AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0228530


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0228530 MtrunA17_Chr4g0057641 85.714 140 4 1 1 124 1 140 6.91e-75 218
Msa0228530 MtrunA17_Chr4g0057661 83.571 140 7 1 1 124 1 140 6.55e-73 213
Msa0228530 MtrunA17_Chr4g0057651 82.857 140 8 1 1 124 1 140 1.34e-71 210
Msa0228530 MtrunA17_Chr3g0116151 63.571 140 35 1 1 124 1 140 1.21e-52 162
Msa0228530 MtrunA17_Chr3g0116141 64.234 137 33 1 4 124 3 139 3.32e-52 161
Msa0228530 MtrunA17_Chr6g0459231 50.000 134 51 1 6 123 8 141 3.59e-40 130
Msa0228530 MtrunA17_Chr7g0225921 49.630 135 51 2 6 123 6 140 9.03e-39 127
Msa0228530 MtrunA17_Chr8g0373691 48.092 131 51 1 10 123 57 187 1.47e-29 105
Msa0228530 MtrunA17_Chr3g0111371 38.060 134 64 2 6 123 5 135 5.13e-23 87.0
Msa0228530 MtrunA17_Chr8g0392951 35.556 135 71 1 6 124 24 158 1.54e-20 81.6
Msa0228530 MtrunA17_Chr5g0434951 35.075 134 71 1 6 123 4 137 4.17e-20 79.7
Msa0228530 MtrunA17_Chr8g0391691 38.636 132 64 2 6 120 36 167 9.14e-20 80.1
Msa0228530 MtrunA17_Chr1g0161211 37.778 135 67 2 6 123 46 180 1.49e-18 77.0
Msa0228530 MtrunA17_Chr4g0033601 34.058 138 72 3 6 124 5 142 1.36e-16 70.9
Msa0228530 MtrunA17_Chr1g0169581 29.197 137 78 2 6 123 4 140 1.52e-16 70.9
Msa0228530 MtrunA17_Chr6g0482731 33.333 135 73 2 6 123 26 160 2.89e-15 68.2
Msa0228530 MtrunA17_Chr1g0169621 27.778 162 73 3 6 123 4 165 8.31e-15 66.6
Msa0228530 MtrunA17_Chr7g0245911 33.333 150 74 4 1 124 42 191 1.12e-14 67.0
Msa0228530 MtrunA17_Chr4g0044961 32.090 134 74 2 6 123 57 189 1.07e-13 64.3
Msa0228530 MtrunA17_Chr7g0256701 29.932 147 73 5 4 124 71 213 6.54e-13 62.8
Msa0228530 MtrunA17_Chr6g0460211 32.143 140 69 4 6 123 12 147 1.47e-12 60.5
Msa0228530 MtrunA17_Chr5g0440931 32.143 140 69 4 6 123 12 147 1.51e-12 60.5
Msa0228530 MtrunA17_Chr7g0254731 31.429 140 70 4 6 123 12 147 1.74e-12 60.5
Msa0228530 MtrunA17_Chr3g0093271 32.143 140 69 4 6 123 63 198 3.84e-12 60.5
Msa0228530 MtrunA17_Chr3g0121191 31.034 145 53 2 6 123 93 217 4.71e-12 60.5
Msa0228530 MtrunA17_Chr1g0188171 32.143 140 70 3 5 123 11 146 9.35e-12 58.5
Msa0228530 MtrunA17_Chr5g0402331 29.630 135 78 2 6 123 48 182 1.90e-11 58.2
Msa0228530 MtrunA17_Chr7g0256171 30.435 138 77 3 6 124 5 142 5.53e-11 56.6
Msa0228530 MtrunA17_Chr5g0402361 29.630 135 78 2 6 123 220 354 5.78e-11 58.2
Msa0228530 MtrunA17_Chr1g0166281 31.618 136 76 2 5 123 11 146 8.68e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0228530 AT1G76650.1 41.912 136 63 1 4 123 41 176 4.20e-28 101
Msa0228530 AT1G76650.3 41.912 136 63 1 4 123 41 176 4.20e-28 101
