AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0123790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0123790 MtrunA17_Chr1g0184681 99.342 152 1 0 1 152 1 152 2.95e-109 307
Msa0123790 MtrunA17_Chr4g0038481 96.711 152 5 0 1 152 1 152 8.07e-105 296
Msa0123790 MtrunA17_Chr8g0391601 96.104 154 4 1 1 152 1 154 1.61e-104 295
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0123790 AT5G09510.1 94.079 152 9 0 1 152 1 152 6.82e-96 273
Msa0123790 AT1G04270.1 94.079 152 9 0 1 152 1 152 1.06e-95 273
Msa0123790 AT5G09500.1 88.816 152 15 1 1 152 1 150 9.89e-95 270
Msa0123790 AT1G04270.2 93.421 152 9 1 1 152 1 151 3.95e-93 266
Msa0123790 AT5G09490.1 82.237 152 27 0 1 152 1 152 1.17e-90 260
Msa0123790 AT5G43640.1 87.919 149 18 0 4 152 1 149 4.43e-85 246
Msa0123790 AT5G09510.2 94.915 118 6 0 35 152 1 118 4.22e-71 209
Msa0123790 AT5G63070.1 62.500 160 52 3 1 152 1 160 3.97e-63 191
Msa0123790 AT1G33850.1 80.597 67 13 0 4 70 1 67 2.53e-22 84.7

Find 71 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTAAATGTTCTCTTCTT+TGG 0.218192 1_3:-51114645 None:intergenic
TGTTGATCTCGATGCTCTTT+TGG 0.301311 1_3:+51114683 Msa0123790:CDS
TTCCCAGCATATCTTTCATT+TGG 0.318679 1_3:-51116633 None:intergenic
AGGTGAGTCCTCACAGGTTC+TGG 0.319193 1_3:-51115987 None:intergenic
ATATCAGTGAACTAAAGTTT+TGG 0.345093 1_3:+51116416 Msa0123790:three_prime_UTR
GAGATGATCGGTAGCATTAT+TGG 0.345545 1_3:+51116032 Msa0123790:CDS
CCGGGCCTACCGTGCTTAAC+AGG 0.350740 1_3:-51116143 None:intergenic
AGATAGTGGCCAATCATTTC+AGG 0.354585 1_3:-51116095 None:intergenic
GATGAGTGCCATAGGCTTGC+GGG 0.362183 1_3:-51115214 None:intergenic
TGAAATGATTGGCCACTATC+TGG 0.368994 1_3:+51116097 Msa0123790:CDS
TCTCTGCTCGTGCACGTAGA+AGG 0.369916 1_3:+51114736 Msa0123790:CDS
ACTTAAATCCTGAGCGAATT+CGG 0.375078 1_3:+51116561 Msa0123790:three_prime_UTR
TATTTGGTTTATAGATAGAT+TGG 0.376185 1_3:+51116491 Msa0123790:three_prime_UTR
GAAATTAAGCCTGAAATGAT+TGG 0.379557 1_3:+51116086 Msa0123790:CDS
CTTAATTTCAACTTGGTTGA+AGG 0.392478 1_3:-51116072 None:intergenic
TACCTCTCAGGGATTGTGCT+AGG 0.395694 1_3:-51114441 None:intergenic
GCGGCTGCTTCTTACCTCTC+AGG 0.399037 1_3:-51114453 None:intergenic
ACAGAAAAGGGAAGCACCTC+CGG 0.413302 1_3:+51115956 Msa0123790:intron
ACTCCTTGTGCAGCTACCTC+TGG 0.435684 1_3:-51114621 None:intergenic
GCAGTGAAACTTGCTCGATC+TGG 0.436867 1_3:-51114485 None:intergenic
AGTCCAAATGAAAGATATGC+TGG 0.443478 1_3:+51116630 Msa0123790:three_prime_UTR
CGGCTGCTTCTTACCTCTCA+GGG 0.459104 1_3:-51114452 None:intergenic
ACCTCTCAGGGATTGTGCTA+GGG 0.474600 1_3:-51114440 None:intergenic
TATTATTGACCATGAAAGCT+TGG 0.476757 1_3:+51116515 Msa0123790:three_prime_UTR
CTTGTCTACCGAATTCGCTC+AGG 0.483917 1_3:-51116569 None:intergenic
TTGAAAATTCAGCCAGATAG+TGG 0.486704 1_3:-51116109 None:intergenic
TTGGTGCTACTCACTCCTCC+AGG 0.496949 1_3:+51116168 Msa0123790:CDS
TGGTTTGACCCGCAAGCCTA+TGG 0.497772 1_3:+51115206 Msa0123790:CDS
GGAGTGAGTAGCACCAATAC+CGG 0.503041 1_3:-51116162 None:intergenic
AGCGAATTCGGTAGACAAGT+TGG 0.504139 1_3:+51116573 Msa0123790:three_prime_UTR
AAGCACGGTAGGCCCGGTAT+TGG 0.508994 1_3:+51116149 Msa0123790:CDS
AATGACAATCTTCACTTCAA+AGG 0.512033 1_3:-51116197 None:intergenic
CCTGTTAAGCACGGTAGGCC+CGG 0.514282 1_3:+51116143 Msa0123790:CDS
CAGAAAAGGGAAGCACCTCC+GGG 0.515716 1_3:+51115957 Msa0123790:intron
ACAGGTTCTGGCTTCTCACC+CGG 0.516641 1_3:-51115975 None:intergenic
TGATGAGTGCCATAGGCTTG+CGG 0.517926 1_3:-51115215 None:intergenic
AGCAAGTTTCACTGCAATCA+TGG 0.522868 1_3:+51114493 Msa0123790:exon
