AlfalfaGEDB | Msa0127810

M. sativa cultivar zhongmu-4 / Msa0127810

KEGG: K02978
GO: GO:0000028 GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0015935 GO:0016020 GO:0016043 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0022626 GO:0022627 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042254 GO:0042255 GO:0042274 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0065003 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF01667.20

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0127810	XP_010482041.1	93.478	92	6	0	8	99	46	137	2.57e-56	180

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0127810	sp\|Q9M2F1\|RS272_ARATH	96.471	85	3	0	15	99	2	86	1.10e-57	175

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0127810	A0A151TEH8	96.552	87	3	0	13	99	1	87	3.50e-56	177

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0019420	Msa0127810	0.812252	4.655446e-51	-8.615850e-47
Msa0094940	Msa0127810	0.802690	4.978227e-49	-8.615850e-47
Msa0127810	Msa0189080	0.818821	1.604440e-52	-8.615850e-47
Msa0127810	Msa0226620	0.802307	5.970604e-49	-8.615850e-47
Msa0127810	Msa0231290	0.835006	2.156286e-56	-8.615850e-47
Msa0127810	Msa0260270	0.825415	4.746750e-54	-8.615850e-47
Msa0127810	Msa0304270	0.824920	6.215616e-54	-8.615850e-47
Msa0127810	Msa0612840	0.809350	1.975804e-50	-8.615850e-47
Msa0127810	Msa0884620	-0.812689	3.736533e-51	-8.615850e-47
Msa0127810	Msa0968320	0.820074	8.313297e-53	-8.615850e-47
Msa0127810	Msa1315510	0.846280	2.399089e-59	-8.615850e-47
Msa0127810	Msa1390390	0.837583	4.771873e-57	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0127810	MtrunA17_Chr2g0291901	100.000	85	0	15	99	2	86	8.33e-60	177
Msa0127810	MtrunA17_Chr1g0210581	100.000	85	0	15	99	2	86	8.33e-60	177
Msa0127810	MtrunA17_Chr2g0283551	98.824	85	1	15	99	2	86	1.90e-59	176

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0127810	AT3G61110.1	96.471	85	3	15	99	2	86	1.12e-58	175
Msa0127810	AT2G45710.1	95.181	83	4	15	97	2	84	1.05e-55	167
Msa0127810	AT5G47930.1	96.386	83	3	15	97	2	84	2.85e-55	166
Msa0127810	AT3G61111.1	83.529	85	14	15	99	2	86	1.35e-48	149
Msa0127810	AT3G61111.2	80.488	41	8	15	55	2	42	1.11e-17	71.2

Find 45 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATTATTTAATGACAAATTT+TGG	0.169262	1_3:+57410270	Msa0127810:three_prime_UTR
TCCACAGCCCTCTCAACTTT+GGG	0.311830	1_3:-57408902	None:intergenic
TAACAGAGGGGTGCTCCTTC+AGG	0.320285	1_3:+57410186	Msa0127810:CDS
TCAAGAGATCGATGTCGTTT+TGG	0.325370	1_3:-57409237	None:intergenic
ATCTCCTAACTCATTCTTCA+TGG	0.338607	1_3:+57409312	Msa0127810:CDS
TTGCTTTGCCACCGGTTGGC+TGG	0.350949	1_3:-57410160	None:intergenic
TGAGTAAATTTGGTTTGATA+TGG	0.363323	1_3:+57410375	Msa0127810:three_prime_UTR
TTCCACAGCCCTCTCAACTT+TGG	0.365114	1_3:-57408903	None:intergenic
CACTAGTCATGCTAAATTAG+AGG	0.380828	1_3:-57410497	None:intergenic
GCATGACTAGTGTACTAGTT+TGG	0.382981	1_3:+57410508	Msa0127810:three_prime_UTR
GGAAGAAGGGCGATTAGTTA+TGG	0.392275	1_3:+57410207	Msa0127810:exon
AATACCATGAAGAATGAGTT+AGG	0.426658	1_3:-57409316	None:intergenic
AAATTTAATTTAGTGCTAAC+GGG	0.427882	1_3:-57410412	None:intergenic
CTTACATGTTGAAGCATCCT+TGG	0.445045	1_3:-57409452	None:intergenic
GGTATGGCTACATGTGTCAT+AGG	0.470144	1_3:+57410529	Msa0127810:three_prime_UTR
TGTTTGTATAGATCAGCCTT+GGG	0.470617	1_3:+57410555	Msa0127810:three_prime_UTR
TAAATTTAATTTAGTGCTAA+CGG	0.479815	1_3:-57410413	None:intergenic
ACACATTGACACAAGACAAC+TGG	0.489033	1_3:-57410323	None:intergenic
TGTTAGCCTTGCTTTGCCAC+CGG	0.490848	1_3:-57410168	None:intergenic
AGCCAACCGGTGGCAAAGCA+AGG	0.501626	1_3:+57410162	Msa0127810:CDS
TTGTTTGTATAGATCAGCCT+TGG	0.502480	1_3:+57410554	Msa0127810:three_prime_UTR
GGGGTGCTCCTTCAGGAAGA+AGG	0.503456	1_3:+57410193	Msa0127810:CDS
CACCAGCGGAACTTGAGAAA+AGG	0.505547	1_3:+57409263	Msa0127810:CDS
TCTCAAACAGTCGTGGTTTG+CGG	0.511373	1_3:+57410116	Msa0127810:CDS
TAAATTTGGTTTGATATGGT+TGG	0.518219	1_3:+57410379	Msa0127810:three_prime_UTR
AGCCTTGCTTTGCCACCGGT+TGG	0.526124	1_3:-57410164	None:intergenic
TGTTTGAGAGTGGCTAAATA+CGG	0.536688	1_3:-57410102	None:intergenic
TCCTACAGCAGAATAAAGAA+TGG	0.551285	1_3:-57410593	None:intergenic
TTATAGGATGTCAAATGCCA+AGG	0.569104	1_3:+57409435	Msa0127810:intron
ACTAGTGTACTAGTTTGGTA+TGG	0.573694	1_3:+57410513	Msa0127810:three_prime_UTR
GGGTGCTCCTTCAGGAAGAA+GGG	0.581280	1_3:+57410194	Msa0127810:CDS
GGTTTGCGGCAACTGTCAGA+CGG	0.592548	1_3:+57410130	Msa0127810:CDS
GGCAAAGCAAGGCTAACAGA+GGG	0.595874	1_3:+57410173	Msa0127810:CDS
GATGGGACCCAAAGTTGAGA+GGG	0.597213	1_3:+57408895	None:intergenic
ACCCAAAGTTGAGAGGGCTG+TGG	0.599911	1_3:+57408901	Msa0127810:CDS
CTAATCGCCCTTCTTCCTGA+AGG	0.607238	1_3:-57410201	None:intergenic
AGATGGGACCCAAAGTTGAG+AGG	0.608186	1_3:+57408894	None:intergenic
TAGCCACTCTCAAACAGTCG+TGG	0.620995	1_3:+57410109	Msa0127810:CDS
GGCTGTGGAATCAAATGTGG+AGG	0.628873	1_3:+57408916	Msa0127810:CDS
GCATATCCTCAAAAGTCCCA+AGG	0.649139	1_3:-57410571	None:intergenic
TGGCAAAGCAAGGCTAACAG+AGG	0.657490	1_3:+57410172	Msa0127810:CDS
AAACCACGACTGTTTGAGAG+TGG	0.670924	1_3:-57410112	None:intergenic
GAGGGCTGTGGAATCAAATG+TGG	0.673501	1_3:+57408913	Msa0127810:CDS
CGATCTCTTGAATCCACCAG+CGG	0.707247	1_3:+57409249	Msa0127810:CDS
GCAAAGCAAGGCTAACAGAG+GGG	0.737903	1_3:+57410174	Msa0127810:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAAAACAAATTACAAA+CGG	-	chr1_3:57409388-57409407	None:intergenic	10.0%
!!!	TTATTATATTTCTATCATTT+AGG	+	chr1_3:57409810-57409829	Msa0127810:intron	10.0%
!!	AAAAAAAAACAAATTACAAA+CGG	-	chr1_3:57409388-57409407	None:intergenic	10.0%
!!!	TTATTATATTTCTATCATTT+AGG	+	chr1_3:57409810-57409829	Msa0127810:intron	10.0%
!!	TAAATGGAAAATCTATTTAA+AGG	-	chr1_3:57409019-57409038	None:intergenic	15.0%
!!	AATTAACATATACATAACAA+TGG	-	chr1_3:57409357-57409376	None:intergenic	15.0%
!!!	AAATTACTTTTTTATTCAAC+CGG	-	chr1_3:57409547-57409566	None:intergenic	15.0%
!!	TAATCTATCATTTAAATTGA+TGG	+	chr1_3:57409722-57409741	Msa0127810:intron	15.0%
!!	TTTATAATTGTTGAAATTTG+TGG	+	chr1_3:57410068-57410087	Msa0127810:intron	15.0%
!!!	GATTATTTAATGACAAATTT+TGG	+	chr1_3:57410270-57410289	Msa0127810:three_prime_UTR	15.0%
!!!	TAAATTTAATTTAGTGCTAA+CGG	-	chr1_3:57410416-57410435	None:intergenic	15.0%
!!	TAAATGGAAAATCTATTTAA+AGG	-	chr1_3:57409019-57409038	None:intergenic	15.0%
!!	AATTAACATATACATAACAA+TGG	-	chr1_3:57409357-57409376	None:intergenic	15.0%
!!!	AAATTACTTTTTTATTCAAC+CGG	-	chr1_3:57409547-57409566	None:intergenic	15.0%
!!	TAATCTATCATTTAAATTGA+TGG	+	chr1_3:57409722-57409741	Msa0127810:intron	15.0%
!!	TTTATAATTGTTGAAATTTG+TGG	+	chr1_3:57410068-57410087	Msa0127810:intron	15.0%
!!!	GATTATTTAATGACAAATTT+TGG	+	chr1_3:57410270-57410289	Msa0127810:three_prime_UTR	15.0%
!!!	TAAATTTAATTTAGTGCTAA+CGG	-	chr1_3:57410416-57410435	None:intergenic	15.0%
!!!	TATATCATATCTTTTTCACA+TGG	+	chr1_3:57408962-57408981	Msa0127810:intron	20.0%
!!!	ATATCATATCTTTTTCACAT+GGG	+	chr1_3:57408963-57408982	Msa0127810:intron	20.0%
!!	AAAAGACTGTAAATGTTAAA+TGG	-	chr1_3:57409035-57409054	None:intergenic	20.0%
!!!	TTTCTCTTTGTATTTGTTAT+AGG	+	chr1_3:57409419-57409438	Msa0127810:intron	20.0%
!!!	AATTACTTTTTTATTCAACC+GGG	-	chr1_3:57409546-57409565	None:intergenic	20.0%
!!	GGTTTATTCAACTAAAAAAA+TGG	-	chr1_3:57409608-57409627	None:intergenic	20.0%
!!!	TAGTTCATTGACTAAATTTA+AGG	-	chr1_3:57409629-57409648	None:intergenic	20.0%
!!	TTATTATACTTATACAACTC+AGG	-	chr1_3:57409672-57409691	None:intergenic	20.0%
!!	TATAAGTATAATAAACAGTC+CGG	+	chr1_3:57409678-57409697	Msa0127810:intron	20.0%
!!	TGACTAAATTAATAGAATTC+CGG	-	chr1_3:57409700-57409719	None:intergenic	20.0%
!!!	TATGCATATTTTTATCGTTT+AGG	+	chr1_3:57409749-57409768	Msa0127810:intron	20.0%
!!!	TTTGACTTGTTAGTTTATAA+TGG	+	chr1_3:57409930-57409949	Msa0127810:intron	20.0%
!!	TTTATAATGGTTGAAATTTG+TGG	+	chr1_3:57409943-57409962	Msa0127810:intron	20.0%
!!!	AATTTGATTTCTACTTTGAA+GGG	-	chr1_3:57409993-57410012	None:intergenic	20.0%
!!!	AAATTTGATTTCTACTTTGA+AGG	-	chr1_3:57409994-57410013	None:intergenic	20.0%
!!	AATTGAAACCAACTTATTAT+AGG	+	chr1_3:57410022-57410041	Msa0127810:intron	20.0%
!!!	AAATTTAATTTAGTGCTAAC+GGG	-	chr1_3:57410415-57410434	None:intergenic	20.0%
!!!	TATATCATATCTTTTTCACA+TGG	+	chr1_3:57408962-57408981	Msa0127810:intron	20.0%
!!!	ATATCATATCTTTTTCACAT+GGG	+	chr1_3:57408963-57408982	Msa0127810:intron	20.0%
!!	AAAAGACTGTAAATGTTAAA+TGG	-	chr1_3:57409035-57409054	None:intergenic	20.0%
!!!	TTTCTCTTTGTATTTGTTAT+AGG	+	chr1_3:57409419-57409438	Msa0127810:intron	20.0%
!!!	AATTACTTTTTTATTCAACC+GGG	-	chr1_3:57409546-57409565	None:intergenic	20.0%