Msa0228530 AT5G42380.1 41.176 136 62 2 6 123 49 184 1.19e-24 93.2
Msa0228530 AT1G76650.2 40.441 136 55 2 4 123 41 166 2.87e-24 91.7
Msa0228530 AT1G76640.1 38.849 139 66 2 4 123 20 158 4.18e-24 90.9
Msa0228530 AT1G24620.1 40.741 135 63 2 6 123 37 171 4.39e-23 89.0
Msa0228530 AT3G50770.1 39.161 143 65 2 3 123 61 203 5.46e-23 89.4
Msa0228530 AT3G01830.1 36.765 136 69 3 6 124 11 146 1.13e-19 79.3
Msa0228530 AT1G73630.1 33.577 137 72 2 3 123 17 150 9.31e-19 77.4
Msa0228530 AT1G18210.2 33.824 136 77 1 1 123 18 153 1.67e-18 76.6
Msa0228530 AT1G18210.1 33.824 136 77 1 1 123 18 153 1.67e-18 76.6
Msa0228530 AT5G37770.1 30.935 139 75 2 6 123 17 155 1.37e-17 74.3
Msa0228530 AT5G37770.2 30.935 139 75 2 6 123 17 155 1.37e-17 74.3
Msa0228530 AT1G66400.1 29.927 137 77 2 6 123 15 151 1.62e-17 73.9
Msa0228530 AT2G15680.1 34.815 135 69 3 6 123 50 182 1.28e-14 67.0
Msa0228530 AT4G03290.1 33.333 141 72 3 6 124 5 145 7.86e-14 64.3
Msa0228530 AT5G17470.1 31.618 136 70 3 7 123 7 138 2.23e-13 62.8
Msa0228530 AT1G66410.1 32.624 141 67 4 6 123 12 147 8.21e-13 61.6
Msa0228530 AT5G37780.1 32.624 141 67 4 6 123 12 147 8.21e-13 61.6
Msa0228530 AT4G12860.1 30.935 139 73 2 6 123 5 141 8.61e-13 61.6
Msa0228530 AT3G59440.1 30.714 140 79 2 3 124 48 187 8.68e-13 62.4
Msa0228530 AT1G05990.1 32.374 139 74 3 6 124 5 143 8.83e-13 61.6
Msa0228530 AT5G21274.1 32.857 140 68 4 6 123 12 147 9.73e-13 61.2
Msa0228530 AT3G56800.1 32.143 140 69 4 6 123 12 147 1.50e-12 60.8
Msa0228530 AT2G41110.1 32.143 140 69 4 6 123 12 147 1.50e-12 60.8
Msa0228530 AT2G27030.1 32.143 140 69 4 6 123 12 147 1.50e-12 60.8
Msa0228530 AT3G22930.1 32.117 137 75 3 5 123 34 170 1.94e-12 61.2
Msa0228530 AT3G43810.1 32.143 140 69 4 6 123 12 147 2.02e-12 60.5
Msa0228530 AT2G27030.3 32.143 140 69 4 6 123 12 147 2.73e-12 60.8
Msa0228530 AT3G43810.2 32.143 140 69 4 6 123 12 147 3.59e-12 60.8
Msa0228530 AT3G07490.1 32.609 138 74 3 6 124 5 142 3.83e-12 59.7
Msa0228530 AT3G10190.1 28.571 140 82 3 3 124 67 206 7.97e-12 60.1
Msa0228530 AT2G36180.1 30.597 134 73 3 10 123 4 137 1.90e-11 57.8
Msa0228530 AT2G43290.1 29.371 143 77 3 6 124 65 207 1.93e-11 58.9
Msa0228530 AT4G14640.1 32.374 139 69 3 6 123 13 147 4.07e-11 57.0

Find 41 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCGTGTTAAGTAAATTTC+TGG 0.177720 2_1:+81782001 None:intergenic
GGAGGATTTAATAACGCTTA+TGG 0.268235 2_1:-81781546 Msa0228530:CDS
TTAGGCCTATAGCGGTTTAT+AGG 0.314273 2_1:+81781901 None:intergenic
TGAATTTAACTTTGTATAGT+TGG 0.323358 2_1:-81781808 Msa0228530:five_prime_UTR
CATCACTTTACATTCTTCAA+TGG 0.327118 2_1:+81781394 None:intergenic
GATGATTGGAAGGTTTGATT+TGG 0.354750 2_1:-81781375 Msa0228530:CDS
