AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0130510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0130510 MtrunA17_Chr1g0198801 98.529 340 2 2 1 339 1 338 0.0 688
Msa0130510 MtrunA17_Chr7g0255961 49.738 191 89 3 142 331 58 242 8.24e-53 174
Msa0130510 MtrunA17_Chr3g0113721 41.935 217 112 4 124 331 9 220 3.97e-49 164
Msa0130510 MtrunA17_Chr4g0041051 40.625 192 103 2 155 339 64 251 4.98e-31 117
Msa0130510 MtrunA17_Chr2g0325521 42.458 179 92 3 145 316 45 219 1.94e-30 115
Msa0130510 MtrunA17_Chr5g0412371 36.145 166 89 3 157 316 43 197 6.12e-30 113
Msa0130510 MtrunA17_Chr5g0427361 34.934 229 107 4 136 327 8 231 1.24e-26 105
Msa0130510 MtrunA17_Chr4g0045901 43.407 182 89 6 143 317 62 236 1.00e-24 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0130510 AT1G68810.1 64.394 264 68 8 96 337 99 358 1.24e-93 283
Msa0130510 AT3G25710.1 49.844 321 96 8 72 333 22 336 5.84e-80 248
Msa0130510 AT3G56770.1 47.059 170 82 4 151 317 37 201 7.00e-43 148
Msa0130510 AT2G41130.1 46.629 178 84 5 143 317 55 224 2.57e-38 137
Msa0130510 AT2G40200.1 42.105 190 89 8 130 311 38 214 1.56e-33 124
Msa0130510 AT4G38070.1 37.324 142 77 3 153 294 87 216 3.61e-25 102
Msa0130510 AT1G01260.1 31.111 180 99 8 38 207 314 478 1.67e-11 65.9
Msa0130510 AT1G01260.2 31.111 180 99 8 38 207 314 478 1.67e-11 65.9
Msa0130510 AT1G01260.3 31.111 180 99 8 38 207 314 478 1.67e-11 65.9
Msa0130510 AT5G57150.1 25.137 183 99 5 166 319 58 231 9.04e-11 62.0

Find 77 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATTATTATATTAGTATA+TGG 0.263249 1_3:-60830262 Msa0130510:three_prime_UTR
AATTAGAATTGATTAAATTA+TGG 0.270913 1_3:-60830318 Msa0130510:three_prime_UTR
ATGCAAGTTTGTTCAATAGA+AGG 0.283417 1_3:-60832132 Msa0130510:CDS
GATGAGTGCTCAAGAAATTA+TGG 0.286315 1_3:-60831984 Msa0130510:CDS
TCAAGACATTGAAAGCATTA+AGG 0.296728 1_3:-60830546 Msa0130510:CDS
TGTTCTAATTGATTCTATAA+TGG 0.302929 1_3:+60832845 None:intergenic
TTCAATGTCTTGATTAGTTC+AGG 0.303436 1_3:+60830556 None:intergenic
ATGCAGGTTCTGAGCATCTT+AGG 0.326044 1_3:-60832078 Msa0130510:CDS
ACTAATATAATAATAATAAT+AGG 0.326615 1_3:+60830268 None:intergenic
GCAGATGATGATGAAGATTA+TGG 0.331671 1_3:-60830643 Msa0130510:CDS
GTTGGAGATGAATCTGCTTC+TGG 0.334849 1_3:-60830385 Msa0130510:CDS
AATAGTGATCATCATATCTT+TGG 0.353704 1_3:-60832313 Msa0130510:CDS
TGATCTTCTTGTATCATACT+TGG 0.359086 1_3:+60832424 None:intergenic
GTAAGATGTTGTTATGTATA+TGG 0.364483 1_3:-60830135 Msa0130510:three_prime_UTR
ACAATGAGGTAATGATAATT+TGG 0.372247 1_3:-60830090 Msa0130510:three_prime_UTR
GACCATGTTTGTTGCAAGAT+TGG 0.409233 1_3:+60832235 None:intergenic
