_1:65262044-65262063 Msa0036670:intron 15.0%
!!! AATAATAAAGTCTGATATTT+TGG + chr1_1:65262995-65263014 None:intergenic 15.0%
!! TCACATAATTATTATAAAGG+GGG + chr1_1:65259601-65259620 None:intergenic 20.0%
!! TAATTATGTGATTTATCAAG+AGG - chr1_1:65259610-65259629 Msa0036670:three_prime_UTR 20.0%
!! AAGGCAAAATAATTTAAGAA+AGG + chr1_1:65259821-65259840 None:intergenic 20.0%
!! AAACATAAAATCTGTATGAA+TGG - chr1_1:65260302-65260321 Msa0036670:CDS 20.0%
!! ATCTTTCCATTTACAAAAAT+GGG + chr1_1:65260828-65260847 None:intergenic 20.0%
!! TATCTTTCCATTTACAAAAA+TGG + chr1_1:65260829-65260848 None:intergenic 20.0%
!!! TTATGATTTGGAATCTTTTT+TGG - chr1_1:65261003-65261022 Msa0036670:intron 20.0%
!!! TATGATTTGGAATCTTTTTT+GGG - chr1_1:65261004-65261023 Msa0036670:intron 20.0%
!!! ATTATATATGATGATGATGA+TGG + chr1_1:65261382-65261401 None:intergenic 20.0%
!! AACAACAATATCATATAACA+GGG + chr1_1:65262792-65262811 None:intergenic 20.0%
!!! ATCTTATCTTCACTTTTTCT+GGG - chr1_1:65259490-65259509 Msa0036670:three_prime_UTR 25.0%
! TTATGCTTTATATCTGAATG+TGG - chr1_1:65259710-65259729 Msa0036670:exon 25.0%
! TATGCTTTATATCTGAATGT+GGG - chr1_1:65259711-65259730 Msa0036670:exon 25.0%
!!! TAATTTAAGAAAGGAGTGTT+TGG + chr1_1:65259812-65259831 None:intergenic 25.0%
!! TAACAAAAATGACTTGGAAA+AGG + chr1_1:65259840-65259859 None:intergenic 25.0%
! GTCAAATAACAAAAATGACT+TGG + chr1_1:65259846-65259865 None:intergenic 25.0%
!! AGATATGATTTTGTTCTTCA+TGG - chr1_1:65259980-65259999 Msa0036670:intron 25.0%
!!! TTTGCTTCCTGATTTTTTAA+AGG - chr1_1:65260018-65260037 Msa0036670:CDS 25.0%
! GTAAATATTACAGGGAATTA+AGG + chr1_1:65260096-65260115 None:intergenic 25.0%
! GGATGAAATCAAAATAATCA+TGG + chr1_1:65260413-65260432 None:intergenic 25.0%
!!! TGATTATTTTGATTTCATCC+CGG - chr1_1:65260413-65260432 Msa0036670:CDS 25.0%
! TCAATGGTAAATGGATAAAA+AGG - chr1_1:65260644-65260663 Msa0036670:CDS 25.0%
! GTAAATGGATAAAAAGGAAA+AGG - chr1_1:65260650-65260669 Msa0036670:CDS 25.0%
! AAATCTATATCTTAGGTGAT+AGG - chr1_1:65260760-65260779 Msa0036670:CDS 25.0%
!!! ATGATTCCCATTTTTGTAAA+TGG - chr1_1:65260819-65260838 Msa0036670:intron 25.0%
! AAATGGAAAGATATTTGTTC+AGG - chr1_1:65260836-65260855 Msa0036670:intron 25.0%
! AATGGAAAGATATTTGTTCA+GGG - chr1_1:65260837-65260856 Msa0036670:intron 25.0%
! AAAAACAGAAACAGCAAATA+AGG + chr1_1:65260923-65260942 None:intergenic 25.0%
!!! AGTTCTTTTAGAACATTCAT+GGG - chr1_1:65260973-65260992 Msa0036670:intron 25.0%