TTAATAACGCTTATGGAATC+CGG 0.394530 2_1:-81781539 Msa0228530:CDS
AGATTTCACCGAACGAGTTA+AGG 0.395728 2_1:-81781664 Msa0228530:CDS
AAGAAAAGTTGAAGGATTTG+AGG 0.418092 2_1:-81781508 Msa0228530:CDS
TAACTCCTATAAACCGCTAT+AGG 0.418927 2_1:-81781906 Msa0228530:five_prime_UTR
GGAAATTGCTATTGAGGCAT+TGG 0.422915 2_1:-81781597 Msa0228530:CDS
TGGTGATGGGCTATTGAGTT+TGG 0.423743 2_1:-81781567 Msa0228530:CDS
AGAAAAGTTGAAGGATTTGA+GGG 0.426919 2_1:-81781507 Msa0228530:CDS
TGTGTTGGCTATGAAACAAT+TGG 0.433319 2_1:+81781944 None:intergenic
AGGAGTCGAATGACGAAGAT+TGG 0.473202 2_1:-81781644 Msa0228530:CDS
ATTCGACTCCTTAACTCGTT+CGG 0.474759 2_1:+81781656 None:intergenic
GAAGAATGTAAAGTGATGAT+TGG 0.486801 2_1:-81781389 Msa0228530:CDS
ATCAATGAAATCAAACAGTT+AGG 0.489044 2_1:+81781883 None:intergenic
TAATAACGCTTATGGAATCC+GGG 0.494077 2_1:-81781538 Msa0228530:CDS
AGAAGTGGAAATTGCTATTG+AGG 0.499700 2_1:-81781603 Msa0228530:CDS
TATATATTGTTCGCAATCAA+AGG 0.500104 2_1:+81781754 None:intergenic
AATGTAAAGTGATGATTGGA+AGG 0.500397 2_1:-81781385 Msa0228530:CDS
AGAGTTTCAATTGAAAGAAG+TGG 0.502556 2_1:-81781618 Msa0228530:CDS
TGAAGAATTCAGAATCATGA+TGG 0.506151 2_1:-81781330 Msa0228530:CDS
AGGTTTGATTTGGATGGTGA+TGG 0.507936 2_1:-81781365 Msa0228530:CDS
ATTGGAAGGTTTGATTTGGA+TGG 0.510943 2_1:-81781371 Msa0228530:CDS
TGATGGGCTATTGAGTTTGG+AGG 0.520299 2_1:-81781564 Msa0228530:CDS
GTGTTGGCTATGAAACAATT+GGG 0.522846 2_1:+81781945 None:intergenic
GCATTGGATTCTGATGGTGA+TGG 0.540839 2_1:-81781581 Msa0228530:CDS
TACTTTGATGAAGACGGTGA+TGG 0.542295 2_1:-81781689 Msa0228530:CDS
GGGAGAAGAAGAAAAGTTGA+AGG 0.545380 2_1:-81781516 Msa0228530:CDS
CTAAGTTACTTTGATGAAGA+CGG 0.574980 2_1:-81781695 Msa0228530:CDS
AGTCGAATGACGAAGATTGG+CGG 0.582253 2_1:-81781641 Msa0228530:CDS
CATTGGATTCTGATGGTGAT+GGG 0.600002 2_1:-81781580 Msa0228530:CDS
AATTAACATGAGGAGTAACA+TGG 0.611175 2_1:-81781732 Msa0228530:exon
ATTGAGGCATTGGATTCTGA+TGG 0.616237 2_1:-81781587 Msa0228530:CDS
TCAAACAGTTAGGCCTATAG+CGG 0.631415 2_1:+81781893 None:intergenic
AATAACGCTTATGGAATCCG+GGG 0.638897 2_1:-81781537 Msa0228530:CDS
AACAATATATAATTAACATG+AGG 0.645774 2_1:-81781742 Msa0228530:exon
TTGGCTATGAAACAATTGGG+TGG 0.680772 2_1:+81781948 None:intergenic
ATAACGCTTATGGAATCCGG+GGG 0.690847 2_1:-81781536 Msa0228530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAATATATAATTAACATG+AGG - chr2_1:81781601-81781620 Msa0228530:CDS 15.0%
!!! TGAATTTAACTTTGTATAGT+TGG - chr2_1:81781535-81781554 Msa0228530:CDS 20.0%
!!! TTTGAAATGTATGATAATGA+AGG - chr2_1:81781864-81781883 Msa0228530:five_prime_UTR 20.0%
! ATCAATGAAATCAAACAGTT+AGG + chr2_1:81781463-81781482 None:intergenic 25.0%