GTTTGTTTCTCCTGTGGTGG+TGG 0.415527 1_3:+60832896 None:intergenic
GATGAAGATTATGGATCAAA+TGG 0.416803 1_3:-60830634 Msa0130510:CDS
TTCAGCTTCACTATGACTCT+TGG 0.417321 1_3:+60831940 None:intergenic
TTATATTAGTATATGGTACT+TGG 0.421823 1_3:-60830255 Msa0130510:three_prime_UTR
TCATCACATTCAGTTGGAAC+TGG 0.424698 1_3:+60830682 None:intergenic
CCTCACTTTGCTGTGATGAT+AGG 0.438078 1_3:-60830591 Msa0130510:CDS
GGATCAGGTTCAGGACCCTT+TGG 0.439350 1_3:-60832031 Msa0130510:CDS
ACACTTACTTTGGTTGTGCT+TGG 0.447565 1_3:+60831863 None:intergenic
CTATTGAACAAACTTGCATA+AGG 0.450006 1_3:+60832136 None:intergenic
GAGAGATAAATCTAAAGCCT+TGG 0.462647 1_3:-60832935 Msa0130510:five_prime_UTR
CTTCAACATGGATCAGGTTC+AGG 0.466813 1_3:-60832040 Msa0130510:CDS
GCCTCATCATCTGTTGTTGA+AGG 0.470688 1_3:+60832341 None:intergenic
CTTTGGCCGCCCACCACCAC+AGG 0.474997 1_3:-60832906 Msa0130510:five_prime_UTR
TGCAAGTTTGTTCAATAGAA+GGG 0.484018 1_3:-60832131 Msa0130510:CDS
TTCTTTGTTTGTTTCTCCTG+TGG 0.494108 1_3:+60832890 None:intergenic
CTAATTGATTCTATAATGGT+GGG 0.495009 1_3:+60832849 None:intergenic
GTTAATTCATCACATTCAGT+TGG 0.495856 1_3:+60830676 None:intergenic
TTCCCTACAGCAGGAGGAGG+GGG 0.504915 1_3:-60832274 Msa0130510:CDS
TCTAATTGATTCTATAATGG+TGG 0.508231 1_3:+60832848 None:intergenic
ATGAGGTAATGATAATTTGG+TGG 0.510319 1_3:-60830087 Msa0130510:three_prime_UTR
TCAGGTTCAGGACCCTTTGG+TGG 0.514868 1_3:-60832028 Msa0130510:CDS
TTTGTTTGTTTCTCCTGTGG+TGG 0.520687 1_3:+60832893 None:intergenic
AGTGATCATCATATCTTTGG+TGG 0.524209 1_3:-60832310 Msa0130510:CDS
ATGTTCCCTACAGCAGGAGG+AGG 0.528325 1_3:-60832277 Msa0130510:CDS
AGGTCTATGTTCCCTACAGC+AGG 0.528893 1_3:-60832283 Msa0130510:CDS
GCTGATATAACAACACTTGG+AGG 0.532615 1_3:-60830508 Msa0130510:CDS
CACCAATCTTGCAACAAACA+TGG 0.533876 1_3:-60832237 Msa0130510:CDS
TTTGCATATGATCATCATGC+AGG 0.539983 1_3:-60832094 Msa0130510:CDS
TCAAGAAATTATGGAGGCTA+AGG 0.546278 1_3:-60831975 Msa0130510:CDS
AATTGATTCTATAATGGTGG+GGG 0.552128 1_3:+60832851 None:intergenic
GATACACTTCAACATGGATC+AGG 0.552437 1_3:-60832046 Msa0130510:CDS
TCATATGCAAATTGAAGAGA+AGG 0.553108 1_3:+60832106 None:intergenic
TAATTGATTCTATAATGGTG+GGG 0.553540 1_3:+60832850 None:intergenic
TTTGTTTCTCCTGTGGTGGT+GGG 0.561793 1_3:+60832897 None:intergenic
ATCATATCTTTGGTGGAGGA+AGG 0.562826 1_3:-60832303 Msa0130510:CDS
TGTTCCCTACAGCAGGAGGA+GGG 0.570414 1_3:-60832276 Msa0130510:CDS
AGAAGCACTCAAAGCTGTGA+TGG 0.573378 1_3:-60830416 Msa0130510:CDS
GTTTCTCCTGTGGTGGTGGG+CGG 0.576111 1_3:+60832900 None:intergenic
AGCTGCGTAGCTTAGCAAGA+TGG 0.576612 1_3:+60831891 None:intergenic
