Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0078190 | XP_013468806.1 | 94.353 | 726 | 22 | 1 | 1 | 726 | 1 | 707 | 0.0 | 1414 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0078190 | sp|Q9C810|Y1342_ARATH | 48.338 | 722 | 312 | 19 | 2 | 712 | 3 | 674 | 0.0 | 650 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0078190 | A0A072VN49 | 94.353 | 726 | 22 | 1 | 1 | 726 | 1 | 707 | 0.0 | 1414 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0030780 | Msa0078190 | 0.981308 | 2.826697e-152 | -8.615850e-47 |
| Msa0078190 | Msa0170750 | 0.956456 | 2.829869e-114 | -8.615850e-47 |
| Msa0078190 | Msa0218370 | 0.822924 | 1.825660e-53 | -8.615850e-47 |
| Msa0078190 | Msa0545050 | 0.803726 | 3.037300e-49 | -8.615850e-47 |
| Msa0078190 | Msa0624060 | 0.802816 | 4.688907e-49 | -8.615850e-47 |
| Msa0078190 | Msa0676130 | 0.803435 | 3.490483e-49 | -8.615850e-47 |
| Msa0078190 | Msa0676180 | 0.807133 | 5.865576e-50 | -8.615850e-47 |
| Msa0078190 | Msa1008450 | 0.802429 | 5.635308e-49 | -8.615850e-47 |
| Msa0078190 | Msa1101220 | 0.801653 | 8.134132e-49 | -8.615850e-47 |
| Msa0078190 | Msa1122150 | 0.832831 | 7.551943e-56 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0078190 | MtrunA17_Chr1g0188421 | 93.322 | 584 | 20 | 1 | 143 | 726 | 1 | 565 | 0.0 | 1121 |
| Msa0078190 | MtrunA17_Chr4g0017901 | 48.189 | 718 | 331 | 11 | 2 | 706 | 1 | 690 | 0.0 | 663 |
| Msa0078190 | MtrunA17_Chr5g0434341 | 29.920 | 752 | 372 | 20 | 13 | 691 | 35 | 704 | 4.35e-89 | 295 |
| Msa0078190 | MtrunA17_Chr1g0188411 | 99.115 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 7.60e-74 | 234 |
| Msa0078190 | MtrunA17_Chr5g0417171 | 27.790 | 457 | 294 | 13 | 259 | 688 | 1 | 448 | 4.81e-50 | 181 |
| Msa0078190 | MtrunA17_Chr5g0417181 | 57.407 | 54 | 22 | 1 | 195 | 248 | 11 | 63 | 8.71e-14 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0078190 | AT1G33420.1 | 48.338 | 722 | 312 | 19 | 2 | 712 | 3 | 674 | 0.0 | 650 |
| Msa0078190 | AT1G33420.2 | 48.600 | 607 | 256 | 15 | 113 | 712 | 12 | 569 | 0.0 | 551 |
| Msa0078190 | AT5G22260.1 | 28.931 | 636 | 376 | 16 | 86 | 693 | 78 | 665 | 1.33e-82 | 276 |
| Msa0078190 | AT1G66170.1 | 31.547 | 653 | 330 | 19 | 83 | 686 | 69 | 653 | 2.37e-80 | 271 |
| Msa0078190 | AT2G01810.1 | 27.019 | 718 | 380 | 23 | 39 | 686 | 39 | 682 | 5.32e-69 | 240 |
| Msa0078190 | AT2G07714.1 | 34.746 | 118 | 74 | 1 | 233 | 347 | 1 | 118 | 1.59e-20 | 90.5 |
| Msa0078190 | ATMG00550.1 | 31.522 | 92 | 60 | 1 | 259 | 347 | 1 | 92 | 9.30e-12 | 64.3 |
Find 249 sgRNAs with CRISPR-Local
Find 461 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTGGAGTGTATGAATTC+AGG | 0.437264 | 1_2:+55563798 | Msa0078190:CDS |
| AAAGAAATCGTGGTAATCGG+CGG | 0.688894 | 1_2:-55560159 | None:intergenic |
| AAAGAGTGACGTTGAACGTG+TGG | 0.580901 | 1_2:+55563601 | Msa0078190:CDS |
| AAAGCTGATGGGAGATCCAT+TGG | 0.526083 | 1_2:+55561689 | Msa0078190:CDS |
| AAATAACAGATAGGCATTGG+TGG | 0.591327 | 1_2:+55565574 | Msa0078190:three_prime_UTR |
| AAATCCATGGTATGGTGAGT+GGG | 0.494538 | 1_2:+55563277 | Msa0078190:CDS |
| AAGAACTCTTGCAATGAGCT+CGG | 0.581985 | 1_2:+55564831 | Msa0078190:CDS |
| AAGAAGGTGCCCATAACCAT+TGG | 0.553682 | 1_2:-55562675 | None:intergenic |
| AAGATGGTTATTGAGTTTGA+TGG | 0.432591 | 1_2:-55559996 | None:intergenic |
| AAGCCATGCTCAGAGTGCTG+CGG | 0.591353 | 1_2:+55563627 | Msa0078190:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTCTTCTTGTATGTTATGTG+GGG | - | chr1_2:55559878-55559897 | None:intergenic | 30.0% | |
| AAGATGGTTATTGAGTTTGA+TGG | - | chr1_2:55559999-55560018 | None:intergenic | 30.0% | |
| TGAAAAGAAATCGTGGTAAT+CGG | - | chr1_2:55560165-55560184 | None:intergenic | 30.0% | |
| CGATAAAATGGAGAGATATT+AGG | + | chr1_2:55560816-55560835 | Msa0078190:intron | 30.0% | |
| TAGGGAGAAGAGAATATAAA+TGG | + | chr1_2:55560835-55560854 | Msa0078190:intron | 30.0% | |
| GAATATAAATGGTATTTGGG+TGG | + | chr1_2:55560846-55560865 | Msa0078190:intron | 30.0% | |
| TTGTGCTAAAAAGTTATGCT+TGG | + | chr1_2:55561009-55561028 | Msa0078190:intron | 30.0% | |
| ACAACTAAGCAAAATCCAAA+TGG | - | chr1_2:55561095-55561114 | None:intergenic | 30.0% | |
| AATGGAGAAAAATACTCACT+TGG | - | chr1_2:55561768-55561787 | None:intergenic | 30.0% | |
| AGGATGACAATTGCATAAAA+TGG | - | chr1_2:55561786-55561805 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 55559755 | 55565681 | 55559755 | ID=Msa0078190;Name=Msa0078190 |
| chr1_2 | mRNA | 55559755 | 55565681 | 55559755 | ID=Msa0078190-mRNA-1;Parent=Msa0078190;Name=Msa0078190-mRNA-1;_AED=0.10;_eAED=0.10;_QI=355|0.6|0.83|0.83|1|1|6|169|726 |
| chr1_2 | exon | 55559755 | 55560446 | 55559755 | ID=Msa0078190-mRNA-1:exon:17310;Parent=Msa0078190-mRNA-1 |
| chr1_2 | exon | 55561642 | 55561768 | 55561642 | ID=Msa0078190-mRNA-1:exon:17311;Parent=Msa0078190-mRNA-1 |
| chr1_2 | exon | 55561842 | 55561898 | 55561842 | ID=Msa0078190-mRNA-1:exon:17312;Parent=Msa0078190-mRNA-1 |
| chr1_2 | exon | 55562574 | 55562789 | 55562574 | ID=Msa0078190-mRNA-1:exon:17313;Parent=Msa0078190-mRNA-1 |
| chr1_2 | exon | 55563205 | 55563819 | 55563205 | ID=Msa0078190-mRNA-1:exon:17314;Parent=Msa0078190-mRNA-1 |
| chr1_2 | exon | 55564684 | 55565681 | 55564684 | ID=Msa0078190-mRNA-1:exon:17315;Parent=Msa0078190-mRNA-1 |
| chr1_2 | five_prime_UTR | 55559755 | 55560109 | 55559755 | ID=Msa0078190-mRNA-1:five_prime_utr;Parent=Msa0078190-mRNA-1 |
| chr1_2 | CDS | 55560110 | 55560446 | 55560110 | ID=Msa0078190-mRNA-1:cds;Parent=Msa0078190-mRNA-1 |
| chr1_2 | CDS | 55561642 | 55561768 | 55561642 | ID=Msa0078190-mRNA-1:cds;Parent=Msa0078190-mRNA-1 |
| chr1_2 | CDS | 55561842 | 55561898 | 55561842 | ID=Msa0078190-mRNA-1:cds;Parent=Msa0078190-mRNA-1 |
| chr1_2 | CDS | 55562574 | 55562789 | 55562574 | ID=Msa0078190-mRNA-1:cds;Parent=Msa0078190-mRNA-1 |
| chr1_2 | CDS | 55563205 | 55563819 | 55563205 | ID=Msa0078190-mRNA-1:cds;Parent=Msa0078190-mRNA-1 |
| chr1_2 | CDS | 55564684 | 55565512 | 55564684 | ID=Msa0078190-mRNA-1:cds;Parent=Msa0078190-mRNA-1 |
| chr1_2 | three_prime_UTR | 55565513 | 55565681 | 55565513 | ID=Msa0078190-mRNA-1:three_prime_utr;Parent=Msa0078190-mRNA-1 |
| Gene Sequence |
>Msa0078190 ATGGTCGTTAGCGGAGGCAAATCCTTAAAACGTATGAAAAGAAGAGTCACCGCCGATTACCACGATTTCTTTTCATTCCCTTCACCATCCCTCGCCGCTTCAGAGAGTTTCTCCGGCGGACCTTTTCGTTCTAATGTTCACTCTTTTCTCACCAAGTACGCGCTTCTTCCACCGCCTTCCGCGTTGTTTCCGCACTTGCTGACGTGGCAGATCCTCTTCCGTGTCGGTGAGATCACCGATGGGGGTGAATCGGGACCGGCGGTTATTTGCCTTGACGTTGTGGAAGAAGATGTTGCTAGATCCAGATCTGTGTATTGTGATCAGTGCCGAGTTTTTGGCTGCAGCGGAAATCCAGTGTGCAGTAAGCGTTACCATTTTATAATCAAAGCTGATGGGAGATCCATTGGTGGATACCACAAACCATGTATGTGCTGTGGAGATATTTTGCATTTGTCAGAATCCAAGCTTCCCTCAATGGGGGAAGATTATACCCAACCATCCCTCAATGGGGATGGAATCTTGTGCAAGTCATGTAACCATGTGACAACCAGTGATGATGTTGAAGACTGGGTGTACCACCAACTAGAGAACACAAGTCATCTTTTACATGGTGTAGTCCATGCCAATGGTTATGGGCACCTTCTTCGAGTTAATGGCAGAGAAGGGGGATCTAGATATCTTTCTGGTTGCCATATTATGGATTTCTGGGATCGCCTATGCAAGACACTTGGAGCTAGAAAAGTTAGTGTGATGGATGTTTCGAAGAAATACGGGCTAGAGTATCGCTTGCTCCATGCCATTATGAAGGGAAATCCATGGTATGGTGAGTGGGGATATAATTTTGGCTCTGGCAGCTATTGTCTCACACAGGAAGCATACAAGTCTGCTGTTGAAAGCCTATCCAATCTACCCTTGTCCATCTTCCTCTCCCAGGAACAGAAGCCTCACTCACGTGTGCAGGATATGATTTTGTATTACCGGTCTTTGTCAGAGCATGAGCTTGTAAATATGAGGGACCTATTTTGTTTTTTGATGGGTTTGTTGCATGATGCTCGCAAGACAGCCTCTAAGTCTGATGACATAACCTGCAAAAAGCGGCGGATTGATGCTTCTGGATTATCAAGTTCTTGGGAAAAGAGTGACGTTGAACGTGTGGAGGAAGCCATGCTCAGAGTGCTGCGGGCAGTGTCTGGGTCTAATTGGGTGAGTGGTCGAGCTCTTAAGGGTGCTGTTTGCAAGGTGGCCTCTCCAGAGCTTCTTGATTATTGCCTTTCTGAACTGGGAGGAAAAGTAGTCTATGGTGGGATTGTTAATAGCAGATGCAATCCTCAAACTGGAGTGTATGAATTCAGGATTGATGCTACAAATGTTTTTATGGCCAACAATAATTCTCCAGGCTCAAAGCGTCCTTCTGAAGAGAATATCCTGCAATGCTTGAGATATTTATACGACTCTCTGCTTCATCCTTGGATGATGCTAAACTATGTTGATGAAGAGACAAGAACTCTTGCAATGAGCTCGGCTCAGAAGCTTCTTGACTGCAAGCAATTAGTAAAAGATTATTGCTCTGAGATGCCGCCAGTGTCAGATTTGTCTAAATTACGTATCTCATGTCAGGTTGAACTAGTTGACCAATCTGAAGATACTGAAGCCAAAATTCCACCAGAAATAATTGTGCTGCCCATGAACGCCACAGTGTCTGACCTAAAGATTGAAGCAGCAAAAGCTTTTCAGGAAGTATATCTAATGTTTAGAAAATTTCAAGTTGACGAACTAGTTGGCTACAGCGGTGTAGAAGATTCCACCCAGGTCAAGTTCTTGTTAGGATCAAATGAAGTGGTTTGTGTCCGAGGAAAATACTCCGGGAAGAATGGGTTGAGCAAGTTTCGAATGGAACGTGGACTTGAGAGGTGGACTGTCGAGTGCAACTGTGGGGCTAAGGATGATGATGGAGAGAGAATGATGGCTTGTGATATGTGTGGTGTGTGGCGGCACACTAGGTGTTTTGGCATTCCTGATACTGCTCCTGTTCCAGCCCGTTTTGTTTGTTCGAGATGCCAAAACTGTGACTCAAAGCTTAAATCGGGTGGCCACTGTAAAGATGAGAGACTGGCTAGTGTTACCACAACTAGTGGTAGTTACATGGGAAACAGCTTTTCAGTGCCTTCTAATATTCGTTAA |
| Protein sequence |
>Msa0078190 MVVSGGKSLKRMKRRVTADYHDFFSFPSPSLAASESFSGGPFRSNVHSFLTKYALLPPPSALFPHLLTWQILFRVGEITDGGESGPAVICLDVVEEDVARSRSVYCDQCRVFGCSGNPVCSKRYHFIIKADGRSIGGYHKPCMCCGDILHLSESKLPSMGEDYTQPSLNGDGILCKSCNHVTTSDDVEDWVYHQLENTSHLLHGVVHANGYGHLLRVNGREGGSRYLSGCHIMDFWDRLCKTLGARKVSVMDVSKKYGLEYRLLHAIMKGNPWYGEWGYNFGSGSYCLTQEAYKSAVESLSNLPLSIFLSQEQKPHSRVQDMILYYRSLSEHELVNMRDLFCFLMGLLHDARKTASKSDDITCKKRRIDASGLSSSWEKSDVERVEEAMLRVLRAVSGSNWVSGRALKGAVCKVASPELLDYCLSELGGKVVYGGIVNSRCNPQTGVYEFRIDATNVFMANNNSPGSKRPSEENILQCLRYLYDSLLHPWMMLNYVDEETRTLAMSSAQKLLDCKQLVKDYCSEMPPVSDLSKLRISCQVELVDQSEDTEAKIPPEIIVLPMNATVSDLKIEAAKAFQEVYLMFRKFQVDELVGYSGVEDSTQVKFLLGSNEVVCVRGKYSGKNGLSKFRMERGLERWTVECNCGAKDDDGERMMACDMCGVWRHTRCFGIPDTAPVPARFVCSRCQNCDSKLKSGGHCKDERLASVTTTSGSYMGNSFSVPSNIR* |
