AlfalfaGEDB | Msa0201250

M. sativa cultivar zhongmu-4 / Msa0201250

KEGG: -
GO: -
PFAM: PF12796.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0201250	KEH37391.1	80.551	653	105	5	1	634	1	650	0.0	1051

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0201250	A0A072V709	80.551	653	105	5	1	634	1	650	0.0	1051

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0135820	Msa0201250	0.803220	3.868201e-49	-8.615850e-47
Msa0141230	Msa0201250	0.804921	1.712873e-49	-8.615850e-47
Msa0201250	Msa0243890	0.828328	9.556743e-55	-8.615850e-47
Msa0201250	Msa0243930	0.828263	9.905315e-55	-8.615850e-47
Msa0201250	Msa0282550	0.833355	5.592020e-56	-8.615850e-47
Msa0201250	Msa0282590	0.826829	2.187821e-54	-8.615850e-47
Msa0201250	Msa0354800	-0.817358	3.436454e-52	-8.615850e-47
Msa0201250	Msa0407910	0.800677	1.288520e-48	-8.615850e-47
Msa0201250	Msa0456200	0.804903	1.727284e-49	-8.615850e-47
Msa0201250	Msa0627410	0.803131	4.035388e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0201250	MtrunA17_Chr2g0297681	77.361	667	126	6	2	646	6	669	0.0	1039
Msa0201250	MtrunA17_Chr2g0297691	76.662	677	131	7	1	653	5	678	0.0	1033
Msa0201250	MtrunA17_Chr2g0297651	76.758	654	129	5	1	634	5	655	0.0	1016
Msa0201250	MtrunA17_Chr2g0297481	80.361	443	80	3	192	634	1	436	0.0	718
Msa0201250	MtrunA17_Chr2g0297491	83.721	344	54	1	291	634	1	342	0.0	584
Msa0201250	MtrunA17_Chr2g0297701	51.953	512	235	5	124	634	33	534	0.0	531
Msa0201250	MtrunA17_Chr2g0297631	66.762	349	84	7	1	321	5	349	5.65e-142	436
Msa0201250	MtrunA17_Chr2g0297711	44.722	521	227	7	129	648	41	501	8.55e-142	424
Msa0201250	MtrunA17_Chr2g0297531	81.159	207	35	3	192	398	1	203	2.64e-111	333
Msa0201250	MtrunA17_Chr2g0297541	68.649	185	29	2	1	156	5	189	3.25e-79	249

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0201250	AT5G35810.1	47.440	293	141	4	345	630	6	292	1.37e-81	262
Msa0201250	AT3G18670.2	31.801	544	298	12	145	624	59	593	3.29e-75	254
Msa0201250	AT3G18670.1	31.755	507	292	7	145	624	59	538	7.15e-74	249
Msa0201250	AT5G04690.2	32.403	466	275	10	206	633	148	611	2.20e-62	219
Msa0201250	AT5G04690.1	32.731	443	270	10	206	633	148	577	2.73e-62	218
Msa0201250	AT5G04700.1	32.475	505	297	12	168	629	112	615	4.19e-62	219
Msa0201250	AT5G04690.3	31.749	463	268	9	206	633	148	597	1.06e-60	214
Msa0201250	AT5G04680.2	31.134	485	286	11	201	637	12	496	2.87e-57	203
Msa0201250	AT5G04680.4	31.134	485	286	11	201	637	135	619	3.22e-56	202
Msa0201250	AT5G04680.3	30.705	482	278	11	201	637	135	605	5.06e-54	196

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Find 116 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACACTACTCATCAAAGG+AGG	0.676865	2_1:-22331709	Msa0201250:CDS
AAAATGCTGCCATTACAAAT+GGG	0.534307	2_1:-22333198	Msa0201250:CDS
AAACCTATGTACAAAAGTAT+TGG	0.433272	2_1:+22330308	None:intergenic
AAAGTATGGTGGCTTTGAAA+TGG	0.431946	2_1:+22335032	None:intergenic
AAATCTGTACCATCAAAGTA+TGG	0.435685	2_1:+22335018	None:intergenic
AAATGTGTGTCGAGTTATTA+TGG	0.238690	2_1:-22331374	Msa0201250:CDS
AACCTATGTACAAAAGTATT+GGG	0.399981	2_1:+22330309	None:intergenic
AACTGCAGCAGCGAACACTC+CGG	0.662595	2_1:+22330645	None:intergenic
AAGGAAGATACAAAATGACA+TGG	0.688802	2_1:-22332937	Msa0201250:CDS
AATAGACAAGAAAATCAATA+TGG	0.415156	2_1:+22330513	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCTGTACCATCAAAGTA+TGG	+	chr2_1:22329922-22329941	None:intergenic	30.0%
TGGTAAAGATAAGAAGATCA+TGG	-	chr2_1:22330044-22330063	Msa0201250:three_prime_UTR	30.0%
CTTAAGATCATCTGGATTTA+TGG	+	chr2_1:22330106-22330125	None:intergenic	30.0%
ACAATTCTCTTAAGATCATC+TGG	+	chr2_1:22330114-22330133	None:intergenic	30.0%
AACAAAAGAGAAAAGGAAAC+AGG	-	chr2_1:22330261-22330280	Msa0201250:three_prime_UTR	30.0%
GTGCATATATTTATCCAAAG+TGG	-	chr2_1:22330358-22330377	Msa0201250:CDS	30.0%
TTAATATATGCTAGCCACTT+TGG	+	chr2_1:22330375-22330394	None:intergenic	30.0%
AACCACTAGATTAAACACTT+TGG	-	chr2_1:22330790-22330809	Msa0201250:CDS	30.0%
AAGTAACTAGAAAGATGTAG+AGG	-	chr2_1:22331232-22331251	Msa0201250:intron	30.0%
AGTGAAGATGTATGGAAATA+AGG	-	chr2_1:22331257-22331276	Msa0201250:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	22329889	22335070	22329889	ID=Msa0201250;Name=Msa0201250
chr2_1	mRNA	22329889	22335070	22329889	ID=Msa0201250-mRNA-1;Parent=Msa0201250;Name=Msa0201250-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|0.8\|0.83\|1\|0.8\|0.83\|6\|423\|654
chr2_1	exon	22329889	22330833	22329889	ID=Msa0201250-mRNA-1:exon:8901;Parent=Msa0201250-mRNA-1
chr2_1	exon	22331368	22331789	22331368	ID=Msa0201250-mRNA-1:exon:8900;Parent=Msa0201250-mRNA-1
chr2_1	exon	22331871	22332035	22331871	ID=Msa0201250-mRNA-1:exon:8899;Parent=Msa0201250-mRNA-1
chr2_1	exon	22332849	22333331	22332849	ID=Msa0201250-mRNA-1:exon:8898;Parent=Msa0201250-mRNA-1
chr2_1	exon	22334320	22334423	22334320	ID=Msa0201250-mRNA-1:exon:8897;Parent=Msa0201250-mRNA-1
chr2_1	exon	22334802	22335070	22334802	ID=Msa0201250-mRNA-1:exon:8896;Parent=Msa0201250-mRNA-1
chr2_1	CDS	22334802	22335070	22334802	ID=Msa0201250-mRNA-1:cds;Parent=Msa0201250-mRNA-1
chr2_1	CDS	22334320	22334423	22334320	ID=Msa0201250-mRNA-1:cds;Parent=Msa0201250-mRNA-1
chr2_1	CDS	22332849	22333331	22332849	ID=Msa0201250-mRNA-1:cds;Parent=Msa0201250-mRNA-1
chr2_1	CDS	22331871	22332035	22331871	ID=Msa0201250-mRNA-1:cds;Parent=Msa0201250-mRNA-1
chr2_1	CDS	22331368	22331789	22331368	ID=Msa0201250-mRNA-1:cds;Parent=Msa0201250-mRNA-1
chr2_1	CDS	22330312	22330833	22330312	ID=Msa0201250-mRNA-1:cds;Parent=Msa0201250-mRNA-1
chr2_1	three_prime_UTR	22329889	22330311	22329889	ID=Msa0201250-mRNA-1:three_prime_utr;Parent=Msa0201250-mRNA-1