TCCTTCAACAACAGATGATG+AGG 0.577140 1_3:-60832342 Msa0130510:CDS
TGTTGTTGAGGAATAACGAA+AGG 0.577534 1_3:+60832166 None:intergenic
GATCATCATATCTTTGGTGG+AGG 0.579767 1_3:-60832307 Msa0130510:CDS
TTCACCTTGAAAACCACCAA+AGG 0.584286 1_3:+60832015 None:intergenic
GTTCCCTACAGCAGGAGGAG+GGG 0.584625 1_3:-60832275 Msa0130510:CDS
TCACCTTGAAAACCACCAAA+GGG 0.586225 1_3:+60832016 None:intergenic
AAAGGTTCATGAGGGTTGAA+AGG 0.586522 1_3:+60832184 None:intergenic
TAAGGTGAAGCTTGTTGTTG+AGG 0.593376 1_3:+60832154 None:intergenic
ATTAGAACAATATCTATCAA+AGG 0.593991 1_3:-60832831 Msa0130510:intron
AGAGTCATAGTGAAGCTGAA+AGG 0.601072 1_3:-60831937 Msa0130510:CDS
AAAGCTGATATAACAACACT+TGG 0.602897 1_3:-60830511 Msa0130510:CDS
CTGGAAGTGTCAAGAGACAA+AGG 0.603991 1_3:-60830366 Msa0130510:CDS
GCTGTGATGGAGAAGAGTGT+TGG 0.619796 1_3:-60830403 Msa0130510:CDS
CCTATCATCACAGCAAAGTG+AGG 0.634985 1_3:+60830591 None:intergenic
AGTATGATACAAGAAGATCA+AGG 0.636259 1_3:-60832421 Msa0130510:CDS
GGAATAACGAAAGGTTCATG+AGG 0.639813 1_3:+60832175 None:intergenic
TCTATGTTCCCTACAGCAGG+AGG 0.647545 1_3:-60832280 Msa0130510:CDS
GGCGGCCAAAGAAAAGTCCA+AGG 0.663085 1_3:+60832918 None:intergenic
GAATAACGAAAGGTTCATGA+GGG 0.671930 1_3:+60832176 None:intergenic
ATATCAGATACACTTCAACA+TGG 0.699194 1_3:-60832052 Msa0130510:CDS
GAGTGCTCAAGAAATTATGG+AGG 0.703562 1_3:-60831981 Msa0130510:CDS
AACATGATGATGAGACAATG+AGG 0.726295 1_3:-60830104 Msa0130510:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATATATTAGTACTATAT+TGG + chr1_3:60831336-60831355 None:intergenic 10.0%
!! AAATTTATAGTTAATTAAAG+TGG + chr1_3:60832162-60832181 None:intergenic 10.0%
!! AATTAGAATTGATTAAATTA+TGG - chr1_3:60832716-60832735 Msa0130510:intron 10.0%
!!! TTTTTATCTTTTTTTTTTTG+GGG - chr1_3:60832834-60832853 Msa0130510:five_prime_UTR 10.0%
!! ATATATACATATGAATATGA+TGG + chr1_3:60830545-60830564 None:intergenic 15.0%
!! ATATGTATATATATAAACCT+TGG - chr1_3:60830553-60830572 Msa0130510:CDS 15.0%
!!! TTTTATCTTTTTTTTTTTGG+GGG - chr1_3:60832835-60832854 Msa0130510:five_prime_UTR 15.0%
!!! TGTTCTAATTGATTCTATAA+TGG + chr1_3:60830192-60830211 None:intergenic 20.0%
!! ATTAGAACAATATCTATCAA+AGG - chr1_3:60830203-60830222 Msa0130510:three_prime_UTR 20.0%
!! ACAAGGAATAAAAATGAAAA+GGG + chr1_3:60830299-60830318 None:intergenic 20.0%
!! ACAAAATAAAACCTAGATAT+AGG - chr1_3:60830488-60830507 Msa0130510:CDS 20.0%
!!! AGATATTTTGAATCTACAAA+AGG + chr1_3:60831447-60831466 None:intergenic 20.0%
!!! GATTTTGATTTTGTTGTTTA+TGG + chr1_3:60831482-60831501 None:intergenic 20.0%
!!! AATAGTGGATATATTTTGAA+TGG + chr1_3:60831949-60831968 None:intergenic 20.0%
!! TATGATATAAGAATGTGAAA+TGG + chr1_3:60831973-60831992 None:intergenic 20.0%
!! ATAGATCTGATATATACATA+AGG + chr1_3:60832014-60832033 None:intergenic 20.0%
!! TGAAAATATTTACAATCTCA+TGG + chr1_3:60832047-60832066 None:intergenic 20.0%
!! TTATATTAGTATATGGTACT+TGG - chr1_3:60832779-60832798 Msa0130510:intron 20.0%
!!! TCTTTTCTTTTCTTTTTTCA+TGG - chr1_3:60832870-60832889 Msa0130510:five_prime_UTR 20.0%
!! TAATTGATTCTATAATGGTG+GGG + chr1_3:60830187-60830206 None:intergenic 25.0%
!! CTAATTGATTCTATAATGGT+GGG + chr1_3:60830188-60830207 None:intergenic 25.0%
!! TCTAATTGATTCTATAATGG+TGG + chr1_3:60830189-60830208 None:intergenic 25.0%
!!! AAATGAAAAGGGTATTTGAA+AGG + chr1_3:60830288-60830307 None:intergenic 25.0%
! CACAAGGAATAAAAATGAAA+AGG + chr1_3:60830300-60830319 None:intergenic 25.0%
! TATATATAAACCTTGGAGTT+TGG - chr1_3:60830560-60830579 Msa0130510:CDS 25.0%
! AATAGTGATCATCATATCTT+TGG - chr1_3:60830721-60830740 Msa0130510:CDS 25.0%
! TTACAACTATACACTTACTT+TGG + chr1_3:60831184-60831203 None:intergenic 25.0%
!! GGTTTGCATTTTAAATTTCT+TGG + chr1_3:60831292-60831311 None:intergenic 25.0%
! GGCATATATTGAATTCAAAA+AGG + chr1_3:60831426-60831445 None:intergenic 25.0%
!!! TAGTAACCTTTTAGAAAATG+AGG - chr1_3:60831505-60831524 Msa0130510:intron 25.0%
!!! CACATTGTTCTCTTTTTTTT+TGG - chr1_3:60831589-60831608 Msa0130510:intron 25.0%
!!! TGTAACTATATGTTTTGTTG+TGG - chr1_3:60831641-60831660 Msa0130510:intron 25.0%
!!! GTAACTATATGTTTTGTTGT+GGG - chr1_3:60831642-60831661 Msa0130510:intron 25.0%
! TGAAAATATATTGTCTTTGC+CGG - chr1_3:60831806-60831825 Msa0130510:intron 25.0%
! TGGAGAATGAAAAGAAATAA+AGG - chr1_3:60831856-60831875 Msa0130510:intron 25.0%
! ATCTGATATATACATAAGGT+TGG + chr1_3:60832010-60832029 None:intergenic 25.0%
! ATATTTACAATCTCATGGTA+GGG + chr1_3:60832042-60832061 None:intergenic 25.0%
! AATATTTACAATCTCATGGT+AGG + chr1_3:60832043-60832062 None:intergenic 25.0%
!!! AAGAATGTTTTGTTCATTAC+TGG - chr1_3:60832556-60832575 Msa0130510:intron 25.0%
! GTAAGATGTTGTTATGTATA+TGG - chr1_3:60832899-60832918 Msa0130510:five_prime_UTR 25.0%
! ACAATGAGGTAATGATAATT+TGG - chr1_3:60832944-60832963 Msa0130510:five_prime_UTR 25.0%
!!! GTTTTATTTTTACATGTGTG+TGG - chr1_3:60832969-60832988 Msa0130510:five_prime_UTR 25.0%
! AATTGATTCTATAATGGTGG+GGG + chr1_3:60830186-60830205 None:intergenic 30.0%
GGTATTTGAAAGGACAAAAT+GGG + chr1_3:60830278-60830297 None:intergenic 30.0%
AAATGTCAGAACCTATATCT+AGG + chr1_3:60830502-60830521 None:intergenic 30.0%
! TGTTTTATATCCAAACTCCA+AGG + chr1_3:60830573-60830592 None:intergenic 30.0%
TTGGAGTTTGGATATAAAAC+AGG - chr1_3:60830572-60830591 Msa0130510:CDS 30.0%
TGATCTTCTTGTATCATACT+TGG + chr1_3:60830613-60830632 None:intergenic 30.0%
AGTATGATACAAGAAGATCA+AGG - chr1_3:60830613-60830632 Msa0130510:CDS 30.0%
CTATTGAACAAACTTGCATA+AGG + chr1_3:60830901-60830920 None:intergenic 30.0%
! ATGCAAGTTTGTTCAATAGA+AGG - chr1_3:60830902-60830921 Msa0130510:intron 30.0%
! TGCAAGTTTGTTCAATAGAA+GGG - chr1_3:60830903-60830922 Msa0130510:intron 30.0%
TCATATGCAAATTGAAGAGA+AGG + chr1_3:60830931-60830950 None:intergenic 30.0%
ATATCAGATACACTTCAACA+TGG - chr1_3:60830982-60831001 Msa0130510:intron 30.0%
!! TTGAGTTGAGATCTTTGATT+AGG + chr1_3:60831313-60831332 None:intergenic 30.0%
! TTTGCACCTCATTTTCTAAA+AGG + chr1_3:60831514-60831533 None:intergenic 30.0%
! GCTTTTGTTAACATCCTTTT+TGG - chr1_3:60831553-60831572 Msa0130510:intron 30.0%
GAATTAGTGTTAATGTCTCT+TGG - chr1_3:60831737-60831756 Msa0130510:intron 30.0%
TGACTGATCAACATATTATC+CGG + chr1_3:60831828-60831847 None:intergenic 30.0%
!! AGTGGATATATTTTGAATGG+TGG + chr1_3:60831946-60831965 None:intergenic 30.0%
AGAATGTGAAATGGAAATAG+TGG + chr1_3:60831964-60831983 None:intergenic 30.0%
! GATATATACATAAGGTTGGT+AGG + chr1_3:60832006-60832025 None:intergenic 30.0%
GTTAATTCATCACATTCAGT+TGG + chr1_3:60832361-60832380 None:intergenic 30.0%
GATGAAGATTATGGATCAAA+TGG - chr1_3:60832400-60832419 Msa0130510:CDS 30.0%
! TTCAATGTCTTGATTAGTTC+AGG + chr1_3:60832481-60832500 None:intergenic 30.0%
!! TCAAGACATTGAAAGCATTA+AGG - chr1_3:60832488-60832507 Msa0130510:intron 30.0%
AAAGCTGATATAACAACACT+TGG - chr1_3:60832523-60832542 Msa0130510:intron 30.0%
!!! TATCTTTTTTTTTTTGGGGG+TGG - chr1_3:60832838-60832857 Msa0130510:five_prime_UTR 30.0%
ATGAGGTAATGATAATTTGG+TGG - chr1_3:60832947-60832966 Msa0130510:five_prime_UTR 30.0%
! GAGAGATAAATCTAAAGCCT+TGG - chr1_3:60830099-60830118 Msa0130510:three_prime_UTR 35.0%
! TAAAGCCTTGGACTTTTCTT+TGG - chr1_3:60830111-60830130 Msa0130510:three_prime_UTR 35.0%
! TTCTTTGTTTGTTTCTCCTG+TGG + chr1_3:60830147-60830166 None:intergenic 35.0%
GGGTATTTGAAAGGACAAAA+TGG + chr1_3:60830279-60830298 None:intergenic 35.0%
TATAGGCACTTAATTGCACA+AGG + chr1_3:60830316-60830335 None:intergenic 35.0%
AGTGATCATCATATCTTTGG+TGG - chr1_3:60830724-60830743 Msa0130510:CDS 35.0%
GAATAACGAAAGGTTCATGA+GGG + chr1_3:60830861-60830880 None:intergenic 35.0%
TGTTGTTGAGGAATAACGAA+AGG + chr1_3:60830871-60830890 None:intergenic 35.0%
TTTGCATATGATCATCATGC+AGG - chr1_3:60830940-60830959 Msa0130510:intron 35.0%
GATGAGTGCTCAAGAAATTA+TGG - chr1_3:60831050-60831069 Msa0130510:intron 35.0%
TCAAGAAATTATGGAGGCTA+AGG - chr1_3:60831059-60831078 Msa0130510:intron 35.0%
!!! GCAAGAGTGTTTTTTCTAAG+TGG - chr1_3:60831400-60831419 Msa0130510:intron 35.0%
TATAATGTGTGGTAGTTCCA+AGG - chr1_3:60831673-60831692 Msa0130510:intron 35.0%
TGTCTCTTGGTATGATAAAG+AGG - chr1_3:60831750-60831769 Msa0130510:intron 35.0%
!! TTTTCAACTGCATGCTGTTT+TGG + chr1_3:60831778-60831797 None:intergenic 35.0%
GTTGATCAGTCAAACTCAAT+TGG - chr1_3:60831836-60831855 Msa0130510:intron 35.0%
GTTATGTTTGTGACTGACAA+AGG + chr1_3:60831900-60831919 None:intergenic 35.0%
! GCAGATGATGATGAAGATTA+TGG - chr1_3:60832391-60832410 Msa0130510:CDS 35.0%
! AACATGATGATGAGACAATG+AGG - chr1_3:60832930-60832949 Msa0130510:five_prime_UTR 35.0%
! TTTGTTTGTTTCTCCTGTGG+TGG + chr1_3:60830144-60830163 None:intergenic 40.0%
TCCTTCAACAACAGATGATG+AGG - chr1_3:60830692-60830711 Msa0130510:CDS 40.0%
GATCATCATATCTTTGGTGG+AGG - chr1_3:60830727-60830746 Msa0130510:CDS 40.0%
ATCATATCTTTGGTGGAGGA+AGG - chr1_3:60830731-60830750 Msa0130510:CDS 40.0%
CACCAATCTTGCAACAAACA+TGG - chr1_3:60830797-60830816 Msa0130510:intron 40.0%
! GACCATGTTTGTTGCAAGAT+TGG + chr1_3:60830802-60830821 None:intergenic 40.0%
AAAGGTTCATGAGGGTTGAA+AGG + chr1_3:60830853-60830872 None:intergenic 40.0%
GGAATAACGAAAGGTTCATG+AGG + chr1_3:60830862-60830881 None:intergenic 40.0%
!! TAAGGTGAAGCTTGTTGTTG+AGG + chr1_3:60830883-60830902 None:intergenic 40.0%
GATACACTTCAACATGGATC+AGG - chr1_3:60830988-60831007 Msa0130510:intron 40.0%
TCACCTTGAAAACCACCAAA+GGG + chr1_3:60831021-60831040 None:intergenic 40.0%
TTCACCTTGAAAACCACCAA+AGG + chr1_3:60831022-60831041 None:intergenic 40.0%
GAGTGCTCAAGAAATTATGG+AGG - chr1_3:60831053-60831072 Msa0130510:intron 40.0%
TTCAGCTTCACTATGACTCT+TGG + chr1_3:60831097-60831116 None:intergenic 40.0%
AGAGTCATAGTGAAGCTGAA+AGG - chr1_3:60831097-60831116 Msa0130510:intron 40.0%
ACACTTACTTTGGTTGTGCT+TGG + chr1_3:60831174-60831193 None:intergenic 40.0%
!!! AACATCCTTTTTGGCTTGTC+TGG - chr1_3:60831562-60831581 Msa0130510:intron 40.0%
GTATACCAGACAAGCCAAAA+AGG + chr1_3:60831570-60831589 None:intergenic 40.0%
CTAGCTGGCTAAAGAAAAAG+TGG - chr1_3:60831702-60831721 Msa0130510:intron 40.0%
TCATCACATTCAGTTGGAAC+TGG + chr1_3:60832355-60832374 None:intergenic 40.0%
GCTGATATAACAACACTTGG+AGG - chr1_3:60832526-60832545 Msa0130510:intron 40.0%
! TTTGTTTCTCCTGTGGTGGT+GGG + chr1_3:60830140-60830159 None:intergenic 45.0%
GCCTCATCATCTGTTGTTGA+AGG + chr1_3:60830696-60830715 None:intergenic 45.0%
!! ATGCAGGTTCTGAGCATCTT+AGG - chr1_3:60830956-60830975 Msa0130510:intron 45.0%
CTTCAACATGGATCAGGTTC+AGG - chr1_3:60830994-60831013 Msa0130510:intron 45.0%
!! GGTGGTTTTCAAGGTGAAGT+TGG - chr1_3:60831024-60831043 Msa0130510:intron 45.0%
!! GGGAGTGTCTCTATAATGTG+TGG - chr1_3:60831662-60831681 Msa0130510:intron 45.0%
CCTATCATCACAGCAAAGTG+AGG + chr1_3:60832446-60832465 None:intergenic 45.0%
! CCTCACTTTGCTGTGATGAT+AGG - chr1_3:60832443-60832462 Msa0130510:five_prime_UTR 45.0%
!! AGAAGCACTCAAAGCTGTGA+TGG - chr1_3:60832618-60832637 Msa0130510:intron 45.0%
GTTGGAGATGAATCTGCTTC+TGG - chr1_3:60832649-60832668 Msa0130510:intron 45.0%
!! CTGGAAGTGTCAAGAGACAA+AGG - chr1_3:60832668-60832687 Msa0130510:intron 45.0%
!! AATAAAAAAAAAATCTATAT+AGG + chr1_3:60830333-60830352 None:intergenic 5.0%
!!! TATAAATTTTATCTAATTAA+AGG - chr1_3:60832743-60832762 Msa0130510:intron 5.0%
!! ACTAATATAATAATAATAAT+AGG + chr1_3:60832769-60832788 None:intergenic 5.0%
!! ATTATTATTATATTAGTATA+TGG - chr1_3:60832772-60832791 Msa0130510:intron 5.0%
!!! GTTATTATATATTTTTTATT+TGG - chr1_3:60832801-60832820 Msa0130510:intron 5.0%
!!! AATTTTTATCTTTTTTTTTT+TGG - chr1_3:60832832-60832851 Msa0130510:five_prime_UTR 5.0%
!!! ATTTTTATCTTTTTTTTTTT+GGG - chr1_3:60832833-60832852 Msa0130510:five_prime_UTR 5.0%
! GTTTGTTTCTCCTGTGGTGG+TGG + chr1_3:60830141-60830160 None:intergenic 50.0%
AGGTCTATGTTCCCTACAGC+AGG - chr1_3:60830751-60830770 Msa0130510:CDS 50.0%
TCTATGTTCCCTACAGCAGG+AGG - chr1_3:60830754-60830773 Msa0130510:CDS 50.0%
!! GGACCCTTTGGTGGTTTTCA+AGG - chr1_3:60831015-60831034 Msa0130510:intron 50.0%
AGCTGCGTAGCTTAGCAAGA+TGG + chr1_3:60831146-60831165 None:intergenic 50.0%
TGAGCTGAGCTGAGATCTCA+TGG + chr1_3:60832073-60832092 None:intergenic 50.0%
! GCTGTGATGGAGAAGAGTGT+TGG - chr1_3:60832631-60832650 Msa0130510:intron 50.0%
GGCGGCCAAAGAAAAGTCCA+AGG + chr1_3:60830119-60830138 None:intergenic 55.0%
ATGTTCCCTACAGCAGGAGG+AGG - chr1_3:60830757-60830776 Msa0130510:CDS 55.0%
TGTTCCCTACAGCAGGAGGA+GGG - chr1_3:60830758-60830777 Msa0130510:CDS 55.0%
GGATCAGGTTCAGGACCCTT+TGG - chr1_3:60831003-60831022 Msa0130510:intron 55.0%
TCAGGTTCAGGACCCTTTGG+TGG - chr1_3:60831006-60831025 Msa0130510:intron 55.0%
GTTCCAAGGAAGCAGCTAGC+TGG - chr1_3:60831687-60831706 Msa0130510:intron 55.0%
TAGCCAGCTAGCTGCTTCCT+TGG + chr1_3:60831693-60831712 None:intergenic 55.0%
!! GTTTCTCCTGTGGTGGTGGG+CGG + chr1_3:60830137-60830156 None:intergenic 60.0%
GTTCCCTACAGCAGGAGGAG+GGG - chr1_3:60830759-60830778 Msa0130510:CDS 60.0%
TTCCCTACAGCAGGAGGAGG+GGG - chr1_3:60830760-60830779 Msa0130510:CDS 60.0%
CACCCCCTCCTCCTGCTGTA+GGG + chr1_3:60830765-60830784 None:intergenic 65.0%
TCACCCCCTCCTCCTGCTGT+AGG + chr1_3:60830766-60830785 None:intergenic 65.0%
CTTTGGCCGCCCACCACCAC+AGG - chr1_3:60830128-60830147 Msa0130510:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 60830058 60832998 60830058 ID=Msa0130510;Name=Msa0130510
chr1_3 mRNA 60830058 60832998 60830058 ID=Msa0130510-mRNA-1;Parent=Msa0130510;Name=Msa0130510-mRNA-1;_AED=0.00;_eAED=0.00;_QI=189|1|1|1|0.5|0.33|3|281|339
chr1_3 exon 60830058 60830788 60830058 ID=Msa0130510-mRNA-1:exon:20070;Parent=Msa0130510-mRNA-1
chr1_3 exon 60831871 60832462 60831871 ID=Msa0130510-mRNA-1:exon:20069;Parent=Msa0130510-mRNA-1
chr1_3 exon 60832832 60832998 60832832 ID=Msa0130510-mRNA-1:exon:20068;Parent=Msa0130510-mRNA-1
chr1_3 five_prime_UTR 60832832 60832998 60832832 ID=Msa0130510-mRNA-1:five_prime_utr;Parent=Msa0130510-mRNA-1
chr1_3 five_prime_UTR 60832441 60832462 60832441 ID=Msa0130510-mRNA-1:five_prime_utr;Parent=Msa0130510-mRNA-1
chr1_3 CDS 60831871 60832440 60831871 ID=Msa0130510-mRNA-1:cds;Parent=Msa0130510-mRNA-1
chr1_3 CDS 60830339 60830788 60830339 ID=Msa0130510-mRNA-1:cds;Parent=Msa0130510-mRNA-1
chr1_3 three_prime_UTR 60830058 60830338 60830058 ID=Msa0130510-mRNA-1:three_prime_utr;Parent=Msa0130510-mRNA-1
Gene Sequence

>Msa0130510

ATGATACAAGAAGATCAAGGACAGTGTTCATCTCAAACAATCAATAACTTTGAAACTTATCAACAAGAGCAGTTTCTCCTTCAACAACAGATGATGAGGCAACAAAATAGTGATCATCATATCTTTGGTGGAGGAAGGTCTATGTTCCCTACAGCAGGAGGAGGGGGTGATCATCAAGTTTCACCAATCTTGCAACAAACATGGTCTATGCAACAACTTCATCATCATCATCATCCTTTCAACCCTCATGAACCTTTCGTTATTCCTCAACAACAAGCTTCACCTTATGCAAGTTTGTTCAATAGAAGGGTTCCTTCTCTTCAATTTGCATATGATCATCATGCAGGTTCTGAGCATCTTAGGATCATATCAGATACACTTCAACATGGATCAGGTTCAGGACCCTTTGGTGGTTTTCAAGGTGAAGTTGGAAAGATGAGTGCTCAAGAAATTATGGAGGCTAAGGCTCTTGCTGCTTCCAAGAGTCATAGTGAAGCTGAAAGGAGACGCAGAGAAAGAATCAATAACCATCTTGCTAAGCTACGCAGCTTGTTGCCAAGCACAACCAAAACAGACAAAGCATCATTGCTAGCAGAAGTGATACAACATGTAAAAGAATTGAAGCGTCAAACATCATTGATAGCAGAAACAAGTCCAGTTCCAACTGAATGTGATGAATTAACAGTAGATGCTGCAGATGATGATGAAGATTATGGATCAAATGGAAACAAGTTTATCATAAAAGCCTCACTTTGCTGTGATGATAGGTCAGATCTTTTGCCTGAACTAATCAAGACATTGAAAGCATTAAGGTTAAGAACACTCAAAGCTGATATAACAACACTTGGAGGACGTGTTAAGAATGTTTTGTTCATTACTGGTGAAGAAGATGATCATGAATATTGCATTAGTTCAATACAAGAAGCACTCAAAGCTGTGATGGAGAAGAGTGTTGGAGATGAATCTGCTTCTGGAAGTGTCAAGAGACAAAGGACTAATATAATATCAATATCTAATTAA
Protein sequence

>Msa0130510

MIQEDQGQCSSQTINNFETYQQEQFLLQQQMMRQQNSDHHIFGGGRSMFPTAGGGGDHQVSPILQQTWSMQQLHHHHHPFNPHEPFVIPQQQASPYASLFNRRVPSLQFAYDHHAGSEHLRIISDTLQHGSGSGPFGGFQGEVGKMSAQEIMEAKALAASKSHSEAERRRRERINNHLAKLRSLLPSTTKTDKASLLAEVIQHVKELKRQTSLIAETSPVPTECDELTVDAADDDEDYGSNGNKFIIKASLCCDDRSDLLPELIKTLKALRLRTLKADITTLGGRVKNVLFITGEEDDHEYCISSIQEALKAVMEKSVGDESASGSVKRQRTNIISISN*