!! GATGGAATTTTAAACTGATA+AGG + chr1_1:65261364-65261383 None:intergenic 25.0%
! GATGAACAAATTCAATTGAA+TGG + chr1_1:65261410-65261429 None:intergenic 25.0%
! AATTGAATGTATCAATCCAA+TGG - chr1_1:65261467-65261486 Msa0036670:intron 25.0%
! TCAACATTTACTGATACAAA+AGG + chr1_1:65261506-65261525 None:intergenic 25.0%
!! TGTATCAGTAAATGTTGATT+AGG - chr1_1:65261508-65261527 Msa0036670:intron 25.0%
! AACTTACTATGTTTCTTTCT+TGG - chr1_1:65261561-65261580 Msa0036670:intron 25.0%
! TGACAAAAATATTGTCGAAA+AGG - chr1_1:65261762-65261781 Msa0036670:intron 25.0%
! TAATAAAGAAGCTAAACTAG+AGG - chr1_1:65262329-65262348 Msa0036670:intron 25.0%
! AACATGTTACTCAATTTCAA+AGG - chr1_1:65262698-65262717 Msa0036670:intron 25.0%
! CAACAACAATATCATATAAC+AGG + chr1_1:65262793-65262812 None:intergenic 25.0%
!! ATATTGTTGTTGAAATGTTC+AGG - chr1_1:65262801-65262820 Msa0036670:intron 25.0%
!! TATTGTTGTTGAAATGTTCA+GGG - chr1_1:65262802-65262821 Msa0036670:intron 25.0%
! ACTTTGCAAATCATATTATC+GGG + chr1_1:65262879-65262898 None:intergenic 25.0%
! CACTTTGCAAATCATATTAT+CGG + chr1_1:65262880-65262899 None:intergenic 25.0%
ATAATAACAACCGAAGAGAA+GGG - chr1_1:65259454-65259473 Msa0036670:three_prime_UTR 30.0%
!!! CATCTTATCTTCACTTTTTC+TGG - chr1_1:65259489-65259508 Msa0036670:three_prime_UTR 30.0%
TTGATTTAAATACCCTCTCA+AGG + chr1_1:65259549-65259568 None:intergenic 30.0%
TGATTTATCAAGAGGAACAA+AGG - chr1_1:65259618-65259637 Msa0036670:three_prime_UTR 30.0%
! GGAAACACCTTTAAAAAATC+AGG + chr1_1:65260028-65260047 None:intergenic 30.0%
CATCAATGTAAGAAAATGTG+AGG - chr1_1:65260176-65260195 Msa0036670:CDS 30.0%
TTGAAATAGTAGCTACTACT+TGG - chr1_1:65260230-65260249 Msa0036670:CDS 30.0%
!!! GATTATTTTGATTTCATCCC+GGG - chr1_1:65260414-65260433 Msa0036670:CDS 30.0%
TTCAAAAAACATTAGGTTCC+CGG + chr1_1:65260435-65260454 None:intergenic 30.0%
!!! GGAACCTAATGTTTTTTGAA+TGG - chr1_1:65260435-65260454 Msa0036670:CDS 30.0%
AGCTCCATTCAAAAAACATT+AGG + chr1_1:65260442-65260461 None:intergenic 30.0%
TTAATACACACACGTGTAAA+AGG + chr1_1:65260540-65260559 None:intergenic 30.0%
AGTCTTACAGGAAAATACAT+TGG + chr1_1:65260581-65260600 None:intergenic 30.0%
! TCTCTGGAAATCTATATCTT+AGG - chr1_1:65260753-65260772 Msa0036670:CDS 30.0%
GCAAATAGGATATGAAAGTA+AGG + chr1_1:65260791-65260810 None:intergenic 30.0%
TTCATATCCTATTTGCCAAT+TGG - chr1_1:65260795-65260814 Msa0036670:intron 30.0%
TCATATCCTATTTGCCAATT+GGG - chr1_1:65260796-65260815 Msa0036670:intron 30.0%
