AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0028150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0028150 MtrunA17_Chr1g0184681 99.342 152 1 0 1 152 1 152 2.95e-109 307
Msa0028150 MtrunA17_Chr4g0038481 96.711 152 5 0 1 152 1 152 8.07e-105 296
Msa0028150 MtrunA17_Chr8g0391601 96.104 154 4 1 1 152 1 154 1.61e-104 295
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0028150 AT5G09510.1 94.079 152 9 0 1 152 1 152 6.82e-96 273
Msa0028150 AT1G04270.1 94.079 152 9 0 1 152 1 152 1.06e-95 273
Msa0028150 AT5G09500.1 88.816 152 15 1 1 152 1 150 9.89e-95 270
Msa0028150 AT1G04270.2 93.421 152 9 1 1 152 1 151 3.95e-93 266
Msa0028150 AT5G09490.1 82.237 152 27 0 1 152 1 152 1.17e-90 260
Msa0028150 AT5G43640.1 87.919 149 18 0 4 152 1 149 4.43e-85 246
Msa0028150 AT5G09510.2 94.915 118 6 0 35 152 1 118 4.22e-71 209
Msa0028150 AT5G63070.1 62.500 160 52 3 1 152 1 160 3.97e-63 191
Msa0028150 AT1G33850.1 80.597 67 13 0 4 70 1 67 2.53e-22 84.7

Find 71 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTAAATGTTCTCTTCTT+TGG 0.218192 1_1:-52909338 None:intergenic
TGTTGATCTCGATGCTCTTT+TGG 0.301311 1_1:+52909376 Msa0028150:CDS
TTCCCAGCATATCTTTCATT+TGG 0.318679 1_1:-52911240 None:intergenic
AGGTGAGTCCTCACAGGTTC+TGG 0.319193 1_1:-52910694 None:intergenic
ATATCAGTGAACTAAAGTTT+TGG 0.345093 1_1:+52911023 Msa0028150:three_prime_UTR
GAGATGATCGGTAGCATTAT+TGG 0.345545 1_1:+52910739 Msa0028150:CDS
CCGGGCCTACCGTGCTTAAC+AGG 0.350740 1_1:-52910850 None:intergenic
AGATAGTGGCCAATCATTTC+AGG 0.354585 1_1:-52910802 None:intergenic
GATGAGTGCCATAGGCTTGC+GGG 0.362183 1_1:-52909920 None:intergenic
TGAAATGATTGGCCACTATC+TGG 0.368994 1_1:+52910804 Msa0028150:CDS
TCTCTGCTCGTGCACGTAGA+AGG 0.369916 1_1:+52909429 Msa0028150:CDS
ACTTAAATCCTGAGCGAATT+CGG 0.375078 1_1:+52911168 Msa0028150:three_prime_UTR
TATTTGGTTTATAGATAGAT+TGG 0.376185 1_1:+52911098 Msa0028150:three_prime_UTR
GAAATTAAGCCTGAAATGAT+TGG 0.379557 1_1:+52910793 Msa0028150:CDS
CTTAATTTCAACTTGGTTGA+AGG 0.392478 1_1:-52910779 None:intergenic
TACCTCTCAGGGATTGTGCT+AGG 0.395694 1_1:-52909134 None:intergenic
GCGGCTGCTTCTTACCTCTC+AGG 0.399037 1_1:-52909146 None:intergenic
ACAGAAAAGGGAAGCACCTC+CGG 0.413302 1_1:+52910663 Msa0028150:intron
ACTCCTTGTGCAGCTACCTC+TGG 0.435684 1_1:-52909314 None:intergenic
GCAGTGAAACTTGCTCGATC+TGG 0.436867 1_1:-52909178 None:intergenic
AGTCCAAATGAAAGATATGC+TGG 0.445961 1_1:+52911237 Msa0028150:three_prime_UTR
CGGCTGCTTCTTACCTCTCA+GGG 0.459104 1_1:-52909145 None:intergenic
ACCTCTCAGGGATTGTGCTA+GGG 0.474600 1_1:-52909133 None:intergenic
TATTATTGACCATGAAAGCT+TGG 0.476757 1_1:+52911122 Msa0028150:three_prime_UTR
CTTGTCTACCGAATTCGCTC+AGG 0.483917 1_1:-52911176 None:intergenic
TTGAAAATTCAGCCAGATAG+TGG 0.486704 1_1:-52910816 None:intergenic
TTGGTGCTACTCACTCCTCC+AGG 0.496949 1_1:+52910875 Msa0028150:CDS
TGGTTTGACCCGCAAGCCTA+TGG 0.497772 1_1:+52909912 Msa0028150:CDS
GGAGTGAGTAGCACCAATAC+CGG 0.503041 1_1:-52910869 None:intergenic
AGCGAATTCGGTAGACAAGT+TGG 0.504139 1_1:+52911180 Msa0028150:three_prime_UTR
AAGCACGGTAGGCCCGGTAT+TGG 0.508994 1_1:+52910856 Msa0028150:CDS
AATGACAATCTTCACTTCAA+AGG 0.512033 1_1:-52910904 None:intergenic
CCTGTTAAGCACGGTAGGCC+CGG 0.514282 1_1:+52910850 Msa0028150:CDS
CAGAAAAGGGAAGCACCTCC+GGG 0.515716 1_1:+52910664 Msa0028150:intron
ACAGGTTCTGGCTTCTCACC+CGG 0.516641 1_1:-52910682 None:intergenic
TGATGAGTGCCATAGGCTTG+CGG 0.517926 1_1:-52909921 None:intergenic