Gene Sequence

>Msa0201250

ATGGTTGAAGGAAGATCCATTTCAAAGCCACCATACTTTGATGGTACAGATTTAACTGAGTGGAGGGAACTTATGAAGATGTTCATACAATCTGTTGACTTCGAAGTTTGGCCTGTGATTGAGAATGGATCAAAACTTCCAAAAACAATAATAAATGGTAAAGATAAGAAGATCATGGAACTAGAAGCAAAAGCTAAATATATATTATGTTGTGCCATAAATCCAGATGATCTTAAGAGAATTGTAAGTTGTCAGACGGCAAAGGAAATGAGAGTGACAACCTCCGATGTCAATTCTAAACCTCCAGTATCACAACAAACTCCGGCATCAGACCTATACAACGCGCCTAATCCTGAGTTTTTAGAGGAAACAAGGGATGATCAAAGGAAAAGGTTTCTCAAGTTTTGTGTTCCCATTCACAAGTATGCTCTAAATGGAAACTGGCCAGCAGCTAAGCGCATCATAGACGAAGAGAATAAGCTAAAAAATGCTGCCATTACAAATGGGTGGCCTACACTATTGCATATTGCTGCAGGTGCTAACCACATTCACTTTGTGGTGGAGTTATTGAAATTGCTTGATGGCAATGATATAGCATTGCAAGATATGAATGGAAACACTGCTTTCTGTATTGCTGCTGCTGCTGGAAATATTGAAATTGTTGACTTAATGCTGAATAGAAACCCCATTTTACCAATTATAAGAGGTGGAAATGGAAATACTCCTATTCAGTATGCTGCTTTGCAAGGAAGATACAAAATGACATGGCATCTATATGATAAAACCATACATCATTTTGAAGAGAAGAAGGACTGGGAGTTATTGTTCTTTGCTTGTATCTACACTGGCATCTATGACTTAGCCTTCAAAATGGTAAGAGACAATAAAGAACTAGCTTTTGCATGTGATGTGAATGAAGAGACGGCTTTACATCTCTTGGCTAAAAATCAAATGCCATCGGATTCTTCATGTCATTGTCCAGATAATGATAATGATCATAATCACATCATGATCAATCCTGACCTGAAAAATCACATGGTTTTTCAATTGGTTAAATTTCTTTGGACCACGATTCTTAAAAAACACTACTCATCAAAGGAGGAGTTAAATAAAATCAGAAATGAACCTTCTCAACTAATATTTGATGCTGCTGAAGTTGGAAATTTTGGGTTCTTATCTGAGCTTATTAGTGCCCATCCAAGCTTGATATGGGATGTGAATAGCAAAAATCAAACTATATTACACATAGCTGTCTTGCATCGACATGCTAGTATCTTCAATCTCGTACATCAAATAGGGCATATTAAGGGTATCATAGTGACATATGCTGATGATGAAGAGAACACTTTATTGCACTTGGCTGCAAGATTAGCACCACCGAGTCAACTTGAATTGGTTTCTGGAGCTGCTTTTCAAATGTGTGTCGAGTTATTATGGTTCGAGAATGTAAAGAAGATAATGTTGCCAGCACAAATAAAGATGAAAAACTCTGAAGACTTGACTGCACAAGAGTTATTCTCAAATGAGCATAAAATACTAAGGAAGGATGCAGAGTCTTGGATGAAAAAAACTGCGGAGTCATGTATGCTAATTTCGACAGTCATTGCAACCGGAGTGTTCGCTGCTGCAGTTACTTTACCAGGCGGTACGGATGACTCTGGAAAACCACACTATTTGAATAAAACATCATTCTTGGTGTTTGCAATCTCAGATGCATCTGCATTCATTTCATCTTCGACTGCCATATTGATTTTCTTGTCTATTCTTGTCTCGCGTTATGGAGAGTATGACTTCTATAAATCACTGCCTATAAAGCTAATATTTGGATTGATCACATTATTCATCTCCATAACAAGCATGATGGTAGCACTTAGTAGTTCCTTCTTCATAACATACAACCATGTTCGATACGGTTTCCTATTTGCATTTCAATATTTTCTTTCCTCCCAATACTTTTGTACATAG

Protein sequence

>Msa0201250

MVEGRSISKPPYFDGTDLTEWRELMKMFIQSVDFEVWPVIENGSKLPKTIINGKDKKIMELEAKAKYILCCAINPDDLKRIVSCQTAKEMRVTTSDVNSKPPVSQQTPASDLYNAPNPEFLEETRDDQRKRFLKFCVPIHKYALNGNWPAAKRIIDEENKLKNAAITNGWPTLLHIAAGANHIHFVVELLKLLDGNDIALQDMNGNTAFCIAAAAGNIEIVDLMLNRNPILPIIRGGNGNTPIQYAALQGRYKMTWHLYDKTIHHFEEKKDWELLFFACIYTGIYDLAFKMVRDNKELAFACDVNEETALHLLAKNQMPSDSSCHCPDNDNDHNHIMINPDLKNHMVFQLVKFLWTTILKKHYSSKEELNKIRNEPSQLIFDAAEVGNFGFLSELISAHPSLIWDVNSKNQTILHIAVLHRHASIFNLVHQIGHIKGIIVTYADDEENTLLHLAARLAPPSQLELVSGAAFQMCVELLWFENVKKIMLPAQIKMKNSEDLTAQELFSNEHKILRKDAESWMKKTAESCMLISTVIATGVFAAAVTLPGGTDDSGKPHYLNKTSFLVFAISDASAFISSSTAILIFLSILVSRYGEYDFYKSLPIKLIFGLITLFISITSMMVALSSSFFITYNHVRYGFLFAFQYFLSSQYFCT*