ATGGAAAGATATTTGTTCAG+GGG - chr1_1:65260838-65260857 Msa0036670:intron 30.0%
ACAATTCAGAGAGATGATAA+TGG + chr1_1:65260893-65260912 None:intergenic 30.0%
!! CATGAATGTTCTAAAAGAAC+TGG + chr1_1:65260974-65260993 None:intergenic 30.0%
!!! CAGTTCTTTTAGAACATTCA+TGG - chr1_1:65260972-65260991 Msa0036670:intron 30.0%
!! ATAGGATTTGCTTTTCCAAA+AGG + chr1_1:65261041-65261060 None:intergenic 30.0%
TTGGAAAAGCAAATCCTATT+AGG - chr1_1:65261042-65261061 Msa0036670:intron 30.0%
ATGATTGACTAGACATAAAG+AGG + chr1_1:65261095-65261114 None:intergenic 30.0%
CTAGAGTGATAAAGTTCTTA+TGG - chr1_1:65261219-65261238 Msa0036670:intron 30.0%
! ATAAGGATTTCACGTTTTAG+TGG + chr1_1:65261347-65261366 None:intergenic 30.0%
! GAAACATAGTAAGTTGACTA+GGG + chr1_1:65261556-65261575 None:intergenic 30.0%
! AGAAACATAGTAAGTTGACT+AGG + chr1_1:65261557-65261576 None:intergenic 30.0%
! ATGAACTGTATTTTGTCTTC+AGG - chr1_1:65261590-65261609 Msa0036670:intron 30.0%
! TTGTCAGCTTTAATGACATT+TGG + chr1_1:65261748-65261767 None:intergenic 30.0%
ACATTCTTTCATTGATCGAA+CGG + chr1_1:65261836-65261855 None:intergenic 30.0%
AATGCTGATATCACTTTCAT+TGG + chr1_1:65262268-65262287 None:intergenic 30.0%
AAGATAAATACTGGAAACAG+TGG - chr1_1:65262612-65262631 Msa0036670:intron 30.0%
!! ATTTTAGTCCTTAGCGTTTT+GGG - chr1_1:65262729-65262748 Msa0036670:intron 30.0%
! ATTGTTGTTGAAATGTTCAG+GGG - chr1_1:65262803-65262822 Msa0036670:intron 30.0%
ATCAATGACTAAAACAAGGA+GGG + chr1_1:65262830-65262849 None:intergenic 30.0%
TATCAATGACTAAAACAAGG+AGG + chr1_1:65262831-65262850 None:intergenic 30.0%
GTCTATCAATGACTAAAACA+AGG + chr1_1:65262834-65262853 None:intergenic 30.0%
! TGTGTTTCCTTTTCATGTTT+TGG - chr1_1:65262913-65262932 Msa0036670:five_prime_UTR 30.0%
! CTTCGGTTGTTATTATGGTA+TGG + chr1_1:65259450-65259469 None:intergenic 35.0%
! CTTCTCTTCGGTTGTTATTA+TGG + chr1_1:65259455-65259474 None:intergenic 35.0%
CATAATAACAACCGAAGAGA+AGG - chr1_1:65259453-65259472 Msa0036670:three_prime_UTR 35.0%
AATTTCAGCGATGAAGAAGA+AGG + chr1_1:65259526-65259545 None:intergenic 35.0%
!! TTTAAGAAAGGAGTGTTTGG+TGG + chr1_1:65259809-65259828 None:intergenic 35.0%
TTCTTCATGGACTTTCTTGT+AGG - chr1_1:65259993-65260012 Msa0036670:CDS 35.0%
ACCTCTAGACACATCAATTA+GGG + chr1_1:65260049-65260068 None:intergenic 35.0%
AACCTCTAGACACATCAATT+AGG + chr1_1:65260050-65260069 None:intergenic 35.0%
AGGGAATTAAGGCTAAAAAG+AGG + chr1_1:65260085-65260104 None:intergenic 35.0%
