AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0884620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0884620 MtrunA17_Chr6g0464971 94.490 363 20 0 1 363 1 363 0.0 720
Msa0884620 MtrunA17_Chr6g0464961 88.981 363 40 0 1 363 23 385 0.0 655
Msa0884620 MtrunA17_Chr6g0465111 62.291 358 135 0 5 362 3 360 5.06e-173 485
Msa0884620 MtrunA17_Chr6g0465121 60.615 358 136 1 5 362 16 368 4.66e-166 468
Msa0884620 MtrunA17_Chr1g0200981 62.429 354 133 0 9 362 6 359 8.04e-164 462
Msa0884620 MtrunA17_Chr6g0477771 53.846 364 163 3 1 361 1 362 1.72e-140 403
Msa0884620 MtrunA17_Chr3g0110001 54.294 361 161 2 5 361 3 363 2.06e-139 400
Msa0884620 MtrunA17_Chr6g0464931 89.091 110 12 0 1 110 1 110 1.05e-57 183
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0884620 AT4G09670.1 59.130 345 141 0 9 353 7 351 5.73e-156 442
Msa0884620 AT1G34200.1 54.830 352 147 2 9 359 7 347 6.35e-136 391
Msa0884620 AT1G66130.1 44.413 358 190 6 5 355 3 358 6.90e-104 310

Find 111 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATGAGCCATATTATTTC+TGG 0.141659 6_1:-38312436 None:intergenic
ACCCCTGCTTCATTGTATTT+TGG 0.174819 6_1:-38314359 None:intergenic
ATTCTAAAGCTGACAATAAA+TGG 0.176493 6_1:+38314693 Msa0884620:CDS
CCAAGGATTCCAAATCTTAT+TGG 0.177750 6_1:-38312468 None:intergenic
TGTTTAATATGTAAAGATAT+TGG 0.186002 6_1:+38315059 Msa0884620:three_prime_UTR
TTTAATAAAAGTACATATTA+AGG 0.217593 6_1:-38315144 None:intergenic
AACGGGTCCCATGTTTAAAA+AGG 0.239516 6_1:+38314936 Msa0884620:three_prime_UTR
CATATTCATATCTGATAAAA+AGG 0.244809 6_1:-38314444 None:intergenic
TGGAGAACTTGACAAGATTT+TGG 0.253525 6_1:+38312776 Msa0884620:CDS
ACTATAACCTCACTTGGTTT+TGG 0.256553 6_1:-38314596 None:intergenic
ATATCTGATGCTAATCTCTT+TGG 0.262383 6_1:+38312882 Msa0884620:CDS
GTTTAATATGTAAAGATATT+GGG 0.295697 6_1:+38315060 Msa0884620:three_prime_UTR
CTTGTGAAGCTTCTTTGATA+TGG 0.295852 6_1:+38314390 Msa0884620:CDS
AAGTATATAACAACTAAATT+TGG 0.305533 6_1:-38312311 None:intergenic
TCTCTCTTCCAGAAATAATA+TGG 0.307767 6_1:+38312428 Msa0884620:exon
ATTATTTCTGGAAGAGAGAT+TGG 0.321772 6_1:-38312424 None:intergenic
CCAATAAGATTTGGAATCCT+TGG 0.324504 6_1:+38312468 Msa0884620:CDS
CTGGTTGGTGGGATTAAGTA+TGG 0.329892 6_1:+38314668 Msa0884620:CDS
AATATGGATATAACAGCAAT+AGG 0.330557 6_1:+38314461 Msa0884620:CDS
GGTTAAGGAATTTGCTCATC+TGG 0.331077 6_1:+38314649 Msa0884620:CDS
ATCTAATGGATTGCAGTATA+TGG 0.331709 6_1:+38312809 Msa0884620:CDS
GAAACAAAACCAATAAGATT+TGG 0.345157 6_1:+38312459 Msa0884620:CDS
CCCCGTTAAGTATCCTCTAT+TGG 0.356343 6_1:+38314846 Msa0884620:three_prime_UTR
CTCTTTCTTAAGTAATTAAC+GGG 0.358325 6_1:+38314919 Msa0884620:three_prime_UTR
TTGGAAGCTTGTGAATCTAA+TGG 0.364162 6_1:+38312795 Msa0884620:CDS
CATTATTAGTGTATTGTCAT+TGG 0.369644 6_1:+38315110 Msa0884620:three_prime_UTR
TGCATGGGAAGGGAAATTAT+TGG 0.373095 6_1:-38312601 None:intergenic
ATGTCCTTTGCTAAGAAATC+TGG 0.373101 6_1:-38314215 None:intergenic
TCTCCAACATTTAAAGCAAC+TGG 0.374707 6_1:-38312759 None:intergenic
TATTCCACAAGAAGCACTTA+TGG 0.376037 6_1:+38314628 Msa0884620:CDS
AACTCAACCCTCTACGCAAT+TGG 0.384123 6_1:+38312540 Msa0884620:CDS
ATAGTTGCTCCTTCCTTAAC+GGG 0.385479 6_1:-38314887 None:intergenic
CAACTAGCTTACACCTTCAC+TGG 0.388078 6_1:+38312691 Msa0884620:CDS
AAAGCCTCATTCTCTTCATT+AGG 0.391917 6_1:-38314521 None:intergenic
ACAAGAAGCACTTATGGTTA+AGG 0.393073 6_1:+38314634 Msa0884620:CDS
TCTCTTTCTTAAGTAATTAA+CGG 0.395989 6_1:+38314918 Msa0884620:three_prime_UTR
GTGAAAGCTTCAATTGATAA+TGG 0.396592 6_1:+38314755 Msa0884620:CDS
ATTGCGTAGAGGGTTGAGTT+TGG 0.401110 6_1:-38312537 None:intergenic
TATAGTTGCTCCTTCCTTAA+CGG 0.404751 6_1:-38314888 None:intergenic
TCCAATAGAGGATACTTAAC+GGG 0.413630 6_1:-38314847 None:intergenic
GAGGGTTGAGTTTGGAGAGA+GGG 0.413807 6_1:-38312529 None:intergenic
AAACCAGTTGCTTTAAATGT+TGG 0.419578 6_1:+38312756 Msa0884620:CDS
GAGATGCTCTAATACTCAAT+AGG 0.424576 6_1:-38314791 None:intergenic
ATAACAGCAATAGGGGCAAA+AGG 0.425672 6_1:+38314470 Msa0884620:CDS
CTCTAATACTCAATAGGAAC+AGG 0.435951 6_1:-38314785 None:intergenic
TCTCTTTGGTTCCCTTCAAT+CGG 0.438224 6_1:+38312896 Msa0884620:CDS
TGAGTTTGGAGAGAGGGTTA+TGG 0.439944 6_1:-38312523 None:intergenic
ACTTGGTCCCACTTACATAA+AGG 0.441745 6_1:+38314975 Msa0884620:three_prime_UTR
ATATGGATATAACAGCAATA+GGG 0.448214 6_1:+38314462 Msa0884620:CDS
CTCCAATAGAGGATACTTAA+CGG 0.448905 6_1:-38314848 None:intergenic
ATAAATGGGCAATTATTAGT+AGG 0.455056 6_1:+38314708 Msa0884620:CDS
TTGTGAAGCTTCTTTGATAT+GGG 0.455911 6_1:+38314391 Msa0884620:CDS
TTATTACTCCTTTATGTAAG+TGG 0.460226 6_1:-38314983 None:intergenic
GTTCCTAGTGCATCAAGATC+TGG 0.470430 6_1:-38314248 None:intergenic
AAACCAAAATACAATGAAGC+AGG 0.471830 6_1:+38314356 Msa0884620:CDS
TTCTAAAGCTGACAATAAAT+GGG 0.476445 6_1:+38314694 Msa0884620:CDS
ATGTAGACGGCATCGACGTC+CGG 0.478389 6_1:-38312660 None:intergenic
ACTTGAGTGTGGTTATACTA+AGG 0.491210 6_1:-38312393 None:intergenic
AGTATTAGAGCATCTCCAAT+GGG 0.494063 6_1:+38314798 Msa0884620:exon
GTGTGGTTATACTAAGGATT+GGG 0.494254 6_1:-38312387 None:intergenic
GGATGAATCATACCGATTGA+AGG 0.499775 6_1:-38312908 None:intergenic
AGAGGGTTGAGTTTGGAGAG+AGG 0.500740 6_1:-38312530 None:intergenic
AAGGAATTTGCTCATCTGGT+TGG 0.501930 6_1:+38314653 Msa0884620:CDS
AATTTGCTCATCTGGTTGGT+GGG 0.506597 6_1:+38314657 Msa0884620:CDS
TATTACTCCTTTATGTAAGT+GGG 0.513293 6_1:-38314982 None:intergenic
CTTGATGCACTAGGAACACT+AGG 0.518180 6_1:+38314254 Msa0884620:CDS
GAATTTGCTCATCTGGTTGG+TGG 0.520943 6_1:+38314656 Msa0884620:CDS
TTGTTGAACTTTCTACTGGT+TGG 0.522425 6_1:+38314570 Msa0884620:CDS
GTAATAAAATGCGTAAAACT+TGG 0.525237 6_1:+38314958 Msa0884620:three_prime_UTR
CAGTGAAGGTGTAAGCTAGT+TGG 0.530151 6_1:-38312690 None:intergenic
GGAGTGGTCCTTGTTAAGTA+AGG 0.533286 6_1:-38314825 None:intergenic
GCTAAGAAATCTGGACTCAC+CGG 0.540515 6_1:-38314206 None:intergenic
ACGGCTTCCAATTGCGTAGA+GGG 0.541391 6_1:-38312547 None:intergenic
ATTACTCATTTAGACAAGTA+CGG 0.545208 6_1:-38315022 None:intergenic
AGTGTGGTTATACTAAGGAT+TGG 0.545209 6_1:-38312388 None:intergenic
AGAGGATACTTAACGGGGAG+TGG 0.547127 6_1:-38314841 None:intergenic
TTGTTGCTTTCTCTAATGAA+CGG 0.550206 6_1:-38312566 None:intergenic
CGTTAAGTATCCTCTATTGG+AGG 0.550849 6_1:+38314849 Msa0884620:three_prime_UTR
TAACAGCAATAGGGGCAAAA+GGG 0.550871 6_1:+38314471 Msa0884620:CDS
TTAACCATAAGTGCTTCTTG+TGG 0.562009 6_1:-38314632 None:intergenic
GATGAATCATACCGATTGAA+GGG 0.566141 6_1:-38312907 None:intergenic
TATACCTAATGAAGAGAATG+AGG 0.567350 6_1:+38314517 Msa0884620:CDS
AACCAAAATACAATGAAGCA+GGG 0.567968 6_1:+38314357 Msa0884620:CDS
GACGTCCGGATCATCTAAAA+CGG 0.571998 6_1:-38312646 None:intergenic
CTAAGAAATCTGGACTCACC+GGG 0.573636 6_1:-38314205 None:intergenic
CAGCAAGAACTGCCCAGTGA+AGG 0.578576 6_1:-38312704 None:intergenic
TGGAAAAGTAAACTTGAGTG+TGG 0.578718 6_1:-38312404 None:intergenic
TATGGATATAACAGCAATAG+GGG 0.579131 6_1:+38314463 Msa0884620:CDS
GAGTATTAGAGCATCTCCAA+TGG 0.579422 6_1:+38314797 Msa0884620:exon
AGGTGTAAGCTAGTTGGAAG+TGG 0.584079 6_1:-38312684 None:intergenic
AAATGAGTTCCCGTTAAGGA+AGG 0.592471 6_1:+38314878 Msa0884620:three_prime_UTR
CTAGGAACACTAGGTGACAC+TGG 0.593492 6_1:+38314263 Msa0884620:CDS
GAGTCCAGATTTCTTAGCAA+AGG 0.596624 6_1:+38314211 Msa0884620:CDS
AGTTGGAAGTGGCATGTAGA+CGG 0.596812 6_1:-38312673 None:intergenic
TGGTTGGTGGGATTAAGTAT+GGG 0.605617 6_1:+38314669 Msa0884620:CDS
AAGTATCCTCTATTGGAGGA+AGG 0.607049 6_1:+38314853 Msa0884620:three_prime_UTR
GAACACTAGGTGACACTGGT+TGG 0.608823 6_1:+38314267 Msa0884620:CDS
AACTAGCTTACACCTTCACT+GGG 0.612275 6_1:+38312692 Msa0884620:CDS
AAGCCAGATCTTGATGCACT+AGG 0.620052 6_1:+38314245 Msa0884620:CDS
TATTAGTAGGAAAACTCAAG+TGG 0.621897 6_1:+38314721 Msa0884620:CDS
AAGGATTGGGTGGCTACTAG+AGG 0.627512 6_1:-38312374 None:intergenic
TAGCTATCTCTGCACAACCA+AGG 0.627873 6_1:-38312485 None:intergenic
AACGGCTTCCAATTGCGTAG+AGG 0.629775 6_1:-38312548 None:intergenic
TGGTTATACTAAGGATTGGG+TGG 0.635340 6_1:-38312384 None:intergenic
ACCAAAATACAATGAAGCAG+GGG 0.645787 6_1:+38314358 Msa0884620:CDS
TATTAGAGCATCTCCAATGG+GGG 0.655302 6_1:+38314800 Msa0884620:exon
TTGGAATCCAAAACCAAGTG+AGG 0.661767 6_1:+38314589 Msa0884620:CDS
CCAATAGAGGATACTTAACG+GGG 0.684716 6_1:-38314846 None:intergenic
ATTAGAGCATCTCCAATGGG+GGG 0.701573 6_1:+38314801 Msa0884620:exon
GTATTAGAGCATCTCCAATG+GGG 0.721305 6_1:+38314799 Msa0884620:exon
AGTATATGGATTCTACTATG+TGG 0.724513 6_1:+38312823 Msa0884620:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+AGG + chr6_1:38313847-38313866 Msa0884620:intron 0.0%
!!! AAAAGTATTTTATATTTTCT+TGG - chr6_1:38313779-38313798 None:intergenic 10.0%
!! ATATATATATATATTTCTCA+TGG - chr6_1:38313841-38313860 None:intergenic 10.0%
!!! CATATTTAAATATTTTTCAT+TGG + chr6_1:38315084-38315103 Msa0884620:three_prime_UTR 10.0%
!! AAGTATATAACAACTAAATT+TGG - chr6_1:38312314-38312333 None:intergenic 15.0%
!!! AAAACTAAAAAAGAGTTTTT+GGG - chr6_1:38313234-38313253 None:intergenic 15.0%
!!! AAAAACTAAAAAAGAGTTTT+TGG - chr6_1:38313235-38313254 None:intergenic 15.0%
!!! GTTTTGAATTTTTTAGTTTT+AGG + chr6_1:38313317-38313336 Msa0884620:intron 15.0%
!! AAAAAACTAAAACATGTAAA+AGG + chr6_1:38313344-38313363 Msa0884620:intron 15.0%
!! CCTCAAAAATAAAATAAAAA+CGG + chr6_1:38313576-38313595 Msa0884620:intron 15.0%
!! AACATAATTATATCTATGTA+TGG - chr6_1:38313617-38313636 None:intergenic 15.0%
!!! TGTAAAGATAATCATTTTAT+AGG + chr6_1:38313658-38313677 Msa0884620:intron 15.0%
!! CAATAAAACACAAAATTATA+TGG + chr6_1:38313749-38313768 Msa0884620:intron 15.0%
!! CGTTAATTATATTTAATTAC+AGG + chr6_1:38314163-38314182 Msa0884620:intron 15.0%
!!! TTTATTACCTTTTTAAACAT+GGG - chr6_1:38314946-38314965 None:intergenic 15.0%
!!! TTTTATTACCTTTTTAAACA+TGG - chr6_1:38314947-38314966 None:intergenic 15.0%
!! TGTTTAATATGTAAAGATAT+TGG + chr6_1:38315059-38315078 Msa0884620:three_prime_UTR 15.0%
!! GTTTAATATGTAAAGATATT+GGG + chr6_1:38315060-38315079 Msa0884620:three_prime_UTR 15.0%
!! CAAAAATTGAAACATAAGAA+AGG - chr6_1:38312936-38312955 None:intergenic 20.0%
!!! TTCTTCTTATGTTTTTTTAG+AGG + chr6_1:38312996-38313015 Msa0884620:intron 20.0%
!!! GAAATCATTTTTTTTACAAG+AGG + chr6_1:38313061-38313080 Msa0884620:intron 20.0%
!!! AATCATTGTTGTAACTTTTA+AGG - chr6_1:38313100-38313119 None:intergenic 20.0%
!! TTAAAAGTTACAACAATGAT+TGG + chr6_1:38313099-38313118 Msa0884620:intron 20.0%
!! TAAAAGTTACAACAATGATT+GGG + chr6_1:38313100-38313119 Msa0884620:intron 20.0%
!! ATTCAAAACAGTTTACAAAA+CGG - chr6_1:38313306-38313325 None:intergenic 20.0%
!! AAACAAAAACTATTTCAAAC+AGG + chr6_1:38313408-38313427 Msa0884620:intron 20.0%
!!! CCGTTTTTATTTTATTTTTG+AGG - chr6_1:38313579-38313598 None:intergenic 20.0%
!!! TATATATATATAGGCGTTTT+TGG + chr6_1:38313898-38313917 Msa0884620:intron 20.0%
!! TAAACAACATCATTAACAAT+CGG - chr6_1:38313927-38313946 None:intergenic 20.0%
!!! ATTGTATTTTGGTTTATGTA+AGG - chr6_1:38314351-38314370 None:intergenic 20.0%
!! CATATTCATATCTGATAAAA+AGG - chr6_1:38314447-38314466 None:intergenic 20.0%
!!! CTTTTTATCAGATATGAATA+TGG + chr6_1:38314445-38314464 Msa0884620:CDS 20.0%
!! TCTCTTTCTTAAGTAATTAA+CGG + chr6_1:38314918-38314937 Msa0884620:three_prime_UTR 20.0%
!!! AAATACTCTTTGTTTTGAGA+AGG - chr6_1:38312346-38312365 None:intergenic 25.0%
! GAAACAAAACCAATAAGATT+TGG + chr6_1:38312459-38312478 Msa0884620:CDS 25.0%
!!! TGAAACATTTTTTCAGTTCT+AGG - chr6_1:38312858-38312877 None:intergenic 25.0%
! AATTGAAACATAAGAAAGGA+TGG - chr6_1:38312932-38312951 None:intergenic 25.0%
! TTTATTGTCAAGAAACAATC+TGG - chr6_1:38313736-38313755 None:intergenic 25.0%
!!! TCAATTAGAAAAGTACTAAC+AGG + chr6_1:38314133-38314152 Msa0884620:intron 25.0%
!! ATTGCATTAGAGCAATTTTA+TGG + chr6_1:38314291-38314310 Msa0884620:CDS 25.0%
!! TTGCATTAGAGCAATTTTAT+GGG + chr6_1:38314292-38314311 Msa0884620:CDS 25.0%
! AATATGGATATAACAGCAAT+AGG + chr6_1:38314461-38314480 Msa0884620:CDS 25.0%
! ATATGGATATAACAGCAATA+GGG + chr6_1:38314462-38314481 Msa0884620:CDS 25.0%
!! TATTTTGTTGAACTTTCTAC+TGG + chr6_1:38314566-38314585 Msa0884620:CDS 25.0%
!!! TTTTTAACTATAACCTCACT+TGG - chr6_1:38314605-38314624 None:intergenic 25.0%
!!! ATTCTAAAGCTGACAATAAA+TGG + chr6_1:38314693-38314712 Msa0884620:CDS 25.0%
!!! TTCTAAAGCTGACAATAAAT+GGG + chr6_1:38314694-38314713 Msa0884620:CDS 25.0%
! ATAAATGGGCAATTATTAGT+AGG + chr6_1:38314708-38314727 Msa0884620:CDS 25.0%
! CTCTTTCTTAAGTAATTAAC+GGG + chr6_1:38314919-38314938 Msa0884620:three_prime_UTR 25.0%
! GTAATAAAATGCGTAAAACT+TGG + chr6_1:38314958-38314977 Msa0884620:three_prime_UTR 25.0%
! TATTACTCCTTTATGTAAGT+GGG - chr6_1:38314985-38315004 None:intergenic 25.0%
! TTATTACTCCTTTATGTAAG+TGG - chr6_1:38314986-38315005 None:intergenic 25.0%
! ATTACTCATTTAGACAAGTA+CGG - chr6_1:38315025-38315044 None:intergenic 25.0%
! CATTATTAGTGTATTGTCAT+TGG + chr6_1:38315110-38315129 Msa0884620:three_prime_UTR 25.0%
!! ATTATTTCTGGAAGAGAGAT+TGG - chr6_1:38312427-38312446 None:intergenic 30.0%
TCTCTCTTCCAGAAATAATA+TGG + chr6_1:38312428-38312447 Msa0884620:exon 30.0%
TTCATGAGCCATATTATTTC+TGG - chr6_1:38312439-38312458 None:intergenic 30.0%
!! TTGTTGCTTTCTCTAATGAA+CGG - chr6_1:38312569-38312588 None:intergenic 30.0%
!! TGAACCGTATATTTTTGCAT+GGG - chr6_1:38312619-38312638 None:intergenic 30.0%
!! ATGAACCGTATATTTTTGCA+TGG - chr6_1:38312620-38312639 None:intergenic 30.0%
! AAACCAGTTGCTTTAAATGT+TGG + chr6_1:38312756-38312775 Msa0884620:CDS 30.0%
!! ATCTAATGGATTGCAGTATA+TGG + chr6_1:38312809-38312828 Msa0884620:CDS 30.0%
AGTATATGGATTCTACTATG+TGG + chr6_1:38312823-38312842 Msa0884620:CDS 30.0%
ATATCTGATGCTAATCTCTT+TGG + chr6_1:38312882-38312901 Msa0884620:CDS 30.0%
! ACTTTGATCCATTGACTATT+TGG + chr6_1:38313980-38313999 Msa0884620:intron 30.0%
AAACCAAAATACAATGAAGC+AGG + chr6_1:38314356-38314375 Msa0884620:CDS 30.0%
AACCAAAATACAATGAAGCA+GGG + chr6_1:38314357-38314376 Msa0884620:CDS 30.0%
TTGTGAAGCTTCTTTGATAT+GGG + chr6_1:38314391-38314410 Msa0884620:CDS 30.0%
TATGGATATAACAGCAATAG+GGG + chr6_1:38314463-38314482 Msa0884620:CDS 30.0%
TATACCTAATGAAGAGAATG+AGG + chr6_1:38314517-38314536 Msa0884620:CDS 30.0%
TATTAGTAGGAAAACTCAAG+TGG + chr6_1:38314721-38314740 Msa0884620:CDS 30.0%
GTGAAAGCTTCAATTGATAA+TGG + chr6_1:38314755-38314774 Msa0884620:CDS 30.0%
!!! CTTTGTTTTGAGAAGGAGAA+AGG - chr6_1:38312339-38312358 None:intergenic 35.0%
GTGTGGTTATACTAAGGATT+GGG - chr6_1:38312390-38312409 None:intergenic 35.0%
AGTGTGGTTATACTAAGGAT+TGG - chr6_1:38312391-38312410 None:intergenic 35.0%
ACTTGAGTGTGGTTATACTA+AGG - chr6_1:38312396-38312415 None:intergenic 35.0%
TGGAAAAGTAAACTTGAGTG+TGG - chr6_1:38312407-38312426 None:intergenic 35.0%
CCAAGGATTCCAAATCTTAT+TGG - chr6_1:38312471-38312490 None:intergenic 35.0%
! CCAATAAGATTTGGAATCCT+TGG + chr6_1:38312468-38312487 Msa0884620:CDS 35.0%
!! CGTATATTTTTGCATGGGAA+GGG - chr6_1:38312614-38312633 None:intergenic 35.0%
CCTTCCCATGCAAAAATATA+CGG + chr6_1:38312612-38312631 Msa0884620:CDS 35.0%
! TCTCCAACATTTAAAGCAAC+TGG - chr6_1:38312762-38312781 None:intergenic 35.0%
! TGGAGAACTTGACAAGATTT+TGG + chr6_1:38312776-38312795 Msa0884620:CDS 35.0%
TTGGAAGCTTGTGAATCTAA+TGG + chr6_1:38312795-38312814 Msa0884620:CDS 35.0%
! GATGAATCATACCGATTGAA+GGG - chr6_1:38312910-38312929 None:intergenic 35.0%
AGGATAACTATTGTGAGAAG+AGG + chr6_1:38313364-38313383 Msa0884620:intron 35.0%
! TTGTGTCATGCTACGTTTTT+GGG - chr6_1:38313951-38313970 None:intergenic 35.0%
! TTTGTGTCATGCTACGTTTT+TGG - chr6_1:38313952-38313971 None:intergenic 35.0%
ACTATGAGCCAAATAGTCAA+TGG - chr6_1:38313991-38314010 None:intergenic 35.0%
TTGGCTCATAGTTTGATTGT+TGG + chr6_1:38313999-38314018 Msa0884620:intron 35.0%
!! TTTGTTTTCGCAATTGTGCT+TGG + chr6_1:38314049-38314068 Msa0884620:intron 35.0%
TTGTGCTTGGAGAATATCAT+TGG + chr6_1:38314062-38314081 Msa0884620:intron 35.0%
! ACATGCTACATTTTCTTACC+CGG + chr6_1:38314187-38314206 Msa0884620:CDS 35.0%
ATGTCCTTTGCTAAGAAATC+TGG - chr6_1:38314218-38314237 None:intergenic 35.0%
!!! TGTAAGGCTTTTGCTGTTTT+GGG - chr6_1:38314335-38314354 None:intergenic 35.0%
!!! ATGTAAGGCTTTTGCTGTTT+TGG - chr6_1:38314336-38314355 None:intergenic 35.0%
ACCAAAATACAATGAAGCAG+GGG + chr6_1:38314358-38314377 Msa0884620:CDS 35.0%
CTTGTGAAGCTTCTTTGATA+TGG + chr6_1:38314390-38314409 Msa0884620:CDS 35.0%
AAAGCCTCATTCTCTTCATT+AGG - chr6_1:38314524-38314543 None:intergenic 35.0%
TTGTTGAACTTTCTACTGGT+TGG + chr6_1:38314570-38314589 Msa0884620:CDS 35.0%
! ACTATAACCTCACTTGGTTT+TGG - chr6_1:38314599-38314618 None:intergenic 35.0%
!! TATTCCACAAGAAGCACTTA+TGG + chr6_1:38314628-38314647 Msa0884620:CDS 35.0%
TTAACCATAAGTGCTTCTTG+TGG - chr6_1:38314635-38314654 None:intergenic 35.0%
!! ACAAGAAGCACTTATGGTTA+AGG + chr6_1:38314634-38314653 Msa0884620:CDS 35.0%
CTCTAATACTCAATAGGAAC+AGG - chr6_1:38314788-38314807 None:intergenic 35.0%
GAGATGCTCTAATACTCAAT+AGG - chr6_1:38314794-38314813 None:intergenic 35.0%
! AGTATTAGAGCATCTCCAAT+GGG + chr6_1:38314798-38314817 Msa0884620:exon 35.0%
TCCAATAGAGGATACTTAAC+GGG - chr6_1:38314850-38314869 None:intergenic 35.0%
CTCCAATAGAGGATACTTAA+CGG - chr6_1:38314851-38314870 None:intergenic 35.0%
! ATTTTTCCTTCCTCCAATAG+AGG - chr6_1:38314862-38314881 None:intergenic 35.0%
TATAGTTGCTCCTTCCTTAA+CGG - chr6_1:38314891-38314910 None:intergenic 35.0%
TGGTTATACTAAGGATTGGG+TGG - chr6_1:38312387-38312406 None:intergenic 40.0%
TGCATGGGAAGGGAAATTAT+TGG - chr6_1:38312604-38312623 None:intergenic 40.0%
!! CCGTATATTTTTGCATGGGA+AGG - chr6_1:38312615-38312634 None:intergenic 40.0%
! AACTAGCTTACACCTTCACT+GGG + chr6_1:38312692-38312711 Msa0884620:CDS 40.0%
! TCTCTTTGGTTCCCTTCAAT+CGG + chr6_1:38312896-38312915 Msa0884620:CDS 40.0%
! GGATGAATCATACCGATTGA+AGG - chr6_1:38312911-38312930 None:intergenic 40.0%
GAGTCCAGATTTCTTAGCAA+AGG + chr6_1:38314211-38314230 Msa0884620:CDS 40.0%
! ACCCCTGCTTCATTGTATTT+TGG - chr6_1:38314362-38314381 None:intergenic 40.0%
ATAACAGCAATAGGGGCAAA+AGG + chr6_1:38314470-38314489 Msa0884620:CDS 40.0%
TAACAGCAATAGGGGCAAAA+GGG + chr6_1:38314471-38314490 Msa0884620:CDS 40.0%
TTGGAATCCAAAACCAAGTG+AGG + chr6_1:38314589-38314608 Msa0884620:CDS 40.0%
! GGTTAAGGAATTTGCTCATC+TGG + chr6_1:38314649-38314668 Msa0884620:CDS 40.0%
! AAGGAATTTGCTCATCTGGT+TGG + chr6_1:38314653-38314672 Msa0884620:CDS 40.0%
! AATTTGCTCATCTGGTTGGT+GGG + chr6_1:38314657-38314676 Msa0884620:CDS 40.0%
!! TGGTTGGTGGGATTAAGTAT+GGG + chr6_1:38314669-38314688 Msa0884620:CDS 40.0%
! GAGTATTAGAGCATCTCCAA+TGG + chr6_1:38314797-38314816 Msa0884620:exon 40.0%
! GTATTAGAGCATCTCCAATG+GGG + chr6_1:38314799-38314818 Msa0884620:exon 40.0%
! TATTAGAGCATCTCCAATGG+GGG + chr6_1:38314800-38314819 Msa0884620:exon 40.0%
CCAATAGAGGATACTTAACG+GGG - chr6_1:38314849-38314868 None:intergenic 40.0%
CGTTAAGTATCCTCTATTGG+AGG + chr6_1:38314849-38314868 Msa0884620:three_prime_UTR 40.0%
AAGTATCCTCTATTGGAGGA+AGG + chr6_1:38314853-38314872 Msa0884620:three_prime_UTR 40.0%
GGAAAAATGAGTTCCCGTTA+AGG + chr6_1:38314874-38314893 Msa0884620:three_prime_UTR 40.0%
AAATGAGTTCCCGTTAAGGA+AGG + chr6_1:38314878-38314897 Msa0884620:three_prime_UTR 40.0%
ATAGTTGCTCCTTCCTTAAC+GGG - chr6_1:38314890-38314909 None:intergenic 40.0%
AACGGGTCCCATGTTTAAAA+AGG + chr6_1:38314936-38314955 Msa0884620:three_prime_UTR 40.0%
ACTTGGTCCCACTTACATAA+AGG + chr6_1:38314975-38314994 Msa0884620:three_prime_UTR 40.0%
TAGCTATCTCTGCACAACCA+AGG - chr6_1:38312488-38312507 None:intergenic 45.0%
TGAGTTTGGAGAGAGGGTTA+TGG - chr6_1:38312526-38312545 None:intergenic 45.0%
ATTGCGTAGAGGGTTGAGTT+TGG - chr6_1:38312540-38312559 None:intergenic 45.0%
AACTCAACCCTCTACGCAAT+TGG + chr6_1:38312540-38312559 Msa0884620:CDS 45.0%
GACGTCCGGATCATCTAAAA+CGG - chr6_1:38312649-38312668 None:intergenic 45.0%
AGTTGGAAGTGGCATGTAGA+CGG - chr6_1:38312676-38312695 None:intergenic 45.0%
AGGTGTAAGCTAGTTGGAAG+TGG - chr6_1:38312687-38312706 None:intergenic 45.0%
! CAGTGAAGGTGTAAGCTAGT+TGG - chr6_1:38312693-38312712 None:intergenic 45.0%
! CAACTAGCTTACACCTTCAC+TGG + chr6_1:38312691-38312710 Msa0884620:CDS 45.0%
CAGAGACAGAGACGAAGTAT+AGG + chr6_1:38313537-38313556 Msa0884620:intron 45.0%
! TTGTTGGTCTAGCTACCTCA+AGG + chr6_1:38314015-38314034 Msa0884620:intron 45.0%
! TGTTGGTCTAGCTACCTCAA+GGG + chr6_1:38314016-38314035 Msa0884620:intron 45.0%
CTAAGAAATCTGGACTCACC+GGG - chr6_1:38314208-38314227 None:intergenic 45.0%
GCTAAGAAATCTGGACTCAC+CGG - chr6_1:38314209-38314228 None:intergenic 45.0%
AAGCCAGATCTTGATGCACT+AGG + chr6_1:38314245-38314264 Msa0884620:CDS 45.0%
GTTCCTAGTGCATCAAGATC+TGG - chr6_1:38314251-38314270 None:intergenic 45.0%
CTTGATGCACTAGGAACACT+AGG + chr6_1:38314254-38314273 Msa0884620:CDS 45.0%
! GAATTTGCTCATCTGGTTGG+TGG + chr6_1:38314656-38314675 Msa0884620:CDS 45.0%
!! CTGGTTGGTGGGATTAAGTA+TGG + chr6_1:38314668-38314687 Msa0884620:CDS 45.0%
! ATTAGAGCATCTCCAATGGG+GGG + chr6_1:38314801-38314820 Msa0884620:exon 45.0%
GGAGTGGTCCTTGTTAAGTA+AGG - chr6_1:38314828-38314847 None:intergenic 45.0%
CCCCGTTAAGTATCCTCTAT+TGG + chr6_1:38314846-38314865 Msa0884620:three_prime_UTR 45.0%
!!! AAATAAATTTTAGATAATTA+GGG - chr6_1:38313434-38313453 None:intergenic 5.0%
!!! AAAATAAATTTTAGATAATT+AGG - chr6_1:38313435-38313454 None:intergenic 5.0%
AAGGATTGGGTGGCTACTAG+AGG - chr6_1:38312377-38312396 None:intergenic 50.0%
GAGGGTTGAGTTTGGAGAGA+GGG - chr6_1:38312532-38312551 None:intergenic 50.0%
AGAGGGTTGAGTTTGGAGAG+AGG - chr6_1:38312533-38312552 None:intergenic 50.0%
ACGGCTTCCAATTGCGTAGA+GGG - chr6_1:38312550-38312569 None:intergenic 50.0%
AACGGCTTCCAATTGCGTAG+AGG - chr6_1:38312551-38312570 None:intergenic 50.0%
!! CGACGCCGTTTTAGATGATC+CGG + chr6_1:38312641-38312660 Msa0884620:CDS 50.0%
CAAATCTCCTCGATCCCTTG+AGG - chr6_1:38314033-38314052 None:intergenic 50.0%
CTAGGAACACTAGGTGACAC+TGG + chr6_1:38314263-38314282 Msa0884620:CDS 50.0%
GAACACTAGGTGACACTGGT+TGG + chr6_1:38314267-38314286 Msa0884620:CDS 50.0%
GTTAAGTAAGGACCCCCCAT+TGG - chr6_1:38314816-38314835 None:intergenic 50.0%
TGGGGGGTCCTTACTTAACA+AGG + chr6_1:38314817-38314836 Msa0884620:three_prime_UTR 50.0%
AGAGGATACTTAACGGGGAG+TGG - chr6_1:38314844-38314863 None:intergenic 50.0%
!! ATGTAGACGGCATCGACGTC+CGG - chr6_1:38312663-38312682 None:intergenic 55.0%
CAGCAAGAACTGCCCAGTGA+AGG - chr6_1:38312707-38312726 None:intergenic 55.0%
CTAGCTACCTCAAGGGATCG+AGG + chr6_1:38314023-38314042 Msa0884620:intron 55.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 38312310 38315151 38312310 ID=Msa0884620;Name=Msa0884620
chr6_1 mRNA 38312310 38315151 38312310 ID=Msa0884620-mRNA-1;Parent=Msa0884620;Name=Msa0884620-mRNA-1;_AED=0.02;_eAED=0.02;_QI=137|1|1|1|1|1|2|346|363
chr6_1 exon 38312310 38312917 38312310 ID=Msa0884620-mRNA-1:exon:8909;Parent=Msa0884620-mRNA-1
chr6_1 exon 38314185 38315151 38314185 ID=Msa0884620-mRNA-1:exon:8910;Parent=Msa0884620-mRNA-1
chr6_1 five_prime_UTR 38312310 38312446 38312310 ID=Msa0884620-mRNA-1:five_prime_utr;Parent=Msa0884620-mRNA-1
chr6_1 CDS 38312447 38312917 38312447 ID=Msa0884620-mRNA-1:cds;Parent=Msa0884620-mRNA-1
chr6_1 CDS 38314185 38314805 38314185 ID=Msa0884620-mRNA-1:cds;Parent=Msa0884620-mRNA-1
chr6_1 three_prime_UTR 38314806 38315151 38314806 ID=Msa0884620-mRNA-1:three_prime_utr;Parent=Msa0884620-mRNA-1
Gene Sequence

>Msa0884620

ATGGCTCATGAAGAAACAAAACCAATAAGATTTGGAATCCTTGGTTGTGCAGAGATAGCTAGAAAAGTTTCAAGAGCCATAACCCTCTCTCCAAACTCAACCCTCTACGCAATTGGAAGCCGTTCATTAGAGAAAGCAACAAAATTTGCAGCTTCCAATAATTTCCCTTCCCATGCAAAAATATACGGTTCATACGACGCCGTTTTAGATGATCCGGACGTCGATGCCGTCTACATGCCACTTCCAACTAGCTTACACCTTCACTGGGCAGTTCTTGCTGCTCAAAAGAAGAAACACTTGTTGCTTGAGAAACCAGTTGCTTTAAATGTTGGAGAACTTGACAAGATTTTGGAAGCTTGTGAATCTAATGGATTGCAGTATATGGATTCTACTATGTGGATGCATCATCCTAGAACTGAAAAAATGTTTCAGTTTATATCTGATGCTAATCTCTTTGGTTCCCTTCAATCGGTACATGCTACATTTTCTTACCCGGTGAGTCCAGATTTCTTAGCAAAGGACATTCGTGTTAAGCCAGATCTTGATGCACTAGGAACACTAGGTGACACTGGTTGGTATTGCATTAGAGCAATTTTATGGGCTGCAAATTACGAATTACCCAAAACAGCAAAAGCCTTACATAAACCAAAATACAATGAAGCAGGGGTAATTTTGTCTTGTGAAGCTTCTTTGATATGGGAAGATAACAAAGTAGCAACTTTCTTTTGTTCCTTTTTATCAGATATGAATATGGATATAACAGCAATAGGGGCAAAAGGGTCATTGCGTGTTTATGATTATGTTATACCTAATGAAGAGAATGAGGCTTTGTTTAGAACAAGTTCAAATTCTTATTTTGTTGAACTTTCTACTGGTTGGAATCCAAAACCAAGTGAGGTTATAGTTAAAAATGATATTCCACAAGAAGCACTTATGGTTAAGGAATTTGCTCATCTGGTTGGTGGGATTAAGTATGGGAATTCTAAAGCTGACAATAAATGGGCAATTATTAGTAGGAAAACTCAAGTGGTTATTGATGCTGTGAAAGCTTCAATTGATAATGGTCTTCTGCCTGTTCCTATTGAGTATTAG
Protein sequence

>Msa0884620

MAHEETKPIRFGILGCAEIARKVSRAITLSPNSTLYAIGSRSLEKATKFAASNNFPSHAKIYGSYDAVLDDPDVDAVYMPLPTSLHLHWAVLAAQKKKHLLLEKPVALNVGELDKILEACESNGLQYMDSTMWMHHPRTEKMFQFISDANLFGSLQSVHATFSYPVSPDFLAKDIRVKPDLDALGTLGDTGWYCIRAILWAANYELPKTAKALHKPKYNEAGVILSCEASLIWEDNKVATFFCSFLSDMNMDITAIGAKGSLRVYDYVIPNEENEALFRTSSNSYFVELSTGWNPKPSEVIVKNDIPQEALMVKEFAHLVGGIKYGNSKADNKWAIISRKTQVVIDAVKASIDNGLLPVPIEY*