! TATATATTGTTCGCAATCAA+AGG + chr2_1:81781592-81781611 None:intergenic 25.0%
AAGCGTGTTAAGTAAATTTC+TGG + chr2_1:81781345-81781364 None:intergenic 30.0%
AATTAACATGAGGAGTAACA+TGG - chr2_1:81781611-81781630 Msa0228530:CDS 30.0%
! CTAAGTTACTTTGATGAAGA+CGG - chr2_1:81781648-81781667 Msa0228530:CDS 30.0%
AGAGTTTCAATTGAAAGAAG+TGG - chr2_1:81781725-81781744 Msa0228530:CDS 30.0%
TTAATAACGCTTATGGAATC+CGG - chr2_1:81781804-81781823 Msa0228530:five_prime_UTR 30.0%
AAGAAAAGTTGAAGGATTTG+AGG - chr2_1:81781835-81781854 Msa0228530:five_prime_UTR 30.0%
AGAAAAGTTGAAGGATTTGA+GGG - chr2_1:81781836-81781855 Msa0228530:five_prime_UTR 30.0%
! ATGTATGATAATGAAGGATG+TGG - chr2_1:81781870-81781889 Msa0228530:five_prime_UTR 30.0%
! TGTATGATAATGAAGGATGT+GGG - chr2_1:81781871-81781890 Msa0228530:five_prime_UTR 30.0%
AAGAGGATGTTGAAAAAGTT+GGG - chr2_1:81781915-81781934 Msa0228530:five_prime_UTR 30.0%
CATCACTTTACATTCTTCAA+TGG + chr2_1:81781952-81781971 None:intergenic 30.0%
GAAGAATGTAAAGTGATGAT+TGG - chr2_1:81781954-81781973 Msa0228530:five_prime_UTR 30.0%
AATGTAAAGTGATGATTGGA+AGG - chr2_1:81781958-81781977 Msa0228530:five_prime_UTR 30.0%
TGAAGAATTCAGAATCATGA+TGG - chr2_1:81782013-81782032 Msa0228530:five_prime_UTR 30.0%
!! GTGTTGGCTATGAAACAATT+GGG + chr2_1:81781401-81781420 None:intergenic 35.0%
!! TGTGTTGGCTATGAAACAAT+TGG + chr2_1:81781402-81781421 None:intergenic 35.0%
!!! TGCACACACTTATTTTGTGT+TGG + chr2_1:81781417-81781436 None:intergenic 35.0%
TAACTCCTATAAACCGCTAT+AGG - chr2_1:81781437-81781456 Msa0228530:intron 35.0%
! AGAAGTGGAAATTGCTATTG+AGG - chr2_1:81781740-81781759 Msa0228530:exon 35.0%
GGAGGATTTAATAACGCTTA+TGG - chr2_1:81781797-81781816 Msa0228530:five_prime_UTR 35.0%
TAATAACGCTTATGGAATCC+GGG - chr2_1:81781805-81781824 Msa0228530:five_prime_UTR 35.0%
TAACACCAAAGAGTTTGAAG+AGG - chr2_1:81781898-81781917 Msa0228530:five_prime_UTR 35.0%
AACATCCTCTTCAAACTCTT+TGG + chr2_1:81781906-81781925 None:intergenic 35.0%
GAAGAGGATGTTGAAAAAGT+TGG - chr2_1:81781914-81781933 Msa0228530:five_prime_UTR 35.0%
AGAGGATGTTGAAAAAGTTG+GGG - chr2_1:81781916-81781935 Msa0228530:five_prime_UTR 35.0%
GATGATTGGAAGGTTTGATT+TGG - chr2_1:81781968-81781987 Msa0228530:five_prime_UTR 35.0%
!! ATTGGAAGGTTTGATTTGGA+TGG - chr2_1:81781972-81781991 Msa0228530:five_prime_UTR 35.0%
TTGGCTATGAAACAATTGGG+TGG + chr2_1:81781398-81781417 None:intergenic 40.0%
TTAGGCCTATAGCGGTTTAT+AGG + chr2_1:81781445-81781464 None:intergenic 40.0%
TCAAACAGTTAGGCCTATAG+CGG + chr2_1:81781453-81781472 None:intergenic 40.0%
TACTTTGATGAAGACGGTGA+TGG - chr2_1:81781654-81781673 Msa0228530:CDS 40.0%
AGATTTCACCGAACGAGTTA+AGG - chr2_1:81781679-81781698 Msa0228530:CDS 40.0%
ATTCGACTCCTTAACTCGTT+CGG + chr2_1:81781690-81781709 None:intergenic 40.0%
! GGAAATTGCTATTGAGGCAT+TGG - chr2_1:81781746-81781765 Msa0228530:exon 40.0%
!! ATTGAGGCATTGGATTCTGA+TGG - chr2_1:81781756-81781775 Msa0228530:five_prime_UTR 40.0%
! CATTGGATTCTGATGGTGAT+GGG - chr2_1:81781763-81781782 Msa0228530:five_prime_UTR 40.0%
AATAACGCTTATGGAATCCG+GGG - chr2_1:81781806-81781825 Msa0228530:five_prime_UTR 40.0%
GGGAGAAGAAGAAAAGTTGA+AGG - chr2_1:81781827-81781846 Msa0228530:five_prime_UTR 40.0%
GAGGATGTTGAAAAAGTTGG+GGG - chr2_1:81781917-81781936 Msa0228530:five_prime_UTR 40.0%
!! AGGTTTGATTTGGATGGTGA+TGG - chr2_1:81781978-81781997 Msa0228530:five_prime_UTR 40.0%
AGGAGTCGAATGACGAAGAT+TGG - chr2_1:81781699-81781718 Msa0228530:CDS 45.0%
AGTCGAATGACGAAGATTGG+CGG - chr2_1:81781702-81781721 Msa0228530:CDS 45.0%
! GCATTGGATTCTGATGGTGA+TGG - chr2_1:81781762-81781781 Msa0228530:five_prime_UTR 45.0%
!! TGGTGATGGGCTATTGAGTT+TGG - chr2_1:81781776-81781795 Msa0228530:five_prime_UTR 45.0%
!! TGATGGGCTATTGAGTTTGG+AGG - chr2_1:81781779-81781798 Msa0228530:five_prime_UTR 45.0%
ATAACGCTTATGGAATCCGG+GGG - chr2_1:81781807-81781826 Msa0228530:five_prime_UTR 45.0%
! CAACTTTTCTTCTTCTCCCC+CGG + chr2_1:81781826-81781845 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 81781325 81782040 81781325 ID=Msa0228530;Name=Msa0228530
chr2_1 mRNA 81781325 81782040 81781325 ID=Msa0228530-mRNA-1;Parent=Msa0228530;Name=Msa0228530-mRNA-1;_AED=0.04;_eAED=0.04;_QI=293|0|0.5|1|0|0|2|0|124
chr2_1 exon 81781325 81781445 81781325 ID=Msa0228530-mRNA-1:exon:24469;Parent=Msa0228530-mRNA-1
chr2_1 exon 81781494 81782040 81781494 ID=Msa0228530-mRNA-1:exon:24468;Parent=Msa0228530-mRNA-1
chr2_1 five_prime_UTR 81781748 81782040 81781748 ID=Msa0228530-mRNA-1:five_prime_utr;Parent=Msa0228530-mRNA-1
chr2_1 CDS 81781494 81781747 81781494 ID=Msa0228530-mRNA-1:cds;Parent=Msa0228530-mRNA-1
chr2_1 CDS 81781325 81781445 81781325 ID=Msa0228530-mRNA-1:cds;Parent=Msa0228530-mRNA-1
Gene Sequence

>Msa0228530

ATGAGGAGTAACATGGAATTTGAGCGTGTACTAAGTTACTTTGATGAAGACGGTGATGGAAAGATTTCACCGAACGAGTTAAGGAGTCGAATGACGAAGATTGGCGGAGAGTTTCAATTGAAAGAAGTGGAAATTGCTATTGAGGCATTGGATTCTGATGGTGATGGGCTATTGAGTTTGGAGGATTTAATAACGCTTATGGAATCCGGGGGAGAAGAAGAAAAGTTGAAGGATTTGAGGGAAGCTTTTGAAATGATGTTGAAAAAGTTGGGGGATTCTAAGTCCATTGAAGAATGTAAAGTGATGATTGGAAGGTTTGATTTGGATGGTGATGGTGTGCTTAGTTTTGAAGAATTCAGAATCATGATGGAGTGA

Protein sequence

>Msa0228530

MRSNMEFERVLSYFDEDGDGKISPNELRSRMTKIGGEFQLKEVEIAIEALDSDGDGLLSLEDLITLMESGGEEEKLKDLREAFEMMLKKLGDSKSIEECKVMIGRFDLDGDGVLSFEEFRIMME*