! ACTTGGTAATTCACACTTTG+TGG - chr1_1:65260247-65260266 Msa0036670:CDS 35.0%
! TCAAAATAATCATGGTGCCA+TGG + chr1_1:65260405-65260424 None:intergenic 35.0%
TCAAAAAACATTAGGTTCCC+GGG + chr1_1:65260434-65260453 None:intergenic 35.0%
TTTGAATGGAGCTACTAAGT+TGG - chr1_1:65260449-65260468 Msa0036670:CDS 35.0%
TGTGACTCGAAAAACTCATT+TGG - chr1_1:65260478-65260497 Msa0036670:CDS 35.0%
! GAAAATACATTGGCTGACAA+GGG + chr1_1:65260571-65260590 None:intergenic 35.0%
AGTAGTGTACGTCATTTGTT+CGG - chr1_1:65260618-65260637 Msa0036670:CDS 35.0%
! GATAGTTTCACATTAGTCTC+TGG - chr1_1:65260737-65260756 Msa0036670:CDS 35.0%
AATCATCCCAATTGGCAAAT+AGG + chr1_1:65260805-65260824 None:intergenic 35.0%
AAAATGGGAATCATCCCAAT+TGG + chr1_1:65260813-65260832 None:intergenic 35.0%
GGAAATTCCAAAACAGATTC+AGG + chr1_1:65260953-65260972 None:intergenic 35.0%
ATGGGTCGAGATTTATGATT+TGG - chr1_1:65260991-65261010 Msa0036670:intron 35.0%
AATCTCACACATAAACGATG+CGG - chr1_1:65261125-65261144 Msa0036670:intron 35.0%
!! TCACGTTTTAGTGGAATGAT+TGG + chr1_1:65261338-65261357 None:intergenic 35.0%
AGGCTAATTTAGACAACCAT+TGG + chr1_1:65261486-65261505 None:intergenic 35.0%
TATCTCCACAAAACCATCAA+CGG + chr1_1:65261635-65261654 None:intergenic 35.0%
GTTCGATCAATGAAAGAATG+TGG - chr1_1:65261835-65261854 Msa0036670:intron 35.0%
TCCATAGTCAATGAGATGAT+AGG - chr1_1:65261862-65261881 Msa0036670:intron 35.0%
! ACCTATCATCTCATTGACTA+TGG + chr1_1:65261866-65261885 None:intergenic 35.0%
AGGTGACATAAACAAATCTC+TGG - chr1_1:65261882-65261901 Msa0036670:intron 35.0%
! GTTGATGTTGATGAATGGAT+TGG + chr1_1:65261940-65261959 None:intergenic 35.0%
!! TTGGTGTTGATGTTGATGAA+TGG + chr1_1:65261945-65261964 None:intergenic 35.0%
GTTCTTTCTGTCTGAGAATT+TGG + chr1_1:65261964-65261983 None:intergenic 35.0%
! AGACAGAAAGAACTCGTTTT+TGG - chr1_1:65261971-65261990 Msa0036670:intron 35.0%
!! GACAGAAAGAACTCGTTTTT+GGG - chr1_1:65261972-65261991 Msa0036670:intron 35.0%
!! ACAGAAAGAACTCGTTTTTG+GGG - chr1_1:65261973-65261992 Msa0036670:intron 35.0%
!! CAATCATGCTTTTTATGCTC+AGG - chr1_1:65261996-65262015 Msa0036670:intron 35.0%
!! ATGCTTTTTATGCTCAGGAA+GGG - chr1_1:65262001-65262020 Msa0036670:intron 35.0%
AGAAGGTTAACAGTTTCAAG+TGG - chr1_1:65262073-65262092 Msa0036670:intron 35.0%
TATAGGCAAATCATCAGGTT+CGG + chr1_1:65262208-65262227 None:intergenic 35.0%
AGTTGTTGTCATTGTCAGAA+AGG - chr1_1:65262289-65262308 Msa0036670:intron 35.0%
! GAAGAAGAAAGAGGTTAGTT+TGG - chr1_1:65262399-65262418 Msa0036670:intron 35.0%
GAACAAACATGATACGCTAT+TGG - chr1_1:65262495-65262514 Msa0036670:intron 35.0%
! GTTGATTTCCTTATGTCAAC+AGG - chr1_1:65262564-65262583 Msa0036670:intron 35.0%
AACGAGGAGAAGATAAATAC+TGG - chr1_1:65262603-65262622 Msa0036670:intron 35.0%
TTGAGTAACATGTTACAACC+AGG + chr1_1:65262692-65262711 None:intergenic 35.0%
!! CATTTTAGTCCTTAGCGTTT+TGG - chr1_1:65262728-65262747 Msa0036670:intron 35.0%
! TTTTAGTCCTTAGCGTTTTG+GGG - chr1_1:65262730-65262749 Msa0036670:intron 35.0%
!! GTTTCCTTTTCATGTTTTGG+TGG - chr1_1:65262916-65262935 Msa0036670:five_prime_UTR 35.0%
!!! ATGGTGCACTAGTTTTAAAG+TGG - chr1_1:65262954-65262973 Msa0036670:five_prime_UTR 35.0%
ATCGCTGAAATTCCTTGAGA+GGG - chr1_1:65259534-65259553 Msa0036670:three_prime_UTR 40.0%
TCCCTAATTGATGTGTCTAG+AGG - chr1_1:65260045-65260064 Msa0036670:CDS 40.0%
AAATGTGAGGCAGAGAAATG+TGG - chr1_1:65260189-65260208 Msa0036670:CDS 40.0%
AATGTGAGGCAGAGAAATGT+GGG - chr1_1:65260190-65260209 Msa0036670:CDS 40.0%
TGTATGAATGGAAGAGAGCA+AGG - chr1_1:65260314-65260333 Msa0036670:CDS 40.0%
AATGGAAGAGAGCAAGGATA+AGG - chr1_1:65260320-65260339 Msa0036670:CDS 40.0%
ATGGAAGAGAGCAAGGATAA+GGG - chr1_1:65260321-65260340 Msa0036670:CDS 40.0%
!! AATAATCATGGTGCCATGGT+GGG + chr1_1:65260401-65260420 None:intergenic 40.0%
!! AAATAATCATGGTGCCATGG+TGG + chr1_1:65260402-65260421 None:intergenic 40.0%
! TTTGGCTGTGTTTAAAGTCG+TGG - chr1_1:65260496-65260515 Msa0036670:CDS 40.0%
! GGAAAATACATTGGCTGACA+AGG + chr1_1:65260572-65260591 None:intergenic 40.0%
TCAGTACTCTTCAGTCTTAC+AGG + chr1_1:65260593-65260612 None:intergenic 40.0%
GAACAAATGACGTACACTAC+TGG + chr1_1:65260619-65260638 None:intergenic 40.0%
GTAGTGTACGTCATTTGTTC+GGG - chr1_1:65260619-65260638 Msa0036670:CDS 40.0%
CCAAAACAGATTCAGGACTT+CGG + chr1_1:65260946-65260965 None:intergenic 40.0%
AGCAAATCCTATTAGGTGTG+TGG - chr1_1:65261049-65261068 Msa0036670:intron 40.0%
CTATTGACCACACACCTAAT+AGG + chr1_1:65261059-65261078 None:intergenic 40.0%
!! CGTTTTAGTGGAATGATTGG+AGG + chr1_1:65261335-65261354 None:intergenic 40.0%
CATGAATTCTCTACCGTTGA+TGG - chr1_1:65261619-65261638 Msa0036670:intron 40.0%
TGTGGAGATAAGTGAATGCA+TGG - chr1_1:65261645-65261664 Msa0036670:intron 40.0%
! CTGCATTTTGAGCATGTTGT+TGG + chr1_1:65261723-65261742 None:intergenic 40.0%
AACTTTGCACACTGAAGACT+TGG - chr1_1:65261795-65261814 Msa0036670:intron 40.0%
!!! GAATGGATTGGCCCTTTTTT+TGG + chr1_1:65261928-65261947 None:intergenic 40.0%
!! CATGCTTTTTATGCTCAGGA+AGG - chr1_1:65262000-65262019 Msa0036670:intron 40.0%
!! TGCTTTTTATGCTCAGGAAG+GGG - chr1_1:65262002-65262021 Msa0036670:intron 40.0%
! TTTAAAGTCGGCGAAACAGA+AGG - chr1_1:65262056-65262075 Msa0036670:intron 40.0%
TCTACTGATCCTTTACCATC+AGG + chr1_1:65262105-65262124 None:intergenic 40.0%
ATATCGTAGCTTCTCTTCGT+TGG + chr1_1:65262130-65262149 None:intergenic 40.0%
! TACGATATCCTGATTTGCCA+CGG - chr1_1:65262142-65262161 Msa0036670:intron 40.0%
TGGCAGAACTTCATCATTAC+AGG - chr1_1:65262179-65262198 Msa0036670:intron 40.0%
GGCAGAACTTCATCATTACA+GGG - chr1_1:65262180-65262199 Msa0036670:intron 40.0%
CTCGATATAGGCAAATCATC+AGG + chr1_1:65262213-65262232 None:intergenic 40.0%
! TGATTTGCCTATATCGAGTC+AGG - chr1_1:65262215-65262234 Msa0036670:intron 40.0%
! TCTTCAACCTGACTCGATAT+AGG + chr1_1:65262225-65262244 None:intergenic 40.0%
AAGAAGCTAAACTAGAGGAG+TGG - chr1_1:65262334-65262353 Msa0036670:five_prime_UTR 40.0%
GCTTCTTACCTGTTGACATA+AGG + chr1_1:65262575-65262594 None:intergenic 40.0%
TGTTACAACCAGGCTTATCT+TGG + chr1_1:65262682-65262701 None:intergenic 40.0%
TGCACCATAAATGTCACATG+TGG + chr1_1:65262942-65262961 None:intergenic 40.0%
AGACTCATGACCCTTCTCTT+CGG + chr1_1:65259467-65259486 None:intergenic 45.0%
CATCGCTGAAATTCCTTGAG+AGG - chr1_1:65259533-65259552 Msa0036670:three_prime_UTR 45.0%
! TATCTCTCTAGTTCCCACCA+TGG - chr1_1:65260385-65260404 Msa0036670:CDS 45.0%
GTCATTTGTTCGGGAGTCAA+TGG - chr1_1:65260628-65260647 Msa0036670:CDS 45.0%
GTTCGGGAGTCAATGGTAAA+TGG - chr1_1:65260635-65260654 Msa0036670:CDS 45.0%
!! CAGAGAGATGATAATGGCAG+AGG + chr1_1:65260887-65260906 None:intergenic 45.0%
! CCGAAGTCCTGAATCTGTTT+TGG - chr1_1:65260943-65260962 Msa0036670:intron 45.0%
!! TGGGCTTTGTAGACTCCTTT+TGG - chr1_1:65261023-65261042 Msa0036670:intron 45.0%
!!! CTCTACCGTTGATGGTTTTG+TGG - chr1_1:65261627-65261646 Msa0036670:intron 45.0%
GGAGATAAGTGAATGCATGG+CGG - chr1_1:65261648-65261667 Msa0036670:intron 45.0%
GGCGGATATGATCAAATACG+TGG - chr1_1:65261666-65261685 Msa0036670:intron 45.0%
ATAAAGTAAAGCCCCGCAGA+TGG + chr1_1:65261700-65261719 None:intergenic 45.0%
TTTGCACACTGAAGACTTGG+AGG - chr1_1:65261798-65261817 Msa0036670:intron 45.0%
AAACTAGAGGAGTGGCTTGA+AGG - chr1_1:65262342-65262361 Msa0036670:five_prime_UTR 45.0%
CCGTGTCGCAATAATCATCA+CGG + chr1_1:65262377-65262396 None:intergenic 45.0%