GTTTGCATTGAAAGATAATG+TGG 0.529819 1_3:-51116226 None:intergenic
ATAATGCTACCGATCATCTC+AGG 0.534264 1_3:-51116029 None:intergenic
CAGGCACAATGATCATGTTA+CGG 0.535956 1_3:-51116010 None:intergenic
TTTCAGGCTTAATTTCAACT+TGG 0.556240 1_3:-51116079 None:intergenic
TTTGCATTGAAAGATAATGT+GGG 0.577878 1_3:-51116225 None:intergenic
AGCATTATTGGTGTCTACAA+TGG 0.579267 1_3:+51116044 Msa0123790:CDS
TTTAAGAAGTTCAGTTTCCG+TGG 0.586451 1_3:+51114660 Msa0123790:CDS
TCACTTCAAAGGAATAAACC+TGG 0.587310 1_3:-51116186 None:intergenic
TTACGGAGGTGAGTCCTCAC+AGG 0.595096 1_3:-51115993 None:intergenic
AGCTTCTTGATGAGTGCCAT+AGG 0.595409 1_3:-51115222 None:intergenic
ATAAACCAAATAAAATCAAG+AGG 0.595454 1_3:-51116480 None:intergenic
GTCCAAATGAAAGATATGCT+GGG 0.595723 1_3:+51116631 Msa0123790:three_prime_UTR
TGTATATATTCATGAACTAG+AGG 0.599285 1_3:+51116598 Msa0123790:three_prime_UTR
GAACCAGAGGTAGCTGCACA+AGG 0.605663 1_3:+51114618 Msa0123790:CDS
CTCGATCTGGTTCTTGCTCG+CGG 0.612106 1_3:-51114472 None:intergenic
GAGTGAGTAGCACCAATACC+GGG 0.613332 1_3:-51116161 None:intergenic
TGATATTGTTATGACACTAG+AGG 0.627040 1_3:+51116393 Msa0123790:three_prime_UTR
AAGCTTGACAAGTTCATCAG+TGG 0.629931 1_3:-51114712 None:intergenic
ACAAGCCTGTTAAGCACGGT+AGG 0.632440 1_3:+51116138 Msa0123790:CDS
TTGCATTGAAAGATAATGTG+GGG 0.633934 1_3:-51116224 None:intergenic
TCTTACAAGCCTGTTAAGCA+CGG 0.639372 1_3:+51116134 Msa0123790:CDS
TCAGGCGGATGTTGAACCAG+AGG 0.645787 1_3:+51114605 Msa0123790:intron
ACTCATCAAGAAGCTCCGCA+AGG 0.648877 1_3:+51115230 Msa0123790:CDS
ATCATTGTGCCTGAGATGAT+CGG 0.650070 1_3:+51116020 Msa0123790:CDS
GGTTCTGGCTTCTCACCCGG+AGG 0.654327 1_3:-51115972 None:intergenic
ACAGATAACTCACCGCCTTG+CGG 0.662337 1_3:-51115245 None:intergenic
TCCTTCACAATCCACAACAG+AGG 0.683089 1_3:+51114409 None:intergenic
GTGAGAAGCCAGAACCTGTG+AGG 0.700359 1_3:+51115979 Msa0123790:CDS
ACCCTAGCACAATCCCTGAG+AGG 0.707194 1_3:+51114439 Msa0123790:five_prime_UTR
GCACAATGATCATGTTACGG+AGG 0.711118 1_3:-51116007 None:intergenic
CTTCAAAGGAATAAACCTGG+AGG 0.714921 1_3:-51116183 None:intergenic
CATCAAGAAGCTCCGCAAGG+CGG 0.735053 1_3:+51115233 Msa0123790:CDS
GAGCATCGAGATCAACACCA+CGG 0.739088 1_3:-51114677 None:intergenic
GTTCATTTCAGGTTTCAACG+TGG 0.745899 1_3:+51115186 Msa0123790:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCATTTTGTTTTATTTTA+TGG + chr1_3:51114968-51114987 Msa0123790:intron 10.0%
!!! TTCATTTTGTTTTATTTTAT+GGG + chr1_3:51114969-51114988 Msa0123790:intron 10.0%
!!! TTTCATTTTGTTTTATTTTA+TGG + chr1_3:51114968-51114987 Msa0123790:intron 10.0%
!!! TTCATTTTGTTTTATTTTAT+GGG + chr1_3:51114969-51114988 Msa0123790:intron 10.0%
!! TAACCTAAAAATGATGAAAA+TGG - chr1_3:51114915-51114934 None:intergenic 20.0%
!!! TTCATCATTTTTAGGTTATT+TGG + chr1_3:51114917-51114936 Msa0123790:intron 20.0%
!!! TATCATTTTTGTTTTGTTGT+TGG + chr1_3:51115073-51115092 Msa0123790:intron 20.0%
!! GTAAATGTAAAAATGAGAAA+TGG - chr1_3:51115154-51115173 None:intergenic 20.0%
!! TTTAACAATATGTTCATTTC+AGG + chr1_3:51115175-51115194 Msa0123790:intron 20.0%
!! TTTGGAAATTGTTTATTTAG+CGG + chr1_3:51115382-51115401 Msa0123790:intron 20.0%
!! ACAATATACTGTCTTAAATT+TGG + chr1_3:51115605-51115624 Msa0123790:intron 20.0%
!! CAATATACTGTCTTAAATTT+GGG + chr1_3:51115606-51115625 Msa0123790:intron 20.0%
!! AATATACTGTCTTAAATTTG+GGG + chr1_3:51115607-51115626 Msa0123790:intron 20.0%
!!! TTTAAAATGATAGCATGATT+GGG - chr1_3:51115859-51115878 None:intergenic 20.0%
!!! ATTTAAAATGATAGCATGAT+TGG - chr1_3:51115860-51115879 None:intergenic 20.0%
!! TTGTATATTGTTACAGAAAA+GGG + chr1_3:51115944-51115963 Msa0123790:intron 20.0%
!! ATAAACCAAATAAAATCAAG+AGG - chr1_3:51116483-51116502 None:intergenic 20.0%
!!! TATTTGGTTTATAGATAGAT+TGG + chr1_3:51116491-51116510 Msa0123790:three_prime_UTR 20.0%
!! TAACCTAAAAATGATGAAAA+TGG - chr1_3:51114915-51114934 None:intergenic 20.0%
!!! TTCATCATTTTTAGGTTATT+TGG + chr1_3:51114917-51114936 Msa0123790:intron 20.0%
!!! TATCATTTTTGTTTTGTTGT+TGG + chr1_3:51115073-51115092 Msa0123790:intron 20.0%
!! GTAAATGTAAAAATGAGAAA+TGG - chr1_3:51115154-51115173 None:intergenic 20.0%
!! TTTAACAATATGTTCATTTC+AGG + chr1_3:51115175-51115194 Msa0123790:intron 20.0%
!! TTTGGAAATTGTTTATTTAG+CGG + chr1_3:51115382-51115401 Msa0123790:intron 20.0%
!! ACAATATACTGTCTTAAATT+TGG + chr1_3:51115605-51115624 Msa0123790:intron 20.0%
!! CAATATACTGTCTTAAATTT+GGG + chr1_3:51115606-51115625 Msa0123790:intron 20.0%
!! AATATACTGTCTTAAATTTG+GGG + chr1_3:51115607-51115626 Msa0123790:intron 20.0%
!!! TTTAAAATGATAGCATGATT+GGG - chr1_3:51115859-51115878 None:intergenic 20.0%
!!! ATTTAAAATGATAGCATGAT+TGG - chr1_3:51115860-51115879 None:intergenic 20.0%
!! TTGTATATTGTTACAGAAAA+GGG + chr1_3:51115944-51115963 Msa0123790:intron 20.0%
!! ATAAACCAAATAAAATCAAG+AGG - chr1_3:51116483-51116502 None:intergenic 20.0%
!!! TATTTGGTTTATAGATAGAT+TGG + chr1_3:51116491-51116510 Msa0123790:three_prime_UTR 20.0%
!!! ATGAATTTGGTTGATTTTTC+AGG + chr1_3:51114587-51114606 Msa0123790:intron 25.0%
! TTCTTAAATGTTCTCTTCTT+TGG - chr1_3:51114648-51114667 None:intergenic 25.0%
! GAATATATGAATGATGAAGA+TGG + chr1_3:51114805-51114824 Msa0123790:intron 25.0%
! CGTTAATCTGATTTATTATG+AGG + chr1_3:51114855-51114874 Msa0123790:intron 25.0%
!! TGGCTAAATGTTGAATTTTT+AGG + chr1_3:51114937-51114956 Msa0123790:intron 25.0%
!!! TTATGTTTTGAAGAAATGTG+AGG + chr1_3:51115022-51115041 Msa0123790:intron 25.0%
!! GTTTAAGTTCTGTTACTTAT+AGG + chr1_3:51115095-51115114 Msa0123790:intron 25.0%
!! ATGTAATTGATTCATTTCAG+TGG + chr1_3:51115303-51115322 Msa0123790:intron 25.0%
!! ATTCTAGTTGTTGAGTAATT+TGG + chr1_3:51115364-51115383 Msa0123790:intron 25.0%
! CGTTAATCTGATTTATTATG+AGG + chr1_3:51114855-51114874 Msa0123790:intron 25.0%
! AAAGTAATGAATGGATATGA+AGG - chr1_3:51114885-51114904 None:intergenic 25.0%
!!! ATGTTTTAGATCAAGTAGAA+GGG - chr1_3:51115565-51115584 None:intergenic 25.0%
! ATATACTGTCTTAAATTTGG+GGG + chr1_3:51115608-51115627 Msa0123790:intron 25.0%
! TGTATGATTATGTATCTAAC+TGG + chr1_3:51115756-51115775 Msa0123790:intron 25.0%
!!! ATTTTGGGTACATAATCATA+CGG - chr1_3:51115895-51115914 None:intergenic 25.0%
!! AACACCTAACAACAAATTTT+GGG - chr1_3:51115910-51115929 None:intergenic 25.0%
! GTTGTATATTGTTACAGAAA+AGG + chr1_3:51115943-51115962 Msa0123790:intron 25.0%
! TTTGCATTGAAAGATAATGT+GGG - chr1_3:51116228-51116247 None:intergenic 25.0%
!! ATATCAGTGAACTAAAGTTT+TGG + chr1_3:51116416-51116435 Msa0123790:three_prime_UTR 25.0%
!! TATCAGTGAACTAAAGTTTT+GGG + chr1_3:51116417-51116436 Msa0123790:three_prime_UTR 25.0%
!! TGTTTCCTCTTGATTTTATT+TGG + chr1_3:51116475-51116494 Msa0123790:three_prime_UTR 25.0%
!!! TAATTTTTTCCAAGCTTTCA+TGG - chr1_3:51116527-51116546 None:intergenic 25.0%
! AAGCTTGGAAAAAATTATTG+TGG + chr1_3:51116530-51116549 Msa0123790:three_prime_UTR 25.0%
! AGCTTGGAAAAAATTATTGT+GGG + chr1_3:51116531-51116550 Msa0123790:three_prime_UTR 25.0%
! TGTATATATTCATGAACTAG+AGG + chr1_3:51116598-51116617 Msa0123790:three_prime_UTR 25.0%
!!! ATGAATTTGGTTGATTTTTC+AGG + chr1_3:51114587-51114606 Msa0123790:intron 25.0%
! TTCTTAAATGTTCTCTTCTT+TGG - chr1_3:51114648-51114667 None:intergenic 25.0%
! GAATATATGAATGATGAAGA+TGG + chr1_3:51114805-51114824 Msa0123790:intron 25.0%
! CGTTAATCTGATTTATTATG+AGG + chr1_3:51114855-51114874 Msa0123790:intron 25.0%
!! TGGCTAAATGTTGAATTTTT+AGG + chr1_3:51114937-51114956 Msa0123790:intron 25.0%
!!! TTATGTTTTGAAGAAATGTG+AGG + chr1_3:51115022-51115041 Msa0123790:intron 25.0%
!! GTTTAAGTTCTGTTACTTAT+AGG + chr1_3:51115095-51115114 Msa0123790:intron 25.0%
!! ATGTAATTGATTCATTTCAG+TGG + chr1_3:51115303-51115322 Msa0123790:intron 25.0%
!! ATTCTAGTTGTTGAGTAATT+TGG + chr1_3:51115364-51115383 Msa0123790:intron 25.0%
! CGTTAATCTGATTTATTATG+AGG + chr1_3:51114855-51114874 Msa0123790:intron 25.0%
! AAAGTAATGAATGGATATGA+AGG - chr1_3:51114885-51114904 None:intergenic 25.0%
!!! ATGTTTTAGATCAAGTAGAA+GGG - chr1_3:51115565-51115584 None:intergenic 25.0%
! ATATACTGTCTTAAATTTGG+GGG + chr1_3:51115608-51115627 Msa0123790:intron 25.0%
! TGTATGATTATGTATCTAAC+TGG + chr1_3:51115756-51115775 Msa0123790:intron 25.0%
!!! ATTTTGGGTACATAATCATA+CGG - chr1_3:51115895-51115914 None:intergenic 25.0%
!! AACACCTAACAACAAATTTT+GGG - chr1_3:51115910-51115929 None:intergenic 25.0%
! GTTGTATATTGTTACAGAAA+AGG + chr1_3:51115943-51115962 Msa0123790:intron 25.0%
! TTTGCATTGAAAGATAATGT+GGG - chr1_3:51116228-51116247 None:intergenic 25.0%
!! ATATCAGTGAACTAAAGTTT+TGG + chr1_3:51116416-51116435 Msa0123790:three_prime_UTR 25.0%
!! TATCAGTGAACTAAAGTTTT+GGG + chr1_3:51116417-51116436 Msa0123790:three_prime_UTR 25.0%
!! TGTTTCCTCTTGATTTTATT+TGG + chr1_3:51116475-51116494 Msa0123790:three_prime_UTR 25.0%
!!! TAATTTTTTCCAAGCTTTCA+TGG - chr1_3:51116527-51116546 None:intergenic 25.0%
! AAGCTTGGAAAAAATTATTG+TGG + chr1_3:51116530-51116549 Msa0123790:three_prime_UTR 25.0%
! AGCTTGGAAAAAATTATTGT+GGG + chr1_3:51116531-51116550 Msa0123790:three_prime_UTR 25.0%
! TGTATATATTCATGAACTAG+AGG + chr1_3:51116598-51116617 Msa0123790:three_prime_UTR 25.0%
AAGAGAAAGAGATAGAGTTA+GGG - chr1_3:51114525-51114544 None:intergenic 30.0%
!!! AATTTGGTTGATTTTTCAGG+CGG + chr1_3:51114590-51114609 Msa0123790:intron 30.0%
!! GCACCATTTTCATCATTTTT+AGG + chr1_3:51114909-51114928 Msa0123790:intron 30.0%
TATGGGATTACTAGAAATGT+TGG + chr1_3:51114986-51115005 Msa0123790:intron 30.0%
! TGTTACTTATAGGATCCTTA+AGG + chr1_3:51115105-51115124 Msa0123790:intron 30.0%
GAAATTGTTTATTTAGCGGT+TGG + chr1_3:51115386-51115405 Msa0123790:intron 30.0%
! TCTTTTGCGTTTGTATCTTT+TGG + chr1_3:51115454-51115473 Msa0123790:intron 30.0%
! CTTTTGCGTTTGTATCTTTT+GGG + chr1_3:51115455-51115474 Msa0123790:intron 30.0%
!! GATGTTTTAGATCAAGTAGA+AGG - chr1_3:51115566-51115585 None:intergenic 30.0%
GCATTTGAACTTAACAGATA+TGG + chr1_3:51115634-51115653 Msa0123790:intron 30.0%
ATCTCCTTTACAGATACATT+CGG + chr1_3:51115665-51115684 Msa0123790:intron 30.0%
!! TGTACCCAAAATTTGTTGTT+AGG + chr1_3:51115903-51115922 Msa0123790:intron 30.0%
! GAACACCTAACAACAAATTT+TGG - chr1_3:51115911-51115930 None:intergenic 30.0%
CTTAATTTCAACTTGGTTGA+AGG - chr1_3:51116075-51116094 None:intergenic 30.0%
TTTCAGGCTTAATTTCAACT+TGG - chr1_3:51116082-51116101 None:intergenic 30.0%
GAAATTAAGCCTGAAATGAT+TGG + chr1_3:51116086-51116105 Msa0123790:CDS 30.0%
AATGACAATCTTCACTTCAA+AGG - chr1_3:51116200-51116219 None:intergenic 30.0%
TTGCATTGAAAGATAATGTG+GGG - chr1_3:51116227-51116246 None:intergenic 30.0%
GTTTGCATTGAAAGATAATG+TGG - chr1_3:51116229-51116248 None:intergenic 30.0%
!!! CAAACTTTTGCTTGTTTTAC+TGG + chr1_3:51116244-51116263 Msa0123790:three_prime_UTR 30.0%
TGATATTGTTATGACACTAG+AGG + chr1_3:51116393-51116412 Msa0123790:three_prime_UTR 30.0%
! TTGTCTATTGATGAGCTTTT+TGG + chr1_3:51116446-51116465 Msa0123790:three_prime_UTR 30.0%
TATTATTGACCATGAAAGCT+TGG + chr1_3:51116515-51116534 Msa0123790:three_prime_UTR 30.0%
AAGAGAAAGAGATAGAGTTA+GGG - chr1_3:51114525-51114544 None:intergenic 30.0%
!!! AATTTGGTTGATTTTTCAGG+CGG + chr1_3:51114590-51114609 Msa0123790:intron 30.0%
!! GCACCATTTTCATCATTTTT+AGG + chr1_3:51114909-51114928 Msa0123790:intron 30.0%
TATGGGATTACTAGAAATGT+TGG + chr1_3:51114986-51115005 Msa0123790:intron 30.0%
! TGTTACTTATAGGATCCTTA+AGG + chr1_3:51115105-51115124 Msa0123790:intron 30.0%
GAAATTGTTTATTTAGCGGT+TGG + chr1_3:51115386-51115405 Msa0123790:intron 30.0%
! TCTTTTGCGTTTGTATCTTT+TGG + chr1_3:51115454-51115473 Msa0123790:intron 30.0%
! CTTTTGCGTTTGTATCTTTT+GGG + chr1_3:51115455-51115474 Msa0123790:intron 30.0%
!! GATGTTTTAGATCAAGTAGA+AGG - chr1_3:51115566-51115585 None:intergenic 30.0%
GCATTTGAACTTAACAGATA+TGG + chr1_3:51115634-51115653 Msa0123790:intron 30.0%
ATCTCCTTTACAGATACATT+CGG + chr1_3:51115665-51115684 Msa0123790:intron 30.0%
!! TGTACCCAAAATTTGTTGTT+AGG + chr1_3:51115903-51115922 Msa0123790:intron 30.0%
! GAACACCTAACAACAAATTT+TGG - chr1_3:51115911-51115930 None:intergenic 30.0%
CTTAATTTCAACTTGGTTGA+AGG - chr1_3:51116075-51116094 None:intergenic 30.0%
TTTCAGGCTTAATTTCAACT+TGG - chr1_3:51116082-51116101 None:intergenic 30.0%
GAAATTAAGCCTGAAATGAT+TGG + chr1_3:51116086-51116105 Msa0123790:CDS 30.0%
AATGACAATCTTCACTTCAA+AGG - chr1_3:51116200-51116219 None:intergenic 30.0%
TTGCATTGAAAGATAATGTG+GGG - chr1_3:51116227-51116246 None:intergenic 30.0%
GTTTGCATTGAAAGATAATG+TGG - chr1_3:51116229-51116248 None:intergenic 30.0%
!!! CAAACTTTTGCTTGTTTTAC+TGG + chr1_3:51116244-51116263 Msa0123790:three_prime_UTR 30.0%
TGATATTGTTATGACACTAG+AGG + chr1_3:51116393-51116412 Msa0123790:three_prime_UTR 30.0%
! TTGTCTATTGATGAGCTTTT+TGG + chr1_3:51116446-51116465 Msa0123790:three_prime_UTR 30.0%
TATTATTGACCATGAAAGCT+TGG + chr1_3:51116515-51116534 Msa0123790:three_prime_UTR 30.0%
GAAGAGAAAGAGATAGAGTT+AGG - chr1_3:51114526-51114545 None:intergenic 35.0%
ATTCAGCAATGCGAAATTCA+CGG - chr1_3:51114566-51114585 None:intergenic 35.0%
GCATTGCTGAATGATGAATT+TGG + chr1_3:51114574-51114593 Msa0123790:intron 35.0%
TTTAAGAAGTTCAGTTTCCG+TGG + chr1_3:51114660-51114679 Msa0123790:CDS 35.0%
AGAATTAACACGAGTCCTTA+AGG - chr1_3:51115123-51115142 None:intergenic 35.0%
TGTATCTAACTGGTTGTGTT+AGG + chr1_3:51115766-51115785 Msa0123790:intron 35.0%
! AGCATTATTGGTGTCTACAA+TGG + chr1_3:51116044-51116063 Msa0123790:CDS 35.0%
TTGAAAATTCAGCCAGATAG+TGG - chr1_3:51116112-51116131 None:intergenic 35.0%
TCACTTCAAAGGAATAAACC+TGG - chr1_3:51116189-51116208 None:intergenic 35.0%
ACTTAAATCCTGAGCGAATT+CGG + chr1_3:51116561-51116580 Msa0123790:three_prime_UTR 35.0%
AGTCCAAATGAAAGATATGC+TGG + chr1_3:51116630-51116649 Msa0123790:three_prime_UTR 35.0%
GTCCAAATGAAAGATATGCT+GGG + chr1_3:51116631-51116650 Msa0123790:three_prime_UTR 35.0%
TTCCCAGCATATCTTTCATT+TGG - chr1_3:51116636-51116655 None:intergenic 35.0%
GAAGAGAAAGAGATAGAGTT+AGG - chr1_3:51114526-51114545 None:intergenic 35.0%
ATTCAGCAATGCGAAATTCA+CGG - chr1_3:51114566-51114585 None:intergenic 35.0%
GCATTGCTGAATGATGAATT+TGG + chr1_3:51114574-51114593 Msa0123790:intron 35.0%
TTTAAGAAGTTCAGTTTCCG+TGG + chr1_3:51114660-51114679 Msa0123790:CDS 35.0%
AGAATTAACACGAGTCCTTA+AGG - chr1_3:51115123-51115142 None:intergenic 35.0%
TGTATCTAACTGGTTGTGTT+AGG + chr1_3:51115766-51115785 Msa0123790:intron 35.0%
! AGCATTATTGGTGTCTACAA+TGG + chr1_3:51116044-51116063 Msa0123790:CDS 35.0%
TTGAAAATTCAGCCAGATAG+TGG - chr1_3:51116112-51116131 None:intergenic 35.0%
TCACTTCAAAGGAATAAACC+TGG - chr1_3:51116189-51116208 None:intergenic 35.0%
ACTTAAATCCTGAGCGAATT+CGG + chr1_3:51116561-51116580 Msa0123790:three_prime_UTR 35.0%
AGTCCAAATGAAAGATATGC+TGG + chr1_3:51116630-51116649 Msa0123790:three_prime_UTR 35.0%
GTCCAAATGAAAGATATGCT+GGG + chr1_3:51116631-51116650 Msa0123790:three_prime_UTR 35.0%
TTCCCAGCATATCTTTCATT+TGG - chr1_3:51116636-51116655 None:intergenic 35.0%
AGCAAGTTTCACTGCAATCA+TGG + chr1_3:51114493-51114512 Msa0123790:exon 40.0%
! TGTTGATCTCGATGCTCTTT+TGG + chr1_3:51114683-51114702 Msa0123790:CDS 40.0%
AAGCTTGACAAGTTCATCAG+TGG - chr1_3:51114715-51114734 None:intergenic 40.0%
GGTGCTCAGAAAGTAATGAA+TGG - chr1_3:51114894-51114913 None:intergenic 40.0%
!! GTTCATTTCAGGTTTCAACG+TGG + chr1_3:51115186-51115205 Msa0123790:intron 40.0%
GGTGCTCAGAAAGTAATGAA+TGG - chr1_3:51114894-51114913 None:intergenic 40.0%
CAGGCACAATGATCATGTTA+CGG - chr1_3:51116013-51116032 None:intergenic 40.0%
ATCATTGTGCCTGAGATGAT+CGG + chr1_3:51116020-51116039 Msa0123790:CDS 40.0%
ATAATGCTACCGATCATCTC+AGG - chr1_3:51116032-51116051 None:intergenic 40.0%
GAGATGATCGGTAGCATTAT+TGG + chr1_3:51116032-51116051 Msa0123790:CDS 40.0%
! AGATAGTGGCCAATCATTTC+AGG - chr1_3:51116098-51116117 None:intergenic 40.0%
! TGAAATGATTGGCCACTATC+TGG + chr1_3:51116097-51116116 Msa0123790:CDS 40.0%
TCTTACAAGCCTGTTAAGCA+CGG + chr1_3:51116134-51116153 Msa0123790:CDS 40.0%
CTTCAAAGGAATAAACCTGG+AGG - chr1_3:51116186-51116205 None:intergenic 40.0%
AGCAAGTTTCACTGCAATCA+TGG + chr1_3:51114493-51114512 Msa0123790:exon 40.0%
! TGTTGATCTCGATGCTCTTT+TGG + chr1_3:51114683-51114702 Msa0123790:CDS 40.0%
AAGCTTGACAAGTTCATCAG+TGG - chr1_3:51114715-51114734 None:intergenic 40.0%
GGTGCTCAGAAAGTAATGAA+TGG - chr1_3:51114894-51114913 None:intergenic 40.0%
!! GTTCATTTCAGGTTTCAACG+TGG + chr1_3:51115186-51115205 Msa0123790:intron 40.0%
GGTGCTCAGAAAGTAATGAA+TGG - chr1_3:51114894-51114913 None:intergenic 40.0%
CAGGCACAATGATCATGTTA+CGG - chr1_3:51116013-51116032 None:intergenic 40.0%
ATCATTGTGCCTGAGATGAT+CGG + chr1_3:51116020-51116039 Msa0123790:CDS 40.0%
ATAATGCTACCGATCATCTC+AGG - chr1_3:51116032-51116051 None:intergenic 40.0%
GAGATGATCGGTAGCATTAT+TGG + chr1_3:51116032-51116051 Msa0123790:CDS 40.0%
! AGATAGTGGCCAATCATTTC+AGG - chr1_3:51116098-51116117 None:intergenic 40.0%
! TGAAATGATTGGCCACTATC+TGG + chr1_3:51116097-51116116 Msa0123790:CDS 40.0%
TCTTACAAGCCTGTTAAGCA+CGG + chr1_3:51116134-51116153 Msa0123790:CDS 40.0%
CTTCAAAGGAATAAACCTGG+AGG - chr1_3:51116186-51116205 None:intergenic 40.0%
AGCTTCTTGATGAGTGCCAT+AGG - chr1_3:51115225-51115244 None:intergenic 45.0%
GCAGCCGAATGTATCTGTAA+AGG - chr1_3:51115672-51115691 None:intergenic 45.0%
GCACAATGATCATGTTACGG+AGG - chr1_3:51116010-51116029 None:intergenic 45.0%
AGCGAATTCGGTAGACAAGT+TGG + chr1_3:51116573-51116592 Msa0123790:three_prime_UTR 45.0%
AGCTTCTTGATGAGTGCCAT+AGG - chr1_3:51115225-51115244 None:intergenic 45.0%
GCAGCCGAATGTATCTGTAA+AGG - chr1_3:51115672-51115691 None:intergenic 45.0%
GCACAATGATCATGTTACGG+AGG - chr1_3:51116010-51116029 None:intergenic 45.0%
AGCGAATTCGGTAGACAAGT+TGG + chr1_3:51116573-51116592 Msa0123790:three_prime_UTR 45.0%
ACCTCTCAGGGATTGTGCTA+GGG - chr1_3:51114443-51114462 None:intergenic 50.0%
TACCTCTCAGGGATTGTGCT+AGG - chr1_3:51114444-51114463 None:intergenic 50.0%
! GCAGTGAAACTTGCTCGATC+TGG - chr1_3:51114488-51114507 None:intergenic 50.0%
!! GAGCATCGAGATCAACACCA+CGG - chr1_3:51114680-51114699 None:intergenic 50.0%
TGATGAGTGCCATAGGCTTG+CGG - chr1_3:51115218-51115237 None:intergenic 50.0%
ACTCATCAAGAAGCTCCGCA+AGG + chr1_3:51115230-51115249 Msa0123790:CDS 50.0%
ACAGATAACTCACCGCCTTG+CGG - chr1_3:51115248-51115267 None:intergenic 50.0%
!! ACAGAAAAGGGAAGCACCTC+CGG + chr1_3:51115956-51115975 Msa0123790:intron 50.0%
ACAAGCCTGTTAAGCACGGT+AGG + chr1_3:51116138-51116157 Msa0123790:CDS 50.0%
GAGTGAGTAGCACCAATACC+GGG - chr1_3:51116164-51116183 None:intergenic 50.0%
GGAGTGAGTAGCACCAATAC+CGG - chr1_3:51116165-51116184 None:intergenic 50.0%
CTTGTCTACCGAATTCGCTC+AGG - chr1_3:51116572-51116591 None:intergenic 50.0%
ACCTCTCAGGGATTGTGCTA+GGG - chr1_3:51114443-51114462 None:intergenic 50.0%
TACCTCTCAGGGATTGTGCT+AGG - chr1_3:51114444-51114463 None:intergenic 50.0%
! GCAGTGAAACTTGCTCGATC+TGG - chr1_3:51114488-51114507 None:intergenic 50.0%
!! GAGCATCGAGATCAACACCA+CGG - chr1_3:51114680-51114699 None:intergenic 50.0%
TGATGAGTGCCATAGGCTTG+CGG - chr1_3:51115218-51115237 None:intergenic 50.0%
ACTCATCAAGAAGCTCCGCA+AGG + chr1_3:51115230-51115249 Msa0123790:CDS 50.0%
ACAGATAACTCACCGCCTTG+CGG - chr1_3:51115248-51115267 None:intergenic 50.0%
!! ACAGAAAAGGGAAGCACCTC+CGG + chr1_3:51115956-51115975 Msa0123790:intron 50.0%
ACAAGCCTGTTAAGCACGGT+AGG + chr1_3:51116138-51116157 Msa0123790:CDS 50.0%
GAGTGAGTAGCACCAATACC+GGG - chr1_3:51116164-51116183 None:intergenic 50.0%
GGAGTGAGTAGCACCAATAC+CGG - chr1_3:51116165-51116184 None:intergenic 50.0%
CTTGTCTACCGAATTCGCTC+AGG - chr1_3:51116572-51116591 None:intergenic 50.0%
ACCCTAGCACAATCCCTGAG+AGG + chr1_3:51114439-51114458 Msa0123790:five_prime_UTR 55.0%
CGGCTGCTTCTTACCTCTCA+GGG - chr1_3:51114455-51114474 None:intergenic 55.0%
!! CTCGATCTGGTTCTTGCTCG+CGG - chr1_3:51114475-51114494 None:intergenic 55.0%
TCAGGCGGATGTTGAACCAG+AGG + chr1_3:51114605-51114624 Msa0123790:intron 55.0%
GAACCAGAGGTAGCTGCACA+AGG + chr1_3:51114618-51114637 Msa0123790:CDS 55.0%
ACTCCTTGTGCAGCTACCTC+TGG - chr1_3:51114624-51114643 None:intergenic 55.0%
TCTCTGCTCGTGCACGTAGA+AGG + chr1_3:51114736-51114755 Msa0123790:CDS 55.0%
TGGTTTGACCCGCAAGCCTA+TGG + chr1_3:51115206-51115225 Msa0123790:CDS 55.0%
GATGAGTGCCATAGGCTTGC+GGG - chr1_3:51115217-51115236 None:intergenic 55.0%
CATCAAGAAGCTCCGCAAGG+CGG + chr1_3:51115233-51115252 Msa0123790:CDS 55.0%
!! CAGAAAAGGGAAGCACCTCC+GGG + chr1_3:51115957-51115976 Msa0123790:intron 55.0%
!! ACAGGTTCTGGCTTCTCACC+CGG - chr1_3:51115978-51115997 None:intergenic 55.0%
GTGAGAAGCCAGAACCTGTG+AGG + chr1_3:51115979-51115998 Msa0123790:CDS 55.0%
AGGTGAGTCCTCACAGGTTC+TGG - chr1_3:51115990-51116009 None:intergenic 55.0%
TTACGGAGGTGAGTCCTCAC+AGG - chr1_3:51115996-51116015 None:intergenic 55.0%
!! TTGGTGCTACTCACTCCTCC+AGG + chr1_3:51116168-51116187 Msa0123790:CDS 55.0%
ACCCTAGCACAATCCCTGAG+AGG + chr1_3:51114439-51114458 Msa0123790:five_prime_UTR 55.0%
CGGCTGCTTCTTACCTCTCA+GGG - chr1_3:51114455-51114474 None:intergenic 55.0%
!! CTCGATCTGGTTCTTGCTCG+CGG - chr1_3:51114475-51114494 None:intergenic 55.0%
TCAGGCGGATGTTGAACCAG+AGG + chr1_3:51114605-51114624 Msa0123790:intron 55.0%
GAACCAGAGGTAGCTGCACA+AGG + chr1_3:51114618-51114637 Msa0123790:CDS 55.0%
ACTCCTTGTGCAGCTACCTC+TGG - chr1_3:51114624-51114643 None:intergenic 55.0%
TCTCTGCTCGTGCACGTAGA+AGG + chr1_3:51114736-51114755 Msa0123790:CDS 55.0%
TGGTTTGACCCGCAAGCCTA+TGG + chr1_3:51115206-51115225 Msa0123790:CDS 55.0%
GATGAGTGCCATAGGCTTGC+GGG - chr1_3:51115217-51115236 None:intergenic 55.0%
CATCAAGAAGCTCCGCAAGG+CGG + chr1_3:51115233-51115252 Msa0123790:CDS 55.0%
!! CAGAAAAGGGAAGCACCTCC+GGG + chr1_3:51115957-51115976 Msa0123790:intron 55.0%
!! ACAGGTTCTGGCTTCTCACC+CGG - chr1_3:51115978-51115997 None:intergenic 55.0%
GTGAGAAGCCAGAACCTGTG+AGG + chr1_3:51115979-51115998 Msa0123790:CDS 55.0%
AGGTGAGTCCTCACAGGTTC+TGG - chr1_3:51115990-51116009 None:intergenic 55.0%
TTACGGAGGTGAGTCCTCAC+AGG - chr1_3:51115996-51116015 None:intergenic 55.0%
!! TTGGTGCTACTCACTCCTCC+AGG + chr1_3:51116168-51116187 Msa0123790:CDS 55.0%
GCGGCTGCTTCTTACCTCTC+AGG - chr1_3:51114456-51114475 None:intergenic 60.0%
CCTGTTAAGCACGGTAGGCC+CGG + chr1_3:51116143-51116162 Msa0123790:CDS 60.0%
AAGCACGGTAGGCCCGGTAT+TGG + chr1_3:51116149-51116168 Msa0123790:CDS 60.0%
GCGGCTGCTTCTTACCTCTC+AGG - chr1_3:51114456-51114475 None:intergenic 60.0%
CCTGTTAAGCACGGTAGGCC+CGG + chr1_3:51116143-51116162 Msa0123790:CDS 60.0%
AAGCACGGTAGGCCCGGTAT+TGG + chr1_3:51116149-51116168 Msa0123790:CDS 60.0%
!! GGTTCTGGCTTCTCACCCGG+AGG - chr1_3:51115975-51115994 None:intergenic 65.0%
CCGGGCCTACCGTGCTTAAC+AGG - chr1_3:51116146-51116165 None:intergenic 65.0%
!! GGTTCTGGCTTCTCACCCGG+AGG - chr1_3:51115975-51115994 None:intergenic 65.0%
CCGGGCCTACCGTGCTTAAC+AGG - chr1_3:51116146-51116165 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 51114424 51116698 51114424 ID=Msa0123790;Name=Msa0123790
chr1_3 mRNA 51114424 51116698 51114424 ID=Msa0123790-mRNA-1;Parent=Msa0123790;Name=Msa0123790-mRNA-1;_AED=0.00;_eAED=0.00;_QI=88|1|1|1|0.75|0.6|5|390|152
chr1_3 exon 51114424 51114514 51114424 ID=Msa0123790-mRNA-1:exon:15613;Parent=Msa0123790-mRNA-1
chr1_3 exon 51114609 51114757 51114609 ID=Msa0123790-mRNA-1:exon:15614;Parent=Msa0123790-mRNA-1
chr1_3 exon 51115197 51115254 51115197 ID=Msa0123790-mRNA-1:exon:15615;Parent=Msa0123790-mRNA-1
chr1_3 exon 51115960 51116284 51115960 ID=Msa0123790-mRNA-1:exon:15616;Parent=Msa0123790-mRNA-1
chr1_3 exon 51116385 51116698 51116385 ID=Msa0123790-mRNA-1:exon:15617;Parent=Msa0123790-mRNA-1
chr1_3 five_prime_UTR 51114424 51114511 51114424 ID=Msa0123790-mRNA-1:five_prime_utr;Parent=Msa0123790-mRNA-1
chr1_3 CDS 51114512 51114514 51114512 ID=Msa0123790-mRNA-1:cds;Parent=Msa0123790-mRNA-1
chr1_3 CDS 51114609 51114757 51114609 ID=Msa0123790-mRNA-1:cds;Parent=Msa0123790-mRNA-1
chr1_3 CDS 51115197 51115254 51115197 ID=Msa0123790-mRNA-1:cds;Parent=Msa0123790-mRNA-1
chr1_3 CDS 51115960 51116208 51115960 ID=Msa0123790-mRNA-1:cds;Parent=Msa0123790-mRNA-1
chr1_3 three_prime_UTR 51116209 51116284 51116209 ID=Msa0123790-mRNA-1:three_prime_utr;Parent=Msa0123790-mRNA-1
chr1_3 three_prime_UTR 51116385 51116698 51116385 ID=Msa0123790-mRNA-1:three_prime_utr;Parent=Msa0123790-mRNA-1
Gene Sequence

>Msa0123790

ATGGCGGATGTTGAACCAGAGGTAGCTGCACAAGGAGTGCCAAAGAAGAGAACATTTAAGAAGTTCAGTTTCCGTGGTGTTGATCTCGATGCTCTTTTGGATATGTCCACTGATGAACTTGTCAAGCTTTTCTCTGCTCGTGCACGTAGAAGGTTTCAACGTGGTTTGACCCGCAAGCCTATGGCACTCATCAAGAAGCTCCGCAAGGCGAAAAGGGAAGCACCTCCGGGTGAGAAGCCAGAACCTGTGAGGACTCACCTCCGTAACATGATCATTGTGCCTGAGATGATCGGTAGCATTATTGGTGTCTACAATGGAAAGACCTTCAACCAAGTTGAAATTAAGCCTGAAATGATTGGCCACTATCTGGCTGAATTTTCAATCTCTTACAAGCCTGTTAAGCACGGTAGGCCCGGTATTGGTGCTACTCACTCCTCCAGGTTTATTCCTTTGAAGTGA
Protein sequence

>Msa0123790

MADVEPEVAAQGVPKKRTFKKFSFRGVDLDALLDMSTDELVKLFSARARRRFQRGLTRKPMALIKKLRKAKREAPPGEKPEPVRTHLRNMIIVPEMIGSIIGVYNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK*