AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0075730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0075730 MtrunA17_Chr1g0184681 99.342 152 1 0 1 152 1 152 2.95e-109 307
Msa0075730 MtrunA17_Chr4g0038481 96.711 152 5 0 1 152 1 152 8.07e-105 296
Msa0075730 MtrunA17_Chr8g0391601 96.104 154 4 1 1 152 1 154 1.61e-104 295
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0075730 AT5G09510.1 94.079 152 9 0 1 152 1 152 6.82e-96 273
Msa0075730 AT1G04270.1 94.079 152 9 0 1 152 1 152 1.06e-95 273
Msa0075730 AT5G09500.1 88.816 152 15 1 1 152 1 150 9.89e-95 270
Msa0075730 AT1G04270.2 93.421 152 9 1 1 152 1 151 3.95e-93 266
Msa0075730 AT5G09490.1 82.237 152 27 0 1 152 1 152 1.17e-90 260
Msa0075730 AT5G43640.1 87.919 149 18 0 4 152 1 149 4.43e-85 246
Msa0075730 AT5G09510.2 94.915 118 6 0 35 152 1 118 4.22e-71 209
Msa0075730 AT5G63070.1 62.500 160 52 3 1 152 1 160 3.97e-63 191
Msa0075730 AT1G33850.1 80.597 67 13 0 4 70 1 67 2.53e-22 84.7

Find 71 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTAAATGTTCTCTTCTT+TGG 0.218192 1_2:-51274418 None:intergenic
TGTTGATCTCGATGCTCTTT+TGG 0.301311 1_2:+51274456 Msa0075730:CDS
TTCCCAGCATATCTTTCATT+TGG 0.318679 1_2:-51276320 None:intergenic
AGGTGAGTCCTCACAGGTTC+TGG 0.319193 1_2:-51275774 None:intergenic
ATATCAGTGAACTAAAGTTT+TGG 0.345093 1_2:+51276103 Msa0075730:three_prime_UTR
GAGATGATCGGTAGCATTAT+TGG 0.345545 1_2:+51275819 Msa0075730:CDS
CCGGGCCTACCGTGCTTAAC+AGG 0.350740 1_2:-51275930 None:intergenic
AGATAGTGGCCAATCATTTC+AGG 0.354585 1_2:-51275882 None:intergenic
GATGAGTGCCATAGGCTTGC+GGG 0.362183 1_2:-51275000 None:intergenic
TGAAATGATTGGCCACTATC+TGG 0.368994 1_2:+51275884 Msa0075730:CDS
TCTCTGCTCGTGCACGTAGA+AGG 0.369916 1_2:+51274509 Msa0075730:CDS
ACTTAAATCCTGAGCGAATT+CGG 0.375078 1_2:+51276248 Msa0075730:three_prime_UTR
TATTTGGTTTATAGATAGAT+TGG 0.376185 1_2:+51276178 Msa0075730:three_prime_UTR
GAAATTAAGCCTGAAATGAT+TGG 0.379557 1_2:+51275873 Msa0075730:CDS
CTTAATTTCAACTTGGTTGA+AGG 0.392478 1_2:-51275859 None:intergenic
TACCTCTCAGGGATTGTGCT+AGG 0.395694 1_2:-51274214 None:intergenic
GCGGCTGCTTCTTACCTCTC+AGG 0.399037 1_2:-51274226 None:intergenic
ACAGAAAAGGGAAGCACCTC+CGG 0.413302 1_2:+51275743 Msa0075730:intron
ACTCCTTGTGCAGCTACCTC+TGG 0.435684 1_2:-51274394 None:intergenic
GCAGTGAAACTTGCTCGATC+TGG 0.436867 1_2:-51274258 None:intergenic
AGTCCAAATGAAAGATATGC+TGG 0.445961 1_2:+51276317 Msa0075730:three_prime_UTR
CGGCTGCTTCTTACCTCTCA+GGG 0.459104 1_2:-51274225 None:intergenic
ACCTCTCAGGGATTGTGCTA+GGG 0.474600 1_2:-51274213 None:intergenic
TATTATTGACCATGAAAGCT+TGG 0.476757 1_2:+51276202 Msa0075730:three_prime_UTR
CTTGTCTACCGAATTCGCTC+AGG 0.483917 1_2:-51276256 None:intergenic
TTGAAAATTCAGCCAGATAG+TGG 0.486704 1_2:-51275896 None:intergenic
TTGGTGCTACTCACTCCTCC+AGG 0.496949 1_2:+51275955 Msa0075730:CDS
TGGTTTGACCCGCAAGCCTA+TGG 0.497772 1_2:+51274992 Msa0075730:CDS
GGAGTGAGTAGCACCAATAC+CGG 0.503041 1_2:-51275949 None:intergenic
AGCGAATTCGGTAGACAAGT+TGG 0.504139 1_2:+51276260 Msa0075730:three_prime_UTR
AAGCACGGTAGGCCCGGTAT+TGG 0.508994 1_2:+51275936 Msa0075730:CDS
AATGACAATCTTCACTTCAA+AGG 0.512033 1_2:-51275984 None:intergenic
CCTGTTAAGCACGGTAGGCC+CGG 0.514282 1_2:+51275930 Msa0075730:CDS
CAGAAAAGGGAAGCACCTCC+GGG 0.515716 1_2:+51275744 Msa0075730:intron
ACAGGTTCTGGCTTCTCACC+CGG 0.516641 1_2:-51275762 None:intergenic
TGATGAGTGCCATAGGCTTG+CGG 0.517926 1_2:-51275001 None:intergenic
AGCAAGTTTCACTGCAATCA+TGG 0.522868 1_2:+51274266 Msa0075730:exon
GTTTGCATTGAAAGATAATG+TGG 0.529819 1_2:-51276013 None:intergenic
ATAATGCTACCGATCATCTC+AGG 0.534264 1_2:-51275816 None:intergenic
CAGGCACAATGATCATGTTA+CGG 0.535956 1_2:-51275797 None:intergenic
TTTCAGGCTTAATTTCAACT+TGG 0.556240 1_2:-51275866 None:intergenic
TTTGCATTGAAAGATAATGT+GGG 0.577878 1_2:-51276012 None:intergenic
AGCATTATTGGTGTCTACAA+TGG 0.579267 1_2:+51275831 Msa0075730:CDS
TTTAAGAAGTTCAGTTTCCG+TGG 0.586451 1_2:+51274433 Msa0075730:CDS
TCACTTCAAAGGAATAAACC+TGG 0.587310 1_2:-51275973 None:intergenic
TTACGGAGGTGAGTCCTCAC+AGG 0.595096 1_2:-51275780 None:intergenic
AGCTTCTTGATGAGTGCCAT+AGG 0.595409 1_2:-51275008 None:intergenic
ATAAACCAAATAAAATCAAG+AGG 0.595454 1_2:-51276167 None:intergenic
GTCCAAATGAAAGATATGCT+GGG 0.595723 1_2:+51276318 Msa0075730:three_prime_UTR
TGTATATATTCATGAACTAG+AGG 0.599285 1_2:+51276285 Msa0075730:three_prime_UTR
GAACCAGAGGTAGCTGCACA+AGG 0.605663 1_2:+51274391 Msa0075730:CDS
CTCGATCTGGTTCTTGCTCG+CGG 0.612106 1_2:-51274245 None:intergenic
GAGTGAGTAGCACCAATACC+GGG 0.613332 1_2:-51275948 None:intergenic
TGATATTGTTATGACACTAG+AGG 0.627040 1_2:+51276080 Msa0075730:three_prime_UTR
AAGCTTGACAAGTTCATCAG+TGG 0.629931 1_2:-51274485 None:intergenic
ACAAGCCTGTTAAGCACGGT+AGG 0.632440 1_2:+51275925 Msa0075730:CDS
TTGCATTGAAAGATAATGTG+GGG 0.633934 1_2:-51276011 None:intergenic
TCTTACAAGCCTGTTAAGCA+CGG 0.639372 1_2:+51275921 Msa0075730:CDS
TCAGGCGGATGTTGAACCAG+AGG 0.645787 1_2:+51274378 Msa0075730:intron
ACTCATCAAGAAGCTCCGCA+AGG 0.648877 1_2:+51275016 Msa0075730:CDS
ATCATTGTGCCTGAGATGAT+CGG 0.650070 1_2:+51275807 Msa0075730:CDS
GGTTCTGGCTTCTCACCCGG+AGG 0.654327 1_2:-51275759 None:intergenic
ACAGATAACTCACCGCCTTG+CGG 0.662337 1_2:-51275031 None:intergenic
TCCTTCACAATCCACAACAG+AGG 0.683089 1_2:+51274182 None:intergenic
GTGAGAAGCCAGAACCTGTG+AGG 0.700359 1_2:+51275766 Msa0075730:CDS
ACCCTAGCACAATCCCTGAG+AGG 0.707194 1_2:+51274212 Msa0075730:five_prime_UTR
GCACAATGATCATGTTACGG+AGG 0.711118 1_2:-51275794 None:intergenic
CTTCAAAGGAATAAACCTGG+AGG 0.714921 1_2:-51275970 None:intergenic
CATCAAGAAGCTCCGCAAGG+CGG 0.735053 1_2:+51275019 Msa0075730:CDS
GAGCATCGAGATCAACACCA+CGG 0.739088 1_2:-51274450 None:intergenic
GTTCATTTCAGGTTTCAACG+TGG 0.745899 1_2:+51274972 Msa0075730:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCATTTTGTTTTATTTTG+TGG + chr1_2:51274754-51274773 Msa0075730:intron 15.0%
!!! TTCATTTTGTTTTATTTTGT+GGG + chr1_2:51274755-51274774 Msa0075730:intron 15.0%
!!! TTTTATATTCTGTTACTTAT+AGG + chr1_2:51274880-51274899 Msa0075730:intron 15.0%
!!! TTTCATTTTGTTTTATTTTG+TGG + chr1_2:51274754-51274773 Msa0075730:intron 15.0%
!!! TTCATTTTGTTTTATTTTGT+GGG + chr1_2:51274755-51274774 Msa0075730:intron 15.0%
!!! TTTTATATTCTGTTACTTAT+AGG + chr1_2:51274880-51274899 Msa0075730:intron 15.0%
!!! ATGAATTTGATTGATTTTTC+AGG + chr1_2:51274360-51274379 Msa0075730:intron 20.0%
!! TAACCTAAAAATGATGAAAA+TGG - chr1_2:51274700-51274719 None:intergenic 20.0%
!!! TTCATCATTTTTAGGTTATT+TGG + chr1_2:51274702-51274721 Msa0075730:intron 20.0%
!! AACCTAAAATATTCAACATT+TGG - chr1_2:51274728-51274747 None:intergenic 20.0%
!! TTTAACAATATGTTCATTTC+AGG + chr1_2:51274961-51274980 Msa0075730:intron 20.0%
!! TTTGGAAATTGTTTATTTAG+CGG + chr1_2:51275168-51275187 Msa0075730:intron 20.0%
!! ACAATATACTGTCTTAAATT+TGG + chr1_2:51275391-51275410 Msa0075730:intron 20.0%
!! CAATATACTGTCTTAAATTT+GGG + chr1_2:51275392-51275411 Msa0075730:intron 20.0%
!! AATATACTGTCTTAAATTTG+GGG + chr1_2:51275393-51275412 Msa0075730:intron 20.0%
!!! TTTAAAATGATAGCATGATT+GGG - chr1_2:51275646-51275665 None:intergenic 20.0%
!!! ATTTAAAATGATAGCATGAT+TGG - chr1_2:51275647-51275666 None:intergenic 20.0%
!! TTGTATATTGTTACAGAAAA+GGG + chr1_2:51275731-51275750 Msa0075730:intron 20.0%
!! ATAAACCAAATAAAATCAAG+AGG - chr1_2:51276170-51276189 None:intergenic 20.0%
!!! TATTTGGTTTATAGATAGAT+TGG + chr1_2:51276178-51276197 Msa0075730:three_prime_UTR 20.0%
!!! ATGAATTTGATTGATTTTTC+AGG + chr1_2:51274360-51274379 Msa0075730:intron 20.0%
!! TAACCTAAAAATGATGAAAA+TGG - chr1_2:51274700-51274719 None:intergenic 20.0%
!!! TTCATCATTTTTAGGTTATT+TGG + chr1_2:51274702-51274721 Msa0075730:intron 20.0%
!! AACCTAAAATATTCAACATT+TGG - chr1_2:51274728-51274747 None:intergenic 20.0%
!! TTTAACAATATGTTCATTTC+AGG + chr1_2:51274961-51274980 Msa0075730:intron 20.0%
!! TTTGGAAATTGTTTATTTAG+CGG + chr1_2:51275168-51275187 Msa0075730:intron 20.0%
!! ACAATATACTGTCTTAAATT+TGG + chr1_2:51275391-51275410 Msa0075730:intron 20.0%
!! CAATATACTGTCTTAAATTT+GGG + chr1_2:51275392-51275411 Msa0075730:intron 20.0%
!! AATATACTGTCTTAAATTTG+GGG + chr1_2:51275393-51275412 Msa0075730:intron 20.0%
!!! TTTAAAATGATAGCATGATT+GGG - chr1_2:51275646-51275665 None:intergenic 20.0%
!!! ATTTAAAATGATAGCATGAT+TGG - chr1_2:51275647-51275666 None:intergenic 20.0%
!! TTGTATATTGTTACAGAAAA+GGG + chr1_2:51275731-51275750 Msa0075730:intron 20.0%
!! ATAAACCAAATAAAATCAAG+AGG - chr1_2:51276170-51276189 None:intergenic 20.0%
!!! TATTTGGTTTATAGATAGAT+TGG + chr1_2:51276178-51276197 Msa0075730:three_prime_UTR 20.0%
!!! AATTTGATTGATTTTTCAGG+CGG + chr1_2:51274363-51274382 Msa0075730:intron 25.0%
! TTCTTAAATGTTCTCTTCTT+TGG - chr1_2:51274421-51274440 None:intergenic 25.0%
! GAATATATGAATGATGAAGA+TGG + chr1_2:51274590-51274609 Msa0075730:intron 25.0%
!!! CGTTAATCTGATTTATTTTG+AGG + chr1_2:51274640-51274659 Msa0075730:intron 25.0%
! GAAATTAACACAAATCCTTA+AGG - chr1_2:51274908-51274927 None:intergenic 25.0%
! GTAAATGTGAAAATGAGAAA+TGG - chr1_2:51274940-51274959 None:intergenic 25.0%
!! ATGTAATTGATTCATTTCAG+TGG + chr1_2:51275089-51275108 Msa0075730:intron 25.0%
!! ATTCTAGTTGTTGAGTAATT+TGG + chr1_2:51275150-51275169 Msa0075730:intron 25.0%
! CGTTAATCTGATTTATTATG+AGG + chr1_2:51275291-51275310 Msa0075730:intron 25.0%
! AAAGTAATGAATGGATATGA+AGG - chr1_2:51274670-51274689 None:intergenic 25.0%
!!! ATGTTTTAGATCAAGTAGAA+GGG - chr1_2:51275351-51275370 None:intergenic 25.0%
! ATATACTGTCTTAAATTTGG+GGG + chr1_2:51275394-51275413 Msa0075730:intron 25.0%
! TGTATGATTATGTATCTAAC+TGG + chr1_2:51275543-51275562 Msa0075730:intron 25.0%
!!! ATTTTGGGTACATAATCATA+CGG - chr1_2:51275682-51275701 None:intergenic 25.0%
!! AACACCTAACAACAAATTTT+GGG - chr1_2:51275697-51275716 None:intergenic 25.0%
! GTTGTATATTGTTACAGAAA+AGG + chr1_2:51275730-51275749 Msa0075730:intron 25.0%
! TTTGCATTGAAAGATAATGT+GGG - chr1_2:51276015-51276034 None:intergenic 25.0%
!! ATATCAGTGAACTAAAGTTT+TGG + chr1_2:51276103-51276122 Msa0075730:three_prime_UTR 25.0%
!! TATCAGTGAACTAAAGTTTT+GGG + chr1_2:51276104-51276123 Msa0075730:three_prime_UTR 25.0%
!! TGTTTCCTCTTGATTTTATT+TGG + chr1_2:51276162-51276181 Msa0075730:three_prime_UTR 25.0%
!!! TAATTTTTTCCAAGCTTTCA+TGG - chr1_2:51276214-51276233 None:intergenic 25.0%
! AAGCTTGGAAAAAATTATTG+TGG + chr1_2:51276217-51276236 Msa0075730:three_prime_UTR 25.0%
! AGCTTGGAAAAAATTATTGT+GGG + chr1_2:51276218-51276237 Msa0075730:three_prime_UTR 25.0%
! TGTATATATTCATGAACTAG+AGG + chr1_2:51276285-51276304 Msa0075730:three_prime_UTR 25.0%
!!! AATTTGATTGATTTTTCAGG+CGG + chr1_2:51274363-51274382 Msa0075730:intron 25.0%
! TTCTTAAATGTTCTCTTCTT+TGG - chr1_2:51274421-51274440 None:intergenic 25.0%
! GAATATATGAATGATGAAGA+TGG + chr1_2:51274590-51274609 Msa0075730:intron 25.0%
!!! CGTTAATCTGATTTATTTTG+AGG + chr1_2:51274640-51274659 Msa0075730:intron 25.0%
! GAAATTAACACAAATCCTTA+AGG - chr1_2:51274908-51274927 None:intergenic 25.0%
! GTAAATGTGAAAATGAGAAA+TGG - chr1_2:51274940-51274959 None:intergenic 25.0%
!! ATGTAATTGATTCATTTCAG+TGG + chr1_2:51275089-51275108 Msa0075730:intron 25.0%
!! ATTCTAGTTGTTGAGTAATT+TGG + chr1_2:51275150-51275169 Msa0075730:intron 25.0%
! CGTTAATCTGATTTATTATG+AGG + chr1_2:51275291-51275310 Msa0075730:intron 25.0%
! AAAGTAATGAATGGATATGA+AGG - chr1_2:51274670-51274689 None:intergenic 25.0%
!!! ATGTTTTAGATCAAGTAGAA+GGG - chr1_2:51275351-51275370 None:intergenic 25.0%
! ATATACTGTCTTAAATTTGG+GGG + chr1_2:51275394-51275413 Msa0075730:intron 25.0%
! TGTATGATTATGTATCTAAC+TGG + chr1_2:51275543-51275562 Msa0075730:intron 25.0%
!!! ATTTTGGGTACATAATCATA+CGG - chr1_2:51275682-51275701 None:intergenic 25.0%
!! AACACCTAACAACAAATTTT+GGG - chr1_2:51275697-51275716 None:intergenic 25.0%
! GTTGTATATTGTTACAGAAA+AGG + chr1_2:51275730-51275749 Msa0075730:intron 25.0%
! TTTGCATTGAAAGATAATGT+GGG - chr1_2:51276015-51276034 None:intergenic 25.0%
!! ATATCAGTGAACTAAAGTTT+TGG + chr1_2:51276103-51276122 Msa0075730:three_prime_UTR 25.0%
!! TATCAGTGAACTAAAGTTTT+GGG + chr1_2:51276104-51276123 Msa0075730:three_prime_UTR 25.0%
!! TGTTTCCTCTTGATTTTATT+TGG + chr1_2:51276162-51276181 Msa0075730:three_prime_UTR 25.0%
!!! TAATTTTTTCCAAGCTTTCA+TGG - chr1_2:51276214-51276233 None:intergenic 25.0%
! AAGCTTGGAAAAAATTATTG+TGG + chr1_2:51276217-51276236 Msa0075730:three_prime_UTR 25.0%
! AGCTTGGAAAAAATTATTGT+GGG + chr1_2:51276218-51276237 Msa0075730:three_prime_UTR 25.0%
! TGTATATATTCATGAACTAG+AGG + chr1_2:51276285-51276304 Msa0075730:three_prime_UTR 25.0%
AAGAGAAAGAGATAGAGTTA+GGG - chr1_2:51274298-51274317 None:intergenic 30.0%
!! GCACCATTTTCATCATTTTT+AGG + chr1_2:51274694-51274713 Msa0075730:intron 30.0%
! GGCCAAATGTTGAATATTTT+AGG + chr1_2:51274723-51274742 Msa0075730:intron 30.0%
!! TAGCATTTTGAAGAAATGTG+AGG + chr1_2:51274807-51274826 Msa0075730:intron 30.0%
! TGTTACTTATAGGATCCTTA+AGG + chr1_2:51274890-51274909 Msa0075730:intron 30.0%
GAAATTGTTTATTTAGCGGT+TGG + chr1_2:51275172-51275191 Msa0075730:intron 30.0%
! TCTTTTGCGTTTGTATCTTT+TGG + chr1_2:51275240-51275259 Msa0075730:intron 30.0%
! CTTTTGCGTTTGTATCTTTT+GGG + chr1_2:51275241-51275260 Msa0075730:intron 30.0%
!! GATGTTTTAGATCAAGTAGA+AGG - chr1_2:51275352-51275371 None:intergenic 30.0%
TATACTGTCTTAAATTTGGG+GGG + chr1_2:51275395-51275414 Msa0075730:intron 30.0%
GCATTTGAACTTAACAGATA+TGG + chr1_2:51275421-51275440 Msa0075730:intron 30.0%
ATCTCCTTTACAGATACATT+CGG + chr1_2:51275452-51275471 Msa0075730:intron 30.0%
!! TGTACCCAAAATTTGTTGTT+AGG + chr1_2:51275690-51275709 Msa0075730:intron 30.0%
! GAACACCTAACAACAAATTT+TGG - chr1_2:51275698-51275717 None:intergenic 30.0%
CTTAATTTCAACTTGGTTGA+AGG - chr1_2:51275862-51275881 None:intergenic 30.0%
TTTCAGGCTTAATTTCAACT+TGG - chr1_2:51275869-51275888 None:intergenic 30.0%
GAAATTAAGCCTGAAATGAT+TGG + chr1_2:51275873-51275892 Msa0075730:CDS 30.0%
AATGACAATCTTCACTTCAA+AGG - chr1_2:51275987-51276006 None:intergenic 30.0%
TTGCATTGAAAGATAATGTG+GGG - chr1_2:51276014-51276033 None:intergenic 30.0%
GTTTGCATTGAAAGATAATG+TGG - chr1_2:51276016-51276035 None:intergenic 30.0%
!!! CAAACTTTTGCTTGTTTTAC+TGG + chr1_2:51276031-51276050 Msa0075730:three_prime_UTR 30.0%
TGATATTGTTATGACACTAG+AGG + chr1_2:51276080-51276099 Msa0075730:three_prime_UTR 30.0%
! TTGTCTATTGATGAGCTTTT+TGG + chr1_2:51276133-51276152 Msa0075730:three_prime_UTR 30.0%
TATTATTGACCATGAAAGCT+TGG + chr1_2:51276202-51276221 Msa0075730:three_prime_UTR 30.0%
AAGAGAAAGAGATAGAGTTA+GGG - chr1_2:51274298-51274317 None:intergenic 30.0%
!! GCACCATTTTCATCATTTTT+AGG + chr1_2:51274694-51274713 Msa0075730:intron 30.0%
! GGCCAAATGTTGAATATTTT+AGG + chr1_2:51274723-51274742 Msa0075730:intron 30.0%
!! TAGCATTTTGAAGAAATGTG+AGG + chr1_2:51274807-51274826 Msa0075730:intron 30.0%
! TGTTACTTATAGGATCCTTA+AGG + chr1_2:51274890-51274909 Msa0075730:intron 30.0%
GAAATTGTTTATTTAGCGGT+TGG + chr1_2:51275172-51275191 Msa0075730:intron 30.0%
! TCTTTTGCGTTTGTATCTTT+TGG + chr1_2:51275240-51275259 Msa0075730:intron 30.0%
! CTTTTGCGTTTGTATCTTTT+GGG + chr1_2:51275241-51275260 Msa0075730:intron 30.0%
!! GATGTTTTAGATCAAGTAGA+AGG - chr1_2:51275352-51275371 None:intergenic 30.0%
TATACTGTCTTAAATTTGGG+GGG + chr1_2:51275395-51275414 Msa0075730:intron 30.0%
GCATTTGAACTTAACAGATA+TGG + chr1_2:51275421-51275440 Msa0075730:intron 30.0%
ATCTCCTTTACAGATACATT+CGG + chr1_2:51275452-51275471 Msa0075730:intron 30.0%
!! TGTACCCAAAATTTGTTGTT+AGG + chr1_2:51275690-51275709 Msa0075730:intron 30.0%
! GAACACCTAACAACAAATTT+TGG - chr1_2:51275698-51275717 None:intergenic 30.0%
CTTAATTTCAACTTGGTTGA+AGG - chr1_2:51275862-51275881 None:intergenic 30.0%
TTTCAGGCTTAATTTCAACT+TGG - chr1_2:51275869-51275888 None:intergenic 30.0%
GAAATTAAGCCTGAAATGAT+TGG + chr1_2:51275873-51275892 Msa0075730:CDS 30.0%
AATGACAATCTTCACTTCAA+AGG - chr1_2:51275987-51276006 None:intergenic 30.0%
TTGCATTGAAAGATAATGTG+GGG - chr1_2:51276014-51276033 None:intergenic 30.0%
GTTTGCATTGAAAGATAATG+TGG - chr1_2:51276016-51276035 None:intergenic 30.0%
!!! CAAACTTTTGCTTGTTTTAC+TGG + chr1_2:51276031-51276050 Msa0075730:three_prime_UTR 30.0%
TGATATTGTTATGACACTAG+AGG + chr1_2:51276080-51276099 Msa0075730:three_prime_UTR 30.0%
! TTGTCTATTGATGAGCTTTT+TGG + chr1_2:51276133-51276152 Msa0075730:three_prime_UTR 30.0%
TATTATTGACCATGAAAGCT+TGG + chr1_2:51276202-51276221 Msa0075730:three_prime_UTR 30.0%
GAAGAGAAAGAGATAGAGTT+AGG - chr1_2:51274299-51274318 None:intergenic 35.0%
TTTAAGAAGTTCAGTTTCCG+TGG + chr1_2:51274433-51274452 Msa0075730:CDS 35.0%
TGTATCTAACTGGTTGTGTT+AGG + chr1_2:51275553-51275572 Msa0075730:intron 35.0%
! AGCATTATTGGTGTCTACAA+TGG + chr1_2:51275831-51275850 Msa0075730:CDS 35.0%
TTGAAAATTCAGCCAGATAG+TGG - chr1_2:51275899-51275918 None:intergenic 35.0%
TCACTTCAAAGGAATAAACC+TGG - chr1_2:51275976-51275995 None:intergenic 35.0%
ACTTAAATCCTGAGCGAATT+CGG + chr1_2:51276248-51276267 Msa0075730:three_prime_UTR 35.0%
AGTCCAAATGAAAGATATGC+TGG + chr1_2:51276317-51276336 Msa0075730:three_prime_UTR 35.0%
GTCCAAATGAAAGATATGCT+GGG + chr1_2:51276318-51276337 Msa0075730:three_prime_UTR 35.0%
TTCCCAGCATATCTTTCATT+TGG - chr1_2:51276323-51276342 None:intergenic 35.0%
GAAGAGAAAGAGATAGAGTT+AGG - chr1_2:51274299-51274318 None:intergenic 35.0%
TTTAAGAAGTTCAGTTTCCG+TGG + chr1_2:51274433-51274452 Msa0075730:CDS 35.0%
TGTATCTAACTGGTTGTGTT+AGG + chr1_2:51275553-51275572 Msa0075730:intron 35.0%
! AGCATTATTGGTGTCTACAA+TGG + chr1_2:51275831-51275850 Msa0075730:CDS 35.0%
TTGAAAATTCAGCCAGATAG+TGG - chr1_2:51275899-51275918 None:intergenic 35.0%
TCACTTCAAAGGAATAAACC+TGG - chr1_2:51275976-51275995 None:intergenic 35.0%
ACTTAAATCCTGAGCGAATT+CGG + chr1_2:51276248-51276267 Msa0075730:three_prime_UTR 35.0%
AGTCCAAATGAAAGATATGC+TGG + chr1_2:51276317-51276336 Msa0075730:three_prime_UTR 35.0%
GTCCAAATGAAAGATATGCT+GGG + chr1_2:51276318-51276337 Msa0075730:three_prime_UTR 35.0%
TTCCCAGCATATCTTTCATT+TGG - chr1_2:51276323-51276342 None:intergenic 35.0%
AGCAAGTTTCACTGCAATCA+TGG + chr1_2:51274266-51274285 Msa0075730:exon 40.0%
! TGTTGATCTCGATGCTCTTT+TGG + chr1_2:51274456-51274475 Msa0075730:CDS 40.0%
AAGCTTGACAAGTTCATCAG+TGG - chr1_2:51274488-51274507 None:intergenic 40.0%
GGTGCTCAGAAAGTAATGAA+TGG - chr1_2:51274679-51274698 None:intergenic 40.0%
!! GTTCATTTCAGGTTTCAACG+TGG + chr1_2:51274972-51274991 Msa0075730:intron 40.0%
GGTGCTCAGAAAGTAATGAA+TGG - chr1_2:51274679-51274698 None:intergenic 40.0%
CAGGCACAATGATCATGTTA+CGG - chr1_2:51275800-51275819 None:intergenic 40.0%
ATCATTGTGCCTGAGATGAT+CGG + chr1_2:51275807-51275826 Msa0075730:CDS 40.0%
ATAATGCTACCGATCATCTC+AGG - chr1_2:51275819-51275838 None:intergenic 40.0%
GAGATGATCGGTAGCATTAT+TGG + chr1_2:51275819-51275838 Msa0075730:CDS 40.0%
! AGATAGTGGCCAATCATTTC+AGG - chr1_2:51275885-51275904 None:intergenic 40.0%
! TGAAATGATTGGCCACTATC+TGG + chr1_2:51275884-51275903 Msa0075730:CDS 40.0%
TCTTACAAGCCTGTTAAGCA+CGG + chr1_2:51275921-51275940 Msa0075730:CDS 40.0%
CTTCAAAGGAATAAACCTGG+AGG - chr1_2:51275973-51275992 None:intergenic 40.0%
AGCAAGTTTCACTGCAATCA+TGG + chr1_2:51274266-51274285 Msa0075730:exon 40.0%
! TGTTGATCTCGATGCTCTTT+TGG + chr1_2:51274456-51274475 Msa0075730:CDS 40.0%
AAGCTTGACAAGTTCATCAG+TGG - chr1_2:51274488-51274507 None:intergenic 40.0%
GGTGCTCAGAAAGTAATGAA+TGG - chr1_2:51274679-51274698 None:intergenic 40.0%
!! GTTCATTTCAGGTTTCAACG+TGG + chr1_2:51274972-51274991 Msa0075730:intron 40.0%
GGTGCTCAGAAAGTAATGAA+TGG - chr1_2:51274679-51274698 None:intergenic 40.0%
CAGGCACAATGATCATGTTA+CGG - chr1_2:51275800-51275819 None:intergenic 40.0%
ATCATTGTGCCTGAGATGAT+CGG + chr1_2:51275807-51275826 Msa0075730:CDS 40.0%
ATAATGCTACCGATCATCTC+AGG - chr1_2:51275819-51275838 None:intergenic 40.0%
GAGATGATCGGTAGCATTAT+TGG + chr1_2:51275819-51275838 Msa0075730:CDS 40.0%
! AGATAGTGGCCAATCATTTC+AGG - chr1_2:51275885-51275904 None:intergenic 40.0%
! TGAAATGATTGGCCACTATC+TGG + chr1_2:51275884-51275903 Msa0075730:CDS 40.0%
TCTTACAAGCCTGTTAAGCA+CGG + chr1_2:51275921-51275940 Msa0075730:CDS 40.0%
CTTCAAAGGAATAAACCTGG+AGG - chr1_2:51275973-51275992 None:intergenic 40.0%
AGCTTCTTGATGAGTGCCAT+AGG - chr1_2:51275011-51275030 None:intergenic 45.0%
GCAGCCGAATGTATCTGTAA+AGG - chr1_2:51275459-51275478 None:intergenic 45.0%
GCACAATGATCATGTTACGG+AGG - chr1_2:51275797-51275816 None:intergenic 45.0%
AGCGAATTCGGTAGACAAGT+TGG + chr1_2:51276260-51276279 Msa0075730:three_prime_UTR 45.0%
AGCTTCTTGATGAGTGCCAT+AGG - chr1_2:51275011-51275030 None:intergenic 45.0%
GCAGCCGAATGTATCTGTAA+AGG - chr1_2:51275459-51275478 None:intergenic 45.0%
GCACAATGATCATGTTACGG+AGG - chr1_2:51275797-51275816 None:intergenic 45.0%
AGCGAATTCGGTAGACAAGT+TGG + chr1_2:51276260-51276279 Msa0075730:three_prime_UTR 45.0%
ACCTCTCAGGGATTGTGCTA+GGG - chr1_2:51274216-51274235 None:intergenic 50.0%
TACCTCTCAGGGATTGTGCT+AGG - chr1_2:51274217-51274236 None:intergenic 50.0%
! GCAGTGAAACTTGCTCGATC+TGG - chr1_2:51274261-51274280 None:intergenic 50.0%
!! GAGCATCGAGATCAACACCA+CGG - chr1_2:51274453-51274472 None:intergenic 50.0%
TGATGAGTGCCATAGGCTTG+CGG - chr1_2:51275004-51275023 None:intergenic 50.0%
ACTCATCAAGAAGCTCCGCA+AGG + chr1_2:51275016-51275035 Msa0075730:CDS 50.0%
ACAGATAACTCACCGCCTTG+CGG - chr1_2:51275034-51275053 None:intergenic 50.0%
!! ACAGAAAAGGGAAGCACCTC+CGG + chr1_2:51275743-51275762 Msa0075730:intron 50.0%
ACAAGCCTGTTAAGCACGGT+AGG + chr1_2:51275925-51275944 Msa0075730:CDS 50.0%
GAGTGAGTAGCACCAATACC+GGG - chr1_2:51275951-51275970 None:intergenic 50.0%
GGAGTGAGTAGCACCAATAC+CGG - chr1_2:51275952-51275971 None:intergenic 50.0%
CTTGTCTACCGAATTCGCTC+AGG - chr1_2:51276259-51276278 None:intergenic 50.0%
ACCTCTCAGGGATTGTGCTA+GGG - chr1_2:51274216-51274235 None:intergenic 50.0%
TACCTCTCAGGGATTGTGCT+AGG - chr1_2:51274217-51274236 None:intergenic 50.0%
! GCAGTGAAACTTGCTCGATC+TGG - chr1_2:51274261-51274280 None:intergenic 50.0%
!! GAGCATCGAGATCAACACCA+CGG - chr1_2:51274453-51274472 None:intergenic 50.0%
TGATGAGTGCCATAGGCTTG+CGG - chr1_2:51275004-51275023 None:intergenic 50.0%
ACTCATCAAGAAGCTCCGCA+AGG + chr1_2:51275016-51275035 Msa0075730:CDS 50.0%
ACAGATAACTCACCGCCTTG+CGG - chr1_2:51275034-51275053 None:intergenic 50.0%
!! ACAGAAAAGGGAAGCACCTC+CGG + chr1_2:51275743-51275762 Msa0075730:intron 50.0%
ACAAGCCTGTTAAGCACGGT+AGG + chr1_2:51275925-51275944 Msa0075730:CDS 50.0%
GAGTGAGTAGCACCAATACC+GGG - chr1_2:51275951-51275970 None:intergenic 50.0%
GGAGTGAGTAGCACCAATAC+CGG - chr1_2:51275952-51275971 None:intergenic 50.0%
CTTGTCTACCGAATTCGCTC+AGG - chr1_2:51276259-51276278 None:intergenic 50.0%
ACCCTAGCACAATCCCTGAG+AGG + chr1_2:51274212-51274231 Msa0075730:five_prime_UTR 55.0%
CGGCTGCTTCTTACCTCTCA+GGG - chr1_2:51274228-51274247 None:intergenic 55.0%
!! CTCGATCTGGTTCTTGCTCG+CGG - chr1_2:51274248-51274267 None:intergenic 55.0%
TCAGGCGGATGTTGAACCAG+AGG + chr1_2:51274378-51274397 Msa0075730:intron 55.0%
GAACCAGAGGTAGCTGCACA+AGG + chr1_2:51274391-51274410 Msa0075730:CDS 55.0%
ACTCCTTGTGCAGCTACCTC+TGG - chr1_2:51274397-51274416 None:intergenic 55.0%
TCTCTGCTCGTGCACGTAGA+AGG + chr1_2:51274509-51274528 Msa0075730:CDS 55.0%
TGGTTTGACCCGCAAGCCTA+TGG + chr1_2:51274992-51275011 Msa0075730:CDS 55.0%
GATGAGTGCCATAGGCTTGC+GGG - chr1_2:51275003-51275022 None:intergenic 55.0%
CATCAAGAAGCTCCGCAAGG+CGG + chr1_2:51275019-51275038 Msa0075730:CDS 55.0%
!! CAGAAAAGGGAAGCACCTCC+GGG + chr1_2:51275744-51275763 Msa0075730:intron 55.0%
!! ACAGGTTCTGGCTTCTCACC+CGG - chr1_2:51275765-51275784 None:intergenic 55.0%
GTGAGAAGCCAGAACCTGTG+AGG + chr1_2:51275766-51275785 Msa0075730:CDS 55.0%
AGGTGAGTCCTCACAGGTTC+TGG - chr1_2:51275777-51275796 None:intergenic 55.0%
TTACGGAGGTGAGTCCTCAC+AGG - chr1_2:51275783-51275802 None:intergenic 55.0%
!! TTGGTGCTACTCACTCCTCC+AGG + chr1_2:51275955-51275974 Msa0075730:CDS 55.0%
ACCCTAGCACAATCCCTGAG+AGG + chr1_2:51274212-51274231 Msa0075730:five_prime_UTR 55.0%
CGGCTGCTTCTTACCTCTCA+GGG - chr1_2:51274228-51274247 None:intergenic 55.0%
!! CTCGATCTGGTTCTTGCTCG+CGG - chr1_2:51274248-51274267 None:intergenic 55.0%
TCAGGCGGATGTTGAACCAG+AGG + chr1_2:51274378-51274397 Msa0075730:intron 55.0%
GAACCAGAGGTAGCTGCACA+AGG + chr1_2:51274391-51274410 Msa0075730:CDS 55.0%
ACTCCTTGTGCAGCTACCTC+TGG - chr1_2:51274397-51274416 None:intergenic 55.0%
TCTCTGCTCGTGCACGTAGA+AGG + chr1_2:51274509-51274528 Msa0075730:CDS 55.0%
TGGTTTGACCCGCAAGCCTA+TGG + chr1_2:51274992-51275011 Msa0075730:CDS 55.0%
GATGAGTGCCATAGGCTTGC+GGG - chr1_2:51275003-51275022 None:intergenic 55.0%
CATCAAGAAGCTCCGCAAGG+CGG + chr1_2:51275019-51275038 Msa0075730:CDS 55.0%
!! CAGAAAAGGGAAGCACCTCC+GGG + chr1_2:51275744-51275763 Msa0075730:intron 55.0%
!! ACAGGTTCTGGCTTCTCACC+CGG - chr1_2:51275765-51275784 None:intergenic 55.0%
GTGAGAAGCCAGAACCTGTG+AGG + chr1_2:51275766-51275785 Msa0075730:CDS 55.0%
AGGTGAGTCCTCACAGGTTC+TGG - chr1_2:51275777-51275796 None:intergenic 55.0%
TTACGGAGGTGAGTCCTCAC+AGG - chr1_2:51275783-51275802 None:intergenic 55.0%
!! TTGGTGCTACTCACTCCTCC+AGG + chr1_2:51275955-51275974 Msa0075730:CDS 55.0%
GCGGCTGCTTCTTACCTCTC+AGG - chr1_2:51274229-51274248 None:intergenic 60.0%
CCTGTTAAGCACGGTAGGCC+CGG + chr1_2:51275930-51275949 Msa0075730:CDS 60.0%
AAGCACGGTAGGCCCGGTAT+TGG + chr1_2:51275936-51275955 Msa0075730:CDS 60.0%
GCGGCTGCTTCTTACCTCTC+AGG - chr1_2:51274229-51274248 None:intergenic 60.0%
CCTGTTAAGCACGGTAGGCC+CGG + chr1_2:51275930-51275949 Msa0075730:CDS 60.0%
AAGCACGGTAGGCCCGGTAT+TGG + chr1_2:51275936-51275955 Msa0075730:CDS 60.0%
!! GGTTCTGGCTTCTCACCCGG+AGG - chr1_2:51275762-51275781 None:intergenic 65.0%
CCGGGCCTACCGTGCTTAAC+AGG - chr1_2:51275933-51275952 None:intergenic 65.0%
!! GGTTCTGGCTTCTCACCCGG+AGG - chr1_2:51275762-51275781 None:intergenic 65.0%
CCGGGCCTACCGTGCTTAAC+AGG - chr1_2:51275933-51275952 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 51274197 51276385 51274197 ID=Msa0075730;Name=Msa0075730
chr1_2 mRNA 51274197 51276385 51274197 ID=Msa0075730-mRNA-1;Parent=Msa0075730;Name=Msa0075730-mRNA-1;_AED=0.00;_eAED=0.00;_QI=88|1|1|1|1|1|4|390|152
chr1_2 exon 51274197 51274287 51274197 ID=Msa0075730-mRNA-1:exon:15752;Parent=Msa0075730-mRNA-1
chr1_2 exon 51274382 51274530 51274382 ID=Msa0075730-mRNA-1:exon:15753;Parent=Msa0075730-mRNA-1
chr1_2 exon 51274983 51275040 51274983 ID=Msa0075730-mRNA-1:exon:15754;Parent=Msa0075730-mRNA-1
chr1_2 exon 51275747 51276385 51275747 ID=Msa0075730-mRNA-1:exon:15755;Parent=Msa0075730-mRNA-1
chr1_2 five_prime_UTR 51274197 51274284 51274197 ID=Msa0075730-mRNA-1:five_prime_utr;Parent=Msa0075730-mRNA-1
chr1_2 CDS 51274285 51274287 51274285 ID=Msa0075730-mRNA-1:cds;Parent=Msa0075730-mRNA-1
chr1_2 CDS 51274382 51274530 51274382 ID=Msa0075730-mRNA-1:cds;Parent=Msa0075730-mRNA-1
chr1_2 CDS 51274983 51275040 51274983 ID=Msa0075730-mRNA-1:cds;Parent=Msa0075730-mRNA-1
chr1_2 CDS 51275747 51275995 51275747 ID=Msa0075730-mRNA-1:cds;Parent=Msa0075730-mRNA-1
chr1_2 three_prime_UTR 51275996 51276385 51275996 ID=Msa0075730-mRNA-1:three_prime_utr;Parent=Msa0075730-mRNA-1
Gene Sequence

>Msa0075730

ATGGCGGATGTTGAACCAGAGGTAGCTGCACAAGGAGTGCCAAAGAAGAGAACATTTAAGAAGTTCAGTTTCCGTGGTGTTGATCTCGATGCTCTTTTGGATATGTCCACTGATGAACTTGTCAAGCTTTTCTCTGCTCGTGCACGTAGAAGGTTTCAACGTGGTTTGACCCGCAAGCCTATGGCACTCATCAAGAAGCTCCGCAAGGCGAAAAGGGAAGCACCTCCGGGTGAGAAGCCAGAACCTGTGAGGACTCACCTCCGTAACATGATCATTGTGCCTGAGATGATCGGTAGCATTATTGGTGTCTACAATGGAAAGACCTTCAACCAAGTTGAAATTAAGCCTGAAATGATTGGCCACTATCTGGCTGAATTTTCAATCTCTTACAAGCCTGTTAAGCACGGTAGGCCCGGTATTGGTGCTACTCACTCCTCCAGGTTTATTCCTTTGAAGTGA
Protein sequence

>Msa0075730

MADVEPEVAAQGVPKKRTFKKFSFRGVDLDALLDMSTDELVKLFSARARRRFQRGLTRKPMALIKKLRKAKREAPPGEKPEPVRTHLRNMIIVPEMIGSIIGVYNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK*