AlfalfaGEDB | Msa0304270

M. sativa cultivar zhongmu-4 / Msa0304270

KEGG: K02265
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005746 GO:0005751 GO:0006091 GO:0006119 GO:0006123 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006839 GO:0008150 GO:0008152 GO:0009058 GO:0009060 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0015672 GO:0015980 GO:0015985 GO:0015986 GO:0016020 GO:0016310 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019646 GO:0019693 GO:0019866 GO:0022900 GO:0022904 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032991 GO:0034220 GO:0034641 GO:0034654 GO:0042773 GO:0042775 GO:0042776 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045277 GO:0045333 GO:0046034 GO:0046390 GO:0046483 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0055086 GO:0055114 GO:0070069 GO:0070469 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:0098655 GO:0098660 GO:0098662 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600 GO:1990542
PFAM: PF01215.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0304270	XP_003597841.1	99.333	150	1	0	1	150	1	150	2.69e-105	307

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0304270	sp\|Q9SSB8\|CX5B2_ARATH	61.392	158	42	3	1	140	1	157	5.55e-58	181

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003030	Msa0304270	0.802770	4.791077e-49	-8.615850e-47
Msa0036610	Msa0304270	0.823476	1.357215e-53	-8.615850e-47
Msa0051200	Msa0304270	-0.802391	5.736022e-49	-8.615850e-47
Msa0051460	Msa0304270	-0.805758	1.143792e-49	-8.615850e-47
Msa0077390	Msa0304270	-0.804192	2.430109e-49	-8.615850e-47
Msa0094940	Msa0304270	0.975847	1.037899e-140	-8.615850e-47
Msa0127810	Msa0304270	0.824920	6.215616e-54	-8.615850e-47
Msa0128320	Msa0304270	0.873870	1.051153e-67	-8.615850e-47
Msa0130430	Msa0304270	0.806955	6.395751e-50	-8.615850e-47
Msa0135590	Msa0304270	-0.822638	2.127765e-53	-8.615850e-47
Msa0146910	Msa0304270	0.804066	2.581569e-49	-8.615850e-47
Msa0167700	Msa0304270	0.815272	1.006636e-51	-8.615850e-47
Msa0189080	Msa0304270	0.824480	7.891819e-54	-8.615850e-47
Msa0226620	Msa0304270	0.950575	1.240140e-108	-8.615850e-47
Msa0231290	Msa0304270	0.843681	1.206389e-58	-8.615850e-47
Msa0260230	Msa0304270	0.857639	1.423664e-62	-8.615850e-47
Msa0260270	Msa0304270	0.968603	6.521707e-129	-8.615850e-47
Msa0304270	Msa0304310	0.980865	3.237487e-151	-8.615850e-47
Msa0304270	Msa0323420	0.818521	1.876971e-52	-8.615850e-47
Msa0304270	Msa0334170	-0.842274	2.858004e-58	-8.615850e-47
Msa0304270	Msa0377610	0.835223	1.901807e-56	-8.615850e-47
Msa0304270	Msa0379850	-0.823686	1.211805e-53	-8.615850e-47
Msa0304270	Msa0389360	-0.818009	2.451764e-52	-8.615850e-47
Msa0304270	Msa0401150	0.818371	2.029653e-52	-8.615850e-47
Msa0304270	Msa0426820	0.821044	4.977608e-53	-8.615850e-47
Msa0304270	Msa0429170	-0.813933	1.991723e-51	-8.615850e-47
Msa0304270	Msa0438050	-0.809303	2.022551e-50	-8.615850e-47
Msa0304270	Msa0467740	0.830714	2.513855e-55	-8.615850e-47
Msa0304270	Msa0470230	0.857878	1.209055e-62	-8.615850e-47
Msa0304270	Msa0514130	0.821111	4.804464e-53	-8.615850e-47
Msa0304270	Msa0527900	-0.832035	1.189501e-55	-8.615850e-47
Msa0304270	Msa0532810	0.809030	2.314529e-50	-8.615850e-47
Msa0304270	Msa0543900	-0.834625	2.689723e-56	-8.615850e-47
Msa0304270	Msa0544930	-0.806887	6.613610e-50	-8.615850e-47
Msa0304270	Msa0564510	-0.809004	2.344020e-50	-8.615850e-47
Msa0304270	Msa0599770	-0.826871	2.138952e-54	-8.615850e-47
Msa0304270	Msa0610750	0.852606	4.134954e-61	-8.615850e-47
Msa0304270	Msa0612840	0.871469	6.676350e-67	-8.615850e-47
Msa0304270	Msa0616560	0.805075	1.590167e-49	-8.615850e-47
Msa0304270	Msa0621170	0.859377	4.315486e-63	-8.615850e-47
Msa0304270	Msa0621190	0.835483	1.634196e-56	-8.615850e-47
Msa0304270	Msa0639670	0.814190	1.747999e-51	-8.615850e-47
Msa0304270	Msa0640380	-0.863561	2.280657e-64	-8.615850e-47
Msa0304270	Msa0641020	-0.819382	1.195840e-52	-8.615850e-47
Msa0304270	Msa0641030	-0.809151	2.180645e-50	-8.615850e-47
Msa0304270	Msa0648370	0.838109	3.494716e-57	-8.615850e-47
Msa0304270	Msa0678820	-0.826896	2.109207e-54	-8.615850e-47
Msa0304270	Msa0679560	-0.838373	2.989564e-57	-8.615850e-47
Msa0304270	Msa0692850	0.805392	1.364526e-49	-8.615850e-47
Msa0304270	Msa0700370	0.807474	4.965591e-50	-8.615850e-47
Msa0304270	Msa0706350	0.808296	3.321498e-50	-8.615850e-47
Msa0304270	Msa0723210	-0.815089	1.105195e-51	-8.615850e-47
Msa0304270	Msa0744500	-0.802804	4.715682e-49	-8.615850e-47
Msa0304270	Msa0777460	0.836855	7.326597e-57	-8.615850e-47
Msa0304270	Msa0807050	-0.812278	4.594840e-51	-8.615850e-47
Msa0304270	Msa0826650	-0.804826	1.792380e-49	-8.615850e-47
Msa0304270	Msa0832990	-0.812607	3.894452e-51	-8.615850e-47
Msa0304270	Msa0860760	-0.814245	1.699428e-51	-8.615850e-47
Msa0304270	Msa0864480	0.809062	2.278589e-50	-8.615850e-47
Msa0304270	Msa0864690	-0.809677	1.681427e-50	-8.615850e-47
Msa0304270	Msa0884620	-0.861199	1.213639e-63	-8.615850e-47
Msa0304270	Msa0886660	-0.803335	3.661499e-49	-8.615850e-47
Msa0304270	Msa0912230	-0.803469	3.434947e-49	-8.615850e-47
Msa0304270	Msa0962190	0.811369	7.248058e-51	-8.615850e-47
Msa0304270	Msa0996830	0.849875	2.441302e-60	-8.615850e-47
Msa0304270	Msa0998890	0.823105	1.656279e-53	-8.615850e-47
Msa0304270	Msa1001210	-0.807131	5.870917e-50	-8.615850e-47
Msa0304270	Msa1054410	-0.808603	2.856025e-50	-8.615850e-47
Msa0304270	Msa1068310	0.808826	2.559300e-50	-8.615850e-47
Msa0304270	Msa1080790	-0.828532	8.529964e-55	-8.615850e-47
Msa0304270	Msa1118930	-0.822467	2.332398e-53	-8.615850e-47
Msa0304270	Msa1119970	0.832147	1.115743e-55	-8.615850e-47
Msa0304270	Msa1132950	-0.810380	1.186400e-50	-8.615850e-47
Msa0304270	Msa1144630	0.806482	8.052240e-50	-8.615850e-47
Msa0304270	Msa1147690	0.840676	7.532518e-58	-8.615850e-47
Msa0304270	Msa1185870	0.836636	8.332713e-57	-8.615850e-47
Msa0304270	Msa1203360	-0.815818	7.606466e-52	-8.615850e-47
Msa0304270	Msa1205410	0.849044	4.162379e-60	-8.615850e-47
Msa0304270	Msa1209560	-0.824881	6.345548e-54	-8.615850e-47
Msa0304270	Msa1221210	0.817595	3.039043e-52	-8.615850e-47
Msa0304270	Msa1221480	0.823506	1.335569e-53	-8.615850e-47
Msa0304270	Msa1222060	0.816108	6.552531e-52	-8.615850e-47
Msa0304270	Msa1246040	-0.815604	8.491198e-52	-8.615850e-47
Msa0304270	Msa1279220	-0.820047	8.429925e-53	-8.615850e-47
Msa0304270	Msa1279690	0.805197	1.499254e-49	-8.615850e-47
Msa0304270	Msa1297430	0.838096	3.521660e-57	-8.615850e-47
Msa0304270	Msa1303200	0.819808	9.563503e-53	-8.615850e-47
Msa0304270	Msa1328830	0.831723	1.420299e-55	-8.615850e-47
Msa0304270	Msa1330530	-0.803107	4.082347e-49	-8.615850e-47
Msa0304270	Msa1334040	0.817614	3.009385e-52	-8.615850e-47
Msa0304270	Msa1363930	0.808190	3.498490e-50	-8.615850e-47
Msa0304270	Msa1370630	-0.810024	1.416041e-50	-8.615850e-47
Msa0304270	Msa1377070	-0.803246	3.819576e-49	-8.615850e-47
Msa0304270	Msa1387750	0.816501	5.353546e-52	-8.615850e-47
Msa0304270	Msa1390390	0.846125	2.643898e-59	-8.615850e-47
Msa0304270	Msa1437530	0.874384	7.042990e-68	-8.615850e-47
Msa0304270	Msa1445250	-0.822998	1.755097e-53	-8.615850e-47
Msa0304270	Msa1465890	0.814532	1.468319e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0304270	MtrunA17_Chr2g0332571	99.333	150	1	0	1	150	1	150	2.48e-109	307
Msa0304270	MtrunA17_Chr4g0003811	75.325	154	28	4	1	149	1	149	2.48e-75	221

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0304270	AT1G80230.1	61.392	158	42	3	1	140	1	157	5.64e-59	181
Msa0304270	AT3G15640.2	58.491	159	45	4	1	140	1	157	1.55e-52	164
Msa0304270	AT3G15640.1	57.862	159	47	3	1	140	1	158	1.04e-51	162
Msa0304270	AT1G52710.1	59.302	86	35	0	65	150	4	89	2.01e-33	113

Find 108 sgRNAs with CRISPR-Local

Find 327 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAGCGTTGCTATCAATTATT+AGG	0.262008	2_3:-61584381	None:intergenic
AGCATGATGTTGTCTGGTTT+TGG	0.271011	2_3:+61582827	Msa0304270:CDS
GAGAATGAGATGAAGAAGTT+AGG	0.298612	2_3:-61580160	None:intergenic
TGTCCCATGTTTGAATTCTA+AGG	0.300229	2_3:-61584548	None:intergenic
GGAAACTTTGGTTCCTTAAT+CGG	0.319479	2_3:+61584731	Msa0304270:three_prime_UTR
TCACGTTGCAATGCTTTGTT+TGG	0.352341	2_3:+61584250	Msa0304270:three_prime_UTR
GGAATGTAAAACAATCTCTT+TGG	0.352887	2_3:-61584215	None:intergenic
CGCGCTCAAGTTTAACTTCA+AGG	0.362659	2_3:-61579963	None:intergenic
ATTAAGCTACAAATCCTGTT+AGG	0.366895	2_3:+61584341	Msa0304270:three_prime_UTR
TTAAATTAAGAGGGAAACTT+TGG	0.368684	2_3:+61584719	Msa0304270:three_prime_UTR
GGGCACTCAAAATCCGATTA+AGG	0.369464	2_3:-61584744	None:intergenic
GACCACCCTGAAGGTCCTTT+TGG	0.374661	2_3:+61581096	Msa0304270:CDS
TTTGAGATCTACGGTGGTTA+CGG	0.386481	2_3:-61580092	None:intergenic
TTCGTGCCAAAAGGACCTTC+AGG	0.388454	2_3:-61581102	None:intergenic
AGTCAAGTCACAACAGTAAC+TGG	0.389318	2_3:-61584629	None:intergenic
CGGCGTCGTTTGATTGGAAA+TGG	0.390861	2_3:-61580072	None:intergenic
GGACCTGGCGGTGATCCTTA+TGG	0.392481	2_3:+61584147	Msa0304270:CDS
CCCGTCACGTGCTAATTCGT+TGG	0.399931	2_3:+61579939	Msa0304270:five_prime_UTR
CGTTCATGACCGGTAGCAAT+TGG	0.405824	2_3:-61580362	None:intergenic
TACTATAACAGAAGGATTGT+TGG	0.415818	2_3:+61582661	Msa0304270:CDS
GGCGACGTGGCGGCTGAGGC+CGG	0.418601	2_3:-61580216	None:intergenic
TATACACCCCACCATCAAAT+GGG	0.419953	2_3:+61579888	None:intergenic
CCGTCACGTGCTAATTCGTT+GGG	0.425977	2_3:+61579940	Msa0304270:five_prime_UTR
CTATTTGAAATTAAATTAAG+AGG	0.427196	2_3:+61584709	Msa0304270:three_prime_UTR
AGAATGAGATGAAGAAGTTA+GGG	0.429164	2_3:-61580159	None:intergenic
TGCACACACTGGACATTCAT+GGG	0.432638	2_3:-61582866	None:intergenic
TGGTTACGGCGTCGTTTGAT+TGG	0.435570	2_3:-61580078	None:intergenic
GTGCACACACTGGACATTCA+TGG	0.436671	2_3:-61582867	None:intergenic
TGTTGGATGCCCCGGTGGTG+AGG	0.446924	2_3:+61582678	Msa0304270:CDS
CGCCACGTCGCCACACAATC+AGG	0.449393	2_3:+61580227	Msa0304270:CDS
ATATACACCCCACCATCAAA+TGG	0.450852	2_3:+61579887	None:intergenic
CACTTACCACCCTCACCACC+GGG	0.453277	2_3:-61582688	None:intergenic
CTTCAGGGTGGTCTATTTCA+AGG	0.458207	2_3:-61581086	None:intergenic
GGCGGCTGAGGCCGGCGAAT+CGG	0.463992	2_3:-61580208	None:intergenic
ACACTTACCACCCTCACCAC+CGG	0.473884	2_3:-61582689	None:intergenic
GTTGTGACTTGACTGTTGGT+TGG	0.474792	2_3:+61584638	Msa0304270:three_prime_UTR
GTTGGATGCCCCGGTGGTGA+GGG	0.475405	2_3:+61582679	Msa0304270:CDS
TACTGTTGTGACTTGACTGT+TGG	0.480725	2_3:+61584634	Msa0304270:three_prime_UTR
TTTGGAAAATTTATTCCTGA+AGG	0.482002	2_3:-61584197	None:intergenic
GTGTGGCGACGTGGCGGCTG+AGG	0.482725	2_3:-61580220	None:intergenic
TCTGATCAATGGTGTCCATA+AGG	0.484112	2_3:-61584162	None:intergenic
TTACTAACCTTCGTGCCAAA+AGG	0.484133	2_3:-61581111	None:intergenic
CATCCTCGATCCGCCGTCGC+CGG	0.485063	2_3:+61580182	Msa0304270:CDS
AGCCTCCGCTTTGATCCATT+AGG	0.487703	2_3:+61584502	Msa0304270:three_prime_UTR
GGTTGTGATTGTAGCTGGTT+CGG	0.494202	2_3:+61580313	Msa0304270:intron
AGGATGAGCATGATGTTGTC+TGG	0.496376	2_3:+61582821	Msa0304270:CDS
GGCGAATCGGAGACCGGCGA+CGG	0.501941	2_3:-61580195	None:intergenic
CAGCTTGAAGTGGTGGGACC+TGG	0.505788	2_3:+61584132	Msa0304270:intron
TGCGTTACAGCTTGAAGTGG+TGG	0.516079	2_3:+61584125	Msa0304270:intron
GTCGCCGGTCTCCGATTCGC+CGG	0.516296	2_3:+61580197	Msa0304270:CDS
TTCTGTGATTATTTAGACAC+AGG	0.517900	2_3:+61584307	Msa0304270:three_prime_UTR
TGAAAATACTGTGCACACAC+TGG	0.523295	2_3:-61582877	None:intergenic
AGAAGGATTGTTGGATGCCC+CGG	0.526647	2_3:+61582670	Msa0304270:CDS
GTTTGATTGGAAATGGAAGA+AGG	0.531244	2_3:-61580065	None:intergenic
AAACTGCAGCATCTGATCAA+TGG	0.533620	2_3:-61584173	None:intergenic
TTTAGCACCTATCCACAAAA+GGG	0.535268	2_3:+61584457	Msa0304270:three_prime_UTR
AAACATCAGTTGGTGGATGA+AGG	0.536831	2_3:-61584589	None:intergenic
TCGTGCCAAAAGGACCTTCA+GGG	0.538959	2_3:-61581101	None:intergenic
TTAAGTCTTACTATAACAGA+AGG	0.543566	2_3:+61582653	Msa0304270:CDS
GAGGCCGGCGAATCGGAGAC+CGG	0.548914	2_3:-61580201	None:intergenic
AATTCAACACAAACATCAGT+TGG	0.549766	2_3:-61584599	None:intergenic
TAGTAAGACTTAACAACGGC+AGG	0.550387	2_3:-61582643	None:intergenic
GCGTTACAGCTTGAAGTGGT+GGG	0.554549	2_3:+61584126	Msa0304270:intron
AATCTCCTCACGTTCATGAC+CGG	0.554757	2_3:-61580372	None:intergenic
TATTTGAAATTAAATTAAGA+GGG	0.555579	2_3:+61584710	Msa0304270:three_prime_UTR
AACGATTCTCCGCCACATGA+TGG	0.566084	2_3:-61580117	None:intergenic
GGATGCCCCGGTGGTGAGGG+TGG	0.570608	2_3:+61582682	Msa0304270:CDS
GATGTAGTTCCAATTGCTAC+CGG	0.573966	2_3:+61580353	Msa0304270:CDS
TAGTCCTTAGAATTCAAACA+TGG	0.582114	2_3:+61584544	Msa0304270:three_prime_UTR
CCAACGAATTAGCACGTGAC+GGG	0.584689	2_3:-61579939	None:intergenic
GAATCGGAGACCGGCGACGG+CGG	0.589478	2_3:-61580192	None:intergenic
GGACATGTTAATCCCTCCGT+CGG	0.589725	2_3:+61584566	Msa0304270:three_prime_UTR
AGACCGGCGACGGCGGATCG+AGG	0.592370	2_3:-61580185	None:intergenic
GGTGGATGAAGGCCGACGGA+GGG	0.593535	2_3:-61584578	None:intergenic
TCAACACAAACATCAGTTGG+TGG	0.593833	2_3:-61584596	None:intergenic
TGTCCATAAGGATCACCGCC+AGG	0.601139	2_3:-61584150	None:intergenic
CACCTAATGGATCAAAGCGG+AGG	0.601580	2_3:-61584504	None:intergenic
TCTGTGATTATTTAGACACA+GGG	0.605541	2_3:+61584308	Msa0304270:three_prime_UTR
TAGGTGCTAAAAGTAGCTGC+AGG	0.612310	2_3:-61584445	None:intergenic
ATGATGGTGTTTGAGATCTA+CGG	0.616818	2_3:-61580101	None:intergenic
ATACACCCCACCATCAAATG+GGG	0.619440	2_3:+61579889	None:intergenic
TTCTGCGTTACAGCTTGAAG+TGG	0.625576	2_3:+61584122	Msa0304270:intron
TGGTGGATGAAGGCCGACGG+AGG	0.626007	2_3:-61584579	None:intergenic
TTTGACTGGTCTAGACCTTG+CGG	0.626127	2_3:+61584274	Msa0304270:three_prime_UTR
TGCTACCGGTCATGAACGTG+AGG	0.630864	2_3:+61580367	Msa0304270:CDS
TTCGGTGAAGAAGACAGTTG+AGG	0.641042	2_3:+61580331	Msa0304270:CDS
AGTTGGTGGATGAAGGCCGA+CGG	0.645942	2_3:-61584582	None:intergenic
GGAGATTCAAGCTGATCTTG+AGG	0.651732	2_3:+61580388	Msa0304270:CDS
AGTCCTTAGAATTCAAACAT+GGG	0.652398	2_3:+61584545	Msa0304270:three_prime_UTR
CAAGTCACAACAGTAACTGG+TGG	0.653149	2_3:-61584626	None:intergenic
CCCAACGAATTAGCACGTGA+CGG	0.656695	2_3:-61579940	None:intergenic
CTTGAAATAGACCACCCTGA+AGG	0.658668	2_3:+61581087	Msa0304270:CDS
GCACACACTGGACATTCATG+GGG	0.668546	2_3:-61582865	None:intergenic
TACCTGATTGTGTGGCGACG+TGG	0.669661	2_3:-61580229	None:intergenic
CAGAAACTCTCTGAACCGCA+AGG	0.670121	2_3:-61584289	None:intergenic
CTTGAAGTGGTGGGACCTGG+CGG	0.684429	2_3:+61584135	Msa0304270:CDS
AGATCTCAAACACCATCATG+TGG	0.684707	2_3:+61580105	Msa0304270:exon
AGTGAAGATACCTGATTGTG+TGG	0.687822	2_3:-61580237	None:intergenic
CTGATTGTGTGGCGACGTGG+CGG	0.691244	2_3:-61580226	None:intergenic
TACACCCCACCATCAAATGG+GGG	0.694244	2_3:+61579890	None:intergenic
TCTCAAACACCATCATGTGG+CGG	0.696333	2_3:+61580108	Msa0304270:exon
GTGATTATTTAGACACAGGG+TGG	0.700670	2_3:+61584311	Msa0304270:three_prime_UTR
AGGATTGTTGGATGCCCCGG+TGG	0.708518	2_3:+61582673	Msa0304270:CDS
TGCCAAAAGGACCTTCAGGG+TGG	0.711901	2_3:-61581098	None:intergenic
GTTATAGTAAGACTTAACAA+CGG	0.724381	2_3:-61582647	None:intergenic
ATGGTGTTTGAGATCTACGG+TGG	0.739505	2_3:-61580098	None:intergenic
TTTCACCTAATGGATCAAAG+CGG	0.742764	2_3:-61584507	None:intergenic
ACTTACCACCCTCACCACCG+GGG	0.751343	2_3:-61582687	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATTTGAAATTAAATTAAGA+GGG	+	chr2_3:61584710-61584729	Msa0304270:three_prime_UTR	10.0%
!!	ACACAAGTTATAAATAAAAA+TGG	-	chr2_3:61580519-61580538	None:intergenic	15.0%
!!!	TGATTTTAAATTTTAATCTG+AGG	+	chr2_3:61580646-61580665	Msa0304270:intron	15.0%
!!	CATTTAAAAACTTTAGTAAT+GGG	-	chr2_3:61580758-61580777	None:intergenic	15.0%
!!	ACATTTAAAAACTTTAGTAA+TGG	-	chr2_3:61580759-61580778	None:intergenic	15.0%
!!!	ACTAAAGTTTTTAAATGTTT+TGG	+	chr2_3:61580761-61580780	Msa0304270:intron	15.0%
!!!	CTAAAGTTTTTAAATGTTTT+GGG	+	chr2_3:61580762-61580781	Msa0304270:intron	15.0%
!!!	TAAAGTTTTTAAATGTTTTG+GGG	+	chr2_3:61580763-61580782	Msa0304270:intron	15.0%
!!!	TTTGTATTTTTTGTTTGTTT+AGG	+	chr2_3:61581053-61581072	Msa0304270:intron	15.0%
!!!	TTGTATTTTTTGTTTGTTTA+GGG	+	chr2_3:61581054-61581073	Msa0304270:intron	15.0%
!!!	AAAATCACTTTTCTAAATTA+AGG	-	chr2_3:61581301-61581320	None:intergenic	15.0%
!!!	TTAATTTAGAAAAGTGATTT+TGG	+	chr2_3:61581300-61581319	Msa0304270:intron	15.0%
!!	TTTATACTTTAATTACCTAA+AGG	+	chr2_3:61581370-61581389	Msa0304270:intron	15.0%
!!	TTATACTTTAATTACCTAAA+GGG	+	chr2_3:61581371-61581390	Msa0304270:intron	15.0%
!!!	TATTTAATCTTTTTGAAGTT+TGG	+	chr2_3:61581520-61581539	Msa0304270:intron	15.0%
!!	ATATATGATAGAACATTATA+GGG	+	chr2_3:61581631-61581650	Msa0304270:intron	15.0%
!!!	ATTTTTGAAGAATGATAATT+TGG	-	chr2_3:61581854-61581873	None:intergenic	15.0%
!!!	ATGTTTATAACTATTTTTAC+TGG	+	chr2_3:61582915-61582934	Msa0304270:intron	15.0%
!!	TAAAATAAATAGGAATATGT+TGG	-	chr2_3:61583288-61583307	None:intergenic	15.0%
!!	GAAAAAGATGTAAAATAAAT+AGG	-	chr2_3:61583298-61583317	None:intergenic	15.0%
!!	TAGAAAAAATTACTTGAAAA+TGG	+	chr2_3:61583936-61583955	Msa0304270:intron	15.0%
!!!	ATTTAACTAGAATTTGAAAT+TGG	-	chr2_3:61584003-61584022	None:intergenic	15.0%
!!!	CTATTTGAAATTAAATTAAG+AGG	+	chr2_3:61584709-61584728	Msa0304270:three_prime_UTR	15.0%
!!!	GATTTTGATGAATTTTGATT+TGG	+	chr2_3:61580292-61580311	Msa0304270:intron	20.0%
!!	ACAAAATGGAAAAATAAAAG+TGG	-	chr2_3:61580575-61580594	None:intergenic	20.0%
!!!	TTTAGTAATGGGATAATAAA+TGG	-	chr2_3:61580747-61580766	None:intergenic	20.0%
!!	TATTTCCAAAACTATAGTAA+TGG	+	chr2_3:61580825-61580844	Msa0304270:intron	20.0%
!!!	TTTGAAATAGCAACTATATT+GGG	-	chr2_3:61580889-61580908	None:intergenic	20.0%
!!!	TTTTGAAATAGCAACTATAT+TGG	-	chr2_3:61580890-61580909	None:intergenic	20.0%
!!!	CATATTATATAGGTTACTTT+TGG	+	chr2_3:61581010-61581029	Msa0304270:intron	20.0%
!!!	AATGGTTTTGGTTTATTTTT+TGG	+	chr2_3:61581272-61581291	Msa0304270:intron	20.0%
!!!	TGTGTTGTTAAAAATCATTA+TGG	+	chr2_3:61581344-61581363	Msa0304270:intron	20.0%
!!!	TTTTATCTTACAATTGAGTA+TGG	-	chr2_3:61581490-61581509	None:intergenic	20.0%
!!	CTCAATTGTAAGATAAAATA+TGG	+	chr2_3:61581492-61581511	Msa0304270:intron	20.0%
!!	AATAGTTTCTCTTTAAACTA+AGG	-	chr2_3:61581573-61581592	None:intergenic	20.0%
!!	GATATATGATAGAACATTAT+AGG	+	chr2_3:61581630-61581649	Msa0304270:intron	20.0%
!!!	TATTTTCTGTCTTTAAACTT+TGG	-	chr2_3:61582351-61582370	None:intergenic	20.0%
!!	AAAGTTTAAAGACAGAAAAT+AGG	+	chr2_3:61582350-61582369	Msa0304270:intron	20.0%
!!	CAAGTCATTCAAATTATTAA+AGG	-	chr2_3:61583091-61583110	None:intergenic	20.0%
!!!	TTTAAATTCTGCACAAATTT+TGG	-	chr2_3:61583716-61583735	None:intergenic	20.0%
!!	AGTCATTTATATGAAAAATG+TGG	+	chr2_3:61584672-61584691	Msa0304270:three_prime_UTR	20.0%
!	ATTCATCAAAATCAAGAACA+AGG	-	chr2_3:61580285-61580304	None:intergenic	25.0%
!	ATATCAGAATAAGAGTAGTT+CGG	-	chr2_3:61580618-61580637	None:intergenic	25.0%
!!!	CTTGTAAGATTTTTCAATCA+TGG	+	chr2_3:61580685-61580704	Msa0304270:intron	25.0%
!	TTGAAATAGCAACTATATTG+GGG	-	chr2_3:61580888-61580907	None:intergenic	25.0%
!	ACATTGTACTAAATAGCATA+TGG	+	chr2_3:61580911-61580930	Msa0304270:intron	25.0%
!	GACAAGACTTCATATTATAT+AGG	+	chr2_3:61581000-61581019	Msa0304270:intron	25.0%
!!!	ATTTTTTGTTTGTTTAGGGA+AGG	+	chr2_3:61581058-61581077	Msa0304270:intron	25.0%
!	ACAATATGAAGAATGAAACA+GGG	-	chr2_3:61581404-61581423	None:intergenic	25.0%
!	TTCATTATTTCAGTTGCAAA+TGG	+	chr2_3:61581447-61581466	Msa0304270:intron	25.0%
!	AAAGACAATGAGAATTAGAT+TGG	+	chr2_3:61581788-61581807	Msa0304270:intron	25.0%
!	TTTGGAGGTAAAAAAATGTT+TGG	-	chr2_3:61581836-61581855	None:intergenic	25.0%
!	TTTGAAGAATGATAATTTGG+AGG	-	chr2_3:61581851-61581870	None:intergenic	25.0%
!!	ATATAGGTATTGTAGATTGA+TGG	-	chr2_3:61582030-61582049	None:intergenic	25.0%
!!	TAAAGATGTTTTATGAAGGT+TGG	+	chr2_3:61582375-61582394	Msa0304270:intron	25.0%
!	TTGAAGTTTCCAAAGTTTAA+AGG	+	chr2_3:61582339-61582358	Msa0304270:intron	25.0%
!!	CACATTTTTCCTTTAAACTT+TGG	-	chr2_3:61582483-61582502	None:intergenic	25.0%
!	TGCTTAAAGATGTATTATGA+AGG	+	chr2_3:61582501-61582520	Msa0304270:intron	25.0%
!	GTTATAGTAAGACTTAACAA+CGG	-	chr2_3:61582650-61582669	None:intergenic	25.0%
!	TTAAGTCTTACTATAACAGA+AGG	+	chr2_3:61582653-61582672	Msa0304270:CDS	25.0%
!!!	AACTTGTTTTTTTAATGCAG+AGG	+	chr2_3:61582801-61582820	Msa0304270:intron	25.0%
!	ACATTTAACTCAATTGATCT+TGG	-	chr2_3:61582944-61582963	None:intergenic	25.0%
!!!	TGTCAAAACACAGATTTTTT+AGG	-	chr2_3:61583057-61583076	None:intergenic	25.0%
!	TTATCTGACAATTGATTGTT+TGG	+	chr2_3:61583128-61583147	Msa0304270:intron	25.0%
!	AAGGATCTTATAATTAGGAA+TGG	-	chr2_3:61583196-61583215	None:intergenic	25.0%
!	GTCAAAAGGATCTTATAATT+AGG	-	chr2_3:61583201-61583220	None:intergenic	25.0%
!	GGACTAATAAAATTGTCAAA+AGG	-	chr2_3:61583215-61583234	None:intergenic	25.0%
!	TAAAGTAACTCTTTCTCATA+TGG	+	chr2_3:61583350-61583369	Msa0304270:intron	25.0%
!	AAAGTAACTCTTTCTCATAT+GGG	+	chr2_3:61583351-61583370	Msa0304270:intron	25.0%
!	ATTACGGGAATTAAGAAAAT+TGG	-	chr2_3:61583425-61583444	None:intergenic	25.0%
!!	TTTTCTTAATTCCCGTAATT+TGG	+	chr2_3:61583426-61583445	Msa0304270:intron	25.0%
!!!	TAACTCGTCAATTATTTTAG+AGG	-	chr2_3:61583617-61583636	None:intergenic	25.0%
!!	AGTTAGAGAAATGAATGTTT+TGG	+	chr2_3:61583632-61583651	Msa0304270:intron	25.0%
!	TGTTTGATATAAAGAAACCA+CGG	+	chr2_3:61583798-61583817	Msa0304270:intron	25.0%
!	ATTTAAATAATATGGACGAG+AGG	-	chr2_3:61583888-61583907	None:intergenic	25.0%
!!!	CTGCACTGATTTAAATAATA+TGG	-	chr2_3:61583896-61583915	None:intergenic	25.0%
!	GAAAATGGAATAAGAATAGT+TGG	+	chr2_3:61583951-61583970	Msa0304270:intron	25.0%
!!!	GTGGTGCTAATTAATTTTTT+TGG	+	chr2_3:61584099-61584118	Msa0304270:intron	25.0%
!	TTTGGAAAATTTATTCCTGA+AGG	-	chr2_3:61584200-61584219	None:intergenic	25.0%
!	TTAAATTAAGAGGGAAACTT+TGG	+	chr2_3:61584719-61584738	Msa0304270:three_prime_UTR	25.0%
	AGAATGAGATGAAGAAGTTA+GGG	-	chr2_3:61580162-61580181	None:intergenic	30.0%
!!	GATTTTTCAATCATGGATAG+CGG	+	chr2_3:61580692-61580711	Msa0304270:intron	30.0%
	TTGGGGTGAATAAAAAGATT+TGG	+	chr2_3:61580780-61580799	Msa0304270:intron	30.0%
	TTCCAAAACTATAGTAATGG+AGG	+	chr2_3:61580828-61580847	Msa0304270:intron	30.0%
!	TTCCTCCATTACTATAGTTT+TGG	-	chr2_3:61580833-61580852	None:intergenic	30.0%
!	TATAGTAATGGAGGAAATGT+AGG	+	chr2_3:61580837-61580856	Msa0304270:intron	30.0%
	GAAATGTAGGCAATAGAAAA+TGG	+	chr2_3:61580850-61580869	Msa0304270:intron	30.0%
	TTGTACTAAATAGCATATGG+TGG	+	chr2_3:61580914-61580933	Msa0304270:intron	30.0%
	ACAATTATATATGCAAGCTG+AGG	+	chr2_3:61581151-61581170	Msa0304270:intron	30.0%
	TTGGCAAAACTAATGTTGTT+TGG	+	chr2_3:61581319-61581338	Msa0304270:intron	30.0%
	GACAATATGAAGAATGAAAC+AGG	-	chr2_3:61581405-61581424	None:intergenic	30.0%
!	CAGTGCTTCTGTTAATTTTA+TGG	-	chr2_3:61581919-61581938	None:intergenic	30.0%
	AAATTGCAAAGACATGTAGA+AGG	+	chr2_3:61581972-61581991	Msa0304270:intron	30.0%
	GCTTCTGTATCGAATTATAT+AGG	-	chr2_3:61582046-61582065	None:intergenic	30.0%
	TGCTTACATTGGATATTCTT+TGG	+	chr2_3:61582157-61582176	Msa0304270:intron	30.0%
	TGAGGATATTGGTAAATTGT+TGG	+	chr2_3:61582189-61582208	Msa0304270:intron	30.0%
	AAAACATGTATTAGACAACG+TGG	-	chr2_3:61582577-61582596	None:intergenic	30.0%
	TACTATAACAGAAGGATTGT+TGG	+	chr2_3:61582661-61582680	Msa0304270:CDS	30.0%
	TGGTGATAAGTACTAGAATT+TGG	+	chr2_3:61582757-61582776	Msa0304270:intron	30.0%
	GATCTTATAATTAGGAATGG+AGG	-	chr2_3:61583193-61583212	None:intergenic	30.0%
	CCAAAGAGATCTTATGAAAA+GGG	-	chr2_3:61583236-61583255	None:intergenic	30.0%
	ACCAAAGAGATCTTATGAAA+AGG	-	chr2_3:61583237-61583256	None:intergenic	30.0%
	ATATATGTCACTGTCATAGA+GGG	-	chr2_3:61583518-61583537	None:intergenic	30.0%
	TATATATGTCACTGTCATAG+AGG	-	chr2_3:61583519-61583538	None:intergenic	30.0%
	ACAAAACATAAGCTCAGATA+TGG	-	chr2_3:61583781-61583800	None:intergenic	30.0%
	ATACAGATGACCATTTCAAA+TGG	+	chr2_3:61584021-61584040	Msa0304270:intron	30.0%
	TACAGATGACCATTTCAAAT+GGG	+	chr2_3:61584022-61584041	Msa0304270:intron	30.0%
!!!	TTTTGCATTACAGCTTTAAG+TGG	+	chr2_3:61584080-61584099	Msa0304270:intron	30.0%
	GGAATGTAAAACAATCTCTT+TGG	-	chr2_3:61584218-61584237	None:intergenic	30.0%
	TTCTGTGATTATTTAGACAC+AGG	+	chr2_3:61584307-61584326	Msa0304270:three_prime_UTR	30.0%
	TCTGTGATTATTTAGACACA+GGG	+	chr2_3:61584308-61584327	Msa0304270:three_prime_UTR	30.0%
	ATTAAGCTACAAATCCTGTT+AGG	+	chr2_3:61584341-61584360	Msa0304270:three_prime_UTR	30.0%
	TAGCGTTGCTATCAATTATT+AGG	-	chr2_3:61584384-61584403	None:intergenic	30.0%
	TAGTCCTTAGAATTCAAACA+TGG	+	chr2_3:61584544-61584563	Msa0304270:three_prime_UTR	30.0%
	AGTCCTTAGAATTCAAACAT+GGG	+	chr2_3:61584545-61584564	Msa0304270:three_prime_UTR	30.0%
	AATTCAACACAAACATCAGT+TGG	-	chr2_3:61584602-61584621	None:intergenic	30.0%
	TAAATCAAACCCCCATTTGA+TGG	-	chr2_3:61579902-61579921	None:intergenic	35.0%
!	CAAATGGGGGTTTGATTTAT+TGG	+	chr2_3:61579903-61579922	Msa0304270:five_prime_UTR	35.0%
	GTTTGATTGGAAATGGAAGA+AGG	-	chr2_3:61580068-61580087	None:intergenic	35.0%
!	ATGATGGTGTTTGAGATCTA+CGG	-	chr2_3:61580104-61580123	None:intergenic	35.0%
!	ATGAAGAAGTTAGGGTTTTG+AGG	-	chr2_3:61580154-61580173	None:intergenic	35.0%
	GAGAATGAGATGAAGAAGTT+AGG	-	chr2_3:61580163-61580182	None:intergenic	35.0%
	GGCATAGGAAATTGACAAAA+TGG	-	chr2_3:61580589-61580608	None:intergenic	35.0%
	ATAAGAGTAGTTCGGATTCA+AGG	-	chr2_3:61580610-61580629	None:intergenic	35.0%
	ATAGCAACTATATTGGGGTT+AGG	-	chr2_3:61580883-61580902	None:intergenic	35.0%
	CAACTAATTCACACACACAT+AGG	-	chr2_3:61581221-61581240	None:intergenic	35.0%
!	GTGTGAATTAGTTGTTTCCA+AGG	+	chr2_3:61581227-61581246	Msa0304270:intron	35.0%
!!	TTCCAAGGTTGATTTTGATG+TGG	+	chr2_3:61581242-61581261	Msa0304270:intron	35.0%
!!	TCCAAGGTTGATTTTGATGT+GGG	+	chr2_3:61581243-61581262	Msa0304270:intron	35.0%
	TAAGAAAGACCCAGAGATTA+AGG	+	chr2_3:61581592-61581611	Msa0304270:intron	35.0%
	CAATCAACTCCTTAATCTCT+GGG	-	chr2_3:61581604-61581623	None:intergenic	35.0%
	TCAATCAACTCCTTAATCTC+TGG	-	chr2_3:61581605-61581624	None:intergenic	35.0%
	GATCTTATAATACCATCCCT+AGG	-	chr2_3:61581767-61581786	None:intergenic	35.0%
!	ATTCTTTGGAAGTAAGCATG+AGG	+	chr2_3:61582171-61582190	Msa0304270:intron	35.0%
!	GGCGTAAAGATGTTTTATGA+AGG	+	chr2_3:61582371-61582390	Msa0304270:intron	35.0%
	ACAGATTCGTAAAGAAAGAC+AGG	-	chr2_3:61582717-61582736	None:intergenic	35.0%
	CTTATCACCAGATTGTGAAA+AGG	-	chr2_3:61582747-61582766	None:intergenic	35.0%
	ATTATTGCCCTCAACTTTCA+CGG	+	chr2_3:61583010-61583029	Msa0304270:intron	35.0%
	GTATACATTAAACAGCGACA+AGG	-	chr2_3:61583168-61583187	None:intergenic	35.0%
!	CCCTTTTCATAAGATCTCTT+TGG	+	chr2_3:61583233-61583252	Msa0304270:intron	35.0%
!!!	GAAATGAATGTTTTGGCACT+AGG	+	chr2_3:61583639-61583658	Msa0304270:intron	35.0%
!!!	TCTGCACAAATTTTGGCTAA+AGG	-	chr2_3:61583709-61583728	None:intergenic	35.0%
	CATGCATAAAATTCCTCCAT+GGG	+	chr2_3:61583847-61583866	Msa0304270:intron	35.0%
	ATGCATAAAATTCCTCCATG+GGG	+	chr2_3:61583848-61583867	Msa0304270:intron	35.0%
	TAAATAATATGGACGAGAGG+TGG	-	chr2_3:61583885-61583904	None:intergenic	35.0%
!	AGTAAAGCACCCATTTGAAA+TGG	-	chr2_3:61584034-61584053	None:intergenic	35.0%
!	CATTGCAACGTGATTTTGAA+AGG	-	chr2_3:61584243-61584262	None:intergenic	35.0%
!!!	ATGCTTTGTTTGGTTTTGAC+TGG	+	chr2_3:61584260-61584279	Msa0304270:three_prime_UTR	35.0%
!!!	TCCTGTTAGGTTGTCATTTT+TGG	+	chr2_3:61584354-61584373	Msa0304270:three_prime_UTR	35.0%
	ACCAAAAATGACAACCTAAC+AGG	-	chr2_3:61584358-61584377	None:intergenic	35.0%
!	TTTTAGCACCTATCCACAAA+AGG	+	chr2_3:61584456-61584475	Msa0304270:three_prime_UTR	35.0%
	TTTAGCACCTATCCACAAAA+GGG	+	chr2_3:61584457-61584476	Msa0304270:three_prime_UTR	35.0%
!	TAAACTTGTAGTCCCTTTTG+TGG	-	chr2_3:61584472-61584491	None:intergenic	35.0%
!	TTTCACCTAATGGATCAAAG+CGG	-	chr2_3:61584510-61584529	None:intergenic	35.0%
!!!	TTGACTGCTTTTTCACCTAA+TGG	-	chr2_3:61584520-61584539	None:intergenic	35.0%
	TGTCCCATGTTTGAATTCTA+AGG	-	chr2_3:61584551-61584570	None:intergenic	35.0%
	TGAAAAATGTGGAGACAATC+TGG	+	chr2_3:61584683-61584702	Msa0304270:three_prime_UTR	35.0%
!	GGAAACTTTGGTTCCTTAAT+CGG	+	chr2_3:61584731-61584750	Msa0304270:three_prime_UTR	35.0%
!	TTTGAGATCTACGGTGGTTA+CGG	-	chr2_3:61580095-61580114	None:intergenic	40.0%
	AGATCTCAAACACCATCATG+TGG	+	chr2_3:61580105-61580124	Msa0304270:exon	40.0%
	AGTGAAGATACCTGATTGTG+TGG	-	chr2_3:61580240-61580259	None:intergenic	40.0%
!	GATTTGGTTGTGATTGTAGC+TGG	+	chr2_3:61580308-61580327	Msa0304270:intron	40.0%
	GATGTAGTTCCAATTGCTAC+CGG	+	chr2_3:61580353-61580372	Msa0304270:CDS	40.0%
	GAAAACCACTATAGCGAGTT+AGG	+	chr2_3:61580714-61580733	Msa0304270:intron	40.0%
	AGTATCCTAACTCGCTATAG+TGG	-	chr2_3:61580722-61580741	None:intergenic	40.0%
	GTAGGCAATAGAAAATGGAG+AGG	+	chr2_3:61580855-61580874	Msa0304270:intron	40.0%
!	AGCATATGGTGGAACAATAG+TGG	+	chr2_3:61580925-61580944	Msa0304270:intron	40.0%
!	GTGGAACAATAGTGGTTTGT+TGG	+	chr2_3:61580933-61580952	Msa0304270:intron	40.0%
	CTATAAGCGCTATACCTCTA+TGG	-	chr2_3:61580971-61580990	None:intergenic	40.0%
	TTACTAACCTTCGTGCCAAA+AGG	-	chr2_3:61581114-61581133	None:intergenic	40.0%
	ACCCACATCAAAATCAACCT+TGG	-	chr2_3:61581247-61581266	None:intergenic	40.0%
!!	GTTGATTTTGATGTGGGTTG+CGG	+	chr2_3:61581249-61581268	Msa0304270:intron	40.0%
!	GGCTTGGTTGTTGTTGTATA+CGG	+	chr2_3:61581693-61581712	Msa0304270:intron	40.0%
	GTTATAACTCAGTGAAGGGT+AGG	-	chr2_3:61581734-61581753	None:intergenic	40.0%
!	GGGTGTTATAACTCAGTGAA+GGG	-	chr2_3:61581738-61581757	None:intergenic	40.0%
!	TGGGTGTTATAACTCAGTGA+AGG	-	chr2_3:61581739-61581758	None:intergenic	40.0%
	TTATAACACCCAAGTCTCCT+AGG	+	chr2_3:61581747-61581766	Msa0304270:intron	40.0%
	TATAACACCCAAGTCTCCTA+GGG	+	chr2_3:61581748-61581767	Msa0304270:intron	40.0%
	TCGATACAGAAGCAATCTTC+TGG	+	chr2_3:61582053-61582072	Msa0304270:intron	40.0%
	GGATGCTAAGTTGCTTACAT+TGG	+	chr2_3:61582146-61582165	Msa0304270:intron	40.0%
	GGAAGTAAGCATGAGGATAT+TGG	+	chr2_3:61582178-61582197	Msa0304270:intron	40.0%
!!	GAGGATAGCAACTTGTTAAC+AGG	-	chr2_3:61582216-61582235	None:intergenic	40.0%
	CTCAATTACTTCACCGTACT+CGG	+	chr2_3:61582233-61582252	Msa0304270:intron	40.0%
	TAACCATGGCTGGAAAATCA+AGG	+	chr2_3:61582405-61582424	Msa0304270:intron	40.0%
	AACCATGGCTGGAAAATCAA+GGG	+	chr2_3:61582406-61582425	Msa0304270:intron	40.0%
	TGTCTGCTTTCGCTGTTTAT+AGG	+	chr2_3:61582615-61582634	Msa0304270:intron	40.0%
	TAGTAAGACTTAACAACGGC+AGG	-	chr2_3:61582646-61582665	None:intergenic	40.0%
!	GTGCAAACCTTTTCACAATC+TGG	+	chr2_3:61582737-61582756	Msa0304270:intron	40.0%
!!	AGCATGATGTTGTCTGGTTT+TGG	+	chr2_3:61582827-61582846	Msa0304270:CDS	40.0%
	TGAAAATACTGTGCACACAC+TGG	-	chr2_3:61582880-61582899	None:intergenic	40.0%
!	AGTGTGTGCACAGTATTTTC+AGG	+	chr2_3:61582879-61582898	Msa0304270:CDS	40.0%
	ATGAAGTCCGTGAAAGTTGA+GGG	-	chr2_3:61583020-61583039	None:intergenic	40.0%
	TATGAAGTCCGTGAAAGTTG+AGG	-	chr2_3:61583021-61583040	None:intergenic	40.0%
	TCAACTTTCACGGACTTCAT+AGG	+	chr2_3:61583020-61583039	Msa0304270:intron	40.0%
	ATTCCCGTAATTTGGCCAAA+AGG	+	chr2_3:61583434-61583453	Msa0304270:intron	40.0%
	TTCCCGTAATTTGGCCAAAA+GGG	+	chr2_3:61583435-61583454	Msa0304270:intron	40.0%
	GCATGCATAAAATTCCTCCA+TGG	+	chr2_3:61583846-61583865	Msa0304270:intron	40.0%
	GCTATATATATTCCCCATGG+AGG	-	chr2_3:61583863-61583882	None:intergenic	40.0%
	GTGGCTATATATATTCCCCA+TGG	-	chr2_3:61583866-61583885	None:intergenic	40.0%
	TCTGATCAATGGTGTCCATA+AGG	-	chr2_3:61584165-61584184	None:intergenic	40.0%
	AAACTGCAGCATCTGATCAA+TGG	-	chr2_3:61584176-61584195	None:intergenic	40.0%
!!	GCAACGTGATTTTGAAAGGT+AGG	-	chr2_3:61584239-61584258	None:intergenic	40.0%
!	TCACGTTGCAATGCTTTGTT+TGG	+	chr2_3:61584250-61584269	Msa0304270:three_prime_UTR	40.0%
	GTGATTATTTAGACACAGGG+TGG	+	chr2_3:61584311-61584330	Msa0304270:three_prime_UTR	40.0%
!	TTGTAGTCCCTTTTGTGGAT+AGG	-	chr2_3:61584467-61584486	None:intergenic	40.0%
!!	AAACATCAGTTGGTGGATGA+AGG	-	chr2_3:61584592-61584611	None:intergenic	40.0%
	TCAACACAAACATCAGTTGG+TGG	-	chr2_3:61584599-61584618	None:intergenic	40.0%
	AGTCAAGTCACAACAGTAAC+TGG	-	chr2_3:61584632-61584651	None:intergenic	40.0%
	TACTGTTGTGACTTGACTGT+TGG	+	chr2_3:61584634-61584653	Msa0304270:three_prime_UTR	40.0%
!	TCAAACCCCCATTTGATGGT+GGG	-	chr2_3:61579898-61579917	None:intergenic	45.0%
	ATCAAACCCCCATTTGATGG+TGG	-	chr2_3:61579899-61579918	None:intergenic	45.0%
!	CGCGCTCAAGTTTAACTTCA+AGG	-	chr2_3:61579966-61579985	None:intergenic	45.0%
!	ATGGTGTTTGAGATCTACGG+TGG	-	chr2_3:61580101-61580120	None:intergenic	45.0%
	TCTCAAACACCATCATGTGG+CGG	+	chr2_3:61580108-61580127	Msa0304270:exon	45.0%
!!!	GAAGTTAGGGTTTTGAGGTG+AGG	-	chr2_3:61580149-61580168	None:intergenic	45.0%
	GGTTGTGATTGTAGCTGGTT+CGG	+	chr2_3:61580313-61580332	Msa0304270:intron	45.0%
	TTCGGTGAAGAAGACAGTTG+AGG	+	chr2_3:61580331-61580350	Msa0304270:CDS	45.0%
	AATCTCCTCACGTTCATGAC+CGG	-	chr2_3:61580375-61580394	None:intergenic	45.0%
	GGAGATTCAAGCTGATCTTG+AGG	+	chr2_3:61580388-61580407	Msa0304270:CDS	45.0%
	GTAGTTCGGATTCAAGGCAT+AGG	-	chr2_3:61580604-61580623	None:intergenic	45.0%
	GGAATTCTGCTACACCATAG+AGG	+	chr2_3:61580954-61580973	Msa0304270:intron	45.0%
	CTTCAGGGTGGTCTATTTCA+AGG	-	chr2_3:61581089-61581108	None:intergenic	45.0%
	CTTGAAATAGACCACCCTGA+AGG	+	chr2_3:61581087-61581106	Msa0304270:CDS	45.0%
!!	TTTTGATGTGGGTTGCGGAA+TGG	+	chr2_3:61581254-61581273	Msa0304270:intron	45.0%
	AACAGGGACTCATACCCTTT+AGG	-	chr2_3:61581388-61581407	None:intergenic	45.0%
!	GGTTGGCTACATGGATCAAA+CGG	-	chr2_3:61581657-61581676	None:intergenic	45.0%
!	AAGCCTTATCCCACTAAGCA+GGG	-	chr2_3:61581678-61581697	None:intergenic	45.0%
	AAGGGTAGGTAGGAAGACTA+GGG	-	chr2_3:61581720-61581739	None:intergenic	45.0%
	TAACTCAGTGAAGGGTAGGT+AGG	-	chr2_3:61581730-61581749	None:intergenic	45.0%
	ATACCATCCCTAGGAGACTT+GGG	-	chr2_3:61581758-61581777	None:intergenic	45.0%
	AATACCATCCCTAGGAGACT+TGG	-	chr2_3:61581759-61581778	None:intergenic	45.0%
	TGCAAAGACATGTAGAAGGC+TGG	+	chr2_3:61581976-61581995	Msa0304270:intron	45.0%
	CGAGTACGGTGAAGTAATTG+AGG	-	chr2_3:61582235-61582254	None:intergenic	45.0%
	TCACCTCTTACAACCGAGTA+CGG	-	chr2_3:61582249-61582268	None:intergenic	45.0%
	TAAGAGGTGAAGAGTAACCG+TGG	+	chr2_3:61582259-61582278	Msa0304270:intron	45.0%
	TAACCGTGGCTGGAAAATCA+AGG	+	chr2_3:61582273-61582292	Msa0304270:intron	45.0%
	AACCGTGGCTGGAAAATCAA+GGG	+	chr2_3:61582274-61582293	Msa0304270:intron	45.0%
!!	AGGTTGGTGAAGAGTAACCA+TGG	+	chr2_3:61582391-61582410	Msa0304270:intron	45.0%
	ACCATGGCTGGAAAATCAAG+GGG	+	chr2_3:61582407-61582426	Msa0304270:intron	45.0%
	AGGATGAGCATGATGTTGTC+TGG	+	chr2_3:61582821-61582840	Msa0304270:CDS	45.0%
!!!	TGATGTTGTCTGGTTTTGGC+TGG	+	chr2_3:61582831-61582850	Msa0304270:CDS	45.0%
	TGCACACACTGGACATTCAT+GGG	-	chr2_3:61582869-61582888	None:intergenic	45.0%
!	GACCCTTTTGGCCAAATTAC+GGG	-	chr2_3:61583440-61583459	None:intergenic	45.0%
!	GGACCCTTTTGGCCAAATTA+CGG	-	chr2_3:61583441-61583460	None:intergenic	45.0%
	GCCAAAAGGGTCCTATAGTT+GGG	+	chr2_3:61583448-61583467	Msa0304270:intron	45.0%
	CCAAAAGGGTCCTATAGTTG+GGG	+	chr2_3:61583449-61583468	Msa0304270:intron	45.0%
	CTACATCGATCCCCAACTAT+AGG	-	chr2_3:61583462-61583481	None:intergenic	45.0%
	GCATTCATGAGTGATGACAC+TGG	-	chr2_3:61583752-61583771	None:intergenic	45.0%
	TTCTGCGTTACAGCTTGAAG+TGG	+	chr2_3:61584122-61584141	Msa0304270:intron	45.0%
!	AGATGCTGCAGTTTTCCTTC+AGG	+	chr2_3:61584182-61584201	Msa0304270:three_prime_UTR	45.0%
!!	TTTGACTGGTCTAGACCTTG+CGG	+	chr2_3:61584274-61584293	Msa0304270:three_prime_UTR	45.0%
	TAGGTGCTAAAAGTAGCTGC+AGG	-	chr2_3:61584448-61584467	None:intergenic	45.0%
	CAAGTCACAACAGTAACTGG+TGG	-	chr2_3:61584629-61584648	None:intergenic	45.0%
	GTTGTGACTTGACTGTTGGT+TGG	+	chr2_3:61584638-61584657	Msa0304270:three_prime_UTR	45.0%
	GGGCACTCAAAATCCGATTA+AGG	-	chr2_3:61584747-61584766	None:intergenic	45.0%
!!	TGTATTAAATATATTAAATT+TGG	+	chr2_3:61582125-61582144	Msa0304270:intron	5.0%
!!	CAAACCCCCATTTGATGGTG+GGG	-	chr2_3:61579897-61579916	None:intergenic	50.0%
	CCAACGAATTAGCACGTGAC+GGG	-	chr2_3:61579942-61579961	None:intergenic	50.0%
	CCCAACGAATTAGCACGTGA+CGG	-	chr2_3:61579943-61579962	None:intergenic	50.0%
	CCGTCACGTGCTAATTCGTT+GGG	+	chr2_3:61579940-61579959	Msa0304270:five_prime_UTR	50.0%
	CGGCGTCGTTTGATTGGAAA+TGG	-	chr2_3:61580075-61580094	None:intergenic	50.0%
	TGGTTACGGCGTCGTTTGAT+TGG	-	chr2_3:61580081-61580100	None:intergenic	50.0%
	AACGATTCTCCGCCACATGA+TGG	-	chr2_3:61580120-61580139	None:intergenic	50.0%
	CGTTCATGACCGGTAGCAAT+TGG	-	chr2_3:61580365-61580384	None:intergenic	50.0%
	TCGTGCCAAAAGGACCTTCA+GGG	-	chr2_3:61581104-61581123	None:intergenic	50.0%
	TTCGTGCCAAAAGGACCTTC+AGG	-	chr2_3:61581105-61581124	None:intergenic	50.0%
!	TGAAGGTCCTTTTGGCACGA+AGG	+	chr2_3:61581104-61581123	Msa0304270:CDS	50.0%
!!	TGTGGGTTGCGGAATGGTTT+TGG	+	chr2_3:61581260-61581279	Msa0304270:intron	50.0%
!!	ACTAAGCAGGGTTGGCTACA+TGG	-	chr2_3:61581666-61581685	None:intergenic	50.0%
	ATGTAGCCAACCCTGCTTAG+TGG	+	chr2_3:61581665-61581684	Msa0304270:intron	50.0%
	TGTAGCCAACCCTGCTTAGT+GGG	+	chr2_3:61581666-61581685	Msa0304270:intron	50.0%
	CTTATCCCACTAAGCAGGGT+TGG	-	chr2_3:61581674-61581693	None:intergenic	50.0%
	CAACCCTGCTTAGTGGGATA+AGG	+	chr2_3:61581672-61581691	Msa0304270:intron	50.0%
!	CAAGCCTTATCCCACTAAGC+AGG	-	chr2_3:61581679-61581698	None:intergenic	50.0%
	CTGCTTAGTGGGATAAGGCT+TGG	+	chr2_3:61581677-61581696	Msa0304270:intron	50.0%
	GAAGGGTAGGTAGGAAGACT+AGG	-	chr2_3:61581721-61581740	None:intergenic	50.0%
	TCACCGTACTCGGTTGTAAG+AGG	+	chr2_3:61582243-61582262	Msa0304270:intron	50.0%
	ACCGTGGCTGGAAAATCAAG+GGG	+	chr2_3:61582275-61582294	Msa0304270:intron	50.0%
!	ACCCCTTGATTTTCCAGCCA+CGG	-	chr2_3:61582279-61582298	None:intergenic	50.0%
	TGGTGAAGAGTAACCATGGC+TGG	+	chr2_3:61582395-61582414	Msa0304270:intron	50.0%
!	ACCCCTTGATTTTCCAGCCA+TGG	-	chr2_3:61582279-61582298	None:intergenic	50.0%
	AGAAGGATTGTTGGATGCCC+CGG	+	chr2_3:61582670-61582689	Msa0304270:CDS	50.0%
!!!	TGTTGTCTGGTTTTGGCTGG+AGG	+	chr2_3:61582834-61582853	Msa0304270:CDS	50.0%
	GCACACACTGGACATTCATG+GGG	-	chr2_3:61582868-61582887	None:intergenic	50.0%
	GTGCACACACTGGACATTCA+TGG	-	chr2_3:61582870-61582889	None:intergenic	50.0%
	GGCCAAAAGGGTCCTATAGT+TGG	+	chr2_3:61583447-61583466	Msa0304270:intron	50.0%
!	CCCCAACTATAGGACCCTTT+TGG	-	chr2_3:61583452-61583471	None:intergenic	50.0%
	TGTCAGCATACACTACACCG+TGG	-	chr2_3:61583818-61583837	None:intergenic	50.0%
	TGCGTTACAGCTTGAAGTGG+TGG	+	chr2_3:61584125-61584144	Msa0304270:intron	50.0%
	GCGTTACAGCTTGAAGTGGT+GGG	+	chr2_3:61584126-61584145	Msa0304270:intron	50.0%
	CAGAAACTCTCTGAACCGCA+AGG	-	chr2_3:61584292-61584311	None:intergenic	50.0%
	AGCCTCCGCTTTGATCCATT+AGG	+	chr2_3:61584502-61584521	Msa0304270:three_prime_UTR	50.0%
!	CACCTAATGGATCAAAGCGG+AGG	-	chr2_3:61584507-61584526	None:intergenic	50.0%
	GGACATGTTAATCCCTCCGT+CGG	+	chr2_3:61584566-61584585	Msa0304270:three_prime_UTR	50.0%
	CCCGTCACGTGCTAATTCGT+TGG	+	chr2_3:61579939-61579958	Msa0304270:five_prime_UTR	55.0%
	TACCTGATTGTGTGGCGACG+TGG	-	chr2_3:61580232-61580251	None:intergenic	55.0%
	TGCTACCGGTCATGAACGTG+AGG	+	chr2_3:61580367-61580386	Msa0304270:CDS	55.0%
!	GACCACCCTGAAGGTCCTTT+TGG	+	chr2_3:61581096-61581115	Msa0304270:CDS	55.0%
	TGCCAAAAGGACCTTCAGGG+TGG	-	chr2_3:61581101-61581120	None:intergenic	55.0%
	ACACCCAAGTCTCCTAGGGA+TGG	+	chr2_3:61581752-61581771	Msa0304270:intron	55.0%
	AGGTGAAGAGTAACCGTGGC+TGG	+	chr2_3:61582263-61582282	Msa0304270:intron	55.0%
	ACACTTACCACCCTCACCAC+CGG	-	chr2_3:61582692-61582711	None:intergenic	55.0%
!!!	GTCTGGTTTTGGCTGGAGGA+AGG	+	chr2_3:61582838-61582857	Msa0304270:CDS	55.0%
	TGTCCATAAGGATCACCGCC+AGG	-	chr2_3:61584153-61584172	None:intergenic	55.0%
!!	AGTTGGTGGATGAAGGCCGA+CGG	-	chr2_3:61584585-61584604	None:intergenic	55.0%
	CTGATTGTGTGGCGACGTGG+CGG	-	chr2_3:61580229-61580248	None:intergenic	60.0%
	AGGATTGTTGGATGCCCCGG+TGG	+	chr2_3:61582673-61582692	Msa0304270:CDS	60.0%
	ACTTACCACCCTCACCACCG+GGG	-	chr2_3:61582690-61582709	None:intergenic	60.0%
	CACTTACCACCCTCACCACC+GGG	-	chr2_3:61582691-61582710	None:intergenic	60.0%
!	CAGCTTGAAGTGGTGGGACC+TGG	+	chr2_3:61584132-61584151	Msa0304270:intron	60.0%
!	CTTGAAGTGGTGGGACCTGG+CGG	+	chr2_3:61584135-61584154	Msa0304270:CDS	60.0%
	GGACCTGGCGGTGATCCTTA+TGG	+	chr2_3:61584147-61584166	Msa0304270:CDS	60.0%
	CGCCACGTCGCCACACAATC+AGG	+	chr2_3:61580227-61580246	Msa0304270:CDS	65.0%
	TGTTGGATGCCCCGGTGGTG+AGG	+	chr2_3:61582678-61582697	Msa0304270:CDS	65.0%
	GTTGGATGCCCCGGTGGTGA+GGG	+	chr2_3:61582679-61582698	Msa0304270:CDS	65.0%
	GGTGGATGAAGGCCGACGGA+GGG	-	chr2_3:61584581-61584600	None:intergenic	65.0%
!	TGGTGGATGAAGGCCGACGG+AGG	-	chr2_3:61584582-61584601	None:intergenic	65.0%
	CATCCTCGATCCGCCGTCGC+CGG	+	chr2_3:61580182-61580201	Msa0304270:CDS	70.0%
	GAATCGGAGACCGGCGACGG+CGG	-	chr2_3:61580195-61580214	None:intergenic	70.0%
	GGCGAATCGGAGACCGGCGA+CGG	-	chr2_3:61580198-61580217	None:intergenic	70.0%
!	GTCGCCGGTCTCCGATTCGC+CGG	+	chr2_3:61580197-61580216	Msa0304270:CDS	70.0%
	GAGGCCGGCGAATCGGAGAC+CGG	-	chr2_3:61580204-61580223	None:intergenic	70.0%
	AGACCGGCGACGGCGGATCG+AGG	-	chr2_3:61580188-61580207	None:intergenic	75.0%
	GGCGGCTGAGGCCGGCGAAT+CGG	-	chr2_3:61580211-61580230	None:intergenic	75.0%
	GTGTGGCGACGTGGCGGCTG+AGG	-	chr2_3:61580223-61580242	None:intergenic	75.0%
	GGATGCCCCGGTGGTGAGGG+TGG	+	chr2_3:61582682-61582701	Msa0304270:CDS	75.0%
!	GGCGACGTGGCGGCTGAGGC+CGG	-	chr2_3:61580219-61580238	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	61579892	61584769	61579892	ID=Msa0304270;Name=Msa0304270
chr2_3	mRNA	61579892	61584769	61579892	ID=Msa0304270-mRNA-1;Parent=Msa0304270;Name=Msa0304270-mRNA-1;_AED=0.07;_eAED=0.07;_QI=230\|1\|1\|1\|1\|1\|6\|590\|150
chr2_3	exon	61579892	61580248	61579892	ID=Msa0304270-mRNA-1:exon:20373;Parent=Msa0304270-mRNA-1
chr2_3	exon	61580327	61580409	61580327	ID=Msa0304270-mRNA-1:exon:20374;Parent=Msa0304270-mRNA-1
chr2_3	exon	61581075	61581125	61581075	ID=Msa0304270-mRNA-1:exon:20375;Parent=Msa0304270-mRNA-1
chr2_3	exon	61582637	61582703	61582637	ID=Msa0304270-mRNA-1:exon:20376;Parent=Msa0304270-mRNA-1
chr2_3	exon	61582821	61582900	61582821	ID=Msa0304270-mRNA-1:exon:20377;Parent=Msa0304270-mRNA-1
chr2_3	exon	61584135	61584769	61584135	ID=Msa0304270-mRNA-1:exon:20378;Parent=Msa0304270-mRNA-1
chr2_3	five_prime_UTR	61579892	61580121	61579892	ID=Msa0304270-mRNA-1:five_prime_utr;Parent=Msa0304270-mRNA-1
chr2_3	CDS	61580122	61580248	61580122	ID=Msa0304270-mRNA-1:cds;Parent=Msa0304270-mRNA-1
chr2_3	CDS	61580327	61580409	61580327	ID=Msa0304270-mRNA-1:cds;Parent=Msa0304270-mRNA-1
chr2_3	CDS	61581075	61581125	61581075	ID=Msa0304270-mRNA-1:cds;Parent=Msa0304270-mRNA-1
chr2_3	CDS	61582637	61582703	61582637	ID=Msa0304270-mRNA-1:cds;Parent=Msa0304270-mRNA-1
chr2_3	CDS	61582821	61582900	61582821	ID=Msa0304270-mRNA-1:cds;Parent=Msa0304270-mRNA-1
chr2_3	CDS	61584135	61584179	61584135	ID=Msa0304270-mRNA-1:cds;Parent=Msa0304270-mRNA-1
chr2_3	three_prime_UTR	61584180	61584769	61584180	ID=Msa0304270-mRNA-1:three_prime_utr;Parent=Msa0304270-mRNA-1

Gene Sequence

>Msa0304270

ATGTGGCGGAGAATCGTTTCTTCTCCTCACCTCAAAACCCTAACTTCTTCATCTCATTCTCATCCTCGATCCGCCGTCGCCGGTCTCCGATTCGCCGGCCTCAGCCGCCACGTCGCCACACAATCAGCTGGTTCGGTGAAGAAGACAGTTGAGGATGTAGTTCCAATTGCTACCGGTCATGAACGTGAGGAGATTCAAGCTGATCTTGAGGGAAGGAATATCCTTGAAATAGACCACCCTGAAGGTCCTTTTGGCACGAAGGAAGCACCTGCCGTTGTTAAGTCTTACTATAACAGAAGGATTGTTGGATGCCCCGGTGGTGAGGGTGAGGATGAGCATGATGTTGTCTGGTTTTGGCTGGAGGAAGGCAAGCCCCATGAATGTCCAGTGTGTGCACAGTATTTTCAGCTTGAAGTGGTGGGACCTGGCGGTGATCCTTATGGACACCATTGA

Protein sequence

>Msa0304270

MWRRIVSSPHLKTLTSSSHSHPRSAVAGLRFAGLSRHVATQSAGSVKKTVEDVVPIATGHEREEIQADLEGRNILEIDHPEGPFGTKEAPAVVKSYYNRRIVGCPGGEGEDEHDVVWFWLEEGKPHECPVCAQYFQLEVVGPGGDPYGHH*