AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0120520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0120520 MtrunA17_Chr1g0179001 98.214 112 2 0 1 112 1 112 3.05e-71 207
Msa0120520 MtrunA17_Chr2g0321121 73.451 113 29 1 1 112 1 113 1.97e-37 122
Msa0120520 MtrunA17_Chr5g0421491 68.142 113 35 1 1 112 1 113 3.96e-29 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0120520 AT3G44590.1 72.321 112 30 1 1 112 1 111 2.13e-33 112
Msa0120520 AT3G44590.2 72.321 112 30 1 1 112 1 111 2.13e-33 112
Msa0120520 AT2G27710.4 72.857 70 19 0 1 70 1 70 2.28e-29 102
Msa0120520 AT2G27720.1 67.241 116 33 2 1 112 1 115 2.18e-25 92.4
Msa0120520 AT2G27710.1 71.212 66 19 0 1 66 1 66 1.20e-22 85.5
Msa0120520 AT2G27710.2 71.212 66 19 0 1 66 1 66 1.20e-22 85.5
Msa0120520 AT2G27710.3 71.212 66 19 0 1 66 1 66 1.20e-22 85.5
Msa0120520 AT2G27720.3 60.938 128 33 3 1 112 1 127 8.30e-22 83.6
Msa0120520 AT2G27720.2 58.779 131 34 3 1 112 1 130 2.09e-20 80.1
Msa0120520 AT3G28500.1 47.863 117 54 4 1 112 1 115 2.49e-20 79.7
Msa0120520 AT5G40040.1 53.846 65 30 0 1 65 1 65 7.21e-17 70.9
Msa0120520 AT2G27720.4 68.182 88 23 2 29 112 5 91 2.60e-11 55.8

Find 41 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTATTTGCTCGCTGTTCT+TGG 0.258518 1_3:+45457915 Msa0120520:CDS
GAGGATGTCCTTGATGGTTT+TGG 0.299226 1_3:-45457958 None:intergenic
ATAGCTGAGGTTATTGCTTC+TGG 0.312841 1_3:+45458546 Msa0120520:CDS
TTCTTTGCCTCAGCAGCTGC+AGG 0.352045 1_3:-45458648 None:intergenic
TTAATGTTGGAGTTATATCT+TGG 0.399833 1_3:+45459647 Msa0120520:three_prime_UTR
TGCTGCTCCTGCAGCTGCTG+AGG 0.400029 1_3:+45458641 Msa0120520:CDS
AAATTGGCATCAGTTCCTTC+TGG 0.406339 1_3:+45458576 Msa0120520:CDS
GCTAAAGCAATAGAAATTGA+TGG 0.409254 1_3:+45459731 Msa0120520:three_prime_UTR
TTTCTTGCAATGCAGGATAT+GGG 0.416445 1_3:+45459495 Msa0120520:intron
TCAATATATTCGAGGGCAAC+TGG 0.416883 1_3:-45459809 None:intergenic
GGATCCGAGGATGTCCTTGA+TGG 0.416992 1_3:-45457964 None:intergenic
TAAGTTGGATGGATTCAGTT+TGG 0.420711 1_3:+45459612 Msa0120520:three_prime_UTR
GTTCAGAGATTTCAATTTCA+AGG 0.425896 1_3:-45457834 None:intergenic
CGCCGTCAGCGTAAAGATGA+AGG 0.464823 1_3:+45457884 Msa0120520:exon
ACCAAGAACAGCGAGCAAAT+AGG 0.468861 1_3:-45457916 None:intergenic
CAAATAAGAAACGAAATGTC+TGG 0.470083 1_3:-45459704 None:intergenic
ATCAAGGACATCCTCGGATC+CGG 0.474041 1_3:+45457966 Msa0120520:CDS
GAGCAGACGCAGAAGATGAT+AGG 0.489365 1_3:+45458487 Msa0120520:CDS
GCATCAGTTCCTTCTGGTGG+TGG 0.495446 1_3:+45458582 Msa0120520:CDS
TTCAGAGATTTCAATTTCAA+GGG 0.498429 1_3:-45457833 None:intergenic
TCAGTTCCTTCTGGTGGTGG+CGG 0.512709 1_3:+45458585 Msa0120520:CDS
AACCTTCATCTTTACGCTGA+CGG 0.523625 1_3:-45457886 None:intergenic
GCTGCTGCACCTGCGAGTGG+TGG 0.523717 1_3:+45458618 Msa0120520:CDS
GTTGCTGCTGCACCTGCGAG+TGG 0.531011 1_3:+45458615 Msa0120520:CDS
AGCTGCTGAGGCAAAGAAAG+AGG 0.531573 1_3:+45458653 Msa0120520:CDS
TGAGGTTATTGCTTCTGGTA+GGG 0.533768 1_3:+45458551 Msa0120520:CDS
TATTTGCTCGCTGTTCTTGG+TGG 0.547005 1_3:+45457918 Msa0120520:CDS
CTGAGGTTATTGCTTCTGGT+AGG 0.548098 1_3:+45458550 Msa0120520:CDS
GGAGCAGCACCACCACTCGC+AGG 0.548277 1_3:-45458627 None:intergenic
CCCCTCCGCCAAAACCATCA+AGG 0.565900 1_3:+45457950 Msa0120520:CDS
GGCAAAGAAAGAGGAGAAGG+TGG 0.566691 1_3:+45458662 Msa0120520:CDS
TGAGGCAAAGAAAGAGGAGA+AGG 0.574103 1_3:+45458659 Msa0120520:CDS
GAAGAAACGAACCGGATCCG+AGG 0.577935 1_3:-45457977 None:intergenic
AAAACCATCAAGGACATCCT+CGG 0.583067 1_3:+45457960 Msa0120520:CDS
GCTGTGTATCAATATATTCG+AGG 0.592134 1_3:-45459817 None:intergenic
CTGTGTATCAATATATTCGA+GGG 0.593876 1_3:-45459816 None:intergenic
TTGGCATCAGTTCCTTCTGG+TGG 0.606228 1_3:+45458579 Msa0120520:CDS
GTTTGAGAATGAAATGCAAG+AGG 0.633797 1_3:-45457862 None:intergenic
TTTGAGAATGAAATGCAAGA+GGG 0.657666 1_3:-45457861 None:intergenic
GCAACACCGCCACCACCAGA+AGG 0.663456 1_3:-45458591 None:intergenic
TAAGGGCAAAGATATAGCTG+AGG 0.689474 1_3:+45458533 Msa0120520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGATTTCGATTTTTTTAT+CGG + chr1_3:45458166-45458185 Msa0120520:intron 15.0%
!!! ATTTTTTAGAACTGTTTTTA+AGG + chr1_3:45458228-45458247 Msa0120520:intron 15.0%
!!! AAGTTTTGTTTGATATATAA+TGG + chr1_3:45459560-45459579 Msa0120520:three_prime_UTR 15.0%
!!! TTTGATTTCGATTTTTTTAT+CGG + chr1_3:45458166-45458185 Msa0120520:intron 15.0%
!!! ATTTTTTAGAACTGTTTTTA+AGG + chr1_3:45458228-45458247 Msa0120520:intron 15.0%
!!! AAGTTTTGTTTGATATATAA+TGG + chr1_3:45459560-45459579 Msa0120520:three_prime_UTR 15.0%
!!! TTTTCGATGGATTTAATTAT+GGG - chr1_3:45457779-45457798 None:intergenic 20.0%
!!! TTTTTCGATGGATTTAATTA+TGG - chr1_3:45457780-45457799 None:intergenic 20.0%
!!! TTTGTGTAAAATGGATTTTA+GGG - chr1_3:45457812-45457831 None:intergenic 20.0%
!!! TTTTGTGTAAAATGGATTTT+AGG - chr1_3:45457813-45457832 None:intergenic 20.0%
!!! TTGTTTTTGTCTGAAATTAA+GGG + chr1_3:45458516-45458535 Msa0120520:CDS 20.0%
!! AAATGACTAAAAAGATTGAA+TGG - chr1_3:45459079-45459098 None:intergenic 20.0%
!! TATACAACTGTAATGTTATA+TGG + chr1_3:45459295-45459314 Msa0120520:intron 20.0%
!! ATACAACTGTAATGTTATAT+GGG + chr1_3:45459296-45459315 Msa0120520:intron 20.0%
!! TCAATGATTAAAAACTAAGT+TGG + chr1_3:45459597-45459616 Msa0120520:three_prime_UTR 20.0%
!!! GTATTTTAGTTTCTTAATGT+TGG + chr1_3:45459634-45459653 Msa0120520:three_prime_UTR 20.0%
!!! AGATGCAAATTTTAATTAGA+TGG - chr1_3:45459759-45459778 None:intergenic 20.0%
!!! TTTTCGATGGATTTAATTAT+GGG - chr1_3:45457779-45457798 None:intergenic 20.0%
!!! TTTTTCGATGGATTTAATTA+TGG - chr1_3:45457780-45457799 None:intergenic 20.0%
!!! TTTGTGTAAAATGGATTTTA+GGG - chr1_3:45457812-45457831 None:intergenic 20.0%
!!! TTTTGTGTAAAATGGATTTT+AGG - chr1_3:45457813-45457832 None:intergenic 20.0%
!!! TTGTTTTTGTCTGAAATTAA+GGG + chr1_3:45458516-45458535 Msa0120520:CDS 20.0%
!! AAATGACTAAAAAGATTGAA+TGG - chr1_3:45459079-45459098 None:intergenic 20.0%
!! TATACAACTGTAATGTTATA+TGG + chr1_3:45459295-45459314 Msa0120520:intron 20.0%
!! ATACAACTGTAATGTTATAT+GGG + chr1_3:45459296-45459315 Msa0120520:intron 20.0%
!! TCAATGATTAAAAACTAAGT+TGG + chr1_3:45459597-45459616 Msa0120520:three_prime_UTR 20.0%
!!! GTATTTTAGTTTCTTAATGT+TGG + chr1_3:45459634-45459653 Msa0120520:three_prime_UTR 20.0%
!!! AGATGCAAATTTTAATTAGA+TGG - chr1_3:45459759-45459778 None:intergenic 20.0%
! TTCAGAGATTTCAATTTCAA+GGG - chr1_3:45457836-45457855 None:intergenic 25.0%
!!! GTTGTTTTTGTCTGAAATTA+AGG + chr1_3:45458515-45458534 Msa0120520:CDS 25.0%
!! ATGATGTAAGTTCTTTTTCT+GGG + chr1_3:45458703-45458722 Msa0120520:intron 25.0%
! CACATAATGCAAATTAAACT+AGG - chr1_3:45458821-45458840 None:intergenic 25.0%
!! TATGTGTTTTATACTCGAAA+AGG + chr1_3:45458835-45458854 Msa0120520:intron 25.0%
! TTCAGCATATATATCAAAAG+CGG - chr1_3:45459006-45459025 None:intergenic 25.0%
!! CTTTTATGTGATTATGTGTA+TGG + chr1_3:45459206-45459225 Msa0120520:intron 25.0%
! TCATAGAATCACTCATAATA+TGG + chr1_3:45459352-45459371 Msa0120520:intron 25.0%
! AGTAGTTATGTATATTGTCT+TGG + chr1_3:45459412-45459431 Msa0120520:intron 25.0%
!! TGATTAAAAACTAAGTTGGA+TGG + chr1_3:45459601-45459620 Msa0120520:three_prime_UTR 25.0%
! TTAATGTTGGAGTTATATCT+TGG + chr1_3:45459647-45459666 Msa0120520:three_prime_UTR 25.0%
! TTCAGAGATTTCAATTTCAA+GGG - chr1_3:45457836-45457855 None:intergenic 25.0%
!!! GTTGTTTTTGTCTGAAATTA+AGG + chr1_3:45458515-45458534 Msa0120520:CDS 25.0%
!! ATGATGTAAGTTCTTTTTCT+GGG + chr1_3:45458703-45458722 Msa0120520:intron 25.0%
! CACATAATGCAAATTAAACT+AGG - chr1_3:45458821-45458840 None:intergenic 25.0%
!! TATGTGTTTTATACTCGAAA+AGG + chr1_3:45458835-45458854 Msa0120520:intron 25.0%
! TTCAGCATATATATCAAAAG+CGG - chr1_3:45459006-45459025 None:intergenic 25.0%
!! CTTTTATGTGATTATGTGTA+TGG + chr1_3:45459206-45459225 Msa0120520:intron 25.0%
! TCATAGAATCACTCATAATA+TGG + chr1_3:45459352-45459371 Msa0120520:intron 25.0%
! AGTAGTTATGTATATTGTCT+TGG + chr1_3:45459412-45459431 Msa0120520:intron 25.0%
!! TGATTAAAAACTAAGTTGGA+TGG + chr1_3:45459601-45459620 Msa0120520:three_prime_UTR 25.0%
! TTAATGTTGGAGTTATATCT+TGG + chr1_3:45459647-45459666 Msa0120520:three_prime_UTR 25.0%
!! GGGTTTCATATATTTTTCGA+TGG - chr1_3:45457792-45457811 None:intergenic 30.0%
!! TTCAAGGGTTTTGTGTAAAA+TGG - chr1_3:45457821-45457840 None:intergenic 30.0%
GTTCAGAGATTTCAATTTCA+AGG - chr1_3:45457837-45457856 None:intergenic 30.0%
TTTGAGAATGAAATGCAAGA+GGG - chr1_3:45457864-45457883 None:intergenic 30.0%
TTTATGCAGTAGCGTAAATT+AGG + chr1_3:45458139-45458158 Msa0120520:intron 30.0%
TTATGCAGTAGCGTAAATTA+GGG + chr1_3:45458140-45458159 Msa0120520:intron 30.0%
ATCACATGTGTTAGTTGTAT+CGG + chr1_3:45458307-45458326 Msa0120520:intron 30.0%
! ACACATTCAATTAGAAGTGT+CGG + chr1_3:45458362-45458381 Msa0120520:intron 30.0%
! TGATTTGAAACATAACTGAC+AGG + chr1_3:45458439-45458458 Msa0120520:intron 30.0%
! GATTTGAAACATAACTGACA+GGG + chr1_3:45458440-45458459 Msa0120520:intron 30.0%
!! TTTGTTGTTTGATTTGCAGT+TGG + chr1_3:45458465-45458484 Msa0120520:intron 30.0%
! GATGATGTAAGTTCTTTTTC+TGG + chr1_3:45458702-45458721 Msa0120520:intron 30.0%
TTATATGTTTGCGCTATAAG+TGG + chr1_3:45458769-45458788 Msa0120520:intron 30.0%
!!! AGTGGTTTTAAACTTGCATT+TGG + chr1_3:45458787-45458806 Msa0120520:intron 30.0%
!! GTTTTAAACTTGCATTTGGT+TGG + chr1_3:45458791-45458810 Msa0120520:intron 30.0%
!! AAGGTTCTTGATTTGTACTT+AGG + chr1_3:45458854-45458873 Msa0120520:intron 30.0%
TGAACTGTGATTAATAGCTA+TGG + chr1_3:45459146-45459165 Msa0120520:intron 30.0%
AACATTACAGTTGTATAGCA+AGG - chr1_3:45459292-45459311 None:intergenic 30.0%
AACTGTAATGTTATATGGGT+TGG + chr1_3:45459300-45459319 Msa0120520:intron 30.0%
CAAATAAGAAACGAAATGTC+TGG - chr1_3:45459707-45459726 None:intergenic 30.0%
GCTAAAGCAATAGAAATTGA+TGG + chr1_3:45459731-45459750 Msa0120520:three_prime_UTR 30.0%
CTGTGTATCAATATATTCGA+GGG - chr1_3:45459819-45459838 None:intergenic 30.0%
!! GGGTTTCATATATTTTTCGA+TGG - chr1_3:45457792-45457811 None:intergenic 30.0%
!! TTCAAGGGTTTTGTGTAAAA+TGG - chr1_3:45457821-45457840 None:intergenic 30.0%
GTTCAGAGATTTCAATTTCA+AGG - chr1_3:45457837-45457856 None:intergenic 30.0%
TTTGAGAATGAAATGCAAGA+GGG - chr1_3:45457864-45457883 None:intergenic 30.0%
TTTATGCAGTAGCGTAAATT+AGG + chr1_3:45458139-45458158 Msa0120520:intron 30.0%
TTATGCAGTAGCGTAAATTA+GGG + chr1_3:45458140-45458159 Msa0120520:intron 30.0%
ATCACATGTGTTAGTTGTAT+CGG + chr1_3:45458307-45458326 Msa0120520:intron 30.0%
! ACACATTCAATTAGAAGTGT+CGG + chr1_3:45458362-45458381 Msa0120520:intron 30.0%
! TGATTTGAAACATAACTGAC+AGG + chr1_3:45458439-45458458 Msa0120520:intron 30.0%
! GATTTGAAACATAACTGACA+GGG + chr1_3:45458440-45458459 Msa0120520:intron 30.0%
!! TTTGTTGTTTGATTTGCAGT+TGG + chr1_3:45458465-45458484 Msa0120520:intron 30.0%
! GATGATGTAAGTTCTTTTTC+TGG + chr1_3:45458702-45458721 Msa0120520:intron 30.0%
TTATATGTTTGCGCTATAAG+TGG + chr1_3:45458769-45458788 Msa0120520:intron 30.0%
!!! AGTGGTTTTAAACTTGCATT+TGG + chr1_3:45458787-45458806 Msa0120520:intron 30.0%
!! GTTTTAAACTTGCATTTGGT+TGG + chr1_3:45458791-45458810 Msa0120520:intron 30.0%
!! AAGGTTCTTGATTTGTACTT+AGG + chr1_3:45458854-45458873 Msa0120520:intron 30.0%
TGAACTGTGATTAATAGCTA+TGG + chr1_3:45459146-45459165 Msa0120520:intron 30.0%
AACATTACAGTTGTATAGCA+AGG - chr1_3:45459292-45459311 None:intergenic 30.0%
AACTGTAATGTTATATGGGT+TGG + chr1_3:45459300-45459319 Msa0120520:intron 30.0%
CAAATAAGAAACGAAATGTC+TGG - chr1_3:45459707-45459726 None:intergenic 30.0%
GCTAAAGCAATAGAAATTGA+TGG + chr1_3:45459731-45459750 Msa0120520:three_prime_UTR 30.0%
CTGTGTATCAATATATTCGA+GGG - chr1_3:45459819-45459838 None:intergenic 30.0%
GTTTGAGAATGAAATGCAAG+AGG - chr1_3:45457865-45457884 None:intergenic 35.0%
AAGCAATTGAAGAAACGAAC+CGG - chr1_3:45457988-45458007 None:intergenic 35.0%
CTTCTAATTGAATGTGTGTC+CGG - chr1_3:45458359-45458378 None:intergenic 35.0%
AGCAGTGTTGCATAATTTAG+CGG + chr1_3:45458918-45458937 Msa0120520:intron 35.0%
ACTAGCCATTATGAAAAACC+CGG + chr1_3:45458943-45458962 Msa0120520:intron 35.0%
TACTAAAACCGTAATGCAAC+CGG - chr1_3:45458965-45458984 None:intergenic 35.0%
! ACTTCAAAAGTACAAGTTGG+GGG - chr1_3:45459052-45459071 None:intergenic 35.0%
! CACTTCAAAAGTACAAGTTG+GGG - chr1_3:45459053-45459072 None:intergenic 35.0%
! CCACTTCAAAAGTACAAGTT+GGG - chr1_3:45459054-45459073 None:intergenic 35.0%
! ACCACTTCAAAAGTACAAGT+TGG - chr1_3:45459055-45459074 None:intergenic 35.0%
TTTGCTAAACTTTGACGTAG+TGG + chr1_3:45459256-45459275 Msa0120520:intron 35.0%
TGATTCTATGAAGTCAATGG+CGG - chr1_3:45459343-45459362 None:intergenic 35.0%
GAGTGATTCTATGAAGTCAA+TGG - chr1_3:45459346-45459365 None:intergenic 35.0%
! TTTTCTTGCAATGCAGGATA+TGG + chr1_3:45459494-45459513 Msa0120520:intron 35.0%
TTTCTTGCAATGCAGGATAT+GGG + chr1_3:45459495-45459514 Msa0120520:intron 35.0%
! TAAGTTGGATGGATTCAGTT+TGG + chr1_3:45459612-45459631 Msa0120520:three_prime_UTR 35.0%
GCTGTGTATCAATATATTCG+AGG - chr1_3:45459820-45459839 None:intergenic 35.0%
GTTTGAGAATGAAATGCAAG+AGG - chr1_3:45457865-45457884 None:intergenic 35.0%
AAGCAATTGAAGAAACGAAC+CGG - chr1_3:45457988-45458007 None:intergenic 35.0%
CTTCTAATTGAATGTGTGTC+CGG - chr1_3:45458359-45458378 None:intergenic 35.0%
AGCAGTGTTGCATAATTTAG+CGG + chr1_3:45458918-45458937 Msa0120520:intron 35.0%
ACTAGCCATTATGAAAAACC+CGG + chr1_3:45458943-45458962 Msa0120520:intron 35.0%
TACTAAAACCGTAATGCAAC+CGG - chr1_3:45458965-45458984 None:intergenic 35.0%
! ACTTCAAAAGTACAAGTTGG+GGG - chr1_3:45459052-45459071 None:intergenic 35.0%
! CACTTCAAAAGTACAAGTTG+GGG - chr1_3:45459053-45459072 None:intergenic 35.0%
! CCACTTCAAAAGTACAAGTT+GGG - chr1_3:45459054-45459073 None:intergenic 35.0%
! ACCACTTCAAAAGTACAAGT+TGG - chr1_3:45459055-45459074 None:intergenic 35.0%
TTTGCTAAACTTTGACGTAG+TGG + chr1_3:45459256-45459275 Msa0120520:intron 35.0%
TGATTCTATGAAGTCAATGG+CGG - chr1_3:45459343-45459362 None:intergenic 35.0%
GAGTGATTCTATGAAGTCAA+TGG - chr1_3:45459346-45459365 None:intergenic 35.0%
! TTTTCTTGCAATGCAGGATA+TGG + chr1_3:45459494-45459513 Msa0120520:intron 35.0%
TTTCTTGCAATGCAGGATAT+GGG + chr1_3:45459495-45459514 Msa0120520:intron 35.0%
! TAAGTTGGATGGATTCAGTT+TGG + chr1_3:45459612-45459631 Msa0120520:three_prime_UTR 35.0%
GCTGTGTATCAATATATTCG+AGG - chr1_3:45459820-45459839 None:intergenic 35.0%
AACCTTCATCTTTACGCTGA+CGG - chr1_3:45457889-45457908 None:intergenic 40.0%
AAAACCATCAAGGACATCCT+CGG + chr1_3:45457960-45457979 Msa0120520:CDS 40.0%
TATTCGACATTGACCTGTGT+TGG + chr1_3:45458330-45458349 Msa0120520:intron 40.0%
! AAGGTGTCATGTCAAATCAG+TGG - chr1_3:45458402-45458421 None:intergenic 40.0%
TGATTTGACATGACACCTTG+AGG + chr1_3:45458403-45458422 Msa0120520:intron 40.0%
GATTTGACATGACACCTTGA+GGG + chr1_3:45458404-45458423 Msa0120520:intron 40.0%
ATTTGACATGACACCTTGAG+GGG + chr1_3:45458405-45458424 Msa0120520:intron 40.0%
TAAGGGCAAAGATATAGCTG+AGG + chr1_3:45458533-45458552 Msa0120520:CDS 40.0%
ATAGCTGAGGTTATTGCTTC+TGG + chr1_3:45458546-45458565 Msa0120520:CDS 40.0%
TGAGGTTATTGCTTCTGGTA+GGG + chr1_3:45458551-45458570 Msa0120520:CDS 40.0%
TGCTTCTGGTAGGGAAAAAT+TGG + chr1_3:45458560-45458579 Msa0120520:CDS 40.0%
AAATTGGCATCAGTTCCTTC+TGG + chr1_3:45458576-45458595 Msa0120520:CDS 40.0%
ATTATGCAACACTGCTACAG+CGG - chr1_3:45458914-45458933 None:intergenic 40.0%
! GGGTTTTTCATAATGGCTAG+TGG - chr1_3:45458944-45458963 None:intergenic 40.0%
! TGCAACCGGGTTTTTCATAA+TGG - chr1_3:45458951-45458970 None:intergenic 40.0%
TGAAAAACCCGGTTGCATTA+CGG + chr1_3:45458954-45458973 Msa0120520:intron 40.0%
ACTAAAACCGTAATGCAACC+GGG - chr1_3:45458964-45458983 None:intergenic 40.0%
AGCGGTGTAATTGCTATAGA+TGG - chr1_3:45458988-45459007 None:intergenic 40.0%
!! CCCAACTTGTACTTTTGAAG+TGG + chr1_3:45459051-45459070 Msa0120520:intron 40.0%
TACAGTTGTATAGCAAGGCA+TGG - chr1_3:45459287-45459306 None:intergenic 40.0%
!!! CGACTTTTTTCTTGCAATGC+AGG + chr1_3:45459488-45459507 Msa0120520:intron 40.0%
TCAATATATTCGAGGGCAAC+TGG - chr1_3:45459812-45459831 None:intergenic 40.0%
AACCTTCATCTTTACGCTGA+CGG - chr1_3:45457889-45457908 None:intergenic 40.0%
AAAACCATCAAGGACATCCT+CGG + chr1_3:45457960-45457979 Msa0120520:CDS 40.0%
TATTCGACATTGACCTGTGT+TGG + chr1_3:45458330-45458349 Msa0120520:intron 40.0%
! AAGGTGTCATGTCAAATCAG+TGG - chr1_3:45458402-45458421 None:intergenic 40.0%
TGATTTGACATGACACCTTG+AGG + chr1_3:45458403-45458422 Msa0120520:intron 40.0%
GATTTGACATGACACCTTGA+GGG + chr1_3:45458404-45458423 Msa0120520:intron 40.0%
ATTTGACATGACACCTTGAG+GGG + chr1_3:45458405-45458424 Msa0120520:intron 40.0%
TAAGGGCAAAGATATAGCTG+AGG + chr1_3:45458533-45458552 Msa0120520:CDS 40.0%
ATAGCTGAGGTTATTGCTTC+TGG + chr1_3:45458546-45458565 Msa0120520:CDS 40.0%
TGAGGTTATTGCTTCTGGTA+GGG + chr1_3:45458551-45458570 Msa0120520:CDS 40.0%
TGCTTCTGGTAGGGAAAAAT+TGG + chr1_3:45458560-45458579 Msa0120520:CDS 40.0%
AAATTGGCATCAGTTCCTTC+TGG + chr1_3:45458576-45458595 Msa0120520:CDS 40.0%
ATTATGCAACACTGCTACAG+CGG - chr1_3:45458914-45458933 None:intergenic 40.0%
! GGGTTTTTCATAATGGCTAG+TGG - chr1_3:45458944-45458963 None:intergenic 40.0%
! TGCAACCGGGTTTTTCATAA+TGG - chr1_3:45458951-45458970 None:intergenic 40.0%
TGAAAAACCCGGTTGCATTA+CGG + chr1_3:45458954-45458973 Msa0120520:intron 40.0%
ACTAAAACCGTAATGCAACC+GGG - chr1_3:45458964-45458983 None:intergenic 40.0%
AGCGGTGTAATTGCTATAGA+TGG - chr1_3:45458988-45459007 None:intergenic 40.0%
!! CCCAACTTGTACTTTTGAAG+TGG + chr1_3:45459051-45459070 Msa0120520:intron 40.0%
TACAGTTGTATAGCAAGGCA+TGG - chr1_3:45459287-45459306 None:intergenic 40.0%
!!! CGACTTTTTTCTTGCAATGC+AGG + chr1_3:45459488-45459507 Msa0120520:intron 40.0%
TCAATATATTCGAGGGCAAC+TGG - chr1_3:45459812-45459831 None:intergenic 40.0%
ACCAAGAACAGCGAGCAAAT+AGG - chr1_3:45457919-45457938 None:intergenic 45.0%
TATTTGCTCGCTGTTCTTGG+TGG + chr1_3:45457918-45457937 Msa0120520:CDS 45.0%
!!! GATGTCCTTGATGGTTTTGG+CGG - chr1_3:45457958-45457977 None:intergenic 45.0%
!! GAGGATGTCCTTGATGGTTT+TGG - chr1_3:45457961-45457980 None:intergenic 45.0%
CTGAGGTTATTGCTTCTGGT+AGG + chr1_3:45458550-45458569 Msa0120520:CDS 45.0%
TGAGGCAAAGAAAGAGGAGA+AGG + chr1_3:45458659-45458678 Msa0120520:CDS 45.0%
ACCAAGAACAGCGAGCAAAT+AGG - chr1_3:45457919-45457938 None:intergenic 45.0%
TATTTGCTCGCTGTTCTTGG+TGG + chr1_3:45457918-45457937 Msa0120520:CDS 45.0%
!!! GATGTCCTTGATGGTTTTGG+CGG - chr1_3:45457958-45457977 None:intergenic 45.0%
!! GAGGATGTCCTTGATGGTTT+TGG - chr1_3:45457961-45457980 None:intergenic 45.0%
CTGAGGTTATTGCTTCTGGT+AGG + chr1_3:45458550-45458569 Msa0120520:CDS 45.0%
TGAGGCAAAGAAAGAGGAGA+AGG + chr1_3:45458659-45458678 Msa0120520:CDS 45.0%
!!! ATTTTAATAATTTGATAAAT+CGG + chr1_3:45458101-45458120 Msa0120520:intron 5.0%
!!! AAAATTTATTTTCTTTTTAT+TGG + chr1_3:45459105-45459124 Msa0120520:intron 5.0%
!!! ATTTTAATAATTTGATAAAT+CGG + chr1_3:45458101-45458120 Msa0120520:intron 5.0%
!!! AAAATTTATTTTCTTTTTAT+TGG + chr1_3:45459105-45459124 Msa0120520:intron 5.0%
GCCTATTTGCTCGCTGTTCT+TGG + chr1_3:45457915-45457934 Msa0120520:CDS 50.0%
!!! TCCTTGATGGTTTTGGCGGA+GGG - chr1_3:45457954-45457973 None:intergenic 50.0%
ATCAAGGACATCCTCGGATC+CGG + chr1_3:45457966-45457985 Msa0120520:CDS 50.0%
CATTGACCTGTGTTGGACAC+CGG + chr1_3:45458337-45458356 Msa0120520:intron 50.0%
GAGCAGACGCAGAAGATGAT+AGG + chr1_3:45458487-45458506 Msa0120520:CDS 50.0%
TTGGCATCAGTTCCTTCTGG+TGG + chr1_3:45458579-45458598 Msa0120520:CDS 50.0%
AGCTGCTGAGGCAAAGAAAG+AGG + chr1_3:45458653-45458672 Msa0120520:CDS 50.0%
GGCAAAGAAAGAGGAGAAGG+TGG + chr1_3:45458662-45458681 Msa0120520:CDS 50.0%
!! CAATGGCGGATAGTGATGTG+TGG - chr1_3:45459329-45459348 None:intergenic 50.0%
GCCTATTTGCTCGCTGTTCT+TGG + chr1_3:45457915-45457934 Msa0120520:CDS 50.0%
!!! TCCTTGATGGTTTTGGCGGA+GGG - chr1_3:45457954-45457973 None:intergenic 50.0%
ATCAAGGACATCCTCGGATC+CGG + chr1_3:45457966-45457985 Msa0120520:CDS 50.0%
CATTGACCTGTGTTGGACAC+CGG + chr1_3:45458337-45458356 Msa0120520:intron 50.0%
GAGCAGACGCAGAAGATGAT+AGG + chr1_3:45458487-45458506 Msa0120520:CDS 50.0%
TTGGCATCAGTTCCTTCTGG+TGG + chr1_3:45458579-45458598 Msa0120520:CDS 50.0%
AGCTGCTGAGGCAAAGAAAG+AGG + chr1_3:45458653-45458672 Msa0120520:CDS 50.0%
GGCAAAGAAAGAGGAGAAGG+TGG + chr1_3:45458662-45458681 Msa0120520:CDS 50.0%
!! CAATGGCGGATAGTGATGTG+TGG - chr1_3:45459329-45459348 None:intergenic 50.0%
CGCCGTCAGCGTAAAGATGA+AGG + chr1_3:45457884-45457903 Msa0120520:exon 55.0%
!!! CTTGATGGTTTTGGCGGAGG+GGG - chr1_3:45457952-45457971 None:intergenic 55.0%
!!! CCTTGATGGTTTTGGCGGAG+GGG - chr1_3:45457953-45457972 None:intergenic 55.0%
!!! GTCCTTGATGGTTTTGGCGG+AGG - chr1_3:45457955-45457974 None:intergenic 55.0%
GGATCCGAGGATGTCCTTGA+TGG - chr1_3:45457967-45457986 None:intergenic 55.0%
GAAGAAACGAACCGGATCCG+AGG - chr1_3:45457980-45457999 None:intergenic 55.0%
CAGCGAGTTCAAACCCCTCA+AGG - chr1_3:45458421-45458440 None:intergenic 55.0%
GCATCAGTTCCTTCTGGTGG+TGG + chr1_3:45458582-45458601 Msa0120520:CDS 55.0%
!! TCAGTTCCTTCTGGTGGTGG+CGG + chr1_3:45458585-45458604 Msa0120520:CDS 55.0%
TTCTTTGCCTCAGCAGCTGC+AGG - chr1_3:45458651-45458670 None:intergenic 55.0%
AGCGGCTGCGATTGACTACA+GGG - chr1_3:45458896-45458915 None:intergenic 55.0%
CGCCGTCAGCGTAAAGATGA+AGG + chr1_3:45457884-45457903 Msa0120520:exon 55.0%
!!! CTTGATGGTTTTGGCGGAGG+GGG - chr1_3:45457952-45457971 None:intergenic 55.0%
!!! CCTTGATGGTTTTGGCGGAG+GGG - chr1_3:45457953-45457972 None:intergenic 55.0%
!!! GTCCTTGATGGTTTTGGCGG+AGG - chr1_3:45457955-45457974 None:intergenic 55.0%
GGATCCGAGGATGTCCTTGA+TGG - chr1_3:45457967-45457986 None:intergenic 55.0%
GAAGAAACGAACCGGATCCG+AGG - chr1_3:45457980-45457999 None:intergenic 55.0%
CAGCGAGTTCAAACCCCTCA+AGG - chr1_3:45458421-45458440 None:intergenic 55.0%
GCATCAGTTCCTTCTGGTGG+TGG + chr1_3:45458582-45458601 Msa0120520:CDS 55.0%
!! TCAGTTCCTTCTGGTGGTGG+CGG + chr1_3:45458585-45458604 Msa0120520:CDS 55.0%
TTCTTTGCCTCAGCAGCTGC+AGG - chr1_3:45458651-45458670 None:intergenic 55.0%
AGCGGCTGCGATTGACTACA+GGG - chr1_3:45458896-45458915 None:intergenic 55.0%
CCCCTCCGCCAAAACCATCA+AGG + chr1_3:45457950-45457969 Msa0120520:CDS 60.0%
GTGTGTCCGGTGTCCAACAC+AGG - chr1_3:45458346-45458365 None:intergenic 60.0%
GCGGCTGCGATTGACTACAG+GGG - chr1_3:45458895-45458914 None:intergenic 60.0%
CAGCGGCTGCGATTGACTAC+AGG - chr1_3:45458897-45458916 None:intergenic 60.0%
CCCCTCCGCCAAAACCATCA+AGG + chr1_3:45457950-45457969 Msa0120520:CDS 60.0%
GTGTGTCCGGTGTCCAACAC+AGG - chr1_3:45458346-45458365 None:intergenic 60.0%
GCGGCTGCGATTGACTACAG+GGG - chr1_3:45458895-45458914 None:intergenic 60.0%
CAGCGGCTGCGATTGACTAC+AGG - chr1_3:45458897-45458916 None:intergenic 60.0%
GCAACACCGCCACCACCAGA+AGG - chr1_3:45458594-45458613 None:intergenic 65.0%
GTTGCTGCTGCACCTGCGAG+TGG + chr1_3:45458615-45458634 Msa0120520:CDS 65.0%
TGCTGCTCCTGCAGCTGCTG+AGG + chr1_3:45458641-45458660 Msa0120520:CDS 65.0%
GCAACACCGCCACCACCAGA+AGG - chr1_3:45458594-45458613 None:intergenic 65.0%
GTTGCTGCTGCACCTGCGAG+TGG + chr1_3:45458615-45458634 Msa0120520:CDS 65.0%
TGCTGCTCCTGCAGCTGCTG+AGG + chr1_3:45458641-45458660 Msa0120520:CDS 65.0%
GCTGCTGCACCTGCGAGTGG+TGG + chr1_3:45458618-45458637 Msa0120520:CDS 70.0%
GGAGCAGCACCACCACTCGC+AGG - chr1_3:45458630-45458649 None:intergenic 70.0%
GCTGCTGCACCTGCGAGTGG+TGG + chr1_3:45458618-45458637 Msa0120520:CDS 70.0%
GGAGCAGCACCACCACTCGC+AGG - chr1_3:45458630-45458649 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 45457768 45459850 45457768 ID=Msa0120520;Name=Msa0120520
chr1_3 mRNA 45457768 45459850 45457768 ID=Msa0120520-mRNA-1;Parent=Msa0120520;Name=Msa0120520-mRNA-1;_AED=0.06;_eAED=0.06;_QI=132|1|1|1|1|1|3|314|112
chr1_3 exon 45457768 45457987 45457768 ID=Msa0120520-mRNA-1:exon:13594;Parent=Msa0120520-mRNA-1
chr1_3 exon 45458484 45458707 45458484 ID=Msa0120520-mRNA-1:exon:13595;Parent=Msa0120520-mRNA-1
chr1_3 exon 45459510 45459850 45459510 ID=Msa0120520-mRNA-1:exon:13596;Parent=Msa0120520-mRNA-1
chr1_3 five_prime_UTR 45457768 45457899 45457768 ID=Msa0120520-mRNA-1:five_prime_utr;Parent=Msa0120520-mRNA-1
chr1_3 CDS 45457900 45457987 45457900 ID=Msa0120520-mRNA-1:cds;Parent=Msa0120520-mRNA-1
chr1_3 CDS 45458484 45458707 45458484 ID=Msa0120520-mRNA-1:cds;Parent=Msa0120520-mRNA-1
chr1_3 CDS 45459510 45459536 45459510 ID=Msa0120520-mRNA-1:cds;Parent=Msa0120520-mRNA-1
chr1_3 three_prime_UTR 45459537 45459850 45459537 ID=Msa0120520-mRNA-1:three_prime_utr;Parent=Msa0120520-mRNA-1
Gene Sequence

>Msa0120520

ATGAAGGTTGTTGCTGCCTATTTGCTCGCTGTTCTTGGTGGCGACAACACCCCCTCCGCCAAAACCATCAAGGACATCCTCGGATCCGTTGGAGCAGACGCAGAAGATGATAGGATTGAGTTGTTTTTGTCTGAAATTAAGGGCAAAGATATAGCTGAGGTTATTGCTTCTGGTAGGGAAAAATTGGCATCAGTTCCTTCTGGTGGTGGCGGTGTTGCTGTTGCTGCTGCACCTGCGAGTGGTGGTGCTGCTCCTGCAGCTGCTGAGGCAAAGAAAGAGGAGAAGGTGGAAGAGAAAGAAGAATCTGATGATGATATGGGTTTCAGTCTTTTTGATTAA
Protein sequence

>Msa0120520

MKVVAAYLLAVLGGDNTPSAKTIKDILGSVGADAEDDRIELFLSEIKGKDIAEVIASGREKLASVPSGGGGVAVAAAPASGGAAPAAAEAKKEEKVEEKEESDDDMGFSLFD*