!!	GGTTTATTCAACTAAAAAAA+TGG	-	chr1_3:57409608-57409627	None:intergenic	20.0%
!!!	TAGTTCATTGACTAAATTTA+AGG	-	chr1_3:57409629-57409648	None:intergenic	20.0%
!!	TTATTATACTTATACAACTC+AGG	-	chr1_3:57409672-57409691	None:intergenic	20.0%
!!	TATAAGTATAATAAACAGTC+CGG	+	chr1_3:57409678-57409697	Msa0127810:intron	20.0%
!!	TGACTAAATTAATAGAATTC+CGG	-	chr1_3:57409700-57409719	None:intergenic	20.0%
!!!	TATGCATATTTTTATCGTTT+AGG	+	chr1_3:57409749-57409768	Msa0127810:intron	20.0%
!!!	TTTGACTTGTTAGTTTATAA+TGG	+	chr1_3:57409930-57409949	Msa0127810:intron	20.0%
!!	TTTATAATGGTTGAAATTTG+TGG	+	chr1_3:57409943-57409962	Msa0127810:intron	20.0%
!!!	AATTTGATTTCTACTTTGAA+GGG	-	chr1_3:57409993-57410012	None:intergenic	20.0%
!!!	AAATTTGATTTCTACTTTGA+AGG	-	chr1_3:57409994-57410013	None:intergenic	20.0%
!!	AATTGAAACCAACTTATTAT+AGG	+	chr1_3:57410022-57410041	Msa0127810:intron	20.0%
!!!	AAATTTAATTTAGTGCTAAC+GGG	-	chr1_3:57410415-57410434	None:intergenic	20.0%
!	AATCTCATAAAGAGTTTCTA+GGG	+	chr1_3:57409081-57409100	Msa0127810:intron	25.0%
!	TAAAGAAACAAAAAAGAGAG+TGG	-	chr1_3:57409147-57409166	None:intergenic	25.0%
!	CAAACTTAATCAATCAGAAT+TGG	-	chr1_3:57409193-57409212	None:intergenic	25.0%
!!!	TTGATGATTTTGTTGTTTTC+AGG	+	chr1_3:57409210-57409229	Msa0127810:intron	25.0%
!!!	ATAAAACTCTAGTCTTTTTG+AGG	+	chr1_3:57410237-57410256	Msa0127810:three_prime_UTR	25.0%
!!!	TAAAACTCTAGTCTTTTTGA+GGG	+	chr1_3:57410238-57410257	Msa0127810:three_prime_UTR	25.0%
!!!	GAGAAGATTTTGAGTAAATT+TGG	+	chr1_3:57410365-57410384	Msa0127810:three_prime_UTR	25.0%
!!!	TGAGTAAATTTGGTTTGATA+TGG	+	chr1_3:57410375-57410394	Msa0127810:three_prime_UTR	25.0%
!!!	TAAATTTGGTTTGATATGGT+TGG	+	chr1_3:57410379-57410398	Msa0127810:three_prime_UTR	25.0%
!	AATCTCATAAAGAGTTTCTA+GGG	+	chr1_3:57409081-57409100	Msa0127810:intron	25.0%
!	TAAAGAAACAAAAAAGAGAG+TGG	-	chr1_3:57409147-57409166	None:intergenic	25.0%
!	CAAACTTAATCAATCAGAAT+TGG	-	chr1_3:57409193-57409212	None:intergenic	25.0%
!!!	TTGATGATTTTGTTGTTTTC+AGG	+	chr1_3:57409210-57409229	Msa0127810:intron	25.0%
!!!	ATAAAACTCTAGTCTTTTTG+AGG	+	chr1_3:57410237-57410256	Msa0127810:three_prime_UTR	25.0%
!!!	TAAAACTCTAGTCTTTTTGA+GGG	+	chr1_3:57410238-57410257	Msa0127810:three_prime_UTR	25.0%
!!!	GAGAAGATTTTGAGTAAATT+TGG	+	chr1_3:57410365-57410384	Msa0127810:three_prime_UTR	25.0%
!!!	TGAGTAAATTTGGTTTGATA+TGG	+	chr1_3:57410375-57410394	Msa0127810:three_prime_UTR	25.0%
!!!	TAAATTTGGTTTGATATGGT+TGG	+	chr1_3:57410379-57410398	Msa0127810:three_prime_UTR	25.0%
	AACTCTTTATGAGATTGGAA+GGG	-	chr1_3:57409077-57409096	None:intergenic	30.0%
	AAACTCTTTATGAGATTGGA+AGG	-	chr1_3:57409078-57409097	None:intergenic	30.0%
	CTAGAAACTCTTTATGAGAT+TGG	-	chr1_3:57409082-57409101	None:intergenic	30.0%
	CAATCTCATAAAGAGTTTCT+AGG	+	chr1_3:57409080-57409099	Msa0127810:intron	30.0%
	AATACCATGAAGAATGAGTT+AGG	-	chr1_3:57409319-57409338	None:intergenic	30.0%
	TTATATAGATACTAGAGACC+CGG	+	chr1_3:57409525-57409544	Msa0127810:intron	30.0%
	CAATGTCACAATGATTGTTA+TGG	+	chr1_3:57409774-57409793	Msa0127810:intron	30.0%
!	TCCTGTATAAGTCGAATTTT+TGG	+	chr1_3:57409870-57409889	Msa0127810:intron	30.0%
	ACCAAAAATTCGACTTATAC+AGG	-	chr1_3:57409874-57409893	None:intergenic	30.0%
!	CTTTGAAGGGAAAATAAATG+TGG	-	chr1_3:57409980-57409999	None:intergenic	30.0%
!!	AAAGGTGTCCTATAATAAGT+TGG	-	chr1_3:57410033-57410052	None:intergenic	30.0%
	AAACCAACAAGTCAAATCAA+AGG	-	chr1_3:57410051-57410070	None:intergenic	30.0%
!!	AAAACTCTAGTCTTTTTGAG+GGG	+	chr1_3:57410239-57410258	Msa0127810:three_prime_UTR	30.0%
	AACTCTTTATGAGATTGGAA+GGG	-	chr1_3:57409077-57409096	None:intergenic	30.0%
	AAACTCTTTATGAGATTGGA+AGG	-	chr1_3:57409078-57409097	None:intergenic	30.0%
	CTAGAAACTCTTTATGAGAT+TGG	-	chr1_3:57409082-57409101	None:intergenic	30.0%
	CAATCTCATAAAGAGTTTCT+AGG	+	chr1_3:57409080-57409099	Msa0127810:intron	30.0%
	AATACCATGAAGAATGAGTT+AGG	-	chr1_3:57409319-57409338	None:intergenic	30.0%
	TTATATAGATACTAGAGACC+CGG	+	chr1_3:57409525-57409544	Msa0127810:intron	30.0%
	CAATGTCACAATGATTGTTA+TGG	+	chr1_3:57409774-57409793	Msa0127810:intron	30.0%
!	TCCTGTATAAGTCGAATTTT+TGG	+	chr1_3:57409870-57409889	Msa0127810:intron	30.0%
	ACCAAAAATTCGACTTATAC+AGG	-	chr1_3:57409874-57409893	None:intergenic	30.0%
!	CTTTGAAGGGAAAATAAATG+TGG	-	chr1_3:57409980-57409999	None:intergenic	30.0%
!!	AAAGGTGTCCTATAATAAGT+TGG	-	chr1_3:57410033-57410052	None:intergenic	30.0%
	AAACCAACAAGTCAAATCAA+AGG	-	chr1_3:57410051-57410070	None:intergenic	30.0%
!!	AAAACTCTAGTCTTTTTGAG+GGG	+	chr1_3:57410239-57410258	Msa0127810:three_prime_UTR	30.0%
!	TTTTTCACATGGGCTCTTAA+TGG	+	chr1_3:57408973-57408992	Msa0127810:intron	35.0%
	ACTCTTTATGAGATTGGAAG+GGG	-	chr1_3:57409076-57409095	None:intergenic	35.0%
	ATCTCCTAACTCATTCTTCA+TGG	+	chr1_3:57409312-57409331	Msa0127810:CDS	35.0%
	TTATAGGATGTCAAATGCCA+AGG	+	chr1_3:57409435-57409454	Msa0127810:intron	35.0%
!!	ACACCTTTGATTTGACTTGT+TGG	+	chr1_3:57410045-57410064	Msa0127810:intron	35.0%
	TGTTTGAGAGTGGCTAAATA+CGG	-	chr1_3:57410105-57410124	None:intergenic	35.0%
	CACTAGTCATGCTAAATTAG+AGG	-	chr1_3:57410500-57410519	None:intergenic	35.0%
!	ACTAGTGTACTAGTTTGGTA+TGG	+	chr1_3:57410513-57410532	Msa0127810:three_prime_UTR	35.0%
	TTGTTTGTATAGATCAGCCT+TGG	+	chr1_3:57410554-57410573	Msa0127810:three_prime_UTR	35.0%
	TGTTTGTATAGATCAGCCTT+GGG	+	chr1_3:57410555-57410574	Msa0127810:three_prime_UTR	35.0%
!	TTTTTCACATGGGCTCTTAA+TGG	+	chr1_3:57408973-57408992	Msa0127810:intron	35.0%
	ACTCTTTATGAGATTGGAAG+GGG	-	chr1_3:57409076-57409095	None:intergenic	35.0%
	ATCTCCTAACTCATTCTTCA+TGG	+	chr1_3:57409312-57409331	Msa0127810:CDS	35.0%
	TTATAGGATGTCAAATGCCA+AGG	+	chr1_3:57409435-57409454	Msa0127810:intron	35.0%
!!	ACACCTTTGATTTGACTTGT+TGG	+	chr1_3:57410045-57410064	Msa0127810:intron	35.0%
	TGTTTGAGAGTGGCTAAATA+CGG	-	chr1_3:57410105-57410124	None:intergenic	35.0%
	CACTAGTCATGCTAAATTAG+AGG	-	chr1_3:57410500-57410519	None:intergenic	35.0%
!	ACTAGTGTACTAGTTTGGTA+TGG	+	chr1_3:57410513-57410532	Msa0127810:three_prime_UTR	35.0%
	TTGTTTGTATAGATCAGCCT+TGG	+	chr1_3:57410554-57410573	Msa0127810:three_prime_UTR	35.0%
	TGTTTGTATAGATCAGCCTT+GGG	+	chr1_3:57410555-57410574	Msa0127810:three_prime_UTR	35.0%
	CAATCAGAATTGGAGCGATT+CGG	-	chr1_3:57409183-57409202	None:intergenic	40.0%
!!	TCAAGAGATCGATGTCGTTT+TGG	-	chr1_3:57409240-57409259	None:intergenic	40.0%
!	CTTACATGTTGAAGCATCCT+TGG	-	chr1_3:57409455-57409474	None:intergenic	40.0%
	ACACATTGACACAAGACAAC+TGG	-	chr1_3:57410326-57410345	None:intergenic	40.0%
	GCATGACTAGTGTACTAGTT+TGG	+	chr1_3:57410508-57410527	Msa0127810:three_prime_UTR	40.0%
	CAATCAGAATTGGAGCGATT+CGG	-	chr1_3:57409183-57409202	None:intergenic	40.0%
!!	TCAAGAGATCGATGTCGTTT+TGG	-	chr1_3:57409240-57409259	None:intergenic	40.0%
!	CTTACATGTTGAAGCATCCT+TGG	-	chr1_3:57409455-57409474	None:intergenic	40.0%
	ACACATTGACACAAGACAAC+TGG	-	chr1_3:57410326-57410345	None:intergenic	40.0%
	GCATGACTAGTGTACTAGTT+TGG	+	chr1_3:57410508-57410527	Msa0127810:three_prime_UTR	40.0%
	AGGCGACTCTCACTCATATT+GGG	-	chr1_3:57408999-57409018	None:intergenic	45.0%
	AAGGCGACTCTCACTCATAT+TGG	-	chr1_3:57409000-57409019	None:intergenic	45.0%
	TCATCTCTCAACCTCGACAA+TGG	+	chr1_3:57409117-57409136	Msa0127810:intron	45.0%
	AGAGTGGTTAACCATTGTCG+AGG	-	chr1_3:57409131-57409150	None:intergenic	45.0%
!	TTCCTTTTCTCAAGTTCCGC+TGG	-	chr1_3:57409268-57409287	None:intergenic	45.0%
	AAACCACGACTGTTTGAGAG+TGG	-	chr1_3:57410115-57410134	None:intergenic	45.0%
	TCTCAAACAGTCGTGGTTTG+CGG	+	chr1_3:57410116-57410135	Msa0127810:CDS	45.0%
!	GGAAGAAGGGCGATTAGTTA+TGG	+	chr1_3:57410207-57410226	Msa0127810:exon	45.0%
	GGTATGGCTACATGTGTCAT+AGG	+	chr1_3:57410529-57410548	Msa0127810:three_prime_UTR	45.0%
	GCATATCCTCAAAAGTCCCA+AGG	-	chr1_3:57410574-57410593	None:intergenic	45.0%
	AGGCGACTCTCACTCATATT+GGG	-	chr1_3:57408999-57409018	None:intergenic	45.0%
	AAGGCGACTCTCACTCATAT+TGG	-	chr1_3:57409000-57409019	None:intergenic	45.0%
	TCATCTCTCAACCTCGACAA+TGG	+	chr1_3:57409117-57409136	Msa0127810:intron	45.0%
	AGAGTGGTTAACCATTGTCG+AGG	-	chr1_3:57409131-57409150	None:intergenic	45.0%
!	TTCCTTTTCTCAAGTTCCGC+TGG	-	chr1_3:57409268-57409287	None:intergenic	45.0%
	AAACCACGACTGTTTGAGAG+TGG	-	chr1_3:57410115-57410134	None:intergenic	45.0%
	TCTCAAACAGTCGTGGTTTG+CGG	+	chr1_3:57410116-57410135	Msa0127810:CDS	45.0%
!	GGAAGAAGGGCGATTAGTTA+TGG	+	chr1_3:57410207-57410226	Msa0127810:exon	45.0%
	GGTATGGCTACATGTGTCAT+AGG	+	chr1_3:57410529-57410548	Msa0127810:three_prime_UTR	45.0%
	GCATATCCTCAAAAGTCCCA+AGG	-	chr1_3:57410574-57410593	None:intergenic	45.0%
	TCCACAGCCCTCTCAACTTT+GGG	-	chr1_3:57408905-57408924	None:intergenic	50.0%
	TTCCACAGCCCTCTCAACTT+TGG	-	chr1_3:57408906-57408925	None:intergenic	50.0%
	GAGGGCTGTGGAATCAAATG+TGG	+	chr1_3:57408913-57408932	Msa0127810:CDS	50.0%
	GGCTGTGGAATCAAATGTGG+AGG	+	chr1_3:57408916-57408935	Msa0127810:CDS	50.0%
!	TCAGAATTGGAGCGATTCGG+AGG	-	chr1_3:57409180-57409199	None:intergenic	50.0%
	CGATCTCTTGAATCCACCAG+CGG	+	chr1_3:57409249-57409268	Msa0127810:CDS	50.0%
!	CTTTTCTCAAGTTCCGCTGG+TGG	-	chr1_3:57409265-57409284	None:intergenic	50.0%
	CACCAGCGGAACTTGAGAAA+AGG	+	chr1_3:57409263-57409282	Msa0127810:CDS	50.0%
	TAGCCACTCTCAAACAGTCG+TGG	+	chr1_3:57410109-57410128	Msa0127810:CDS	50.0%
!!	TGTTAGCCTTGCTTTGCCAC+CGG	-	chr1_3:57410171-57410190	None:intergenic	50.0%
	TGGCAAAGCAAGGCTAACAG+AGG	+	chr1_3:57410172-57410191	Msa0127810:CDS	50.0%
	GGCAAAGCAAGGCTAACAGA+GGG	+	chr1_3:57410173-57410192	Msa0127810:CDS	50.0%
	GCAAAGCAAGGCTAACAGAG+GGG	+	chr1_3:57410174-57410193	Msa0127810:CDS	50.0%
	CTAATCGCCCTTCTTCCTGA+AGG	-	chr1_3:57410204-57410223	None:intergenic	50.0%
!!	CTCTAGTCTTTTTGAGGGGC+TGG	+	chr1_3:57410243-57410262	Msa0127810:three_prime_UTR	50.0%
!	GATCAGCCTTGGGACTTTTG+AGG	+	chr1_3:57410565-57410584	Msa0127810:three_prime_UTR	50.0%
	TCCACAGCCCTCTCAACTTT+GGG	-	chr1_3:57408905-57408924	None:intergenic	50.0%
	TTCCACAGCCCTCTCAACTT+TGG	-	chr1_3:57408906-57408925	None:intergenic	50.0%
	GAGGGCTGTGGAATCAAATG+TGG	+	chr1_3:57408913-57408932	Msa0127810:CDS	50.0%
	GGCTGTGGAATCAAATGTGG+AGG	+	chr1_3:57408916-57408935	Msa0127810:CDS	50.0%
!	TCAGAATTGGAGCGATTCGG+AGG	-	chr1_3:57409180-57409199	None:intergenic	50.0%
	CGATCTCTTGAATCCACCAG+CGG	+	chr1_3:57409249-57409268	Msa0127810:CDS	50.0%
!	CTTTTCTCAAGTTCCGCTGG+TGG	-	chr1_3:57409265-57409284	None:intergenic	50.0%
	CACCAGCGGAACTTGAGAAA+AGG	+	chr1_3:57409263-57409282	Msa0127810:CDS	50.0%
	TAGCCACTCTCAAACAGTCG+TGG	+	chr1_3:57410109-57410128	Msa0127810:CDS	50.0%
!!	TGTTAGCCTTGCTTTGCCAC+CGG	-	chr1_3:57410171-57410190	None:intergenic	50.0%
	TGGCAAAGCAAGGCTAACAG+AGG	+	chr1_3:57410172-57410191	Msa0127810:CDS	50.0%
	GGCAAAGCAAGGCTAACAGA+GGG	+	chr1_3:57410173-57410192	Msa0127810:CDS	50.0%
	GCAAAGCAAGGCTAACAGAG+GGG	+	chr1_3:57410174-57410193	Msa0127810:CDS	50.0%
	CTAATCGCCCTTCTTCCTGA+AGG	-	chr1_3:57410204-57410223	None:intergenic	50.0%
!!	CTCTAGTCTTTTTGAGGGGC+TGG	+	chr1_3:57410243-57410262	Msa0127810:three_prime_UTR	50.0%
!	GATCAGCCTTGGGACTTTTG+AGG	+	chr1_3:57410565-57410584	Msa0127810:three_prime_UTR	50.0%
	ACCCAAAGTTGAGAGGGCTG+TGG	+	chr1_3:57408901-57408920	Msa0127810:CDS	55.0%
	GGTTTGCGGCAACTGTCAGA+CGG	+	chr1_3:57410130-57410149	Msa0127810:CDS	55.0%
!!	ACGGTTTTGTGCCAGCCAAC+CGG	+	chr1_3:57410149-57410168	Msa0127810:CDS	55.0%
!!	TAACAGAGGGGTGCTCCTTC+AGG	+	chr1_3:57410186-57410205	Msa0127810:CDS	55.0%
	GGGTGCTCCTTCAGGAAGAA+GGG	+	chr1_3:57410194-57410213	Msa0127810:CDS	55.0%
	ACCCAAAGTTGAGAGGGCTG+TGG	+	chr1_3:57408901-57408920	Msa0127810:CDS	55.0%
	GGTTTGCGGCAACTGTCAGA+CGG	+	chr1_3:57410130-57410149	Msa0127810:CDS	55.0%
!!	ACGGTTTTGTGCCAGCCAAC+CGG	+	chr1_3:57410149-57410168	Msa0127810:CDS	55.0%
!!	TAACAGAGGGGTGCTCCTTC+AGG	+	chr1_3:57410186-57410205	Msa0127810:CDS	55.0%
	GGGTGCTCCTTCAGGAAGAA+GGG	+	chr1_3:57410194-57410213	Msa0127810:CDS	55.0%
!	GTTTTGTGCCAGCCAACCGG+TGG	+	chr1_3:57410152-57410171	Msa0127810:CDS	60.0%
!	TTGCTTTGCCACCGGTTGGC+TGG	-	chr1_3:57410163-57410182	None:intergenic	60.0%
	AGCCAACCGGTGGCAAAGCA+AGG	+	chr1_3:57410162-57410181	Msa0127810:CDS	60.0%
	AGCCTTGCTTTGCCACCGGT+TGG	-	chr1_3:57410167-57410186	None:intergenic	60.0%
!	GGGGTGCTCCTTCAGGAAGA+AGG	+	chr1_3:57410193-57410212	Msa0127810:CDS	60.0%
!	GTTTTGTGCCAGCCAACCGG+TGG	+	chr1_3:57410152-57410171	Msa0127810:CDS	60.0%
!	TTGCTTTGCCACCGGTTGGC+TGG	-	chr1_3:57410163-57410182	None:intergenic	60.0%
	AGCCAACCGGTGGCAAAGCA+AGG	+	chr1_3:57410162-57410181	Msa0127810:CDS	60.0%
	AGCCTTGCTTTGCCACCGGT+TGG	-	chr1_3:57410167-57410186	None:intergenic	60.0%
!	GGGGTGCTCCTTCAGGAAGA+AGG	+	chr1_3:57410193-57410212	Msa0127810:CDS	60.0%
	AAGGGGCTGCGACTGCGAGA+AGG	-	chr1_3:57409059-57409078	None:intergenic	65.0%
	AAGGGGCTGCGACTGCGAGA+AGG	-	chr1_3:57409059-57409078	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	57408896	57410600	57408896	ID=Msa0127810;Name=Msa0127810
chr1_3	mRNA	57408896	57410600	57408896	ID=Msa0127810-mRNA-1;Parent=Msa0127810;Name=Msa0127810-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0\|0.66\|0.75\|0.75\|1\|1\|4\|377\|99
chr1_3	exon	57408896	57408937	57408896	ID=Msa0127810-mRNA-1:exon:18221;Parent=Msa0127810-mRNA-1
chr1_3	exon	57409232	57409333	57409232	ID=Msa0127810-mRNA-1:exon:18222;Parent=Msa0127810-mRNA-1
chr1_3	exon	57409441	57409469	57409441	ID=Msa0127810-mRNA-1:exon:18223;Parent=Msa0127810-mRNA-1
chr1_3	exon	57410097	57410600	57410097	ID=Msa0127810-mRNA-1:exon:18224;Parent=Msa0127810-mRNA-1
chr1_3	CDS	57408896	57408937	57408896	ID=Msa0127810-mRNA-1:cds;Parent=Msa0127810-mRNA-1
chr1_3	CDS	57409232	57409333	57409232	ID=Msa0127810-mRNA-1:cds;Parent=Msa0127810-mRNA-1
chr1_3	CDS	57409441	57409469	57409441	ID=Msa0127810-mRNA-1:cds;Parent=Msa0127810-mRNA-1
chr1_3	CDS	57410097	57410223	57410097	ID=Msa0127810-mRNA-1:cds;Parent=Msa0127810-mRNA-1
chr1_3	three_prime_UTR	57410224	57410600	57410224	ID=Msa0127810-mRNA-1:three_prime_utr;Parent=Msa0127810-mRNA-1

Gene Sequence	>Msa0127810 ATGGGACCCAAAGTTGAGAGGGCTGTGGAATCAAATGTGGAGGTTCTCCAAAACGACATCGATCTCTTGAATCCACCAGCGGAACTTGAGAAAAGGAAGCACAAGCTCAAGCGTCTCGTTCAATCTCCTAACTCATTCTTCATGGATGTCAAATGCCAAGGATGCTTCAACATAACTACCGTATTTAGCCACTCTCAAACAGTCGTGGTTTGCGGCAACTGTCAGACGGTTTTGTGCCAGCCAACCGGTGGCAAAGCAAGGCTAACAGAGGGGTGCTCCTTCAGGAAGAAGGGCGATTAG
Protein sequence	>Msa0127810 MGPKVERAVESNVEVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVCGNCQTVLCQPTGGKARLTEGCSFRKKGD*