CCGTGATGATTATTGCGACA+CGG - chr1_1:65262374-65262393 Msa0036670:intron 45.0%
GACACGGATGAAGAAGAAAG+AGG - chr1_1:65262390-65262409 Msa0036670:intron 45.0%
TAAGAAGCTTCTCGAGAACG+AGG - chr1_1:65262587-65262606 Msa0036670:intron 45.0%
! ACTGGAAACAGTGGATTCGA+AGG - chr1_1:65262621-65262640 Msa0036670:intron 45.0%
! AGGCTTATCTTGGTGGTTCA+GGG + chr1_1:65262672-65262691 None:intergenic 45.0%
TACAACCAGGCTTATCTTGG+TGG + chr1_1:65262679-65262698 None:intergenic 45.0%
GGTTACTTCTGTTGCAACTG+TGG + chr1_1:65262858-65262877 None:intergenic 45.0%
GTGGCCACCAAAACATGAAA+AGG + chr1_1:65262923-65262942 None:intergenic 45.0%
GTGGCCACATGTGACATTTA+TGG - chr1_1:65262935-65262954 Msa0036670:five_prime_UTR 45.0%
GAGGCAGAGAAATGTGGGTA+TGG - chr1_1:65260195-65260214 Msa0036670:CDS 50.0%
ATAAACGATGCGGCAGCTGT+AGG - chr1_1:65261135-65261154 Msa0036670:intron 50.0%
CATCTGCTGCAATACGCGTT+AGG - chr1_1:65261426-65261445 Msa0036670:intron 50.0%
TTCAAGTGGCAACAGCCTGA+TGG - chr1_1:65262087-65262106 Msa0036670:intron 50.0%
TGGCAACAGCCTGATGGTAA+AGG - chr1_1:65262093-65262112 Msa0036670:intron 50.0%
GTGATTGACCGTGGCAAATC+AGG + chr1_1:65262153-65262172 None:intergenic 50.0%
CCATCTGATGTGATTGACCG+TGG + chr1_1:65262162-65262181 None:intergenic 50.0%
CCACGGTCAATCACATCAGA+TGG - chr1_1:65262159-65262178 Msa0036670:intron 50.0%
! CAGGCTTATCTTGGTGGTTC+AGG + chr1_1:65262673-65262692 None:intergenic 50.0%
CTGAACCACCAAGATAAGCC+TGG - chr1_1:65262671-65262690 Msa0036670:intron 50.0%
CGAATCTCCCCAAAACGCTA+AGG + chr1_1:65262740-65262759 None:intergenic 50.0%
TGTCACTGTCGTGTCGTGTG+TGG - chr1_1:65259397-65259416 Msa0036670:three_prime_UTR 55.0%
GTCACTGTCGTGTCGTGTGT+GGG - chr1_1:65259398-65259417 Msa0036670:three_prime_UTR 55.0%
TCACTGTCGTGTCGTGTGTG+GGG - chr1_1:65259399-65259418 Msa0036670:three_prime_UTR 55.0%
GTGTGTGGGGGACTGTTCTT+TGG - chr1_1:65259412-65259431 Msa0036670:three_prime_UTR 55.0%
TGGGGGACTGTTCTTTGGTG+TGG - chr1_1:65259417-65259436 Msa0036670:three_prime_UTR 55.0%
CGTGGCTAACGAACCATCTG+CGG - chr1_1:65261684-65261703 Msa0036670:intron 55.0%
GTGGCTAACGAACCATCTGC+GGG - chr1_1:65261685-65261704 Msa0036670:intron 55.0%
TGGCTAACGAACCATCTGCG+GGG - chr1_1:65261686-65261705 Msa0036670:intron 55.0%
CACTGTCGTGTCGTGTGTGG+GGG - chr1_1:65259400-65259419 Msa0036670:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 65259370 65263029 65259370 ID=Msa0036670;Name=Msa0036670
chr1_1 mRNA 65259370 65263029 65259370 ID=Msa0036670-mRNA-1;Parent=Msa0036670;Name=Msa0036670-mRNA-1;_AED=0.05;_eAED=0.05;_QI=198|1|1|1|0.33|0.25|4|355|294
chr1_1 exon 65259370 65259813 65259370 ID=Msa0036670-mRNA-1:exon:21011;Parent=Msa0036670-mRNA-1
chr1_1 exon 65259988 65260787 65259988 ID=Msa0036670-mRNA-1:exon:21010;Parent=Msa0036670-mRNA-1
chr1_1 exon 65262333 65262384 65262333 ID=Msa0036670-mRNA-1:exon:21009;Parent=Msa0036670-mRNA-1
chr1_1 exon 65262888 65263029 65262888 ID=Msa0036670-mRNA-1:exon:21008;Parent=Msa0036670-mRNA-1
chr1_1 five_prime_UTR 65262888 65263029 65262888 ID=Msa0036670-mRNA-1:five_prime_utr;Parent=Msa0036670-mRNA-1
chr1_1 five_prime_UTR 65262333 65262384 65262333 ID=Msa0036670-mRNA-1:five_prime_utr;Parent=Msa0036670-mRNA-1
chr1_1 five_prime_UTR 65260784 65260787 65260784 ID=Msa0036670-mRNA-1:five_prime_utr;Parent=Msa0036670-mRNA-1
chr1_1 CDS 65259988 65260783 65259988 ID=Msa0036670-mRNA-1:cds;Parent=Msa0036670-mRNA-1
chr1_1 CDS 65259725 65259813 65259725 ID=Msa0036670-mRNA-1:cds;Parent=Msa0036670-mRNA-1
chr1_1 three_prime_UTR 65259370 65259724 65259370 ID=Msa0036670-mRNA-1:three_prime_utr;Parent=Msa0036670-mRNA-1
Gene Sequence

>Msa0036670

ATGCATGAATTCTCTACCGTTGATGGTTTTGTGGAGATAAGTGAATGCATGGCGGATATGATCAAATACGTGGCTAACGAACCATCTGCGGGGCTTTACTTTATCCAACAACATGCTCAAAATGCAGTGCCAAATGTCATTAAAGCTGACAAAAATATTGTCGAAAAGGTTCATGAAACAACTTTGCACACTGAAGACTTGGAGGACTCTATTACAGCCGTTCGATCAATGAAAGAATGTGGATTTTCCATAGTCAATGAGATGATAGGTGACATAAACAAATCTCTGGAAATTTTGAAAACAAAAAAACCAAAAAAAGGGCCAATCCATTCATCAACATCAACACCAAATTCTCAGACAGAAAGAACTCGTTTTTGGGGCAATCATGCTTTTTATGCTCAGGAAGGGGATGATAAAAAGAGTAACTATTTTTCAAATGTTTTAAAGTCGGCGAAACAGAAGGTTAACAGTTTCAAGTGGCAACAGCCTGATGGTAAAGGATCAGTAGATTCCAACGAAGAGAAGCTACGATATCCTGATTTGCCACGGTCAATCACATCAGATGGCAGAACTTCATCATTACAGGGAACCGAACCTGATGATTTGCCTATATCGAGTCAGGTTGAAGACGAGTCTCAACACGAACAGACCAATGAAAGTGATATCAGCATTAAGTTGTTGTCATTGTCAGAAAGGTTTGATGACTTTAAAGCTAATAAAGAAGCTAAACTAGAGGAGTGGCTTGAAGGAACTAGCAACCGTGATGATTATTGCGACACGGATGAAGAAGAAAGAGGTAAGAAGCTTCTCGAGAACGAGGAGAAGATAAATACTGGAAACAGTGGATTCGAAGGTGAAGTACTATTGCATGCAGTTCAGCCCTGA
Protein sequence

>Msa0036670

MHEFSTVDGFVEISECMADMIKYVANEPSAGLYFIQQHAQNAVPNVIKADKNIVEKVHETTLHTEDLEDSITAVRSMKECGFSIVNEMIGDINKSLEILKTKKPKKGPIHSSTSTPNSQTERTRFWGNHAFYAQEGDDKKSNYFSNVLKSAKQKVNSFKWQQPDGKGSVDSNEEKLRYPDLPRSITSDGRTSSLQGTEPDDLPISSQVEDESQHEQTNESDISIKLLSLSERFDDFKANKEAKLEEWLEGTSNRDDYCDTDEEERGKKLLENEEKINTGNSGFEGEVLLHAVQP*