AGCAAGTTTCACTGCAATCA+TGG 0.522868 1_1:+52909186 Msa0028150:exon
GTTTGCATTGAAAGATAATG+TGG 0.529819 1_1:-52910933 None:intergenic
ATAATGCTACCGATCATCTC+AGG 0.534264 1_1:-52910736 None:intergenic
CAGGCACAATGATCATGTTA+CGG 0.535956 1_1:-52910717 None:intergenic
TTTCAGGCTTAATTTCAACT+TGG 0.556240 1_1:-52910786 None:intergenic
TTTGCATTGAAAGATAATGT+GGG 0.577878 1_1:-52910932 None:intergenic
AGCATTATTGGTGTCTACAA+TGG 0.579267 1_1:+52910751 Msa0028150:CDS
TTTAAGAAGTTCAGTTTCCG+TGG 0.586451 1_1:+52909353 Msa0028150:CDS
TCACTTCAAAGGAATAAACC+TGG 0.587310 1_1:-52910893 None:intergenic
TTACGGAGGTGAGTCCTCAC+AGG 0.595096 1_1:-52910700 None:intergenic
AGCTTCTTGATGAGTGCCAT+AGG 0.595409 1_1:-52909928 None:intergenic
ATAAACCAAATAAAATCAAG+AGG 0.595454 1_1:-52911087 None:intergenic
GTCCAAATGAAAGATATGCT+GGG 0.595723 1_1:+52911238 Msa0028150:three_prime_UTR
TGTATATATTCATGAACTAG+AGG 0.599285 1_1:+52911205 Msa0028150:three_prime_UTR
GAACCAGAGGTAGCTGCACA+AGG 0.605663 1_1:+52909311 Msa0028150:CDS
CTCGATCTGGTTCTTGCTCG+CGG 0.612106 1_1:-52909165 None:intergenic
GAGTGAGTAGCACCAATACC+GGG 0.613332 1_1:-52910868 None:intergenic
TGATATTGTTATGACACTAG+AGG 0.627040 1_1:+52911000 Msa0028150:three_prime_UTR
AAGCTTGACAAGTTCATCAG+TGG 0.629931 1_1:-52909405 None:intergenic
ACAAGCCTGTTAAGCACGGT+AGG 0.632440 1_1:+52910845 Msa0028150:CDS
TTGCATTGAAAGATAATGTG+GGG 0.633934 1_1:-52910931 None:intergenic
TCTTACAAGCCTGTTAAGCA+CGG 0.639372 1_1:+52910841 Msa0028150:CDS
TCAGGCGGATGTTGAACCAG+AGG 0.645787 1_1:+52909298 Msa0028150:intron
ACTCATCAAGAAGCTCCGCA+AGG 0.648877 1_1:+52909936 Msa0028150:CDS
ATCATTGTGCCTGAGATGAT+CGG 0.650070 1_1:+52910727 Msa0028150:CDS
GGTTCTGGCTTCTCACCCGG+AGG 0.654327 1_1:-52910679 None:intergenic
ACAGATAACTCACCGCCTTG+CGG 0.662337 1_1:-52909951 None:intergenic
TCCTTCACAATCCACAACAG+AGG 0.683089 1_1:+52909102 None:intergenic
GTGAGAAGCCAGAACCTGTG+AGG 0.700359 1_1:+52910686 Msa0028150:CDS
ACCCTAGCACAATCCCTGAG+AGG 0.707194 1_1:+52909132 Msa0028150:five_prime_UTR
GCACAATGATCATGTTACGG+AGG 0.711118 1_1:-52910714 None:intergenic
CTTCAAAGGAATAAACCTGG+AGG 0.714921 1_1:-52910890 None:intergenic
CATCAAGAAGCTCCGCAAGG+CGG 0.735053 1_1:+52909939 Msa0028150:CDS
GAGCATCGAGATCAACACCA+CGG 0.739088 1_1:-52909370 None:intergenic
GTTCATTTCAGGTTTCAACG+TGG 0.745899 1_1:+52909892 Msa0028150:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCATTTTGTTTTATTTTG+TGG + chr1_1:52909674-52909693 Msa0028150:intron 15.0%
!!! TTCATTTTGTTTTATTTTGT+GGG + chr1_1:52909675-52909694 Msa0028150:intron 15.0%
!!! TTTTATATTCTGTTACTTAT+AGG + chr1_1:52909800-52909819 Msa0028150:intron 15.0%
!!! TTTCATTTTGTTTTATTTTG+TGG + chr1_1:52909674-52909693 Msa0028150:intron 15.0%
!!! TTCATTTTGTTTTATTTTGT+GGG + chr1_1:52909675-52909694 Msa0028150:intron 15.0%
!!! TTTTATATTCTGTTACTTAT+AGG + chr1_1:52909800-52909819 Msa0028150:intron 15.0%
!!! ATGAATTTGATTGATTTTTC+AGG + chr1_1:52909280-52909299 Msa0028150:intron 20.0%
!! TAACCTAAAAATGATGAAAA+TGG - chr1_1:52909620-52909639 None:intergenic 20.0%
!!! TTCATCATTTTTAGGTTATT+TGG + chr1_1:52909622-52909641 Msa0028150:intron 20.0%
!! AACCTAAAATATTCAACATT+TGG - chr1_1:52909648-52909667 None:intergenic 20.0%
!! TTTAACAATATGTTCATTTC+AGG + chr1_1:52909881-52909900 Msa0028150:intron 20.0%
!! TTTGGAAATTGTTTATTTAG+CGG + chr1_1:52910088-52910107 Msa0028150:intron 20.0%
!! ACAATATACTGTCTTAAATT+TGG + chr1_1:52910311-52910330 Msa0028150:intron 20.0%
!! CAATATACTGTCTTAAATTT+GGG + chr1_1:52910312-52910331 Msa0028150:intron 20.0%
!! AATATACTGTCTTAAATTTG+GGG + chr1_1:52910313-52910332 Msa0028150:intron 20.0%
!!! TTTAAAATGATAGCATGATT+GGG - chr1_1:52910566-52910585 None:intergenic 20.0%
!!! ATTTAAAATGATAGCATGAT+TGG - chr1_1:52910567-52910586 None:intergenic 20.0%
!! TTGTATATTGTTACAGAAAA+GGG + chr1_1:52910651-52910670 Msa0028150:intron 20.0%
!! ATAAACCAAATAAAATCAAG+AGG - chr1_1:52911090-52911109 None:intergenic 20.0%
!!! TATTTGGTTTATAGATAGAT+TGG + chr1_1:52911098-52911117 Msa0028150:three_prime_UTR 20.0%
!!! ATGAATTTGATTGATTTTTC+AGG + chr1_1:52909280-52909299 Msa0028150:intron 20.0%
!! TAACCTAAAAATGATGAAAA+TGG - chr1_1:52909620-52909639 None:intergenic 20.0%
!!! TTCATCATTTTTAGGTTATT+TGG + chr1_1:52909622-52909641 Msa0028150:intron 20.0%
!! AACCTAAAATATTCAACATT+TGG - chr1_1:52909648-52909667 None:intergenic 20.0%
!! TTTAACAATATGTTCATTTC+AGG + chr1_1:52909881-52909900 Msa0028150:intron 20.0%
!! TTTGGAAATTGTTTATTTAG+CGG + chr1_1:52910088-52910107 Msa0028150:intron 20.0%
!! ACAATATACTGTCTTAAATT+TGG + chr1_1:52910311-52910330 Msa0028150:intron 20.0%
!! CAATATACTGTCTTAAATTT+GGG + chr1_1:52910312-52910331 Msa0028150:intron 20.0%
!! AATATACTGTCTTAAATTTG+GGG + chr1_1:52910313-52910332 Msa0028150:intron 20.0%
!!! TTTAAAATGATAGCATGATT+GGG - chr1_1:52910566-52910585 None:intergenic 20.0%
!!! ATTTAAAATGATAGCATGAT+TGG - chr1_1:52910567-52910586 None:intergenic 20.0%
!! TTGTATATTGTTACAGAAAA+GGG + chr1_1:52910651-52910670 Msa0028150:intron 20.0%
!! ATAAACCAAATAAAATCAAG+AGG - chr1_1:52911090-52911109 None:intergenic 20.0%
!!! TATTTGGTTTATAGATAGAT+TGG + chr1_1:52911098-52911117 Msa0028150:three_prime_UTR 20.0%
!!! AATTTGATTGATTTTTCAGG+CGG + chr1_1:52909283-52909302 Msa0028150:intron 25.0%
! TTCTTAAATGTTCTCTTCTT+TGG - chr1_1:52909341-52909360 None:intergenic 25.0%
! GAATATATGAATGATGAAGA+TGG + chr1_1:52909510-52909529 Msa0028150:intron 25.0%
!!! CGTTAATCTGATTTATTTTG+AGG + chr1_1:52909560-52909579 Msa0028150:intron 25.0%
! GAAATTAACACAAATCCTTA+AGG - chr1_1:52909828-52909847 None:intergenic 25.0%
! GTAAATGTGAAAATGAGAAA+TGG - chr1_1:52909860-52909879 None:intergenic 25.0%
!! ATGTAATTGATTCATTTCAG+TGG + chr1_1:52910009-52910028 Msa0028150:intron 25.0%
!! ATTCTAGTTGTTGAGTAATT+TGG + chr1_1:52910070-52910089 Msa0028150:intron 25.0%
! CGTTAATCTGATTTATTATG+AGG + chr1_1:52910211-52910230 Msa0028150:intron 25.0%
! AAAGTAATGAATGGATATGA+AGG - chr1_1:52909590-52909609 None:intergenic 25.0%
!!! ATGTTTTAGATCAAGTAGAA+GGG - chr1_1:52910271-52910290 None:intergenic 25.0%
! ATATACTGTCTTAAATTTGG+GGG + chr1_1:52910314-52910333 Msa0028150:intron 25.0%
! TGTATGATTATGTATCTAAC+TGG + chr1_1:52910463-52910482 Msa0028150:intron 25.0%
!!! ATTTTGGGTACATAATCATA+CGG - chr1_1:52910602-52910621 None:intergenic 25.0%
!! AACACCTAACAACAAATTTT+GGG - chr1_1:52910617-52910636 None:intergenic 25.0%
! GTTGTATATTGTTACAGAAA+AGG + chr1_1:52910650-52910669 Msa0028150:intron 25.0%
! TTTGCATTGAAAGATAATGT+GGG - chr1_1:52910935-52910954 None:intergenic 25.0%
!! ATATCAGTGAACTAAAGTTT+TGG + chr1_1:52911023-52911042 Msa0028150:three_prime_UTR 25.0%
!! TATCAGTGAACTAAAGTTTT+GGG + chr1_1:52911024-52911043 Msa0028150:three_prime_UTR 25.0%
!! TGTTTCCTCTTGATTTTATT+TGG + chr1_1:52911082-52911101 Msa0028150:three_prime_UTR 25.0%
!!! TAATTTTTTCCAAGCTTTCA+TGG - chr1_1:52911134-52911153 None:intergenic 25.0%
! AAGCTTGGAAAAAATTATTG+TGG + chr1_1:52911137-52911156 Msa0028150:three_prime_UTR 25.0%
! AGCTTGGAAAAAATTATTGT+GGG + chr1_1:52911138-52911157 Msa0028150:three_prime_UTR 25.0%
! TGTATATATTCATGAACTAG+AGG + chr1_1:52911205-52911224 Msa0028150:three_prime_UTR 25.0%
!!! AATTTGATTGATTTTTCAGG+CGG + chr1_1:52909283-52909302 Msa0028150:intron 25.0%
! TTCTTAAATGTTCTCTTCTT+TGG - chr1_1:52909341-52909360 None:intergenic 25.0%
! GAATATATGAATGATGAAGA+TGG + chr1_1:52909510-52909529 Msa0028150:intron 25.0%
!!! CGTTAATCTGATTTATTTTG+AGG + chr1_1:52909560-52909579 Msa0028150:intron 25.0%
! GAAATTAACACAAATCCTTA+AGG - chr1_1:52909828-52909847 None:intergenic 25.0%
! GTAAATGTGAAAATGAGAAA+TGG - chr1_1:52909860-52909879 None:intergenic 25.0%
!! ATGTAATTGATTCATTTCAG+TGG + chr1_1:52910009-52910028 Msa0028150:intron 25.0%
!! ATTCTAGTTGTTGAGTAATT+TGG + chr1_1:52910070-52910089 Msa0028150:intron 25.0%
! CGTTAATCTGATTTATTATG+AGG + chr1_1:52910211-52910230 Msa0028150:intron 25.0%
! AAAGTAATGAATGGATATGA+AGG - chr1_1:52909590-52909609 None:intergenic 25.0%
!!! ATGTTTTAGATCAAGTAGAA+GGG - chr1_1:52910271-52910290 None:intergenic 25.0%
! ATATACTGTCTTAAATTTGG+GGG + chr1_1:52910314-52910333 Msa0028150:intron 25.0%
! TGTATGATTATGTATCTAAC+TGG + chr1_1:52910463-52910482 Msa0028150:intron 25.0%
!!! ATTTTGGGTACATAATCATA+CGG - chr1_1:52910602-52910621 None:intergenic 25.0%
!! AACACCTAACAACAAATTTT+GGG - chr1_1:52910617-52910636 None:intergenic 25.0%
! GTTGTATATTGTTACAGAAA+AGG + chr1_1:52910650-52910669 Msa0028150:intron 25.0%
! TTTGCATTGAAAGATAATGT+GGG - chr1_1:52910935-52910954 None:intergenic 25.0%
!! ATATCAGTGAACTAAAGTTT+TGG + chr1_1:52911023-52911042 Msa0028150:three_prime_UTR 25.0%
!! TATCAGTGAACTAAAGTTTT+GGG + chr1_1:52911024-52911043 Msa0028150:three_prime_UTR 25.0%
!! TGTTTCCTCTTGATTTTATT+TGG + chr1_1:52911082-52911101 Msa0028150:three_prime_UTR 25.0%
!!! TAATTTTTTCCAAGCTTTCA+TGG - chr1_1:52911134-52911153 None:intergenic 25.0%
! AAGCTTGGAAAAAATTATTG+TGG + chr1_1:52911137-52911156 Msa0028150:three_prime_UTR 25.0%
! AGCTTGGAAAAAATTATTGT+GGG + chr1_1:52911138-52911157 Msa0028150:three_prime_UTR 25.0%
! TGTATATATTCATGAACTAG+AGG + chr1_1:52911205-52911224 Msa0028150:three_prime_UTR 25.0%
AAGAGAAAGAGATAGAGTTA+GGG - chr1_1:52909218-52909237 None:intergenic 30.0%
!! GCACCATTTTCATCATTTTT+AGG + chr1_1:52909614-52909633 Msa0028150:intron 30.0%
! GGCCAAATGTTGAATATTTT+AGG + chr1_1:52909643-52909662 Msa0028150:intron 30.0%
!! TAGCATTTTGAAGAAATGTG+AGG + chr1_1:52909727-52909746 Msa0028150:intron 30.0%
! TGTTACTTATAGGATCCTTA+AGG + chr1_1:52909810-52909829 Msa0028150:intron 30.0%
GAAATTGTTTATTTAGCGGT+TGG + chr1_1:52910092-52910111 Msa0028150:intron 30.0%
! TCTTTTGCGTTTGTATCTTT+TGG + chr1_1:52910160-52910179 Msa0028150:intron 30.0%
! CTTTTGCGTTTGTATCTTTT+GGG + chr1_1:52910161-52910180 Msa0028150:intron 30.0%
!! GATGTTTTAGATCAAGTAGA+AGG - chr1_1:52910272-52910291 None:intergenic 30.0%
TATACTGTCTTAAATTTGGG+GGG + chr1_1:52910315-52910334 Msa0028150:intron 30.0%
GCATTTGAACTTAACAGATA+TGG + chr1_1:52910341-52910360 Msa0028150:intron 30.0%
ATCTCCTTTACAGATACATT+CGG + chr1_1:52910372-52910391 Msa0028150:intron 30.0%
!! TGTACCCAAAATTTGTTGTT+AGG + chr1_1:52910610-52910629 Msa0028150:intron 30.0%
! GAACACCTAACAACAAATTT+TGG - chr1_1:52910618-52910637 None:intergenic 30.0%
CTTAATTTCAACTTGGTTGA+AGG - chr1_1:52910782-52910801 None:intergenic 30.0%
TTTCAGGCTTAATTTCAACT+TGG - chr1_1:52910789-52910808 None:intergenic 30.0%
GAAATTAAGCCTGAAATGAT+TGG + chr1_1:52910793-52910812 Msa0028150:CDS 30.0%
AATGACAATCTTCACTTCAA+AGG - chr1_1:52910907-52910926 None:intergenic 30.0%
TTGCATTGAAAGATAATGTG+GGG - chr1_1:52910934-52910953 None:intergenic 30.0%
GTTTGCATTGAAAGATAATG+TGG - chr1_1:52910936-52910955 None:intergenic 30.0%
!!! CAAACTTTTGCTTGTTTTAC+TGG + chr1_1:52910951-52910970 Msa0028150:three_prime_UTR 30.0%
TGATATTGTTATGACACTAG+AGG + chr1_1:52911000-52911019 Msa0028150:three_prime_UTR 30.0%
! TTGTCTATTGATGAGCTTTT+TGG + chr1_1:52911053-52911072 Msa0028150:three_prime_UTR 30.0%
TATTATTGACCATGAAAGCT+TGG + chr1_1:52911122-52911141 Msa0028150:three_prime_UTR 30.0%
AAGAGAAAGAGATAGAGTTA+GGG - chr1_1:52909218-52909237 None:intergenic 30.0%
!! GCACCATTTTCATCATTTTT+AGG + chr1_1:52909614-52909633 Msa0028150:intron 30.0%
! GGCCAAATGTTGAATATTTT+AGG + chr1_1:52909643-52909662 Msa0028150:intron 30.0%
!! TAGCATTTTGAAGAAATGTG+AGG + chr1_1:52909727-52909746 Msa0028150:intron 30.0%
! TGTTACTTATAGGATCCTTA+AGG + chr1_1:52909810-52909829 Msa0028150:intron 30.0%
GAAATTGTTTATTTAGCGGT+TGG + chr1_1:52910092-52910111 Msa0028150:intron 30.0%
! TCTTTTGCGTTTGTATCTTT+TGG + chr1_1:52910160-52910179 Msa0028150:intron 30.0%
! CTTTTGCGTTTGTATCTTTT+GGG + chr1_1:52910161-52910180 Msa0028150:intron 30.0%
!! GATGTTTTAGATCAAGTAGA+AGG - chr1_1:52910272-52910291 None:intergenic 30.0%
TATACTGTCTTAAATTTGGG+GGG + chr1_1:52910315-52910334 Msa0028150:intron 30.0%
GCATTTGAACTTAACAGATA+TGG + chr1_1:52910341-52910360 Msa0028150:intron 30.0%
ATCTCCTTTACAGATACATT+CGG + chr1_1:52910372-52910391 Msa0028150:intron 30.0%
!! TGTACCCAAAATTTGTTGTT+AGG + chr1_1:52910610-52910629 Msa0028150:intron 30.0%
! GAACACCTAACAACAAATTT+TGG - chr1_1:52910618-52910637 None:intergenic 30.0%
CTTAATTTCAACTTGGTTGA+AGG - chr1_1:52910782-52910801 None:intergenic 30.0%
TTTCAGGCTTAATTTCAACT+TGG - chr1_1:52910789-52910808 None:intergenic 30.0%
GAAATTAAGCCTGAAATGAT+TGG + chr1_1:52910793-52910812 Msa0028150:CDS 30.0%
AATGACAATCTTCACTTCAA+AGG - chr1_1:52910907-52910926 None:intergenic 30.0%
TTGCATTGAAAGATAATGTG+GGG - chr1_1:52910934-52910953 None:intergenic 30.0%
GTTTGCATTGAAAGATAATG+TGG - chr1_1:52910936-52910955 None:intergenic 30.0%
!!! CAAACTTTTGCTTGTTTTAC+TGG + chr1_1:52910951-52910970 Msa0028150:three_prime_UTR 30.0%
TGATATTGTTATGACACTAG+AGG + chr1_1:52911000-52911019 Msa0028150:three_prime_UTR 30.0%
! TTGTCTATTGATGAGCTTTT+TGG + chr1_1:52911053-52911072 Msa0028150:three_prime_UTR 30.0%
TATTATTGACCATGAAAGCT+TGG + chr1_1:52911122-52911141 Msa0028150:three_prime_UTR 30.0%
GAAGAGAAAGAGATAGAGTT+AGG - chr1_1:52909219-52909238 None:intergenic 35.0%
TGCGAAATTCAAAGTAGAAG+AGG - chr1_1:52909250-52909269 None:intergenic 35.0%
TTTAAGAAGTTCAGTTTCCG+TGG + chr1_1:52909353-52909372 Msa0028150:CDS 35.0%
TGTATCTAACTGGTTGTGTT+AGG + chr1_1:52910473-52910492 Msa0028150:intron 35.0%
! AGCATTATTGGTGTCTACAA+TGG + chr1_1:52910751-52910770 Msa0028150:CDS 35.0%
TTGAAAATTCAGCCAGATAG+TGG - chr1_1:52910819-52910838 None:intergenic 35.0%
TCACTTCAAAGGAATAAACC+TGG - chr1_1:52910896-52910915 None:intergenic 35.0%
ACTTAAATCCTGAGCGAATT+CGG + chr1_1:52911168-52911187 Msa0028150:three_prime_UTR 35.0%
AGTCCAAATGAAAGATATGC+TGG + chr1_1:52911237-52911256 Msa0028150:three_prime_UTR 35.0%
GTCCAAATGAAAGATATGCT+GGG + chr1_1:52911238-52911257 Msa0028150:three_prime_UTR 35.0%
TTCCCAGCATATCTTTCATT+TGG - chr1_1:52911243-52911262 None:intergenic 35.0%
GAAGAGAAAGAGATAGAGTT+AGG - chr1_1:52909219-52909238 None:intergenic 35.0%
TGCGAAATTCAAAGTAGAAG+AGG - chr1_1:52909250-52909269 None:intergenic 35.0%
TTTAAGAAGTTCAGTTTCCG+TGG + chr1_1:52909353-52909372 Msa0028150:CDS 35.0%
TGTATCTAACTGGTTGTGTT+AGG + chr1_1:52910473-52910492 Msa0028150:intron 35.0%
! AGCATTATTGGTGTCTACAA+TGG + chr1_1:52910751-52910770 Msa0028150:CDS 35.0%
TTGAAAATTCAGCCAGATAG+TGG - chr1_1:52910819-52910838 None:intergenic 35.0%
TCACTTCAAAGGAATAAACC+TGG - chr1_1:52910896-52910915 None:intergenic 35.0%
ACTTAAATCCTGAGCGAATT+CGG + chr1_1:52911168-52911187 Msa0028150:three_prime_UTR 35.0%
AGTCCAAATGAAAGATATGC+TGG + chr1_1:52911237-52911256 Msa0028150:three_prime_UTR 35.0%
GTCCAAATGAAAGATATGCT+GGG + chr1_1:52911238-52911257 Msa0028150:three_prime_UTR 35.0%
TTCCCAGCATATCTTTCATT+TGG - chr1_1:52911243-52911262 None:intergenic 35.0%
AGCAAGTTTCACTGCAATCA+TGG + chr1_1:52909186-52909205 Msa0028150:exon 40.0%
! TGTTGATCTCGATGCTCTTT+TGG + chr1_1:52909376-52909395 Msa0028150:CDS 40.0%
AAGCTTGACAAGTTCATCAG+TGG - chr1_1:52909408-52909427 None:intergenic 40.0%
GGTGCTCAGAAAGTAATGAA+TGG - chr1_1:52909599-52909618 None:intergenic 40.0%
!! GTTCATTTCAGGTTTCAACG+TGG + chr1_1:52909892-52909911 Msa0028150:intron 40.0%
GGTGCTCAGAAAGTAATGAA+TGG - chr1_1:52909599-52909618 None:intergenic 40.0%
CAGGCACAATGATCATGTTA+CGG - chr1_1:52910720-52910739 None:intergenic 40.0%
ATCATTGTGCCTGAGATGAT+CGG + chr1_1:52910727-52910746 Msa0028150:CDS 40.0%
ATAATGCTACCGATCATCTC+AGG - chr1_1:52910739-52910758 None:intergenic 40.0%
GAGATGATCGGTAGCATTAT+TGG + chr1_1:52910739-52910758 Msa0028150:CDS 40.0%
! AGATAGTGGCCAATCATTTC+AGG - chr1_1:52910805-52910824 None:intergenic 40.0%
! TGAAATGATTGGCCACTATC+TGG + chr1_1:52910804-52910823 Msa0028150:CDS 40.0%
TCTTACAAGCCTGTTAAGCA+CGG + chr1_1:52910841-52910860 Msa0028150:CDS 40.0%
CTTCAAAGGAATAAACCTGG+AGG - chr1_1:52910893-52910912 None:intergenic 40.0%
AGCAAGTTTCACTGCAATCA+TGG + chr1_1:52909186-52909205 Msa0028150:exon 40.0%
! TGTTGATCTCGATGCTCTTT+TGG + chr1_1:52909376-52909395 Msa0028150:CDS 40.0%
AAGCTTGACAAGTTCATCAG+TGG - chr1_1:52909408-52909427 None:intergenic 40.0%
GGTGCTCAGAAAGTAATGAA+TGG - chr1_1:52909599-52909618 None:intergenic 40.0%
!! GTTCATTTCAGGTTTCAACG+TGG + chr1_1:52909892-52909911 Msa0028150:intron 40.0%
GGTGCTCAGAAAGTAATGAA+TGG - chr1_1:52909599-52909618 None:intergenic 40.0%
CAGGCACAATGATCATGTTA+CGG - chr1_1:52910720-52910739 None:intergenic 40.0%
ATCATTGTGCCTGAGATGAT+CGG + chr1_1:52910727-52910746 Msa0028150:CDS 40.0%
ATAATGCTACCGATCATCTC+AGG - chr1_1:52910739-52910758 None:intergenic 40.0%
GAGATGATCGGTAGCATTAT+TGG + chr1_1:52910739-52910758 Msa0028150:CDS 40.0%
! AGATAGTGGCCAATCATTTC+AGG - chr1_1:52910805-52910824 None:intergenic 40.0%
! TGAAATGATTGGCCACTATC+TGG + chr1_1:52910804-52910823 Msa0028150:CDS 40.0%
TCTTACAAGCCTGTTAAGCA+CGG + chr1_1:52910841-52910860 Msa0028150:CDS 40.0%
CTTCAAAGGAATAAACCTGG+AGG - chr1_1:52910893-52910912 None:intergenic 40.0%
AGCTTCTTGATGAGTGCCAT+AGG - chr1_1:52909931-52909950 None:intergenic 45.0%
GCAGCCGAATGTATCTGTAA+AGG - chr1_1:52910379-52910398 None:intergenic 45.0%
GCACAATGATCATGTTACGG+AGG - chr1_1:52910717-52910736 None:intergenic 45.0%
AGCGAATTCGGTAGACAAGT+TGG + chr1_1:52911180-52911199 Msa0028150:three_prime_UTR 45.0%
AGCTTCTTGATGAGTGCCAT+AGG - chr1_1:52909931-52909950 None:intergenic 45.0%
GCAGCCGAATGTATCTGTAA+AGG - chr1_1:52910379-52910398 None:intergenic 45.0%
GCACAATGATCATGTTACGG+AGG - chr1_1:52910717-52910736 None:intergenic 45.0%
AGCGAATTCGGTAGACAAGT+TGG + chr1_1:52911180-52911199 Msa0028150:three_prime_UTR 45.0%
ACCTCTCAGGGATTGTGCTA+GGG - chr1_1:52909136-52909155 None:intergenic 50.0%
TACCTCTCAGGGATTGTGCT+AGG - chr1_1:52909137-52909156 None:intergenic 50.0%
! GCAGTGAAACTTGCTCGATC+TGG - chr1_1:52909181-52909200 None:intergenic 50.0%
!! GAGCATCGAGATCAACACCA+CGG - chr1_1:52909373-52909392 None:intergenic 50.0%
TGATGAGTGCCATAGGCTTG+CGG - chr1_1:52909924-52909943 None:intergenic 50.0%
ACTCATCAAGAAGCTCCGCA+AGG + chr1_1:52909936-52909955 Msa0028150:CDS 50.0%
ACAGATAACTCACCGCCTTG+CGG - chr1_1:52909954-52909973 None:intergenic 50.0%
!! ACAGAAAAGGGAAGCACCTC+CGG + chr1_1:52910663-52910682 Msa0028150:intron 50.0%
ACAAGCCTGTTAAGCACGGT+AGG + chr1_1:52910845-52910864 Msa0028150:CDS 50.0%
GAGTGAGTAGCACCAATACC+GGG - chr1_1:52910871-52910890 None:intergenic 50.0%
GGAGTGAGTAGCACCAATAC+CGG - chr1_1:52910872-52910891 None:intergenic 50.0%
CTTGTCTACCGAATTCGCTC+AGG - chr1_1:52911179-52911198 None:intergenic 50.0%
ACCTCTCAGGGATTGTGCTA+GGG - chr1_1:52909136-52909155 None:intergenic 50.0%
TACCTCTCAGGGATTGTGCT+AGG - chr1_1:52909137-52909156 None:intergenic 50.0%
! GCAGTGAAACTTGCTCGATC+TGG - chr1_1:52909181-52909200 None:intergenic 50.0%
!! GAGCATCGAGATCAACACCA+CGG - chr1_1:52909373-52909392 None:intergenic 50.0%
TGATGAGTGCCATAGGCTTG+CGG - chr1_1:52909924-52909943 None:intergenic 50.0%
ACTCATCAAGAAGCTCCGCA+AGG + chr1_1:52909936-52909955 Msa0028150:CDS 50.0%
ACAGATAACTCACCGCCTTG+CGG - chr1_1:52909954-52909973 None:intergenic 50.0%
!! ACAGAAAAGGGAAGCACCTC+CGG + chr1_1:52910663-52910682 Msa0028150:intron 50.0%
ACAAGCCTGTTAAGCACGGT+AGG + chr1_1:52910845-52910864 Msa0028150:CDS 50.0%
GAGTGAGTAGCACCAATACC+GGG - chr1_1:52910871-52910890 None:intergenic 50.0%
GGAGTGAGTAGCACCAATAC+CGG - chr1_1:52910872-52910891 None:intergenic 50.0%
CTTGTCTACCGAATTCGCTC+AGG - chr1_1:52911179-52911198 None:intergenic 50.0%
ACCCTAGCACAATCCCTGAG+AGG + chr1_1:52909132-52909151 Msa0028150:five_prime_UTR 55.0%
CGGCTGCTTCTTACCTCTCA+GGG - chr1_1:52909148-52909167 None:intergenic 55.0%
!! CTCGATCTGGTTCTTGCTCG+CGG - chr1_1:52909168-52909187 None:intergenic 55.0%
TCAGGCGGATGTTGAACCAG+AGG + chr1_1:52909298-52909317 Msa0028150:intron 55.0%
GAACCAGAGGTAGCTGCACA+AGG + chr1_1:52909311-52909330 Msa0028150:CDS 55.0%
ACTCCTTGTGCAGCTACCTC+TGG - chr1_1:52909317-52909336 None:intergenic 55.0%
TCTCTGCTCGTGCACGTAGA+AGG + chr1_1:52909429-52909448 Msa0028150:CDS 55.0%
TGGTTTGACCCGCAAGCCTA+TGG + chr1_1:52909912-52909931 Msa0028150:CDS 55.0%
GATGAGTGCCATAGGCTTGC+GGG - chr1_1:52909923-52909942 None:intergenic 55.0%
CATCAAGAAGCTCCGCAAGG+CGG + chr1_1:52909939-52909958 Msa0028150:CDS 55.0%
!! CAGAAAAGGGAAGCACCTCC+GGG + chr1_1:52910664-52910683 Msa0028150:intron 55.0%
!! ACAGGTTCTGGCTTCTCACC+CGG - chr1_1:52910685-52910704 None:intergenic 55.0%
GTGAGAAGCCAGAACCTGTG+AGG + chr1_1:52910686-52910705 Msa0028150:CDS 55.0%
AGGTGAGTCCTCACAGGTTC+TGG - chr1_1:52910697-52910716 None:intergenic 55.0%
TTACGGAGGTGAGTCCTCAC+AGG - chr1_1:52910703-52910722 None:intergenic 55.0%
!! TTGGTGCTACTCACTCCTCC+AGG + chr1_1:52910875-52910894 Msa0028150:CDS 55.0%
ACCCTAGCACAATCCCTGAG+AGG + chr1_1:52909132-52909151 Msa0028150:five_prime_UTR 55.0%
CGGCTGCTTCTTACCTCTCA+GGG - chr1_1:52909148-52909167 None:intergenic 55.0%
!! CTCGATCTGGTTCTTGCTCG+CGG - chr1_1:52909168-52909187 None:intergenic 55.0%
TCAGGCGGATGTTGAACCAG+AGG + chr1_1:52909298-52909317 Msa0028150:intron 55.0%
GAACCAGAGGTAGCTGCACA+AGG + chr1_1:52909311-52909330 Msa0028150:CDS 55.0%
ACTCCTTGTGCAGCTACCTC+TGG - chr1_1:52909317-52909336 None:intergenic 55.0%
TCTCTGCTCGTGCACGTAGA+AGG + chr1_1:52909429-52909448 Msa0028150:CDS 55.0%
TGGTTTGACCCGCAAGCCTA+TGG + chr1_1:52909912-52909931 Msa0028150:CDS 55.0%
GATGAGTGCCATAGGCTTGC+GGG - chr1_1:52909923-52909942 None:intergenic 55.0%
CATCAAGAAGCTCCGCAAGG+CGG + chr1_1:52909939-52909958 Msa0028150:CDS 55.0%
!! CAGAAAAGGGAAGCACCTCC+GGG + chr1_1:52910664-52910683 Msa0028150:intron 55.0%
!! ACAGGTTCTGGCTTCTCACC+CGG - chr1_1:52910685-52910704 None:intergenic 55.0%
GTGAGAAGCCAGAACCTGTG+AGG + chr1_1:52910686-52910705 Msa0028150:CDS 55.0%
AGGTGAGTCCTCACAGGTTC+TGG - chr1_1:52910697-52910716 None:intergenic 55.0%
TTACGGAGGTGAGTCCTCAC+AGG - chr1_1:52910703-52910722 None:intergenic 55.0%
!! TTGGTGCTACTCACTCCTCC+AGG + chr1_1:52910875-52910894 Msa0028150:CDS 55.0%
GCGGCTGCTTCTTACCTCTC+AGG - chr1_1:52909149-52909168 None:intergenic 60.0%
CCTGTTAAGCACGGTAGGCC+CGG + chr1_1:52910850-52910869 Msa0028150:CDS 60.0%
AAGCACGGTAGGCCCGGTAT+TGG + chr1_1:52910856-52910875 Msa0028150:CDS 60.0%
GCGGCTGCTTCTTACCTCTC+AGG - chr1_1:52909149-52909168 None:intergenic 60.0%
CCTGTTAAGCACGGTAGGCC+CGG + chr1_1:52910850-52910869 Msa0028150:CDS 60.0%
AAGCACGGTAGGCCCGGTAT+TGG + chr1_1:52910856-52910875 Msa0028150:CDS 60.0%
!! GGTTCTGGCTTCTCACCCGG+AGG - chr1_1:52910682-52910701 None:intergenic 65.0%
CCGGGCCTACCGTGCTTAAC+AGG - chr1_1:52910853-52910872 None:intergenic 65.0%
!! GGTTCTGGCTTCTCACCCGG+AGG - chr1_1:52910682-52910701 None:intergenic 65.0%
CCGGGCCTACCGTGCTTAAC+AGG - chr1_1:52910853-52910872 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 52909117 52911305 52909117 ID=Msa0028150;Name=Msa0028150
chr1_1 mRNA 52909117 52911305 52909117 ID=Msa0028150-mRNA-1;Parent=Msa0028150;Name=Msa0028150-mRNA-1;_AED=0.00;_eAED=0.00;_QI=88|1|1|1|0.66|0.75|4|390|152
chr1_1 exon 52909117 52909207 52909117 ID=Msa0028150-mRNA-1:exon:15556;Parent=Msa0028150-mRNA-1
chr1_1 exon 52909302 52909450 52909302 ID=Msa0028150-mRNA-1:exon:15557;Parent=Msa0028150-mRNA-1
chr1_1 exon 52909903 52909960 52909903 ID=Msa0028150-mRNA-1:exon:15558;Parent=Msa0028150-mRNA-1
chr1_1 exon 52910667 52911305 52910667 ID=Msa0028150-mRNA-1:exon:15559;Parent=Msa0028150-mRNA-1
chr1_1 five_prime_UTR 52909117 52909204 52909117 ID=Msa0028150-mRNA-1:five_prime_utr;Parent=Msa0028150-mRNA-1
chr1_1 CDS 52909205 52909207 52909205 ID=Msa0028150-mRNA-1:cds;Parent=Msa0028150-mRNA-1
chr1_1 CDS 52909302 52909450 52909302 ID=Msa0028150-mRNA-1:cds;Parent=Msa0028150-mRNA-1
chr1_1 CDS 52909903 52909960 52909903 ID=Msa0028150-mRNA-1:cds;Parent=Msa0028150-mRNA-1
chr1_1 CDS 52910667 52910915 52910667 ID=Msa0028150-mRNA-1:cds;Parent=Msa0028150-mRNA-1
chr1_1 three_prime_UTR 52910916 52911305 52910916 ID=Msa0028150-mRNA-1:three_prime_utr;Parent=Msa0028150-mRNA-1
Gene Sequence

>Msa0028150

ATGGCGGATGTTGAACCAGAGGTAGCTGCACAAGGAGTGCCAAAGAAGAGAACATTTAAGAAGTTCAGTTTCCGTGGTGTTGATCTCGATGCTCTTTTGGATATGTCCACTGATGAACTTGTCAAGCTTTTCTCTGCTCGTGCACGTAGAAGGTTTCAACGTGGTTTGACCCGCAAGCCTATGGCACTCATCAAGAAGCTCCGCAAGGCGAAAAGGGAAGCACCTCCGGGTGAGAAGCCAGAACCTGTGAGGACTCACCTCCGTAACATGATCATTGTGCCTGAGATGATCGGTAGCATTATTGGTGTCTACAATGGAAAGACCTTCAACCAAGTTGAAATTAAGCCTGAAATGATTGGCCACTATCTGGCTGAATTTTCAATCTCTTACAAGCCTGTTAAGCACGGTAGGCCCGGTATTGGTGCTACTCACTCCTCCAGGTTTATTCCTTTGAAGTGA
Protein sequence

>Msa0028150

MADVEPEVAAQGVPKKRTFKKFSFRGVDLDALLDMSTDELVKLFSARARRRFQRGLTRKPMALIKKLRKAKREAPPGEKPEPVRTHLRNMIIVPEMIGSIIGVYNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK*